September 25, 2009

Organotypic Tissue Culture for Organotypic Tissue Culture for Substance EvaluationSubstance Evaluation

Potsdam, September 2009

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WHY WE ARE HERE (PART I)

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WHY WE ARE HERE (PART II)

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Single Organs Multiple Organs• Skin

• Liver

• Lymph Node

• Pancreas

• Heart/Muscle

• Trachea/Lung

• Gut/Intestine

• Tumor

• Single neurons

• Liver/GI

• Kidney/GI

• Neural cortex/bone marrow/stem cells/circulation

BEYOND CELLS TISSUES & ORGANS

DISCOVERIES

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• The meaning of life? Reaction and communication

• “Single cells are destined to die”

• Life is different at the micro- and nahnoscale

• Dynamics create challenges as well as opportunitieso Flow creates evaporation and removal as well as circulation and nutrition

• Skin is a pioneering exampleo Monolayer Full Thickness (FT)

o Short-term culture One year in culture!

o R&D Commercial with multiple vendors

FRONTIERS (SCIENTIFIC)

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• Use the environment more• Cross the species barrier • Go nano

o Use nanomaterials to deliver co-factors like Ca+2 o Use nanoparticles to enhance transport

• Seek to scaleo Scale down: micro- and nanogeometries may be differento Scale up: 1+1 = ½?

• Co-culture is crucial even in single-organ systems (pancreas – 4% of cells may be the key)• Adding cell types, circulation, neural connections, adipocytes – not easy but necessary

o Adipocytes to skino Stroma to pancreas; stroma to lymph nodeo Endothelial cells to lymph node

FRONTIERS (COMMERCIAL)

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• Not just normal but also disease models• Think not only “in vitro” but “in silico”• Think “manufacturability”• Think “modular”• Think “multiple price points”• Find immediate needs to satisfy • Seed it with stem cells & iPS cells

A PATH FORWARD

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Intel chip ca. 1971 Intel chip 2010

ChemistryBiologyTechnology

September, 2009

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