Chemical Safety

Handbook

The University of Texas

Health Science Center

at San Antonio

February 2014

Revision 5

Table of Contents Page Number

1.0 Introduction …………………………………………………………………………….. 5

1.1 Purpose …………………………………………………………………………. 5 1.2 Regulations and Responsibilities under

the Texas Hazard Communication Act ….…………………………………… 6 1.3 Responsibilities ………………………………………………………………… 6 1.3 Chemical Safety Committee ………………………………….………………. 6 1.4 Chemical Hygiene Officer ……………………………………. ……………… 7 1.5 EH&S ……………………………………………………………………………. 7 1.6 Principal Investigator ………………………………………….. ……………… 7 1.7 Employee, Student, Volunteers.…………………………….……………….. 8 1.8 Reporting a Safety Concern ………………………………………………….. 8 1.9 Imminently Dangerous to Life & Health ……………………………………… 9 1.10 Observed Deficiencies and Escalated Notifications ……………………….. 9

2.0 Safety Data Sheets (SDS) ……………………………………………………………. 10 2.1 Definition ………………………………………………………………………… 10 2.2 Requirements …………………………………………………………………… 10 2.3 Components of an SDS ……………………………………………………….. 10 2.4 How to Obtain an SDS ………………………………………………………… 11

2.5 Requesting an SDS ……………………………………………………………. 13 3.0 Chemical Container Labeling ……………………………………………………….. 14

3.1 Requirements …………………………………………………………………… 14 3.2 Labeling of Secondary Containers …………………………………………... 15 3.3 Postings …………………………………………………………………………. 17

4.0 Fundamentals of Hazardous Chemicals …………………………………………… 19

4.1 Definitions ……………………………………………………………………….. 19 4.2 Health Hazards...……………………………………………………………….. 19 4.2.1 Toxicity ………………………………………………………………………….. 19 4.2.2 Routes of Exposure …………………………………………………… 19 4.2.3 Target Organ Toxicity ………………………………………………… 20 4.2.4 Dose ……………………………………………………………………. 20 4.2.5 Acute and Chronic Exposures ………………………………………. 20 4.2.6 Highly Toxic Chemicals ………………………………………………. 20 4.2.7 Toxic Chemicals ………………………………………………………. 21 4.2.8 Corrosive Chemicals ………………………………………………….. 21 4.2.9 Irritants …………………………………………………………………. 21 4.2.10 Sensitizers …………………………………………………………….. 21 4.2.11 Carcinogens ……………………………………………………. ……. 21 4.2.12 Ultraviolet Radiation ………………………………………………….. 22 4.3 Physical Hazards ………………………………………………………………. 23 4.3.1 Flammable Materials ………………………………………………….. 23 4.3.2 Combustible Liquids ………………………………………………….. 24 4.3.3 Compressed Gases ………………………………………………….. 24 4.3.4 Oxidizers ………………………………………………………………. 24 4.3.5 Explosives …………………………………………………………….. 24

4.3.6 Organic Peroxides ……………………………………………………. 24 4.3.7 Pyrophoric ……………………………………………………………. 24 4.3.8 Reactive Chemicals ………………………………………………….. 24 4.3.9 Water Reactive Chemicals …………………………………………… 25 4.4 Safe Handling …………………………………………………………............. 25 4.5 Storage and Segregation ……………………………………………………… 26 4.6 Transporting Hazardous Chemicals …………………………………………. 27

5.0 Compressed Gases and Liquid Nitrogen Safety ………………………………… 29 5.1 Background …………………………………………………………………….. 29 5.1.1 Compressed Gases …………………………………………………… 29 5.1.2 Liquid Nitrogen ………………………………………………………… 30 6.0 Evaluation of Special Chemical Hazards …………………………………………. 32

6.1 Background …………………………………………………………………….. 32 6.2 Special Hazards ……………………………………………………………….. 32 6.2.1 Carcinogens …………………………………………………………… 34 6.2.2 Asphyxiants ……………………………………………………………. 34 6.2.3 Narcotics ……………………………………………………………….. 34 6.2.4 Heavy Metals ………………………………………………………….. 34 6.2.5 Cyanides ………………………………………………………………. 34 6.2.6 Nerve Agents ………………………………………………………….. 34 6.3 Application for Approval to Work With Carcinogenic,

Highly Toxic, or Acutely Hazardous Chemicals ……………………………. 34 6.4 Chemical Carcinogen Lists …………………………………………………… 37

7.0 Monitoring of Hazardous Chemicals ………………………………………………. 38 7.1 Hazard Determination …………………………………………………………. 38 7.2 Recommendation for Chemical Exposure Assessments ………………….. 38 7.3 Performing Chemical Exposure Assessments ……………………............... 38 8.0 Hazardous Chemical Inventory ……………………………………………………… 39 8.1 Background ……………………………………………………………………… 39

8.2 New Principal Investigators …………………………………………………… 39 8.3 Annual Chemical Inventory Update ………………………………………….. 39 8.4 Common Use or Open Labs ………………………………………………….. 39 8.5 Record Keeping and Forms …………………………………………………… 39

9.0 Annual Laboratory Safety Evaluations ……………………………………………. 40 9.1 Background …………………………………………………………………….. 40

9.2 Laboratory Evaluation Record Report ………………………………………. 40 9.3 The Actual Audit and Follow-up Procedures ……………………………….. 41 9.4 Common Laboratory Deficiencies and Corrective Measures …………….. 41 9.5 Self-Assessment of Laboratories ……………………………………………. 42 9.5.1 Job Safety Hazard Analysis …………………………………………. 43 9.6 New PI Laboratory Risk Assessment ……………………………………….. 43

10.0 Laboratory Hygiene & Personal Protective Equipment ……………………….. 44 10.1 Personal Hygiene, Habits, and Practices …………………………………… 44

10.2 Laboratory Housekeeping ………………………………..…………………… 45

10.3 Availability of Personal Protective Equipment (PPE) ……………………… 45 10.4 Training for Personal Protective Equipment (PPE)...………………………. 46 10.5 UTHSCSA Policy on Wearing Gloves ………………………………………. 46

11.0 Laboratory Ventilation and Chemical Fume Hoods ……………………………. 49 11.1 Background …………………………………………………………………….. 49 11.2 General Laboratory Ventilation ………………………………………………. 49 11.3 Chemical Fume Hoods ……………………………………………………….. 49 11.3.1 Types of Chemical Fume Hoods ……………………………………. 49 11.3.2 Hood Components ……………………………………………………. 51 11.4 Fume Hood Schematics ……………………………………………………… 52 11.5 Fume Hood Work Practices ………………………………………………….. 52 11.6 Annual Testing Requirements ……………………………………………….. 54 12.0 Shipping of Hazardous Chemicals ………………………………………………… 55 12.1 Background …………………………………………………………………….. 55 12.2 Hazard Classifications and Divisions ………………………………………… 55 12.3 Training Requirements ………………………………………………………… 56 12.4 Record Keeping ………………………………………………………………… 56 13.0 Training …………………………………………………………………………………. 57 13.1 Hazard Communication ……………………………………………………….. 57

13.2 Laboratory Safety & Hazardous Waste Generator’s Training…………….. 57 13.3 Site-Specific Training.…………………………………………………………. 58 13.4 Incidental Training ……………………………………………………………… 58 13.5 Record Keeping ………………………………………………………………… 58

14.0 Hazardous Chemical Waste Management ………………………………………… 59 14.1 Responsibilities …………………………………………………………………. 59

14.2 Legislative Background and Requirements …………………………………. 59 14.3 Definition of Hazardous Waste ……………………………………………….. 59 14.3.1 Pharmaceutical Waste ……………………………………………….. 60 14.3.2 Controlled Substances ……………………………………………….. 60 14.3.3 Chemotherapy or Antineoplastic Drugs …………………………….. 61 14.4 Disposal Methods ………………………………………………………………. 61 14.4.1 Sanitary Sewer …………………………………………………………. 61 14.4.2 Ordinary Trash ……………… …………………………………………. 61 14.4.3 Selection of Waste Containers and Packaging …………………….. 61 14.4.4 Labeling Hazardous Waste Containers ……….…………………….. 62 14.4.5 Campus Collection System ……………..…………………………….. 63 14.4.6 Filling Out a Chemical Waste Pickup Request ……………………... 64 14.5 Source Reduction / Recycling Hazardous Materials ……………….. ……… 65 14.6 Chemical Exchange Program …………………………………………………. 65

15.0 Chemical Spills and Emergency Response ……………………………………… 68 15.1 Emergency Plans ……………………………………………………………… 68 15.2 Reporting a Chemical Spill …………………………………………………… 68

15.2.1 Chemical Spill Response and Decontamination Procedures …… 68 15.3 Post Exposure/Injury …………………………………………………………… 69 15.4 Recommended First Aid Procedures …………………………………………. 69 15.5 Fire Prevention Procedures ……………………………………………………. 70

15.5.1 Responding to Fire Emergencies ……………………………………. 71 15.5.2 How to Extinguish Clothing Fires ……………………………………. 71

16.0 Decommissioning Laboratories …………………………………………………….. 72 16.1 Purpose ………………………………………………………………………….. 72

16.2 Responsibilities …………………………………………………………………. 72 16.2.1 Department Chair ………………………………………………………. 72 16.2.2 Principal Investigator ………………………………………..…………. 72 16.2.3 Environmental Health & Safety ……………………………………….. 72 16.3 Laboratory Decommissioning Procedure …………………………………….. 72

17.0 Recordkeeping …………………………………………………………………………. 75 17.1 Record Keeping Requirements for Laboratories ……………………………. 75 17.1.1 Chemical Safety Handbook …………………………………………… 75 17.1.2 Safety Data Sheets (SDS) ……………………………………………. 75 17.1.3 Laboratory Safety Evaluations ……………………………………….. 75 17.1.4 Employee Training …………………………………………………….. 75 17.1.5 Chemical Exposure Assessments …………………………………….75 17.2 Record Keeping Requirements for Environmental Health & Safety Office 75 17.2.1 Hazard Communication Program …………………………………….. 75 17.2.2 Chemical Safety Handbooks …………………………………………..75 17.2.3 Tier Two Report ………………………………………………………….75 17.2.4 Safety Data Sheets (SDS) ……………………………………………...75 17.2.5 Chemical Inventories ………………………………………………….. 75 17.2.6 Laboratory Safety Evaluations ……………………………………….. 75 17.2.7 Employee Training …………………………………………………….. 76 17.2.8 Chemical Exposure Assessments …………………………………… 76 17.2.9 Incident Reports ……………………………………………………….. 76 17.2.10 Records of Terminated Principal Investigators ……………………. 76 Appendices: A TDSHS Notice to Employees B Chemical Carcinogens Regulated by Occupational Safety and Health Administration (OSHA) C Substances Known to Be Human Carcinogens as Listed by the National Toxicology Program (NTP) D Group 1 (Known Human Carcinogens), Group 2A (Probably Carcinogenic to Humans) as Evaluated in the International Agency for Research on Cancer (IARC) Monographs, volumes 1-88 E EPA “P” Listed Acutely Hazardous Waste F EPA “U” Listed Toxic Hazardous Waste G Toxic Characteristic Constituents and Regulatory Levels H Principal Investigator’s/Supervisor’s with Hazardous Chemicals Memo I Laboratory Safety Evaluation Record J Site-Specific Chemical Hazard Training Form K Laboratory Decommissioning Checklist

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1.0 Introduction

1.1 Purpose. The purpose of the University of Texas Health Science Center’s Chemical Safety Handbook is to provide a written document which complies with the regulations set forth by the Texas Hazard Communication Act of 1986, specifically, to reduce the incidence of chemically-related occupational illnesses and injuries and to provide a means for the general public to learn about the chemical hazards associated with businesses in the community. The Chemical Hygiene Plan intends to comply with the Occupational Safety & Health Administration (OSHA), regulatory standard contained in 29CFR1910.1450. The objective of this Chemical Safety Handbook is to serve as the Written Hazard Communication Plan and Chemical Hygiene Plan for UTHSCSA as a means for providing safety guidelines to those employees who work with or are exposed to hazardous chemicals in the routine course of their job duties. This handbook provides general information to employees and students in the use and handling of hazardous chemicals. Topics addressed include information on Safety Data Sheets (SDS), container labeling requirements, safe handling, use, storage and disposal of hazardous chemicals, and employee training programs. Additionally, this handbook provides definitions of hazardous chemicals, factors affecting chemical action on the body, emergency procedures, proper personal protective equipment, first aid, and employee rights and responsibilities under the Texas Hazard Communication Act. The Chemical Safety Handbook is not intended to be an extensive or fully comprehensive manual, but rather a guide for knowledgeable faculty, staff, and students. Additional information regarding hazards associated with specific hazardous chemicals should be obtained through consultation with the Chemical Safety Committee and Environmental Health & Safety’s Chemical Safety Division. All faculty and staff with exposure to hazardous chemicals must be familiar with the requirements set forth in this manual and comply with the rules under the Texas Hazard Communication Act (THCA) enforced by the Texas Department of State Health Services. All occupational operations conducted in UTHSCSA facilities must be performed in accordance with the applicable requirements of this handbook. Signature on file Signature on file Michael A. Charlton, Ph.D. David Wampler, Ph.D. Assistant Vice President for Risk Chair, Chemical Safety Committee Management and Safety The University of Texas Health Science The University of Texas Health Science Center at San Antonio Center at San Antonio Signature on file Signature on file James D. Kazen William Henrich, M.D., MACP Executive Vice President President Facility Planning and Operations The University of Texas Health Science The University of Texas Health Science Center at San Antonio Center at San Antonio

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1.2 Regulations and Responsibilities under the Texas Hazard Communication Act. The Texas Hazard Communication Act originally became effective on January 1, 1986 and was revised in 1993. The purpose of this act is to reduce the incidence of chemically related occupational illness and injury and to provide a means for the general public to learn about the chemical hazards associated with businesses in the community. The Act sets the minimum requirements employers must meet for providing information about hazardous chemicals in the workplace to employees and other interested parties. It is patterned after the federal Occupational Safety and Health Administration (OSHA) Hazard Communication Standard and is enforced by the Texas Department of State Health Services. Under the Act, the University of Texas Health Science Center (UTHSCSA) must notify employees of their rights under the act, compile workplace chemical lists for the non-research areas, train all exposed employees regarding the hazards associated with the chemicals the use, maintain a file of l Safety Data Sheets (SDS), and supply the appropriate emergency response personnel with information. The Hazard Communication Act gives employees certain rights. Employees who may be or may have been exposed to hazardous materials in the workplace under normal conditions or foreseeable emergencies are covered by the Act. All students are considered to be employees under this definition. Employee rights are listed in the “Notice to Employees” found in Appendix A. They include:

1. The right to be informed of exposures to hazardous chemicals. 2. The right to access to Safety Data Sheets and other references regarding chemical

hazards. 3. The right to receive training on the hazards of the chemicals and on measures which can

be taken to protect them from these hazards. 4. Employees may not be required to work from unlabeled containers of hazardous

chemicals, except for portable containers for immediate use, the contents of which are known to the user.

5. Employees may file complaints with the Texas Department of State Health Services and may not be discharged or discriminated against in any manner for the exercise of any rights provided by this Act.

1.3 Responsibilities- Chemical Safety Committee. The Chemical Safety Committee is a body consisting

of peers appointed to serve in an advisory and consultative capacity to the President and the Vice President for Research, and to work with the Environmental Health and Safety Department in matters pertaining to: a) the safe receipt, use, storage, and disposal of hazardous chemicals

b) review of safety standards involving chemicals prior to promulgation and execution

c) review of plans for chemical safety programs (those required and/or those not required by law or by granting/contracting agency) in order to determine their applicability to the research/training function of the Health Science Center; in matters pertaining to the safe receipt, use, storage, and disposal of hazardous chemicals

d) serve as an avenue of appeal in cases of dispute and exception.

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1.4 Responsibilities- Chemical Hygiene Officer. The Chemical Hygiene Officer is an employee who is designated by the employer, and who is qualified by training or experience, to provide technical guidance in the development and implementation of the provisions of the Chemical Hygiene Plan. In accordance with OSHA standards, the Chemical Hygiene Officer will be the Director of Environmental Health and Safety Department, or qualified staff member so appointed by the Director. Under the Texas Hazard Communication Act, the University of Texas Health Science Center at San Antonio (UTHSCSA) must:

a) notify employees of their rights under the act

b) compile workplace chemical lists for the non-research areas

c) train all exposed employees regarding the hazards associated with the chemicals in use

d) maintain an accurate electronic database of Safety Data Sheets (SDS)

e) supply the appropriate emergency response personnel with information 1.5 Responsibilities- Environmental Health & Safety. The Environmental Health & Safety Department will:

a) maintain the Chemical Safety Handbook for the use of hazardous chemicals at UTHSCSA with the advice and guidance of the Chemical Safety Committee

b) provide consultation and technical information for handling hazardous chemicals

c) review proposed research protocols for the use of hazardous chemicals, and submit these to the Chemical Safety Committee with recommendations

d) provide training in chemical safety and other specialized training as requested

e) perform injury/accident investigations related to potential chemical exposures

f) perform laboratory safety evaluations

g) conduct emergency response for follow-up on spills of hazardous chemicals within the

scope of their response training on UTHSCSA property

h) interact with outside emergency response organizations and regulatory agencies 1.6 Responsibilities- Principal Investigator. The principal investigator (PI) is the individual who submits the application to employ hazardous chemicals in his or her work or is the responsible faculty supervisor over student labs. This individual has the primary responsibility for:

a) adhering to all guidelines, policies and regulations

b) the safe use of hazardous chemicals by himself/herself and those under his or her direction

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c) complying with and enforcing all UTHSCSA standards, policies, procedures and

referenced regulations regarding the use, handling, containment, and disposal of all hazardous chemicals as outlined in this handbook

d) ensuring that staff are aware of and trained in the hazards, safe handling and disposal of

hazardous chemicals

e) ensuring that staff are supplied with the appropriate personal protective equipment (PPE)

f) ensuring that safety equipment is available in the laboratory and used when necessary g) completing necessary documentation to report potential exposures

h) reporting all hazardous material spills as soon as possible to Environmental Health &

Safety

1.7 Responsibilities- Individual Employees, Students, Volunteers. It is the responsibility of individual employees, students and volunteers to:

a) comply with established safety procedures and policies

b) maintain awareness of the potential risks associated with assigned duties

c) attend all required training classes to stay in compliance with relevant rules and regulations

d) take all necessary safety precautions pertaining to their job duties

e) wear the appropriate personal protective equipment at all times when at risk for injury

f) inform their immediate supervisor or EH&S of any unsafe practices or conditions

g) inform their immediate supervisor of any injuries or exposures

h) report to their immediate supervisor of any change in their health status if there is a

possibility it may be work-related

i) report all chemical spills and accidents to their supervisor

1.8 Reporting a Safety Concern. All safety concerns should be reported to Environmental Health & Safety (1.343T DTL, 567-2955). However, all employees, students and volunteers are encouraged to talk to their immediate supervisors regarding unsafe conditions. In the event that the supervisor is unable to resolve the issue, Environmental Health & Safety can offer assistance.

Employees may also file complaints regarding an alleged violation of rights under the Texas Hazard Communication Act with the Texas Department of State Health Services at 1-888-963-7111.

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The Occupational Safety and Health Act of 1970 give employees the right to file complaints about workplace safety and health hazards. The Act also gives complainants the right to request that their names not be revealed to their employers. If you would like to report a work-related safety violation to OSHA, please refer to the website, http://www.osha.gov/as/opa/worker/complain.html.

1.9 Imminently Dangerous to Life and Health. If Environmental Health & Safety notes any conditions where there is risk of imminent danger to life, health, or facilities, this will be brought to the immediate attention of the appropriate Safety Manager(s) and the Director of Environmental Health & Safety. Corrective action may include immediate shut down of all operations as outlined in the January 1, 1995 memorandum from the UTHSCSA President titled, Responsibilities and Authority of the Institutional Safety Officer.

1.10 Observed Deficiencies and Escalated Notifications. Any noted deficiencies observed

during routine laboratory evaluations will be corrected by the responsible principal investigator (PI) within the required time frame (usually 30 business days). Deficiencies posing an unusual hazard, or those of a serious nature that have not been resolved after a 60 day period, will advance to an Escalated Deficiency. Environmental Health & Safety will use the following notification methods and time line:

a) A letter from the Chemical Safety Manager to the PI/Supervisor – 30 business days to

respond b) A letter from the appropriate Safety Committee Chair to PI/Supervisor – 30 business days

to respond c) A letter from the Director of Environmental Health & Safety to the Department Chair – 30

business days to respond d) A letter from the Director of Environmental Health & Safety to the Dean of supervising

school – 30 business days to respond e) A letter from the Director of Environmental Health & Safety to the Executive Vice President

– 30 business days to respond f) A letter to the UTHSCSA President – 30 business days to respond g) A letter to the UT Board of Regents – 30 business days to respond

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2.0 Safety Data Sheets (SDS) 2.1 Definition. Safety Data Sheets (SDS) are documents containing hazard and safe handling information that is prepared in accordance with the requirements of the federal Occupational Safety and Health Administration (OSHA) standard for that document. The Hazard Communication Standard (HCS) requires chemical manufacturers, distributors, or importers to provide Safety Data Sheets (SDSs) (formerly known as Material Safety Data Sheets or MSDSs) to communicate the hazards of hazardous chemical products. 2.2 Requirements. A current SDS must be readily available for each hazardous chemical in the work area. All employees receive training during New Employee Orientation regarding an understanding of safety information found in SDSs, as well as ways to access SDSs. Each clinic or laboratory is then responsible for ensuring that all personnel have access to a paper or electronic copy of SDS for all hazardous chemicals used and stored in their immediate area during all work shifts. Environmental Health & Safety recommends that each laboratory review the current SDS each year during the chemical inventory process. 2.3 Components of an SDS. As of June 1, 2015, the HCS will require new SDSs to be in a uniform format, and include the section numbers, the headings, and associated information under the headings described below: Section 1: Identification - includes product identifier; manufacturer or distributor name, address, phone number; emergency phone number; recommended use; restrictions on use.

Section 2: Hazard(s) Identification - includes all hazards regarding the chemical; required label elements.

Section 3: Composition/Information on Ingredients - includes information on chemical ingredients; trade secret claims.

Section 4: First-aid Measures - includes important symptoms/ effects, acute, delayed; required treatment.

Section 5: Fire-fighting Measures - lists suitable extinguishing techniques, equipment; chemical hazards from fire.

Section 6: Accidental Release Measures - lists emergency procedures; protective equipment; proper methods of containment and cleanup.

