Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

33
Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

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Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation. Mikroskooppi. Numeerinen apertuuri NA=n sin q n=refraktioindeksi. Valomikroskooppi. Laktobasillit gramvärjäys. Gramvärjäys. Darkfield mikroskooppi. Darkfield kuva, verinäyte. Valomikroskooppin rajat. - PowerPoint PPT Presentation

Transcript of Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Page 1: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Chapter 2: The Study of Microbial Structure: Microscopy

and Specimen Preparation

Page 2: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation
Page 3: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Mikroskooppi

Numeerinen apertuuriNA=n sinn=refraktioindeksi

Page 4: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation
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Valomikroskooppi

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Laktobasillit gramvärjäys

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Gramvärjäys

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Darkfield mikroskooppi

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Darkfield kuva, verinäyte

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Valomikroskooppin rajat

• Resolutio maks. 200 nm

• Suurenus maks. noin 1000x

Clostridium botulinum (1000x) 2000x

Page 11: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Faasikontrasti mikroskooppi

Page 12: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Faasikontrasti mikroskooppi

Page 13: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Faasikontrasti ’Tavallinen’

Page 14: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Pseudomonas fluorescence faasikontrasti

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Fluoresenssi mikroskooppi

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emissio & eksitaatio

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Page 18: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation
Page 19: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Green fluorescent protein

Page 20: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Läpäisy-elektroni mikroskooppi

Page 21: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation
Page 22: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

määrätä proteiinit

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’Verjäys’ + määrätä lipidit

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näyttä ’muovissa’

Page 25: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Timanttiveitsi

Page 26: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Kuppariristikko

Page 27: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Läpäisy-elektroni mikroskooppi

Page 28: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Läpäisy-elektroni mikroskooppi

Page 29: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Pyyhkäiselektroni mikroskooppi

Page 30: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Pyyhkäiselektroni mikroskooppi

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Konfokali mikroskooppi

Page 32: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Konfokali mikroskooppiS

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Page 33: Chapter 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

AVAINSANAT

• Valomikroskooppi

• Darkfield mikroskooppi

• Faasikontrasti mikroskooppi

• Fluoresenssi mikroskooppi

• Transmissio elektroni mikroskooppi

• Skanning elektroni mikroskooppi

• Konfokali mikroskooppi


Principles of Microscopy notes-2 - BioDIP€¦ · Microscopy Microscopes must provide: • Resolution ability to carry information about fine detail in the specimen to the image •

Principles of Microscopy notes-2 - BioDIP€¦ · Microscopy Microscopes must provide: • Resolution ability to carry information about fine detail in the specimen to the image •

Ge 116 Module 1: Scanning Electron Microscopy Part 1: Electron optics, beam- specimen interactions & imaging.

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Atomic Force Microscopy of Microbial Cells · Atomic Force Microscopy of Microbial Cells Author: Bruker Corporation Subject: Atomic Force Microscopy of Microbial Cells Keywords: Microbial

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Lecture-4 I Scanning Electron Microscopy What is SEM & Working principles of SEM Major components and their functions Electron beam - specimen interactions.

Lecture-4 I Scanning Electron Microscopy What is SEM & Working principles of SEM Major components and their functions Electron beam - specimen interactions.

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Extending X-Ray Imaging of Noncrystalline …...X-ray diffraction microscopy, oversampling method, lensless imaging, phase retrieval, noncrystalline specimen, single-molecule imaging,

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improved procedure for immunoelectron microscopy ... · with osmiumtetroxide and ultrathin plastic embedding. With respect to staining ofthe specimen, treatment with 0.5%osmiumtetroxide

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Microscopy fits needs of researcher, type of specimen being viewed Brightfield Brightfield Darkfield Darkfield DIC/Nomarski DIC/Nomarski Ultrastructural.

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Preview of “ZEISS Microscopy Online st in Transmitted Light” › microscopy › files › 2018 › 06 › ... · shown in Figure 1(e) using an Obelia hydroid specimen. Finally,

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The use of microscopy and three-dimensional visualization ... · Harrison et al.- The use of microscopy and three-dimensional visualization to evaluate the structure of microbial

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Optical microscopy Optics Lenses and image formation Depth of field Numerical aperture Resolution Instrument Specimen preparation Contrast Examples Optics.

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