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It’s a dynamic time to be working in life sciences.
We know that every day, you keep pressing toward scientific breakthroughs in spite of
increased uncertainty, shifting technology, and decreased funding.
Your work is already complex enough. But now more than ever, being at the forefront ofdiscovery means navigating through change.
And we are there to support you at every turn.
That’s why we are making a few adjustments on our end to help simplify things on yours.
We’re assessing how we do things—improving where we need it, and continuing to do what
already works.
We’ve made our website more intuitive and straightforward so you can easily determinewhat products you need and find them all in one place.
We are creating new ways to offer products that are priced to meet your budget but don’tcompromise quality.
We’re ensuring that our people are highly skilled, readily available, and understand the manyfacets of your research, so you get answers quickly—the first time, every time.
We are making sure our world-class support team members operate more like trustedcolleagues—and less like “representatives”—to walk you through solutions step by step,with simple answers and calming reassurance.
Most importantly, we are keeping our focus on makinginnovation brilliantly simple to improve life in your lab.
So you can keep driving toward discoveries that
improve life for all of us.
We are here to support you at every turn.Let us know how we can do that at facebook.com/lifetechnologies
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Cell biology resources
©2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.
Cellbiologyproduc
ts
Cell culture and cells (Chapter 2) Cell analysis (Chapter 3)
Sample
preparation
• Recovery™ Freezing Medium, page 40• Gibco® RPMI Media 1640, page 41• Gibco® Minimal Essential Medium (MEM), page 41• GlutaMAX™ media, page 41• Opti-MEM® reduced-serum medium, page 41
• Dynabeads® cell isolation technology, page 76• Dynabeads® CD3/CD28 technology, page 79
Key
technologies
• Expi293™ Expression System, page 42• FreeStyle™ MAX CHO Expression system, page 42• AlgiMatrix® 3D cell culture system, page 49• Geltrex® LDEV-free matrix products, page 49• AmnioMAX™ media, page 51• MarrowMAX™ bone marrow medium, page 51• PB-MAX™ Karyotyping Medium, page 51• KaryoMAX® medium, page 51• TrypLE™ reagent, page 52
• Click-iT® EdU cell proliferation assay, page 82• Premo™ FUCCI Cell Cycle Sensor, page 83• CellROX™ Deep Red Reagent, page 86• CellLight® fluorescent protein constructs, page 87• Qtracker® Cell Labeling, page 95• FluoroMyelin™ Green fluorescent myelin stain, page 95• Molecular Probes® antibodies for multicolor flow
cytometry, page 102• Qdot® nanocrystals for flow cytometry, page 105• Zenon® antibody labeling, page 107• APEX® antibody labeling, page 107
Essentials
• Classical Gibco® media: DMEM, RPMI, and MEM,page 41
• Advanced Gibco® media: GlutaMAX™, OptiMEM™,page 41
• Gibco® FBS, page 43• Geneticin® selection antibiotic, page 46• Puromycin seletion antibiotic, page 46• Zeocin™ selection antibiotic, page 46• Gibco® growth factors, page 47• B-27® Supplement, serum substitute for neural cell
culture, page 72• Neurobasal® Media, basal media, page 48• KnockOut™ Serum Replacement, for pluripotent stem
cells, page 60
• DynaMag™ magnets, page 80• HulaMixer® Sample Mixer, page 80• LIVE/DEAD® Viability/Cytotoxicity Kits, page 81• CellEvent® Caspase-3/7 Green Detection Reagent,
page 85• pHrodo™ indicators, page 87• Alexa Fluor® phalloidins, page 90• MitoTracker® dyes, page 91• JC-1 dye, page 91• LysoTracker® dyes, page 92• BODIPY® FL C
5-ceramide, page 93
• TO-PRO®-3, page 94• Calcein, AM, page 95• CellTracker™ dyes, page 95• Fluorescent dextrans, page 95• Alexa Fluor® conjugated secondary antibodies and
streptavidin, page 106
Detection
instruments
• Countess® Automated Cell Counter, page 53• Neon® Transfection System, page 55• Tali® Image-Based Cytometer, page 55• FLoid® Cell Imaging Station, page 55• Attune® Acoustic Focusing Cytometer, page 55
• Tali® Image-Based Cytometer, page 99• FLoid® Cell Imaging Station, page 101• Attune® Acoustic Focusing Cytometer, page 100
2013 Life Technologies
Cell biology product and resource guideComprising trusted and familiar Gibco®, Invitrogen™, Molecular Probes®, and Novex® products, the Life Technologies cell biology
portfolio offers published, high-performance, scalable solutions across the breadth of cell biology applications.
The book begins with overviews of five prominent cell biology research areas: neurobiology, immunology, cancer, stem cell biology,
and plant cell biology. Using published data, we’ve also constructed pathway maps for some of these research areas that you may
consult as you plan your research.
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Cell biology resources
Protein analysis (Chapter 4)
• Dynabeads® immunoprecipitation, page 129• Novex® organelle isolation kits, page 132• Novex® protein A/G purification, page 130• Novex® cell lysis reagents, page 133
Protein expression• Champion® pET Expression System, page 119• PichiaPink® Yeast Expression System, page 118• GeneArt® Algae Engineering Kit, page 120• GeneArt® Gene Synthesis, page 121• Lipofectamine® LTX Transfection Reagent, page 123• Lipofectamine® RNAiMAX Reagent, page 123• Lipofectamine® 2000 Transfection Reagent, page 123• Silencer ® Select siRNA, page 126• Stealth Select siRNA and Silencer ® siRNA, page 126• mir Vana™ miRNA Mimics and Inhibitors, page 128
Protein analysis• Bolt™ and NuPAGE® protein gels, page 136• Novex® Sharp and SeeBlue® Plus 2 Pre-Stained Standard,
page 141 • SYPRO® Ruby Protein Gel Stain, page 142• Novex® ABfinity™ antibodies, page 147• Novex® multiplex immunoassays for Luminex® platforms, page 153• Novex® magnetic multiplex immunoassay for Luminex®, page 154
Protein expression• Ambion® Pre-miR™ Precursors and Anti-miR™ Inhibitors,
page 128• Lipofectamine® reagents, page 123
Protein analysis• Novex® pour-your-own gel essentials, page 136• Novex® Tris-glycine gels, page 136• SeeBlue® Pre-Stained Standard, page 141• SimplyBlue® SafeStain, page 142• Novex® Antibody Pair kits, page 150• Novex® ELISA kits, page 150
• Qubit® Fluorometer, page 134• Neon® Transfection System, page 124• POROS® analytical chromatography, page 131• ZOOM® IEF Fractionator, page 132
• XCell SureLock® Protein Electrophoresis Cells, page 139• iBlot® 7-Minute Blotting System, page 143• BenchPro® 4100 Western Processing System, page 144• MAGPIX® System, page 155
Chapter 1 contains tools you may find useful to choose products and interpret your data.
The rest of the guide is summarized in the chart below—an organized snapshot of the most popular of our reliable solutions,backed by nearly 3 million publications. For complete product information, refer to the chapter/page numbers noted.
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1 Cell biology resources
2Cell culture and cells
3
Cell analysis
4 Protein analysis
The products listed in this document have the following product uses.
Product type Product Use
Research use For Research Use Only. Not for use in diagnostic procedures.
Class I - IVD In vitro Diagnostic Use
CTS brand research use For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into human or animals.
OR
For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research. Caution: Not intended for direct administrationinto humans or animals.
Class II medical device For human ex vivo tissue and cell culture processing applications: CAUTION: When used as a medical device, Federal Law restricts this device to saleby or on the order of a physician.
Unless otherwise indicated, the products included in this catalog are For Research Use Only. Not for use in diagnostic procedures.
0Cell biology research and pathways
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Cell biology research and pathways
Cell biology research and pathways
Research areas—This section provides an overview for investigators in aspecific discipline to see the broad menu of products, services, and toolsLife Technologies offers for their research and tools.
Neurobiology research 8Immunology research 14
Cancer research 16
Stem cell research 18
Plant cell biology research 20
Cellular signaling pathways—Refer to these publication-supportedrepresentations of several important signaling pathways.
AKT pathway 22
MAPK pathway 24
JAK/STAT pathway 26
Neurodegeneration 28
Custom services—Take advantage of these terrific serviceofferings to customize your tools.
Gibco® custom media services 30
Custom Novex® multiplex assays for Luminex® technology 32
Custom biology services 33
Website resources—All of these resources are available at a click, includingresources for learning techniques, finding the right cell line by application,downloading protocols, stain and antibody selection guides, and mobile appsfor the scientist on the go.
Gibco® Cell Culture Basics 34
Cell Line Database 34
Protocol Database 35
The Molecular Probes® Handbook online 35
Fluorescence SpectraViewer 36
Mobile applications and widgets 36
Virtual Cell Staining Tool 36
Antibody and immunoassay selection guides 37
Customer and technical support 37
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8 ©2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.
Cell biology research and pathways
Neurobiologyresearch
Neurobiology researchLife Technologies offers a range of cells and cell culture tools as well as cellular analysis assays and
reagents for research on neural primary and stem cells. Our products encompass all major neural cell
types and support a multitude of applications from basic research to therapeutic discovery. Select the right
tools for your neuroscience research at lifetechnologies.com/neuro.
Cells and culture tools for neurobiologyNeural primary and stem cells
Gibco® offers a wide array of highly pure, ready-to-use primary and stem cells for neural research that
have high viability and superior performance. For more details on cells available, visit lifetechnologies.com/
neuroculture.
Neural primary cells:
• Rat cortex and hippocampus neuronal cells
• Rat primary cortical astrocytes
• Human astrocytes
Stem cells:
• Human neural stem cells
• Rat fetal neural stem cells
• Rat glial precursor cells
• Human dopaminergic precursor cells (available as custom order only)
Media and supplements
Selecting the right media system for your neural cell culture is important to ensure experimental consis-
tency, reproducibility, and relevancy. For over twenty years, neuroscience researchers have relied on Gibco®
products to ensure confidence in results. Cited in over 11,000 publications spanning more than 50 cell types
and an expansive list of disease models, Gibco® neural cell culture products deliver the versatility required
from media systems today.
Choose from supplements optimized to support a variety of applications, basal media designed for key cell
types, and quality primary and stem cells. To learn more, visit lifetechnologies.com/neuroculture.
To build the optimal media system for your neural cell culture needs, explore our product offering by cell
type, or browse our products designed for specific customer applications like electrophysiology or insulin
studies, in the tables on pages 9 and 10.
Primary rat hippo-campus neurons
Human neural stemcells
http://www.lifetechnologies.com/cbcat/neurohttp://www.lifetechnologies.com/neuroculturehttp://www.lifetechnologies.com/neuroculturehttp://www.lifetechnologies.com/neuroculturehttp://www.lifetechnologies.com/neuroculturehttp://www.lifetechnologies.com/neuroculturehttp://www.lifetechnologies.com/neuroculturehttp://www.lifetechnologies.com/cbcat/neuro
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Cell biology research and pathways
Tools for primary neurons.
Supplement Application Basal media Optimized cell type Cells Cell source
P r i m a r y n e u r o n s
B-27® Serum FreeSupplement
Growth andmaintenance ofneurons
Neurobasal® Medium
Optimized forprenatal and fetalneurons
Primary rat cortexneurons
Isolated from day-18Fisher 344 ratembryos
Neurobasal®-AMedium
Optimized forpostnatal and adultbrain neurons
Primary rathippocampusneurons
Isolated from day-18Fisher 344 ratembryos
DMEM media Growth of aspectrum ofmammalian celllines
G l i a l
c e l l s
G-5 Supplement Growth of glial celllines
A s t r o c y t e s
G-5 Supplement Astrocyte Medium Growth of primaryand serial tumorlines of astrocyticphenotype
Rat primary corticalastrocytes
Isolated fromcortices of Sprague-Dawley rats atembryonic day 18
(E19)
Growth of primaryhuman and ratastrocytes
Human astrocytes Normal human cellsderived from humanbrain tissue
Tools for neural stem cells and progenitor cells.
Supplement Application Basal media Optimized cell type Cells Cell source
N e u r a l s t e m c
e l l s
B-27® Supplement,minus Vitamin A
Proliferation of stemcells
StemPro® NSC SFM Human neural stemcells
Human neural stemcells
Derived from H9(WA09) humanembryonic stemcells
B-27®
Supplement,XenoFree Differentiation ofstem cells KnockOut™ DMEM/F-12 Medium Neural stem cells Rat fetal neuralstem cells Isolated from thecortex of Sprague-Dawley E14 rats
N-2 Supplement Serum substitute forembryonic neuralcells
StemPro® NeuralSupplement
Growth and expan-sion of human andrat neural stemcells (NSCs)
P r o g
e n i t o r c e l l s N-2 Supplement Serum substitute
for CNS progenitorcells
KnockOut™ DMEM/F-12 Medium
Neural stem cells Rat glial precursorcells (rGPCs)
Isolated fromnewborn Sprague-Dawley rat cortex
StemPro® Neural
Supplement
Growth and expan-
sion of progenitorcells
Human dopami-
nergic precursorcells*
Derived from H9
human embryonicstem cells (ESCs)
* Available as custom order.
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Neurobiologyresearch
Tools optimized for specialty applications.
Supplement Application
B-27® Supplement, minus AO (antioxidants)Studies of oxidative stress/damage,apoptosis, or where free radical damage toneurons occurs
B-27®
Supplement, minus insulinStudies of insulin secretion or insulinreceptors
B-27® Electrophysiology Kit Electrophysiology studies
B-27® Supplement, customGrowth and expansion of retinal ganglioncells
Basal media Application Optimized cell type
Neurobasal® Medium without phenol red Receptor studies, downstream purificationstudies, or other processes where the pres-ence of phenol red is undesired
Prenatal and fetal neurons
Neurobasal®-A Medium without phenol redOptimized for postnatal and adult brainneurons
Hibernate®-A Medium Manipulation of neurons at ambient CO2 for at
least 48 hr while retaining viability
Optimized for postnatal neurons
Hibernate®-E Medium Optimized for embryonic neurons
Serum-free supplements
• B-27® Supplements—optimized serum substitute for neural cell culture available exclusively from Life
Technologies; learn more at lifetechnologies.com/b27
• N-2 Supplement—serum substitute for embryonic neural and central nervous system progenitor cells
• G-5 Supplement—chemically defined, serum-free supplement for growth of glial cell lines
• StemPro® Neural Supplement—serum-free supplement formulated for the growth and expansion of human
and rat NSCs and progenitor cells
• Gibco® sera—deliver consistent cell growth over time and passages; learn more at lifetechnologies.com/
cellculture
Supplement comparison.
Standard for mammalian cellsIndustry standard for neuronal cellculture
Optimized for neuronal stem cells
Product Gibco® sera B-27® Serum Free SupplementStemPro® Neural Supplement &B-27® Supplement, XenoFree
Lot-to-lot consistency Highest grade only Yes Yes
cGMP manufacturing Yes Yes Yes
Performance testing Non-neural cell line proliferation Primary neuron cell survivalProliferation and differentiation ofhuman NSCs
Compatible cell types Neural and non-neural cell linesHuman and animal primary neuronsand glia
Human and animal NSCs
Formulations offered Qualified and certified Minus antioxidants, minus insulin, orelectrophysiology Minus vitamin A and XenoFree
Learn more lifetechnologies.com/cellculture lifetechnologies.com/b27 lifetechnologies.com/NSC
Cell biology research and pathways
http://www.lifetechnologies.com/b27http://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/b27http://www.lifetechnologies.com/NSChttp://www.lifetechnologies.com/NSChttp://www.lifetechnologies.com/b27http://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/b27
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Basal media
• Neurobasal® media—support growth of neurons when combined with B-27®,
N-2, or G-5 supplements and designed to inhibit glial proliferation; learn more at
lifetechnologies.com/neurobasal
• KnockOut™ DMEM/F-12 Medium—optimized for growth of undifferentiated stem
cells; supports growth of NSCs when used with StemPro® Neural Supplement
• StemPro® NSC SFM—serum-free medium formulated for growth and expansionof human NSCs derived from either ESCs or fetal tissue; kit includes KnockOut™
DMEM/F-12 medium, StemPro® Neural Supplement, and growth factors
• Gibco® DMEM—Classic formulation used to culture glial cells (astrocytes and
oligodendrocytes)
Cell substrates
• Natural mouse laminin—important for cell attachment, spreading, and neurite
outgrowth
• Fibronectin—modulates cell adhesion, growth, migration, and differentiation as a
component of the extracellular matrix
• CELLstart™ CTS™ Substrate—fully defined, xeno-free substrate for stem cell
growth and expansion; maintains multipotency of human NSCs in StemPro
®
NSC SFM
Enzymatic passaging
• StemPro® Accutase® Cell Dissociation Reagent—designed for gentle and efficient
detachment of neural stem cells, progenitor cells (including glial), and primary
neural cells
Cryopreservation
• Synth-a-Freeze® Cryopreservation Medium—a defined liquid cryopreservation
medium for human keratinocytes, ESCs, NSCs, and mesenchymal stem cells
• Hibernate® media—ideal for manipulation of neurons at ambient CO2 and as pres-
ervation media for viable brain tissue for up to a month at 4°C
Cell biology research and pathways
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Neurobiologyresearch
Cellular analysis assays and reagents for neurobiologyCell viability assays
We have a range of cell viability assays to assess the health of your neural cells.
View more information and compare cell viability assays at lifetechnologies.com/cellhealthneuro.
Types of cell viability assays.
