Chapter 5 Introduction The Working CellChapter 5 The Working Cell Introduction Some organisms use...

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© 2012 Pearson Education, Inc. Lecture by Edward J. Zalisko

PowerPoint Lectures for

Campbell Biology: Concepts & Connections, Seventh EditionReece, Taylor, Simon, and Dickey

Chapter 5 The Working CellIntroduction

Some organisms use energy-converting reactions to produce light in a process called bioluminescence.

– Many marine invertebrates and fishes use bioluminescence to hide themselves from predators.

– Scientists estimate that 90% of deep-sea marine life produces bioluminescence.

The light is produced from chemical reactions that convert chemical energy into visible light.

© 2012 Pearson Education, Inc.

Figure 5.0_1

Chapter 5: Big Ideas

Membrane Structureand Function

Energy and the Cell

How Enzymes Function

Cellular respiration

Figure 5.0_2

Introduction

Bioluminescence is an example of the multitude of energy conversions that a cell can perform.

Many of a cell’s reactions

– take place in organelles and

– use enzymes embedded in the membranes of these organelles.

This chapter addresses how working cells use membranes, energy, and enzymes.


MEMBRANE STRUCTURE AND FUNCTION


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5.1 Membranes are fluid mosaics of lipids and proteins with many functions

Membranes are composed of

– a bilayer of phospholipids with

– embedded and attached proteins,

– in a structure biologists call a fluid mosaic.



Many phospholipids are made from unsaturated fatty acids that have kinks in their tails.

These kinks prevent phospholipids from packing tightly together, keeping them in liquid form.

In animal cell membranes, cholesterol helps

– stabilize membranes at warmer temperatures and

– keep the membrane fluid at lower temperatures.


Figure 5.1

Fibers ofextracellularmatrix (ECM)

Enzymatic activity

Phospholipid

Cholesterol

CYTOPLASM

CYTOPLASM

Cell-cellrecognition

Glycoprotein

Intercellularjunctions

Microfilamentsof cytoskeleton

ATPTransport

Signaltransduction

Receptor

Signalingmolecule

Attachment to the cytoskeletonand extracellular matrix (ECM)


Membrane proteins perform many functions.

1. Some proteins help maintain cell shape and coordinate changes inside and outside the cell through their attachment to the cytoskeleton and extracellular matrix.

2. Some proteins function as receptors for chemical messengers from other cells.

3. Some membrane proteins function as enzymes.


Animation: Overview of Cell Signaling

Animation: Signal Transduction Pathways


4. Some membrane glycoproteins are involved in cell-cell recognition.

5. Membrane proteins may participate in the intercellular junctions that attach adjacent cells to each other.

6. Membranes may exhibit selective permeability, allowing some substances to cross more easily than others.


5.2 EVOLUTION CONNECTION: Membranes form spontaneously, a critical step in the origin of life

Phospholipids, the key ingredient of biological membranes, spontaneously self-assemble into simple membranes.

The formation of membrane-enclosed collections of molecules was a critical step in the evolution of the first cells.


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Figure 5.2

Water-filledbubble made ofphospholipids

Figure 5.2Q

Water

Water

5.3 Passive transport is diffusion across a membrane with no energy investment

Diffusion is the tendency of particles to spread out evenly in an available space.

– Particles move from an area of more concentrated particles to an area where they are less concentrated.

– This means that particles diffuse down their concentration gradient.

– Eventually, the particles reach equilibrium where the concentration of particles is the same throughout.


5.3 Passive transport is diffusion across a membrane with no energy investment

Diffusion across a cell membrane does not require energy, so it is called passive transport.

The concentration gradient itself represents potential energy for diffusion.


Animation: Membrane Selectivity

Animation: Diffusion

Figure 5.3A

Molecules of dye Membrane

Pores

Net diffusion Net diffusion Equilibrium

Figure 5.3B

Net diffusion Net diffusion

Net diffusion Net diffusion

Equilibrium

Equilibrium

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5.4 Osmosis is the diffusion of water across a membrane

One of the most important substances that crosses membranes is water.

The diffusion of water across a selectively permeable membrane is called osmosis.


Animation: Osmosis

5.4 Osmosis is the diffusion of water across a membrane

If a membrane permeable to water but not a solute separates two solutions with different concentrations of solute,

– water will cross the membrane,

– moving down its own concentration gradient,

– until the solute concentration on both sides is equal.


Figure 5.4

Osmosis

Solute moleculewith cluster ofwater molecules

Watermolecule

Selectivelypermeablemembrane

Solutemolecule

H2O

Lowerconcentration

of solute

Higherconcentration

of solute

Equalconcentrations

of solute

5.5 Water balance between cells and their surroundings is crucial to organisms

Tonicity is a term that describes the ability of a solution to cause a cell to gain or lose water.

Tonicity mostly depends on the concentration of a solute on both sides of the membrane.



How will animal cells be affected when placed into solutions of various tonicities? When an animal cell is placed into

– an isotonic solution, the concentration of solute is the same on both sides of a membrane, and the cell volume will not change,

– a hypotonic solution, the solute concentration is lower outside the cell, water molecules move into the cell, and the cell will expand and may burst, or

– a hypertonic solution, the solute concentration is higher outside the cell, water molecules move out of the cell, and the cell will shrink.



For an animal cell to survive in a hypotonic or hypertonic environment, it must engage in osmoregulation, the control of water balance.


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The cell walls of plant cells, prokaryotes, and fungi make water balance issues somewhat different.

– The cell wall of a plant cell exerts pressure that prevents the cell from taking in too much water and bursting when placed in a hypotonic environment.

– But in a hypertonic environment, plant and animal cells both shrivel.


Video: Paramecium Vacuole

Video: Chlamydomonas

Video: Turgid Elodea

Video: Plasmolysis

Figure 5.5

Animalcell

Plantcell

Turgid(normal)

Flaccid Shriveled(plasmolyzed)

Plasmamembrane

Lysed Normal Shriveled

Hypotonic solution Isotonic solution Hypertonic solution

H2O H2O H2O H2O

H2O H2O H2O

5.6 Transport proteins can facilitate diffusion across membranes

Hydrophobic substances easily diffuse across a cell membrane.

However, polar or charged substances do not easily cross cell membranes and, instead, move across membranes with the help of specific transport proteins in a process called facilitated diffusion, which

– does not require energy and

– relies on the concentration gradient.



Some proteins function by becoming a hydrophilic tunnel for passage of ions or other molecules.

Other proteins bind their passenger, change shape, and release their passenger on the other side.

In both of these situations, the protein is specific for the substrate, which can be sugars, amino acids, ions, and even water.



Because water is polar, its diffusion through a membrane’s hydrophobic interior is relatively slow.

The very rapid diffusion of water into and out of certain cells is made possible by a protein channel called an aquaporin.


Figure 5.6

Solutemolecule

Transportprotein

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5.7 SCIENTIFIC DISCOVERY: Research on another membrane protein led to the discovery of aquaporins

Dr. Peter Agre received the 2003 Nobel Prize in chemistry for his discovery of aquaporins.

His research on the Rh protein used in blood typing led to this discovery.


Figure 5.7

5.8 Cells expend energy in the active transport of a solute

In active transport, a cell

– must expend energy to

– move a solute against its concentration gradient.

The following figures show the four main stages of active transport.


Animation: Active Transport

Figure 5.8_s1

Transportprotein

Solute

Solute binding1

Figure 5.8_s2

Transportprotein

Solute ADPATP P

Solute binding Phosphateattaching

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Figure 5.8_s3

Transportprotein

Solute ADPATP P PProtein

changes shape.


Transport21 3

7

Figure 5.8_s4

Transportprotein

Solute ADPATP P P PProtein

changes shape.Phosphatedetaches.


Transport Proteinreversion

4321

5.9 Exocytosis and endocytosis transport large molecules across membranes

A cell uses two mechanisms to move large molecules across membranes.

– Exocytosis is used to export bulky molecules, such as proteins or polysaccharides.

– Endocytosis is used to import substances useful to the livelihood of the cell.

In both cases, material to be transported is packaged within a vesicle that fuses with the membrane.


5.9 Exocytosis and endocytosis transport large molecules across membranes

There are three kinds of endocytosis.

1. Phagocytosis is the engulfment of a particle by wrapping cell membrane around it, forming a vacuole.

2. Pinocytosis is the same thing except that fluids are taken into small vesicles.

3. Receptor-mediated endocytosis uses receptors in a receptor-coated pit to interact with a specific protein, initiating the formation of a vesicle.


Animation: Phagocytosis

Animation: Exocytosis

Animation: Receptor-Mediated Endocytosis

Animation: Pinocytosis

Animation: Exocytosis and Endocytosis Introduction

Figure 5.9Phagocytosis

Pinocytosis

Receptor-mediated endocytosis

EXTRACELLULARFLUID

CYTOPLASM

Pseudopodium

“Food” orother particle

Foodvacuole

Foodbeingingested

Plasma membrane

Plasma membrane

Vesicle

Receptor

Specificmolecule

Coatedpit

Coatedvesicle

Coat protein

Coatedpit

Material boundto receptor proteins

Figure 5.9_1

Phagocytosis

EXTRACELLULARFLUID

CYTOPLASM

Pseudopodium

“Food” orother particle

Foodvacuole

Foodbeingingested

Figure 5.9_2

Pinocytosis

Plasma membrane

Plasma membrane

Vesicle

8

Figure 5.9_3

Receptor-mediated endocytosis Plasma membrane

Receptor

Specificmolecule

Coatedpit

Coatedvesicle

Coat protein

Coatedpit


Figure 5.9_4

Foodbeingingested

Figure 5.9_5

Plasma membrane

Figure 5.9_6

Plasma membrane

Coatedpit


ENERGY AND THE CELL


5.10 Cells transform energy as they perform work

Cells are small units, a chemical factory, housing thousands of chemical reactions.

Cells use these chemical reactions for

– cell maintenance,

– manufacture of cellular parts, and

– cell replication.


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Energy is the capacity to cause change or to perform work.

There are two kinds of energy.

1. Kinetic energy is the energy of motion.

2. Potential energy is energy that matter possesses as a result of its location or structure.


Figure 5.10

Fuel Energy conversion Waste products

Gasoline

Oxygen

Oxygen

Glucose

Heatenergy

CombustionKinetic energyof movement

Energy conversion in a car

Energy conversion in a cell

Energy for cellular work


ATP ATP

Heatenergy

Carbon dioxide

Carbon dioxide

Water

Water

Figure 5.10_1


Gasoline

Oxygen

Heatenergy

CombustionKinetic energyof movement

Energy conversion in a car

Carbon dioxide

Water

Figure 5.10_2

Oxygen

Glucose

Energy conversion in a cell

Energy for cellular work


ATP ATP

Heatenergy

Carbon dioxide

Water



Heat, or thermal energy, is a type of kinetic energy associated with the random movement of atoms or molecules.

Light is also a type of kinetic energy, and can be harnessed to power photosynthesis.



Chemical energy is the potential energy available for release in a chemical reaction. It is the most important type of energy for living organisms to power the work of the cell.


Animation: Energy Concepts

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Thermodynamics is the study of energy transformations that occur in a collection of matter.

Scientists use the word

– system for the matter under study and

– surroundings for the rest of the universe.



Two laws govern energy transformations in organisms. According to the

– first law of thermodynamics, energy in the universe is constant, and

– second law of thermodynamics, energy conversions increase the disorder of the universe.

Entropy is the measure of disorder, or randomness.



Cells use oxygen in reactions that release energy from fuel molecules.

In cellular respiration, the chemical energy stored in organic molecules is converted to a form that the cell can use to perform work.


5.11 Chemical reactions either release or store energy

Chemical reactions either

– release energy (exergonic reactions) or

– require an input of energy and store energy (endergonic reactions).



Exergonic reactions release energy.

– These reactions release the energy in covalent bonds of the reactants.

– Burning wood releases the energy in glucose as heat and light.

– Cellular respiration

– involves many steps,

– releases energy slowly, and

– uses some of the released energy to produce ATP.


Figure 5.11A

Reactants

EnergyProducts

Amount ofenergy

released

Po

ten

tial

en

erg

y o

f m

ole

cule

s

11


An endergonic reaction

– requires an input of energy and

– yields products rich in potential energy.

Endergonic reactions

– begin with reactant molecules that contain relatively little potential energy but

– end with products that contain more chemical energy.


Figure 5.11B

Reactants

Energy

Products

Amount ofenergy

required

Po

ten

tial

en

erg

y o

f m

ole

cule

s


Photosynthesis is a type of endergonic process.

– Energy-poor reactants, carbon dioxide, and water are used.

– Energy is absorbed from sunlight.

– Energy-rich sugar molecules are produced.



A living organism carries out thousands of endergonic and exergonic chemical reactions.

The total of an organism’s chemical reactions is called metabolism.

A metabolic pathway is a series of chemical reactions that either

– builds a complex molecule or

– breaks down a complex molecule into simpler compounds.



Energy coupling uses the

– energy released from exergonic reactions to drive

– essential endergonic reactions,

– usually using the energy stored in ATP molecules.


ATP, adenosine triphosphate, powers nearly all forms of cellular work.

ATP consists of

– the nitrogenous base adenine,

– the five-carbon sugar ribose, and

– three phosphate groups.

5.12 ATP drives cellular work by coupling exergonic and endergonic reactions


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Hydrolysis of ATP releases energy by transferring its third phosphate from ATP to some other molecule in a process called phosphorylation.

Most cellular work depends on ATP energizing molecules by phosphorylating them.


Figure 5.12A_s1

AdenineP P P

Phosphategroup

ATP: Adenosine Triphosphate

Ribose

Figure 5.12A_s2

ADP: Adenosine Diphosphate

P P P Energy

H2OHydrolysis

Ribose

AdenineP P P

Phosphategroup

ATP: Adenosine Triphosphate 5.12 ATP drives cellular work by coupling exergonic and endergonic reactions

There are three main types of cellular work:

1. chemical,

2. mechanical, and

3. transport.

ATP drives all three of these types of work.


Figure 5.12B

ATP ATP ATP

ADP ADP ADPP P P

P

P

P

PP

P

Chemical work Mechanical work Transport work

Reactants

Motorprotein

Solute

Membrane protein

Product

Molecule formed Protein filament moved Solute transported


ATP is a renewable source of energy for the cell.

In the ATP cycle, energy released in an exergonic reaction, such as the breakdown of glucose,is used in an endergonic reaction to generate ATP.


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Figure 5.12C

Energy fromexergonicreactions

Energy forendergonicreactions

ATP

ADP P

HOW ENZYMES FUNCTION


5.13 Enzymes speed up the cell’s chemical reactions by lowering energy barriers

Although biological molecules possess much potential energy, it is not released spontaneously.

– An energy barrier must be overcome before a chemical reaction can begin.

– This energy is called the activation energy (EA).



We can think of EA

– as the amount of energy needed for a reactant molecule to move “uphill” to a higher energy but an unstable state

– so that the “downhill” part of the reaction can begin.

One way to speed up a reaction is to add heat,

– which agitates atoms so that bonds break more easily and reactions can proceed but

– could kill a cell.


Figure 5.13A

Activationenergy barrier

Reactant

Products

Without enzyme With enzyme

Reactant

Products

Enzyme

Activationenergy barrierreduced byenzyme

En

erg

y

En

erg

y

Figure 5.13A_1

Activationenergy barrier

Reactant

Products

Without enzyme

En

erg

y

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Figure 5.13A_2

Activationenergy barrierreduced byenzyme

Reactant

Products

With enzyme

En

erg

y

Enzyme

Figure 5.13Q

Reactants

Products

En

erg

y

Progress of the reaction

a

b

c


Enzymes

– function as biological catalysts by lowering the EA

needed for a reaction to begin,

– increase the rate of a reaction without being consumed by the reaction, and

– are usually proteins, although some RNA molecules can function as enzymes.


Animation: How Enzymes Work

5.14 A specific enzyme catalyzes each cellular reaction

An enzyme

– is very selective in the reaction it catalyzes and

– has a shape that determines the enzyme’s specificity.

The specific reactant that an enzyme acts on is called the enzyme’s substrate.

A substrate fits into a region of the enzyme called the active site.

Enzymes are specific because their active site fits only specific substrate molecules.



The following figure illustrates the catalytic cycle of an enzyme.


Figure 5.14_s1

1

Enzyme(sucrase)

Active site

Enzyme availablewith empty activesite

15

Figure 5.14_s2

2

1

Enzyme(sucrase)

Active site


Substrate(sucrose)

Substrate bindsto enzyme withinduced fit

Figure 5.14_s3

3

2

1

Enzyme(sucrase)

Active site


Substrate(sucrose)


Substrate isconverted toproducts

H2O

Figure 5.14_s4

4

3

2

1

Products arereleased

Fructose

Glucose

Enzyme(sucrase)

Active site


Substrate(sucrose)


Substrate isconverted toproducts

H2O


For every enzyme, there are optimal conditions under which it is most effective.

Temperature affects molecular motion.

– An enzyme’s optimal temperature produces the highest rate of contact between the reactants and the enzyme’s active site.

– Most human enzymes work best at 35–40ºC.

The optimal pH for most enzymes is near neutrality.



Many enzymes require nonprotein helpers called cofactors, which

– bind to the active site and

– function in catalysis.

Some cofactors are inorganic, such as zinc, iron, or copper.

If a cofactor is an organic molecule, such as most vitamins, it is called a coenzyme.


5.15 Enzyme inhibitors can regulate enzyme activity in a cell

A chemical that interferes with an enzyme’s activity is called an inhibitor.

Competitive inhibitors

– block substrates from entering the active site and

– reduce an enzyme’s productivity.


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Noncompetitive inhibitors

– bind to the enzyme somewhere other than the active site,

– change the shape of the active site, and

– prevent the substrate from binding.


Figure 5.15A

Substrate

Enzyme

Allosteric site

Active site

Normal binding of substrate

Competitiveinhibitor

Noncompetitiveinhibitor

Enzyme inhibition


Enzyme inhibitors are important in regulating cell metabolism.

In some reactions, the product may act as an inhibitor of one of the enzymes in the pathway that produced it. This is called feedback inhibition.


Figure 5.15B

Feedback inhibition

Startingmolecule

Product

Enzyme 1 Enzyme 2 Enzyme 3

Reaction 1 Reaction 2 Reaction 3A B C D

5.16 CONNECTION: Many drugs, pesticides, and poisons are enzyme inhibitors

Many beneficial drugs act as enzyme inhibitors, including

– Ibuprofen, inhibiting the production of prostaglandins,

– some blood pressure medicines,

– some antidepressants,

– many antibiotics, and

– protease inhibitors used to fight HIV.

Enzyme inhibitors have also been developed as pesticides and deadly poisons for chemical warfare.


Figure 5.16

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1. Describe the fluid mosaic structure of cell membranes.

2. Describe the diverse functions of membrane proteins.

3. Relate the structure of phospholipid molecules to the structure and properties of cell membranes.

4. Define diffusion and describe the process of passive transport.

You should now be able to


5. Explain how osmosis can be defined as the diffusion of water across a membrane.

6. Distinguish between hypertonic, hypotonic, and isotonic solutions.

7. Explain how transport proteins facilitate diffusion.

8. Distinguish between exocytosis, endocytosis, phagocytosis, pinocytosis, and receptor-mediated endocytosis.



9. Define and compare kinetic energy, potential energy, chemical energy, and heat.

10. Define the two laws of thermodynamics and explain how they relate to biological systems.

11. Define and compare endergonic and exergonic reactions.

12. Explain how cells use cellular respiration and energy coupling to survive.




13. Explain how ATP functions as an energy shuttle.

14. Explain how enzymes speed up chemical reactions.

15. Explain how competitive and noncompetitive inhibitors alter an enzyme’s activity.

16. Explain how certain drugs, pesticides, and poisons can affect enzymes.


Figure 5.UN01

Lower solute concentrationLower free water

concentrationLower soluteconcentration

HIgher free waterconcentration

HIgher soluteconcentration

HIgher solute concentration

Solute

WaterATP

Facilitateddiffusion

Passive transport(requires no energy)

Active transport(requires energy)

Diffusion Osmosis

Figure 5.UN02

ATP cycle ATP

ADP P

Energy fromexergonicreactions

Energy forendergonicreactions

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Figure 5.UN03

Molecules crosscell membranes

passivetransport

polar moleculesand ions

diffusion

movingdown

movingagainst

requires

by by

may be

uses

uses

of of

ATP

(a)

(b)

(c)

(d)(e)

Figure 5.UN04

a.

b.

c.

d.

e.

f.

Table 5.UN05 Figure 5.UN06

pH

Rat

e o

f re

acti

on

109876543210

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