Ce ll- Cu lture real time Mo nitoring system Analog Design Contest 2013

Cell-Culture real time Monitoring system

Analog Design Contest 2013

Team: José A. Maldonado, Juan N. Olmo, Gloria Huertas, Alberto Yúfera

Cecumo

“The important thing is not to stop questioning”. Albert Einstein

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AGENDA

Motivation Theoretical background

Simulations System description

Implementation Features Results

Final conclusions Questions and comments

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

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MOTIVATION


Cecumo

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THEORETICAL BACKGROUND


Z(w)/A Rs

Z(w)/(A-Ac) RsCELL

Z(w)/Ac Rgap

Low-Pass Behaviour function of cell-culture occupation area (Ac)

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HBP(s)

Zcell-electrode(s)

RinVoutVout2

THEORETICAL BACKGROUND: SIMULATIONS


Oscillation-Based Test approach

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SYSTEM DESCRIPTION: IMPLEMENTATION


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SYSTEM DESCRIPTION: RESULTS


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SYSTEM DESCRIPTION: RESULTS


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SYSTEM DESCRIPTION: FEATURES


Real time monitoring “Cell friendly” (Max. 30mV/30μA)

Immediate result Up to 8 cultures Wireless connectivity Data logging Long battery lifetime (100mA/10mA@±5V)

Compact and light-weight design (160x80x25mm)

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CONCLUSIONS

Low cost & reusable “biological” oscillator for Cell Culture growing measurements.

Amplitude and frequency to characterize occupation. AGC for better noise inmunity Frequency and AGC control voltage (Vg) to extract Fill

Factor (ff) parameter. Potential fields of application: medicine and biology


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QUESTIONS AND COMMENTS


Thank you for your attention!

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Band Pass Filter

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AGC

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BioZ Interface

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High Pass Filter & Comparator

Ce ll- Cu lture real time Mo nitoring system Analog Design Contest 2013

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