Ch 17: From Gene to Protein

LECTURE PRESENTATIONSFor CAMPBELL BIOLOGY, NINTH EDITION

Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson

© 2011 Pearson Education, Inc.

Lectures byErin Barley

Kathleen Fitzpatrick

From Gene to Protein

Chapter 17

Overview: The Flow of Genetic Information

• The information content of DNA is in the form of specific sequences of nucleotides

• The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins

• Proteins are the links between genotype and phenotype

• Gene expression, the process by which DNA directs protein synthesis, includes two stages: transcription and translation


Figure 17.1

Concept 17.1: Genes specify proteins via transcription and translation

• How was the fundamental relationship between genes and proteins discovered?


Evidence from the Study of Metabolic Defects

• In 1902, British physician Archibald Garrod first suggested that genes dictate phenotypes through enzymes that catalyze specific chemical reactions

• He thought symptoms of an inherited disease reflect an inability to synthesize a certain enzyme

• Linking genes to enzymes required understanding that cells synthesize and degrade molecules in a series of steps, a metabolic pathway


Nutritional Mutants in Neurospora: Scientific Inquiry

• George Beadle and Edward Tatum exposed bread mold to X-rays, creating mutants that were unable to survive on minimal media

• Using crosses, they and their coworkers identified three classes of arginine-deficient mutants, each lacking a different enzyme necessary for synthesizing arginine

• They developed a one gene–one enzyme hypothesis, which states that each gene dictates production of a specific enzyme


Figure 17.2

Minimal medium

No growth:Mutant cellscannot growand divide

Growth:Wild-typecells growingand dividing

EXPERIMENT RESULTS

CONCLUSION

Classes of Neurospora crassa

Wild type Class I mutants Class II mutants Class III mutants

Minimalmedium(MM)(control)

MM +ornithine

MM +citrulline

Co

nd

itio

n

MM +arginine(control)

Summaryof results

Can grow withor without anysupplements

Can grow onornithine,citrulline, orarginine

Can grow onlyon citrulline orarginine

Require arginineto grow

Wild type

Class I mutants(mutation in

gene A)

Class II mutants(mutation in

gene B)

Class III mutants(mutation in

gene C)

Gene (codes forenzyme)

Gene A

Gene B

Gene C

Precursor Precursor Precursor PrecursorEnzyme A Enzyme A Enzyme A Enzyme A

Enzyme B Enzyme B Enzyme B Enzyme B

Enzyme C Enzyme C Enzyme C Enzyme C

Ornithine Ornithine Ornithine Ornithine

Citrulline Citrulline Citrulline Citrulline

Arginine Arginine Arginine Arginine

Figure 17.2a

Minimal medium

No growth:Mutant cellscannot growand divide

Growth:Wild-typecells growingand dividing

EXPERIMENT

Figure 17.2bRESULTS

Classes of Neurospora crassa

Wild type Class I mutants Class II mutants Class III mutants

Minimalmedium(MM)(control)

MM +ornithine

MM +citrullineC

on

dit

ion

MM +arginine(control)

Summaryof results

Can grow withor without anysupplements

Can grow onornithine,citrulline, orarginine

Can grow onlyon citrulline orarginine

Require arginineto grow

Growth Nogrowth

Figure 17.2c

CONCLUSION

Wild type

Class I mutants(mutation in

gene A)

Class II mutants(mutation in

gene B)

Class III mutants(mutation in

gene C)

Gene (codes forenzyme)

Gene A

Gene B

Gene C

Precursor Precursor Precursor PrecursorEnzyme A Enzyme A Enzyme A Enzyme A

Enzyme B Enzyme B Enzyme B Enzyme B

Ornithine Ornithine Ornithine Ornithine

Enzyme C Enzyme C Enzyme CEnzyme C

Citrulline Citrulline Citrulline Citrulline

Arginine Arginine Arginine Arginine

The Products of Gene Expression: A Developing Story

• Some proteins aren’t enzymes, so researchers later revised the hypothesis: one gene–one protein

• Many proteins are composed of several polypeptides, each of which has its own gene

• Therefore, Beadle and Tatum’s hypothesis is now restated as the one gene–one polypeptide hypothesis

• Note that it is common to refer to gene products as proteins rather than polypeptides


Basic Principles of Transcription and Translation

• RNA is the bridge between genes and the proteins for which they code

• Transcription is the synthesis of RNA using information in DNA

• Transcription produces messenger RNA (mRNA)