Section 7: Handling and Storage - lists precautions for safe handling and storage, including incompatibilities.

Section 8: Exposure Controls/Personal Protection - lists OSHA's Permissible Exposure Limits (PELs); Threshold Limit Values (TLVs); appropriate engineering controls; personal protective equipment (PPE).

Section 9: Physical and Chemical Properties - lists the chemical's characteristics.

Section 10: Stability and Reactivity - lists chemical stability and possibility of hazardous reactions.

Section 11: Toxicological Information - includes routes of exposure; related symptoms, acute and chronic effects; numerical measures of toxicity.

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Section 12: Ecological Information*

Section 13: Disposal Considerations*

Section 14: Transport Information*

Section 15: Regulatory Information*

Section 16: Other information - includes the date of preparation or last revision.

*Note: Since other Agencies regulate this information in these sections, OSHA does not enforce Sections 12 through 15(29 CFR 1910.1200(g)(2)). 2.4 How to Obtain an SDS. There are several sources for obtaining SDS here at UTHSCSA:

a) SDS Binders – Supervisors may opt to keep binders with current SDS for every hazardous chemical used in their immediate area. Any work area that does not have computer access is required to keep paper copies of all current SDS.

b) Environmental Health & Safety Office - maintains an electronic database of current SDS. Whenever a new hazardous chemical is ordered or whenever a SDS is updated, the SDS is forwarded to the Environmental Health & Safety Office.

c) Internet or Web Access – SDS may be accessed through the internet (Google, manufacturer websites). Additionally, the Environmental Health & Safety Office offers a link to a database entitled SDSpro, which provides a user-friendly way to access SDS.

1. Click on the SDSpro icon from Environmental Health & Safety website, under Quick Links, http://research.uthscsa.edu/safety.

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2. Type in the name of the chemical in the square located on the upper left corner of the screen, and click on the Search button:

3: Click on the Product Name/Synonym to select the SDS of interest. You can print a copy for your records or save an electronic copy:

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2.5 Requesting an SDS. To request an SDS for a specific chemical that is not located in the database, click on the highlighted statement (as seen below) and fill in all boxes and click on the “Submit” button.

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3.0 Chemical Container Labeling and Postings 3.1 Requirements. The Occupational Safety & Health Administration (OSHA) has updated the requirements for labeling of hazardous chemicals under its Hazard Communication Standard (HCS). As of June 1, 2015, all manufacturer labels will be required to have the product identifier, signal word, hazard and precautionary statements, supplier identification, and pictograms. The labels on these primary containers should not be defaced or removed. If, for any reason, these labels are removed or become illegible, the containers must be re-labeled. In addition, chemicals received at UTHSCSA must be clearly marked with “Date Received” and “Date Opened”. Below is an illustration that identifies the required label elements, under the new OSHA standard.

Figure 3-1: Example of a chemical container label

A. B.

Figure 3-2: Manufacturer label from Sigma Aldrich A. New GHS pictograms B. Received date and opened date written on label

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Figure 3-3: GHS Pictograms 3.2 Labeling of Secondary Containers. When transfers are made from stock bottles to secondary containers, these secondary containers must be properly labeled. Examples of secondary containers include but are not limited to glass or plastic bottles, squirt bottles, test tubes, Erlenmeyer flasks, and beakers. It is important to note here that all containers must be properly

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labeled even if they do not containing hazardous material. Secondary containers which have greater that 1% of a hazardous chemical or greater that 0.1% of a highly toxic or acutely hazardous chemical must be labeled as follows:

a) Chemical name b) Hazard identification (written or visual format) c) Date received/prepared

The hazard warning may be displayed in written or visual format. All labels must be written in English as the primary language. The Environmental Health & Safety Office provides labels that contain the new pictograms for labeling secondary containers. Similar labels may be purchased through different vendors. Below are examples of acceptable labels for secondary containers:

Figure 3-4: Glass Corning bottles labeled with Figure 3-5: Secondary Label - Health secondary Label Hazard Pictogram

Figure 3-6: Pre-labeled wash bottle Figure 3-7: Example of label in written format

Acetone

WARNING!Liquid causes eye burns

Avoid contact with skin & eyes

FLAMMABLEKeep away from heat, sparks & open flames

First Aid: In case of contact, flush skin and eyes with water for 15 minutes

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3.3 Postings. All laboratories at UT Health Science Center must display a laboratory hazard placard at the lab’s entrance. This placard will identify any chemical, biological, radiological, or other types of hazards that are present in the immediate area. Entry and exit requirements, such as personal protective equipment requirements, or medical surveillance requirements will also be displayed. Finally, emergency contact information will include primary and backup name for the lab, including a daytime and evening phone number. The UTHSCSA emergency procedures for evacuation of our buildings are also included on the placard. In the event of an emergency, you can contact UT Police by dialing 911 from any campus phone, or 210-567-8911 from a cell phone.

Figure 3-6: Hazard Warning(s) Posting at Laboratory Entrance

Emergency contacts Entry Exit Requirements Medical Surveillance Requirements Biosafety Level & agents in use

GHS Pictograms (Chemical Hazards) No Eating or Drinking and other hazard symbols Emergency contacts RACE acronym

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Figure 3-7: Notice to Employees, Texas Department of State Health Services

NO FOOD or DRINK TO BE STORED IN THIS REFRIGERATOR or FREEZER

Figure 3-8: No Food or Drink Sign for Refrigerators, Freezers

Figure 3-9: Equipment Clearance Posting

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4.0 Fundamentals of Hazardous Chemicals 4.1 Definitions. On UTHSCSA campuses, laboratory personnel work with a wide variety of chemicals. The hazard presented by a chemical is dependent on both its physical and toxicological properties. Many chemicals exhibit multiple hazards. A hazardous chemical is defined as an element, compound, or mixture of elements or compounds that has a physical hazard or health hazard as defined by the OSHA standard in 29 CFR Section 1910.1200 (c), or a hazardous substance as defined by the OSHA standard in 29 CFR Section 1910.1200 (d) (3), or by OSHA’s written interpretations. 4.2 Health Hazards. Chemicals which can cause damage, reversible or irreversible, to the human body are considered to be health hazards. Health hazards are defined as those chemicals for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees. The term “health hazard” includes chemicals which are carcinogens, toxic or highly toxic agents, reproductive toxins, irritants, corrosives, sensitizers, hepatotoxins, nephrotoxins, and neurotoxins, agents which act on the hematopoietic system and agents which damage the lungs, skin, eyes, or mucous membranes. These categories are discussed below.

4.2.1 Toxicity. Toxicology is the study of the adverse effects of chemicals on living organisms. The toxicity of a specific substance depends on a number of factors. The primary ones are as follows:

• The quantity of material involved.

• The rate and extent to which a chemical is absorbed into the bloodstream.

• The rate and extent to which the chemical is metabolized and whether or not the

metabolites are toxic.

• The rate and extent to which the chemical and its metabolites are removed from the body.

Other factors also influence the effect that a given chemical has on the body. How the chemical enters the body, the amount of the chemical in the body, and the acute and/or chronic toxicity of the chemical contribute to its hazard. The major routes of chemical access to the body are the lungs (inhalation), the skin (topical absorption), and the gastrointestinal tract (ingestion).

4.2.2 Routes of Exposure. Inhalation is by far the most important route of chemical entry.

Any chemical that becomes airborne can be inhaled either as a dust or a vapor. For a given chemical the total quantity absorbed through the respiratory tract depends on its concentration in the air, the duration and frequency of exposure, and the rate of breathing.

The skin is not highly permeable to most chemicals and provides a relatively good barrier to protect from toxics in the environment. However, some chemicals can be absorbed through the skin in sufficient quantities to produce systemic effects. An example is carbon tetrachloride. The health of the skin also influences chemical penetration. Skin that is diseased or abraded offers direct access into the body. The absorption of toxic materials through the skin varies

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under a number of circumstances. Contact with water increases the hydration of the skin and increases its permeability two-three fold. In addition various chemicals such as dimethyl sulfoxide (DMSO) facilitate the penetration of toxicants through the skin.

The gastrointestinal tract is rarely a route for occupational poisoning. However, accidental ingestions have occurred. Swallowing of particles cleared by the respiratory tract can contribute to the route of poisoning.

4.2.3 Target Organ Toxicity. Metabolic and excretory processes work to keep the body free of foreign substances. The liver and kidney help protect the body against poisoning and aid in removing poisonous substances. However, some toxic substances are accumulated in these same organs. Hepatotoxins, such as carbon tetrachloride act principally to damage the liver. Nephrotoxins, including halogenated hydrocarbons and uranium, cause damage to the kidneys.

As the bloodstream circulates toxic chemicals throughout the body, every organ is in contact with the material. Many poisons show a selective affinity for the cells of a particular organ and produce specific effects on them. Poisons affecting the nervous system are called neurotoxins. An example of a neurotoxin is tetrodotoxin. Those affecting the circulatory system are hemotoxins, such as snake venom. Reproductive toxins such as toluene are agents which interfere with normal reproductive capabilities. Mutagens such as ethidium bromide are substances which cause genetic damage. Chemicals which cause defects of fetal development are called teratogens. Examples of teratogens include lead and ethylene oxide.

4.2.4 Dose. The amount of chemical which enters the body also influences the effect. To help quantitate the relationship between dose and response and to provide guidelines for use, the American Conference of Governmental Hygienists (ACGIH) publishes a book of Threshold Limit Values (TVL) for many common industrial chemicals. These TLVs are exposure levels to which it is felt a healthy working population can be exposed for forty hours a week with no ill effects. OSHA’s Permissible Limits (PELs) are based on the TLVs. Chemical fume hoods should be provided in laboratories where work is being done with volatile chemicals having a TLV less than 50 ppm. Additional information regarding worker exposure levels is outlined in Chapter 7 of this handbook.

4.2.5 Acute and Chronic Exposures. Expressions of toxicity can be divided into those having acute and those having chronic effects. Acute exposures involve short-term (usually high) concentrations resulting in illness, irritation, or death. Chronic effects are characterized by symptoms or disease following frequent exposure over a long time period. Symptoms of chronic poisoning are frequently different from those seen in acute poisoning by the same agent. For example, acute poisoning by benzene results in damage to the central nervous system, while chronic exposure affects blood cell production capacity of the bone marrow.

4.2.6 Highly Toxic Chemicals. A chemical is considered highly toxic if it falls into any of the following categories:

• A chemical which has a LD50 (dose at which 50% of the test animals die) of 50 mg

or less per kilogram of body weight when administered orally in albino rats.

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• A chemical which has an LD50 of 200 mg or less per kilogram of body weight when administered by continuous contact for 24 hours (or less if death occurs) with the bare skin of albino rabbits.

• A chemical which has an LC50 (the concentration in air at which 50% of the test

animals die) of 200 parts per million (ppm) by volume or less of gas or vapor, or 2 mg per liter or less if mist, fume, or dust, when administered by continuous inhalation for one hour (or less if death occurs) in albino rats.

4.2.7 Toxic Chemicals. A chemical is considered toxic if it falls into any of the categories below:

• A chemical with an LD50 between 50 mg and 500 mg per kilogram body weight when administered orally to albino rats.

• A chemical with an LD50 between 200 mg and 1000 mg per kilogram body weight

when administered by continuous contact with the skin of albino rats for 24 hours.

• A chemical with an LC50 in air between 200 and 2000 ppm by volume, or between 2mg and 20 mg per liter of dust, mist or fume when administered by inhalation for one hour to albino rats.

4.2.8 Corrosive Chemicals. A chemical is defined as corrosive if it causes visible destruction of or irreversible alteration in, living tissue by chemical action at the site of contact. For example, a chemical is corrosive if, when tested on the skin of albino rabbits, it destroys or changes irreversibly the structure of the tissue at the site of contact following an exposure period of four hours. Examples are shown in Table 4-1.

4.2.9 Irritants. A chemical which causes a reversible inflammatory effect on living tissue particularly the skin, eyes, nose or respiratory system is an irritant. Examples are shown in Table 4-1.

4.2.10 Sensitizers. A sensitizer is a material which causes an allergic reaction of the skin or respiratory system. Examples are shown in Table 4-1.

4.2.11 Carcinogens. Chemical carcinogens are defined as substances with the ability to cause tumors. A chemical is considered to be a carcinogen under the Hazard Communication Act if:

• It has been evaluated by the International Agency for Research on Cancer (IARC),

and found to be a carcinogen or potential carcinogen. • It is listed as a carcinogen or potential carcinogen in the Annual Report on

Carcinogens published by the National Toxicology Program (NTP).

• It is regulated by OSHA as a carcinogen.

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These lists are found in Appendix B, C, and D. In medical research laboratories experimental chemicals and drugs may be used which are not on these lists but are known to induce mutations and tumors in experimental animals and other hosts. These materials should be treated as carcinogens as appropriate. Contact Environmental Health & Safety for help in determining safe handling and disposal practices. 4.2.12 Ultraviolet Radiation. Ultraviolet radiation can be used by the lab worker for many purposes. UV lamps or bulbs are apparent in many biological safety cabinets and tissue culture rooms as a means to provide some germicidal effects. Lasers found in some research equipment may also have a UV source. Exposure to ultraviolet radiation can present many hazards to the lab worker, such as dermal burns and retinal damage. The following preventative measures will minimize accidental exposure to UV radiation:

1. Always turn off UV lights prior to entering tissue culture rooms. 2. Always turn off UV lights prior to working at biological safety cabinets. 3. Wear appropriate protective equipment such as UV rated safety goggles.

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Table 4-1 below shows some common chemicals that are health hazards:

Health Hazards Examples Toxic – cause damage to living tissue. Toxicity falls into the following categories:

LD50 between 50 mg and 500 mg per kilogram of body weight.

LD50 between 200 mg and 1000 mg per kilogram body weight.

LC50 in air between 200 and 2000 ppm by volume, or between 2 mg and 20 mg per liter of dust, mist or fumes.

2-mercapto-ethanol Trypan Blue

Lead Compounds

Corrosive – defined as a chemical that causes visible destruction of, or irreversible alteration in living tissue by chemical action at the site of contact.

Acids Bleach Phenol

Irritants – cause a reversible inflammatory effect on living tissue particularly the skin, eyes, nose, or respiratory tract.

Bleach 10% formalin

Jet Acrylic Sensitizers – defined as a material which causes an allergic reaction of the skin or respiratory tract.

Latex rubber Nickel

Chromium Carcinogens – defined as substances with the ability to cause tumors. A chemical is considered to be a carcinogen if it has been evaluated by the International Agency for Research on Cancer (IARC), and found to be a carcinogen or potential carcinogen; it is listed as a carcinogen or potential carcinogen in the Annual Report on Carcinogens published by the National Toxicology Program (NTP); it is regulated by OSHA as a carcinogen.

Benzene

Chloroform Methylene Chloride

Formaldehyde Acrylamide

Ethidium Bromide

4.3 Physical Hazards. Physical hazards are defined as those chemicals for which there is scientifically valid evidence that it is a combustible liquid, a flammable, compressed gas, explosive, an organic peroxide, oxidizer, pyrophoric, an unstable (reactive), or water-reactive.

4.3.1 Flammable Materials. Liquids with a flashpoint below 100oF (30

oC), solids which burn

vigorously when ignited, gases which form flammable mixtures with air, and aerosols which will form a flame projecting from a valve in the presence of an ignition source are all defined as flammable materials under the Hazard Communication Act. The flash point of a liquid is the temperature at which sufficient vapors are given off by the liquid to form an ignitable mixture with air. Many common solvents have flash points well below room temperature. Maximum quantities of flammable and combustible liquids are outlined per NFPA 45, Standard on Fire Protection for Laboratories Using Chemicals (2011 edition). A summary of Table 10.1.1(a) from NFPA 45 is below:

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Table 4-2: Maximum Amounts of Flammable and Combustible Liquids for Laboratories

Quantities in Use Quantities in Use and Storage

Maximum Quantity /9.3m2 of lab unit (Liters)

Maximum Quantity /lab unit (Liters)

Maximum Quantity /9.3m2 of lab unit (Liters)

Maximum Quantity /lab unit (Liters)

Laboratory Unit Fire Hazard Class

Flammable and Combustible Liquid Class

A (high hazard)

I I,II, and IIIA

38

76

1820

3028

76

150

1820

6060 B a (moderate hazard)

I I, II, and IIIA

20

38

1136

1515

38

76

1820

3028 C b (low hazard)

I I, II, and IIIA

7.5

15

570

757

15

30

1136

1515 D b (minimal fire hazard)

I I, II, and IIIA

4 4

284

284

7.5

7.5

570

570

a Quantities are reduced by 50% for Class B labs located above the 3rd floor b Quantities are reduced by 25% for Class C and D labs located between 4th – 6th floors and reduced by 50% for Class C and D labs located above the 6th floor 4.3.2 Combustible Liquids. Liquids with a flash point between 100

oF (38

oC) and 200

oF

(93oC) are defined as combustible liquids.

4.3.3 Compressed Gases. A compressed gas is defined as a gas or mixture of gases which has a pressure exceeding 40 psi (pounds per square inch) at 70

oF.

4.3.4 Oxidizers. Oxidizers cause or promote fire in organic materials either by themselves or by releasing oxygen. In addition, strong oxidizers are also corrosives. 4.3.5 Explosives. A chemical which causes a sudden, almost instantaneous release of gas, pressure, and heat when subjected to sudden shock, pressure or high temperature is considered to be an explosive. 4.3.6 Organic Peroxide Formers. Organic peroxide formers are chemicals which contain an oxygen-oxygen bond; these chemicals are strong oxidizers and may be explosive. Benzoyl peroxide is an example of an organic peroxide former. In addition, many common laboratory compounds may deteriorate becoming contaminated with peroxides over time. A common example is ether.

4.3.7 Pyrophorics. Pyrophoric materials are ones which ignite spontaneously on contact with air. Examples include butylithium, tributylaluminum, white phosphorus, and magnesium amide.

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4.3.8 Reactive Chemicals. Reactive, or unstable, chemicals are materials which will polymerize, decompose (but not explosively), or condense under conditions of temperature, shock or pressure. 4.3.9 Water Reactive Chemicals. Water reactive chemicals react with water to release a gas that is either flammable or a health hazard. Lithium and sodium are examples of water reactive chemicals.

Table 4-3 below shows some commonly-used chemicals which have physical hazards:

Physical Hazards Examples Flammables – liquids with a flash point below 100oF (38oC), solids which burn vigorously when ignited, gases which form flammable mixtures with air, and aerosols which will form a flame projecting from a valve in the presence of an ignition source.

Ethanol Acetone

Cyclohexane Methanol

Compressed gases – a gas or a mixture of gases which have a pressure exceeding 40 psi (pounds per square inch) at 70oF.

Nitrogen Carbon Dioxide

Oxygen Organic peroxides – chemicals that contain an oxygen-oxygen bond; are strong oxidizers and may become explosive.

Diethyl Ether Benzoyl Peroxide

Pyrophorics – chemicals that ignite spontaneously on contact with air.

Barium alloys Phosphorus

Titanium Trichloride Water-reactive – react with water to release a gas that is either flammable or a health hazard.

Lithium Sodium

Magnesium Metal Combustible liquids – liquids with a flash point between 100oF and 200oF (38oC and 93oC).

Acetic Acid Acetic Anhydride Isoamyl Alcohol

Explosives – chemicals that cause a sudden, almost instantaneous release of pressure, gas and heat when subjected to sudden shock, pressure or high temperature.

Ammonium Perchlorate Picric Acid

Unstable (reactive) – will polymerize, decompose (but not explosively) or condense under conditions of temperature, shock, or pressure.

Methyl Methacrylate

4.4 Safe Handling. All hazardous materials should be handled in the safest manner possible. Below are procedures for handling various types of hazardous chemicals: 1. Always carry large bottles with one hand securing the bottom of the bottle. 2. When weighing out hazardous chemicals in dry form, always work away from any drafts and wear the appropriate personal protective equipment (gloves, lab coat, mask, safety goggles, etc.). 4. When pouring volatile organic chemicals, always work in a chemical fume hood and wear the appropriate PPE (safety goggles, face shield) to minimize splashes to the eyes, nose or mouth.

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5. As with all hazardous materials, please refer to the Safety Data Sheet (SDS) for safe handling guidelines. 6. Receiving, transporting, unpacking, and dispensing of flammables, reactive, or toxic chemicals must be carried out by trained personnel, and in such a manner to minimize the hazards. 7. Prior to using a chemical, verify that appropriate facilities and procedures are available for the safe disposal. 8. Ensure that safe storage facilities are available for unique physical or hazardous properties, such as temperature sensitive, water reactive, or explosibility. 9. Chemicals shall not be brought into a laboratory unless the lab’s design, construction and fire protection level is appropriate for the quantities of the chemical(s) involved. 10. Pressurized liquid dispensing containers shall comply with the following: - must be UL listed

- non-metallic containers larger than 4 L are prohibited

- relief devices shall discharge to a safe location

- piping/hose between the container and the use point shall be rated for the pressure,

compatible with the materials being transferred, and not subject to mechanical damage

- fittings must be secure and leak-free

- a readily accessible means to stop the flow of liquid from the container must be provided

- containers must be pressurized only with nitrogen or intert gas; air shall not be used

- a means to prevent backflow into the central gas supply system shall be provided

4.5 Storage and Segregation. Proper chemical storage within the laboratory is an essential part of any chemical safety program. Adhering to appropriate segregation of hazardous chemicals will lessen the risk of fire, accidental mixing during emergencies, and ultimately minimize employee exposure to hazardous chemicals. The following storage and chemical segregation requirements are mandatory for UTHSCSA facilities:

1. Be sure that all chemical components and mixtures are accurately labeled. 2. Containers used to store time-sensitive chemicals (i.e. organic peroxide formers) must be

labeled with the date received, and date opened. It is strongly recommended that all chemicals are labeled with the date received and date opened.

3. Flammable liquids should be stored in flammable liquid storage cabinets. Where feasible,

these cabinets should be vented.

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4. Acids, bases and flammable materials should be stored separately. In the event of an accident, the violent chemical reactions that can occur when certain compounds in these classes are mixed could exacerbate the situation.

5. If acids are stored in an acid storage cabinet, the cabinet is not required to be vented.

6. Acids and all corrosives shall not be stored under sinks. Storage space underneath sinks is

strictly for cleaning supplies. 7. Strong oxidizers, such a perchloric acid, should be stored away from organic materials to

reduce the risk of fire, and away from reducing agents to reduce the risk of violent reactions.

8. Cyanides and sulfides should be stored well separated from acidic compounds. The poisonous gases, hydrogen cyanide and hydrogen sulfide, are released when these compounds react with acids.