Fast resazurin-basedassays
Quantitative measurementof DNA content
Quantitative measurementof neurite outgrowth
Simultaneous quantifica-tion of live and dead cells
Product PrestoBlue® Cell ViabilityReagent
CyQUANT® Cell Prolifera-tion Assay
Neurite Outgrowth StainingKit
LIVE/DEAD® cell viabilityassays
Assay type Single parameter Single parameter Multiplexed Multiplexed
Mechanism of action Metabolic activity DNA quantification Esterase activity,membrane integrity
Esterase activity,membrane integrity
Format Live Live Live or endpoint Endpoint
Incubation time >10 min 30–60 min 30 min 15–45 min
Readout Absorbance or fluores-
cence
Fluorescence Fluorescence Fluorescence
Instrument compat-ibility
Microplate reader Microplate reader, micro-scopy
Microplate reader, micro-scopy
Flow cytometry, microplatereader, microscopy
For more info, visit: lifetechnologies.com/prestoblue
lifetechnologies.com/cyquantdirect
lifetechnologies.com/neuriteoutgrowth
lifetechnologies.com/livedead
Fluorescent probes for neuronal physiology
Choose from a diverse selection of fluorescent ion indicators and probes for calcium, sodium, zinc, potassium,
magnesium, pH, membrane potential, and synaptic function from the industry leader in fluorescent technology.
• FluxOR™ Potassium Ion Channel Assay—an optically based, homogeneous assay for high-throughput
screening measurements of potassium ion channel and transporter activities; learn more atlifetechnologies.com/fluxor
• Fluo-4 Direct™ Calcium Assay Kit—advanced formulation that suppresses background fluorescence
generated from media with negligible impact on the cellular fluorescence, allowing the assay to be
run in a simple “addition-only” format in the presence of serum-containing media; learn more at
lifetechnologies.com/fluo4direct
• Voltage sensor probes—these probes use a fluorescence resonance energy transfer-based voltage-sensing
assay technology to measure changes in cell membrane potential; learn more at lifetechnologies.com/vsp
• FM® probes for synaptic function—these probes are ideal for investigating the mechanisms of synaptic
vesicle activity; learn more at lifetechnologies.com/fmprobes
Probes for neuronal tracing and anatomy
Choose from an extensive range of fluorescent and biotinylated tracers for retrograde and anterograde
tracing, fluorescent histological stains, and ligands for receptors, ion channels, and ion carriers.
Visit lifetechnologies.com/neuroprobes for a full list of probes for neuronal tracing and anatomy.
Cell biology research and pathways
http://www.lifetechnologies.com/cellhealthneurohttp://www.lifetechnologies.com/prestobluehttp://www.lifetechnologies.com/prestobluehttp://www.lifetechnologies.com/cyquantdirecthttp://www.lifetechnologies.com/cyquantdirecthttp://www.lifetechnologies.com/neuriteoutgrowthhttp://www.lifetechnologies.com/neuriteoutgrowthhttp://www.lifetechnologies.com/livedeadhttp://www.lifetechnologies.com/livedeadhttp://www.lifetechnologies.com/fluxorhttp://www.lifetechnologies.com/fluo4directhttp://www.lifetechnologies.com/vsphttp://www.lifetechnologies.com/fmprobeshttp://www.lifetechnologies.com/neuroprobeshttp://www.lifetechnologies.com/neuroprobeshttp://www.lifetechnologies.com/fmprobeshttp://www.lifetechnologies.com/vsphttp://www.lifetechnologies.com/fluo4directhttp://www.lifetechnologies.com/fluxorhttp://www.lifetechnologies.com/livedeadhttp://www.lifetechnologies.com/livedeadhttp://www.lifetechnologies.com/neuriteoutgrowthhttp://www.lifetechnologies.com/neuriteoutgrowthhttp://www.lifetechnologies.com/cyquantdirecthttp://www.lifetechnologies.com/cyquantdirecthttp://www.lifetechnologies.com/prestobluehttp://www.lifetechnologies.com/prestobluehttp://www.lifetechnologies.com/cellhealthneuro
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Detection of neuro-related proteins
Tools for neuro-related protein detection.
Novex® product Qualitative Industry standard Multiplexable
Western blotting ELISA Multiplex assay using Luminex® platform
Analytes per well One One Up to 50 (proteins)Hands-on time 2–3 days 3–5 hours 4–5 hours
Dynamic range Low Medium High
For more info visit Western products:lifetechnologies.com/westerndetectionAntibodies:lifetechnologies.com/abfinity lifetechnologies.com/antibodies
lifetechnologies.com/neuroelisa lifetechnologies.com/luminex
Cellular expression analysis
BacMam technology
BacMam technology allows simple, safe, efficient delivery and expression of genes of interest in mammaliancells, including neurons.
For more information about BacMam technology, visit lifetechnologies.com/bacmam.
Cellular and pathway analysis
Target-based assays
Select from our broad range of validated cell-based assays for key neural targets, including G-protein–
coupled receptors, kinases, and ion channels.
Visit lifetechnologies.com/discoveryassays.
Custom assay development
Let us create and validate a custom assay that meets your defined requirements.Visit lifetechnologies.com/custom.
Assay development and screeening
Use BacMam stocks Transduce mammalian cells Configure assays Perform screen or assay
BacMam workflow. Just add the BacMam reagent to your cells for 2–4 hours, treat with an enhancer (recommended for certain cell types including neurons), wash,incubate overnight, and perform the assay.
Cell biology research and pathways
http://www.lifetechnologies.com/westerndetectionhttp://www.lifetechnologies.com/abfinityhttp://www.lifetechnologies.com/antibodieshttp://www.lifetechnologies.com/neuroelisahttp://www.lifetechnologies.com/luminexhttp://www.lifetechnologies.com/bacmamhttp://www.lifetechnologies.com/discoveryassayshttp://www.lifetechnologies.com/customhttp://www.lifetechnologies.com/customhttp://www.lifetechnologies.com/discoveryassayshttp://www.lifetechnologies.com/bacmamhttp://www.lifetechnologies.com/luminexhttp://www.lifetechnologies.com/neuroelisahttp://www.lifetechnologies.com/antibodieshttp://www.lifetechnologies.com/abfinityhttp://www.lifetechnologies.com/westerndetection
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Immunologyresearch
Immunology researchAt Life Technologies, we understand the importance of using the highest-quality and most sensitive reagents
to dissect the intricacies associated with immunology research. Our extensive portfolio of products can be used
across all stages of T and B cell development and activation, from isolation to stimulation and from expansion
to analysis.
• Isolate: Dynal® magnetic cell separation technologies yield pure,
uncontaminated cells without columns.
• Stimulate: Our validated reagents allow better control using state-of-the-art
expansion technology and Gibco® cytokines with consistently high bioactivity.
Ambion® siRNA technology is ideal to knock down key genes and proteins
involved in immunology processes.
• Analyze: We offer products for flow cytometry, imaging microscopy, western
blot analysis, and ELISA as well as Novex® multiplex assays for protein
quantification using Luminex® xMAP® technology.
Check out our immunology-related signaling pathways on pages 15 and 22-29.
These include:
• The inflammatory response
• The AKT pathway
• The MAPK pathway
• The JAK/STAT pathway
• Neurodegeneration
Find a comprehensive selection of our immunology products at lifetechnologies.com/immunology.
Below is a quick guide to the sections where you can learn more about immunology products and solutions from Life Technologies.
Reagents Dynabeads® magnetic cell separationproducts for:• Human cells• Mouse cells• Other cell typesIsolation of your own antibody cellsCD4+ cell counting kitsPrimary antibodies for:• Human immunology• Mouse immunology• Rat immunology
Specialty media for immunology research:• Gibco® OpTmizer™ Cell Expansion
Serum-Free Medium• Gibco® Serum-Free AIM V® Medium• Gibco® Macrophage Serum-Free Medium• Serum-free medium for hematopoietic
stem cell culture• Cytogenetic mediaRecombinant growth factors and cytokines
Primary antibodies for:• Human immunology• Mouse immunology• Rat immunologyPhosphorylation site–specific primaryantibodiesSecondary detection reagentsAntibody pairs
Assays and kits Positive isolationNegative isolationDepletion
Novex® multiplex assays for the Luminex® platformELISA kitsCell viability kitsCell proliferation kits
Technologies Dynabeads® technology for mouse andhuman T cell activation and expansionsiRNA
Click chemistryLuminex® xMAP® technologyCellLight® reagents (BacMam technology)
ABfinity™ recombinant monoclonal antibodytechnology
Instrumentation Countess® Automated Cell Counter Neon® Transfection System Attune® Acoustic Focusing CytometerFLoid® Cell Imaging StationTali® Image-Based CytometerMAGPIX® SystemiBlot® Western Transfer System
Accessories Magnets and mixers Flow cytometry controls and standardsImaging standards and calibration kitsProtein labeling kitsChemiluminescence detection systems
Isolate Stimulate Analyze
Cell biology research and pathways
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Fibroblastliver TNF-α
IL-1β
TGF-β
PDGFIL-6
M-CSF, TNF-α, GM-CSF,
G-CSF, IL-1, IL-6, IL-11,IL-12
IL-8,
TNF-α
IL-3, IL-4, IL-10,
IL-13, IFN-γ, TNF-β
TGF-β, IL-2,IL-4,IL-5, IL-13,
IFN-γ
IL-6IL-2
IL-12, IL-15
IL-3, IL-4, IL-5
IL-3, IL-4,IL-10
IL-3, IL-6,IL-7, GM-CSF
IL-17A, IL-17F,IL-22, IL-6,
GM-CSF
IL-2, IFN-γ, IL-4
IL-23
TNF, IL-1β
IL-18
IL-1, IL-6, IL-8, IL-10,IL-12, IL-15, IFN-α, IFN-β,
TNF-α, MCP-1, MIP-1α
IL-10, IL-14,
IL-4, IL-6
PECAM-1, VCAM-1,ICAM-1, P-selectin,
E-selectin
LFA-1, VLA-4,Mac-1
Acute phaseproteins
IgE
IgM/G/A
IFN-γ
Th9
Neutrophil elastaseRespiratory burst
Reactive oxygen species
H i s t a m
i n e
Activated B cell
Endothelialcell
NK cell
Th1/Th2
CTL
ActivatedT cell
Th17/22
Neutrophil
Resting T cell
Mast cell
Plasma cell
Eosinophil
Resting B cell
Bacterial products(LPS, peptidoglycans, etc.)
Macrophage
Humoral immunity
Allergy anaphylaxisViral infection,
tumor surveillance
Fibrosis Autoimmunity
Tissue repair,protozoan immunity
Cardiovascularpathology
Innate immunity
Viral infection,tumor surveillance
Allergy anaphylaxis
Hematopoiesis
Fever
Inflammation
Lungepithelium
IL-2
Eotaxin
The inflammatory response in immunology.
Cell biology research and pathways
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Cancerresearch
Cancer researchAt Life Technologies, we are dedicated to advancing cancer research and the
pursuit of a world without cancer—a goal we share with you, the research
and medical community. We are here to provide you with the instruments,
reagents, and technologies for cancer genomic and cancer cell analysis
applications to help translate your ideas from basic cancer research to
future clinical applications.
Translating genomic discoveries from the bench to the bedside and beyondThe key to fighting cancer through better therapeutics depends on a better understanding of the basic biology of this disease.
A major challenge in cancer research is unraveling the causes of cancer on a molecular level. Because most of the genomic
mutations or alterations in cancer impact the way that cells communicate with other cells, a solid understanding of both
genomic and cellular mechanisms is needed to accelerate cancer research.
At Life Technologies, we are committed to providing the widest technology selection with the highest quality at every budget to
help address challenges in cancer genomics and cancer cellular analysis applications.
Overview of key cancer cellular analysis and genomics applications.
Cell Viability & Proliferation
Cancer CellImaging
CancerStem Cells
EpigeneticsCancer
Immunotherapy
Cell Signaling& Pathway
Analysis
Cancer CellGrowth &
Manipulation
TargetedSequencing
& Gene Panels
Whole Genome& Whole Exome
Sequencing
GenotypingAssays-Somatic
Germline
WholeTranscriptome
Gene Expression-Focused panels
& assays
Gene Expression by Sequencing
Cancer Cellular Analysis Applications Cancer Genomics Applications
Observe, follow, and probe deeper into cellular networks Identify changes in the genomes of tumorsthat “drive” cancer progression
Cell biology research and pathways
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Cancer cellular analysis and genomic applicationsCancer cellular analysis involves understanding complex defects in communication between cells that alter
normal programs of proliferation, transcription, growth, migration, differentiation, and death.
Further advances in understanding altered cancer pathways and their interconnections may accelerate the
development of targeted molecular therapies allowing improvements in cancer diagnosis and treatment.
The right mix of tools and technologies are needed to observe, follow, and probe deeper into cancer cellular networks. With exper-tise that spans from cell culture, RNAi, and gene expression analysis to innovative products for rapid protein detection and cellular
imaging, we have affordable, high-quality solutions designed to help you reach your cancer research goals.
Learn more about the tools we offer for these applications below.
Cell viability, proliferation, and functionEvaluate the hallmarks of cancer withMolecular Probes® assays validated onmultiple instrument platforms, includingmicroscopes, flow cytometers, micro-plate readers, and high-content screeninginstruments. To learn more, visit lifetech-nologies.com/cellviability.
Cellular imagingVisualize intracellular changes in cancercells right on your benchtop using Molec-ular Probes® assays and the FLoid® CellImaging Station. To learn more, visitlifetechnologies.com/floid.
Image cell cycle progression in live cellswith the Premo™ FUCCI Cell Cycle Sensor.To learn more, visit lifetechnologies.com/premochameleon.
Cell signaling pathways
Analyze how cancer cell signaling path-ways are disrupted in cancer by accuratequantitation of intracellular and extracel-lular proteins using protein analysis toolsdesigned for single or multiplex cancerbiomarker analysis. To learn more, visitlifetechnologies.com/immunoassay andlifetechnologies.com/abfinity.
Cell growth and manipulation• Foster the right cells and culture
environments to decipher the cancercell signaling pathways influencedby altered genes. To learn more, visit
lifetechnologies.com/cellculture.• Identify genes and processes regulated
by your gene or pathway of interestthrough the power of RNAi. To learnmore, visit lifetechnologies.com/rnai.
• Use targeted editing of the genome toprecisely evaluate cancer phenotypesthrough target validation and cell-line optimization. To learn more, visitlifetechnologies.com/geneart.
Rare cell event analysisIdentify, track, and analyze cancer cellsignaling events in individual cancer cellsusing flow cytometry to study cell prolifera-tion, the cell cycle, apoptosis, and immu-nophenotyping and to detect rare cellularevents such as the presence of circulatingendothelial cells. To learn more, visitlifetechnologies.com/attune.
Cancer stem cellsIdentify quiescent cancer stem cells anddetermine their role in metastasis andtherapeutic resistance. To learn more,visit lifetechnologies.com/cancerstem-cells.
Cancer immunotherapyEfficiently isolate, activate, and culturevarious types of immune cells for yourcancer immunotherapy research needs.To learn more, visit lifetechnologies.com/
immunotherapyresearch.
Drug discovery and developmentDrive efficiency in drug assay development,high-throughput screening, and metabolicand safety assessment with the support,expertise, and customization to meet yourunique cancer research needs. To learn more,visit lifetechnologies.com/drugdiscovery.
Genotype-to-phenotype researchDetermine normal and cancer-related func-tions of genes, and the implication of varia-tion within those genes, to reach a better
understanding of cancer. To learn more, visitlifetechnologies.com/cancergenotype.
Cancer epigeneticsThe initiation and progression of cancer,traditionally seen as a genetic disease, isnow realized to involve epigenetic abnor-malities along with genetic alterations.Recent advancements in the rapidlyevolving field of cancer epigenetics haveshown that disruption of various epigen-etic mechanisms, including noncoding
RNAs such as microRNAs, DNA methyl-ation, histone modifications, and nucleo-some positioning, are central to switchingnormal cells to malignant cells.
Life Technologies has developed powerfulepigenetic research technologies span-ning renewable antibody development,
sample preparation, sequencing, quantita-tive PCR, and functional analysis assays.To learn more, visit lifetechnologies.com/cancerepigenetics.
Renewable epigenetic antibodies
Epigenetic regulatory proteins are involvedin a wide variety of chronic diseases,including cancer, and are the target of anexplosive new field of drug discovery. Theability to properly study most epigeneticchanges, which determine the genes thatare turned off and on, are determined bythe effectiveness of one critical tool—anti-
bodies. Life Technologies has partneredwith the Structural Genomics Consortiumto develop high-quality recombinant anti-bodies to read, write, and erase epigeneticcode. To learn more, visit lifetechnologies .com/epiantibodies.
Noncoding RNAs that regulate cancer
Discovering how noncoding RNAs regu-late gene expression will lead to newapproaches to understanding cancer.At Life Technologies, we are devel-oping sensitive methods to uncover new
RNAs, solutions to profile and validatemicroRNAs, and functional analysis toolsto help you define the most importantnoncoding RNAs in cancer. To learn more,visit lifetechnologies.com/cancerrna.
Cancer genomicsWe are committed to acceleratingcancer research with highly accurateand sensitive sequencing and real-timePCR systems to quickly find the cancermutations that matter. To learn more, visitlifetechnologies.com/cancerbiomarkers.
Cell biology research and pathways
http://www.lifetechnologies.com/cellviabilityhttp://www.lifetechnologies.com/cellviabilityhttp://www.lifetechnologies.com/floidhttp://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Cell-Viability-and-Regulation/Cell-Cycle/Live-Cell-Imaging-of-Cell-Cycle-and-Division.htmlhttp://www.lifetechnologies.com/premochameleonhttp://www.lifetechnologies.com/premochameleonhttp://www.lifetechnologies.com/immunoassayhttp://www.lifetechnologies.com/immunoassayhttp://www.lifetechnologies.com/abfinityhttp://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/rnaihttp://www.lifetechnologies.com/genearthttp://www.lifetechnologies.com/attunehttp://www.lifetechnologies.com/cancerstemcellshttp://www.lifetechnologies.com/cancerstemcellshttp://www.lifetechnologies.com/immunotherapyresearchhttp://www.lifetechnologies.com/immunotherapyresearchhttp://www.lifetechnologies.com/drugdiscoveryhttp://www.lifetechnologies.com/drugdiscoveryhttp://www.lifetechnologies.com/cancergenotypehttp://www.lifetechnologies.com/cancerepigeneticshttp://www.lifetechnologies.com/cancerepigeneticshttp://www.lifetechnologies.com/epiantibodieshttp://www.lifetechnologies.com/epiantibodieshttp://www.lifetechnologies.com/cancerrnahttp://www.lifetechnologies.com/cancerbiomarkershttp://www.lifetechnologies.com/cancerbiomarkershttp://www.lifetechnologies.com/cancerrnahttp://www.lifetechnologies.com/epiantibodieshttp://www.lifetechnologies.com/epiantibodieshttp://www.lifetechnologies.com/cancerepigeneticshttp://www.lifetechnologies.com/cancerepigeneticshttp://www.lifetechnologies.com/cancergenotypehttp://www.lifetechnologies.com/drugdiscoveryhttp://www.lifetechnologies.com/immunotherapyresearchhttp://www.lifetechnologies.com/immunotherapyresearchhttp://www.lifetechnologies.com/cancerstemcellshttp://www.lifetechnologies.com/cancerstemcellshttp://www.lifetechnologies.com/attunehttp://www.lifetechnologies.com/genearthttp://www.lifetechnologies.com/rnaihttp://www.lifetechnologies.com/cellculturehttp://www.lifetechnologies.com/abfinityhttp://www.lifetechnologies.com/immunoassayhttp://www.lifetechnologies.com/premochameleonhttp://www.lifetechnologies.com/premochameleonhttp://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Cell-Viability-and-Regulation/Cell-Cycle/Live-Cell-Imaging-of-Cell-Cycle-and-Division.htmlhttp://www.lifetechnologies.com/floidhttp://www.lifetechnologies.com/cellviabilityhttp://www.lifetechnologies.com/cellviability
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Stem cellresearch
Stem cell researchThe discovery in 2006 that human and mouse fibroblasts could be reprogrammed
to generate induced pluripotent stem cells (iPSCs) with qualities remarkably
similar to embryonic stem cells has created a valuable new source of pluripotent
cells for drug discovery, cell therapy, and basic research.
Life Technologies™ products have been an integral part of stem cell research from
before the discovery of iPSCs to current breakthroughs. With Gibco® stem cell
culture products, Invitrogen™ stem cell reprogramming products, and a wide array
of stem cell analysis tools that include Molecular Probes® labeling and detection
reagents, we have the products to support you at every step of your research.
Stem cell research can be time-consuming—finding support shouldn’t beLife Technologies gives you more PSC resources than any other source—easy
access to more than 70 proven protocols, >2,500 peer-reviewed publications, how-to videos, and webinars from leaders in thestem cell community. And when you have a question, technical support is always available by phone, email, live chat, or in person.
We offer resources for areas of stem cell research from culture to reprogramming and tools for stem cell analysis and detection.
• Culture—4 media options, >20 protocols, and 50 years of innovation
• Reprogramming—3 reprogramming tools, 9 protocols, 4 webinars, and scientists standing by
• Detection—2 iPSC imaging methods, 1 instrument, and >30,000 colleagues
For more information about our resources for stem cell research, go to lifetechnologies.com/pscresources.
CultureWhether you’re studying stem cells for basic research, drug discovery, or therapeutic applications, Life Technologies offers an
extensive range of Gibco® media, supplements, and reagents to meet all of your stem cell culture needs. You can culture and
expand stem cells in cGMP-manufactured serum-free, feeder-free, or xeno-free cell culture systems. Manufactured with superiorquality standards, our products offer the consistency and accuracy you need.
Find out about all of the options for PSC culture at lifetechnologies.com/culturepsc.
ReprogrammingSelecting the right reprogramming tool to generate iPSCs can leave you with questions. And no one has more answers
than Life Technologies. From traditional lentivirus methods to nonintegrating technologies like the CytoTune®-iPS Sendai
Reprogramming Kit and episomal iPSC reprogramming vectors, our products provide more solutions to meet your safety, efficiency,
and budgetary needs.
Find out about all of the options for reprogramming at lifetechnologies.com/cellreprogram.
DetectionValidation is critical for iPSC research. Whether you want to quickly confirm that cells are pluripotent or need flexibility in antibody
and dye choice, Molecular Probes® labeling and detection assays and imaging stations help you get the confirmation you need.
And, all of our labeling and detection technologies are backed by our extensive internal and external support resources, including
the Molecular Probes® online community and the trusted Molecular Probes® Handbook.
Find out about all of the options for detection at lifetechnologies.com/detectpsc.
Cell biology research and pathways
http://www.lifetechnologies.com/pscresourceshttp://www.lifetechnologies.com/culturepschttp://www.lifetechnologies.com/cellreprogramhttp://www.lifetechnologies.com/detectpschttp://www.lifetechnologies.com/detectpschttp://www.lifetechnologies.com/cellreprogramhttp://www.lifetechnologies.com/culturepschttp://www.lifetechnologies.com/pscresources
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Culture systems
KnockOut™ Serum Replacement—well-referenced supplement forfeeder-dependent growth of PSCs
StemPro® hESC SFM—medium for feeder-free growth of human PSCsthat allows optimization of basic fibroblast growth factor concentration
Essential 8™ Medium—optimized 8-component medium for feeder-freegrowth of human PSCs
KnockOut™ SR XenoFree Kit—feeder-free and xeno-free medium forhuman PSCs, with documentation for cell therapy applications
Reprogramming systems
Lentiviral particles—flexible technology for standard reprogramminglifetechnologies.com/lenti
Episomal iPSC reprogramming vectors—nonintegrating, nonviraltechnology for iPSC generation
CytoTune®-iPS Sendai Reprogramming Kit—nonintegrating technologyfor standard and difficult cell types
Detection systems
Alkaline Phosphatase Live Stain—allows for a quick check of pluripo-tency without compromising cell integrity
Antibodies—options for specific staining and visualization of stem cellmarkers with maximum flexibilitylifetechnologies.com/antibodies
FLoid® Cell Imaging Station—benchtop instrument for easy visualizationof Alkaline Phosphatase Live Stain and antibodies
Cell biology research and pathways
http://www.lifetechnologies.com/lentihttp://www.lifetechnologies.com/antibodieshttp://www.lifetechnologies.com/antibodieshttp://www.lifetechnologies.com/lenti
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Plantcellbiologyresearch
Plant cell biology researchUnderstanding the structural and functional relationships of plant cells and tissues is critical to plant research.
From studying the morphology of plant cells and walls, organelles, and other cellular compartments all the way
to complex gene regulation and expression studies, cell tissue analysis techniques and tools are critical not only
for basic plant research but also for programs of crop improvement. For example, demonstrating the mecha-
nism of action of a new trait as part of the regulatory package is critical in trait development of new commercial
plant varieties. Key techniques such as immunohistochemistry have been routinely used to label diverse plant
tissues and organelles including cell walls, vacuoles, chromatin, nuclei, nuclear membranes, and chloroplasts.
To empower your research in plant cell and tissue analysis, Life Technologies has a broad portfolio of fluorescent
reagents, kits, and instruments specifically designed with your needs in mind.
Molecular Probes® fluorescent dyes and probesMolecular Probes® reagents including Alexa Fluor® dyes, Qdot® nanocrystals, Click-iT® detection assays, and
ProLong® Gold antifade reagent are used in a wide range of plants, for a variety of plant research applications.
For example:
Rubisco localization in maize leaf section. Rubisco waslocalized using a rabbit anti-rubisco antibody and visu-alized using the highly cross-adsorbed Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11034).The 2.0 µm maize leaf section illustrates the immuno-localization of rubisco in the chloroplasts of the bundlesheath cells surrounding the vascular bundles. Thered fluorescence, localized to the mesophyll plastids,is due to background autofluorescence of chlorophyll.
Lignin appears dull green and is localized to the xylemof the vascular bundle; cutin appears bright green andis localized to the cuticle outside the epidermis. Imagecontributed by Todd Jones, DuPont.
Pectin associated with plasmodesmatal pit fields ofkiwifruit cells. Pectin, a component of the cell wallmatrix and the main constituent of the middle lamellathat forms between daughter cell walls, was taggedwith an anti-pectin monoclonal antibody, JIM 5. Theprimary antibody was detected and visualized withAlexa Fluor® 488 goat anti–rat IgG (Cat. No. A11006).The primary antibody was a gift from Dr. Paul Knox,University of Leeds, UK. Image contributed by Paul
Sutherland, The Horticulture and Food Research Insti-tute of New Zealand, Ltd., Mt. Albert Research Centre.
Ca2+ gradient in elongating lily pollen tube. Top panel:Pseudocolored image of a pollen tube of Lilium longi-florum injected with fura dextran (Cat. No. F3029).The cell continues elongating and clearly shows aCa2+ gradient. Bottom panel: The same pollen tubeafter injection with dibromo-BAPTA (Cat. No. D1211)remains healthy but is no longer elongating. Imagescontributed by Debra Miller, Dale Callaham, DavidGross, and Peter Hepler, University of Massachusetts.
• Immunohistochemistry• Fluorescence in situ hybridization
• Organelle and cytoskeletal stains
• Endocytosis studies
• Transport studies
• Glucose metabolism• Viability staining
• Glutathione detection
• Calcium and other ion imaging
Cell biology research and pathways
http://products.invitrogen.com/ivgn/product/A11034http://products.invitrogen.com/ivgn/product/A11006http://products.invitrogen.com/ivgn/product/F3029http://products.invitrogen.com/ivgn/product/D1211http://products.invitrogen.com/ivgn/product/D1211http://products.invitrogen.com/ivgn/product/F3029http://products.invitrogen.com/ivgn/product/A11006http://products.invitrogen.com/ivgn/product/A11034
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Flow cytometryFlow cytometry represents an ideal means for the analysis of both cells and subcellular
particles, with a potentially large number of parameters analyzed rapidly, simultaneously,
and quantitatively, thereby furnishing statistically exploitable data and allowing accurate
and facilitated detection of subpopulations. Since the first report of the use of this tech-
nique in plants 40 years ago, flow cytometry has become an essential tool for the under-
standing of fundamental mechanisms and processes underlying plant growth, develop-ment, and function. Key applications include the study of ploidy levels, genome mapping,
localization of sex-specific chromosomes, and plant cell and algal studies.
Life Technologies offers a wide range of flow cytometric analysis tools including the
Attune® Acoustic Focusing Cytometer and fluorescent probes.
Learn more and see all of our products at lifetechnologies.com/probes.
Featured product: Alexa Fluor® dyesThe leading and most trusted fluorescent dyes available today have been used extensively
in the plant sciences. In addition to superior performance, you’ll get an experienced,
problem-solving technical support team, over 30,000 published references, application
and experimental tips, and protocols to help with experimental planning. Our range oflabeling kits, molecules for tracing cell structures, and secondary detection reagents
within the Alexa Fluor® product line provide superior brightness and photostability,
outperforming conventional fluorescent reagents.
Learn more at lifetechnologies.com/alexa.
For a comprehensive view of all your products and technologies to empower your plant
sciences research, visit lifetechnologies.com/plants.
Nuclear holoploid genome sizing analysis of Arabidopsis thaliana leaf tissue homogenates using the Attune® AcousticFocusing Cytometer. (A) Biparametric density plot of side scatter vs. propidium iodide (PI) fluorescence with Scattergate surrounding the fluorescent nuclei. (B) Biparametric density plot of PI fluorescence (603/48 vs. ≥640 nm) ofScatter gate population from A. (C) Logarithmic histogram of PI fluorescence (603/48 nm) of Nuclei gate populationfrom B. (D) Linear histogram of PI fluorescence (603/48 nm) of Nuclei gate population from B. 2C, 4C, 8C, 16C, and32C denote the C-value for the respective peaks.
PI 603-48 BL2-A
S S C - A
103 104 105 106103
104
105
106
PI 603-48 BL2-A
P I 6 4 0
L P B
L 3 - A
104 105 106103
104
105
106
PI 603-48 BL2-A
C o u
n t
104 105 1050
100
200
300
400
500
600
700
PI 603-48 BL2-A (10^3)
C o u
n t
0 50 100 150 200 250 3000
100
200
300
400500
600
700
800
900
1,000
2C
4C2C
4C
8C
16C
8C
16C
32C
Scatter
Nuclei
Cell biology research and pathways
A
C
B
D
http://www.lifetechnologies.com/probeshttp://www.lifetechnologies.com/alexahttp://www.lifetechnologies.com/plantshttp://www.lifetechnologies.com/plantshttp://www.lifetechnologies.com/alexahttp://www.lifetechnologies.com/probes
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AKTpathway
AKT pathwayThe serine-threonine kinase AKT (also known as protein kinase B) is a protein that acts as a central conver-
gence node in a broadly influential signaling network. AKT activation serves as a master switch of these
cellular signaling pathways, generating a multitude of intracellular responses through a plethora of down-
stream targets and interacting partners. Because of its pivotal role in cell signaling, and the consequences
of that signaling in diseases ranging from cancer and diabetes to neurodegeneration, AKT is one of the most
actively studied kinases in both basic research and drug development. Here we highlight a few of our prod-
ucts for analyzing the AKT signaling pathway and its downstream targets.
Life Technologies offers Novex® antibodies, ELISAs, Novex® assays for the Luminex® platform, and growth
factors for key targets in the AKT signaling cascade.
Key targets of interest in the AKT pathway
Protein Characteristics Means of regulation Research tools (Chapter 4, pages 146-155)
AKT/protein kinase B Master regulator of cell growth andsignaling cascade
Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
mTOR Cell growth regulator Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
GSK-3B Cell cycle regulator Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
MDM2 Negative regulator of p53 function Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
NF-κB Transcriptional activator regulatingapoptosis
Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
Additional references
Madonna R, Bolli R, Rokosh G et al. (2012) Targeting phosphatidylinositol 3-kinase-Akt through hepatocyte growth factor for
cardioprotection. J Cardiovasc Med doi: 10.2459/JCM.0b013e3283542017.Papadimitrakopoulou V (2012) Development of PI3K/AKT/mTOR pathway inhibitors and their application in personalized therapyfor non-small-cell lung cancer. J Thorac Oncol doi: 10.1097/JTO.0b013e31825493eb.
Wang G, Pan J, Chen SD (2012) Kinases and kinase signaling pathways: potential therapeutic targets in Parkinson’s disease. ProgNeurobiol 98(2):207–221.
Sheppard K, Kinross KM, Solomon B et al. (2012) Targeting PI3 kinase/AKT/mTOR signaling in cancer. Crit Rev Oncog 17(1):69–95.
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p85 p110
mTORC1
GβL rictormTOR
mTORC2
GβL raptormTOR
Rapamycin
CCND1
p21
β-CTN
IKK
TSC1
TSC2
NFAT
PRAS40
p27
Rheb
IκB
NFκB
4E-BP1
eIF4E
eIF2BeIF4A
MDM2
BAD
ASK-1
CASP9
p21
FAS
PTEN
CIP1FAS-L
p27
KIP1
RTK
PTEN
PIP3
PDK1
Growth factor
PIP2
FOXO
IkB
AR
RPS6
eIF4B
PDCD4
SK6
FKBP
Cell cycle
AKT
Apoptosis
Cell growth
Cell proliferation
GSK-3β
Transcription
Translationinitiation
Glycogensynthesis
Cytoskeletalreorganization
PI3K
PP2A
α4
FOXO
p53
NFATGATA4 NFκB
Phosphatase
Hormone receptor
Kinase
Translation factor
Receptor tyrosine kinase
Transcription factor
+P
+P
+P
+P
+P +P
+P
+P
+P
+P
+P
+P
+P
+P
+P
+P
+P
+P
+P+P
+P
+P
+P
+P
-P
-P
The AKT pathway.
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MAPKpathway
MAPK pathwayThe mitogen activated protein kinase (MAPK) pathway is central to cellular signal transduction in response
to growth factors, mitogens, cytokines, and stress. The MAPK pathway is regulated through a series of
reversible phosphorylation events resulting in a variety of cellular activities including growth, differentia-
tion, development, and apoptosis. Here we highlight a few of our products for analyzing the MAPK signaling
pathway and its downstream targets.
Life Technologies offers Novex® antibodies, ELISAs, Novex® assays for the Luminex® platform, and growth
factors for key targets in the MAPK signaling cascade.
Key targets of interest in the MAPK pathway
Protein Characteristics Means of regulation Research tools (Chapter 4, pages 146-155)
MAPK Regulator of cell growth and signalingcascade
Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assays forthe Luminex® platform
ERK Regulator of growth, development, andpoliferation
Phosphorylation/dephosphorylation
Novex® antibodies, ELISAs, and Novex® assays forthe Luminex® platform
JNK Regulator of inflammation, apoptosis,and differentiation Phosphorylation/dephosphorylation Novex®
antibodies, ELISAs, and Novex®
assays forthe Luminex® platform
Ras GTPase involved in cell proliferation Cleavage of GTP Novex® antibodies, ELISAs, and Novex® assays forthe Luminex® platform
Additional references
Oeztuerk-Winder F, Ventura JJ (2012) The many faces of p38 mitogen-activated protein kinase in progenitor/stem cell differentia-tion. Biochem J 445(1):1–10.
Gantke T, Sriskantharajah S, Sadowski M et al. (2012) IκB kinase regulation of the TPL-2/ERK MAPK pathway. Immunol Rev 246(1):168–182.
Pópulo H et al. (2012) The mTOR signalling pathway in human cancer. Int J Mol Sci 13(2):1886–1918.
Aksamitiene E, Kiyatkin A, Kholodenko BN (2012) Cross-talk between mitogenic Ras/MAPK and survival PI3K/Akt pathways: a finebalance. Biochem Soc Trans 40(1):139–146.
Davies C, Tournier C (2012) Exploring the function of the JNK (c-Jun N-terminal kinase) signalling pathway in physiological andpathological processes to design novel therapeutic strategies. Biochem Soc Trans 40:85–89.
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The MAPK pathway.
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JAK/STATpathway
JAK/STAT pathwayThe Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays a central role in
transducing signals from a wide array of cytokines and growth factors leading to various cellular functions,
including proliferation, growth, development, hematopoiesis, and immune responses.
Abnormal constitutive activation of the JAK/STAT pathway has been implicated in various cancers and immunedisorders. Thus, inhibitors of the JAK/STAT pathway are being sought.
Here we highlight a few of our products for analyzing the JAK/STAT signaling pathway and its downstream targets.
Life Technologies offers Novex® antibodies, ELISAs, Novex® assays for the Luminex® platform, and growth
factors for key targets in the JAK/STAT signaling cascade.
Key targets of interest in the JAK /STAT pathway
Protein Characteristics Means of regulation Research tools (Chapter 4, pages 146-155)
JAKs Initiate signaling upon receptormultimerization
Phosphorylation/dephosphorylation Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
STATs Transcription factors Phosphorylation/dephosphorylation,DNA binding Novex®
antibodies, ELISAs, and Novex®
assaysfor the Luminex® platform
SOCS Regulator of JAK signaling Binding of JAK Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
PIAS Regulator of STAT signaling Binding of STAT Novex® antibodies, ELISAs, and Novex® assaysfor the Luminex® platform
Additional references
Harrison DA (2012) The Jak/STAT pathway. Cold Spring Harb Perspect Biol 4(3). pii: a011205. doi: 10.1101/cshperspect.a011205.
Sansone P, Bromberg J (2012) Targeting the interleukin-6/Jak/stat pathway in human malignancies. J Clin Oncol 30(9):1005–1014.
Wagner KU and Schmidt JW (2011) The two faces of Janus kinases and their respective STATs in mammary gland development andcancer. J Carcinog 10:32. doi: 10.4103/1477-3163.90677.
Mohr A, Chatain N, Domoszlai T et al. (2012) Dynamics and non-canonical aspects of JAK/STAT signalling.Eur J Cell Biol 91(6–7):524–532.Tamiya T, Kashiwagi I, Takahashi R et al. (2011) Suppressors of cytokine signaling (SOCS) proteins and JAK/STAT pathways: regulationof T-cell inflammation by SOCS1 and SOCS3. Arterioscler Thromb Vasc Biol 31(5):980–985.
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The JAK/STAT pathway.
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Neurodegeneration
NeurodegenerationNeuronal death is pivotal to Parkinson’s disease (PD) pathogenesis and can be triggered by various cellular
mechanisms, including oxidative stress, misfolding of proteins, mitochondrial dysfunction, and proteasome
dysfunction. In their pursuit to fully understand the various pathways that lead to neurodegenerative diseases
such as PD, researchers are exploring the molecular basis of these mechanisms. Here we highlight just a
few of our products for analyzing the key factors and effectors in PD.
Life Technologies offers Novex® antibodies, ELISAs, Novex® assays for the Luminex® multiplex assays
platform, and growth factors for key targets in studying Parkinson’s disease.
Some key targets of interest are listed in the table below.
Protein Characteristics Means of regulation Research tools (Chapter 4, pages 146-155)
Parkin Mitochondrial health Mutation and misfolding Novex® antibodies
α-Synuclein Mitochondrial health Aggregation and mutationNovex® antibodies, ELISA, and Novex® assays forthe Luminex® platform
LRRK2 Leucine-rich kinase Phosphorylation/dephosphorylationNovex® antibodies, ELISA, and Novex® assays for
the Luminex®
platform
TauNucleation and stabilizationof microtubules
Phosphorylation/dephosphorylation/misfolding
Novex® antibodies, ELISA, and Novex® assays forthe Luminex® platform
Additional references
Protter D, Lang C, Cooper AA (2012) αSynuclein and mitochondrial dysfunction: a pathogenic partnership in Parkinson’s disease?Parkinsons Dis 2012:829207.
Sutachan JJ, Casas Z, Albarracin SL et al. (2012) Cellular and molecular mechanisms of antioxidants in Parkinson’s disease. NutrNeurosci 15(3):120–126.
Harvey L, Boksa P (2012) Prenatal and postnatal animal models of immune activation: Relevance to a range of neurodevelop-mental disorders. Developmental Neurobiology special issue: Neuroimmunology in Development and Disease. Dev Neurobiol doi:10.1002/dneu.22043.
Wang G, Pan J, Chen SD (2012) Kinases and kinase signaling pathways: potential therapeutic targets in Parkinson’s disease. ProgNeurobiol 98(2):207–221.
Rademakers R, Neumann M, Mackenzie IR (2012) Advances in understanding the molecular basis of frontotemporal dementia.Nat Rev Neurol doi: 10.1038/nrneurol.2012.117.
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Gibco
custom media
services
Gibco® custom media servicesDelivering speed, scalability, and qualityScalability is critical for clinical and biomanufacturing applications. Our tech transfer process helps ensure smooth scale-up as
batch size increases.
Small-scale media production when speedmatters most
Large-scale media production in our world-class, validated manufacturing facilities
Choose Gibco® MediaExpress for small-scale media
production:
• Fast turnaround (typically
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Custom Novex multiplexassaysforLuminex tec
hnology
Custom Novex® multiplex assays forLuminex® technologyNew made-to-order assay design system: design and place an order orget a quote onlineOur custom Novex® multiplex immunoassay kits for the Luminex® platform maximize flexibility in experimental design, permit-
ting the quantitation of one or multiple targeted proteins in unique panels designed by you. For your convenience, each custom-
ized panel comes with reagents that are blended, optimized, tested, and designed for use with the Luminex® 100/200™ system,
FLEXMAP 3D® system, and the new MAGPIX® system.
Target Species Bead type
Cytokine Human Magnetic
Chemokine Mouse Polystyrene
Growth factor Rat
Signaling protein Nonhuman primate
Design and price your own Novex® multiplex assay kit at bioinfo.invitrogen.com/luminex .
Choose specific targets of interest(polystyrene or magnetic)
Combined with appropriateBuffer Kit (all needed reagents)
for ease of use
Each assay is premixed and QC testedfor optimum top signal, low
background, and linearity of dilution
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Cell biology resources
Custom biology servicesDrug discovery demands custom research and custom solutionsFrom cell engineering to custom assay development, our scientists work closely with your team to understand your goals and tailor
a solution that fits your unique project guidelines.
When an off-the-shelf tool isn’t the right option, our custom biology group offers unique and highly efficient solutions to help ensure
you stay on track. Expect consistently short cycle times and high-quality results on time, every time, with proactive communication
throughout the project.
Custom assays and services• Custom biochemical and cell-based assays—Let Life Technologies create a
custom biochemical or cell-based solution to suit your needs, using the broad
array of technologies in the Life Technologies toolbox.
• Cell line generation—Outsource your cell line development and let us craft a
Jump-In™ parental cell line or final engineered cell line that meets your defined
specifications.
•
Antibody labeling—Choose from europium or terbium donor chelate labels andobtain labeled antibodies for your TR-FRET assay development projects.
• Cell provisioning—Get validated, high-throughput screening–ready cells in large
scale with our cryopreservation service.
• siRNA profiling—Identify and validate your target of interest using first-in-class
siRNA collections with readouts to interrogate functional pathways, proliferation,
gene expression analysis, and protein modifications.
• BacMam virus production assays—Let us create a BacMam virus from your gene
of interest or develop a portable and robust BacMam-enabled assay.
• TaqMan® protein assays—Get highly sensitive protein detection coupling the speci-
ficity of antibody affinity reagents with the sensitivity and fidelity of TaqMan® PCR.
Tell us your custom assay requirements at lifetechnologies.com/custom.
Biochemical and cellular profiling servicesDrug discovery scientists have trusted the SelectScreen® Services team for thousands
of screening and profiling projects on more than 500 targets.
SelectScreen® Services are executed with strict quality controls using a state-of-the-
art compound management system to drive down cycle times and meet expanding
customer demand. Highly trained personnel and automated processes driven by inte-
grated informatics systems help us ensure that reliable, high-quality data are deliv-
ered every time.
• SelectScreen® Kinase Profiling Service—profile with our expanding panel of
distinct protein kinases and get an average delivery time of just 6 days.
• SelectScreen® Cell-Based Pathway Profiling Service—understand potentialoff-target effects earlier with our rapid, flexible cell-based profiling service against
a panel of pathway-specific CellSensor® cell lines.
• SelectScreen® Cell-Based GPCR Profiling Service—choose from a panel of
functionally validated GeneBLAzer®, second-messenger, and Tango™ beta-
arrestin recruitment cell-based assays.
• SelectScreen® Cell-Based Nuclear Receptor Profiling Service—profile your
compounds against the most comprehensive panel of target-specific Gene-
BLAzer® nuclear hormone receptor assays for early safety and liability testing.
Choose the right profiling service for your research at lifetechnologies.com/selectscreen.
http://www.lifetechnologies.com/customhttp://www.lifetechnologies.com/selectscreenhttp://www.lifetechnologies.com/selectscreenhttp://www.lifetechnologies.com/custom
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Cell biology resources
Protocol DatabaseThe Protocol Database is a Web-based tool that allows researchers to find more than 500 validated proto-
cols for performing critical cell biology experiments. Protocols include culturing primary or stem cells,
performing imaging and other cell analysis techniques, and protein purification, separation and identifica-
tion techniques. The database allows the user to search for a specific protocol using keywords or to browse.
The database can be found at lifetechnologies.com/protocolsdatabase.
The Molecular Probes®
Handbook onlineThe online version of The Molecular Probes® Handbook (lifetechnologies.com/handbook ) is a comprehensive
resource for fluorescence technology that is regularly updated. Like the printed version, the online Handbook
contains detailed information about the thousands of Molecular Probes® products available for fluorescence
and imaging applications. The online Handbook has product pricing for your region as well as the added
advantage of having critical product information at your fingertips. Find easy access to data images and
referenced citations as well as product structures and spectra.
http://www.lifetechnologies.com/protocolsdatabasehttp://www.lifetechnologies.com/handbookhttp://www.lifetechnologies.com/handbookhttp://www.lifetechnologies.com/protocolsdatabase
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FluorescenceSpectr
aViewer
FluorescenceSpectraViewerThe Fluorescence SpectraViewer is an online tool that can be
used to plot and compare the excitation and emission spectraof fluorescent dyes, facilitating the design of multicolor fluo-
rescence experiments. As many as five fluorophores can be
selected from an extensive list of dyes for simultaneous spec-
tral analysis to determine the suitability of fluorophores for
specific instrument configurations, and to evaluate spectral
compatibility of different fluorophores. The SpectraViewer is
available at lifetechnologies.com/spectraviewer .
Mobile applicationsand widgetsLife Technologies offers an assortment of handy applications
and widgets for your everyday mobile needs. Download popular
iPhone® apps and widgets from Molecular Probes and other
Life Technologies brands, including the DailyCalcs Science
Calculator and the Alexa Fluor® Selection Guide widget. Visit
lifetechnologies.com/apps to get started. New apps and
widgets are introduced on a regular basis, so check back often.
Virtual CellStaining ToolThe Virtual Cell Staining Tool (lifetechnologies.com/
cellstainingtool) allows you to select different combinations of
cellular structures and fluorophores to create your perfectly
labeled fluorescent cell, which you can easily share with your
colleagues using our email or print functionality.
http://www.lifetechnologies.com/spectraviewerhttp://www.lifetechnologies.com/appshttp://www.lifetechnologies.com/cellstainingtoolhttp://www.lifetechnologies.com/cellstainingtoolhttp://www.lifetechnologies.com/cellstainingtoolhttp://www.lifetechnologies.com/cellstainingtoolhttp://www.lifetechnologies.com/appshttp://www.lifetechnologies.com/spectraviewer
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Cell biology resources
Customer andtechnical supportComprehensive worldwide supportWhether you need help with a current order, placing a new order, or finding more infor-
mation about product availability, please contact your local customer service team.
Technical support and trainingIf you have questions about product selection or use, assay or experimental design,
data analysis, or troubleshooting, contact our team of technical support scientists or
utilize our comprehensive portfolio of online product and application support tools.
Follow us on Twitter:
@LifeTechSupport
@AppliedBio
@dynabeads
@everydayprotein
@gibco
@invitrogen
@molprobes
@The_RNA_experts
@lifeimmunology
@LifetechEMEA
Like us on Facebook:
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LSIA 2011 Awards – Most ResponsiveCustomer Service & MostKnowledgeable Technical Support
How to reach usTo find your local customer service or technical support team, go to lifetechnologies.com/contactus.
For product FAQs, protocols, training courses, and webinars, go to lifetechnologies.com/technicalresources.
Antibody and immunoassayselection guides
Product selection guide Purpose
Immunoassay SelectorTool
Find the right Novex® immunoassay product easily using our convenient selection tool. Simply search by keyword,then filter your results to find the right ELISA kit, Luminex® platform assay, or antibody pair.
http://igene.invitrogen.com/products/selector/immunoassays
Labeling ChemistrySelector Tool
Quickly find the best Molecular Probes® reactive dye or label for your experiment. Whether you want to labelproteins and antibodies for immunofluorescence with our Alexa Fluor® dyes, nucleic acids for in situ hybridization,or lipids for membrane studies, this tool will help you find the right conjugation product.
http://igene.invitrogen.com/products/selector/dyes
Primary AntibodySelector Tool
Find all of the Life Technologies primary antibodies, including Molecular Probes® flow cytometry antibodies,Novex® primary antibodies for western blot analysis, and more. Simply search by target, gene symbol, or gene ID,then filter your results by application, reactivity, host, conjugate type, or antibody type. You can also search withinyour results to quickly find the right antibody.
http://igene.invitrogen.com/isearch
Secondary AntibodySelector Tool
Finding the right secondary antibody is easier than ever. Whether you are looking for secondary antibodies,anti-dyes and anti-haptens, anti-epitope tags and anti-reporter genes, or isotype controls, Life Technologies hasthe largest selection available. Simply select the type of antibody you are looking for and filter by target, Ig class,reactivity, conjugate, or host.
http://igene.invitrogen.com/antibody
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Cell culture and cells
SectionPrimary andstem cells
Recover Grow
Highlightedproducts
• Primary human
keratinocytes,
broblasts, corneal
epithelial cells,
umbilical vein endo-
thelial cells, and
more, page 56
• Neural primary and
stem cells, page
56, 71
• Mesenchymal stem
cells, page 68
• Hematopoietic stem
cells, page 73
• Recovery™
Freezing Medium
results in 25%
more viable
cells post-thaw
and requires
no additionalsupplementation,
page 40
• Gibco® FreeStyle™ protein expression systems provide rapid, scalable, high-
yield transient expression in both 293 and CHO cells, page 42
• Gibco® CD Hybridoma Medium is a chemically dened, animal origin–free
medium optimized for the growth of hybridomas, page 42
• Gibco® Sf-900 III™ medium improves the consistency of your insect cell
culture, page 47• Choose Geltrex®, AlgiMatrix™, or CellStart™ CTS™ 3D cell culture matrices
to get more physiologically relevant culture conditions, page 49
• Essential 8™ Medium offers optimal feeder-free culture for all human PSC
and iPSC lines with minimal lot-to-lot variability, page 60
• StemPro® NSC SFM is a serum-free medium for neural stem cells, page
52, 63
• EpiLife® medium for enhanced primary keratinocyte cell culture, page 57
• Cell Therapy Systems (CTS™) validated reagents for clinical research appli-
cations, look for the CTS™ label throughout this chapter
Everydayessentials
• Classical Gibco® media, cells, sera, and reagents for general cell culture
in a wide variety of formats and sizes. Choose from standard media such
as DMEM, RPMI, and MEM to more advanced formulations including
GlutaMAX™, OptiMEM™, or Advanced™ reduced-serum media, page 41
• Gibco® provides FBS for both general and specialized cell culture, page 43
• Discover a broad range of eukaryotic and bacterial selection antibiotics such
as Geneticin®, puromycin, and Zeocin™, page 46
• Gibco® growth factors are manufactured for high activity, purity, and
compatibility with Gibco® media, page 47
• B-27® Supplement, the most published serum substitute for neural cell
culture, page 72
• Neurobasal® media, basal media formulated to sustain optimal culture of
neurons when used with B-27®, N-2, or G-5 supplements, page 71
• KnockOut™ Serum Replacement, the standard supplement for growing
pluripotent stem cells from multiple spe