CellEase II Series - Amazon S3 · CellEase ® II Series For fast and simple DNA extraction Blood...
Transcript of CellEase II Series - Amazon S3 · CellEase ® II Series For fast and simple DNA extraction Blood...
CellEase® II SeriesFor fast and simple DNA extraction
Blood
Plant
MeatTissue
Bacteria
BloBBBlBlolo
Features and Advantages
● Rapid and efficient DNA extraction ● Easy and simple protocol to prepare PCR grade template DNA ● Low cost ● Use for research and laboratory food industry
① 100 μl of CellEase® mixtureReagent A 20 μlReagent B 20 μlDistilled water 60 μl
② 2~10 mg sample
Mix CellEase® mixture and sample
Incubate at specified temperatures72℃, 6 min94℃, 3 min
Use 1~10 μl of extracted DNA as a template for PCR(Total 50 μl reaction volume)
Applicable Samples:Tissue, Bacteria, Meat, Blood and plants.
R grade template DNA
ustry
CellEase Mix
Distilled water
PCR
Phenolreagent
Centrifugalseparation
Ethanolprecipitation
Alkalinereagent
ConventionalCellEase
Generalmethod
Sample
CellEase® II Mix
Centrifugalseparation
Only 1 step +
CellEase® II Series For fast and simple DNA extraction
DNA Extraction Examples
Ordering Information
DNA Extraction Examples
DNA extraction from mouse tail
M:Marker (100 bp ladder)1: DNA extract by using CellEase® TissueⅡ conc.2: DNA extract by using CellEase® TissueⅡ conc.×103: DNA extract by using CellEase® TissueⅡ conc.×1004: DNA extract by using conventional CellEase® conc.5: DNA extract by using conventional CellEase® conc.×106: DNA extract by using conventional CellEase® conc.×100
DNA extraction from tomato leaf
M: Marker(500 bp ladder)1: + CellEase®, Without dilution Add 5 μl to PCR2: + CellEase®, ×10 dilution Add 5 μl to PCR3: + CellEase®, × 100 dilution Add 5 μl to PCR4: ‐ CellEase®, Without dilution Add 5 μl to PCR5: ‐ CellEase®, ×10 dilution Add 5 μl to PCR6: ‐ CellEase®, × 100 dilution Add 5 μl to PCR7: + CellEase®, Without dilution Add 6 μl to PCR8: + CellEase®, ×10 dilution Add 6 μl to PCR9: + CellEase®, × 100dilution Add 6 μl to PCR
10: ‐ CellEase®, Without dilution Add 6 μl to PCR11: ‐ CellEase®,×10 dilution Add 6 μl to PCR12: ‐ CellEase®,× 100 dilution Add 6 μl to PCR13: + CellEase®, Without dilution Add 7 μl to PCR14: + CellEase®, ×10 dilution Add 7 μl to PCR15: + CellEase®, × 100 dilution Add 7 μl to PCR16: ‐ CellEase®, Without dilution Add 7 μl to PCR17: ‐ CellEase®, ×10 dilution Add 7 μl to PCR18: ‐ CellEase®, × 100 dilution Add 7 μl to PCR
DNA extraction from Staphylococcus. aureus
M Marker (500 bp ladder)1: Undiluted Sample Add 5 μl of DNA extract to PCR2: x 10 dilution3: x 102 dilution4: x 103 dilution5: x 104 dilution6: Undiluted Sample Add 6 μl of DNA extract to PCR7: x 10 dilution8: x 102 dilution9: x 103 dilution10: x 104 dilution
DNA extraction from whole blood
Sample: Whole blood (EDTA)M: Marker (100 bp ladder)1: CellEase® Blood2: CellEase® Blood 3: CellEase® Blood 4: Extraction by water
DNA extraction from fish (Tuna) samples
M: Marker(100 bp ladder)1: Sample 6 mg, Add 5 μl of DNA extract to PCR2: Sample 6 mg, Add 6 μl of DNA extract to PCR3: Sample 6 mg, Add 7 μl of DNA extract to PCR4: Sample 9 mg, Add 5 μl of DNA extract to PCR5: Sample 9 mg, Add 6 μl of DNA extract to PCR6: Sample 9 mg, Add 7 μl of DNA extract to PCR7: Sample 18 mg, Add 5 μl of DNA extract to PCR8: Sample 18 mg Add 6 μl of DNA extract to PCR9: Sample 18 mg Add 7 μl of DNA extract to PCR
Product Name Catalog Number Size Storage
CellEase® Tissue II BIC-BCR10-00001
CellEase® Bacteria II BIC-BCR10-00002
CellEase® Meat II BIC-BCR10-00003 50 Extractions 4℃
CellEase® Blood BIC-BCR11-00001
CellEase® Plant BIC-BCR11-00002
10080For research use only, Not for diagnostic use. www.cosmobio.com
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