9. Toxics should be stored in a separate cabinet. Toxic chemicals that are also volatile should

be stored in a vented flammable cabinet. 10. Do not store hazardous liquid chemicals above eye level to avoid spilling liquids in the eyes.

11. Do not store glass containers on the floor.

12. Store acids and bases on the lowest shelves.

13. Do not indiscriminately store chemicals in alphabetical order. This can lead to incompatible

chemicals being stored next to each other. Dry chemicals that pose no hazards may be stored alphabetically on a shelf.

14. Periodically inspect storage areas. Note signs of leakage and corrosion. Make

arrangements to dispose of outdated materials or those no longer needed by the laboratory.

15. Use secondary containment (shallow plastic tubs) for storage of hazardous liquid chemicals to contain accidental leaks and spills.

16. Maximum allowable quantities of flammable liquids shall be reduced by 50% for Class B labs located above the third floor of a building (see Table 4-2).

17. Maximum allowable quantities of flammable liquids shall be reduced by 25% for Class C and Class D labs located fourth through sixth floors of a building (see Table 4-2).

18. Maximum allowable quantities of flammable liquids shall be reduced by 50% for Class C and Class D labs located above the sixth floor of a building (see Table 4-2).

19. Incompatible chemicals must be segregated to prevent accidental contact.

20. Time-sensitive chemicals must be managed properly, inspected frequently, and disposed through Environmental Health & Safety when determined to be unsafe. Contact Safety at 210-567-2955 for further guidance.

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4.6 Transporting Hazardous Chemicals within UTHSCSA. The following standard shall apply to all persons at any location considered part of the University of Texas Health Science Center at San Antonio. 1. An acid pail shall be any container impervious to attack by the material being transported but not made of glass, and be large enough to contain all of the material in case of breakage of the original container.

2. An acid pail shall be used to transport single glass containers of acids as well as other highly reactive material. The shipping carton may be used to transport quantities greater that one primary container.

3. Approved safety cans (for storage of liquids such as flammables, acids, or corrosives) shall be any container, of not more that 5 gallons capacity, having a spring-closing lid and spout cover and so designed that it will safety relieve internal pressure when subjected to fire exposure.

4. Flammable liquids shall be dispensed into and transported in approved safety cans only.

Non-flammable liquids shall be dispensed into glass containers. Glass containers with manufactures seal unbroken may be used to transport flammable liquids.

5. Transfer of material from one container to another which, at normal temperatures, gives off

toxic vapors, in quantities of one pint or less, shall be performed inside a chemical fume hood. Quantities greater than one pint shall be transferred only with leak-proof systems in well ventilated areas.

6. If a package of material is damaged so that there is probability of escape of the contents,

the Environmental Health & Safety shall be notified by the discoverer of the condition prior to acceptance or disposal of the material.

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5.0 Compressed Gases and Liquid Nitrogen Safety 5.1 Background. The use of compressed gases and liquid nitrogen presents many safety issues for laboratory staff and support personnel. This chapter will identify the hazards associated with compressed gases and liquid nitrogen, discuss the regulations issued by OSHA and NFPA regarding gas cylinder storage and handling, outline safety features for approved cylinders and cryogenic containers, and provide users with guidelines regarding safe use of these materials at UTHSCSA. 5.1.1 Compressed Gases. Compressed gases are used frequently by UTHSCSA employees for a variety of reasons. Laboratory personnel work with oxygen, nitrogen, and carbon dioxide during experimental procedures. Facilities Management personnel also require use of several compressed gases during the course of their job duties. Proper training on safe handling and use is critical for all employees and students who work with or around these materials.

a) Hazards: The hazards associated with compressed gases include physical hazards such as explosion or rupture of cylinders, and health hazards such as oxygen displacement or the toxic effects of certain gases.

b) Regulatory requirements: The Compressed Gas Association (CGA) has several

publications regarding safe handling of compressed gases. OSHA also has regulations regarding compressed gases, as outlined in 29 CFR 1910.101.

c) Inspection of cylinders:

1. All compressed gas cylinders should be visually inspected upon arrival to laboratories.

2. Verify the contents of the cylinders. All cylinders must be labeled at all times. 3. Labels all cylinders as to whether they are full, empty, or in use. 4. If a leak is detected, do not attempt to repair. Contact the supplier.

d) Storage of cylinders: 1. Secure cylinders at all times to prevent tipping, falling or rolling. Straps or

chains connected to a wall bracket or other fixed surface, or by using a cylinder stand. Straps and chains must be at 2/3 of the height of the cylinder.

2. Store cylinders in a cool, dry, well-ventilated, fire-resistant area. 3. Do not store cylinders in public corridors or stairwells. 4. Cylinders should be segregated by hazard:

i. Oxidizers must be stored separately from flammable gases. ii. Empty cylinders should be stored separately from filled cylinders.

5. Do not exceed the limitations on the number of cylinders allowed in a laboratory as set forth by NFPA: i. Three 10” x 50” flammable gas (acetylene, butane, hydrogen, vinyl chloride

ethylene oxide) or oxygen cylinders. ii. Three 4” x 15” cylinders of toxic gases (arsine, chlorine, fluorine, hydrogen cyanide, nitric oxide).

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e) Handling of cylinders: 1. Close valves for cylinders when not in use. Valves of empty cylinders must also

be closed. 2. Cylinders (filled, partially filled, or empty) shall be transported using an approved

cart or carrying device and must be securely fastened to the moving device so that accidental dislodgement does not occur.

3. Valves must be removed and protective caps secured in place prior to moving cylinders.

4. Valves should be closed prior to moving cylinders. 5. Empty cylinders should be removed from laboratories promptly. Contact

Receiving at 7-5998.

5.1.2 Liquid Nitrogen. This section discusses the potential hazards associated with cryogenic fluids, and outlines safety guidelines for handling, storage and transportation of liquid nitrogen. As a supplement to the guidelines described below, the Environmental Health & Safety Department has published a document, Laboratory Fact Sheet: Safety Guidelines for Working with Liquid Nitrogen (LN2). This fact sheet may be used as a training tool for laboratory workers who handle cryogenic materials. Contact the Safety Office to obtain a copy.

a) Hazards: The hazards associated with liquid nitrogen include the extremely low temperature (-320 oF), asphyxiation (oxygen displacement), and explosion or rupture of containers.

b) Regulatory requirements: The Compressed Gas Association (CGA) has several

publications regarding safe handling of liquid nitrogen. NFPA 55, Standard for the Storage, Use, and Handling of Compressed Gases and Cryogenic Fluids, outlines requirements for storage, use and handling of these materials.

c) Inspection and labeling of cryogenic containers and liquid nitrogen cylinders:

1. Visually inspect all containers upon arrival. 2. Inspect all valves, including vent valve, liquid valve, pressure relief valve, and rupture disk. 3. All cylinders or containers must be properly labeled at all times. Department of Transportation (DOT) marking must be affixed to all cylinders. 4. Labels all cylinders as to whether they are full, empty, or in use. 5. Portable cryogenic containers shall be marked in accordance with CGA C-7, Guide to the Preparation of Precautionary Labeling and Marking of Compressed Gas Containers. 6. All DOT-4L/TC-4LM liquid cylinders shall have product identification visible from all directions with minimum 51 mm (2 in.) high letters. 7. Visible hazard identification signs shall be provided in accordance with NFPA 704, Standard System for the Identification of the Hazards of Materials for Emergency Response, at entrances to buildings or areas in which cryogenic fluids are stored, handled, or used. 8. Container inlet and outlet connections, liquid-level limit controls, valves, and pressure gauges shall be identified by one of the following methods:

a) A permanent tag or label identifying their function.

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b) A schematic drawing that indicates their function and designates whether they are connected to the vapor or liquid space of the container.

c) When a schematic drawing is provided, it shall be attached to the container and maintained in a legible condition.

9. Hissing sounds from a liquid nitrogen cylinder is normal. Pressure build-up will be released through a pressure relief device. 10. If a leak or spill is detected, do not attempt to repair. Contact the supplier for assistance.

d) Storage of cryogenic containers: 1. Approved cryogenic containers shall be designed to hold low temperature,

liquefied gases and made of materials that can withstand the rapid changes and extreme differences in temperature encountered in working with liquefied gases. They shall be built to withstand normal operating pressures and shall be either open or protected by a vent or other pressure-relieving device that permits vapors to escape. Only vent tubes and stoppers supplied with these containers shall be used.

2. Store containers in a cool, dry, well-ventilated area. Oxygen monitors can be installed in areas where ventilation is not adequate. Contact Environmental Health & Safety for assistance.

3. Do not store containers or cylinders in public corridors or stairwells.

e) Handling and dispensing of liquid nitrogen or other cryogenic liquids: 1. Always use appropriate safety equipment, including cryogenic gloves, face shield and eye protection. 2. Containers (filled, partially filled, or empty) shall always be stored in the upright position. 3. Liquid nitrogen as well as other liquefied gases shall be dispensed into and transported in approved cryogenic containers only Use only approved containers or dewars. Do not use open pail-type containers. 4. Empty cylinders should be removed from laboratories promptly. Contact supplier for removal of empty liquid nitrogen cylinders. 5. Cryogenic containers transported on laboratory carts shall be secured to the cart to prevent accidental tip-over. Bungee cords are available on loan at General Stores.

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6.0 Evaluation of Special Chemical Hazards 6.1 Background. The purpose of this chapter is to provide information to Principal Investigators regarding special chemical hazards. Most laboratories work with hazardous chemicals and the activities are performed in accordance with institutional policies. The Environmental Health & Safety office provides support to the research staff for establishing safe work practices in the laboratory. To cover the specific needs of a particular laboratory, the principal investigator is responsible for preparing a safety protocol for the proposed research program involving highly hazardous chemicals prior to its beginning. These protocols should be reviewed with laboratory personnel on an annual basis. In addition, the Environmental Health & Safety Office, in conjunction with the Chemical Safety Committee will review all protocols involving work with carcinogenic, highly toxic, or acutely hazardous chemicals every three years to determine whether the principal investigator is required to submit any changes regarding research activities. Environmental Health & Safety personnel are available to provide information and assistance in preparing these safety protocols. 6.2 Special Hazards.

6.2.1 Carcinogens. OSHA publishes a list of known carcinogens that are strictly regulated. The regulations on each are specific and detailed. Their use involves a long list of requirements concerning recordkeeping, posting, monitoring, facilities, training, contamination control and medical surveillance. The following chemicals are on OSHA's list:

1,2-Dibromo-3-chloropropane 1,3-Butadiene 2-Acetylaminofluorene 3,3’-Dichlorobenzidine 4-Aminodiphenyl 4-Dimethylaminoazobenzene 4-Nitrobiphenyl Acrylonitrile Alpha-Naphthylamine

Asbestos

Benzidine

Beta-Naphthylamine

Beta-Propiolactone

Bis-Chloromethyl Ether

Cadmium Coke oven emissions Ethylene Oxide Ethyleneinine Formaldehyde Inorganic Arsenic Methyl Chloromethyl Ether Methylene Chloride Methylenedianiline N-Nitrosodimethylamine Vinyl Chloride

Although only a limited number of chemicals are carcinogens, they are found among all chemical classes and may present a number of hazards separate from their toxicity. Thus in any research laboratory where workers handle a wide variety of chemicals some are likely to be carcinogenic. The complexity of research at UT Health Science Center precludes establishing a single set of safety rules for working with carcinogens under all laboratory conditions. Institutional policies take cognizance of the following considerations:

(1) Carcinogens can be controlled using established laboratory procedures.

(2) Emphasis should be placed on engineering controls and good work practices.

(3) Carcinogens must be viewed individually and the biological, chemical and physical properties of each compound must be considered.

These guidelines below should be taken into consideration for all laboratories using carcinogens and other highly toxic chemicals:

1. Access to laboratories is limited to technical staff assigned to the research program and the necessary support staff.

2. Work should be performed in a suitable safety cabinet or other containment device depending on the nature of the experiment.

a. A glove box, Class II biological safety cabinet or chemical fume hood should be used for handling pure carcinogens, including the preparation of stock solutions for in vitro procedures or for work with concentrated carcinogen solutions.

b. Work with organic solvents and toxic or corrosive chemicals, including neutralization procedures should be done in a fume hood.

3. A hand washing facility must be available.

4. Vacuum service must be protected with an absorbent trap to prevent accidental contamination of the system.

5. Carcinogens should be stored in a clearly posted storage area preferably separated from other laboratory chemicals.

6. Stock bottles should be labeled with the full chemical name or a widely recognized substitute and should bear the warning “Potential Cancer Hazard” (NIH Guidelines), “Cancer Suspect Agent” (29CFR1910.1017, (l)) or “Chemical Carcinogen”.

7. Work surfaces should be protected with absorbent, plastic-backed bench paper.

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6.2.2 Asphyxiants. Chemical asphyxiants prevent or interfere with the uptake and transformation of oxygen. Examples include carbon monoxide which prevents oxygen transportation, and hydrogen cyanide which inhibits enzyme systems and interferes with the transportation of oxygen to the tissues. At sufficiently high concentrations, both chemicals can result in immediate collapse and death.

6.2.3 Narcotics. Narcotics affect the central nervous system causing symptoms that range from mild anesthesia reactions to loss of consciousness and death at high doses. Examples include acetone, methyl ethyl ketone, and chloroform.

6.2.4 Heavy metals and their compounds. Heavy metals are relatively harmless in the metallic state, but their fumes, dust, and soluble compounds are well-known toxins. Some are carcinogenic. Others are nephrotoxins, hepatotoxins, or neurotoxins. The most common heavy metals are arsenic, beryllium, cadmium, chromium, lead, mercury, nickel, and silver. Acute toxic effects from exposure to heavy metals result from inhalation and ingestion of dusts or inhalation of fumes. Metal fumes are generally more hazardous than dusts because the particles in fumes can enter the bloodstream easier. Bronchitis, chemical pneumonia, and pulmonary edema may result. Beryllium and cadmium are two of the most toxic metals when inhaled. Symptoms include nausea, vomiting, abdominal pain, and diarrhea. Chronic exposure to heavy metals may lead to long-term effects. For example, chronic exposure to lead may damage the nervous system, brain and kidneys. Exposure to mercury over a long period can permanently damage the liver, kidney, and brain. Chronic inhalation of cadmium can cause emphysema and kidney damage. Carcinogenic effects have been shown from exposure to chromium, nickel, arsenic, cadmium, and beryllium. Prenatal effects have been observed from exposure to methyl mercury. In addition, some lead compounds are embryotoxic. Some metals and their compounds can be absorbed through the skin. Mercury metal, and tetraethyl lead for example can enter the bloodstream through this route. Nickel, arsenic, chromium, and beryllium cannot penetrate the skin but they can damage the skin or cause allergic-type reactions.

6.2.5 Cyanides. The simple metallic cyanides are highly toxic by ingestion. Cyanides are readily absorbed through the skin, mucous membranes, and by inhalation. Alkali salts are toxic by ingestion. Even small amounts of sodium and potassium cyanide are highly toxic and death may occur within minutes from ingestion. Inhalation of toxic fumes from hydrogen cyanide gas may result in death in a few seconds. Symptoms of poisoning include dizziness, headaches, tightness in the chest, palpitation of the heart, and difficulty in breathing.

6.2.6 Nerve Agents. Nerve agents are the most toxic of the known chemical agents. They are hazards in their liquid and vapor states and can cause death within minutes after exposure. Nerve agents inhibit acetyl cholinesterase in tissue, and their effects are caused by the resulting excess acetylcholine. Nerve agents are considered major military threat agents. 6.3 Application for Approval to Work with Carcinogenic, Highly Toxic, or Acutely Hazardous Chemicals. The UTHSCSA Chemical Safety Committee established a peer-review approval process for carcinogenic and other highly toxic or acutely hazardous chemicals that can present a risk to human health. One of the charges of this Committee is to review procedures including the safe use, storage and disposal of hazardous chemicals, as a mechanism to ensure proper safeguards are being practiced in the laboratory, and thereby,

35

reducing occupational exposures to these chemicals. All Principal Investigators who wish to work with chemical carcinogens, highly toxic, or acutely hazardous chemicals at the Health Science Center usually do so as part of work on a grant or contract. All research protocols involving the use of chemical carcinogens, highly toxic, or acutely hazardous chemicals must complete the following:

1. Application for Approval to Work with Carcinogenic, Highly Toxic, or Acutely Hazardous Chemicals - for submittal to the Chemical Safety Committee through the Environmental Health & Safety Office. The Principal Investigator is responsible for completing the application in its entirety and providing the most accurate information. If the project or grant involves using chemical carcinogens in live animals, Appendix A: Application to Use Chemical Carcinogens with Live Animals must be completed. Classes of Chemicals Requiring Chemical Safety Approval

A. Carcinogens – any chemical which meets one of the following criteria:

a. Listed under Group 1 (carcinogenic to humans) or Group 2A (probably carcinogenic to humans) by the International Agency for Research on Cancer Monographs (IARC).

b. Listed as “Known to be Human Carcinogens” in the Annual Report on Carcinogens published by the National Toxicology Program (NTP).

c. Listed under the Occupational Safety & Health Administration (OSHA), 29 CFR 1910.1003.

Examples include, but not limited to Streptozotocin, DMBA, 12-0-Tetradecanoyl-phorbol- 13-acetate (TPA), and 3-methylcholanthracene. For the complete lists from IARC, NTP and OSHA, please refer to the Chemical Safety Handbook, Appendix C, C-1, and C-2 (http://research.uthscsa.edu/safety/Chemical/2007ChemicalSafetyHandbook.pdf). B. Antineoplastic Agents – chemotherapy and cytotoxic drugs used in cancer therapy.

Examples include Cyclophosphamide, Tamoxifen, Cisplatin, Daunorubicin, and Doxorubicin Hydrochloride.

C. Mutagens – chemical agents that alter DNA. Mutagens are also typically carcinogens. Examples include Urethane, Ethyl methanesulfonate, and Bromodeoxyuridine (BRDU).

D. Select Agent Toxins and other Toxins – Select Agent Toxins have the potential to pose a severe threat to animal health and safety, plant health and safety. Examples include tetrodotoxin and botulinum neurotoxin. Other toxins that would require CSC approval include cholera toxin (subunit A) and pertussis toxin.

E. Highly Toxic Chemicals – chemicals that have a high degree of acute toxicity. These

agents meet the following criteria: a. Median lethal dose (LD50) of 50 mg or less when administered orally to rats [LD50 <

50 mg/kg].

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b. Median lethal dose (LD50) of 200 mg or less when administered by continuous dermal contact for 24 hours (or less if death occurs within 24 hours) to the skin of rabbits [LD50 < 200 mg/kg].

c. Median lethal concentration (LC50) in air of 200 parts per million by volume when administered by continuous inhalation for one hour (or less if death occurs within one hour) to rats [LC50 < 200 ppm].

Examples include sodium arsenite, mercury, cadmium, and osmium tetroxide. F. Pesticides, Insecticides, Herbicides – defined as “any substance or mixture of substances

intended for preventing, destroying, repelling, or mitigating any pest” by the Federal Insecticide, Fungicide, and Rodenticide Act. Examples include Paraquat, Diquat, and Chlorpyrifos.

G. Pyrophoric Chemicals – substances that will ignite spontaneously upon contact with air. Examples include tertiary-butyl lithium, iron sulfide, and many reactive metals.

H. Nanoparticles – is defines as a small particles (1 - 100 nanometers) that behave as a whole unit in terms of its transport and properties. Some nanoparticles, such as Zinc Oxide, are thought to have cytotoxic properties.

I. Neurotoxins – chemical agents that can affect the transmission of signal between neurons. Examples include MPTP and snake venom.

For other highly hazardous chemicals that do not fall under these categories, please contact Chemical Safety Manager, at 567-2955 for assistance or to verify if a specific chemical requires CSC approval. Protocol Submission and Review Process: Chemical Protocols are reviewed on a continuous basis by the Committee. It is highly recommended that protocols are submitted well in advance of any funding or other agency deadlines. Principal Investigators must submit chemical protocols through the online E-Protocol system (https://uthscsa.keyusa.net/). After the application is received in the Environmental Health & Safety Office, the application will be reviewed for completeness prior to submitting to the Chemical Safety Committee. The Chemical Safety Committee will approve or disapprove each application usually within 30 days of receipt. An approval letter will be mailed to the Principal Investigator, and, if the project requires approval from the Institutional Animal Care and Use Committee (IACUC) approval, the Institutional Animal Care Program (IACP) office. If, for any reason, the application is not approved, the Principal Investigator will be notified as to the reason the application was not approved and will be given the opportunity to re-submit their application. 3. Administrative Approval for Use of Routine Carcinogenic or Highly Toxic Chemicals - Recently, the Chemical Safety Committee has revised the approval process for certain carcinogenic or highly toxic chemicals (see list below). The Committee acknowledges there are several carcinogens that are used routinely in labs, and when used in accordance to standard operating procedures (SOP), are relatively safe. So, instead of requiring Investigators to submit

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a chemical safety application through eProtocol, the use of these chemicals is being approved administratively by Environmental Health & Safety. A written SOP is provided to the Investigator/lab, to be reviewed by lab workers using the chemical, and after receiving the PI Acknowledgement form, an approval letter is drafted and sent to the PI. One benefit to this modified review process is a reduction of paperwork for the Principal Investigator.

Administrative approval is granted to Principal Investigators who work with the following routine carcinogens or highly toxic chemicals: Acrylamide Acetonitrile Boric Acid Chloroform Chloramphenicol Ethidium Bromide Formaldehyde (including10% formalin)

Methylene Chloride Paraformaldehyde Phenol Phenylmethylsulfonyl fluoride (PMSF) Phorbol 12-myristate 13-acetate (PMA) Sodium Azide Trypan Blue

Environmental Health & Safety has developed written standard operating procedures (SOP) which contains useful safety information normally found on the SDS for each chemical listed as well as emergency response and first aid treatment for these specific chemicals, and is meant to be used as a reference source for laboratory personnel.

6.4 Chemical Carcinogens Lists. The International Agency for Research on Cancer (IARC), the National Toxicology Program (NTP), and the Occupational Safety and Health Administration (OSHA) each have compiled lists of carcinogenic chemicals. These lists are not meant to be all-inclusive but rather, serve as a source for principal investigators in determining which chemicals may require committee approval for use and storage in the laboratory. For your convenience, these lists are included in Appendix B, C, and D. Please refer to the websites for updated lists.

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7.0 Monitoring of Hazardous Chemical Exposures 7.1 Hazard Determination. Before working with any chemical it is important to determine the hazards inherent in handling the material. Information about a chemical can be accessed by reading the container label or SDS. Other sources of information are available in determining safe exposure levels for healthy workers. One such resource is the American Conference of Governmental Hygienist (ACGIH) book of Threshold Limit Value (TLV) and Biological Exposure Indices (BEIs) for many common industrial chemicals. This document is used as a guide for the evaluation of workplace exposures to chemical substances and physical agents. TLVs are exposure levels to which a healthy worker can safely be exposed for 40 hours a week with no ill effects. Threshold limit values (TLVs) are used by OSHA in establishing Permissible Exposure Limits (PELs) to protect workers against the health effects of exposure to hazardous substances. PELs are regulatory limits on the amount or concentration of a substance in the air. They may also contain a skin designation. OSHA PELs are based on an 8-hour time weighted average (TWA) exposure. 7.2 Recommendation for Chemical Exposure Assessments. The following paragraph outlines several scenarios which would justify performing individual monitoring for chemical exposures:

1. Any procedures involving volatile or otherwise hazardous chemicals that are NOT performed in a chemical fume hood or ducted biological safety cabinet.

2. Other means of protection (respiratory) is not available to lab worker.

3. If any of the OSHA-regulated chemicals is being used. For a list of these chemicals, refer to 29 CFR 1910 Subpart Z – Toxic and Hazardous Substances.

4. Employee raises concerns.

7.3 Performing Chemical Exposure Assessments. The Environmental Health & Safety Office will perform personal monitoring on request for a variety of hazardous chemicals, including but not limited to chloroform, formaldehyde, xylene, isofluorane, and methylene chloride. An 8-hr time-weighted average (TWA) and a 15-minute short term exposure limit (STEL) will be determined during an average 8-hour work day while a hazardous chemical is in use. Badges will be analyzed by an accredited laboratory and results will be reported to the requesting employee and supervisor. Call Environmental Health & Safety, 567-2955, to make arrangements.

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8.0 Hazardous Chemical Inventory 8.1 Background. Under the Texas Hazard Communication Act, the University of Texas Health Science Center at San Antonio through the Chemical Safety Division of the Environmental Health & Safety Department will develop and maintain a list of hazardous chemicals normally present on all campuses. This workplace list, or chemical inventory, will be developed for each workplace area (i.e. laboratory, storage area, Central Energy Plant) and will be the responsibility of each Principal Investigator or Area Supervisor to review and update annually. 8.2 New Principal Investigators. New Principal Investigators are required to submit an inventory of hazardous chemicals to the Environmental Health & Safety Office. Instructions for submitting inventories are provided at the time of the New Research Laboratory Assessment. Chemical inventories should be submitted to the Environmental Health & Safety Office in a timely manner. 8.3 Annual Chemical Inventory Update. All new and existing Principal Investigators and area supervisors are required to update their hazardous chemical inventory annually. All hazardous chemical inventories will be updated by December 31 of each year. Memorandums are emailed to Principal Investigators in September, in order to provide ample time for review of inventories. Signed documentation of updates will be submitted to the Chemical Safety Manager in the Environmental Health & Safety Office, no later than December 31. 8.4 Common Use or Open Labs. An appropriate and accurate hazardous chemical inventory is required for all common use laboratories (e.g. environmental rooms) and open laboratories (e.g. CCRI, STRF). If a responsible faculty member is not apparent, then the appropriate Department Chair will be assigned responsibility. 8.5 Record Keeping and Forms. The Environmental Health & Safety Office will maintain copies of all hazardous chemical inventories in electronic and paper form. These chemical inventories will be maintained for 30 years by Environmental Health & Safety Office for review by employees and any regulatory agencies. Original documentation should be returned to the Environmental Health & Safety Office and a copy kept at each laboratory/area. A copy of the memo is provided at the end of this handbook (Appendix H).

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9.0 Annual Laboratory Safety Evaluations 9.1 Background. The purpose of the laboratory safety evaluation process is to anticipate, recognize, evaluate, and control potentially hazardous conditions in the laboratory. The Chemical Safety Division staff conducts periodic evaluations of each laboratory’s compliance with the most current version of the UTHSCSA Chemical Safety Handbook, Biological Safety Handbook and Bloodborne Pathogen Exposure Control Plan, Physical Safety Standards and Radiation Safety Handbook. Each UTHSCSA laboratory will be evaluated at least annually. This process will include a basic risk assessment and job hazard analysis for chemical, biological, radioactive material and physical hazards present in the laboratory, with the goal of reducing personal risk of exposure or injury, and loss of equipment and facilities. The laboratory safety evaluation process is designed to comply with environmental health and safety policies and regulations including, but not limited to: (1) The four UTHSCSA Safety Handbooks (2) The Texas Hazard Communication Act, 25 TAC §295 (3) Texas Department of Health, Bloodborne Pathogen Control, 25 TAC §96

(4) TNRCC / TCEQ regulations on Spill Prevention and Control (30TAC §330) (5) Regulations adopted by the State Fire Marshal including NFPA 45 – Standard on Fire Protection for Laboratories Using Chemicals (6) Biological containment recommendations as promulgated in HHS Publication No. (CDC) 93- 8395, Biosafety in Microbiological and Biomedical Laboratories, 5th Edition. 9.2 Laboratory Evaluation Record Report. The primary tool for accomplishing the evaluation is the Laboratory Safety Evaluation Record, which is completed by Safety Specialists in the Chemical Safety Division of the EH&S Office. A copy of this record can be found in Appendix F of this handbook. This evaluation record consists of 66 primary safety considerations divided into the following categories:

1. Hazard Assessment Section: describes the location, PI, and contact person for the lab. Items 1-7 of this section include room type, basic laboratory hazards present, personnel, and safety training.

2. General Postings Section (items 1-9): includes posted hazards, emergency contact information, and required notices to be posted in the lab.

3. General Safety Section (items 10-17): required UTHSCSA safety policies (Safety Handbooks and Procedures), training records, general housekeeping, food & drink policy.

4. General Indoor Air Quality (IAQ) Section (items 18-20): covers general indoor quality issues including presence of unusual odors, correct room air pressure, and visible particulates from air vents.

5. Fire Safety Section (items 21-29): covers questions pertaining to the UTHSCSA Physical Safety Standards Manual and NFPA 101 Life Safety Code: fire egress, access to pull stations and fire equipment, availability of fire extinguishers, smoke detectors, self-closing doors to labs, access to electrical panel and emergency gas shut-off valve, proper labeling and function of natural gas lines, heat sources away from combustibles, and clearance to sprinkler heads, lights and air vent ducts.

6. Physical Safety Section (items 30-36): applies to topics covered under the OSHA General Industry Standards: slips, trips, cut hazards, mechanical hazards, electrical hazards, and tip over hazards of compressed gas cylinders.

7. Biological Safety Section (items 37-48): applies to standard microbiological practices and general biological safety standards as outlined in the UTHSCSA Biological Safety

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Handbook & Exposure Control Plan, TDSHS / OSHA bloodborne pathogens regulations, and regulated medical waste.

8. Chemical Safety Section (items 49-66): Questions 49-62 address issues related to chemical safety as outlined in the UTHSCSA Chemical Safety Handbook, Texas Hazard Communication Act, and NFPA 45. Questions 63-64 address TNRCC / TCEQ hazardous chemical waste compliance. Items 65-66 address previous deficiencies and any additional items not covered in the survey.

9.3 The Actual Audit and Follow-up Procedures. The safety evaluation will be scheduled by Safety Specialists in the Chemical Safety Division in coordination with the principal investigator and/or lab manager. EH&S personnel will meet with the Principal Investigator and/or the lab manager for a brief assessment of hazards present in the lab and to discuss the scope of research or procedures performed in each lab. The Principal Investigator and lab personnel have the opportunity to ask any safety-related questions at any time during the evaluation. The Safety Specialist will spend approximately 30 minutes to one hour conducting the evaluation and will identify any discrepancies or deficiencies related to the specific items listed in the laboratory evaluation record report. Some deficiencies may be corrected at the time of the evaluation with the help of EH&S personnel. Other deficiencies, however, may require some action by lab personnel or PI. After the evaluation has been completed, the Safety Specialist will conduct a close-out meeting to review any deficiencies found during the survey. An email will be sent to the PI and lab manager which summarizes the safety concerns found during the evaluation. Details regarding any safety concerns, as well as copies of the reports, can be viewed on the EHS Assistant online database (http://ehsaweb.uthscsa.edu). The PI has 30 days to correct any deficiencies and submit corrective actions online. Any deficiencies not corrected within the timeframe (normally 30 days) will be followed up by an e-mail or second written notification to the respective PI or lab manager by the evaluating Safety Specialist. Depending on the severity of the deficiencies noted, an Escalated Deficiency Notification letter may be sent to the responsible PI from the Environmental Health & Safety Director, or the Chemical Safety Committee Chair. Extremely serious situations where the PI fails to respond may necessitate communication to the appropriate Dean, Executive Vice President or UTHSCSA President. 9.4 Some Common Laboratory Deficiencies and Corrective Measures. For every deficiency that is noted in the evaluation report, a suggested corrective action will also be noted on the report. Deficiencies related to required postings, availability of safety manuals, and the UTHSCSA Emergency Evacuation & Response Plan will be corrected at the time of the survey by Environmental Health & Safety. Required safety training will be the responsibility of the employee to correct. Items found that involve adjustments of air flow, fume hood checks, and other building maintenance issues will be corrected by Facilities Management personnel at the request of Environmental Health & Safety. Deficiencies related to safe storage of hazardous chemicals and/or waste will be corrected by laboratory personnel.

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Table 9-1 below is a compilation of the ten most frequent laboratory safety deficiencies and recommended corrective actions:

Observed Deficiency Corrective Action 1. Appropriate safety training records were not available in EH&S office.

All lab personnel are required to attend mandatory safety training. Documentation of attendance is maintained by EHS office.

2. Secondary containers were not labeled properly, or unlabeled secondary containers.

Affix a proper label to all secondary containers. The Safety Office can provide labels at no cost.

3. Additional safety concerns were noted during the evaluation.

Specific recommendations are provided depending on the concern. Contact the Safety Office for additional information.

4. Chemicals were not stored or segregated properly.

Segregate chemicals according to hazard class. Utilize secondary containment for storage of hazardous liquid chemical containers.

5. Chemical Safety Committee approval not on file or requires updating.

PI is required to submit application for use of certain carcinogenic or highly toxic chemicals.

6. Regulated medical waste/sharps were not properly stored or handled. Containers were over-filled.

Fill bio boxes or sharps containers to the fill point (~3/4 full). Keep weight under 30 pounds. Dispose of gloves and other lab-associated waste in bio boxes and needles and other sharps material into sharps container.

7. General housekeeping was not orderly. Maintain clean work spaces. Discard old or

soiled diaper pads, and remove or discard unused equipment.

8. Chemical waste was not properly stored or liquid waste containers were not capped/closed.

Chemical waste containers must be stored in secondary containment. Waste containers must be capped at all times unless in immediate use.

9. Institutional Biosafety Committee (IBC) approval was not on file or not current.

PI is required to submit application for use of certain biological agents, including recombinant DNA.

10. Adequate clearance (18”) was not maintained to ceiling, sprinkler heads, air diffusers, or light fixtures.

Remove items located within 18 inches to ceilings, sprinkler heads, air diffusers and light fixtures.

9.5 Self-Assessment of Laboratories. Routine laboratory safety surveys conducted by Environmental Health & Safety are performed at least every two years. Therefore, to help maintain a safe laboratory environment between independent surveys, self-assessments may be performed by

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lab personnel or the Principal Investigator. The Laboratory Safety Evaluation Record (Appendix I) can be a useful tool. During these assessments, procedures can be reviewed along with employee training requirements, chemical storage requirements, and waste management procedures. This practice will help to reduce or eliminate deficiencies that may be identified during the EH&S surveys. 9.5.1 Job Safety Hazard Analysis. Upon request, Environmental Health & Safety will conduct a Job Safety Hazard Analysis. During this process, specific job functions/procedures will be observed and reviewed by a staff member from Environmental Health & Safety. Equipment, materials and supplies, and procedural steps will be observed and any related hazards will be noted. Based on the findings, recommendations for procedural modifications will be made in a effort to minimize employee exposure to hazards. Please call Environmental Health & Safety, 567-2955, to make arrangements for this service. 9.6 New PI Laboratory Risk Assessment. New Principal Investigators will be contacted by Environmental Health & Safety for a New PI Laboratory Risk Assessment. During this meeting, information will be obtained regarding the nature of research and information will be given to the new PI for submittal of protocols involving use of hazardous chemicals, biological agents, and radioactive material. Additionally, information will be provided regarding UTHSCSA policies on training, waste management, chemical inventory and medical surveillance. Assistance will be given in setting up new laboratories. All postings and hazard stickers will be provided by Environmental Health & Safety.

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10.0 Laboratory Hygiene & Personal Protective Equipment 10.1 Personal Hygiene, Habits, and Practices. Personal hygiene in the laboratory is directed mainly toward the prevention of occupationally-acquired disease or physical injury. However, it can raise the quality of laboratory work by reducing possibilities for contamination of experimental material. Habitual adherence to good practices provides a margin of safety in situations where the hazard may be unrecognized. The history of occupational injury is replete with examples of hazards unrecognized until too late. The following guidelines are standard operating procedures. 1. Food, candy, gum, beverages, and tobacco for human consumption shall not be stored or consumed inside the laboratory or animal rooms. 2. Drinking fountains outside the laboratory should be the sole source of drinking water. 3. Refrigerators in laboratories are for experimental materials only. Food for human consumption shall be stored only in refrigerators specifically designated for that purpose. 4. Do not use laboratory equipment for food preparation. Do not use empty food containers for laboratory materials or samples. 5. Smoking is not permitted in laboratories or animal rooms, or anywhere on UTHSCSA campuses. 6. Shaving or brushing teeth is not permitted in laboratories. Toothbrushes, razors, toiletry supplies, and cosmetics should only be used in designated areas outside the laboratory after thoroughly washing the hands and face or showering. 7. Facial hairs are discouraged in areas where there is the potential for airborne hazardous materials. Facial hair retains particulates more persistently than clean-shaven skin. A clean-shaven face is essential to the adequate fit of a face mask or respirator when the work requires respiratory protection. 8. Keep hands away from the mouth, nose, eyes, face and hair when working in the laboratory. 9. Books and journals should be used only in clean areas if possible. 10. Personal handkerchiefs should not be used in the laboratory. Disposable tissues should be available in laboratories and change rooms. 11. Shorts and sandals are prohibited from being worn in all UTHSCSA laboratories. Appropriate clothing is required. Choose sturdy shoes that cover the whole foot and protect from spills and broken glass. Wear a knee-length, long sleeve lab coat (buttoned) to protect skin & clothing. Lab coat sleeves must cover arms & shirts. Wear long pants (or equivalent) to protect from spills & splash. Avoid loose or synthetic clothing. Remove loose jewelry. Secure hair and clothing away from flames, equipment, and chemical contamination.

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10.2 Laboratory Housekeeping. All UTHSCSA laboratories must be managed and well- maintained as a safe work environment. General housekeeping is important when it comes to safety. The UTHSCSA Housekeeping staff provides limited services such as emptying the regular trash receptacles. Therefore, it is the responsibility of the Principal Investigator and lab workers to practice good housekeeping techniques on a regular basis. The following guidelines will keep laboratories organized and provide a safe workplace:

1. Keep laboratory benches clear of un-needed clutter.

2. Keep all hazardous chemical containers clearly labeled and stored in approved areas such as underneath chemical fume hoods, or in flammable storage cabinets.

3. Keep large boxes of supplies off of floors and bench tops.

4. Perform routine clean-outs to remove all unwanted chemicals, equipment, or supplies. Broken, unwanted or laboratory equipment requiring service or repair must be cleared by Environmental Health & Safety. Requests for equipment clearances can be submitted from the EHS website (http://research.uthscsa.edu/safety/equip-clearance.shtml). 5. Unwanted, expired, or spent chemicals must be picked up by Environmental Health & Safety. Chemical pick-up procedures are outlined in Chapter 14.

6. Ensure all waste materials (chemical, biological, and radiological) are disposed of according to UTHSCSA policies.

7. Promptly clean up spills. Hazardous material spills must be reported to Environmental Health & Safety.

8. Broken laboratory glassware must be placed in a sturdy cardboard box (do not use a regulated medical waste box). Once full, the box should be closed securely and marked “Broken Glassware”. Housekeeping staff will collect these boxes, which are examined by Environmental Health & Safety staff prior to placing in the compactor, located outside General Stores. 10.3 Availability of Personal Protective Equipment. A variety of personal protective equipment (PPE) is available which, when correctly used, helps protect laboratory workers from hazards in the work environment. PPE must be provided by the responsible supervisor/Principal Investigator. At a minimum, all employees who work with hazardous chemicals in any laboratory at UTHSCSA are required to wear the following:

a) Closed toe shoes (no sandals) b) Long pants or skirts (no shorts) c) Lab coat, apron or gown d) Safety glasses or chemical goggles e) Gloves (latex, nitrile, vinyl, or other appropriate gloves)

Additional PPE may be necessary, depending on the procedures and exposure risks involved. Always refer to the SDS for recommended PPE for specific chemicals. It is the responsibility of each worker to wear the appropriate PPE when necessary and to talk with your supervisor if

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questions arise regarding additional PPE that may be needed. Environmental Health & Safety will perform exposure assessments as a means to determine additional PPE requirements. 10.4 Training for Personal Protective Equipment (PPE). Training on use and maintenance of PPE is the responsibility of the PI/supervisor. Use of most PPE requires very minimal instruction and maintenance. However, specialized equipment such as respirators requires additional training including a medical evaluation, fit test and instruction on how to don and remove a respirator, and routine cleaning as well as choosing the right type of filter. A fit test will identify the proper size for the worker, and ensure that the respirator will provide maximum protection from exposure to hazardous materials. Do not wear any respirator, such as an N95, half-face, or full- face respirator, without completing a fit test. Under the UTHSCSA Respiratory Protection Program, Environmental Health & Safety will assist all employees who are required to wear a respirator as part of his or her job duties.

10.5 UTHSCSA Policy on Wearing Gloves. It is mandatory gloves be worn during situations where exposure to hazardous materials (chemical, biological, radiological) is imminent. Protection of the employees from hazards is top priority. Additionally, one should regularly change gloves when contamination has occurred. One should always take steps to avoid spread of contamination. Therefore, gloves must always be removed upon exit from any laboratory or other areas where hazardous materials are used, processed, or stored. Please dispose of all used gloves in the approved medical waste containers. It is extremely important that all public areas are kept clean at all times. It is everyone’s responsibility to eliminate unnecessary contamination throughout UTHSCSA property. The UTHSCSA Chemical Safety Committee does not tolerate glove use during the following activities:

• Upon exiting laboratories

• Opening doors (labs,

restrooms, stairwells, lecture halls, etc.)

• Walking through public

hallways

• Using elevators

• Using telephones

• Using water fountains

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When transporting hazardous materials through the public corridors: 1. Use secondary containment (clean plastic tub or cart) to safely move these materials from one area to another. Secondary containment not only provides protection for the worker, but also provides a means of containment in case of accidental spills or breakage. 2. Lab carts should have a lip to protect containers from tipping over during transport. 3. Do not carry samples or hazardous materials directly in your hands. Non-compliance with this policy should be reported to the Environmental Health & Safety Department. All violations will be reviewed by Environmental Health & Safety. The first offense will result in a visit from Environmental Health & Safety for remedial training. A letter from the director of Environmental Health & Safety documenting such offense will be sent to the employee and their supervisor. All repeat violators will be required to report to the Chemical Safety Committee.

Table 10-1 below shows information about different types of personal protective equipment and the hazards for which they may be used.

Types of PPE Applications

Safety glasses Chemical goggles

Face shield

Protection of eyes when working with hazardous chemicals which are irritating, corrosive, or toxic by contact or absorption. Provides impact protection when needed. Splash protection from acids or other corrosive materials when pouring or transferring. Face shields provide protection of entire face.

Closed toe shoes Shoe covers

Protect feet from exposure to hazardous chemicals, especially during accidental spills or breakage.

Lab Coat Chemical resistant Apron

Lab coats offer full body protection from contamination and chemical splashes. Chemically-resistant aprons provided heavy duty protection when working with hazardous chemicals.

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Types of PPE Applications

N95 respirator Full face respirator

Half face respirator * Require fit testing and medical evaluation prior to use. Contact Environmental Health & Safety, 7-2955

N95 respirators provide protection against organic vapors, dusts, microorganisms, and non-oil containing mists. Full face respirators provide protection against organic vapors. Different types of cartridges available for various chemical applications. Also provides added face protection. Half face respirators provide protection from organic vapors. Various cartridge types for different chemical applications. Acceptable when face protection is not needed.

Earplugs Earmuffs

Noise levels above recommended action level of 85 dB for prolonged periods of time. Noise reduction rates range from 20 to 33 dB.

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11.0 Laboratory Ventilation and Chemical Fume Hoods 11.1 Background. Laboratory ventilation systems are an important component to any Chemical Hygiene Plan. They ensure a source of uncontaminated air for breathing, as well as exhaust ventilation for elimination of potentially contaminated air. Finally, they ensure that fire hazards and risks are minimized. This chapter outlines several key components to maintaining adequate laboratory ventilation. 11.2 General Laboratory Ventilation. General ventilation requirements for labs must consist of the following: - Source of clean air for breathing and for input to local ventilation devices - Should not be the only means for protecting workers against toxic substances released into the laboratory - Ensure that laboratory air is continually replaced - Provide directional air flow from non-laboratory areas and exhausted out to the exterior of the building At UTHSCSA, most laboratories are designed with negative directional air flow with respect to the corridor. One exception is tissue culture rooms, which are required to have either neutral or slightly positive air flow, as a way to reduce contaminated air into these clean environments. 11.3 Chemical Fume Hoods. Chemical fume hoods are an integral part of the ventilation system for laboratories. They serve to exhaust hazardous substances that have been released in the laboratory, thereby providing a safe working environment for workers handling and dispensing volatile, highly toxic, or otherwise acutely hazardous materials. 11.3.1 Types of Chemical Fume Hoods Constant Air Volume Hood (CAV) – face velocity is inversely proportional to the sash height. The volume of airflow within the hood is maintained constantly. All air enters through the sash opening. Most chemical fume hoods at UTHSCSA are CAV hoods.

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Bypass Hood - This type of hood is similar to CAV. The only difference is that it has an air bypass that provides an additional source of ambient air when the sash is closed.

Auxiliary Air Hood – This type of hood is similar to a bypass hood. The only difference is that the air is supplied from a dedicated duct that brings in air from outside of the building.

