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    Chapter 6

    Analysis of Variance

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    Chapter Overview

    Analysis of Variance (ANOVA)

    F-test

    Tukey-Kramer

    test

    One-WayANOVA Two-WayANOVA

    InteractionEffects

    RandomizedBlock Design

    MultipleComparisons

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    General ANOVA Setting

    Investigator controls one or more independentvariables

    factors (the characteristic which differentiates the

    treatment or population from one another) Each factor contains two or more levels (the different

    treatments or population)

    Observe effects on the dependent variable

    Response to levels of independent variable

    Experimental design: the plan used to collectthe data

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    Completely Randomized Design

    Experimental units (subjects) are assignedrandomly to treatments

    Subjects are assumed homogeneous Only one factor or independent variable

    With two or more treatment levels

    Analyzed by one-way analysis of variance(ANOVA)

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    Examples

    An experiment to study the effects of five(levels) different brands of gasoline (factors) onautomobile engine operating efficiency

    An experiment to asses the effects of amounts(factors) of a particular drug on manual dexterity

    Level: different setting of the factor

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    One-Way Analysis of Variance

    Evaluate the difference among the means of threeor more groups

    Examples: Accident rates for 1st, 2nd, and 3rd shift

    Expected mileage for five brands of tires

    Assumptions

    Populations are normally distributed Populations have equal variances

    Samples are randomly and independently drawn

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    Hypotheses of One-Way ANOVA

    All population means are equal

    i.e., no treatment effect (no variation in means among

    groups)

    At least one population mean is different

    i.e., there is a treatment effect

    Does not mean that all population means are different

    (some pairs may be the same)

    c3210 :H

    samethearemeanspopulationtheofallNot:H1

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    Partitioning the Variation

    Total variation can be split into two parts:

    SST = total of the squared deviations ofscores from the overall mean

    (Total variation)SSA = Sum of Squares Among Groups

    (Among-group variation)SSW = Sum of Squares Within Groups

    (Within-group variation)

    SST = SSA + SSW

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    Partitioning the Variation

    Total Variation = the aggregate dispersion of the individualdata values across the various factor levels (SST)

    Within-Group Variation = dispersion that exists amongthe data values within a particular factor level (SSW)

    Among-Group Variation = dispersion between the factorsample means (SSA)

    SST = SSA + SSW

    (continued)

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    Partition of Total Variation

    Variation Due toFactor (SSA)

    Variation Due to RandomSampling (SSW)

    Total Variation (SST)

    Commonly referred to as: Sum of Squares Within

    Sum of Squares Error

    Sum of Squares Unexplained

    Within-Group Variation

    Commonly referred to as: Sum of Squares Between

    Sum of Squares Among

    Sum of Squares Explained

    Among Groups Variation

    = +

    d.f. = n 1

    d.f. = c 1 d.f. = n c

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    Total Sum of Squares

    c

    1j

    n

    1i

    2

    ij

    j

    )XX(SST

    Where:

    SST = Total sum of squares

    c = number of groups (levels or treatments)

    nj = number of observations in group j

    Xij = ith observation from group j

    X = grand mean (mean of all data values)

    SST = SSA + SSW

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    Total Variation

    Group 1 Group 2 Group 3

    Response, X

    X

    2cn

    212

    211 )XX(...)XX()XX(SST c

    (continued)

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    Among-Group Variation

    Where:

    SSA = Sum of squares among groups

    c = number of groups

    nj = sample size from group j

    Xj = sample mean from group j

    X = grand mean (mean of all data values)

    2j

    c

    1j

    j )XX(nSSA

    SST = SSA + SSW

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    Among-Group Variation

    Variation Due toDifferences Among Groups

    i j

    2j

    c

    1j

    j )XX(nSSA

    1cSSAMSA

    Mean Square Among =

    SSA/degrees of freedom

    (continued)

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    Among-Group Variation

    Group 1 Group 2 Group 3

    Response, X

    X1

    X 2X

    3X

    22

    22

    2

    11)xx(n...)xx(n)xx(nSSA cc

    (continued)

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    Within-Group Variation

    Where:

    SSW = Sum of squares within groups

    c = number of groupsnj = sample size from group j

    Xj = sample mean from group j

    Xij

    = ith observation in group j

    2jij

    n

    1i

    c

    1j

    )XX(SSWj

    SST = SSA + SSW

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    Within-Group Variation

    Summing the variationwithin each group and thenadding over all groups cn

    SSWMSW

    Mean Square Within =

    SSW/degrees of freedom

    2jij

    n

    1i

    c

    1j

    )XX(SSWj

    (continued)

    j

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    Within-Group Variation

    Group 1 Group 2 Group 3

    Response, X

    1X

    2X

    3X

    2ccn

    2212

    2111 )XX(...)XX()Xx(SSW c

    (continued)

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    Obtaining the Mean Squares

    cn

    SSWMSW

    1c

    SSAMSA

    1n

    SSTMST

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    One-Way ANOVA Table

    Source ofVariation

    dfSS MS(Variance)

    AmongGroups SSA MSA =

    WithinGroups

    n - cSSW MSW =

    Total n - 1SST =SSA+SSW

    c - 1MSA

    MSW

    F ratio

    c = number of groups

    n = sum of the sample sizes from all groups

    df = degrees of freedom

    SSA

    c - 1

    SSW

    n - c

    F =

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    One-Way ANOVAF Test Statistic

    Test statistic

    MSA is mean squares among groups

    MSWis mean squares within groups

    Degrees of freedom

    df1 = c 1 (c = number of groups)

    df2 = n c (n = sum of sample sizes from all populations)

    MSWMSAF

    H0: 1= 2= = c

    H1: At least two population means are different

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    Interpreting One-Way ANOVAF Statistic

    The F statistic is the ratio of the amongestimate of variance and the within estimateof variance

    The ratio must always be positive df1 = c-1 will typically be small

    df2 = n- c will typically be large

    Decision Rule:

    Reject H0 if F > FU,otherwise do notreject H0

    0

    = .05

    Reject H0Do notreject H0

    FU

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    One-Way ANOVAF Test Example

    You want to see if threedifferent golf clubs yielddifferent distances. You

    randomly select fivemeasurements from trials onan automated drivingmachine for each club. At the

    0.05 significance level, isthere a difference in meandistance?

    Club 1 Club 2 Club 3254 234 200263 218 222

    241 235 197237 227 206251 216 204

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    One-Way ANOVA Example:Scatter Diagram

    270

    260

    250

    240

    230

    220

    210200

    190

    Distance

    1X

    2X

    3X

    X

    227.0x

    205.8x226.0x249.2x 321

    Club 1 Club 2 Club 3254 234 200263 218 222

    241 235 197237 227 206251 216 204

    Club1 2 3

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    F= 25.275

    One-Way ANOVA ExampleSolution

    H0: 1 = 2 = 3

    H1: j not all equal

    = 0.05

    df1= 2 df2 = 12

    Test Statistic:

    Decision:

    Conclusion:

    Reject H0 at = 0.05

    There is evidence thatat least one j differs

    from the rest

    0

    = .05

    FU

    = 3.89Reject H0Do not

    reject H0

    25.275

    93.3

    2358.2

    MSW

    MSAF

    CriticalValue:

    FU

    = 3.89

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    SUMMARY

    Groups Count Sum Average Variance

    Club 1 5 1246 249.2 108.2

    Club 2 5 1130 226 77.5Club 3 5 1029 205.8 94.2

    ANOVA

    Source ofVariation

    SS df MS F P-value F crit

    BetweenGroups

    4716.4 2 2358.2 25.275 4.99E-05 3.89

    WithinGroups

    1119.6 12 93.3

    Total 5836.0 14

    One-Way ANOVAExcel Output

    EXCEL: tools | data analysis | ANOVA: single factor

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    The Tukey-Kramer Procedure

    Tells which population means are significantlydifferent e.g.: 1 = 23

    Done after rejection of equal means in ANOVA

    Allows pair-wise comparisons

    Compare absolute mean differences with criticalrange

    x1 = 2 3

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    Tukey-Kramer Critical Range

    where:

    QU = Value from Studentized Range Distributionwith c and n - c degrees of freedom forthe desired level of

    MSW = Mean Square Within

    nj and nj= Sample sizes from groups j and j

    j'j

    U

    n

    1

    n

    1

    2

    MSWQRangeCritical

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    The Tukey-Kramer Procedure:Example

    1. Compute absolute meandifferences:Club 1 Club 2 Club 3

    254 234 200263 218 222241 235 197237 227 206251 216 204 20.2205.8226.0xx

    43.4205.8249.2xx

    23.2226.0249.2xx

    32

    31

    21

    2. Find the QU value from the table with

    c = 3 and (n c) = (15 3) = 12 degrees of freedomfor the desired level of ( = 0.05 used here):

    3.77QU

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    The Tukey-Kramer Procedure:Example

    5. All of the absolute mean differencesare greater than critical range.Therefore there is a significant

    difference between each pair ofmeans at 5% level of significance.Thus, with 95% confidence we can concludethat the mean distance for club 1 is greaterthan club 2 and 3, and club 2 is greater than

    club 3.

    16.2855

    1

    5

    1

    2

    93.33.77

    n

    1

    n

    1

    2

    MSWQRangeCritical

    j'j

    U

    3. Compute Critical Range:

    20.2xx

    43.4xx

    23.2xx

    32

    31

    21

    4. Compare:

    (continued)

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    The Randomized Block Design

    Like One-Way ANOVA, we test for equalpopulation means (for different factor levels, forexample)...

    ...but we want to control for possible variationfrom a second factor (with two or more levels)

    Levels of the secondary factor are called blocks

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    Partitioning the Variation

    Total variation can now be split into three parts:

    SST = Total variationSSA = Among-Group variationSSBL = Among-Block variationSSE = Random variation

    SST = SSA + SSBL + SSE

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    Sum of Squares for Blocking

    Where:

    c = number of groups

    r = number of blocks

    Xi. = mean of all values in block i

    X = grand mean (mean of all data values)

    r

    1i

    2

    i. )XX(cSSBL

    SST = SSA + SSBL + SSE

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    Partitioning the Variation

    Total variation can now be split into three parts:

    SST and SSA are

    computed as they werein One-Way ANOVA

    SST = SSA + SSBL + SSE

    SSE = SST (SSA + SSBL)

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    Mean Squares

    1c

    SSAgroupsamongsquareMeanMSA

    1r

    SSBLblockingsquareMeanMSBL

    )1)(1(

    cr

    SSEMSE errorsquareMean

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    Randomized Block ANOVA Table

    Source ofVariation

    dfSS MS

    AmongBlocks

    SSBL MSBL

    Error

    (r1)(c-1)SSE MSE

    Total rc - 1SST

    r - 1 MSBL

    MSE

    F ratio

    c = number of populations rc = sum of the sample sizes from all populations

    r = number of blocks df = degrees of freedom

    AmongTreatments SSA c - 1 MSA

    MSA

    MSE

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    Blocking Test

    Blocking test: df1 = r 1

    df2 = (r 1)(c 1)

    MSBL

    MSE

    ...:H 3.2.1.0

    equalaremeansblockallNot:H1

    F =

    Reject H0 if F > FU

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    Main Factor test: df1 = c 1

    df2 = (r 1)(c 1)

    MSA

    MSE

    c..3.2.10...:H

    equalaremeanspopulationallNot:H1

    F =

    Reject H0 if F > FU

    Main Factor Test

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    The Tukey Procedure

    To test which population means are significantlydifferent e.g.: 1 = 23 Done after rejection of equal means in randomized

    block ANOVA design

    Allows pair-wise comparisons Compare absolute mean differences with critical

    range

    x= 1 2 3

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    etc...

    xx

    xx

    xx

    .3.2

    .3.1

    .2.1

    The Tukey Procedure(continued)

    r

    MSERangeCritical uQ

    If the absolute mean differenceis greater than the critical rangethen there is a significantdifference between that pair ofmeans at the chosen level ofsignificance.

    Compare:?RangeCriticalxxIs .j'.j

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    Factorial Design:Two-Way ANOVA

    Examines the effect of

    Two factors of interest on the dependentvariable

    e.g., Percent carbonation and line speed on soft drinkbottling process

    Interaction between the different levels of thesetwo factors

    e.g., Does the effect of one particular carbonationlevel depend on which level the line speed is set?

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    Two-Way ANOVA

    Assumptions

    Populations are normally distributed

    Populations have equal variances

    Independent random samples are

    drawn

    (continued)

    T W ANOVA

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    Two-Way ANOVASources of Variation

    Two Factors of interest: A and B

    r = number of levels of factor A

    c = number of levels of factor B

    n = number of replications for each cell

    n = total number of observations in all cells

    (n = rcn)

    Xijk = value of the kth observation of level i of

    factor A and level j of factor B

    T W ANOVA

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    Two-Way ANOVASources of Variation

    SSTTotal Variation

    SSAFactor A Variation

    SSBFactor B Variation

    SSAB

    Variation due to interactionbetween A and B

    SSERandom variation (Error)

    Degrees ofFreedom:

    r 1

    c 1

    (r 1)(c 1)

    rc(n 1)

    n - 1

    SST = SSA + SSB + SSAB + SSE

    (continued)

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    Two Factor ANOVA Equations

    r

    i

    c

    j

    n

    k

    ijk XXSST1 1 1

    2)(

    2r

    1i

    ..i )XX(ncSSA

    2c

    1j

    .j. )XX(nrSSB

    Total Variation:

    Factor A Variation:

    Factor B Variation:

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    Two Factor ANOVA Equations

    2

    r

    1i

    c

    1j

    .j.i..ij. )XXXX(nSSAB

    r

    1i

    c

    1j

    n

    1k

    2.ijijk )XX(SSE

    Interaction Variation:

    Sum of Squares Error:

    (continued)

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    Two Factor ANOVA Equations

    where:MeanGrand

    nrc

    X

    X

    r

    1i

    c

    1j

    n

    1k

    ijk

    r)...,2,1,(iAfactorofleveliofMeannc

    X

    X th

    c

    1j

    n

    1k

    ijk

    ..i

    c)...,2,1,(jBfactorofleveljofMeannr

    X

    X

    th

    r

    1i

    n

    1k

    ijk

    .j.

    ijcellofMeann

    XX

    n

    1k

    ijk.ij

    r = number of levels of factor A

    c = number of levels of factor B

    n = number of replications in each cell

    (continued)

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    Mean Square Calculations

    1r

    SSAAfactorsquareMeanMSA

    1c

    SSBBfactorsquareMeanMSB

    )1c)(1r(

    SSABninteractiosquareMeanMSAB

    )1'n(rc

    SSEerrorsquareMeanMSE

    T W ANOVA

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    Two-Way ANOVA:The F Test Statistic

    F Test for Factor B Effect

    F Test for Interaction Effect

    H0: 1.. = 2.. = 3..=

    H1: Not all i.. are equal

    H0: the interaction of A and B isequal to zero

    H1: interaction of A and B is notzero

    F Test for Factor A Effect

    H0: .1. = .2. = .3.=

    H1: Not all .j. are equal

    Reject H0

    if F > FUMSE

    MSAF

    MSE

    MSBF

    MSE

    MSABF

    Reject H0

    if F > FU

    Reject H0

    if F > FU

    T W ANOVA

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    Two-Way ANOVASummary Table

    Source ofVariation

    Sum ofSquares

    Degrees ofFreedom

    MeanSquares

    FStatistic

    Factor A SSA r 1MSA

    = SSA/(r 1)

    MSA

    MSE

    Factor B SSB c 1MSB

    = SSB/(c 1)

    MSB

    MSE

    AB

    (Interaction)SSAB (r 1)(c 1)

    MSAB

    = SSAB/ (r 1)(c 1)

    MSAB

    MSE

    Error SSE rc(n 1)MSE =

    SSE/rc(n 1)

    Total SST n 1

    F t f T W ANOVA

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    Features of Two-Way ANOVAFTest

    Degrees of freedom always add up

    n-1 = rc(n-1) + (r-1) + (c-1) + (r-1)(c-1)

    Total = error + factor A + factor B + interaction

    The denominator of the FTest is always the

    same but the numerator is different

    The sums of squares always add up

    SST = SSE + SSA + SSB + SSAB

    Total = error + factor A + factor B + interaction

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    Example

    In a study on automobile traffic and air polution , air sample taken at fourdifferent times and at five different location were analyzed to obtain the

    amount of particulate matter present in the air (mg/m3).

    Is there any difference in a true average amount of particulate matter

    present in the air due to either different sampling times or different location

    E l

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    Examples:Interaction vs. No Interaction

    No interaction:

    Factor B Level 1

    Factor B Level 3

    Factor B Level 2

    Factor A Levels

    Factor B Level 1

    Factor B Level 3

    Factor B Level 2

    Factor A Levels

    MeanResponse

    Me

    anResponse

    Interaction ispresent: