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CHLOROPLASTS, CALVIN CYCLE, PHOTOSYNTHETIC ELECTRON TRANSFER AND PHOTOPHOSPHORYLATION (based on Chapter 19 and 20 of Stryer ) Slide 2 Photosynthesis Slide 3 Light driven transfer of electron across a membrane Results in a proton gradient which drives the bonding of ADP and Pi to form ATP Important in the production of Oxygen and the generation of carbon compounds that make aerobic metabolism possible CO 2 + H 2 O (CH 2 O) + O 2 Light Slide 4 Photosynthetic yield Huge amounts of free energy are stored annually amounting to more than 10 10 tons of carbon in the form of carbohydrate If a years yield were amassed in the form of sugar cane, it would form a heap over two miles high and with a base 43 square miles - G.E.Fogge Slide 5 Photosynthesis takes place in chloroplasts Slide 6 STRUCTURE OF CHLOROPLASTS Typically 5um long. Surrounded by chloroplast envelope with outer and inner membrane and intermembrane space. Slide 7 Inside is the stroma containing soluble enzymes, membranous structures called thylakoids, and a space inside the membranes called the lumen. Thylakoid membranes contain photosynthetic electron transfer chain and ATP synthase for photophosphorylation. They use light energy to synthesise NADPH and ATP (light reactions). The NADPH and ATP are used by the (Benson)-Calvin cycle to convert CO 2 into sugar (dark reactions). The Calvin cycle is located in the stroma Slide 8 PROKARYOTIC ORIGIN OF CHLOROPLASTS Phototrophic bacteria similar to cyanobacteria entered into an endosymbiotic association with the eukaryotic ancestors of algae and higher plants. Chloroplasts are no longer autonomous since most of their proteins are synthesised by nuclear genes. Slide 9 Cyclic tetrapyrrole Chlorophyll - photoreceptor Slide 10 Photoinduced charge separation Slide 11 CHLOROPHYLLS AND THE REACTION CENTRE The principal photoreceptor in chloroplasts is chlorophyll a bound to integral membrane proteins. Chlorophyll has two roles in trapping solar energy a) most chlorophylls absorb light energy and transfer it to a special pair of chlorophylls in a protein complex called a reaction centre. b) only the few chlorophylls forming the special pair mediate the transformation of absorbed light energy into chemical energy Slide 12 Photosynthetic bacteria and photosynthetic reaction centres of green plants (PSI and PSII) have a common core Cytochrome L M H Prosthetic groups C Slide 13 Electron chain in the photosynthetic bacterial reaction centre Slide 14 Slide 15 Slide 16 Cytochrome heme Slide 17 Slide 18 Slide 19 Slide 20 Slide 21 Two photosystems generate a proton gradient and NADPH in oxygenic photosynthesis Slide 22 Slide 23 Slide 24 Slide 25 Slide 26 PSII transfers electrons from water to plastoquinone and generates a proton gradient Slide 27 Cytochrome bf links photosystem II to photosystem I Slide 28 Slide 29 PSI uses light energy to generate reduced Ferredoxin Slide 30 PHOTOSYNTHETIC ELECTRON TRANSFER CHAIN Slide 31 Consists of three transmembrane protein complexes, two reaction centres (Photosystem II [PSII] and Photosystem I) and a cytochrome bf complex analogous to Complex III. These are linked by two mobile carriers a) plastoquinone (PQ) analogous to UQ links PSII and the cytochrome bf complex b) plastocyanin in the lumen is a small protein linking cytochrome bf complex and PSI (so analogous to cytochrome c ) Slide 32 The source of energy is light. This energy is absorbed and transferred to the two reaction centres, which use the energy to make an electron in the chlorophyll special pair more reducing. PS II takes electrons from H 2 O ( E o 1/2 O 2 /H 2 O =+0.82V) and donates electrons to PQ(E o PQ/PQH 2 = 0V). Reaction centre has added 0.82V of reducing energy, using absorbed light energy to do so. Electrons then flow downhill in energy terms to plastocyanin (E 0 =+0.38V), where PSI uses light energy to take this electron and use it to reduce NADPH (E o = - 0.32V) via ferredoxin and ferredoxin-NADP + oxidoreductase (FNR). Light causes electrons to flow from H 2 O to NADPH. This also leads to generation of a proton-motive force and thus