• Translation is the synthesis of a polypeptide, using information in the mRNA

• Ribosomes are the sites of translation


• In prokaryotes, translation of mRNA can begin before transcription has finished

• In a eukaryotic cell, the nuclear envelope separates transcription from translation

• Eukaryotic RNA transcripts are modified through RNA processing to yield the finished mRNA


• A primary transcript is the initial RNA transcript from any gene prior to processing

• The central dogma is the concept that cells are governed by a cellular chain of command: DNA → RNA → protein


Figure 17.UN01

DNA RNA Protein

Figure 17.3

DNA

mRNARibosome

Polypeptide

TRANSCRIPTION

TRANSLATION

TRANSCRIPTION

TRANSLATION

Polypeptide

Ribosome

DNA

mRNA

Pre-mRNARNA PROCESSING

(a) Bacterial cell (b) Eukaryotic cell

Nuclearenvelope

Figure 17.3a-1

TRANSCRIPTIONDNA

mRNA

(a) Bacterial cell

Figure 17.3a-2

TRANSCRIPTIONDNA

mRNA

(a) Bacterial cell

TRANSLATIONRibosome

Polypeptide

Figure 17.3b-1

Nuclearenvelope

DNA

Pre-mRNA

(b) Eukaryotic cell

TRANSCRIPTION

Figure 17.3b-2

RNA PROCESSING

Nuclearenvelope

DNA

Pre-mRNA

(b) Eukaryotic cell

mRNA

TRANSCRIPTION

Figure 17.3b-3

RNA PROCESSING

Nuclearenvelope

DNA

Pre-mRNA

(b) Eukaryotic cell

mRNA

TRANSCRIPTION

TRANSLATION Ribosome

Polypeptide

The Genetic Code

• How are the instructions for assembling amino acids into proteins encoded into DNA?

• There are 20 amino acids, but there are only four nucleotide bases in DNA

• How many nucleotides correspond to an amino acid?


Codons: Triplets of Nucleotides

• The flow of information from gene to protein is based on a triplet code: a series of nonoverlapping, three-nucleotide words

• The words of a gene are transcribed into complementary nonoverlapping three-nucleotide words of mRNA

• These words are then translated into a chain of amino acids, forming a polypeptide


Figure 17.4

DNAtemplatestrand

TRANSCRIPTION

mRNA

TRANSLATION

Protein

Amino acid

Codon

Trp Phe Gly

5′

5′

Ser

U U U U U3′

3′

5′3′

G

G

G G C C

T

C

A

A

AAAAA

T T T T

T

G

G G G

C C C G G

DNAmolecule

Gene 1

Gene 2

Gene 3

C C

• During transcription, one of the two DNA strands, called the template strand, provides a template for ordering the sequence of complementary nucleotides in an RNA transcript

• The template strand is always the same strand for a given gene

• During translation, the mRNA base triplets, called codons, are read in the 5′ to 3′ direction


• Codons along an mRNA molecule are read by translation machinery in the 5′ to 3′ direction

• Each codon specifies the amino acid (one of 20) to be placed at the corresponding position along a polypeptide


Cracking the Code

• All 64 codons were deciphered by the mid-1960s• Of the 64 triplets, 61 code for amino acids; 3

triplets are “stop” signals to end translation• The genetic code is redundant (more than one

codon may specify a particular amino acid) but not ambiguous; no codon specifies more than one amino acid

• Codons must be read in the correct reading frame (correct groupings) in order for the specified polypeptide to be produced


Figure 17.5Second mRNA base

Fir

st m

RN

A b

ase

(5′ e

nd

of

cod

on

)

Th

ird

mR

NA

bas

e (3

′ en

d o

f co

do

n)

UUU

UUC

UUA

CUU

CUC

CUA

CUG

Phe

Leu

Leu

Ile

UCU

UCC

UCA

UCG

Ser

CCU

CCC

CCA

CCG

UAU

UACTyr

Pro

Thr

UAA Stop

UAG Stop

UGA Stop

UGU

UGCCys

UGG Trp

GC

U

U

C

A

U

U

C

C

CA

U

A

A

A

G

G

His

Gln

Asn

Lys

Asp

CAU CGU

CAC

CAA

CAG

CGC

CGA

CGG

G

AUU

AUC

AUA

ACU

ACC

ACA

AAU

AAC

AAA

AGU

AGC

AGA

Arg

Ser

Arg

Gly

ACGAUG AAG AGG

GUU

GUC

GUA

GUG

GCU

GCC

GCA

GCG

GAU

GAC

GAA

GAG

Val Ala

GGU

GGC

GGA

GGGGlu

Gly

G

U

C

A

Met orstart

UUG

G

Evolution of the Genetic Code

• The genetic code is nearly universal, shared by the simplest bacteria to the most complex animals

