8/11/2019 Cell Bio Report 1: Microscopy

1/3

Laboratory Report 1: The microscope

By: Murad Jabarov

Student ID: 500456223

Section: Number 10

Professor: Dr. Antonescus

8/11/2019 Cell Bio Report 1: Microscopy

2/3

Introduction:

The main function of a microscope is to enlarge an image. Magnification is the number of times

by which the image is enlarged. But just enlarging the image is not enough, because then the image is

going to be blurry and we still won't be able to identify the different components of the image. For that

purpose as the image gets enlarged the resolution must be enlarged too. Resolution describes howdetailed the image is.

Microscope is mainly made out of lenses, and those lenses have a limited angle at which they

can except light. The limitation of a lens to absorb or transfer a light is described by numerical aperture.

Stage condenser is a machine located under the stage of microscope which serves to concentrate and

manipulate the light intensity before it hits the specimen. After the light passes throw the condenser

and the specimen it is absorbed by the objective lenses. Most of light microscopes have 4 main lenses

x4,x10,x40 and x100.Lenses magnify the image, allowing to see the detailed cell structures. The part

throw which the image is absorbed is called the ocular lens. The ocular lens has a magnification of 10x

and is the last step the light overcomes before being seen by the eye.

The microorganisms that are being examined in this lab are very small. Spirogyra is 60.49 m X

99.94 m while Paramecium is 71.01 m X 178.84 m. This organisms are small but with 40x lens

magnification and 10x ocular magnification they are seen pretty well. But in order to see smaller

organisms like Saccharomyces cerevisiae (wild type) which are only 5 m X 4 m or Saccharomyces

cerevisiae 7 m X 7 m one would need to use an oil emersion lens with 100x magnification. In order to

see smaller components of the cell or smaller cells higher magnification and resolution is required.

The purpose of this experiment was to learn the adequate use of microscope and techniques

associated with calibration of microscope ruler, as well as learning how to measure the size of

microorganisms. Some pipette techniques were also utilized while the lab period.

8/11/2019 Cell Bio Report 1: Microscopy

3/3

Results:

Part B: Answering questions.

1)When compared the values for dimensions of "e" they turned out to be different, although they

should have been the same. This is probably due to optical error.

2)The two differences between the organisms are that first, Paramecium does not form colonies, that is

they are not attached to one another like Spirogyra are. The second difference is in the shape of a single

cell; Paramecium appears to be in a shape of a foot print, while the Spirogyra is more rectangular.

3)Based on recording from exercise 1.4 it is concluded that pupating accuracy was good, because it was

almost 50 l and there were absolutely no air-bubbles absorbed.

4) The major difference between the wild type and fab1 of Saccharomyces cerevisiae was that the

fab1 was round rather than ovular. It was also possible to see the nucleolus in fab1 unlike in wild-

type that was entirely dark inside. Finally the fab1 was lager in size in comparison to their wild-type

relatives.

Part C: Research

I would suggest contacting "AmScope" for any kind of microscopy related purchases. The

particular microscope model is "T600A-PCS-3M", it comes with 4 optical lenses and 2 sets of ocular

lenses as well as a 3MP camera. Magnification ranges 40x-1600x. The price for this package is 1106$ (US

Dollars) + 183.38 (US Dollars) shipping to Toronto.

This information was acquired through http://www.amscope.com

I knew this website from when I bought my microscope, but I used Google.com to find it for the first

time.