ATP synthesis (photophosphorylation Slide 33 PROTON PUMPS IN PHOTOSYNTHETIC ELECTRON TRANSFER Slide 34 There are three proton pumps 1) oxidation of water by PSII in the lumen releases protons 2) the cytochrome bf complex pumps protons from the stroma to the lumen 3) the reduction of NADP + in the stroma removes protons So a proton-motive force is created with the lumen the P side, the stroma the N side. Protons flow back from the lumen to the stroma via an ATP synthase similar to that found on the IMM, leading to ATP synthesis. Slide 35 Summary Takes place in chloroplasts Takes place in chloroplasts Light absorption by chlorophyll Induces electron transfer Light absorption by chlorophyll Induces electron transfer Two photosystems generate Proton Gradient and NADPH Two photosystems generate Proton Gradient and NADPH Proton gradient drives ATP synthesis Proton gradient drives ATP synthesis Slide 36 BENSON-CALVIN CYCLE (The dark reaction) ATP and NADPH formed in the light reactions of photosynthesis are used to convert CO 2 into hexose ( a 6 carbon sugar) and other carbon compounds. The dark reactions of photosynthesis start Slide 37 Calvin Cycle Slide 38 Catalysed by rubisco Slide 39 Slide 40 6C 3C 4C 5C Slide 41 Slide 42 Slide 43 1 The dark reactions of photosynthesis start with the reaction of CO 2 and ribulose 1,5-bisphosphate (5C) to form two molecules of 3-phosphoglycerate (3C). This reaction is catalysed by ribulose 1,5-bisphosphate carboxylase/oxygenase (usually called rubisco). This carboxylase is also an oxygenase, because oxygen can also react with the enzyme to form phosphoglycolate (2C) and 3-phosphoglycerate. The recycling of phosphoglycolate leads to the wasteful loss of organic C as CO 2 and the consumption of O 2, in a process known as photorespiration. Slide 44 ENERGY REQUIRED TO FIX CARBON IN THE CALVIN CYCLE Six rounds of the Calvin cycle are required to synthesise 1 hexose (6C) from 6 CO 2. So a balanced equation for the net reaction of the Calvin cycle is 6 CO 2 + 18 ATP + 12 NADPH + 12 H 2 O C 6 H 12 O 6 + 18 ADP + 18 P i + 12 NADP + + 6H + Slide 45 1,3-phosphoglycerate (3C) is converted into fructose 6-phosphate (6C) in four reactions which resemble similar steps in gluconeogenesis (the synthesis of glucose from non-carbohydrate sources such as lactate, amino acids and glycerol). Several of these reactions in the Calvin cycle and gluconeogenesis (synthesis of 6C) are shared with glycolysis (breakdown of 6C), except the essentially irreversible reactions in glycolysis have to be bypassed by new reactions. These steps in the Calvin cycle consume 2ATP and 2NADPH per 6C formed from 2(3C). The remaining task is to regenerate ribulose 1,5-bisphosphate (the CO 2 acceptor), in other words to construct a 5C sugar from 6C and 3C sugars, a rearrangement that consumes 1ATP Slide 46 STARCH AND SUCROSE ARE THE MAJOR CARBOHYDRATE STORES IN PLANTS Starch is a polymer of glucose residues. Starch is synthesized and stored in the chloroplasts. Sucrose is a dissacharide. Sucrose is synthesised in the cytosol, using triose phosphates (3C) exported from the chloroplasts. Slide 47 THIOREDOXIN CO-ORDINATES LIGHT AND DARK REACTIONS Carbon dioxide assimilation and other biosynthetic reactions are switched on in the light by reduced thioredoxin A 12-kd protein reduced by ferredoxin which is reduced in turn by PSI in the light. The reduced thioredoxin reduces disulphide bridges which control the activities of biosynthetic enzymes. Slide 48 Slide 49 Slide 50 Enzymes involved in carbohydrate degradation are switched off in the light when reduced thioredoxin reduces their disulphide bridges. The rate-limiting step in the Calvin cycle is that catalysed by rubisco. The activity of this enzyme increases in the light because the pH of the stroma increases from 7 to 8, and the level of Mg 2+ increases in the stroma, as H + are pumped into the lumen. Slide 51 Summary Calvin cycle synthesises hexoses from Carbon dioxide and water Calvin cycle synthesises hexoses from Carbon dioxide and water Activity of cycle depends on environmental conditions Activity of cycle depends on environmental conditions