Variable Air Volume Hood (VAV) – maintain a constant face velocity regardless of the height of the sash. The exhaust volume is adjusted when the sash is moved so that the average face velocity is maintained within set parameters.

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Snorkel – provide local exhaust, but only provide up to 45 linear feet per minute (fpm).

Canopy Hoods – require a large amount of air movement to effectively exhaust contaminants

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11.3.2 Hood Components

11.4 Fume Hood Schematics. Chemical fume hoods are designed such that air flows through the hood at an average velocity of 100 linear feet per minute. The diagram below illustrates with arrow the direction of airflow.

11.5 Fume Hood Work Practices. Several important factors should be considered to ensure proper function, as well as minimization of worker exposures while working with hazardous materials. Below is a summary of these considerations: 1. Keep the inside of the hood clean and uncluttered. Overcrowding the work space may obstruct air from being adequately exhausted. Limit chemical storage inside work area of fume hoods. Use storage area below the hood to store stock bottles.

Sash – Sliding glass door in front of hood. Airfoil – Located along the bottom “lip” of hood; streamlines airflow into the hood Work Surface – work area of hood Baffles – The adjustable slates along the back of the hood body Exhaust plenum – channels air from hood to the exterior. Helps to distribute airflow evenly across the face of the hood

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2. Keep the sash at or below the required height while working. A good rule of thumb is to lower the sash to chest level. Never work with the sash fully open. Note in the illustration below the difference in the distance between the contaminants and the worker’s breathing zone. The lowered sash increases the distance between your breathing zone and the area where contaminants may escape.

3. Always work about 6 inches from the sash line. This helps to reduce exposures as air currents are more turbulent at the face of the hood. Note in the illustration below the different locations of the beaker in the fume hood.

4. Verify directional airflow by taping a piece of KimWipe© to the sash. Newer chemical fume hoods are equipped with an airflow monitor.

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5. Wear appropriate PPE (lab coat, gloves, safety glasses, etc.). Keep your face outside the planes of the hood sash.

YES NO 11.6 Annual Testing. Chemical fume hoods at UTHSCSA are tested at least annually according to the ANSI/ASHRAE 110-1995 Standard, Method of Testing Performance of Laboratory Fume Hoods. Testing is performed by Facilities Management personnel, and the results are kept on file in the Environmental Health & Safety Office. Adequate face velocity should be between 80-120 linear feet per minute (lfpm). This information is written on the yellow certification sticker affixed to the hood.

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12.0 Shipping of Hazardous Chemicals 12.1 Background. The shipment of hazardous materials must follow rigorous regulatory requirements from the Department of Transportation (DOT) and the International Air Transport Association (IATA). If you are involved in the shipment of any hazardous materials or substances, you must receive training on the regulations and requirements prior to shipping each class or division of materials. For instance, if you have been trained in the past two years on shipping dry ice (Class 9) that does not permit you to ship an explosive (Class 1). Hazardous materials must be shipped utilizing a hazardous material shipper. FedEx currently ships hazardous materials. UPS and DHL require an agreement for hazardous material shipments. These types of shipments must be generated with eShip Global, an online system for creating shipping manifests. Only trained individuals are permitted to access eShip Global. 12.2 Hazard Classifications and Divisions. Below is a summary of the hazard classifications and division per DOT:

Hazard Class Division

1: Explosives 1.1 mass explosion hazard 1.2 projectile hazard 1.3 minor blast/projectile/fire 1.4 minor blast 1.5 insensitive explosives 1.6 very insensitive explosives

2: Compressed Gases 2.1 flammable gases 2.2 non-flammable compressed 2.3 poisonous

3: Flammable Liquids Flammable (flash point below 141°) Combustible (flash point 141°-200°

4: Flammable Solids 4.1 flammable solids 4.2 spontaneously combustible 4.3 dangerous when wet

5: Oxidizers and Organic Peroxides

5.1 Oxidizer 5.2 Organic Peroxide

6: Toxic Materials 6.1 Material that is poisonous 6.2 Infectious Agents

7: Radioactive Material Radioactive I Radioactive II Radioactive III

8: Corrosive Material Destruction of the human skin Corrode steel at a rate of 0.25 inches per year

9: Miscellaneous A material that presents a hazard during shipment but does not meet the definition of the other classes

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12.3 Training Requirements. Shippers of hazardous materials MUST BE BOTH TRAINED AND CERTIFIED for each hazard class or division prior to shipment. Hazardous Material Regulations (HMR) apply to anyone who handles, offers for transport, transports hazardous materials, causes hazardous materials to be transported or performs any hazmat functions related to transport in commerce (49 CFR 171.1). The US variations published in the ICAO technical instructions are also followed in 49 CFR 171.22. Any violation of hazardous material regulations may be subject to a civil penalty of up to $50,000 for each violation and in certain cases a criminal penalty of up to $500,000 and/or imprisonment for up to five years. Penalties double when the violation results in a serious injury or death. Training for shipments of hazardous chemicals must comply with Department of Transportation (DOT) and International Air Transport Association (IATA) regulations, must be completed every two years, and must be documented. For assistance in training availability, please contact Environmental Health & Safety, 210-567-2955. 12.4 Record Keeping. Maintain a valid record of completion of shipping training and provide a copy to the Environmental Health & Safety Office, 1.343T DTL. Record keeping requirements are further discussed in Chapter 17.

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13.0 Training 13.1 Hazard Communication. Under the Texas Hazard Communication Act, all UTHSCSA faculty, staff, and students are required to complete HazCom training, also called Safety Awareness training, offered through New Employee/New Faculty Orientation. This general training session covers topics related to the various categories of hazardous materials present here at UTHSCSA. In addition to awareness of the various hazardous chemicals used at UTHSCSA, employees and students are notified of the radiological, biological and physical hazards that are encountered here. Under the University’s Hazard Communication plan, faculty, staff and students have certain responsibilities with respect to hazard communication. Principal Investigators are responsible for determining who among their staff fall under the provisions of the Act. They are to have their new staff attend a mandatory Safety Awareness training given by Environmental Health & Safety at New Employee Orientation. New faculty is also required to attend this training. Principal Investigators are then responsible for training staff members concerning the hazards of the specific chemicals in their laboratories. To ensure that knowledge is kept current the Act requires that training be performed as needed and documentation be maintained. Principal Investigators are also responsible for ensuring compliance with the labeling requirements of the Act. Staff members are responsible for only using chemicals for which they are adequately trained. They are expected to identify hazardous chemicals in the workplace by using the chemical lists provided in the handbook or by determining if the chemical falls under any of the hazard categories as discussed in this manual. Staff are expected to consult references, including Safety Data Sheets (SDS) to determine hazard characteristics and handling procedures. The staff are expected to follow supervisor’s instructions regarding the use of hazardous chemicals and to observe the guideline stated in this document. 13.2 Laboratory Safety & Hazardous Waste Generator’s Training. All new laboratory staff and faculty are required to attend the Laboratory Safety & Hazardous Waste Generator’s Training Course. The following topics are discussed: a) Interpretation of SDS b) Safe handling, storage, and disposal procedures c) First aid treatment d) General lab safety practices e) Chemical classifications and their associated hazards f) Personal protective equipment and resources available for safely working with hazardous chemicals g) Safety equipment (including emergency eyewashes and safety showers) h) Emergency response procedures a) Institutional policies and procedures for proper management of chemical, radiological and biological waste b) Local, state, and federal regulations regarding hazardous waste management c) Recycling programs established at UTHSCSA. This training is a one-time training requirement, although refresher training may be deemed necessary if safety protocols established in this manual are not followed which demonstrates a lack of fundamental lab safety knowledge.

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13.3 Site Specific Training. Supplemental site specific training, called Site-Specific Chemical Hazard training, should be given by the Principal Investigator or supervisor for all employees who work with hazardous chemicals. To ensure that knowledge is kept current, the Texas Hazard Communication Act requires that training is performed as needed and documentation is maintained for 5 years. The Environmental Health & Safety Office provides a training form entitled Principal Investigator’s / Supervisor’s Hazard Communication Act Specific Training Form which can be accessed through the EH&S website, http://research.uthscsa.edu/safety/ and can also be found in Appendix G at the end of this handbook. This training session should cover interpretation of SDS, safe handling, storage, and disposal procedures for specific chemicals used in their immediate area. Additionally, emergency response procedures and first aid treatment should be discussed. 13.4 Incidental Training. Incidental training may be required, depending on specific requests by Principal Investigators, or as a result of deficient laboratory safety protocols outlined in the Chemical Safety Manual. 13.5 Record Keeping. All chemical training records will be maintained in the Environmental Health & Safety Office, 1.343T, DTL. Documentation for any safety training should also be kept in the laboratories or departmental office. Record keeping requirements are further discussed in Chapter 17.

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14.0 Hazardous Chemical Waste Management 14.1 Responsibilities. The hazardous waste management program at the UTHSCSA is designed to provide safe, effective and economical disposal of chemical waste generated by the institution in compliance with the Resource Conservation and Recovery Act (RCRA), Clean Water Act (CWA) and Clean Air Act (CAA). The following sections cover the basis for the program, the legal definitions of hazardous chemical waste and the system developed to manage hazardous waste on campus. The Environmental Health & Safety Office staff is responsible for overseeing the day to day operations of the hazardous waste program. This includes supervising the Environmental Health & Safety staff, procedures and facilities; conducting inspections of campus laboratories and work areas; and evaluating the methods used for hazardous waste management. Principal Investigators are responsible for all activities in their laboratories. They train their staff and ensure that hazardous materials are identified, collected and stored properly and that appropriate records are kept. Individual laboratory workers are responsible for the day-to-day activities in the program. They follow the directions of the principal investigator, collect waste and keep records. Facilities Management workers are responsible for proper collection and storage of the wastes created in their operations. They are also responsible for performing the necessary maintenance on systems vital to the safe operation of the waste handling facilities. The campus police provide security and ensure that access to the hazardous waste facilities is controlled. They also serve a central role in emergency situations. 14.2 Legislative Background and Requirements. The Resource Conservation and Recovery Act of 1976 (RCRA) and the Hazardous and Solid Waste Amendments of 1984 (HSWA) were passed by Congress to provide the legislative basis for resource and hazardous waste management in the United States. The Environmental Protection Agency (EPA) is the federal agency responsible for the administration and enforcement of RCRA. In Texas, EPA has delegated authority for administrating and enforcing RCRA regulations to the Texas Commission on Environmental Quality (TCEQ). This delegation does not exclude the EPA from interceding with its own enforcement should it find that TCEQ is not properly carrying out the implementation of the regulations. RCRA and the TCEQ regulations provide for the cradle-to-grave management of hazardous waste and regulate the operation of waste disposal operations. The regulations are designed to provide for the protection of public health and the environment. They set forth very stringent requirements for generators, transporters and processors of hazardous waste. The UTHSCSA is subject to regular inspections by TCEQ, San Antonio Water System (SAWS), and EPA to ensure that the program is operating under the conditions of the permit. As part of these inspections, waste generating areas on campus including laboratories and shops are subject to inspection by these agencies. 14.3 Definitions of Hazardous Waste. For regulatory purposes a hazardous chemical is one that poses a danger to human health or the environment because of physical or toxicological properties. The RCRA regulations define hazardous waste in terms of four specific hazard characteristics, ignitability, corrosivity, reactivity and toxicity. In addition, the EPA has compiled two lists, one of acutely hazardous material (the “P” list) and one of toxic waste (the “U” list). To determine if a waste is hazardous, it should be first checked against the EPA lists. P and U listed waste are indicated in lists provided at the end of this chapter. If the chemical is not on the P or U lists, it must then be examined to determine if it meets any of the criteria indicated below:

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1. Ignitability a. Liquids with a flash point of less that 60oC (140oF). b. Solids that may ignite readily through friction, absorption of moisture or spontaneous

chemical change. c. Ignitable compressed gases. d. Oxidizers. e.

2. Corrosivity – any aqueous solution with a pH less than or equal to 2 or greater than or equal to 12.5 or any material that will corrode steel (1/4” per year).

3. Reactivity – this includes material with any one of the following characteristics:

a. Normally unstable and undergoes violent change without detonating. b. Reacts violently with water, or forms a potentially explosive mixture with water. c. Forms toxic gases, vapors or fumes when mixed with water. d. May explode upon heating, or by physical shock, or from decomposition.

4. Toxic Characteristic Leaching Procedure (TCLP) – any waste which contains any of the elements or compounds in concentrations equal to or greater than the maximum concentrations given in the list for toxic characteristic constituents and regulatory levels provided at the end of this chapter.

Compliance must also be maintained with San Antonio Water System (SAWS). The UTHSCSA Industrial Wastewater Permit specifies what cannot be put down the sanitary sewer. It prohibits the introduction of wastes with a pH less than 5.5 or greater than 10.5 or wastewater having any other corrosive property capable of causing damage or hazard to structures, equipment, and/or personnel of the water treatment facility. It is most important that toxic or reactive materials not be put into the sanitary sewer system. This includes materials such as sodium azide, chromic acid and perchloric acid. Many laboratory chemicals used at UTHSCSA are known to be toxic but are not on the EPA-regulated hazardous waste lists nor do they meet the other guidelines stipulated. Chemicals that are known or suspected of being toxic, carcinogenic, mutagenic or teratogenic should be handled as hazardous waste. In these cases, the principal investigator must assess the risk associated with the material and determine an acceptable disposal method. Information on toxicity can be found in the Registry of Toxic Effects of Chemical Substances (RTECS), published by the National Institute for Occupational Safety and Health (NIOSH).

14.3.1 Pharmaceutical Waste. Some pharmaceutical items can be characterized as non-RCRA hazardous materials. RCRA listed or characteristic hazardous pharmaceutical waste cannot be disposed of through sewers, landfills, or through regulated medical waste. It must be treated as hazardous chemicals and shipped to a RCRA facility. 14.3.2 Controlled Substances. Controlled substances are regulated through the Drug Enforcement Agency (DEA) and users must register with that agency. Controlled substances cannot be treated as hazardous chemicals with respect to disposal, and therefore must not be discarded as hazardous waste. Disposal of all expired or unused controlled substances must be done through University Police. A DEA Form 41, Registrant’s Inventory of Drugs Surrendered must be filled out to surrender Schedule I, II, III, IV or V controlled substances for disposal. UTPD retains all documentation for disposal of controlled substances and scheduling information.

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14.3.3 Chemotherapy or Antineoplastic Drugs. Chemotherapeutic/antineoplastic wastes include spent or excess cytotoxic compounds, liquid and solid waste from cell cultures treated with such agents, expended personal protective equipment, and spill cleanup materials. Some examples of chemotherapy/antineoplastic agents include Adriamycin (doxorubicin), bleomycin, cisplatin, cyclophosphamide, etoposide, fluorouracil, and mitomycin C. Chemotherapeutic wastes that are mingled with regulated medical (biohazardous) wastes are classified as chemotherapeutic wastes (RCRA) and must be managed as such. Chemotherapeutic/antineoplastic wastes must be disposed separately from regulated medical or regular municipal waste according to federal and state regulations. See instructions on disposal of chemical (RCRA) hazardous waste. Solid chemotherapeutic wastes cannot be disposed in the regular trash or in biohazard bags. Liquid chemotherapeutic wastes cannot be disposed down the drain or sanitary sewer. 14.4 Disposal Methods.

14.4.1 Sanitary Sewer. The only acceptable amounts of hazardous waste to be put down the drain are those commonly associated with washing of laboratory glassware. Never pour any chemicals down the drains. 14.4.2 Ordinary Trash. Under no circumstances can any chemical be discarded in the regular trash. Containers that are empty and no more that 2.5 cm of residue remain on the bottom of the container can be triple rinsed, original label marked out and placed outside the laboratory for disposal by the Housekeeping staff. Empty containers of acutely hazardous chemicals must be triple rinsed with an appropriate solvent prior to disposal. The waste solvent must be disposed of as hazardous waste. An alternative is to dispose of the container as hazardous waste. Please contact the Environmental Protection Division for help in handling this waste. When work is to be done with highly toxic materials which pose a significant threat to human health or the environment contact the Environmental Health & Safety office prior to beginning the work so that appropriate disposal plan can be arranged. Disposal of hazardous chemical waste that meets any of the definitions of “hazardous” will be picked up by Environmental Health & Safety for disposal by incineration or other treatment. Guidelines for proper collection, labeling, and storing waste prior to pick-up are given later in this chapter. For the safety of both laboratory personnel and safety staff it is vital that the guidelines be stringently enforced and strictly followed. 14.4.3 Selection of Waste Containers and Packaging. It is the responsibility of each laboratory to provide suitable containers for the collection of its waste. If there are any questions please consult with Environmental Health & Safety to prevent costly errors. If outdated reagents are to be discarded it is appropriate to leave them in their original containers. This is especially true for solid chemicals and small quantities of toxic chemicals. In selecting a waste container, the compatibility of the waste with the container should be the primary concern. Unbreakable containers should be used whenever feasible. Large quantities of flammable liquids should be collected in 2.5 or 5 gallon safety cans. Polyethylene cans are best because of their high resistance to many types of chemicals. It is best not to fill these containers more than 2/3 full. This allows for easier transfer. The five gallon plastic jugs and metal cans in which some solvents are purchased are suitable for one time collection of waste. Use these containers only for the same solvents which they originally held. Continued re-use of these containers is discouraged because they tend to develop leaks with time. Except for safety cans purchased for the collection of solvent waste suitable for bulk disposal, waste containers cannot

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be returned to the laboratories. Do not mix chemicals unless they are mixed in the experiment. Always provide secondary containment when storing chemical waste. All laboratories must adhere to the following statements regarding hazardous chemical waste: a) All containers must be tightly closed with an appropriate cap. Aluminum foil or parafilm are not acceptable caps. b) Evaporation of chemical waste inside of a chemical fume hood is not permitted. c) All containers must be clean on the outside with no evidence of spills or leaks. Dirty or leaking containers may not be picked up. d) Chemically contaminated lab waste (paper towels, diaper pads, etc.) will be treated as hazardous waste. The material should be double bagged and tightly sealed preferably with a knot and labeled appropriately. Do not put broken glass or other sharp items into plastic bags. These must be placed in a sharps container for disposal. e) Waste chemicals must be collected and stored according to compatibility. f) Liquid waste containers stored in laboratory work areas must not exceed 20 Liters (5 gallons). g) Waste quantities will be subject to the maximum container sizes and type as illustrated in the table below: Flammable Liquids Combustible Liquids Container Type IA IB IC II IIIA Glass 0.5 L 1L 4L 4L 20L

Metal or approved plastic 4L 20L 20L 20L 20L

Safety cans 10L 20L 20L 20L 20L

Metal container (DOT) 4L 20L 20L 227L 227L

Polyethylene (DOT) 4L 20L 20L 227L 227L

Pressurized liquid dispensing 20L 227L 227L 227L 227L container

This table is based on Table 10.1.2 Maximum Allowable Container Capacity, NFPA 45 Standard on Fire Protection for Laboratories Using Chemicals (2011 edition).

14.4.4 Labeling Hazardous Waste Containers. All containers must be appropriately labeled. The label should include the chemical name(s), and the approximate % of each listed component. The words “hazardous waste” must be placed on containers used to collect waste in the laboratories. Incorrect labels must be defaced. See figure 14-1 for an example of an acceptable label:

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Figure 14-1: Hazardous Waste Labels

14.4.5 Campus Collection System. Environmental Health & Safety oversees the collection and disposal of hazardous chemical waste on campus. Waste collected in the laboratories is transported to the appropriate storage facility. Decisions on how to best manage the waste are made based on the type and quantity of waste involved. Information is supplied on the Hazardous Waste for Disposal tags (see Figure 14-2 below) by the generating laboratory. These peel and stick tags must be affixed to all chemical waste containers at the time the chemical waste pick-up is requested. The start date shall be indicated on the tag. The start date reflects the date the request was submitted to Environmental Health & Safety. The Hazardous Waste for Disposal tags must be completely filled out and attached to each container prior to collection by Environmental Health & Safety. All chemicals present in measurable quantities must be listed, even if the substance is not a hazardous waste. For example, water, while not hazardous, is an important constituent of the waste since it may determine the flammability of the mixture. In order to be able to safely dispose of mixtures, all components must be identified. The picture below shows the areas on the tag that must filled out:

Figure 14-2: New Hazardous Waste for Disposal Tags

Investigator Name Start Date (date of request) Chemical Characteristics Total Quantities Disposed

Lab # Chemical Name

% (approximate) Components

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14.4.6 Filling out a Chemical Waste Pickup Request. One Hazardous Waste for Disposal tag should be affixed to each container. Disposal tags provided by Environmental Health & Safety have a peel and stick backing for easy use. Submit your request for chemical waste pick-up by visiting the EH&S website, http://research.uthscsa.edu/safety. All requests should be submitted prior to Tuesdays. Step 1: Click on the Chemical Waste Pickup icon (Under Quick Links).

Step 2: Fill out the requested information. A verification code (displayed on the screen) will appear. Please enter this code to continue with the request.

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Step 3: Submit form. An email is generated and sent to the Environmental Protection Division. Waste is collected on Tuesday for the UTHSCSA main campus, and Thursday or Friday for other satellite campuses (North Campus, Texas Research Park). All waste will be picked up from the laboratories. Do not place any chemical waste in corridors. Special pickups for laboratory cleanouts may be arranged with Environmental Health & Safety.

14.5 Source Reduction / Recycling Hazardous Materials. Hazardous waste generation and disposal is regulated under the Resource Conservation and Recover Act (RCRA). Under these regulations, the UT Health Science Center has a “cradle to grave” responsibility for all hazardous materials generated on our campuses. As a result, the university’s responsibility for these materials begins from the time of delivery to our campuses, and continues even after it has been removed as waste. Disposal of these materials is costly in terms of money, time, and resources. Therefore, reducing, re-using, and recycling hazardous materials is strongly encouraged. Ideally, waste production should be reduced or eliminated. Eliminating the creation of hazardous waste in particular avoids both the management and disposal costs of waste. Reducing waste can reduce or eliminate chemical procurement costs, training requirements, staff and environmental exposure and use of personal protective equipment. When this is not practical or possible, many waste types can be reused or recycled. Environmental Health & Safety currently recycles many wastes generated from laboratory and facilities activities. Below is a list of these efforts:

• Mercury

• Mercury capture system - enables mercury to be removed from waste generated in dental clinics before it goes into the public waste system.

• Thermometer replacement program – we will replace thermometers containing mercury with safer alternatives.

• Circuit boards, color monitors - small amounts of mercury are recovered from circuit boards and color monitors through the state prison system.

• Batteries – all types of batteries are collected for proper recycling. Please submit a Chemical Waste Pick-Up request online at http://research.uthscsa.edu/safety.

