Cell injury, apotosis and necrosis(1)
-
Upload
university-of-faisalabad -
Category
Technology
-
view
145 -
download
1
description
Transcript of Cell injury, apotosis and necrosis(1)
![Page 1: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/1.jpg)
CELL INJURY, APOPTOSIS AND NECROSIS
![Page 2: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/2.jpg)
CONCEPTS IN CELL INJURY
• Cell injury results from a disruption of one or more of the cellular components that maintain cell viability.
• Cell injury is common to all pathologic processes.
![Page 3: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/3.jpg)
CONCEPTS IN CELL INJURY
• Cell injury may be reversible, result in cell adaptation, or lead to cell death.
• Injury at one point induces a cascade of effects.
![Page 4: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/4.jpg)
CAUSES OF CELL INJURY -THE PATIENT’S VIEW
• Hypoxia
• Infectious agents
• Physical injury
• Chemicals/drugs
• Immune response
• Genetic derangement
• Nutritional imbalance
![Page 5: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/5.jpg)
• Ischemia ("ischemic hypoxia"; "stagnant hypoxia"): Loss of arterial blood flow (* literally, "holding back the blood")
• Local causes – Occlusion of the arteries that bring in fresh blood – Occlusion of the veins which allow blood to leave, so
that fresh blood can flow in – Shunting of arterial blood elsewhere ("steal
syndromes"; "Robin Hood" syndromes)
• Systemic causes – Failure of the heart to pump enough blood
![Page 6: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/6.jpg)
Hypoxemia: Too little available oxygen in the blood
Oxygen problems ("hypoxic hypoxia") Too little oxygen in the air Failure to properly ventilate the lungs Failure of the lungs to properly oxygenate the blood Failure of the heart to pump enough blood through the lungs Tremendously increased dead space (i.e., pulmonary thromboembolus)
Hemoglobin problems ("anemic hypoxia") Inadequate circulating red cell mass ("anemia") Inability of hemoglobin to carry the oxygen (carbon monoxide poisoning, methemoglobinemia) "High affinity" hemoglobins that will not give up their oxygen to the tissues
![Page 7: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/7.jpg)
Direct Physical ActionMajor problems are hemorrhage & ischemia
![Page 8: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/8.jpg)
INFECTIOUS DISEASE
Primary HerpesCandidiasis
Tuberculosis Actinomycosis
![Page 9: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/9.jpg)
PHYSICAL INJURY
Thermal Burn Traumatic ulcer
![Page 10: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/10.jpg)
CHEMICAL/DRUG INJURY
GingivalHyperplasia
Asprin Burn
![Page 11: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/11.jpg)
GENETIC DERANGEMENTS
Down's Syndrome
Ehlers-Danlos
Cancer
![Page 12: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/12.jpg)
NUTRITIONAL IMBALANCE
Diabetes
Scurvy
![Page 13: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/13.jpg)
Reversible Injury
• Mitochondrial oxidative phosphorylation is disrupted first Decreased ATP – Decreased Na/K ATPase gain of
intracellular Na cell swelling– Decreased ATP-dependent Ca pumps
increased cytoplasmic Ca concentration– Altered metabolism depletion of glycogen– Lactic acid accumulation decreased pH– Detachment of ribosomes from RER
decreased protein synthesis
• End result is cytoskeletal disruption with loss of microvilli, bleb formation, etc
![Page 14: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/14.jpg)
HYPOXIA - ISCHEMIA MODEL
Impaired function of the plasma membrane
ATP-dependentNa+ pump
Na+ influxCa++ influxK+ efflux
H2O influx
Cellular swellingMembrane blebs and loss of villiER swelling
![Page 15: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/15.jpg)
Irreversible Injury
• Mitochondrial swelling with formation of large amorphous densities in matrix
• Lysosomal membrane damage leakage of proteolytic enzymes into cytoplasm
• Mechanisms include:– Irreversible mitochondrial dysfunction
markedly decreased ATP– Severe impairment of cellular and organellar
membranes
![Page 16: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/16.jpg)
Downloaded from: StudentConsult (on 8 September 2010 02:58 PM) © 2005 Elsevier
![Page 17: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/17.jpg)
Cell Injury
• Membrane damage and loss of calcium homeostasis are most crucial
• Some models of cell death suggest that a massive influx of calcium “causes” cell death
• Too much cytoplasmic calcium:– Denatures proteins– Poisons mitochondria– Inhibits cellular enzymes
![Page 18: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/18.jpg)
![Page 19: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/19.jpg)
Clinical Correlation
• Injured membranes are leaky
• Enzymes and other proteins that escape through the leaky membranes make their way to the bloodstream, where they can be measured in the serum
![Page 20: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/20.jpg)
Reactive oxygen and nitrogen species ROS/RNSFree radical – each molecule or its fragment, which can exists independently And contains one or two unpaired electronsReactive oxygen species - species, which contain one or more oxygen atoms and are much more reactive than molecular oxygen
ROS/RNSFree radicals
superoxide radical
hydroperoxyl radical
hydroxyl radical
nitric oxide
hydrogen peroxide
![Page 21: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/21.jpg)
Free Radicals
• Free radicals have an unpaired electron in their outer orbit
• Free radicals cause chain reactions
• Generated by:– Absorption of radiant energy– Oxidation of endogenous constituents– Oxidation of exogenous compounds
![Page 22: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/22.jpg)
Examples of Free Radical Injury
• Chemical (e.g., CCl4, acetaminophen)
• Inflammation / Microbial killing
• Irradiation (e.g., UV rays skin cancer)
• Oxygen (e.g., exposure to very high oxygen tension on ventilator)
• Age-related changes
![Page 23: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/23.jpg)
Mechanism of Free Radical Injury
• Lipid peroxidation damage to cellular and organellar membranes
• Protein cross-linking and fragmentation due to oxidative modification of amino acids and proteins
• DNA damage due to reactions of free radicals with thymine
![Page 24: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/24.jpg)
Downloaded from: StudentConsult (on 8 September 2010 02:58 PM) © 2005 Elsevier
![Page 25: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/25.jpg)
IRREVERSIBLE CELL INJURY
Definition:Necrosis is local death of cells while the individual is a life
followed by morphological changes in the surrounding living tissue, (cell placed immediately in fixative are dead but not necrotic).
Causes of cell necrosis: most common causes of cell death are viruses, ischaemia, bacterial toxins, hypersensitivity, and
ionizing radiation.
Morphologic change in necrosis:The changes don’t appear in the affected cells by light microscopy
before 2-6 hours according to the type of the affected tissue.
NECROSIS
![Page 26: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/26.jpg)
Changes in the cytoplasm:
i. Swelling and granularity of the cytoplasm
ii. Loss of cellular membrane
iii. Fusion of cells
![Page 27: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/27.jpg)
Nuclear changes: Occur due to hydrolysis of
nucleoproteins:
i. Pyknosis i.e. the nucleus becomes shrunken condensed and deeply stained.
ii. Karyorrhexis: rupture of nuclear membrane with fragmentation of the nucleus.
iii. Karyolysis: the nucleus dissolves and disappears.
Finally the affected tissue changes to homogeneous eosinophilic mass with nuclear debris.
![Page 28: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/28.jpg)
TYPES OF NECROSIS
![Page 29: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/29.jpg)
The variable types of necrosis differ as regards causes, gross and microscopic pictures.
(1) Coagulative necrosis:It is mainly caused by sudden ischaemia e.g. infarction of
heart, kidney and spleen. The protein of the affected tissue becomes denaturated.
Grossly, it appears dry pale opaque. It is triangular ? subcapsular with the base towards the capsule of the affected organ. This is due to the fan like distribution of the supplying blood vessels. The infarct area is surrounded by narrow zone of inflammation and congestion.
Microscopically, the structural outline of the affected tissue is preserved but the cellular details are lost.
TYPES OF NECROSIS
![Page 30: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/30.jpg)
![Page 31: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/31.jpg)
(2) Liquifactive necrosis
The necrosed tissue undergoes rapid softening e.g. infarction of the nervous tissue which has abundant lysosomal enzymes. Also, this type of necrosis occurs in case of suppurative inflammation (Abscess) where liquefaction occurs under the effect of proteolytic enzymes of PNLs liquefaction of the amoebic abscess occurs due to the effect of strong proteolytic enzymes and hyaluronidase secreted by E. Histolytica.
Grossly: the affected tissue appears as homogenous amorphous substance. Microscopically: it appears as homogenous eosinophilic structure.
.
![Page 32: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/32.jpg)
The two lung abscesses seen here are examples of liquefactive necrosis in which there is a liquid center in an area of tissue injury. One abscess appears in the upper lobe and one in the lower lobe. Liquefactive necrosis is typical of organs in which the tissues have a lot of lipid (such as brain) or when there is an abscess with lots of acute inflammatory cells whose release of proteolytic enzymes destroys the surrounding tissues.
![Page 33: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/33.jpg)
Lung Abscess: Microscopic appearance (Liquefactive Necrosis)
![Page 34: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/34.jpg)
(3) Caseous necrosis:• It is characteristic of tuberculosis. The necrotic
tissue undergoes slow partial liquefaction forming yellow cheesy material.
• Microscopically, it shows amorphous granular eosinophilic material lacking the cell outlines.
• Unlike coagulative necrosis, the necrotic cells do not retain their cellular outlines, and do not disappear by lysis, as in liquifactive necrosi
• Grossly, the caseous material resembles clumpy cheese, hence the name caseous necrosis.
• The cause of necrosis in TB is hypersensitivity reaction caused by the tuberculoprotein content of the cell wall of Mycobacterium..
![Page 35: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/35.jpg)
Caseous necrosis with granulomatous inflammation
T.B LUNG : (Large Area Of Caseous Necrosis)
AREA ,YELLOW-WHITE AND CHESSY
![Page 36: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/36.jpg)
(4) Fat necrosis it is necrosis of adipose tissue including
two types:
a) Traumatic: caused by trauma to adipose tissue e.g. breast and subcutaneous tissue.
b) Enzymatic: which occurs in case of acute haemorrhagic pancreatitis.
Obstruction of the pancreatic duct leads to release of lipase which splits the fat cells of the omentum into fatty acid (combine with Ca giving chalky white calcification) and to glycerol which is absorbed in the circulation.
![Page 37: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/37.jpg)
![Page 38: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/38.jpg)
(5) Fibrinoid necrosis This is characterized by swelling, fragmentation, increased eosinophilia of collagen fibers and accumulation of mucopolysaccharides and fibrin due to vascular exudation of fibrinogen at the site of lesion, e.g.:
a) Collagen diseases (Rheumatic fever, Rheumatoid, Sclerodermia, Lupus erythematosus and Polyarteritis nodosa).
b) In the wall of blood vessels in malignant hypertension
![Page 39: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/39.jpg)
![Page 40: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/40.jpg)
(6) Zenker’s necrosis:
Of the rectus abdominus muscle and
diaphragm as a complication of :bacterial infection particularly typhoid
fever.
The striated muscles lose its striation, swell and fuse together in homogeneous structureless mass.
![Page 41: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/41.jpg)
Gangrene
• Gangrene is defined as the gradual destruction of living tissue, due to an obstruction in the supply of blood and oxygen to an area of the body (Pipkin and Janelli, 2000)
Obstruction of the blood supply to the bowel is almost followed by gangrene.
![Page 42: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/42.jpg)
(7) Gangrenous necrosis:
The tissue in this case have undergone ischaemic cell death and coagulative necrosis followed by liquifactive action of putrefactive organisms. When coagulative pattern is dominant the process is termed dry gangrene.
When the liquifactive action of the bacteria
is more pronounced it is called wet gangrene.
![Page 43: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/43.jpg)
“Dry Gangrene”
"wet gangrene in patient with Diabetes millitus”
![Page 44: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/44.jpg)
CLOSTRIDIAL GANGRENE
• (including "gas gangrene"), a dread complication of dirty, blood-deprived wounds. The clostridia digest tissue enzymatically and rapidly, often transforming it into a bubbly soup.
![Page 45: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/45.jpg)
FOURNIER'S GANGRENE
• Fournier's gangrene, bacterial gangrene of the scrotum (the dreaded "black sack disease")
![Page 46: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/46.jpg)
![Page 47: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/47.jpg)
ApoptosisDefinition:• It is programmed death of cells in living tissues. It is
an active process differing from necrosis by the following points:
• Occurs in both physiological and pathological conditions.
• Starts by nuclear changes in the form of chromatin condensation and fragmentation followed by cytoplasmic budding and then phagocytosis of the extruded apoptotic bodies.
• Plasma membrane are thought to remain intact during apoptosis until the last stage so does not initiate inflammatory reaction around it.
![Page 48: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/48.jpg)
![Page 49: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/49.jpg)
![Page 50: Cell injury, apotosis and necrosis(1)](https://reader033.fdocuments.net/reader033/viewer/2022051514/548775bdb47959050d8b5504/html5/thumbnails/50.jpg)
• PAGES , 13, 26-30
• DIFFERENCE BETWEEN AOPTOSIS AND NECROSIS, PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS, MECHANISM OF APOPTOSIS,
•ROBBIN’S BASIC PATHOLOGY, 7TH EDD, MAJOR