Differential Gene Expression Dennis Kostka, Christine Steinhoff
Chapter 13 Differential Gene Expression & plant development.
-
Upload
gary-stokes -
Category
Documents
-
view
238 -
download
3
Transcript of Chapter 13 Differential Gene Expression & plant development.
microRNAs and RNAi RNAi – RNA interference
– Used to determine the consequences of down-regulating gene expression
Technologies used in gene identification
RNAi Mutational studies Transposon tagging Microarray analysis
Transposon tagging utilizes colorimetric expression assays
GUS reporter gene (B-glucuronidase), E. coli
GFP (green fluorescent protein), jellyfish
Microarrays and development
Example– Vegetative meristem
mRNA labeled green– Flower bud mRNA
labeled red
The discovery of microRNAs has resulted in which gene
analysis technology?
1. RNAi2. Chemical mutagenesis3. Transposon tagging4. Microarray analysis
GUS and GFP reporter genes are utilized in
1. RNAi2. Chemical mutagenesis3. Transposon tagging4. Microarray analysis
Comparing gene expression under two different conditions is best
accomplished using
1. RNAi2. Chemical mutagenesis3. Transposon tagging4. Microarray analysis