Chap4.1 Eukaryotic Cell

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    CHAPTER 4

    EUKARYOTIC CELL

    STRUCTURE & FUNCTION

    External Structures

    Internal Structures

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    Overview of Eukaryotic CellStructure

    Eukaryote: Protozoa, Algae, Fungi larger than prokaryotic cell

    more complex than prokaryotic cell

    contains membrane-bound organellesthat perform specific functions

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    Protozoa, Algae, Fungi

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    Eukaryotic Cell Structure

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    1. Cilia motility

    5-20 m long

    shorter and more numerous

    than flagella

    beat with two phases

    2. Flagella

    motility

    100-200 m long

    longer than cilia

    eukaryotic flagellum moveslike a whip

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    7/29Ciliated protozoa Oxytrichasp.

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    Structure of Flagella and Cilia membrane-bound cylinders ~2m in diameter

    9 pairs of microtubule doublets circle around 2 central tubules

    9 + 2 arrangement called axoneme

    basal body

    at base of flagellum or cilium directs synthesis of flagella and cilia

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    3. Pellicle a thin layer supporting plasma membrane in various protozoa

    not as strong or rigid as cell wall

    provides characteristic shape to cell

    Epidinium caudatum Pellicle of a mushroom

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    4. Cell Wall

    rigid covering outside plasma membrane

    variable compositions in eukaryotes

    Algae - ?

    Diatoms - ? Fungi - ?

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    Cellulose cell wall of algae Volvoxsp.

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    Silica shell of diatoms

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    Chitin & cellulose of fungi

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    5. Plasma Membrane almost as similar as prokaryotes

    Fluid-mosaic structure

    greater variety oflipids, includephosphoglycerides, sphingolipids andcholesterol

    contains sterols that adds rigidity toplasma membrane

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    (1) is standard lipid bilayer(2) is a lipid raft.

    Plasma Membrane

    eucaryotic membranes contain

    microdomains called lipid rafts

    enriched for certain lipids and proteins

    participate in a variety of cell processessuch as cell movement and transduction

    http://upload.wikimedia.org/wikipedia/commons/c/c6/Lipid_raft_organisation_scheme.svg
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    Eukaryotic Cell InternalStructures

    Nucleus

    EndoplasmicReticulum

    Golgi Apparatus

    Mitochondria

    Chloroplast

    Ribosome

    Lysosome Peroxisome

    Vacuoles

    Cytoplasmicmatrix

    Cytoskeleton

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    Eukaryotic Cell Structure

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    Nucleus

    largest organelle ineucaryotic cell

    contains geneticmaterial

    enclosed by nuclear

    envelope contains chromatin

    and nucleolus

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    Nucleus

    Chromatin

    dense fibrous materialwithin nucleus

    contains genetic DNA

    condenses to formchromosomes during cell

    division mitosis/meiosis (refer

    Prescotts Microbiology

    pg. 92-94)

    Nuclear envelope

    double membrane (inner

    & outer) structure that

    surrounds nucleus

    has nuclear pores

    pores allow materials

    to be transported intoor out of nucleus

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    Nucleus

    Nucleolus

    1 nucleolus per nucleus

    not membrane-enclosed

    important in ribosome synthesis

    directs synthesis and processing of rRNA directs assembly of rRNA and ribosomal

    proteins to form ribosomes

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    Endoplasmic Reticulum (ER)

    consists of networkof membranous

    tubules andflattened sacs calledcisternae

    internalcompartment of ER= cisternal space

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    Two types of ER:

    rough ER

    ribosomes attached, rough texture

    smooth ER

    lack of ribosomes, smooth texture

    Endoplasmic Reticulum (ER)

    Functions of ER: major site of cell membrane synthesis

    transports proteins, lipids, and other materials to

    Golgi apparatus

    synthesis of lysosomes

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    Golgi Apparatus

    membranous organellemade of cisternae stacked

    on each other dictyosomes

    stacks of cisternae

    involves in modification,packaging, and secretionof materials throughsecretory vesicles

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    cis face (associated with ER)

    trans face

    cis face differs from trans face in thickness,enzyme content and vesicle formation

    movement ofmaterial