• Oil, oil filters, antifreeze – used oil from state vehicles, pumps, and other laboratory equipment.

• PCB ballasts and capacitors –

• Silver – recovered from fixer and developer waste and x-ray film through a silver recovery machine.

• Solvents – materials are bulked and used as secondary fuel.

• Chemical Exchange Program - labs can exchange excess chemical rather than dispose of them. Please visit the ‘Chemical Exchange Program.’ at the Environmental Health & Safety website. More information on this program is discussed in section 14.6 below.

14.6 Chemical Exchange Program. The Chemical Exchange Program is a free service provided by Environmental Health and Safety which allows laboratory personnel to remove new, unused chemicals

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which are no longer in use in their lab. The advantage of this program is to reduce purchasing and disposal costs and to be good environmental stewards. This program is one of many ways in which UTHSCSA can support the Campus Green Initiative and be a strong proponent of environmental best management practices through waste minimization. Once a chemical exchange form has been submitted, it will be logged into a database for researchers and lab personnel to view. If there is a chemical you need, call 567-2955 for delivery. Chemical(s) will only be delivered on Tuesdays. Below are the steps to take to participate in this program:

1. Review your chemical inventory and identify any chemicals you would like to dispose. Containers must be new, never opened, contains the original packaging seal, and meets EH&S requirements listed below.

2. The Chemical Exchange Program will NOT accept the following:

Controlled substance

Peroxide Formers (e.g. Ethyl ether [diethyl ether], Cyclohexane, Sodium amide)

P-Listed Chemicals (e.g. Sodium Azide, Osmium Tetroxide, Epinepehrine, or anything containing Arsenic or Cyanide)

Pyrophorics (e.g. Phosphorus [white], tert-Butyllithium, or any reactive materials)

Mercury in any form

Expired chemicals

Open or broken sealed containers

Radioactive materials

Materials requiring refrigeration or special handling

Rusted or deteriorated containers

3. If all requirements are met, click on the Chemical Exchange Program icon (Under Quick Links on the EHS website).

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Complete the information and submit to Environmental Health & Safety.

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15.0 Chemical Spills and Emergency Response 15.1 Emergency Plans. In any emergency situation, it is essential to be familiar with response procedures. UTHSCSA’s Emergency Response and Evacuation Plan is available on the Environmental Health & Safety website, http://research.uthscsa.edu/safety/evacplans.shtml, and covers procedures for several different emergency scenarios. Principal Investigators and supervisors should have lab-specific procedures for responding to various types of emergencies. It is highly recommended that you conduct periodic exercises so personnel become familiar with these procedures. This chapter outlines several emergency response procedures that may occur in labs. 15.2 Reporting a Chemical Spill. Please call 567-2955 during business hours and 567-2800 after hours and provide the following information:

1. Your Name 2. Phone Number where you can be reached 3. Location of spill (building, room number, corridor, etc.) 4. Time of spill 5. Any injuries? If so, has individual been attended to? 6. Chemical spilled? 7. Quantity spilled? 8. Powder or liquid? 9. Broken glassware? 10. Area Secured?

15.2.1 Chemical Spill Response and Decontamination Procedures.

Small spills of low hazard chemicals (less than 1 liter), and you are able to safely clean up the spill without assistance:

1. Notify other workers in the area of the spill and control traffic through area. If a spill occurs in the public corridor, contact UT Police for assistance in traffic control (7-2800). 2. Remove any contaminated clothing.

3. Utilize nearest emergency safety shower or eyewash station. 4. Put on gloves, shoe covers and cover spill with appropriate material.

5. Carefully pour appropriate absorbent material/neutralizing agent over spill. 6. Recover spill materials with broom, squeegee and dustpan and place in a waste bag.

7. Pick up any broken glass with forceps and dispose of in a sharps container. 8. Wipe area clean.

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9. Remove gloves and shoe covers before leaving area and place in waste bag.

10. Secure waste bag and label appropriately. This should be disposed of as hazardous

waste. Contact Environmental Health & Safety (http://research.uthscsa.edu/safety/index.shtml) to request a pick-up.

Large spills (> 1 liter) of any chemical, or any amount of acutely hazardous, highly flammable, explosive, toxic or poison, any unknown substance, or if you are uncomfortable with the situation:

1. Evacuate room, close door, and prevent others from entering. 2. Contact Environmental Health & Safety, 567-2955, for assistance. Be prepared to

provide the following information: location of spill, time of spill, name of chemical, physical form (powder, liquid, etc.), quantity, injured persons, security of area where spill occurred.

15.3 Post Exposure/Injury. If a spill results in an exposure (i.e. inhalation, skin contact, eye contact, or ingestion):

1. Seek medical attention as soon as possible. Be prepared to provide the physician with information about the chemical:

2. During normal working hours, call the Employee Health & Wellness Clinic (210-567-2788).

3. After normal working hours, contact University Hospital, Emergency Room (210-358-2488). This location is experienced with laboratory hazardous exposures.

4. Report incident to your supervisor as soon as possible, and with your supervisor, complete the Employer’s First Report of Injury form available in the UTHSCSA Human Resources website.

5. Report all chemical incidents to Environmental Health & Safety at 210-567-2955.

It is important to fill out all of the appropriate paperwork in order to be eligible to collect workers compensation should any illnesses arise from the hazardous exposure in the future. 15.4 Recommended First Aid Procedures. The following table outlines general procedures to be followed in the event of a chemical accident. Always treat the most urgent symptom first: (1) Cessation of breathing. (2) Eye injury. (3) Skin contact. (4) Shock.

Symptom Signs Treatment Cessation of breathing

Get medical help immediately; call UT Police, 7-2800. While waiting for medical help, administer CPR if you are certified.

Spills, splashes

Redness, burning sensation, or general irritation of exposed skin, eyes.

Flush exposed areas of the body with large quantities of cool water for at least 15 minutes. Use eyewash station or safety showers. Lab sinks may be used for washing smaller areas.

Ingestion Discoloration of lips or Identify the chemical.

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mouth Poison or unusual odor of the breath Burning in the throat Unconsciousness Confusion Sudden illness

Follow first aid instructions on MSDS or container label. Do not induce vomiting if victim is unconscious or if the throat is burning. Call the South Texas Poison Control Center 1-800-222-1222. Call UT Police, 7-2800 or 911.

Symptom Signs Treatment Inhalation Confusion

Burning in the throat or lungs Dizziness Unconsciousness

Remove victim to fresh air If victim is not breathing, administer CPR (if certified). Call UT Police, 7-2800 or 911. Consult a physician.

Shock Cold, clammy skin Perspiration on forehead, palms Paleness Chills Nausea Shallow breathing

Keep victim lying down and warm. Elevate legs if possible. Control bleeding as needed. Keep airway open.

Needle punctures

Break in the skin

Identify the hazardous material. Determine quantity in syringe if possible. Contact a physician. Report to EH&S, 7-2955.

15.5 Fire Prevention Procedures. Special considerations should be made to reduce the potential for fires in laboratories. Routine evaluation of lab activities may help to identify conditions in which a fire may occur. Preventative measures should be implemented to reduce the risk of a fire. Below are some conditions to consider: Handling and storage of chemicals, flammable and combustible liquids, and gases: - keep away from heat sources or open flames - store only small quantities of flammable liquids on work benches - keep large volume stock bottles in a chemical fume hood or flammable storage cabinet - Adequately label all containers with the chemical name and hazard identifier (to review labeling requirement, see Chapter 3 of this handbook) - segregate flammables from acids and oxidizers Open flame and spark-producing equipment: - use extreme caution when working with open flame devices - never leave an open flame unattended - routinely check the condition of tubing and replace when cracks or damage is visible - keep combustible materials (paper, plastic) away from open flames - make sure gas valve is turned off when finished

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Use of extension cords / surge protectors / power strips: - use only in emergency situations - do not overload circuits; verify amperage output and do not exceed - use devices that are UL listed and are equipped with a circuit breaker - secure cords to minimize tripping hazards 15.5.1 Responding to Fire Emergencies In the event of a fire, follow the RACE acronym:

R eact to the situation – Use a campus phone to call 911 (UTPD) or a cell phone to call 567-8911 – Stay calm and answer all the dispatcher’s questions A ctivate nearest fire alarm pull station. Inform your emergency evacuation coordinator or supervisor of fire C lose doors behind you as you leave E xit building using nearest, safe exit. DON’T USE ELEVATORS!

Inform your emergency evacuation coordinator or supervisor of anyone needing assistance. Assemble with your work group at least 300 feet from the building at your predetermined meeting place. Perform a head count and inform your emergency evacuation coordinator and supervisor of any missing individuals. Wait for the all-clear signal to re-enter the building – the all-clear signal will be given by 1) UTPD, 2) San Antonio Fire Department, or 3) Environmental Health & Safety. 15.5.2 How to Extinguish Clothing Fires. Clothing fires may occur in laboratories as a result of an accident or an actual fire in the lab. It is imperative that laboratory personnel become familiar with proper procedures for extinguishing clothing fires. If your clothing catches on fire, remember to STOP, DROP, AND ROLL: STOP – do not move; DO NOT RUN. DROP – immediately drop to the floor. ROLL - smother the flames by rolling back and forth until the flames are extinguished. If you see someone whose clothing has caught on fire, you may need to assist them with the above procedures. Many times people will panic and run if clothing ignites, which can results in more severe injuries.

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16.0 Decommissioning Laboratories 16.1 Purpose. The Laboratory Decommissioning and Clearance Policy is designed to assist Principal Investigators who are departing from the Health Science Center, or who are re-locating to other laboratory space within the Health Science Center. The purpose of this policy is to ensure that the laboratory is free of all hazardous materials upon departure or re-assignment of laboratory space. When laboratories are vacated, all chemical, radioactive and biological materials, sharps and other wastes must be removed, transferred or disposed of properly. Failure to take responsibility for chemicals, radioactive, or biological materials can create unsafe and non-compliant conditions. Hazardous materials left behind can be expensive and time consuming to manage. Therefore, it is imperative that the individual who is most knowledgeable about the materials used, stored, or disposed in a research laboratory complete the attached form. Compliance with this policy will allow Environmental Health & Safety to deactivate vacated laboratory space for re-assignment to new occupants. All Principal Investigators, Department Chairs, and laboratory or clinical managers should become familiar with the conditions set forth in this policy. Additional information regarding laboratory hazards may be obtained through the Environmental Health & Safety’s Chemical, Biological, Radiological, and Physical Safety Handbooks. 16.2 Responsibilities. 16.2.1 Department Chair - must ensure that this policy is distributed to all Principal Investigators in their department. The Department Chair must notify Environmental Health and Safety if a Principal Investigator is leaving the Health Science Center, transferring their laboratory to another location or closing down a laboratory. Ultimate responsibility for hazardous materials management lies with each department. 16.2.2 Principal Investigators - when a Principal Investigator vacates or closes down a laboratory, they must contact their department Chair and Environmental Health and Safety to begin the decommissioning process. The laboratory must be left in a condition which is safe for the next occupant, or for construction/renovation workers. It is the responsibility of the Principal Investigator to ensure that all hazardous materials in use in the laboratory are disposed of according to the policies of the Health Science Center. 16.2.3 Environmental Health & Safety - is responsible for verifying that safety hazards have been removed from the laboratory before Housekeeping, Facilities Management, or any other Principal Investigator enters the laboratory. Environmental Health & Safety will verify that the laboratory was left in a safe condition and post a notice verifying that it is safe to clean, renovate, or move into the laboratory. 16.3 Laboratory Decommissioning Procedure. The following procedure must be implemented by any Principal Investigator, researcher, instructor, laboratory or clinical manager who is decommissioning a laboratory or laboratory area prior to leaving the Health Science Center, relocating to another University laboratory, or renovating their laboratory. This also applies to department chairs in the event of the death, disability or termination of the previously mentioned personnel under their responsibility. 1. At least thirty (30) days prior to the expected date of vacating the laboratory or laboratory space, the principal investigator, researcher, instructor, laboratory or clinical

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manager must submit written notification of their intended plans to leave the Health Science Center, renovate the laboratory and/or relocate to another Health Science Center laboratory.

2. All laboratories (chemical, biological, and radiological) must be cleared by Environmental Health & Safety prior to relocating or departing from the Health Science Center:

A. Chemicals - All chemicals, including waste chemicals, must be removed from the laboratory. If chemicals are to be transferred to another Principal Investigator located on same campus as the decommissioning laboratory, please submit these changes to Environmental Health & Safety. Any remaining unwanted chemicals must be disposed of through Environmental Health & Safety. No chemicals can be removed from the campus without prior approval of the Office of Environment Health & Safety. Empty containers must be triple rinsed and labels defaced prior to disposal in the regular trash. Hazardous waste or unwanted chemicals must be disposed of as per Environmental Health & Safety procedures by submitting an on-line chemical waste pickup request at http://research.uthscsa.edu/safety.

Controlled substances (Schedule I – V regulated through Drug Enforcement Agency (DEA)) shall be disposed of through UT Police. Contact UTPD at 567-2800 for information regarding Controlled Substance Disposal dates. B. Radioactive Materials - All radioisotopes must be disposed of in the appropriate waste containers per the Radiation Safety Handbook Waste Management section. The radioisotopes must be removed from the laboratory through a Radiation Waste Pickup by contacting Environmental Health & Safety through the on-line radiation waste pickup request, http://research.uthscsa.edu/safety. The Principal Investigator must contact the Radiation Safety Division and complete the inventory verification form prior to leaving the Health Science Center to show that all radioisotopes have been removed from their inventory. If radioactive materials will be transferred to another Principal Investigator, contact the Radiation Safety Division for approval. All laboratory areas will be wipe tested by Environmental Health & Safety for contamination. If contamination is present, the area must be decontaminated to remove all radioactive contamination. The area should be re-wipe tested until counts are below 1000 dpm/100 cm2. C. Biological Materials - Biological safety cabinets may require decontamination. Contact Environmental Health and Safety to schedule these services, by submitting an on-line request for a Biosafety Cabinet Decontamination at http://research.uthscsa.edu/safety. Biological safety cabinets that are moved must be re-certified prior to use. Contact Environmental Health & Safety for assistance with disposal of biohazardous material (biological toxins, cultures, frozen stocks, tissues, etc.) by chemical destruction, autoclaving or incineration as appropriate. Contact Environmental Health & Safety for destruction of Select Agents & Toxins.

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Clean and disinfect all laboratory surfaces and equipment with an approved disinfecting agent. Ensure proper contact time for adequate deactivation of biohazardous agents.

D. All laboratory equipment (centrifuges, freezers, refrigerators, etc.) must be cleared by Environmental Health & Safety prior to being moved. Most moving companies require clearance from Environmental Health & Safety. If equipment was used to store or process radioactive material, the Radiation Safety Division will perform wipe tests. Before submitting an Equipment Clearance Request, please read the requirements below:

Make sure all chemical, biological, and radiological elements have been removed from item to be cleared (including pump oil). Make sure all lab supplies (tubes, vials, etc.) have been removed from the unit. All items are to be cleaned thoroughly and wiped down with 10% Bleach (freshly-prepared), 70% Ethanol, or another approved disinfectant (i.e. Bleach Rite). If the unit to be cleared is a refrigerator or freezer, the unit must be unplugged, defrosted, and wiped dry. Please inform Environmental Health & Safety if samples are to remain during the move. Requests to clear equipment may be submitted online at http://research.uthscsa.edu/safety.

E. All surplus controlled glassware and laboratory apparatus shall be safely destroyed and placed in a sturdy cardboard boxed labeled “Broken Glassware”. Housekeeping personnel will removed all broken glassware boxes from the labs. For assistance, contact Environmental Health & Safety (567-2955).

3. Before submitting a Lab Clearance Request, please read the requirements below:

Make sure all chemical, biological, and radiological elements (unwanted chemicals, radioactive waste, regulated medical waste boxes) have been removed from the lab. Make sure all laboratory equipment has been cleared and that storage locations have been secured (if applicable). Make sure all drawers and cabinets have been emptied. The laboratory must be completely emptied before Environmental Health & Safety will clear it. Requests to clear labs may be submitted online, at http://research.uthscsa.edu/safety. Labs will be cleared and decommissioned upon completion of a final walk-through of the laboratory. The Principal Investigator must ensure completion of the Laboratory Decommissioning Procedures Checklist (Appendix H). Additionally, all parties (Principal Investigator, Department Chair or designee, and Environmental Health & Safety) must sign the Laboratory Decommissioning Procedures Checklist.

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17.0 RECORD KEEPING

17.1 Record Keeping Requirements for Laboratories. All laboratories using hazardous chemicals are required to keep copies of records as follows: 17.1.1 Chemical Safety Handbook. All labs shall have a copy of the most current version of the Chemical Safety Handbook accessible to all lab personnel. 17.1.2 Safety Data Sheets (SDS). SDS will be made available to all employees electronically or by hard copy if electronic access is unavailable. MSDS will be available until the chemical is no longer stored at UTHSCSA. 17.1.3 Chemical Inventory. Chemical inventories are required to be updated annually. Copies of chemical inventories will be kept in the lab for 30 years. 17.1.4 Laboratory Safety Evaluations. Records of safety evaluations performed by Environmental Health & Safety will be kept of file for a minimum of 5 years. Records of self-evaluations should also be maintained in the laboratory.

17.1.5 Employee Training. Copies of all training records will be kept by lab personnel. Training provided by Environmental Health & Safety will be documented in the form of a training certificate, with the name of the employee, title of training class, date of attendance, and topics of discussion. This documentation will be maintained in the laboratory. Principal investigators are required to maintain training records for 5 years. 17.1.6 Chemical Exposure Assessments. Results from these assessments will be posted in a conspicuous place in the laboratory. A copy of the results will also be kept in the chemical safety handbook. These records will be kept permanently.

17.2 Record Keeping Requirements for Environmental Health & Safety Office.

17.2.1 Hazard Communication Program. All Hazard Communication Plans will be kept on file for 5 years beyond supersede date. 17.2.2 Chemical Safety Handbooks. Revisions of the Chemical Safety Handbook will be kept on file for 5 years past supersede date.

17.2.3 Tier Two Report. Tier Two Reports will be kept permanently in Environmental Health & Safety Office.

17.2.4 Safety Data Sheets (SDS). Electronic database of the most current MSDS for each hazardous chemical housed at UTHSCSA will be maintained by Environmental Health & Safety. MSDS will be available until the chemical is no longer stored at UTHSCSA. 17.2.5 Chemical Inventories. Annual chemical inventory updates will be kept in the Environmental Health & Safety Office for 30 years.

17.2.6 Laboratory Safety Evaluations. Safety evaluations will be kept on file for 5 years after all deficiencies have been corrected.

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17.2.7 Employee Training. Training records will be kept on file for 5 years.

17.2.8 Chemical Exposure Assessments. All chemical exposure assessments will be kept permanently.

17.2.9 Incident Reports. All reports generated by Environmental Health & Safety regarding spills/injuries at UTHSCSA will be kept for 10 years.

17.2.10 Records of Terminated Principal Investigators. Records of terminated Principal Investigators (PI's) Includes correspondence related to safety issues, surveys performed in their laboratories, spills/injuries that may have occurred, and shall be kept for 10 years past termination date.

Appendix A: TDSHS Notice to Employees

Appendix B: Chemical Carcinogens Regulated by Occupational Safety and Health Administration (OSHA). Chemical Name CAS Number 1,2-Dibromo-3-chloropropane 96-12-8 1,3-Butadiene 106-99-0 2-Acetylaminofluorene 53-96-3 3,3’-Dichlorobenzidine 91-94-1 4-Aminodiphenyl 92-67-1 4-Dimethylaminoazobenzene 60-11-7 4-Nitrobiphenyl 92-93-3 Acrylonitrile 107-13-1 Alpha-Naphthylamine 134-32-7 Asbestos 1332-21-4 Benzene 71-43-2 Benzidine 92-87-5 Beta-Naphthylamine 91-59-8 Beta-Propiolactone 57-57-8 Bis-Chloromethyl Ether 542-88-1 Cadmium 7440-43-9 Coke oven emissions Ethylene Oxide 75-21-8 Ethyleneinine 151-56-4 Formaldehyde 50-00-0 Inorganic Arsenic 7440-38-2 Methyl Chloromethyl Ether 107-30-2 Methylene Chloride 75-09-2 Methylenedianiline 101-77-9 N-Nitrosodimethylamine 62-75-9 Vinyl Chloride 75-01-4

Appendix C: Substances Known To Be Human Carcinogens National Toxicology Program, Department of Health and Human Services Report on Carcinogens, Twelfth Edition (2011) Bold entries indicate new or changed listings in the Twelfth Report on Carcinogens. Aflatoxins Alcoholic Beverage Consumption 4-Aminobiphenyl Analgesic Mixtures Containing Phenacetin (see Phenacetin and Analgesic Mixtures Containing

Phenacetin) Aristolochic Acids Arsenic and Inorganic Arsenic Compounds Asbestos Azathioprine Benzene Benzidine (see Benzidine and Dyes Metabolized to Benzidine) Beryllium and Beryllium Compounds Bis(chloromethyl) Ether and Technical-Grade Chloromethyl Methyl Ether 1,3-Butadiene Cadmium and Cadmium Compounds Chlorambucil 1-(2-Chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea (see Nitrosourea Chemotherapeutic Agents) Chromium Hexavalent Compounds Coal Tars and Coal-Tar Pitches Coke-Oven Emissions Cyclophosphamide Cyclosporin A Diethylstilbestrol Dyes Metabolized to Benzidine (Benzidine Dye Class) (see Benzidine and Dyes Metabolized to

Benzidine) Erionite Estrogens, Steroidal Ethylene Oxide Formaldehyde Hepatitis B Virus Hepatitis C Virus Human Papillomaviruses: Some Genital-Mucosal Types Melphalan Methoxsalen with Ultraviolet A Therapy Mineral Oils: Untreated and Mildly Treated Mustard Gas 2-Naphthylamine Neutrons (see Ionizing Radiation) Nickel Compounds (see Nickel Compounds and Metallic Nickel) Radon (see Ionizing Radiation) Silica, Crystalline (Respirable Size) Solar Radiation (see Ultraviolet Radiation Related Exposures)

Soots Strong Inorganic Acid Mists Containing Sulfuric Acid Sunlamps or Sunbeds, Exposure to (see Ultraviolet Radiation Related Exposures) Tamoxifen 2,3,7,8-Tetrachlorodibenzo-p-dioxin Thiotepa Thorium Dioxide (see Ionizing Radiation) Tobacco Smoke, Environmental (see Tobacco-Related Exposures) Tobacco Smoking (see Tobacco-Related Exposures) Tobacco, Smokeless (see Tobacco-Related Exposures) Ultraviolet Radiation, Broad-Spectrum (see Ultraviolet Radiation Related Exposures) Vinyl Chloride (see Vinyl Halides [selected] Wood Dust X-Radiation and Gamma Radiation (see Ionizing Radiation

Appendix D: International Agency for Research on Cancer, last updated 13 July, 2013.

Group 1: Carcinogenic to Humans

Acetaldehyde (from consuming alcoholic beverages) Acid mists, strong inorganic Aflatoxins Alcoholic beverages Aluminum production 4-Aminobiphenyl Areca nut Aristolochic acid (and plants containing it) Arsenic and inorganic arsenic compounds Asbestos (all forms) and mineral substances (such as talc or vermiculite) that contain asbestos Auramine production Azathioprine Benzene Benzidine and dyes metabolized to benzidine Benzo[a]pyrene Beryllium and beryllium compounds Betel quid, with or without tobacco Bis(chloromethyl)ether and chloromethyl methyl ether (technical-grade) Busulfan 1,3-Butadiene Cadmium and cadmium compounds Chlorambucil Chlornaphazine Chromium (VI) compounds Clonorchis sinensis (infection with) Coal, indoor emissions from household combustion Coal gasification Coal-tar distillation Coal-tar pitch Coke production Cyclophosphamide Cyclosporine Diethylstilbestrol Engine exhaust, diesel Epstein-Barr virus (infection with) Erionite Estrogen postmenopausal therapy Estrogen-progestogen postmenopausal therapy (combined) Estrogen-progestogen oral contraceptives (combined) (Note: There is also convincing evidence in humans that these agents confer a protective effect against cancer in the endometrium and ovary) Ethanol in alcoholic beverages Ethylene oxide Etoposide Etoposide in combination with cisplatin and bleomycin

Fission products, including strontium-90 Formaldehyde Haematite mining (underground) Helicobacter pylori (infection with) Hepatitis B virus (chronic infection with) Hepatitis C virus (chronic infection with) Human immunodeficiency virus type 1 (HIV-1) (infection with) Human papilloma virus (HPV) types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 (infection with) (Note: The HPV types that have been classified as carcinogenic to humans can differ by an order of magnitude in risk for cervical cancer) Human T-cell lymphotropic virus type I (HTLV-1) (infection with) Ionizing radiation (all types) Iron and steel founding (workplace exposure) Isopropyl alcohol manufacture using strong acids Kaposi sarcoma herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) (infection with) Leather dust Magenta production Melphalan Methoxsalen (8-methoxypsoralen) plus ultraviolet A radiation 4,4'-Methylenebis(chloroaniline) (MOCA) Mineral oils, untreated or mildly treated MOPP and other combined chemotherapy including alkylating agents 2-Naphthylamine Neutron radiation Nickel compounds N'-Nitrosonornicotine (NNN) and 4-(N-Nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) Opisthorchis viverrini (liver fluke; infection with) Painter (workplace exposure as a) 3,4,5,3',4'-Pentachlorobiphenyl (PCB-126) 2,3,4,7,8-Pentachlorodibenzofuran Phenacetin (and mixtures containing it) Phosphorus-32, as phosphate Plutonium Radioiodines, including iodine-131 Radionuclides, alpha-particle-emitting, internally deposited (Note: Specific radionuclides for which there is sufficient evidence for carcinogenicity to humans are also listed individually as Group 1 agents) Radionuclides, beta-particle-emitting, internally deposited (Note: Specific radionuclides for which there is sufficient evidence for carcinogenicity to humans are also listed individually as Group 1 agents) Radium-224 and its decay products Radium-226 and its decay products Radium-228 and its decay products Radon-222 and its decay products Rubber manufacturing industry Salted fish (Chinese-style) Schistosoma haematobium (flatworm; infection with) Semustine (methyl-CCNU) Shale oils

Silica dust, crystalline, in the form of quartz or cristobalite Solar radiation Soot (as found in workplace exposure of chimney sweeps) Sulfur mustard Tamoxifen (Note: There is also conclusive evidence that tamoxifen reduces the risk of contralateral breast cancer in breast cancer patients and lowers the risk of breast cancer in women at increased risk) 2,3,7,8-Tetrachlorodibenzo-para-dioxin Thiotepa Thorium-232 and its decay products Tobacco, smokeless Tobacco smoke, secondhand Tobacco smoking ortho-Toluidine Treosulfan Ultraviolet (UV) radiation, including UVA, UVB, and UVC rays Ultraviolet-emitting tanning devices Vinyl chloride Wood dust X- and Gamma-radiation

Group 2A: Probably Carcinogenic to Humans

Acrylamide Adriamycin Androgenic (anabolic) steroids Azacitidine Benz[a]anthracene BCNU (Bischloroethyl nitrosourea) Captafol Chloramphenicol α-Chlorinated toluenes (benzal chloride, benzotrichloride, benzyl chloride) and benzoyl chloride (combined exposures) CCNU (1-(2-Chloroethyl)-3-cyclohexyl-1-nitrosourea) 4-Chloro-ortho-toluidine Chlorozotocin Cisplatin Cyclopenta[cd]pyrene Clonorchis sinensis (infection with) Dibenz[a,h]anthracene Dibenzo[a,l]pyrene Diethyl sulfate Dimethylcarbamoyl chloride 1,2-Dimethylhydrazine Dimethyl sulfate Epichlorohydrin Ethylene dibromide Ethyl carbamate (urethane)

N-Ethyl-N-nitrosourea Etoposide Glycidol Indium phosphide 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) Lead compounds, inorganic Kaposi's sarcoma herpesvirus/human herpesvirus 8 5-Methoxypsoralen Methyl methanesulfonate MNNG (N-Methyl-N'-nitro-N-nitrosoguanidine) N-Methyl-N-nitrosourea Nitrate or nitrite (ingested) under conditions that result in endogenous nitrosation Nitrogen mustard N-Nitrosodiethylamine (DEN) N-Nitrosodimethylamine (NDMA) Phenacetin Procarbazine hydrochloride Styrene-7,8-oxide Teniposide Tetrachloroethylene Trichloroethylene 1,2,3-Trichloropropane Tris(2,3-dibromopropyl) phosphate Ultraviolet radiation A Ultraviolet radiation B Ultraviolet radiation C Vinyl bromide Vinyl fluoride

Appendix E: EPA "P" LIST ACUTELY HAZARDOUS WASTE CHEMICAL NAME CAS# EPA# Acetyl-2-thiourea 591082 P002 Acrolein 107028 P003 Aldicarb 116063 P070 Aldrin 309002 P004 Allyl alcohol 107186 P005 Aluminum phosphide 20859738 P006 Aminomethyl)-3-isoxazolol, 5-( 2763964 P007 Aminopyridine, 4- 504295 P008 Ammonium ferrocyanide 14481299 P030 Ammonium ferricyanide 14221488 P030 Ammonium picrate 131748 P009 Ammonium vanadate 7803556 P119 Arsenic acid 7778394 P010 Arsenic trioxide 1327533 P012 Arsenic pentoxide 1303282 P011 Barium cyanide 542621 P013 Benzenethiol 108985 P014 Benzyl chloride 100447 P028 Beryllium powder 7440417 P015 Bromoacetone 598312 P017 Brucine 357573 P018 Calcium cyanide 592018 P021 Calcium cyanamide 156627 P030 Carbon disulfide 75150 P022 Chloroacetaldehyde 107200 P023 Chloroanaline, p- 106478 P024 Chloropropionitrile, 3- 542767 P027 Chlororphenyl)-2-thiourea, 1-(2- 5344821 P026 Cupric cyanide 14763770 P029 Cuprous cyanide 544923 P029 Cyanamide, calcium salt 156627 P030 Cyanogen 460195 P031 Cyanogen chloride 506774 P033 Cyanuric chloride 108770 P030 Dichloromethyl ether 542881 P016 Dichlorophenylarsine 696286 P036 Dieldrin 60571 P037 Diethyl p-nitrophenyl phosphate 311455 P041

CHEMICAL NAME CAS# EPA# Diethyl o-pyrazinyl phosphoro- thioate 297972 P040 Diethylarsine 692422 P038 Diisopropylfluorophosphate 55914 P043 Dimethoate 60515 P044 Dimethylphenethylamine 19342019 P046 Dinito-o-cresol, 4,6- 534521 P047 Dinitro-o-cyclohexylphenol, 4,6- 131895 P034 Dinitrophenol, 2,4 51285 P048 Dinoseb 88557 P020 Disulfoton 298083 P039 Dithiobiuret, 2,4- 32976888 P049 Endosulfan 115297 P050 Endothal 145733 P088 Endrin 72208 P051 Epinephrine 51434 P042 Ethyleneimine 151564 P054 Famphur 52857 P097 Ferric ferrocyanide 14038438 P030 Fluorine (gas) 7782414 P056 Fluoroacetamide 640197 P057 Heptachlor 76448 P059 Hexamethyl tetraphosphate 757584 P062 Hydrocyanic acid 74908 P063 Isodrin 465736 P060 Mercuric cyanide 592041 P030 Mercuric oxycyanide 1335315 P030 Mercury fulminate 628864 P066 Methyl isocyanate 624839 P064 Methyl pararthion 298000 P071 Methylaziridine, 2, 75558 P067 Methylhydrazine 60344 P068 Methyllactonitrile, 2- 75865 P069 Naphthylthiourea, alpha- 86884 P072 Nickel cyanide 557197 P074 Nickel carbonyl 13463393 P073 Nicotine, L- 54115 P075 Nitric oxide 10102439 P076 Nitroanaline, p- 100016 P077 Nitrogen dioxide 10102440 P078 Nitroglycerine 55630 P081

CHEMICAL NAME CAS# EPA# Nitrosodimethylamine 62759 P082 Nitrosomethylvinylamine, N 4549400 P084 Octamethylpyrophosphoramide 152169 P085 Osmium tetraoxide 20816120 P087 Parathion 56382 P089 Phenyl-2-thiourea, 1- 103855 P093 Phenylmercuric acetate 62384 P092 Phorate 298022 P094 Phosgene 75445 P095 Phosphine 7803512 P096 Potassium silver cyanide 506616 P099 Potassium ferricyanide 13746662 P030 Potassium cyanide 151508 P098 Potassium ferrocyanide trihydrate 14459951 P030 Propargyl alcohol 107197 P102 Propionitrile 107120 P101 Selenourea 630104 P103 Silver cyanide 506649 P104 Sodium ferrocyanide 13601199 P030 Sodium nitroferricyanide 14402892 P030 Sodium fluoroacetate 62748 P058 Sodium cyanide 143339 P106 Sodium azide 26628228 P105 Strychnine, L- 57249 P108 Tetraethyl dithiopyrophosphate 3689245 P109 Tetraethyl pyrophosphate 107493 P111 Tetraethyl lead 78002 P110 Tetranitromethane 509148 P112 Thallium selenite 12039520 P114 Thallium sulfate 7446186 P115 Thallium (III) oxide 1314325 P113 Thiofanox 39196184 P045 Thiosemicarbazide 79196 P116 Toxaphene 8001352 P123 Trichloromethanethiol 75707 P118 Vanadium pentoxide 1314621 P120 Warfarin 81812 P001 Zinc cyanide 557211 P121 Zinc phosphide 1314847 P122

Appendix F: EPA "U" LIST TOXIC HAZARDOUS WASTE CHEMICAL NAME CAS# EPA# Acetaldehyde 75070 U001 Acetone 67641 U002 Acetonitrile 75058 U003 Acetyl chloride 75365 U006 Acetylaminofluorene, 2- 53963 U005 Acrylamide 79061 U007 Acrylic acid 79107 U008 Acrylonitrile 107131 U009 Actophenone 98862 U004 Amitrole 61825 U011 Aniline oil 62533 U012 Auramine 492808 U014 Azaserine 115026 U015 Benzal chloride 98873 U017 Benzanthrene, 1,2 56553 U018 Benzenamine 62533 U012 Benzene sulfonyl chloride 98099 U020 Benzene 71432 U019 Benzenediol, 1,3- 108463 U201 Benzidine 92875 U021 Benzoquinone, p- 106514 U197 Benzotrichloride 98077 U023 Benzo[a]pyrene 50328 U022 Benz[c]acridine 225514 U016 Bioxirane, 2,2'- 1464535 U085 Bis (2-chloroethyl)ether 111444 U025 Bis (2-ethylhexyl)phthalate 117817 U028 Bis (2-chloroethoxy)methane 111911 U024 Bis (2-chloroisopropyl)ether 108601 U027 Bromoform 75252 U225 Bromophenyl ether, 4- 101553 U030 Butanone, 2- 78933 U159 Butyl phthalate, N- 84742 U069 Butyl alcohol 71363 U031 Cacodylic acid 75605 U136 Calcium chromate 8012757 U032 Carbon tetrachloride 56235 U211 Carbonyl fluoride 353504 U033

CHEMICAL NAME CAS# EPA# Chlorambucil 305033 U035 Chlordane 57749 U036 Chlornaphazine 494031 U026 Chloro-o-toluidine hydrochloride 3165933 U049 Chloro-2,3-epoxypropane, 1- 106898 U041 Chloro-m-cresol, 4- 59507 U039 Chlorobenzene 108907 U037 Chloroethane 75014 U043 Chloroethyl vinyl ether 110758 U042 Chloroform 67663 U044 Chloromethyl methyl ether 107302 U046 Chloronaphthalene, 2- 91587 U047 Chlorophenol, o- 95578 U048 Chrysene 218019 U050 Creosote 8001589 U051 Cresol 1319773 U052 Crotonaldehyde 4170303 U053 Cumene 98828 U055 Cyanogen bromide 506683 U246 Cyclohexane 110827 U056 Cyclohexanone 108941 U057 Cyclophosphamide 6055192 U058 Daunomycin 20830813 U059 DDD 72548 U060 DDT 50293 U061 Diallate 2303164 U062 Dianisidine 119904 U091 Dibenzo[a,i]pyrene 189559 U064 Dibenz[a,h]anthrene 53703 U063 Dibromo-3-chloropropane, 1,2- 96128 U066 Dibromoethane, 1,2- 106934 U067 Dibromomethane 74953 U068 Dichloro-2-butene, 1,4- 764410 U074 Dichloro-5,6-dicyano-1,4 benzoquinone 84582 U197 Dichlorobenzene, 1,2- 95501 U070 Dichlorobenzene, 1,3- 541731 U071 Dichlorobenzene, 1,4- 106467 U072 Dichlorobenzidine, 3,3- 91941 U073 Dichlorodifluoromethane 75718 U075 Dichloroethylene, 1,2- 156592 U079 Dichloroethane, 1,2- 107062 U077

CHEMICAL NAME CAS# EPA# Dichloroethane, 1,1- 75343 U076 Dichloroethylene, 1,1- 540590 U078 Dichloromethane 75092 U080 Dichlorophenol, 2,4- 120832 U081 Dichlorophenol, 2,6- 120832 U082 Dichlorophenoxy acetic acid, 2,4- 2307553 U240 Dichloropropane, 1,2- 78875 U083 Dichloropropane, 1,3- 542756 U084 Diepoxybutane 1464535 U085 Diethyl phthalate 84662 U088 Diethyl stilbestrol 56531 U089 Diethyl S-methyl dithiophosphate 3288582 U087 Diethylhydrazine, 1,2- 1615801 U086 Dihydrosafrole 94586 U090 Dimethyl benzyl hydroperoxide 80195 U096 Dimethyl phthalate 131113 U102 Dimethyl sulfate 77781 U103 Dimethylamine 124403 U092 Dimethylbenzidine, 3,3- 119937 U095 Dimethylbenz[a]anthracene, 7,12- 57976 U094 Dimethylcarbamoyl chloride 79447 U097 Dimethylhydrazine, 1,1- 57147 U098 Dimethylhydrazine, 1,2- 540738 U099 Dimethylaminoazobenzene 60117 U093 Dimethylphenol, 2,4- 105679 U101 Dinitrotoluene, 2,4- 121142 U105 Dinitrotoluene, 2,6- 606202 U106 Dioctyl phthalate 117817 U107 Dioxane, 1,4 123911 U108 Diphenylhydrazine, 1,2- 122667 U109 Dipropylamine 142847 U110 Ethylenethiourea 96457 U116 Ethoxyethanol, 2- 110805 U359 Ethyl 4,4'-dichlorobenzilate 510156 U038 Ethyl acetate 141786 U112 Ethyl methacrylate 97632 U118 Ethyl methanesulfonate 62500 U119 Ethyl ether 60297 U117 Ethyl chloride 75003 U043 Ethyl acrylate 140885 U113 Ethyl carbamate 51796 U238 Ethyl glycol monoethyl ether 110805 U359 Ethylene oxide 75218 U115

CHEMICAL NAME CAS# EPA# Ethylenebisdithiocarbamic acid 111546 U114 Fluoranthrene 206440 U120 Formaldehyde 50000 U122 Formic acid 64186 U123 Furaldehyde, 2- 98011 U125 Furan 110009 U124 Glycidyaldehyde 76534 U126 Hexachloro-1,3-butadiene 87683 U128 Hexachlorobenzene 118741 U127 Hexachlorocyclopentadiene 77474 U130 Hexachloroethane 67721 U131 Hexachlorophene 70304 U132 Hexachloropropene 1888717 U243 Hydrazine 302012 U133 Hydrofluoric acid 7664393 U134 Hydrogen sulfide 7783064 U135 Indeno[1,2,3-cd]pyrene 193395 U137 Iodomethane 74884 U138 Isobutyl alcohol 78831 U140 Isopropyl acetone 108101 U161 Isosafrole 120581 U141 Kepone 143500 U142 Lasiocarpine 303344 U143 Lead acetate 301042 U144 Lead phosphate 7446277 U145 Lead subacetate 1335326 U146 Lindane 58899 U129 Maleic anhydride 108316 U147 Maleic acid hydrazide 123331 U148 Malononitrile 109773 U149 Manzate 12427382 U150 Mercury, metallic 7439976 U151 Methacrylonitrile 126987 U152 Methanol 67561 U154 Methapyrilene 91805 U155 Methoxychlor 72435 U247 Methyl methacrylate 80626 U162 Methyl mercaptan 74931 U153 Methyl bromide 74839 U029 Methyl isobutyl ketone 108101 U161

CHEMICAL NAME CAS# EPA# Methyl chloroformate 79221 U156 Methyl ethyl ketone peroxide 1338234 U160 Methyl ethyl ketone 78933 U159 Methyl chloride 74873 U045 Methyl-2-thiouracil, 6- 56042 U164 Methyl-N'-nitro-N- nitrosoguanidine 70257 U163 Methylcholanthrene 56495 U157 Methylene bis (2-chloraniline), 4,4- 101144 U158 Mitomycin-C 50077 U010 Naphthalene 91203 U165 Naphthoquinone, 1,4- 130154 U166 Naphthylamine, alpha- 134327 U167 Naphthylamine, beta- 91598 U168 Nitro-o-toluidine, 5- 99558 U181 Nitrobenzene 98953 U169 Nitrophenol, p- 100027 U170 Nitropropane, 2- 79469 U171 Nitroso-N-ethyl urea, N- 759739 U176 Nitroso-N-methyl urea, N- 684935 U177 Nitroso-N-methyl urethane, N- 615532 U178 Nitrosodi-N-butylamine 924163 U172 Nitrosodi-N-propylamine 621647 U111 Nitrosodiethanolamine, N- 1116547 U173 Nitrosodiethylamine, N- 55185 U174 Nitrosopiperidine, N- 100754 U179 Nitrosopyrrolidine, 1- 930552 U180 Paraldehyde 123637 U182 Pentachlorobenzene 608935 U183 Pentachloroethane 76017 U184 Pentachloronitrobenzene 82688 U185 Pentadiene, 1,3- 504609 U186 Phenacetin 62442 U187 Phenol 108952 U188 Phosphorus sulfide 1314803 U189 Phthalic anhydride 85449 U190 Picoline, 2- 109068 U191 Pronamide 23950585 U192 Propane sultone, 1,3- 1120714 U193 Propylamine 107108 U194 Pyridine 110861 U196 Reserpine 50555 U200

CHEMICAL NAME CAS# EPA# Resorcinol 108463 U201 Saccharin 81072 U202 Safrole 94597 U203 Selenious acid 7783008 U204 Selenium dioxide 7446084 U204 Selenium sulfide 7488564 U205 Streptozotocin 18883664 U206 Tetrachlorobenzene, 1,2,4,5- 95943 U207 Tetrachloroethane, 1,1,1,2- 630206 U208 Tetrachloroethane, 1,1,2,2- 79345 U209 Tetrachloroethylene 127184 U210 Tetrahydrofuran 109999 U213 Thallium (I) carbonate 6533739 U215 Thallium (I) acetate 563688 U214 Thallium (I) nitrate 10102451 U217 Thallium (I) chloride 7791120 U216 Thioacetamide 62555 U218 Thiourea 62566 U219 Thiram 137268 U244 Toluene 108883 U220 Toluene diisocyante, 2,4- 584849 U223 Toluenediamine 25376458 U221 Toluidine, o- 95534 U328 Toluidine hydrochloride, o- 636215 U222 Toluidine, p- 106490 U353 Tribromomethane 75252 U225 Trichloroacetaldehyde 75876 U034 Trichloroethane, 1,1,2- 79005 U227 Trichloroethane, 1,1,1- 71556 U226 Trichloroethylene 79016 U228 Trichlorofluoromethane 75694 U121 Trinitrobenzene, 1,3,5- 99354 U234 Tris (2,3-dibromopropyl) phosphate 126727 U235 Trypan blue 72571 U236 Uracil mustard 66751 U237 Vinyl chloride 75014 U043 Warfarin 81812 U248 Xylenes 1330207 U239 Zinc phosphide 1314847 U249

Appendix G: Toxic Characteristic Constituents and Regulatory Levels Compound Maximum Concentration (mg/liter) Arsenic 5.0 Barium 100.0 Benzene 0.5 Cadmium 1.0 Carbon tetrachloride 0.5 Chlordane 0.03 Chlorobenzene 100.0 Chloroform 6.0 Chromium 5.0 o-Cresol 200.0 m-Cresol 200.0 p-Cresol 200.0 Cresol 200.0 2,4-D 10.0 1,4-Dichlorobenzene 7.5 1,2-Dichloroethane 0.5 2,4-Dichloroethylene 0.7 2,4-Dinitrotoluene 0.13 Endrin 0.02 Heptachlor (and its hydroxide) 0.008 Hexachlorobenzene 0.13 Hexachloro-1,3-butadiene 0.5 Hexachloroethane 3.0 Lead 0.4 Mercury 0.2 Methoxychlor 10.0 Methyl ethyl ketone 200.0 Nitrobenzene 2.0 Pentachlorophenol 100.0 Pyridine 5.0 Selenium 1.0 Silver 5.0 Tetrachloroethylene .07 Toxaphene 0.5 Trichlorethylene 0.5 2,4,5-Trichlorophenol 400.0 2,4,6-Trichlorophenol 2.0 2,4,5-TP 1.0 Vinyl chloride 0.2

Revision 6/9/2011 1

To:

CC: File

From: Rebecca Bloodworth, Chemical Safety Manager

Date:

Re: Annual Hazardous Chemical Inventory Update – 2013

Lab(s):

RETURN TO SAFETY OFFICE (1.343T DTL) BY:

The Texas Hazard Communication Act requires all hazardous chemical inventories be reviewed and updated at least annually. Chemical inventories may be viewed and updated online through the EHS Assistant website, http://ehsaweb.uthscsa.edu/, under Chemical, Inventory. Please review your records and enter appropriate changes (corrections, deletions, additions) and return this signed memo to the Environmental Health & Safety Office, 1.343T DTL, by the date indicated above. Include chemicals with a recognized Physical and/or Health Hazard: Physical Hazards: Combustible, flammable, compressed gas, explosive, organic

peroxide, oxidizer, pyrophoric, or reactive. Health Hazards: Carcinogen, toxic, reproductive hazard, teratogen, irritant, corrosive,

or sensitizer. New Chemicals to be added to the Hazardous Chemical List: At a minimum, please provide the following information for each new hazardous chemical you have added to the lab / area inventory since the last update or not included on your inventory:

Name of chemical as printed on the container label CAS registry number (can be found on container label or MSDS) NFPA Rating (hazard level for Health, Flammability, and Reactivity), including any

special hazards such as Carcinogen (CA), Oxidizer (OX), Corrosive (COR), Acid (ACID), Poison – highly toxic (TOX), and Water-reactive (W).

