2013 DOI ?-Amylase an Enzyme Specificity Found in Various Families of Glycoside Hydrolases
Chapter 15 Enzyme Specificity and Regulation. 15.1 Specificity Is the Result of Molecular...
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Transcript of Chapter 15 Enzyme Specificity and Regulation. 15.1 Specificity Is the Result of Molecular...
Chapter 15 Enzyme Specificity and Regulation
15.1 Specificity Is the Result of Molecular Recognition
15.1.1 Enzyme Specificity
• Structural specificity• Stereospecificity
15.1.2 Hypothesis of Enzyme Action
• the “lock and key” hypothesis
• the “induced fit” hypothesis
15.1.3 Active Site of Enzyme
The specific site on the enzyme where substrate binds and catalysis occurs is called the active site.
15.2 Allosteric Regulation
Feedback inhibition (or regulation)
A B C D ZE1 E2 E3 En
A B C
D Y
E Z E1 E2
E3 E4 En
E3*E4* En*
Sigmoidal curve
Allosteric activator Allosteric inhibitorCO2 + Glu + ATP
CTP
N-carbamoylaspartate
Asp ATCase
Carbamoyl phosphateActivation
Inhibition
15.3 Reversible covalent modification
15.4 Proteolytic Activation
15.4.1 Pancreatic Proteases
Trypsinogen
Enteropeptidase
Trypsin
Chymotrypsinogen Proelastase
Chymotrypsin Elastase
15.4.2 Blood Clotting Cascade
15.5 Regulation of Enzyme Synthesis and breakdown15.6 Isozyme