• Genes can be transcribed and translated after being transplanted from one species to another


Figure 17.6

(a) Tobacco plant expressing a firefly gene gene

(b) Pig expressing a jellyfish

Figure 17.6a

(a) Tobacco plant expressinga firefly gene

Figure 17.6b

(b) Pig expressing a jellyfishgene

Concept 17.2: Transcription is the DNA-directed synthesis of RNA: a closer look

• Transcription is the first stage of gene expression


Molecular Components of Transcription

• RNA synthesis is catalyzed by RNA polymerase, which pries the DNA strands apart and hooks together the RNA nucleotides

• The RNA is complementary to the DNA template strand

• RNA synthesis follows the same base-pairing rules as DNA, except that uracil substitutes for thymine


• The DNA sequence where RNA polymerase attaches is called the promoter; in bacteria, the sequence signaling the end of transcription is called the terminator

• The stretch of DNA that is transcribed is called a transcription unit


Animation: Transcription

Figure 17.7-1 Promoter

RNA polymeraseStart point

DNA

5′3′

Transcription unit

3′5′



DNA

5′3′

Transcription unit

3′5′

Initiation

5′3′

3′5′

Nontemplate strand of DNA

Template strand of DNARNAtranscriptUnwound

DNA

1



DNA

5′3′

Transcription unit

3′5′

Elongation

5′3′

3′5′



DNA2

3′5′3′5′

3′

RewoundDNA

RNAtranscript

5′

Initiation1



DNA

5′3′

Transcription unit

3′5′

Elongation

5′3′

3′5′



DNA2

3′5′3′5′

3′

RewoundDNA

RNAtranscript

5′

Termination3

3′5′

5′Completed RNA transcript

Direction of transcription (“downstream”)

5′3′

3′

Initiation1

Synthesis of an RNA Transcript

• The three stages of transcription– Initiation

– Elongation– Termination


RNA Polymerase Binding and Initiation of Transcription

• Promoters signal the transcriptional start point and usually extend several dozen nucleotide pairs upstream of the start point

• Transcription factors mediate the binding of RNA polymerase and the initiation of transcription

• The completed assembly of transcription factors and RNA polymerase II bound to a promoter is called a transcription initiation complex

• A promoter called a TATA box is crucial in forming the initiation complex in eukaryotes


Figure 17.8

Transcription initiationcomplex forms

3

DNAPromoter

Nontemplate strand

5′3′

5′3′

5′3′

Transcriptionfactors

RNA polymerase II

Transcription factors

5′3′

5′3′

5′3′

RNA transcript

Transcription initiation complex

5′ 3′

TATA box

T

T T T T T

A A A AA

A A

T

Several transcriptionfactors bind to DNA

2

A eukaryotic promoter1

Start point Template strand

Elongation of the RNA Strand

• As RNA polymerase moves along the DNA, it untwists the double helix, 10 to 20 bases at a time

• Transcription progresses at a rate of 40 nucleotides per second in eukaryotes

• A gene can be transcribed simultaneously by several RNA polymerases

• Nucleotides are added to the 3′ end of the growing RNA molecule


Nontemplatestrand of DNA

RNA nucleotides

RNApolymerase

Templatestrand of DNA

3′

3′5′

5′

5′

3′

Newly madeRNA

Direction of transcription

A

A A A

AA

A

T

TT

T

TTT G

GG

C

C C

CC

G

C CC A AA

U

U

U

end

Figure 17.9

Termination of Transcription

• The mechanisms of termination are different in bacteria and eukaryotes

• In bacteria, the polymerase stops transcription at the end of the terminator and the mRNA can be translated without further modification

• In eukaryotes, RNA polymerase II transcribes the polyadenylation signal sequence; the RNA transcript is released 10–35 nucleotides past this polyadenylation sequence


Concept 17.3: Eukaryotic cells modify RNA after transcription

• Enzymes in the eukaryotic nucleus modify pre-mRNA (RNA processing) before the genetic messages are dispatched to the cytoplasm