Source or manufacturer Size of container or original quantity of chemical. Also include the number of

containers on hand. o Example: 4 x 4L of Methanol

DO NOT include the following types of chemicals in your inventory:

Non-hazardous chemicals and retail consumer goods used for their intended purpose. o Example: Easy Off Fume Free Max Oven Cleaner or Arm & Hammer Baking

Soda. Reagents and Stock Solutions containing less than 1% of a hazardous chemical, or less

than 0.1% of a highly toxic or carcinogenic chemical.

Revision 7/17/2013 2

o Example: 0.5% solution of Tris Buffer would not be included, but a 0.5% solution of benzene would.

Items considered “Articles” under the law, such as ink pens, markers, alcohol pads, and blood collection tubes, which may contain small amounts of hazardous chemicals, but under normal use, there is little or no risk of human exposure. Contact Environmental Health & Safety if you have questions about articles.

Principal Investigator / Supervisor Assurances: I have reviewed the online Chemical Inventory for the lab(s) /area(s) listed above. I have made the necessary changes to my inventory, and by submission of this signed memo, assure that the information on file with Environmental Health & Safety is correct. Changes / additions / deletions noted online Changes / additions / deletions attached to this memo

No Changes / additions / deletions noted

Comments: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ PI/Supervisor Signature: __________________________________ Date: _______________ Signature & Date Required by the Texas Hazard Communication Act; 2 THSC § 502.005(b) (SAFETY OFFICE USE ONLY) PI Name (print): Date: Laboratory Areas: Changes verified by: ____________________________________ Date: ____________________ Comments: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

University of Texas Health Science Center at San Antonio Environmental Health & Safety Department - Chemical Safety Division Page 1 of 4

Laboratory Safety Evaluation Record

Revision 09/1/2013

Building: Choose an item.

Room/Area:

Safety Specialist/Manager:

Date of Evaluation:

PI or Area Supervisor/Manager:

Department:

Contact Person:

Contact Phone Number:

Street Address:

City:

Zip Code:

ROOM TYPE (as listed in EHS Assistant):

RES-Research AUT-Autoclave INA-Inactive lab PET-PET scanner TEA-Teaching lab

TCL-Tissue Culture EP-Energy plant MIC - Microscope PHY-Physical Plant WHS-Warehouse

CLL-Clinical Lab CHA-Utility chase MDL-Multi-disc. lab PRL-Prosthetic lab WIC-Walk-in cold room

ANA-Anatomy Lab DTL-Dental lab MEC-Mechanical PRO-Procedure WII-Walk-in incubator

ANI-Animal holding DRK-Dark Room WMU–Waste

Management Unit RII-Research Imaging

Institute FUM-Fume hood

alcove

ANL-Animal Res. EQU-Equipment

OTH-Other room

List type:

STO-Storage room OLB-Open lab bench

DD-Dental Dispensary CYC-Cyclotron MRI – MRI room ABR-Animal Behavior PHA-Pharmacy

QUA-Quarantine Room CAG-Cage Wash RAD-Rad Waste PRL-Prosthetics lab FLO-Flow Cytometry

COR-Corridor COM-Common Use LAS-Laser Room XRA-X-Ray Room ANS-Animal Surgical Suite

2) BIOLOGICAL AGENTS rDNA? NO YES List type(s): Select Agents? NO YES List type(s): Transgenic animals? NO YES List type(s): Lenti-virus? NO YES List type(s): Bacterial Agents? NO YES List type(s): Other Virus? NO YES List type(s): Fungal Agents? NO YES List type(s): Pathogens? NO YES List type(s): Toxins? NO YES List type(s): If YES, is the lab cloning the toxin with a virus or e. coli? NO YES NO = CSC YES = IBC & CSC OTHER BIOLOGICAL AGENTS IN USE: Biosafety Level of Containment (BSL): Hepatitis B Vaccinations? NO YES N/A Other Medical Surveillance Requirements: IBC APPROVAL on File? NO YES N/A Approval Date: Expiration Date: Requires renewal? NO YES N/A Biosafety Level, Bioagents & Medical Surveillance Requirements on placard? NO YES N/A Comments:

3) CHEMICAL HAZARDS NO YES

Peroxide former Pyrophoric Heavy Metals Chemotherapy/Antineoplastic Nanomaterials DHS Chemicals

List Other Types of Chemical Hazards: CSC APPROVAL: NO YES N/A Approval Date: Expiration Date: Requires renewal? NO YES N/A

University of Texas Health Science Center at San Antonio Environmental Health & Safety Department - Chemical Safety Division Page 2 of 4

Laboratory Safety Evaluation Record

Revision 09/1/2013

HAZARDOUS WASTE SATELLITE COLLECTION SITE? NO YES IF YES, LIST WASTE: Chemical Exposure Monitoring Required for: choose an item Other, please list: Date of Last Monitoring:

4) Radioactive materials used? NO YES

IF YES, Select Isotope(s): 32-P 3-H 35-S 14-C 33-P 125-I List Other Types: RSC APPROVAL: NO YES N/A

Symbol Posted on Placard? NO YES N/A

5) X-Ray Machines in use? NO YES TRAINING: NO YES N/A Symbol Posted on Placard? NO YES N/A

6) Are any LASERS in use? NO YES

Select TYPE: Choose an item. Other: TRAINING: NO YES N/A

Symbol Posted on Placard? NO YES N/A

7) Are special hazards present? NO

YES, Select TYPE: UV - High Voltage - PET - MRI - Mercury-containing devices - Other – list:

8) Does the lab ship Class 6.2 Infectious Materials, Category B, or other hazardous materials? NO YES If Yes, is training on file? NO YES

Name(s) of person(s) trained: Click here to enter text. Is training current (every 2 years if shipping by air)? NO YES

9) PERSONNEL TRAINING LAST NAME FIRST

NAME HSC BADGE #

NEO Safety Awareness

DATE

SITE-SPECIFIC Chemical Hazard

DATE

LABORATORY Safety & Hazardous Waste Generator’s

DATE

BASIC Bloodborne Pathogens

DATE

BASIC Biological Safety

DATE

BASIC Radiation Safety Orient 9/20 Hr DATE DATE

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXX

University of Texas Health Science Center at San Antonio Environmental Health & Safety Department - Chemical Safety Division Page 3 of 4

Laboratory Safety Evaluation Record

Revision 09/1/2013

Any unsatisfactory safety condition or concern must be relayed to the appropriate UT Health Science Center constituent utilizing the Chemical Safety Division - Laboratory Evaluation Deficiency Report. Date Performed: Deficiencies noted: Report Date: SIGNATURES This facility has been evaluated against criteria specified in the UT Health Science Center Chemical, Biological, Radiation, and Physical Standards Safety Handbooks.

Evaluated By: Choose an item. Date evaluated:

Reviewed By: Choose an item. Date reviewed:

Evaluation Notes:

University of Texas Health Science Center at San Antonio Environmental Health & Safety Department - Chemical Safety Division Page 4 of 4

Laboratory Safety Evaluation Record

Revision 09/1/2013

Date Performed: _____________ Lab / Room: __________________

P.I. / Supervisor: _________________ Procedure: Evaluate each of the following laboratory items according to the requirements of the appropriate UTHSCSA

Safety Handbook(s). Place a check in the appropriate space for Y (Yes), N (No), N/O (Not Observed), or N/A (Not Applicable). Enter comments in the space provided.

Y N N/O N/A COMMENTS? GENERAL POSTINGS

1. NFPA 704 Code

2. No Food or Drink

3. Radiation

4. Biohazard (Entry/Exit Procedures Posted?)

5. UV Light

6. LASER

7. Current emergency phone numbers posted?

8. TDH Hazard Communic. Notice to Employees

9. TDH Radiation Notice to Employees GENERAL SAFETY

10. Current Chemical Safety Handbook available?

11. Current Biological Safety Handbook available?

12. Current Radiation Safety Handbook available?

13. Current Physical Safety Standards Handbook available?

14. Current Emergency Evacuation & Response Plan available?

15. Appropriate training records on file?

16. General housekeeping orderly?

17. No food or drink observed in lab? GENERAL IAQ 18. No unusual odors present?

19. Is room pressure negative to corridor?

20. No visible particulates coming from vents? FIRE SAFETY

21. Fire egress unobstructed?

22. Access to pull alarms & fire equipment clear?

23. Fire extinguisher available & inspected?

24. Smoke detector present?

25. Self-closing door to lab functional?

26. Path to electric panel & gas shut off clear?

27. Natural gas lines functional, labeled, & used properly?

28. Heat sources separated from combustibles?

29. 18 inches clearance to sprinklers, lights & air vents?

30. Absence of slip, trip, & cut hazards?

31. Compressed gas cylinders secured?

32. Noise levels acceptable (action level = 85 dB)?

33. Guards in place for mechanical hazards?

34. Electrical equipment grounded properly?

35. Extension cords not in use (except emergency)?

36. Circuits / outlets not overloaded?

University of Texas Health Science Center at San Antonio Environmental Health & Safety Department - Chemical Safety Division Page 5 of 4

Laboratory Safety Evaluation Record

Revision 09/1/2013

Y N N/O N/A COMMENTS? BIOLOGICAL SAFETY

37. Standard / Universal precautions utilized?

38. Potentially exposed workers offered vaccines / medical surveillance?

39. Vaccination records available?

40. PPE available and used as required?

41. Hand washing facilities adequate?

42. Refrigerators, freezers, instrumentation posted with biohazard label – no food or drink label as appropriate?

43. Biological Safety Cabinet certified annually?

44. U V lamps used properly & posted?

45. Medical waste / sharps properly stored and handled?

46. Chairs used in lab are non-porous material? 47. Inline HEPA filters used to protect vacuum lines? 48. IBC approval on file? CHEMICAL SAFETY

49. Hazardous chemical list / inventory updated?

50. CSC approval to work with carcinogens on file?

51. Required (OSHA) chemical monitoring on file?

52. Appropriate warning signs posted (hood, refrigerators, etc.)?

53. Controlled substances secured?

54. Excessive chemicals not stored in fume hood?

55. Linear flow rate in fume hood adequate (80 – 120, avg. 100 lfpm) and tested annually?

56. Chemicals stored and segregated properly?

57. Secondary containers labeled properly (hazards & date received)?

58. No very old or potentially explosive chemicals?

59. No flammables stored in general purpose refrigerator/freezer?

60. Hazardous liquid chemicals stored below employee ‘s eye level or 6 feet?

61. Safety shower / eyewash available within 100 ft. or 10 seconds travel?

62. Employees able to locate MSDS & Safety Handbook(s)?

63. Chemical waste properly stored? Liquid waste capped / closed?

64. Chemical waste properly labeled including approximate component %?

65. Previous deficiencies adequately resolved?

66. There are no additional safety concerns? Any unsatisfactory safety condition or concern must be relayed to the appropriate UT Health Science Center constituent utilizing the Chemical Safety Division - Laboratory Evaluation Deficiency Report.

Date Performed: ___________ Report Date: ___________

SIGNATURES This facility has been evaluated against criteria specified in the UT Health Science Center Chemical, Biological, Radiation, and Physical Standards Safety Handbooks. Evaluated By: _________________________________ Date evaluated: ________________________________

Reviewed By: _________________________________ Date reviewed: ________________________________

Site-Specific Chemical Hazard Training Form

OSHA's Laboratory Standard (29 CFR 1910.1450) requires that each laboratory employee, including students, be made aware of the location and content of the laboratory's Chemical Hygiene Plan. The Chemical Safety Handbook serves as UTHSCSA’s Written Hazard Communication Plan and Chemical Hygiene Plan. The OSHA Laboratory Safety Standard further requires that the employee's supervisor provide training, which covers the specific topics described in the checklist below. This training must be provided at the time of the employee's initial assignment, on a refresher basis and upon updating procedures. A current copy of this signed form must be on file for each laboratory worker with Environmental Health & Safety. Name of Employee__________________________________ Dept/Campus ____________________________________ UTHSCSA Badge # _________________________________ Lab(s) __________________________________________ Job Title __________________________________________ Work Phone______________________________________ PI/Supervisor______________________________________

Description of Training:

__________________________________________________________________________________________

__________________________________________________________________________________________

__________________________________________________________________________________________

Acknowledgement: I acknowledge that I have read and understood the contents of all safety plans for UTHSCSA and know its location within the laboratory. I have received adequate training on the specific hazards present in the laboratory, including any laboratory-specific procedures. All my questions have been answered to my satisfaction and I understand that I should contact my supervisor or Environmental Health & Safety should my duties change which would require additional training. __________________________________________________________________________________________ Employee Signature Date __________________________________________________________________________________________ Signature of Principal Investigator / Supervisor Date The Environmental Health & Safety Office offers a course entitled Laboratory Safety & Hazardous Waste Generator’s Training Course. This mandatory course for all new employees who work with hazardous chemicals/materials covers the following topics:

General Safety Awareness - safety handbooks, departmental procedures, material safety data sheets (MSDS), personal protective equipment, chemical fume hoods, & biological safety cabinets

Hazardous Chemicals: Definition, Recognition & Labeling Requirements – NFPA 704 code, RCRA requirements

Classification of Hazardous Chemicals & Hazardous Wastes - Regulatory agencies (DOT, EPA/RCRA, OSHA, TCEQ, SAWS), physical hazards, health hazards, hazardous waste characteristics

Handling and Storage of Hazardous Chemicals and Hazardous Waste – Safe handling of chemicals in the laboratory, secondary containment; chemical incompatibilities, chemical & regulated medical waste pick-up and disposal procedures

The University of Texas Health Science Center at San Antonio Environmental Health & Safety Department

Additional Training Requirements UTHSCSA Waste Recycling Programs - Dental amalgam-Mercury, used oils, batteries, silver, etc. Emergency Response – chemical spills, injuries, fire

Laboratory-Specific Training Checklist

In addition to the required Laboratory Safety & Hazardous Waste Generator’s Training Course, each Principal Investigator/Laboratory Supervisor should conduct laboratory-specific training. This checklist may be used to assist employers with the laboratory-specific training requirements outlined in the OSHA Laboratory Safety Standard (29 CFR 1910.1450). Many of these topics can be found in the current Chemical Safety Handbook – Revision 4, February 2007.

Safety Handbooks, MSDS (Chapter 2.0), Laboratory-Specific Standard Operating Procedures Review contents of Chemical Safety Handbook Review procedures for how to access MSDS Review laboratory-specific procedures, equipment in use

Chemical Inventory (Chapters 3.0, 4.0, 6.0, 8.0) Review chemicals used and define hazards Safe handling, locations for storage and segregation of hazardous chemicals, container labeling

Compressed Gas Cylinders / Liquid Nitrogen (Chapter 5.0) Inspection, storage, labeling, and handling of cylinders

Emergency Information Review location of fire extinguishers, fire alarm pull stations, safety showers, eyewash stations,

first aid supplies Review Laboratory-Specific Evacuation Plan – alarm activation, building evacuation & re-entry

procedures, equipment shutdown procedures, and special consideration for non-fire hazards (poisons, corrosives, irritants, radioactive materials, biological hazards)

Chemical Waste Management Procedures (Chapter 12.0) Selection of Containers, Labeling, Secondary Containment Laboratory’s Storage Location for Hazardous Waste How to Request a Chemical Pick-Up

Chemical Purchasing/Procurement Review UTHSCSA Procurement Card Policy Laboratory’s Procedures for Ordering Chemicals

Annual Laboratory Safety Evaluations (Chapter 9.0) Review Results of Most Recent Lab Safety Evaluations and Corrective Actions

Laboratory Hygiene & Personal Protective Equipment (Chapter 10.0) Personal Protective Equipment (PPE) Requirements for the Lab Laboratory-Specific Housekeeping Procedures

Environmental Monitoring (Chapter 7.0) & Safety Equipment Review Use of Specific Chemicals That Would Warrant Chemical Exposure Monitoring How to Request Monitoring from Environmental Health & Safety Review Laboratory Directional Airflow, Chemical Fume Hoods, Biological Safety Cabinets

Medical Surveillance Review Laboratory-Specific Requirements - Hepatitis B Virus Vaccine, TB Skin Test, Baseline

Serum Sampling, Respirator Use & Fit Test Chemical Spills and Emergency Response (Chapter 13.0)

Review Laboratory-Specific Spill Response, Decontamination, Procedure for Reporting Injuries & Illnesses

Review Recent Spills/Accidents/Injuries and Discuss Prevention Measures Post Phone Number to South Texas Poison Center (1-800-222-1222)

Send signed copy to Environmental Health & Safety Office, 1.343T DTL

RETAIN COPY ON FILE FOR 5 YEARS Revision 11/26/2007 Reviewed 10/24/2012

Environmental Health & Safety Revised December 2011

Environmental Health & Safety Department Laboratory Decommissioning Risk Assessment

Principal Investigator: _______________________________ Department: ___________________________

Lab(s): ___________________________________________ Building: ______________________________

Hazardous Materials Responsible Laboratory Personnel Reviewed by EH&S Section 1. Chemicals Evaluate all chemicals for transfer or ________________________________________________________________________ waste disposal Transfer surplus chemicals to other ________________________________________________________________________ UTHSCSA Principal Investigators Dispose of waste chemicals through ________________________________________________________________________ Environmental Health and Safety (all chemicals must have disposal tags affixed to each container) Contact EH&S for destruction of Select ________________________________________________________________________ Agent Toxins Contact UTPD for destruction of DEA ________________________________________________________________________ controlled substances Return gas cylinders to supplier ________________________________________________________________________ (non-returnable cylinders may be disposed of through EH&S) Section 2. Radioactive Materials Inform the Radiation Safety Officer of ________________________________________________________________________ your intended move Transfer surplus radioactive stocks or ________________________________________________________________________ sealed sources to another authorized Permit within HSC Dispose of all waste radioactive material ________________________________________________________________________ through EH&S Decontaminate and wipe test all areas of ________________________________________________________________________ laboratory (below 1000 dpm/100 cm2)

Environmental Health & Safety Revised December 2011

Hazardous Materials Responsible Laboratory Personnel Reviewed by EH&S

Section 3. Biological Materials Inform the Biological Safety Manager ________________________________________________________________________ of your intended move Request decontamination of biosafety ________________________________________________________________________ cabinets through EH&S Remove biohazardous materials ________________________________________________________________________ (cultures, frozen stocks, tissues, etc.) by proper transferring to another investigator, autoclaving or incineration as appropriate. Contact EHS for assistance. Clean and disinfect all laboratory surfaces ________________________________________________________________________ and equipment Section 4. Housekeeping Clean and decontaminate laboratory _________________________________________________________________________ equipment (including chemical fume hoods) Clean and decontaminate all laboratory _________________________________________________________________________ surfaces Ensure that all garbage, laboratory supplies, _________________________________________________________________________ glassware, books, binders or debris are removed from the laboratory. Submit Equipment Clearance request for _________________________________________________________________________ all items to be removed from laboratory Section 5. X-Ray Machines, Lasers Contact Radiation Safety Officer ________________________________________________________________________ Remove item from inventory/registration ________________________________________________________________________ Intellectual Property _____________________________________________________________ Capital Assets ____________________________________________________________

Environmental Health & Safety Revised December 2011

Final Laboratory Walk-Through and Clearance Laboratories are decommissioned after sections below are signed by Principal Investigator, Department Chair, and Environmental Health & Safety representatives. Any outstanding issues must be resolved prior to final decommissioning. A new researcher may not take possession of the laboratory until approved by all parties listed below.

The following were present during walk-through:

Name Department Signature

Date of Walk-Through: _________________

Formal Walk-Through Observations / Notes:

__________________________________________________________________________________________

__________________________________________________________________________________________

__________________________________________________________________________________________

__________________________________________________________________________________________

__________________________________________________________________________________________

__________________________________________________________________________________________

__________________________________________________________________________________________

_________________________________________________________________________________________

Environmental Health & Safety Revised December 2011

Principal Investigator Certification: I acknowledge that all requirements pertaining to the decommissioning and clearance of all UTHSCSA laboratory space assigned have been completed. Name (Print): _____________________________________

Signature: ________________________________________

Date: ____________________________________________

Approved by: Department Chair Name (print): _________________________________

Signature: ____________________________________

Date: ________________________________________

Environmental Health & Safety Chemical Safety Name (print): ______________________________________________

Signature: ________________________________________________

Date: _____________________________________________________

Biological Safety Name (print): ______________________________________________

Signature: ________________________________________________

Date: _____________________________________________________

Radiation Safety Name (print): ______________________________________________

Signature: ________________________________________________

Date: _____________________________________________________

Questions should be directed to Environmental Health & Safety, (210) 567-2955

For Office Use Only

• Notification to Space Management Y N Date:

____________________

• Notification to Grants Management Y N Date:

____________________

• Notification to UT Police Y N Date: