Introduction to Histology Cell Structures Dr. Rebecca A. Code Summer Scholars 2007.
Cell histology
-
Upload
helnamaria -
Category
Education
-
view
748 -
download
0
Transcript of Cell histology
![Page 1: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/1.jpg)
The Cell
Observed by Light and Electron Microscopy
![Page 2: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/2.jpg)
Overview of the Cellular Organelles
![Page 3: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/3.jpg)
Light Microscopy – Hematoxylin & Eosin (H&E) Stain
Basophilic (blue) Nuclei
Eosinophilic (red)
cytoplasm
![Page 4: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/4.jpg)
Colorized low mag TEM of a cell
Red = Mitos
Blue = Ribos
Green = RER
![Page 5: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/5.jpg)
Colorized TEM of a cell nucleus
Arrow = Nucleolus
Red = Mitos
Green = RER
![Page 6: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/6.jpg)
Nuclear Structures
![Page 7: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/7.jpg)
Low mag TEM of a nuclei
![Page 8: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/8.jpg)
Nuclear pores (arrows) Nuclear pores (en face)
![Page 9: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/9.jpg)
Colorized TEM of nuclear pores (blue) en face
![Page 10: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/10.jpg)
High mag TEM of a nucleolus
Pars fibrosa/granulosa (dense black) and
fibrillar center (arrow)
![Page 11: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/11.jpg)
TEM of interphase nuclei and a mitotic cell with condensed chromosomes (arrow)
![Page 12: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/12.jpg)
Cytoplasmic Structures
Ribosomes, RER and Glogi
![Page 13: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/13.jpg)
Ribosomes by light microscopy = basophilic cytoplasm
![Page 14: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/14.jpg)
TEMs of free ribosomes – 25 nm granules – in clusters called polysomes
![Page 15: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/15.jpg)
Colorized TEM of polysomes (yellow highlighted)
![Page 16: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/16.jpg)
Low mag TEM of rough endoplasmic reticulum (RER)
![Page 17: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/17.jpg)
Colorized higher mag TEM of RER (green)
![Page 18: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/18.jpg)
TEM of RER – arrows indicate cisternae
![Page 19: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/19.jpg)
Low mag TEM of RER and Golgi (Go)
![Page 20: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/20.jpg)
Colorized TEM of a Golgi system
![Page 21: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/21.jpg)
TEM of a Golgi system
![Page 22: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/22.jpg)
TEM of lysosomes (arrows) & a residual body
Residual Body
![Page 23: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/23.jpg)
Cytoplasmic inclusions by Light Microscopy
Basophilic granules (top picture)
Eosinophilic granules (bottom picture)
![Page 24: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/24.jpg)
Mitochondria
![Page 25: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/25.jpg)
Colorized TEM of Mitochrondria (red)
![Page 26: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/26.jpg)
TEMs of Mitochrondrial shapes
![Page 27: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/27.jpg)
TEM of a Mitochrondrion
Arrows = cristae
![Page 28: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/28.jpg)
The Cytoskeleton
![Page 29: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/29.jpg)
Light microscopy of mitotic spindles = Microtubules
![Page 30: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/30.jpg)
Colorized TEM of Cilia in cross section showing microtubules
![Page 31: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/31.jpg)
Immunofluorescence of Microtubules in cultured cells (green)
![Page 32: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/32.jpg)
TEM of Microfilaments (arrows)
![Page 33: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/33.jpg)
Immunofluorescence of microfilament stress fibers in cultured cells
![Page 34: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/34.jpg)
Cell Membrane
![Page 35: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/35.jpg)
High mag TEM of two cell membranes (unit membrane)
![Page 36: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/36.jpg)
Colorized TEM of two cell membranes (unit membrane)
![Page 37: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/37.jpg)
TEMs of membrane specializations
Top 4 = microvilli
Middle = Stereo cilia
Bottom = Cilia
![Page 38: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/38.jpg)
TEM of Cilia (C) with microtubular cores forming from basal
bodies (BB)
![Page 39: Cell histology](https://reader033.fdocuments.net/reader033/viewer/2022052602/559a288b1a28abda228b4756/html5/thumbnails/39.jpg)
High mag TEM of Cilia with
microtubular cores (Fil) with the basal body (FiC) at top of
image