• During RNA processing, both ends of the primary transcript are usually altered

• Also, usually some interior parts of the molecule are cut out, and the other parts spliced together


Alteration of mRNA Ends

• Each end of a pre-mRNA molecule is modified in a particular way

– The 5′ end receives a modified nucleotide 5′ cap

– The 3′ end gets a poly-A tail• These modifications share several functions

– They seem to facilitate the export of mRNA to the cytoplasm

– They protect mRNA from hydrolytic enzymes– They help ribosomes attach to the 5′ end


Figure 17.10

Protein-codingsegment

Polyadenylationsignal

5′ 3′

3′5′ 5′Cap UTRStartcodon

G P P P

Stopcodon

UTR

AAUAAA

Poly-A tail

AAA AAA…

Split Genes and RNA Splicing

• Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions

• These noncoding regions are called intervening sequences, or introns

• The other regions are called exons because they are eventually expressed, usually translated into amino acid sequences

• RNA splicing removes introns and joins exons, creating an mRNA molecule with a continuous coding sequence


Figure 17.11

5′ Exon Intron Exon

5′ CapPre-mRNACodonnumbers

1−30 31−104

mRNA 5′ Cap

5′

Intron Exon

3′ UTR

Introns cut out andexons spliced together

3′

105− 146

Poly-A tail

Codingsegment

Poly-A tail

UTR1−146

• In some cases, RNA splicing is carried out by spliceosomes

• Spliceosomes consist of a variety of proteins and several small nuclear ribonucleoproteins (snRNPs) that recognize the splice sites


Figure 17.12-1RNA transcript (pre-mRNA)

5′Exon 1

Protein

snRNA

snRNPs

Intron Exon 2

Other proteins


5′Exon 1

Protein

snRNA

snRNPs

Intron Exon 2

Other proteins

Spliceosome

5′


5′Exon 1

Protein

snRNA

snRNPs

Intron Exon 2

Other proteins

Spliceosome

5′

Spliceosomecomponents

Cut-outintronmRNA

5′Exon 1 Exon 2

Ribozymes

• Ribozymes are catalytic RNA molecules that function as enzymes and can splice RNA

• The discovery of ribozymes rendered obsolete the belief that all biological catalysts were proteins


• Three properties of RNA enable it to function as an enzyme

– It can form a three-dimensional structure because of its ability to base-pair with itself

– Some bases in RNA contain functional groups that may participate in catalysis

– RNA may hydrogen-bond with other nucleic acid molecules


The Functional and Evolutionary Importance of Introns

• Some introns contain sequences that may regulate gene expression

• Some genes can encode more than one kind of polypeptide, depending on which segments are treated as exons during splicing

• This is called alternative RNA splicing• Consequently, the number of different proteins an

organism can produce is much greater than its number of genes


• Proteins often have a modular architecture consisting of discrete regions called domains

• In many cases, different exons code for the different domains in a protein

• Exon shuffling may result in the evolution of new proteins


GeneDNA

Exon 1 Exon 2 Exon 3Intron Intron

Transcription

RNA processing

Translation

Domain 3

Domain 2

Domain 1

Polypeptide

Figure 17.13

Concept 17.4: Translation is the RNA-directed synthesis of a polypeptide: a closer look

• Genetic information flows from mRNA to protein through the process of translation


Molecular Components of Translation

• A cell translates an mRNA message into protein with the help of transfer RNA (tRNA)

• tRNAs transfer amino acids to the growing polypeptide in a ribosome

• Translation is a complex process in terms of its biochemistry and mechanics


Figure 17.14

Polypeptide

Ribosome

Trp

Phe Gly

tRNA withamino acidattached

Aminoacids

tRNA

Anticodon

Codons

U U U UG G G G C

AC C

C

CG

A A A

CGC

G

5′ 3′mRNA

The Structure and Function of Transfer RNA

• Molecules of tRNA are not identical– Each carries a specific amino acid on one end

– Each has an anticodon on the other end; the anticodon base-pairs with a complementary codon on mRNA


BioFlix: Protein Synthesis

• A tRNA molecule consists of a single RNA strand that is only about 80 nucleotides long

• Flattened into one plane to reveal its base pairing, a tRNA molecule looks like a cloverleaf


Figure 17.15

Amino acidattachmentsite

3′

5′

Hydrogenbonds

Anticodon

(a) Two-dimensional structure (b) Three-dimensional structure(c) Symbol used

in this book

Anticodon Anticodon3′ 5′

Hydrogenbonds

Amino acidattachmentsite5′

3′

A A G

Figure 17.15a

Amino acidattachmentsite

3′

5′

Hydrogenbonds

Anticodon

(a) Two-dimensional structure

(b) Three-dimensional structure(c) Symbol used

Anticodon Anticodon3′ 5′

Hydrogenbonds

Amino acidattachmentsite5′

3′

in this book

A A G

Figure 17.15b

• Because of hydrogen bonds, tRNA actually twists and folds into a three-dimensional molecule

• tRNA is roughly L-shaped


• Accurate translation requires two steps– First: a correct match between a tRNA and an

amino acid, done by the enzyme aminoacyl-tRNA synthetase

– Second: a correct match between the tRNA anticodon and an mRNA codon

• Flexible pairing at the third base of a codon is called wobble and allows some tRNAs to bind to more than one codon


Aminoacyl-tRNAsynthetase (enzyme)

Amino acid

P P P Adenosine

ATP

Figure 17.16-1


Amino acid

P P P Adenosine

ATP

P

P

P

PPi

i

i

Adenosine

Figure 17.16-2


Amino acid

P P P Adenosine

ATP

P

P

P

PPi

i

i

Adenosine

tRNA

AdenosineP

tRNA

AMP

Computer model

Aminoacid

Aminoacyl-tRNAsynthetase

Figure 17.16-3


Amino acid

P P P Adenosine

ATP

P

P

P

PPi

i

i

Adenosine

tRNA

AdenosineP

tRNA

AMP

Computer model

Aminoacid

Aminoacyl-tRNAsynthetase

Aminoacyl tRNA(“charged tRNA”)

Figure 17.16-4

Ribosomes

• Ribosomes facilitate specific coupling of tRNA anticodons with mRNA codons in protein synthesis

• The two ribosomal subunits (large and small) are made of proteins and ribosomal RNA (rRNA)

• Bacterial and eukaryotic ribosomes are somewhat similar but have significant differences: some antibiotic drugs specifically target bacterial ribosomes without harming eukaryotic ribosomes


tRNAmolecules

Growingpolypeptide Exit tunnel

E PA

Largesubunit

Smallsubunit

mRNA5′

3′

(a) Computer model of functioning ribosome

Exit tunnel Amino end

A site (Aminoacyl-tRNA binding site)

Smallsubunit

Largesubunit

E P AmRNA

E

P site (Peptidyl-tRNAbinding site)

mRNAbinding site

(b) Schematic model showing binding sites

E site (Exit site)

(c) Schematic model with mRNA and tRNA

5′ Codons

3′tRNA

Growing polypeptide

Next aminoacid to beadded topolypeptidechain

Figure 17.17

Figure 17.17a

tRNAmolecules

Growingpolypeptide Exit tunnel

E P A

Largesubunit

Smallsubunit

mRNA5′

3′

(a) Computer model of functioning ribosome

Figure 17.17b

Exit tunnel

A site (Aminoacyl-tRNA binding site)

Smallsubunit

Largesubunit

P A

P site (Peptidyl-tRNAbinding site)

mRNAbinding site

(b) Schematic model showing binding sites

E site (Exit site)

E

Figure 17.17c

Amino end

mRNAE

(c) Schematic model with mRNA and tRNA

5′ Codons

3′tRNA

Growing polypeptide

Next aminoacid to beadded topolypeptidechain

• A ribosome has three binding sites for tRNA– The P site holds the tRNA that carries the

growing polypeptide chain

– The A site holds the tRNA that carries the next amino acid to be added to the chain

– The E site is the exit site, where discharged tRNAs leave the ribosome


Building a Polypeptide

• The three stages of translation– Initiation– Elongation

– Termination

• All three stages require protein “factors” that aid in the translation process


Ribosome Association and Initiation of Translation

• The initiation stage of translation brings together mRNA, a tRNA with the first amino acid, and the two ribosomal subunits

• First, a small ribosomal subunit binds with mRNA and a special initiator tRNA

• Then the small subunit moves along the mRNA until it reaches the start codon (AUG)

• Proteins called initiation factors bring in the large subunit that completes the translation initiation complex


Figure 17.18

InitiatortRNA

mRNA

5′

5′3′Start codon

Smallribosomalsubunit

mRNA binding site

3′

Translation initiation complex

5′ 3′3′ U

UA

A GC

P

P site

i+

GTP GDP

Met Met

Largeribosomalsubunit

E A

5′

Elongation of the Polypeptide Chain

• During the elongation stage, amino acids are added one by one to the preceding amino acid at the C-terminus of the growing chain

• Each addition involves proteins called elongation factors and occurs in three steps: codon recognition, peptide bond formation, and translocation

• Translation proceeds along the mRNA in a 5′ to 3′ direction


Amino end ofpolypeptide

mRNA

5′

E

Psite

Asite

3′

Figure 17.19-1


mRNA

5′

E

Psite

Asite

3′

E

GTP

GDP + P i

P A

Figure 17.19-2


mRNA

5′

E

Psite

Asite

3′

E

GTP

GDP + P i

P A

E

P A

Figure 17.19-3


mRNA

5′

E

Asite

3′

E

GTP

GDP + P i

P A

E

P A

GTP

GDP + P i

P A

E

Ribosome ready fornext aminoacyl tRNA

Psite

Figure 17.19-4

Termination of Translation

• Termination occurs when a stop codon in the mRNA reaches the A site of the ribosome

• The A site accepts a protein called a release factor

• The release factor causes the addition of a water molecule instead of an amino acid

• This reaction releases the polypeptide, and the translation assembly then comes apart


Animation: Translation

Figure 17.20-1

Releasefactor

Stop codon(UAG, UAA, or UGA)

3′

5′

Figure 17.20-2

Releasefactor


3′

5′

3′

5′

Freepolypeptide

2 GTP

2 GDP + 2 iP

Figure 17.20-3

Releasefactor


3′

5′

3′

5′

Freepolypeptide

2 GTP

5′

3′

2 GDP + 2 iP

Polyribosomes

• A number of ribosomes can translate a single mRNA simultaneously, forming a polyribosome (or polysome)

• Polyribosomes enable a cell to make many copies of a polypeptide very quickly


Figure 17.21Completedpolypeptide

Incomingribosomalsubunits

Start ofmRNA(5′ end)

End ofmRNA(3′ end)(a)

Polyribosome

Ribosomes

mRNA

(b)0.1 µm

Growingpolypeptides

Figure 17.21a

Ribosomes

mRNA

0.1 µm

Completing and Targeting the Functional Protein

• Often translation is not sufficient to make a functional protein

• Polypeptide chains are modified after translation or targeted to specific sites in the cell


Protein Folding and Post-Translational Modifications

• During and after synthesis, a polypeptide chain spontaneously coils and folds into its three-dimensional shape

• Proteins may also require post-translational modifications before doing their job

• Some polypeptides are activated by enzymes that cleave them

• Other polypeptides come together to form the subunits of a protein


Targeting Polypeptides to Specific Locations

• Two populations of ribosomes are evident in cells: free ribsomes (in the cytosol) and bound ribosomes (attached to the ER)

• Free ribosomes mostly synthesize proteins that function in the cytosol

• Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cell

• Ribosomes are identical and can switch from free to bound


• Polypeptide synthesis always begins in the cytosol

• Synthesis finishes in the cytosol unless the polypeptide signals the ribosome to attach to the ER

• Polypeptides destined for the ER or for secretion are marked by a signal peptide


• A signal-recognition particle (SRP) binds to the signal peptide

• The SRP brings the signal peptide and its ribosome to the ER


Figure 17.22

Ribosome

mRNA

Signalpeptide

SRP

1

SRPreceptorprotein

Translocationcomplex

ERLUMEN

2

3

45

6

Signalpeptideremoved

CYTOSOL

Protein

ERmembrane

Concept 17.5: Mutations of one or a few nucleotides can affect protein structure and function

• Mutations are changes in the genetic material of a cell or virus

• Point mutations are chemical changes in just one base pair of a gene

• The change of a single nucleotide in a DNA template strand can lead to the production of an abnormal protein


Figure 17.23

Wild-type hemoglobin

Wild-type hemoglobin DNA3′

3′

3′5′

5′ 3′

3′5′

5′5′5′3′

mRNA

A AGC T T

A AGmRNA

Normal hemoglobin

Glu

Sickle-cell hemoglobin

Val

AA

AUG

GT

T

Sickle-cell hemoglobin

Mutant hemoglobin DNAC

Types of Small-Scale Mutations

• Point mutations within a gene can be divided into two general categories

– Nucleotide-pair substitutions– One or more nucleotide-pair insertions or

deletions


Substitutions

• A nucleotide-pair substitution replaces one nucleotide and its partner with another pair of nucleotides

• Silent mutations have no effect on the amino acid produced by a codon because of redundancy in the genetic code

• Missense mutations still code for an amino acid, but not the correct amino acid

• Nonsense mutations change an amino acid codon into a stop codon, nearly always leading to a nonfunctional protein


Wild type

DNA template strand

mRNA5′

5′

3′

Protein

Amino end

A instead of G

(a) Nucleotide-pair substitution

3′

3′

5′

Met Lys Phe Gly StopCarboxyl end

T T T T T

TTTTTA A A A A

AAAACC

C

C

A

A A A A A

G G G G

GC C

G GGU U U U UG

(b) Nucleotide-pair insertion or deletion

Extra A

3′5′

5′3′

Extra U

5′ 3′

T T T T

T T T T

A

A A A

A

AT G G G G

GAAA

AC

CCCC A

T3′5′

5′ 3′

5′T T T T TAAAACCA AC C

TTTTTA A A A ATG G G G

U instead of C

Stop

UA A A A AG GGU U U U UG

MetLys Phe Gly

Silent (no effect on amino acid sequence)

T instead of C

T T T T TAAAACCA GT C

T A T T TAAAACCA GC C

A instead of G

CA A A A AG AGU U U U UG UA A A AG GGU U U G AC

AA U U A AU UGU G G C UA

GA U A U AA UGU G U U CG

Met Lys Phe Ser

Stop

Stop Met Lys

missing

missing

Frameshift causing immediate nonsense(1 nucleotide-pair insertion)

Frameshift causing extensive missense (1 nucleotide-pair deletion)

missing

T T T T TTCAACCA AC G

AGTTTA A A A ATG G G C

Leu Ala

Missense

A instead of T

TTTTTA A A A ACG G A G

A

CA U A A AG GGU U U U UG

TTTTTA T A A ACG G G G

Met

Nonsense

Stop

U instead of A

3′5′

3′5′

5′3′

3′5′

5′3′

3′5′ 3′Met Phe Gly

No frameshift, but one amino acid missing(3 nucleotide-pair deletion)

missing

3′5′

5′3′

5′ 3′U

T CA AA CA TTAC G

TA G T T T G G A ATC

T T C

A A G

Met

3′

T

A

Stop

3′5′

5′3′

5′ 3′

Figure 17.24

Figure 17.24a

Wild type

DNA template strand

mRNA5′

5′

Protein

Amino endStopCarboxyl end

3′3′

3′

5′

Met Lys Phe Gly

A instead of G

(a) Nucleotide-pair substitution: silent

StopMet Lys Phe Gly

U instead of C

A

A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

C

C

G G G G

G

G

A

A A A AG GGU U U U U

5′3′

3′5′A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

G G G G

A

A

A G A A A AG GGU U U U U

T

U 3′5′

Figure 17.24b

Wild type

DNA template strand

mRNA5′

5′

Protein


3′3′

3′

5′

Met Lys Phe Gly

T instead of C

(a) Nucleotide-pair substitution: missense

StopMet Lys Phe Ser

A instead of G

A

A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

C

C

G G G G

G

G

A


5′3′

3′5′A

A A

A A A A

A AT

T T T T T

T T TT

C C T C

G

G

GA

A G A A A AA GGU U U U U 3′5′

A C

C

G

Figure 17.24c

Wild type

DNA template strand

mRNA5′

5′

Protein


3′3′

3′

5′

Met Lys Phe Gly

A instead of T

(a) Nucleotide-pair substitution: nonsense

Met

A

A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

C

C

G G G G

G

G

A


5′3′

3′5′A

A

A A A A

A AT

T A T T T

T T TT

C C C

G

G

GA

A G U A A AGGU U U U U 3′5′

C

C

G

T instead of C

C

GT

U instead of A

G

Stop

Insertions and Deletions

• Insertions and deletions are additions or losses of nucleotide pairs in a gene

• These mutations have a disastrous effect on the resulting protein more often than substitutions do

• Insertion or deletion of nucleotides may alter the reading frame, producing a frameshift mutation


Figure 17.24d

Wild type

DNA template strand

mRNA5′

5′

Protein


3′3′

3′

5′

Met Lys Phe Gly

A

A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

C

C

G G G G

G

G

A


(b) Nucleotide-pair insertion or deletion: frameshift causingimmediate nonsense

Extra A

Extra U

5′3′

5′

3′

3′

5′

Met

1 nucleotide-pair insertion

Stop

A C A A GT T A TC T A C G

T A T AT G T CT GG A T GA

A G U A U AU GAU G U U C

A T

A

AG

Figure 17.24e

DNA template strand

mRNA5′

5′

Protein


3′3′

3′

5′

Met Lys Phe Gly

A

A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

C

C

G G G G

G

G

A


(b) Nucleotide-pair insertion or deletion: frameshift causingextensive missense

Wild type

missing

missing

A

U

A A AT T TC C A T TC C G

A AT T TG GA A ATCG G

A G A A GU U U C A AG G U 3′

5′3′

3′5′

Met Lys Leu Ala

1 nucleotide-pair deletion

5′

Figure 17.24f

DNA template strand

mRNA5′

5′

Protein


3′3′

3′

5′

Met Lys Phe Gly

A

A

A A

A A A A

A AT

T T T T T

T T TT

C C C C

C

C

G G G G

G

G

A


(b) Nucleotide-pair insertion or deletion: no frameshift, but oneamino acid missing

Wild type

AT C A A A A T TC C G

T T C missing

missing

Stop

5′3′

3′5′

3′5′

Met Phe Gly

3 nucleotide-pair deletion

A GU C A AG GU U U U

T GA A AT T TT CG G

A A G

Mutagens

• Spontaneous mutations can occur during DNA replication, recombination, or repair

• Mutagens are physical or chemical agents that can cause mutations


Concept 17.6: While gene expression differs among the domains of life, the concept of a gene is universal

• Archaea are prokaryotes, but share many features of gene expression with eukaryotes


Comparing Gene Expression in Bacteria, Archaea, and Eukarya

• Bacteria and eukarya differ in their RNA polymerases, termination of transcription, and ribosomes; archaea tend to resemble eukarya in these respects

• Bacteria can simultaneously transcribe and translate the same gene

• In eukarya, transcription and translation are separated by the nuclear envelope

• In archaea, transcription and translation are likely coupled


Figure 17.25

RNA polymerase

DNAmRNA

Polyribosome

RNA polymerase DNA

Polyribosome

Polypeptide(amino end)

mRNA (5′ end)

Ribosome

0.25 µmDirection oftranscription

Figure 17.25a

RNA polymerase

DNAmRNA

Polyribosome

0.25 µm

What Is a Gene? Revisiting the Question

• The idea of the gene has evolved through the history of genetics

• We have considered a gene as– A discrete unit of inheritance

– A region of specific nucleotide sequence in a chromosome

– A DNA sequence that codes for a specific polypeptide chain


Figure 17.26TRANSCRIPTION

DNA

RNApolymerase

ExonRNAtranscript

RNAPROCESSING

NUCLEUS

Intron

RNA transcript(pre-mRNA)

Poly-A

Poly-

A

Aminoacyl-tRNA synthetase

AMINO ACIDACTIVATION

Aminoacid

tRNA

5′ Cap

Poly-A

3′

GrowingpolypeptidemRNA

Aminoacyl(charged)tRNA

Anticodon

Ribosomalsubunits

A

AETRANSLATION

5′ Cap

CYTOPLASM

P

E

Codon

Ribosome

5′

3′

• In summary, a gene can be defined as a region of DNA that can be expressed to produce a final functional product, either a polypeptide or an RNA molecule

© 2011 Pearson Education, Inc.© 2011 Pearson Education, Inc.

Figure 17.UN02

Transcription unit

RNA polymerase

Promoter

RNA transcript

5′

5′3′

3′ 3′5′

Template strandof DNA

Figure 17.UN03

Pre-mRNA

mRNA

Poly-A tail5′ Cap

Figure 17.UN04

tRNA

Polypeptide

Aminoacid

E A Anti-codon

Ribosome mRNACodon

Figure 17.UN05

Type of RNA Functions

Messenger RNA (mRNA)

Transfer RNA (tRNA)

Primary transcript

Small nuclear RNA (snRNA)

Plays catalytic (ribozyme) roles andstructural roles in ribosomes

Figure 17.UN06a

Figure 17.UN06b

Figure 17.UN06c

Figure 17.UN06d

Figure 17.UN10

Ch 17: From Gene to Protein

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