Chapter 8, objective # 17

List the antigens that are denatured by routine blood bank enzymes and antigens whose reactivity with antibody is

enhanced with enzymes?

Antigens denatured activity by enzymes

M and N antigens Easily destroyed by the routine blood bank enzymes ficin, papain, and bromelin.

Less common enzymes are trypsin and pronase.

S and s antigen Less easily degraded by Ficin, papain, bromelin, pronase, and chymotrypsin can destroy S and s activity, but the amount of degradation may depend on the strength of the enzyme solution.

Fya and Fyb antigen Antigens are destroyed by proteolytic enzymes such as ficin, papain, bromelin, and chymotrypsin, and by ZZAP.

Neuramidase may reduce the molecular weight of Fya and Fyb antigen, but it does not destroy antigenic activity and neither does purified trypsin.

Antigens enhanced activity by enzymes

I and i antigen Treatment of RBCs with ficin and papain enhances reactivity of I and I antigens with their respective antibodies.The I and i antigens are resistant to treatment with DTT and glycine- acid EDTA.

P1 It is resistant to different enzymes treatment.Reactivity of the antibodies can be greatly enhanced by testing with enzyme-treated RBCs.

Jk a and Jk b antigen Kidd antigens are not denatured by papain or ficin; treatment of RBCs with enzymes generally enhances reactivity with Kidd antibodies.