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Automated Liquid HandlingProduct Guide

LT303041-11 © 2011 Gilson Inc. All rights reserved. All trademarks are the property of Gilson Inc. and its subsidiaries.

Gilson, Inc. World Headquarters | P.O. Box 620027 | Middleton, WI 53562-0027 | Tel: 800-445-7661 | Fax: 608-831-7661

Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE | Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020

www.gilson.com | [email protected] | [email protected] | [email protected] | [email protected] | [email protected]

Featuring the NEW

PLC 2020 Personal Purification System (See pages 3– 4)

Autom

ated Liquid Handling | Product G

uide

PLC 2020 Purification System 3

LC/MS Purification System 5

HPLC Systems 7Gilson HPLC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8GX-281 Preparative HPLC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9GX-281 Analytical to Semi-preparative HPLC System . . . . . . . . . .10GX-271 Preparative HPLC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11GX-271 Analytical to Semi-preparative HPLC System . . . . . . . . . .12845Zprep™ Semi-preparative to Preparative HPLC System . . . . . .13cLC™ Analytical to Preparative HPLC System . . . . . . . . . . . . . . . . . . .13GX-271 Fraction Trapping System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

HPLC Liquid Handlers & Injectors 15GX-281 Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16GX-271 Preparative Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . .17GX-271 Analytical Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . . . .18215 Liquid Handler/Injector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

HPLC Pumps 20333/334 Prep-scale HPLC Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21331/332 Semi-preparative HPLC Pumps . . . . . . . . . . . . . . . . . . . . . . . .22321/322 Analytical to Semi-preparative HPLC Pumps . . . . . . . . . .2230X Series HPLC Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23402 Syringe Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23Minipuls Evolution® Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . .24Minipuls 3 Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24

HPLC Detectors 25171/172 Diode Array Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2615X Series UV/VIS Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26112 UV Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27prepELS™ II Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

HPLC Fraction Collectors 28prepFC™ Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29FC 203B/FC 204 Fraction Collectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30

Control Software 31Trilution® LC Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32Trilution® LH Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

GPC Clean-up Systems 34Automated GX-271 GPC Clean-up System . . . . . . . . . . . . . . . . . . . . . .35GX-271 GPC/SPE Clean-up System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36Manual GPC Clean-up System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

Solid Phase Extraction Systems 38GX-271 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39GX-274 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40Quad-Z 215 Liquid Handler for SPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Liquid Handlers 42GX-271 Analytical Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43GX-271 Preparative Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44GX-274 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45GX-281 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46Quad-Z 215 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47215 Liquid Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48223 Sample Changer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48

Accessories 49VALVEMATE® Valve Actuator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50Orbital Shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51RackCount™ System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51818 AutoMix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52Racks – Standard & Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52

Manual Liquid Handling 53PIPETMAN® M . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53PIPETMAN® Neo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55PIPETMAN® Neo Starter Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55PIPETMAN® Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57Trackman® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

Resources 63OEM Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63Gilson Knowledge Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64Gilson Publications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65Technical Notes Handbooks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66

Gilson Applications 67Environmental Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67Food & Beverage Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68Clinical & Forensic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69Pharmaceutical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70

References 71

Contents

About Gilson, Inc.Designed for purification and engineered for science, Gilson, Inc . provides high quality, dependable solutions for today’s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements . From the legendary Gilson PIPETMAN® products to a full range of system solutions, Gilson is the element of purification for your laboratory . The Gilson line of innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease-of-use the industry has come to expect from Gilson .

Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets . Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up . Gilson focuses on early drug discovery and development with our preparative HPLC systems . Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications .

Gilson offers complete solutions including the revolutionary control software, Trilution® . Trilution® LC Software is a complete HPLC software package designed specifically to meet purification needs . Trilution® LH Software is designed to make automation of liquid handling and SPE applications simple and dependable .

Gilson, Inc . world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U .S . Gilson S .A .S ., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products . Gilson solutions are sold and supported worldwide .

Table of Contents

2Gilson, Inc . | 800-445-7661 | www .gilson .com

Gilson, Inc. World Headquarters | P .O . Box 620027 | Middleton, WI 53562-0027 | Tel: 800-445-7661 | Fax: 608-831-7661


www.gilson.com | sales@gilson .com | service@gilson .com | training@gilson .com | hr@gilson .com | oem@gilson .com

Easy-to-Use Software

Intuitive touch screen interface allows you to be purifying compounds in minutes .

Real-time, graphical gradient modifications and real-time sample tracking during run .

Conditional logic fraction collection based on up to two detector channels using any combination of Slope and/or Level .

Auto-Print and Auto-Export of sample reports at the end of a run .

Storage for up to four 4L solvent bottles .

Integrated touch screen control .

3 rack positions dedicated to fractions .

Optimize your flow rate range with interchangeable pump heads 25, 50 and 100 mL/min . Up to 4 solvents

for B pump .

3 USB ports for easy download/upload methods and results, via flash drive . Easily connect your printer, keyboard and mouse .

Column holders for FLASH & Reverse Phase columns .

PLC 2020 Purification SystemLC in a Box!

Selection of up to 4 different solvents .

Interchangeable pump head design to accommodate wide flow rate and pressure ranges .

Tap Run on the main menu

Select Method and tap Run

Load and inject sample

A fully functioning purification system in a small, compact footprint, the PLC 2020 Personal Purification system was developed in response to the growing need for an easy-to-use, self contained system to handle a chemists purification needs on a more personal level . The PLC 2020 Personal Purification System is ideally suited for low-throughput, high pressure purification needs and is intended to support an individual or small group of chemists .

SpecificationsInjection Loop Volume

20 µL – 10 mL

Flow Rate1 – 100 mL/min

Maximum Pressure4060 psi (depending on pump head)

Solvent SelectionUp to 4 solvents for B pump

DetectionUV/VIS Dual Wavelength 190 –700 nm

NEW

! PLC 2020 Purification System

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To FLASH Waste

Mobile Phase

Detector

To FLASH

Waste

Mobile Phase

Detector

Accommodates any FLASH style cartridge as well as dry load configurations .

High pressure Reverse Phase columns .

Monitor the progress of the system without being next to it as run lights illuminate the racks during a run, turn off when a run is complete and FLASH if an error has occurred .

Running the System

Simple as:

Performs various techniques:FLASHNormal PhaseReverse Phase

Superior resolution to FLASHSave time—No need to purify the sample multiple times to achieve required purities .

FLASH

Product #: 21030000 (PLC 2020 Personal Purification System)

4Gilson, Inc . | 800-445-7661 | www .gilson .com3 Automated Liquid Handling | Product Guide

Application Run screen with real time fraction spectra

NEW

! LC/MS Purification System

Your time is valuable . The quality of your data is crucial . We understand you need simple, fast and flexible solutions that allow you to focus on your research not your equipment . That’s why Gilson has developed an LC/MS Purification System that is not only extremely easy-to-use and easy-to-maintain, but has one of the largest mass ranges on the market today .

LC/MS Purification SystemIt’s Your Chemistry... Simplified!

Trilution® LC SoftwareSimple and Specifically Designed for Preparative Purification

Complete and seamless integration into Trilution® LC Software allows for MS control in a single software package specifically designed for purification .

Intuitive, easy-to-use drag and drop method interface .

Unmatched fraction collection capabilities with Slope, Level, Time and Volume collection .

Real-time spectrum display of each fraction as they are collected means you wont need to wait for post-run analysis .

Your specificationsModular design means each component can be configured to your specifications .

Your own probeJust walk up and snap in to start, without worry of cross contamination in an open access, multi-user environment .

Your choice Choose to collect fractions using conditional logic based on any combination of UV, DAD, ELSD and up to seven MS Channels .

Multiple Mode Operation

Ability to monitor in Full Scan, Extracted Ion and SIM (Selected Ion Monitoring) simultaneously .

Range & Speed

Mass Range 50 – 3,000 amu

Scans Speeds up to 10,000 amu/sec

Source Features

The source features XYZ adjustments of probe positioning to optimize spray in relation to the capillary entrance for higher flows and/or sample concentrations .

Automation & Calibration

Integrated calibration vials for Auto-Tune allows for automated and effortless mass calibration .

MS Detector Features & BenefitsRugged & Simple

Rugged and simple to maintain source allows for cleaning and maintenance without breaking vacuum—limiting instrument down time and increasing productivity .

Quick-connect

Quick-connect feature allows each user to have their own probe, preventing contamination between users in open access environments .

Active Splitter

Active Splitter allows for large, setable split ratio to allow the system to operate with a large flow range and column conditions .

Mass Spec Single Quad Settings

Mass to beTransferred HPLC

Stream

Mass SpecStream

Mass Transferred

HPLC Stream

Mass Spec Stream

1

3

1

3

4 4

2 2

Aliquot Transfer

Mass to beTransferred HPLC

Stream

Mass SpecStream

Mass Transferred

HPLC Stream

Mass Spec Stream

1

3

1

3

4 4

2 2

Aliquot Fill

Separate make up pump dilutes and provides a constant, user setable flow rate to the MS independent of HPLC flow rate (0 .010 to 1 .5 mL/min) .

Split ratio is independent of changes in the HPLC flow

Product #: 3602671 (307 Pump)

Select conditional logic collection to trigger off both UV and MS

Enter target masses and adducts

Select scan range

RUN

Mass Based Purification

Simple as:

Product #: 18100003 (MS, PE FLEXAR SQ300 W/SRNG & DIV VLV)


The Gilson line of complete system solutions provide chromatography systems that range in flow rates from 0 .01 mL/min up to 200 mL/min and are capable of integrating the entire range of chromatography techniques from Reverse Phase to Ion Exchange .

Column ID

2 mm

4 .6 mm

10 mm

20 mm

30 mm

50 mm

100 mm

150 mm

SpecificationsPage

#

PLC 2020Flow Rate 1–100 mL/min

Injection Loop Volume 20 µL– 5 mL Sample Mass 300 mg/injection

3

LC/MS

Flow Rate 1–200 mL/min Injection Loop Volume 100 µL– 25 mL

Mass Range 50 – 3,000 amu Sample Mass 300 mg/injection

5

GX-281 Preparative HPLC System

Flow Rate 1 – 200 mL/min Injection Loop Volume 100 µL – 25 mL

Sample Mass 15 g/injection9

GX-281 Analytical to Semi-preparative HPLC System

Flow Rate 0 .15 – 30 mL/min Injection Loop Volume 2 µL – 2 mL

Sample Mass 300 mg/injection10

GX-271 Preparative HPLC System



GX-271 Analytical to Semi-preparative HPLC System

Flow Rate 0 .15 – 30 mL/min Injection Loop Volume 2 µL – 5 mL

Sample Mass 300 mg/injection12

845Zprep™ Semi-preparative to Preparative HPLC System



cLC™ Analytical to Preparative HPLC System


Sample Mass 15 g/injection FLASH chromatography

13

GX-271 Fraction Trapping HPLC System

Flow Rate 25 – 40 mL/min Injection Loop Volume up to 25 mL

Aspiration Flow Rate 5 mL/min for fraction loading

14

Gilson HPLC SystemsGilson high performance liquid chromatography (HPLC) systems provide complete solutions for all purification needs . Front-end liquid handlers and autosamplers are available in a range of bed capacities and optimized bench space footprints . All models accommodate innovative injection and fraction collection capabilities within a single platform . Trilution® LC Software completely controls the entire HPLC system, allowing focus to remain on the science rather than the software .

The GX-271 Liquid Handler, is a mid-size capacity and throughput instrument fulfilling numerous laboratory needs at an economical price . (see page 43)

The 215 Series Liquid Handlers, known as the workhorses of the industry, are available in single, multiple and variable spanning probe models . (see page 48)

Gilson GX-281 Liquid Handler is the premier front-end instrument featuring a large bed capacity and a wide array of components such as automatic reinjection of fractions using innovative GX Z-Injection technology . (see page 46)

Gilson VALVEMATE® II Valve Actuator offers multiple column selection availability featuring: fast switching times, high pressure valve control of 6 or 10-port, 2, 6 or 10-position and low pressure valve control of 6 or 8-positions . (see page 50)

Gilson accurate pumping solutions are available with multi-solvent selection, high pressure variable mixing and interchangeable pump heads . (see pages 20 – 24)

The Gilson variety of detection solutions offer excellent sensitivity and reproducibility including diode array (DAD), ultraviolet (UV) and/or visible (VIS) and evaporative light scattering (ELS) detection . (see page 25 – 27)

HPLC SystemsAnalytical, Semi-Prep, to Preparative Purification

Quick Comparison Chart


HPLC » System

s

7 Automated Liquid Handling | Product Guide

SpecificationsSample Loading

Capacity15 g/injection

Optimal Column Size4.6–100 mm

Maximum Injection Pressure3,000 psi (207 bar)

Injection Loop Volume

100 µL–25 mL

Flow Rate1–200 mL/min

Maximum Pump Pressure

3,040 psi (210 bar)

SpecificationsSample Loading Capacity

300 mg/injectionInjection Loop Volume

2 µL – 2 mL

Optimal Column Size2 – 20 mm

Flow RateH1: 150 µL/min – 15 mL/min H2: 30 µL/min – 30 mL/min


Maximum Pump PressureH1: 8,700 psi (600 bar)H2: 4,350 psi (300 bar)

GX-281 Preparative HPLC System GX-281 Analytical to Semi-preparative HPLC SystemThe GX-281 Preparative HPLC System

allows for injection, fraction collection and reinjection . The system is also ideal for liquid handling techniques such as sample mixing, dilutions and sample extract transfers .

The GX-281 is a multi-functional instrument that can perform injection, fraction collection and reinjection .

GX Direct Injection technology features an injection port that connects directly to the valve, minimizing volume associated with connective tubing (located behind the racks) .The GX Z-Injection Module

allows sample aspiration directly into the valve without a separate injection port, eliminating a source of potential carryover .

The prep-scale HPLC pumps feature low-dwell volumes which allow for fast, reproducible gradients . (see page 21)

The GX Prep Solvent System features a dynamic range of volumes and flow rates using syringeless technology within a solvent delivery pump capable of accessing five off-bed solvents .

The GX Z-Injection Module allows fast injections with low carryover and low maintenance by aspirating the sample directly into the valve .

The GX-281 features a Jet Wash Rinse station design that delivers individual streams of solvent for each separate rinse station to actively wash the outside of probes . Four rinse solvents are available for selection .

Separate injection and fraction collection paths reduce cross-contamination of fractions from sample injection . The cycle time of the injection is minimized by eliminating rinse steps which are required with single fluid path configurations .

Detection solutions are available for DAD, fixed or variable wavelength UV/VIS, UV, MS and ELS detection . (see page 25)

Semi-preparative HPLC pumps are offered to perform binary gradients . (see page 22)


HPLC » System

s


GX-271 Preparative HPLC System GX-271 Analytical to Semi-preparative HPLC SystemA single, mid-size instrument for injection and

fraction collection with separate fluid pathways to reduce cross-contamination of fractions .


15 g/injectionInjection Loop Volume

100 µL – 25 mL




Maximum Pump Pressure3,040 psi (210 bar)


300 mg/injectionInjection Loop Volume

2 µL – 5 mL


Flow RateH1: 150 µL/min – 15 mL/minH2: 30 µL/min – 30 mL/min

Maximum Injection Pressure

5,000 psi (345 bar)

Maximum Pump PressureH1: 8,700 psi (600 bar)H2: 4,350 psi (300 bar)

The GX Analytical Solvent System features innovative syringeless technology and access to five off-bed solvents .

The GX-271 is a mid-size liquid handler/fraction collector with a bench-saving footprint featuring separate fluid pathways that reduce cross-contamination of samples and fractions .

For confirmation or added detection capabilities of non-chromaphoric compounds configure an additional ELS detector with a multi-channel UV or DAD detector . (see page 25)

Binary gradients are achieved with analytical to semi-preparative HPLC pumps . (see page 22)

The low mount collection valve enables fraction collection to tubes via a separate fluid path from the injection .

High mount collection valve for collecting to small targets such as microplates and sealed vessels .

The GX Prep Solvent System uses syringeless technology and allows access to five off-bed solvents .

The VALVEMATE® II Valve Actuator can be incorporated for multiple column selection or column regeneration applications . (see page 50)

Mid-to-large collection capacity in a small footprint .

Binary gradient preparative HPLC pumps provide high-flow capabilities . (see page 21)

Modular detector options available . (see page 25)

The GX Direct Injection Module can utilize both 1/16" and 1/8" injection valves, allowing for a wide variety of sample volumes and loop options .


HPLC » System

s


845Zprep™ Semi-preparative to Preparative HPLC System

cLC™ Analytical to Preparative HPLC System

Gilson 845Zprep™ System features the first generation Z-Injection technology accommodating volumes and flow rates for semi-preparative to preparative applications .

Gilson cLC™ System is a complete chromatography system capable of automating the purification of compounds using both normal and reversed-phase columns . The cLC System has the ability to accommodate various modes of detection including DAD, UV/VIS, ELS and RI . Additional analytical components are also integrated onto the cLC System enabling automated on-line evaluation of the collected fractions for purity and recovery .




5 µL–25 mL

Optimal Column Size

4–100 mm

Flow Rate1–200 mL/min


5,000 psi (345 bar)


33X Pump: 3,040 psi32X Pump: 8,700 psi




3,000 psi (207 bar)

Optimal Column Size4.6–50 mm


3,040 psi (210 bar)


100 µL–10 mL

Carryover< 0.01%

Injection Variability< 1.5%


Mid-to-large bed capacity, accommodating both samples and fractions .

GX-271 Fraction Trapping SystemThe Gilson revolutionary Fraction Trapping System is unlike any other system on the market today . The Fraction Trapping System is ideally suited for processing fractions up to 20 mL in volume and concentrations up to 500 mg that have been purified on virtually any preparative HPLC system . Washing steps can be performed for removal of salts, buffers and mobile phase modifiers such as TFA that can hinder further LC/MS analysis . Fractions containing greater than even 50% water content can be processed and collected in 100% of organic solvent . This allows for fast dry down times at lower temperatures for samples that are heat sensitive . Dry down times of 12 – 24 hours can be reduced to less than 30 minutes .

SpecificationsFlow Rate50 mL/min

Wavelength190 – 700 nm


up to 25 mL

Aspiration Flow Rate

5 mL/min for fraction loading

DetectorVariable Dual Wavelength

Product #: 21120001 (GX-271 Fraction Trapping System)

Trapping Column Specifications

Trapping Columns Recommended Load Capacity

Recommended Flow Rate

10 mm ID x 150 mm 200 mg 25 mL/min

21 .2 mm x 150 mm 500 mg 40 mL/min

Process fractions in 8 to 12 minutes with volumes up to 20 mL and concentrations up to 500 mg .

Optional breakthrough detector and secondary fraction collector .

Steps involved in Fraction TrappingTRAP – Sequestration from fraction of desired solute(s) by a solid phase .

RINSE – Removal of buffers, modifiers, salts, etc . by appropriate rinsing of the solid phase and the ‘trapped’ solute(s) .

ELUTE – ‘Trapped’ solute(s) desorbed to produce a relatively small volume of ‘dry’ organic solvent solution of the desired solute .

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App: Standard FTLC Methods - Method: FTLC GX271 Prep Injector


HPLC » System

s


Gilson liquid handlers and injectors offer the broadest range of instrument capabilities on the market today . From small footprint injectors to larger footprint liquid handler/injector/fraction collectors, Gilson provides the ultimate front-end automation solutions for every HPLC system .

HPLC Liquid Handler/InjectorsFront-End Automation Solutions

Liquid Handler

Fraction Collection


Liquid Handling Accuracy

Injection Carryover

Page #

GX-281 Liquid Handler/ Injector 100 µL – 25 mL* 98%

(100 µL – 25 mL) < 0 .005% 16

GX-271 Preparative Liquid Handler/Injector

100 µL – 25 mL* 98% (50 µL – 25 mL) < 0 .05% 17

GX-271 Analytical Liquid Handler/Injector

2 µL – 2 mL 98% (10 µL – 25 mL) < 0 .05% 18

215 Liquid Handler/Injector 5 µL – 10 mL 99 .7%

(50 µL) < 0 .1% 19

* Larger loop volumes available upon request .

SpecificationsReproducibility

< 1% CV (total loop) < 3.5% (partial loop)


Injection Loop Volume100 µL – 25 mL


Accuracy98% (100 µL – 25 mL)

Injection Carryover< 0.005%

Dimensions (w x d x h)87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)

Specifically engineered for semi-preparative to preparative HPLC requirements, the GX-281 offers unmatched versatility and performance for front-end LC automation including revolutionary syringeless pumping technology .

Product #s: 261030 (GX-281 Liquid Handler)261031 (GX-281 Liquid Handler with bar code)261355 (GX Direct Injection Module, 1/8", prep)261354 (GX Direct Injection Module, 1/16", prep)261356 (GX Direct Injection Module,1/16", analytical SS)261357 (GX Direct Injection Module, 1/16", analytical PEEK)261353 (GX Z-Injection Module)

Immediate response aspiration and dispensing with the GX Prep Solvent System eliminates time spent waiting for valves to switch and syringes to fill .

Two unique and separate liquid pathways for injection and fraction collection provide a zero-contamination environment .

The GX Direct Injection Module features an injection port that connects directly to the injection valve for decreased dead volume and reduced maintenance .

Injection, Collection and Analysis

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GX-281 Liquid Handler/Injector


GX-281 Liquid Handler/Injector Bed Capacity

Tube Diameter

Sample Quantity

13 mm 57615 mm 57616 mm 45018 mm 42025 mm 18028 mm 162

Note: tubes up to 220 mm tall can be accommodated


HPLC » Liquid H

andlers/Injectors


Specifications GX-271 Preparative Liquid Handler/Injector

Flow Rate1 µL/min – 50 mL/min

Reproducibility< 1% CV (total loop)





Dimensions (w x d x h)59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

SpecificationsGX-271 Analytical Liquid Handler/Injector


Reproducibility< 1% CV (total loop)< 3.5% (partial loop)






The GX Direct Injection Module increases performance by incorporating a port that connects directly to the injection valve, reducing sample waste and maintenance .

Ethernet based communication provides better connectivity .

Product #s: 2614107 (GX-271 Preparative Liquid Handler)2614108 (GX-271 Analytical Liquid Handler)2614101 (GX-271 Liquid Handler without pump)261355 (GX Direct Injection Module, 1/8", prep)261354 (GX Direct Injection Module, 1/16", prep)261356 (GX Direct Injection Module,1/16", analytical SS)261357 (GX Direct Injection Module, 1/16", analytical PEEK)

Separate fraction collection flow paths prevent contamination from the injection process .

Available in an analytical or prep model, the solvent systems feature dynamic flow rate ranges and can access five off-bed solvents .

Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents .

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Recovery Data: Theophylline (5 mg/mL), Caffeine (5 mg/mL) and Phenol (3 mg/mL) Solution with 1 mL Injection Loop Volumes calculated from reinjection of collected Theophylline fractions (Zone 2)

Time (minutes)

% M

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Theophylline

Caffeine

Phenol

Series of Ten 20 µL Total Loop injections of Theophylline, Caffeine and Phenol

The GX-271 Liquid Handler/Injector can be configured for large injection loop volumes with minimal sample waste and carryover as well as to accommodate either the GX Prep or Analytical Solvent System which feature syringeless pumping technology .

The GX-271 Liquid Handler/Injector is the perfect blend of size, performance and capacity, consuming only 23” of linear bench space . Large-bore injection modules feature a continuous flow path design for preparative applications . Small-bore valves are available for analytical to semi-preparative applications with inert injection modules for bio-compatible applications .

GX-271 Analytical Liquid Handler/InjectorGX-271 Preparative Liquid Handler/Injector

Injection Recovery1 99 .86%2 96 .61%3 98 .22%4 96 .64%5 100 .10%6 98 .99%7 99 .57%8 99 .69%9 99 .81%

10 100 .36%Average Recovery 99.99%

Standard Deviation 0 .01

CV 1 .39%

PeakRetention

Time (minutes)

Area Count (µL V

min/100)

Standard Deviation CV

Theophylline 2 .8 14901136 .11 44928 .68 0 .30%

Caffeine 4 .2 12267689 .70 36503 .01 0 .30%Phenol 8 .4 13314605 .43 73087 .21 0 .55%

GX-271 Preparative and Analytical Liquid

Handler/Injector Bed Capacity

Tube Diameter

Sample Quantity

10 mm 540

13 mm 300

15 mm 220

16 mm 220

17 mm 220

18 mm 220

28 mm 70Note: tubes up to 200 mm tall can be accommodated


HPLC » Liquid H

andlers/Injectors


Column ID

Flow Rate Pressure

1 mm

2 mm

4 mm

10 mm

20 mm

>50 mm Page

#

333/334 Prep-scale HPLC Pumps

1 – 200 mL/min 3,040 psi (210 bar) 21

331/332 Semi-preparative HPLC Pumps

0 .5 – 50 mL/min 8,700 psi (600 bar) 22

321/322 Analytical to Semi-preparative HPLC Pumps

H1: 0 .15 – 15 mL/min H2: 0 .3 – 30 mL/min

H1: 8,700 psi (600 bar)

H2: 4,300 psi (300 bar)

22

30X Series HPLC Pumps

10 µL/min – 200 mL/min

8,700 psi (600 bar) 23

402 Syringe Pumps

1 µL/min – 120 mL/min

110 psi (8 bar) for 0 .1 – 10 mL syringes

40 psi (3 bar) for 25 mL syringe

23

Minipuls Evolution® Peristaltic Pump

standard: 0 .2 µL/min – 70 mL/min

high-flow: 1 – 340 mL/min

standard: 42 psi (3 bar)

high-flow: 29 psi (2 bar)

24

Minipuls 3 Peristaltic Pump

standard: (1, 2, 4 or 8 channels):

0 .3 µL/min – 30 mL/min

high-flow: (2 or 4 channels): 1 – 220 mL/min

standard: 72 psi (5 bar)

high-flow: 42 psi (3 bar)

24

The 215 Liquid Handler/Injector, a true and trusted laboratory workhorse for many years, performs injection, fraction collection and reinjection of collected fractions within a single platform .

215 Liquid Handler/Injector


liquid handling: 1.37% CV (50 μL)injection: < 0.9% (1 mL) (total loop)

Accuracyliquid handling: 99.7% (50 μL)


Injection Loop Volume5 μL – 10 mL

Dimensions (w x d x h)91 x 61 x 56 cm (36 x 24 x 22 in)

Product #s: 2510121 (215 Liquid Handler)251511 (819 Injection Module) 251551 (845Z Injection Module)251552 (845Zprep™ Injection Module)

This versatile, large bed liquid handler accommodates standard and custom racks to hold virtually any sample vessel .

Customize by adding accessories and components to meet laboratory needs (shown with 819 injection module) .

Gilson offers unsurpassed flexibility in HPLC pumping systems with an extensive product range and accommodations .

HPLC PumpsUnsurpassed Flexibility for All Types of Pumping Applications



Product #s: 38103331 (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump)38103341 (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump)

The entire preparative HPLC system is controlled with Trilution® LC Software, additionally the 333 model has an incorporated keypad for stand-alone operation .

Time (minutes)

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(mAU

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Gradient Linearity and Accuracy

Time (minutes)

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210180150120

906030

0-30

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Retention Time Repeatability

Series of Ten Injections

Methyl PB

Ethyl PB

Propyl PB

Average Retention

Time3 .44 4 .85 6 .08

Standard Deviation 0 .02 0 .02 0 .02

CV 0 .72% 0 .34% 0 .29%

Stackable to conserve bench space .

Capable of milligram-to-gram level mass-throughput per injection up to 15 g depending on column size and load capacity .

Selection of up to four solvents per pump for a total of eight solvents in a binary pumping system .

The flow rate can be doubled with additional parallel pumps to 400 mL/min .

SpecificationsFlow Rate

1 – 200 mL/min (3,040 psi)Accuracy

± 1% or 5.3 μL/min

Reproducibility< 0.5% or 2.7 μL/min RSD

Dimensions (w x d x h)333: 26 x 41 x 51 cm (10 x 16 x 20 in) 334: 26 x 41 x 39 cm (10 x 16 x 15 in)

Capable of fast separations with high-efficiency columns in normal and reversed-phase modes, Gilson 333 and 334 Pumps are the premier solvent delivery solutions for preparative chromatography . Choose the 333 master pump for isocratic applications or the 333/334 binary system for composition gradient with high pressure mixing (third pump for ternary) .

333/334 Prep-scale HPLC Pumps 331/332 Semi-preparative HPLC Pumps

321/322 Analytical to Semi-preparative HPLC Pumps

Product #s: 38103211 (321 Pump, H1)381032211 (left) (322 Pump, H1)38103212 (right) (321 Pump, H2)381032221 (322 Pump, H2)


0.5 – 50 mL/min (8,700 psi)Accuracy

± 2% or 13 μL/min

ReproducibilityH2: ± 0.7% or 7 μL/min

Dimensions (w x d x h)331: 26 x 41 x 51 cm (10 x 16 x 20 in)332: 26 x 41 x 39 cm (10 x 16 x 15 in)

Gilson 331 and 332 dual-piston pumps for semi-preparative and preparative chromatography feature a variable volume, dynamic mixer for optimized gradient performance .

The 321 and 322 multi-solvent pumps are designed for use with Gilson HPLC analytical and semi-preparative chromatography systems .

Product #s: 38103312 (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump) 38103322 (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump)

Smooth linear transposition from analytical to preparative HPLC methods using 4 to 20 mm ID columns packed with high-efficiency stationary phases .

Milligram-level sample mass-throughput per injection; 150 mg with a 20 mm bore column .

Solvent selection on each pump with optional 4-solvent valve for up to eight solvents .

High pressure Adjustable Volume Dynamic Mixer (AVDM™) enables fast gradient response times and homogeneous mixtures for analytical to semi-preparative applications .

While the pumps are controlled via trilution® LC, the 321 also incorporates a keypad that allows independent stand-alone gradient control .

A single 321 or 322 Pump is capable of binary gradients .

H1 pump heads are ideal for 2 to 10 mm ID columns .


H1: 0.15 – 15 mL/min (8,700 psi)H2: 0.3 – 30 mL/min (4,300 psi)

Accuracy± 2% or 13 μL/min

ReproducibilityH1: < 0.7% or 2 µL/min

H2: 4 µL/min

Dimensions (w x d x h)321: 26 x 41 x 51 cm (10 x 16 x 20 in)322: 26 x 41 x 39 cm (10 x 16 x 15 in)

trilution® LC Software provides control within a complete semi-preparative system including data processing and result reporting or stand-alone control via the keypad incorporated on the 331 Pump .

H2 pump heads are ideal for 4 to 20 mm ID columns .


HPLC » Pum

ps



305/306: 10 μL/min – 200 mL/min307: 10 μL/min – 10 mL/min

Accuracy< ± 1%

Reproducibility< 1%


Product #s: 360261 (305 Pump)3602661 (right) (306 Pump)3602671 (left) (307 Pump)

Tools are not required for pump head removal, allowing accessibility for maintenance . Piston seals, check valves and other parts can be replaced in a matter of minutes .

The 305 and 307 Pumps are programmable through the incorporated keypad .

The 307 model functions as an isocratic pump with a built-in manometric module .

Dynamic mixers ensure efficient, dependable gradient creation .

Product #s: F410511 (left) (402 Single Syringe Pump)F410512 (right) (402 Dual with Tee Syringe Pump)F410513 (402 Dual Syringe Pump)

The 402 Syringe Pumps assure accuracy in sample transfers, dilutions, reagent additions, mixing and more . They offer speed and reliability for repetitive liquid handling tasks .

402 Single, ideal for sampling injectors .

Available in three configurations:

402 Dual with Tee, provides rapid dilutions in both small and large volumes .

402 Dual, two-in-one accommodates simultaneous dispensing of multiple reagents and solvents .


0.8% (10 μL)Flow Rate

1 μL/min – 120 mL/min

Accuracy98.2% (10 μL)

Maximum Pressure 0.8 MPa (8 bar, 110 psi) for 0.1-to 10-mL syringes

0.3 MPa (3 bar, 40 psi) for 25-mL syringe

Dimensions (w x d x h)17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in)

An ideal addition to Gilson liquid handlers and injectors including the GX and 215 Series instruments .

The 306 model functions as a slave unit, controlled by either a 305 or trilution® LC Software .

Together with Gilson manometric modules and dynamic mixers, the 305, 306 and 307 Pumps offer a cost-effective, space saving and robust solution for isocratic or gradients of analytical to preparative HPLC .

30X Series HPLC Pumps Minipuls Evolution® Peristaltic Pump

Minipuls 3 Peristaltic Pump402 Syringe PumpsSpecifications

Flow Ratestandard: 0.3 μL/min – 30 mL/min

(72 psi, 5 bar)high-flow: 1 – 220 mL/min

(42 psi, 3 bar)

Head Speedcontinuous adjustment from 0 – 48 rpm by 0.01 rpm increments up to 9.99 rpm,

0.1 rpm increments above 10 rpm

Tubing IDstandard: 0.25 – 4 mm

high-flow: 2 – 8 mm

Dimensions (w x d x h)15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in)

Legendary robustness and ease of integration - The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems with control through an incorporated keypad or remotely with a separate device .

Product #: F155004 (Minipuls 3 Pump, single channel)

The Minipuls 3 accommodates wide flow rate ranges using either the standard or high-flow pump head . Pump heads are available with one, two, four or eight channels .

Multiple stainless steel rollers drive the pump to produce smoother, low pulse flows .

Specifications

Reproducibility≤ 0.1% on all channels

Tubing IDstandard: 0.25 – 4 mm

high-flow: 2 – 8 mm

Flow Ratestandard: 0.2 µL/min – 70 mL/min (42 psi, 3 bar)

high-flow: 1 – 340 mL/min (29 psi, 2 bar)

Head Speedcontinuous adjustment from 0 – 60 rpm by 0.01 rpm increments

up to 9.99 rpm, 0.1 rpm increments above 10 rpm

Dimensions (w x d x h)Module: 164 x 182 x 169 mm

MF1 pump head: 164 x 56 x 169 mmMF4 pump head: 164 x 92 x 169 mmHF2 pump head: 164 x 92 x 169 mm

Unmatched flexibility and performance for any liquid transfer application - The Minipuls Evolution® is designed for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations .

Gilson offers the only peristaltic pump that allows up to 16 separate channel cartridges to be changed while the pump is running with a simple push on the cartridge for placement and removal .

Optimized six roller drive limits pulsation .

Product #: F110701(Minipuls Evolution® Pump)

Enhanced reliability and ease of operation are possible because the pump’s speed stability is maintained independent of normal temperature variations from 0˚ to 40˚C . This makes the pump ideal for biological perfusion applications . (Note: not intended for direct connection to human subjects.)


HPLC » Pum

ps


Gilson offers a complete line of HPLC detection solutions that are ideal for analytical, semi-preparative and preparative HPLC applications .

HPLC DetectorsVersatile, Rugged & Accurate Detection Solutions

Diode Array (DAD)

Ultra- violet (UV)

Visible (VIS)

Evaporative Light

Scattering (ELS)

Mass Spec.(MS) Range

Spectral Band Width Channels

Page #

171/172 Diode Array Detectors

190 – 720

or 190 – 610 nm

— 8 26

15X Series UV/VIS Detectors 190 – 700 nm 9 nm dual &

single 26

112 UV Detector 254 or

280 nm0 .2 or 12 nm single 27

prepELS™ II Detector — — single 27

MS Detector 50 – 3,000

amu — 7 6

Gilson 171 and 172 Diode Array Detectors deliver rugged and accurate detection for both preparative and analytical HPLC . Detection is done with a fiber optic light path that can be configured in a fixed or remote flow cell design .

Product #s: 18100001 (171 Diode Array Detector)18100002 (172 Diode Array Detector)

With a remote flow cell, the power of the detector is located directly at the outlet of the column, which yields virtually no peak broadening .

An interchangeable flow cell design allows for both the 171 and 172 Detectors to accommodate analytical and semi-preparative to preparative applications .

SpecificationsPixels

171: 256172: 512

S/N Ratio171: < 3 x 10-4 AU172: < 2 x 10-4 AU

Wavelength171: 190 – 720 nm172: 190 – 610 nm

Pixel Distance171: ~2.2 nm/pixel172: ~0.8 nm/pixel

Wavelength Accuracy< 0.5 nm

Wavelength Repeatability< 0.1 nm

Baseline Drift5 x 10-4 AU/h

Wavelength Temperature Drift< 0.005 nm/°C


171/172 Diode Array Detectors

15X Series UV/VIS DetectorsGilson 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, rugged detection with an interchangeable flow cell design to accommodate analytical to preparative HPLC applications .

(see page 72 for Flow Cell Selection Chart)

Product #s: 10105311 (right) (151 UV/VIS Detector, with flow cell/accessory kit)10105411 (152 UV/VIS Detector, with flow cell/accessory kit)10105511 (155 UV/VIS Detector, with flow cell/accessory kit)10105611 (left) (156 UV/VIS Detector, with flow cell/accessory kit)

SpecificationsLamp

UV: deuterium, 750 hrsVIS: tungsten/halogen, 500 hrs

Setting Accuracy± 2 nm

Wavelength190 – 700 nm

Sensitivity0.001 – 2 AU

Setting Precision± 0.2 nm

Drift3 x 10 - 4 AU/hr

Spectral Band Width

9 nm


The following modes are available to specify detection conditions and basic operating parameters:

Single wavelength with dual sensitivities (151 and 152)

Dual wavelengths (155 and 156)

Scan wavelengths (155 and 156)

Choose either the Gilson trilution® LC Software or the stand-alone keypad control on the 151 and 155 Detectors .

Up to ten methods can be stored .



HPLC » D

etectors


SpecificationsLight Source

670 nm laser diode, < 5 mWEvaporative Zone Temperature

ambient – 120˚C

Gas Requirement65 ± 5 psi Nitrogen or Argon

Liquid Flow Rate0.2 – 5 mL/min

Splitting packages available to accommodate system flow rates up to 75 mL/min

Nebulization Temperature0˚ – 70˚C

Dimensions25 x 46 x 29 cm (9.8 x 18 x 11.5 in)

The 112 UV Detector is a rugged, economical detector for analytical, preparative HPLC and GPC clean-up .

Product #: 0310511(112 UV Detector, with lamp and 11 μL flow cell/accessory kit)

SpecificationsLamp

mercury (standard): 254 nm, 2,000 hrs phosphor-coated mercury: 280 nm, 500 hrs

Spectral Band Width254 nm: 0.2280 nm: 12

Flow Cell Sensitivity< 4 x 10 – 4 AU for flow step

of 0.5 – 2 mL/min (Methanol)

Drift3 x 10 – 4

AU/hr

Wavelength254 or 280 nm

Dimensions (w x d x h)32 x 32 x 13 cm

(12.6 x 12.6 x 5.2 in)

Accessible lamp/filter assemblies for selecting wavelengths .

Fixed wavelength design for increased sensitivity and durability .

10 mm path length analytical flow cell provides excellent sensitivity ranging from 0 .001 to 1 AUFS (Absorbance Units, Full Scale) .

Reduced operating and setup time with a single step baseline function .

Event-marking function conveniently marks injection point or fraction collector advance on either the recorder or data system .

A front panel dial allows selection of sensitivity level to adjust the absorbency of a sample .

Product #s: 18100100 (prepELS™ II Detector, 120V)18100200 (prepELS II Detector, 240V)

Time (minutes)

ELSDUV

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125

100

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50

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The prepELS™ II Detector is ideal for obtaining the purest fractions with the ability to detect virtually any analytes with or without a chromophore, including compounds that have poor UV absorption such as sugars, carbohydrates, lipids and triglycerides .

112 UV Detector

prepELS™ II DetectorThe Gilson evaporative light scattering (ELS) detector, prepELS™ II, provides capabilities on the analytical-scale with the ability to detect as low as nanograms of analyte while a unique filtering technology removes baseline noise without distressing peak height or shape .

Gilson offers advanced solutions for fraction collection with a complete line of stand-alone and software-controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between .

HPLC Fraction CollectorsIdeal Solutions for Small to Large Capacity Collection

Capacity

Large Small Flow Rate Microplate Tubes 1 L Bottles Page #

prepFC™Fraction Collector 200 mL/min 29

FC 203B Fraction Collector 20 mL/min 30

FC 204 Fraction Collector 20 or 200

mL/min 30



Choose the compact FC 203B Fraction Collector for small capacity collection or the FC 204 for larger volumes . Collect fractions by time, drop or peak, accommodating all LC applications . Both models accept a wide variety of collection vessels including tubes, vials and microplates .

In peak mode, collectors monitor a detector signal and identify peaks using either an adaptive-slope algorithm or a specified mV threshold .

The fraction collector utilizes multi-column adapters for collecting up to 18 separate channels .

Its compact size fits in most fume hoods .

Product #s: 171011 (right) (FC 203B)171011DV (FC 203B, 3-way valve)171041 (left) (FC 204)171043 (FC 204, prep valve)

Up to ten programmable time windows can be added for collecting only samples of interest, while discarding the column’s void volume, areas of non-interest and equilibration volumes .

The 3-way diverter valve prevents contamination of collected fractions (standard on FC 204 and optional on FC 203B) . Contact closure inputs are available

to remotely start, advance or stop fraction collection .

SpecificationsDimensions (w x d x h)

FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in)

FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in)

Valve Switching Speed100 ms

Liquid Contact MaterialPTFE and 316 stainless steel

Max Flow RateFC 203B: 20 mL/min

FC 204: 20 or 200 mL/min

Valve Dead VolumeFC 203B/FC 204: 64 μL internal volume;

3.5 μL dead volume from common port to normally closed portFC 204: 114 μL internal volume;

6 μL dead volume from common port to normally closed port

Stand-alone instruments are controlled with an incorporated keypad or through the Gilson Trilution® LC Software .

Gilson prepFC™ Fraction Collector is a large capacity, compact footprint fraction collector ideal for semi-preparative to preparative HPLC applications . The prepFC features an adjustable tray that accommodates various vessel heights, volumes and sizes .

prepFC™ Fraction Collector FC 203B/FC 204 Fraction CollectorsSpecifications


Valve Dead Volume114 μL internal volume; 6 μL dead volume from common port to normally closed port

Liquid Contact MaterialPTFE and 316 stainless steel

Flow Rate200 mL/min

Valve Switching Speed< 20 ms

Collection Modesslope, level, time or volume

Pocket PC control for stand-alone operation allows the prepFC™ to be added to any HPLC system capable of sending contact closures .

High-flow fraction collection valve .

Product #s: 17110101 (prepFC™ Fraction Collector - Ethernet)171071 (prepFC™ Fraction Collector)

prepFC Control Software:Remote start and tube advance .

Up to ten programmable collection windows .

Active chromatogram display with start and stop fraction collection lines .

Customizable collection patterns and rack addition .

Increased X/Y speed optimizes fraction collection .

prepFC™ Bed Capacity

Sample Quantity Size Vessel Rack

320 13 x 100 mm (9 mL) tubes (4) Code 343 Racks




54 28 x 57 mm (25 mL)

scintillation vials (2) Code 204 Racks

54 30 x 115 mm (50 mL)

conical bottom tubes

(2) Code 222 Racks

18 48 x 113 mm (125 mL) bottles (2) Code 211 Racks

11 (500 mL) bottles (1) Code 91 Rack8 (1 L) bottle (1) Code 92 Rack

240 12 x 32 mm (2 mL) vials (4) Code 330 Racks

56 (20 mL) scintillation vials (4) Code 333 Racks




Increase bed capacity by configuring up to four fraction collectors in a single system (provides over 1,000 fractions) .


HPLC » Fraction Collectors


Gilson offers powerful software to complement robust instruments . With accommodating features, drag-and-drop functionality and numerous supplied tasks, Gilson software packages allow effortless yet complete control of instruments, allowing you to focus on the science rather than the software .

Control SoftwareSoftware Designed by Scientists for Scientists

HPLC Systems SPE SystemsGPC Clean-up

Systems Liquid HandlersPage

#

Trilution® LC Software

32

Trilution® LH Software

33

Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and other countries . Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and/or other countries .

Fraction Collection Capabilities:Collection modes: slope, level, time or volume .

Conditional logic fraction collection .

Graphical fraction collection optimization .

Slope collection settings: front, tail and APEX .

Auto sample list generation .

Open Access Module: Administrator control and permission-setting capabilities .

Walk-up user access .

Minimal training required .

Conforms to the application needs rather than compromising the application in order to fit the software .

Configuration and Virtual Lab Screen

Computer RecommendationProcessor

Intel® Pentium® 4 (2.8 GHz);

Intel® Core™ 2 Duo (1.86 GHz)

Fixed Drive120 GB, available

space: 4 GB (6 GB at install)

Video Card1280 x 1024 with24 bit True Color

RAM2 GB

Additional DriveDVD/CD-RW

I/O Ports1-Serial port

1-Ethernet port

Operating SystemMicrosoft® Windows® XP Professional SP2 or SP3

Product #s: 210631R21 (Trilution® LC Media)21063130 (Trilution® LC Lifetime License)21063130L5 (Trilution® LC Multi-user Lifetime License - 5)21063130L10 (Trilution® LC Multi-user Lifetime License - 10)21063131 (Trilution® LC 1 year License)Note: Requires Oracle® Database 10g Express Edition

Method Builder Control Screen

Fraction Collection Settings Page

Gilson has redefined purification with the HPLC system control software, Trilution® LC . This liquid chromatography software, specifically designed for purification, allows for complete control to achieve the challenging results that applications require .

Trilution® LC Software


Application Run Screen


Control Software


Main Menu

Method in Method Builder Screen

Over 70 Gilson-supplied tasks enable quick and efficient method programming .

Quick and effortless instrument setup, bed layout and method creation .

Sample list with spreadsheet functionality and graphical run simulation .

Run methods in either batch or sequential mode to optimize performance .

Manual control for performing routine tasks such as priming instruments and troubleshooting .

Graphical custom task creation and cartridge monitoring .

Product #s: 210630R20 (Trilution® LH Media)21063020 (Trilution® LH Lifetime License)21063021 (Trilution® LH 1 Year License) Note: Requires Oracle® Database 10g Express Edition

Computer Recommendation

ProcessorIntel® Pentium® 4

(2.8 GHz)

Fixed Drive80 GB, available

space: 4 GB (6 GB at install)

Video Card1280 x 1024 with 24 bit True Color

RAM1 GB

Additional DriveDVD/CD-RW

Serial Ports2

Operating SystemMicrosoft® Windows® XP Professional SP2 or later

Microsoft® Windows® 2000 SP4

Liquid Handling

Disposable Tips

Solid Phase Extraction

Tweaks Auxiliary

Gilson Trilution® LH Software, designed by scientists for scientists, is a complete control solution for liquid handling and solid phase extraction applications . trilution LH features a user-friendly graphical interface with icon-based drag-and-drop functionality minimizing the learning curve and optimizing application performance .

Trilution® LH Software

Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements .

The Gilson gel permeation chromatography (GPC) clean-up systems are an ideal solution for post-extraction clean-up of interfering substances from environmental samples, food products and animal tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS . Failure to remove these interfering compounds can cause poor analytical results, shorten column life and increase analytical downtime .

GPC Clean-up SystemsIdeal Solutions for Your Post-extraction Clean-up Requirements

Fraction Collection

Multiple Fractions

Solid Phase Extraction Control

Page #

Automated GX-271 GPC Clean-up System

0 .25 – 450 mL (optional)

trilution® lC Software 35

GX-271 GPC/SPE Clean-up System

0 .25 – 450 mL (optional)

trilution® Software 36

Manual GPC Clean-up System

0 .25 – <1,000 mL (optional)

307 Pump Keypad 37


Simulation Mode in Application Run Screen

SS Dispense Task Property Page


SpecificationsAutomated GPC Systems

Flow Rate0.01 – 10 mL/min



Maximum Pressure4,000 psi (276 bar)

Compatible with various GPC columns from low pressure glass to high pressure stainless steel .

Product #s: 21110000 (Automated GX-271 GPC Clean-up System, with 112 UV Detector)21110001 (Automated GX-271 GPC Clean-up System)

The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample extractions with large fraction collection capacity into an assortment of vessels .

Automated GPC Systems Bed Capacity

Sample Quantity Description GPC Collection Rack Product #36 38 x 200 mm (170 mL) collection tubes Code 320 Rack 26044005085 25 x 200 mm (70 mL) collection tubes Code 321 Rack 26044005113 61 x 137 mm (250 mL) Boston Round bottles Code 322 Rack 2604400528 Labconco RapidVap (450 mL) flat-bottom evaporation flasks Code 323 Rack 260440053

20 Caliper TurboVap II (200 mL) evaporation tubes Code 324 Rack 2604400548 Labconco RapidVap (450 mL) evaporation flasks, with stem Code 325 Rack 260440055

50 Caliper TurboVap II (50 mL) evaporation tubes Code 326 Rack 26044005632 Labconco RapidVap (100 mL) flat-bottom evaporation flasks Code 327 Rack 26044005723 49 x 114 mm (125 mL) Boston Round bottles Code 328 Rack 26044005812 Pyrex 4100 (125 mL) flat-bottom flasks for rotary evaporators Code 329 Rack 2604400598 Pyrex (250 mL) flat-bottom flasks for rotary evaporators Code 331 Rack 260440060

Sample Quantity Description GPC Sample Rack Product #80 13 x 100 mm (8 mL) tubes Code 343 Rack 26044002544 16 x 100 mm (12 mL) tubes Code 346 Rack 260440049

The Gilson Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in addition to GPC for sample extract purification; a multi-purpose sample preparation system .

Positive Pressure elution increases reproducibility and precision .

Sample Capacity SPE: 60 (3 mL), 45 (6 mL)GPC: 80

Mobile Rack technology enables programmed switching from drain and collect positions to completely automate the entire SPE process from condition to load, wash and elute .

The GX Direct Injection Module reduces sample waste and carryover, which prevents the need to overfill the sample loop .

Automated GX-271 GPC Clean-up System GX-271 GPC/SPE Clean-up System


GPC Clean-up System

s


Automate solid phase extraction (SPE) processes with the complete instrument line from Gilson . From 96-well extraction plates to 1, 3 or 6 mL cartridges, Gilson automated SPE solutions meet any application need .

The Gilson Manual GPC Clean-up System is a simple and economical solution within a small footprint requiring minimal bench space .

Manual GPC Clean-up SystemSpecifications

Flow Rate0.01 – 10 mL/min


Maximum Fractions10 separate

Maximum Pressure4,000 psi (276 bar)


Collection of multiple fractions from the same sample extract is done with the VALVEMATE® II Valve Actuator .

Integrate a 112 UV Detector for EPA method compatibility .

Product #s: 21110002 (Manual GPC Clean-up System, with 112 UV Detector)21110003 (Manual GPC Clean-up System)

Keypad provides simple setup and control of the system for time-based fraction collection .

Manual injection valve coordinates injection time .

CO

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G S

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ANALYTE INTERFERENCES

Liquid Handler

Multiple Collection

Positive Pressure Elution

Cartridge Sizes 1 mL 3 mL 6 mL

Page #

GX-271 ASPEC™ 39

GX-274 ASPEC™ 40

Quad-Z 215 Liquid Handler for SPE

41


Solid Phase Extraction SystemsWhatever Your Requirements for Throughput, Gilson SPE Products Provide a Solution

A Typical 4-step SPE Method


SpecificationsGX-27X ASPEC™ Series

Cartridge Capacity108 (1 mL), 60 (3 mL), 45 (6 mL)

Positive Pressure Modes syringe or gas cylinder/source

Accuracy98% (100 μL – 25 mL)

Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)

Choose between the 406 Single or, for more flexibility, the 406 Dual Syringe Pump for both large and small volume capabilities . Both pumps have built-in pressure sensing capabilities for detecting clogged fluid lines and monitoring cartridge pressure on each fluid line .

The GX ASPEC™ Series provides rugged and reliable automation of SPE applications utilizing 1, 3 or 6 mL cartridges .

The GX-271 ASPEC™ is truly the solid solution for low-to mid-throughput sample clean-up and extraction .

Product #s: 2614007 (left) (GX-271 ASPEC™, with 406 Single Syringe Pump)2614008 (right) (GX-271 ASPEC™, with 406 Dual Syringe Pump)261355 (GX Direct Injection Module, 1/8", prep)261354 (GX Direct Injection Module, 1/16", prep)261356 (GX Direct Injection Module,1/16", analytical SS)261357 (GX Direct Injection Module, 1/16", analytical PEEK)

Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision in comparison to vacuum based instruments .

On-bed access to four 500 mL or 700 mL reservoirs .

Utilizes Mobile Rack technology to completely automate the SPE process by increasing capacity and flexibility while performing both SPE and liquid handling .

AutomatedSolid Phase Extraction Cartridges

Integrate a GX Direct Injection Module for on-line injection onto HPLC or LC/MS following SPE clean-up .

Optional safety shield available .

GX-271 ASPEC™ GX-274 ASPEC™

Mobile Rack technology enables the entire SPE process to be automated from the initial condition to the final elution and transfer while allowing access to cartridges and compounds of interest . The linear rail system permits automated method development and method optimization in which all or selected steps are collected and evaluated .

On-bed transfer ports allow access to two additional large volume solvent reservoirs; incorporating a VALVEMATE® II provides access to ten off-bed solvents .

Process four SPE cartridges simultaneously for increased throughput .

Built-in pressure sensing on each fluid line monitors cartridge pressure and responds to blockage by activating an error handling method .

Product #: 2614010 (GX-274 ASPEC™,with two 406 Dual Syringe Pumps)

Positive Pressure elution increases reproducibility and precision due to process consistency .

The GX-274 ASPEC™ is an ideal solution for mid to high-throughput sample clean-up and extraction .


Solid Phase Extraction Systems


Gilson Quad-Z 215 Liquid Handler is a flexible and robust system that performs liquid handling tasks, on-bed filtration and Positive Pressure SPE .

This liquid handler accommodates various racks maximizing flexibility for high-throughput applications .

Quad-Z 215 Liquid Handler for SPE

Code 300 Series RackMobile Rack technology enables automated switching from drain and collect positions to accomplish the entire SPE process from condition to load, wash and elute, including selective evaluation of individual steps for method development and optimization available through N+1 movement into individual collection tubes .

SpecificationsCartridge Capacity

288 (1 mL), 144 (3 mL), 60 (6 mL)Accuracy

98% (100 μL – 25 mL)


Using Code 300 Series Racks with Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision .

Four independent probes with independent variable spacing ranging from 9 to 18 mm for access to various vessel sizes .

Product #: 25101413 (Quad-Z 215 Liquid Handler for SPE )

Multiple Probes

Disposable Tips Reproducibility Accuracy

Page #

GX-271 Preparative / Analytical Liquid Handler

< 1% (100 µL – 25 mL)

98% (50 μL – 25 mL)

98% (10 μL – 5 mL)

43

GX-274 Liquid Handler 0 .8%

(10 μL)98 .2% (10 μL) 45

GX-281 Liquid Handler

< 1% (100 μL +)

98% (100 µL – 25 mL) 46

Quad-Z 215 Liquid Handler 2 .1%

(1 µL of DMSO)104%

(1 µL of DMSO) 47

Quad-Z 215 Liquid Handler with Disposable Tips 4 .3%

(1 µL of DMSO)113%

(1 µL of DMSO) 47

215 Liquid Handler 1 .37% (50 µL of DMSO)

99 .7% (50 µL of DMSO) 48

223 Sample Changer

0 .8% (10 µL)

98 .2% (10 µL) 48

Gilson liquid handlers are industry renowned for rugged, reliable performance at an economical price . Whether the application requires a single probe, multiple probes, variable spacing or disposable tips, Gilson liquid handling solutions meet all needs .

Liquid HandlersUnmatched Solutions to Meet Your Liquid Handling Requirements



Gilson GX Solvent System, available in an analytical or prep model, features a dynamic range for volumes and flow rates and can access five off-bed solvents .

Product #s: 2614107 (GX-271 Preparative Liquid Handler)2614108 (GX-271 Analytical Liquid Handler)2614101 (GX-271 Liquid Handler without pump)

Consumes only 23" of linear bench space .

Flexible bed accommodates five racks and virtually any tube, vial or microplate .

Ethernet based communication provides better connectivity .

The GX-271 Liquid Handler offers a medium capacity with a small footprint . The GX-271 models are economical and robust liquid handlers for reformatting, reagent dispensing and performing serial dilutions . Their modular design allows for future expansion by adding injection and fraction collection capabilities .

(see GX-271 Liquid Handler/Injector page 17)

SpecificationsGX-271 Preparative Liquid Handler


Flow Rate1 µL – 50 mL/min


SpecificationsGX-271 Analytical Liquid Handler


Flow Rate1 µL – 5 mL/min


GX Analytical Solvent System Transfers

Volume CV Accuracy1 µL 11 .98% ± 300 nL

10 µL 4 .5% ± 1 µL1 mL 0 .18% ± 20 µL5 mL 0 .2% ± 100 µL

GX Prep Solvent System Transfers

Volume CV Accuracy

50 µL 1 .25% ± 2 µL

100 µL 1 .18% ± 4 µL500 µL 0 .48% ± 7 µL1 mL 0 .14% ± 5 µL5 mL 0 .09% ± 16 µL

10 mL 0 .07% ± 105 µL25 mL 0 .02% ± 46 µL

GX-271 Preparative and Analytical Liquid Handler Bed Capacity

Tube Diameter

Sample Quantity

10 mm 54013 mm 30015 mm 22016 mm 22017 mm 22018 mm 22028 mm 70

Note: tubes up to 200 mm can be accommodated

The GX-271 Liquid Handler is available without a pump or GX Solvent System, allowing the instrument to be configured with a 402 Syringe Pump .

GX-271 Analytical Liquid Handler GX-271 Preparative Liquid Handler

Liquid Level Detection allows for detecting a variety of solvents as low as 20 µL in a 384-well microplate .

Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents .

GX Prep Solvent System Transfers

% CV

Volume

GX Analytical Solvent System Transfers

% CV

Volume


Liquid Handlers


GX-274 Liquid HandlerThe GX-274 Liquid Handler is a mid-throughput, four probe instrument capable of processing up to four samples (at 18 mm fixed spacing) in parallel, increasing the efficiency of liquid handling applications . The GX-274 Liquid Handler provides an economical option with a small footprint for achieving increased throughput in a variety of applications .

The GX-274 is ideal for bulk reagent dispensing when used in conjunction with a Minipuls Evolution® or Minipuls 3 Peristaltic Pump or for tube-to-plate transfers using 402 Syringe Pumps .

SpecificationsAccuracy

98.2% (10 µL)Reproducibility< 0.8% (10 µL +)


Access four on-bed solvent reservoirs without consuming a rack position .

Product #s: 2614102 (GX-274 Liquid Handler)F410513 (402 Dual Syringe Pump)

Flexible rack system accommodates tubes, vials and microplates .

Optional flowing rinse station with solvent ports provides active probe rinsing from off-bed solvents .

A VALVEMATE® II can be incorporated enabling access to ten additional off-bed solvents .

25 mL Transfers at 25 mL/min.

Transfers

Volu

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25

20

15

10

5

0 0 2 4 6 8 10 12

10 mL Transfers at 25 mL/min.

Mea

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d Vo

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10

7.5

5

2.5

0

Dispenses0 20 40 60 80 100 120 140 160 180

5 µL Dispense

The GX-281 Liquid Handler is the premier liquid handling instrument from Gilson . The GX-281 offers unmatched versatility and performance for liquid handling operations including tube-to-plate transfers, serial dilutions, compound reconstitution and sample filtering through the use of Code 300 Series Racks and more . The efficient speed of the GX-281 allows applications to be completed quickly and reliably . The GX Solvent Systems offer a wide range of volumes and flow rates via patented syringeless technology .

Direct request aspirating and dispensing eliminates the time spent waiting for syringes to fill .

Optional integrated bar code scanner verifies rack types and positions .

Access five off-bed solvent reservoirs for bulk reagent dispensing and multiple solvent rinsing protocols .

Product #s: 261030 (GX-281 Liquid Handler)261031 (GX-281 Liquid Handler, with bar code scanner)

GX-281 Liquid Handler Bed Capacity

Tube Diameter

Sample Quantity

13 mm 57615 mm 57616 mm 45018 mm 42025 mm 18028 mm 162

Note: tubes up to 220 mm can be accommodated

SpecificationsReproducibility < 1% (100 µL +)


Dimensions (w x d x h)87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)


GX-281 Liquid Handler


Liquid Handlers


The Quad-Z 215 Liquid Handler was designed based on the robust Gilson 215 Liquid Handler . This instrument accommodates numerous racks for maximum flexibility of sample transfer between tubes, vials and microplates .

Product #s: 25101413 (Quad-Z Liquid Handler)2506163 (Quad-Z 215 Adapter Kit for Disposable Tips)2515154 (849 Multiple Injection Module)F410513 (402 Dual Syringe Pump)

Integrating two 402 Dual Syringe Pumps provides four independently controlled syringes from 100 μL to 25 mL .

For access to the various vessel sizes, the four independent probe variable spacing ranges from 9 to 18 mm .

Flexible configuration enables use of fixed probes and 10 μL, 200 μL and 1 mL disposable tips .

SpecificationsDimensions (w x d x h)

91 x 61 x 61 cm (36 x 24 x 24 in)

Accuracy104% (1 μL of DMSO)

Reproducibilityliquid handling: 2.1% (1 μL of DMSO)

injection: < 2.0% (500 μL syringe, partial loop); < 5.0% (500 μL syringe, total loop)


Injection Loop Volume5 μL – 5 mL

Disposable Tips Option

Accuracy113% (1 μL of DMSO)

Reproducibility0.8% (10 μL +); 4.3% (1 μL of DMSO)

Quad-Z 215 Liquid Handler 215 Liquid Handler

223 Sample ChangerThe 223 Sample Changer is a programmable sampler for automated sample preparation and transfer . It provides a cost-effective solution for protocols such as serial dilutions, additions, sampling into vials, timed reactions and tube-to-tube transfers .

The 223 Sample Changer is ideal for automated transfer of samples in analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP) .

Cross-contamination is minimized by adjusting rinse volumes and using liquid level sensing .

Product #: 191015 (223 Sample Changer)


1.37% (50 µL of DMSO)

Carryover< 1 ppb

Accuracy99.7% (50 µL of DMSO)


Flexible open bed design allows for a variety of rack options including tubes, vials, microplates and Peltier control .

Product #s: 2510121 (215 Liquid Handler)2510191 (215 Liquid Handler without pump)

The 215 Liquid Handler is a laboratory tested workhorse for performing dilutions, aliquoting solutions, mixing, plate reformatting, reconstituting of samples and more . The 215 Liquid Handler has a punch strength capable of piercing thick or thin film septa and microplate mats .


0.8% (10 μL)Accuracy

98.2% (10 μL)

Dimensions (w x d x h)53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in)

Optional flow-through rinse station, available on the left and/or right of the support bar, reduces cross-contamination .

Accommodates a wide variety of probes as well as standard and custom racks .

Choose between an optional 402 Syringe Pump, Minipuls Evolution® or Minipuls 3 Peristaltic Pump to add aspirating and dispensing flexibility .

Quad-Z 215 Liquid Handler with Disposable TipsThe Gilson Quad-Z 215 Liquid Handler can be configured with a disposable tips option, a requirement in molecular biology and biochemistry applications . Disposable tips prevent cross-contamination, which saves time by eliminating rinsing steps . This system includes on-bed storage of tips, as well as a chute for collection of used tips .To see the complete line of Gilson disposable tips see page 57.


Liquid Handlers


Switching Valve Mixing Racks Page #

VALVEMATE® II Valve Actuator 50

Orbital Shaker 51

RackCount™ System 51

818 AutoMix 52

Complementing robust and flexible instruments, Gilson accessories optimize instrumentation to fit application requirements for the ultimate solution .

AccessoriesOptimize Your Instrumentation

High Pressure Valves:Analytical 2-position, 6-port, stainless steel (0 .016" ID ports) .

Analytical 2-position, 10-port, stainless steel (0 .016" ID ports) .

Preparative 2-position, 6-port, stainless steel (0 .030" ID ports) .

Preparative 2-position, 10-port, stainless steel (0 .030" ID ports) .

Preparative 2-position, 6-port, PPS (0 .030" ID ports) .

Preparative 2-position, 10-port, PPS (0 .030" ID ports) .

Multi-position, 6-port, stainless steel (0 .016" ID ports) .


Multi-position, 6-port, PEEK (0 .016" ID ports) .


Multi-position, 10-port, PEEK (0 .016" ID ports) .

Product #: 331052AB (VALVEMATE® II Valve Actuator)

The VALVEMATE® II Valve Actuator automates HPLC and LC techniques by increasing the system flexibility to save time, increase throughput and extend column life . It is designed to facilitate both solvent selection and column switching . The VALVEMATE II increases flexibility of HPLC systems allowing for multiple valve and multiple column configurations .

Specifications


Valve Switching Speed< 180 ms port-to-port (depending on valve)

Contact closure control for home and switch valve position .

Multi-directional keypad control with valve position LED display .

Fast valve switching times minimize pressure spikes and carryover .

Low Pressure Valves:Multi-position, 6-port, PPS (0 .030" ID ports) .

Multi-position, 6-port, PPS (0 .060" ID ports) .



6 Column Plumbing Schematic

VALVEMATE® II Valve Actuator


Table of Contents


Accessories


Gilson RackCount™ System, numbered overlays that fit securely on the top of racks, provides a quick and effortless bed location reference for specific samples or collected fractions on a Gilson liquid handler .

The Gilson Orbital Shaker is specifically designed to satisfy liquid handling needs for on-line or off-line vortexing and mixing . It is a fully functional, stand-alone instrument equipped with a single-stepper motor .

The Orbital Shaker can be placed on the bed of the GX-281 or 215 Series instruments using the optional mounting plate kits .

SpecificationsCircular Rotational Speed

20 – 720 rpm

Rack Capacity(1) Code 200 Series Rack

Dimensions (w x d x h)11.8 x 31.6 x 4.7 cm

(4.65 x 12.44 x 1.85 in)

RackCount™ overlays can be used with new rack purchases or added to existing Gilson Code 200 Series Racks .

They have sequentially numbered fraction collection positions for five complete rack positions and one position for sample locations .

The numbering patterns are solvent resistant and compatible with most normal and reversed-phase solvents .

Product #: 251711 (Orbital Shaker)

Orbital Shaker 818 AutoMix

RackCount™ System Racks – Standard & Custom

Designed for Gilson 215 Series instruments, the 818 AutoMix provides fully automated, software-driven mixing and sampling . Typical applications that use the AutoMix include mixing whole blood samples, immiscible liquids and more .

Product #: 251520 (818 AutoMix)

Mix an entire rack of samples with oscillating mixing .

SpecificationsMixing Speed/Accuracy

10 rpm: ± 0.5 rpm20 rpm: ± 1 rpm

Rack Capacity(1) Code 200 Series Rack

Dimensions (w x d x h)22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in)

Gilson offers standard and custom racks to meet the needs of any and all applications . A variety of vessels can be accommodated including tubes, vials, microplates and microchips .

Table of Contents


Accessories


Complete your equipment with:Safely store and charge your pipette at the same time with the PIPETMAN M Stand Adapter .Designed to fit in the Gilson Carrousel™ Stand and Single™ pipette holder, the adapter allows you to simplify your daily work and organize your bench .

PIPETMAN® M Model

PIPETMAN® DIAMOND Tips* Product # Volume

(µL)

Maximum Permissible ErrorsGilson ISO8655

Systematic Error (µL)

Random Error (µL)


Random Error (µL)

P10MD10

DL10DF10

DFL10F81001

Min : 0 .5 1 5Max : 10

± 0 .04± 0 .025± 0 .060± 0 .080

≤ 0 .013≤ 0 .012≤ 0 .020≤ 0 .025

± 0 .12± 0 .12± 0 .12± 0 .12

≤ 0 .08≤ 0 .08≤ 0 .08≤ 0 .08

P20MD200DF30

DF200F81002

Min : 2 10Max : 20

± 0 .075± 0 .100± 0 .150

≤ 0 .025≤ 0 .035≤ 0 .050

± 0 .2± 0 .2± 0 .2

≤ 0 .1≤ 0 .1≤ 0 .1

P200MD200D300

DF200DF300

F81003Min : 20 100Max : 200

± 0 .40± 0 .80± 1 .00

≤ 0 .15≤ 0 .22≤ 0 .26

± 1 .6± 1 .6± 1 .6

≤ 0 .6≤ 0 .6≤ 0 .6

P1000MD1000D1200

DF1000DF1200

F81004Min : 100 500Max : 1,000

± 2 .5± 3 .5± 5 .0

≤ 0 .4≤ 0 .7≤ 1 .1

± 8 .0± 8 .0± 8 .0

≤ 3 .0≤ 3 .0≤ 3 .0

* Recommended tip for establishing maximum permissible errors (ISO 8655 requirement) . A large range of suitable PIPETMAN DIAMOND Tips is available .

Lightweight and extremely comfortable, the new PIPETMAN® M from Gilson is a fully motorized pipette that requires virtually zero pipetting forces to aspirate and dispense samples . Simply turn the push-button to adjust settings and then click!

Use it like a Mechanical PipetteKeep your technique consistent by using only one button to adjust the volume and to pipette .

PIPETMAN® M is so easy to use that you will have the feeling that you are using a manual pipette .

The standard for accuracy and precision – MotorizedDesigned with both experienced and inexperienced users in mind, PIPETMAN M pipettes are intuitive and require no learning curve to use .

Motorized piston virtually eliminates pipetting variability among users .

Adjustable piston speed feature allows users to pipette denser samples with accuracy and confidence .

GLP FeaturesLockable volume .

Color-coded push-button corresponds to matching TIPACK™ .

Service alert reminders .

Expanded FlexibilityFour essential pipetting modes – reverse, mix, repetitive and pipet .

Volume range of 0 .5 µL to 1,000 µL in only four models .

Longer use between charge cyclesPIPETMAN M’s Lithium-ion battery has been designed to ensure 900 pipetting cycles at maximum speed (up to seven 96 well plates) between charge cycles .

PIPETMAN M charges 80% of its full battery capacity in less than an hour and takes only three hours to fully charge .

Optimized BalanceLightweight, but strong materials and components make PIPETMAN M one of the lightest motorized pipettes ever .

0,0Competitor A

Volum

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Force

(N.cm

)W

eight

(g)

PIPETMAN Neo Competitor B

Model (200 or 300 µL)

Competitor C PIPETMAN M

1,0

2,0

3,0

4,0

5,0

0

Competitor A

Competitor B

Competitor C

PIPETMAN Neo

Competitor D

Competitor E

Competitor F

Competitor G

PIPETMAN M P200M

50

100

150

200

250

Electronic PipettesModel

(200 µL or 300 µL)Mechanical Pipettes

Trusted resultsBest results guaranteed with a performance exceeding ISO recommendations . PIPETMAN® M helps to avoid variable results due to user inexperience, thanks to motorized pipetting . This special feature guarantees highly reproducible results, especially for small volumes .

PIPETMAN M pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory, upon request .

Improved ComfortPIPETMAN® M contributes to lowering Repetitive Strain Injuries (RSI):

Thanks to its motor, PIPETMAN® M offers you pipetting with minimized pipetting forces .

Low ejection and volume setting forces allow you to work longer without fatigue .

As Simple as pipetman® with Enhanced Ergonomic Features & New Motorized User-friendly Functionality

Product #: F807023(Stand Adapter)


Manual Liquid H

andling


PIPETMAN® Neo Models

PIPETMAN® DIAMOND Tips* Product # Volume

(µL)

Gilson Maximum Permissible Errors

ISO 8655 Maximum Permissible Errors


Random Error (µL)


Random Error (µL)

P2ND10

DL10DF10

DFL10F144561

0 .20 .512

± 0 .024± 0 .025± 0 .025± 0 .030

≤ 0 .012≤ 0 .012≤ 0 .012≤ 0 .014

± 0 .08± 0 .08± 0 .08± 0 .08

≤ 0 .04 ≤ 0 .04≤ 0 .04≤ 0 .04

P10N**D10

DL10F144562

15

10

± 0 .025± 0 .075± 0 .100

≤ 0 .012≤ 0 .030≤ 0 .040

± 0 .12± 0 .12± 0 .12

≤ 0 .08≤ 0 .08≤ 0 .08

P20ND200DF30

F1445632

1020

± 0 .10± 0 .10± 0 .20

≤ 0 .030≤ 0 .050≤ 0 .060

± 0 .2± 0 .2± 0 .2

≤ 0 .1≤ 0 .1≤ 0 .1

P100NDF100D200

F1445641050

100

± 0 .35± 0 .40± 0 .80

≤ 0 .10≤ 0 .12≤ 0 .15

± 0 .8± 0 .8± 0 .8

≤ 0 .3≤ 0 .3≤ 0 .3

P200ND200D300

DF200DF300

F14456520

100200

± 0 .50± 0 .80± 1 .60

≤ 0 .20≤ 0 .25≤ 0 .30

± 1 .6± 1 .6± 1 .6

≤ 0 .6≤ 0 .6≤ 0 .6

P1000ND1000D1200

DF1000DF12000

F144566100500

1,000

± 3 .0± 4 .0± 8 .0

≤ 0 .6≤ 1 .0≤ 1 .5

± 8 .0± 8 .0± 8 .0

≤ 3 .0≤ 3 .0≤ 3 .0

* Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable PIPETMAN DIAMOND Tips is available. ** P10N model can be used from 0.5 µL.

Trusted resultsBest results guaranteed with a performance exceeding ISO 8655 specifications . PIPETMAN® Neo’s specifications are more stringent than those required to be ISO 8655 compliant .

Wide useful volume rangeSpecifications provided at 10%, 50% and 100% of the nominal volume, as recommended by the ISO8655 standard .

New clip ejector for an easier dismantling and greater tip fitting

The new clip ejector results in easier daily operation and even more robust system over time .

Thanks to this improvement, the ejector clips securely in place without risk of it slipping over time .

PIPETMAN® Neo pipettes are available with a calibration certificate issued by an ISO 17025 accredited laboratory, on request .

clip ejector

PIPETMAN® Neo is an air-displacement pipette that addresses the growing susceptibility of pipette users to repetitive strain injuries .

PIPETMAN Neo exhibits a distinct enhancement in comfort and a wide useful volume-range with the same legendary robustness, accuracy and precision as PIPETMAN Classic .

Six single-channel models cover a range of volumes from 0 .2 µL to 1,000 µL .

Up to 50% reduction of pipetting forcesNewly engineered springs are wound to provide a significant reduction of pipetting and purge forces .

Up to 50% reduction of pipetting and purge forces on certain models .

Color-coded push-buttons Color-coded push-buttons facilitate identification of the different models and corresponding quality PIPETMAN® DIAMOND Tips .

Helps differentiate PIPETMAN Neo pipettes from PIPETMAN Classic models .

45

40

35

30

25

20

15

10

5

0

0 5 10 15 20 25

Pipe

ttin

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ce (N

)

Piston stroke (mm)

Dispensing Purge

P200

P200N

The Pipetting Standard with Lower Spring & Ejection Forces

Each Neo Starter Kit contains three PIPETMAN Neo pipettes plus boxes of certified-quality PIPETMAN Tips . We’ve also included three Jimmy™ microtube openers, three Single™ pipette holders, a “Two Minute Inspection” poster and a Gilson Guide to Pipetting .

Save money when compared to pipettes and accessories purchased separately .

pipetman® Neo Starter Kits Enhanced comfort. Maximum savings.

Expanded flexibilityModels P2N and P10N incorporate dual position tip ejectors . Allows use of D10 (short) and DL10 (long) tips – a plus for protection and precision when using microtubes .

PIPETMAN® Neo Starter Kits

Model Product # PIPETMAN Neo Models

Gilson Tips (racks of 96 Tips) Accessories

PIPETMAN Neo Starter Kit F167700

P20NP200N

P1000ND200 and D100

3 Jimmy™ microtube openers3 Single pipette holders

1 Gilson Guide to Pipetting

PIPETMAN Neo PCR Starter Kit F167600

P10NP100N

P1000N

Sterile filter tipsDFL10ST, DF100ST

and DF1000ST

3 Jimmy™ microtube openers3 Single pipette holders

1 Gilson Guide to Pipetting Gilson maximum permissible errors are guaranteed only when PIPETMAN pipettes are used with the recommended Gilson PIPETMAN Tips .

Short tips

Dual position tip ejector

Long tips


Manual Liquid H

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Sterilized tips Free of detectable amounts of RNase, DNase, DNA, RNA, Proteases and trace metals .

No contamination. Free of biological contaminants with an SAL of 10-6 .

All packs are wrapped in medical device quality plastic that provides a complete shield against contamination .

Quality Tips for Researchers Who Care About the Integrity of Their Results Did you know…Tips contribute up to 50% to the performance of your pipette!

100

95

90

85

80

75

70

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

Filte

r e�e

ctive

ness

(% )

Aerosol diameter (µm)

Gilson A B C DFilter Tip brands:

PIPETMAN® Filter Tips Provide an Effective Filter Against Aerosols0.018

0.016

0.014

0.012

0.01

0.008

0.006

0.004

0.002

0-0.125 -0.1 -0.075 -0.05 -0.025 0 0.025

A B C D E F G H IDL-10 Gilson speci�cationsTip brands:

Prec

ision

(µl)

10 measurements of 1 µL at 21°c using a PIPETMAN P10 with various tip brands.Accuracy (µl)

PIPETMAN® Tips Deliver Maximum Accuracy

Date of sterilization and red sticker sterilization guarantee!

Ergonomic Tip FittingPIPETMAN® Tips and the PIPETMAN Tip holders are designed to work in harmony, which means a low tip-mounting force and a perfect seal every time . Easy to mount . Easy to eject .

A Perfect PointImperfections in a tip’s geometry can lead to excess droplets being released into your results . Gilson prides itself on its perfect point and smooth surface, ensuring that all of your sample is dispensed every time .

PackagingOur tips are available in convenient rack packaging in our Tower Pack™ refill system or Tipack™ racked boxes for economic and efficient handling .

We also offer bagged bulk quantities in our EcoPack™ and EasyPack™ configurations .

Available in sterilized or autoclavable packages .

Color-coded packaging for GLP compliance .

Naturally hydrophobic and low-retentionSome companies add lubricants to make their tips hydrophobic . Gilson PIPETMAN® Tips are made with the highest grade, 100% pure virgin polypropylene, an inert plastic characterized by low retention and naturally hydrophobic properties so no chemicals leach into your expensive samples .

Graduated volume markersVolume graduations make it easy to visually check the volume aspirated .

Prevent sample and pipette contamination with PIPETMAN® Filter TipsThe Gilson high-density polyethylene filter blocks small particles down to 1 µm from entering your pipette, providing an effective barrier against aerosol contamination .

Fully traceable from the manufacturer to your benchThe Gilson Trident guarantees quality . With identification and batch numbers on all PIPETMAN tip packaging, you can be assured that quality is our number one priority .


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PIPETMAN® Tips (Contd.)

Tower Pack™: refill system for Gilson Tips

10 racks of 96 tips

Tower Pack™: refill system for Gilson Tips

10 racks of 96 tipsEcoPack™ EasyPack™ Empty Racks

Autoclavable Product Number

-Ray sterilizedProduct Number

Box of 10,000 tips (10 bags of 1,000) Product Number

Box of 1,000 tips (5 bags of 200 or 1 bag of 1,000)

Product Number

Set of 2For manual loading only. Not compatible with Tower Pack

refill system. Product Number

F167101 F167201 F161630 F161631 F161630/1 F161791

F167102 F167202 F161450 F161451 F161450/1 F161393

F167103 F167203 F161930 F161931 F161930/1 F161391

F167105 F167205 F161730 F161731 F161730/1 F161737

F167104 (7 racks

of 96 tips)

F167204 (7 racks of

96 tips) F161670 F161671

F161670/1 F171502

Part Item Number Universal Reload Box F167100 Required for use of Tower Pack tips. Must be purchased separately.

F161100 F161111 F161104

* F161571 (3 bags of 334)

F161360(1 rack)

* F161210 (4 bags of 50)

F161220(1 rack)

PIPETMAN Filter Tips

Tip Model

Pipette Models

Volume Range Tip Length (mm)

-Ray sterilizedProduct Number

DF10ST P10M, P2, P10, P2N, P10N,U2, U10

0 .1 - 10 µL 30 mm

F171103

DFL10ST P10M, P2, P10, P20, P2N, P10N, P20N, P8x20N, P12x20N, F2 to F20,

U2, U10, U20, U8x20, U12x20, C10, C8x10, C12x10

0 .1 - 10 µL 45 mm

F171203

DF30ST P20M, P20, P20N, P8x20N, P12x20N F2 to F20, U20, U8x20, U12x20

2 - 30 µL 51 mm

F171303

DF100ST P200M, P100, P200, P100N, P200N, P8x200N, P12x200N F25 to F200, U100,

C100, C8x100, C12x100

10 - 100 µL 51 mm

F171403

DF200ST P200M, P200, P200N, P8x200, P8x200N, P12x200N

F200, U100, U200, U8x300, U12x300,

C300, C8x300, C12x300

20 - 200 µL 51 mm

F171503

DF300ST P200, P200N, P8x200N, P12x200N

F200, U200, U8x300, U12x300, C300, C8x300, C12x300

20 - 300 µL 51 mm

F171603

DF1000ST P1000M, P1000, P1000N, F250 to F1000, U1000,

C1200

100 -1,000 µL 51 mm

F171703

DF1200ST P1000M, P1000, P1000N F250 to F1000 U1000, C1200

100 - 1200 µL 66 mm

F171803

PIPETMAN® Tips

Tip Model

Pipette Models

Tipack™ Gilson Tips: 10 Tipacks of 96 racked tips

M=PIPETMAN M P=PIPETMAN P

PxN=PIPETMAN Neo U=PIPETMAN Ultra

C=PIPETMAN Concept F=Fixed PIPETMAN

Volume Range Tip Length (mm)

Autoclavable Product Number

-Ray sterilized Product Number

D10 P10M, P2, P10, P2N, P10N U2, U10, C10

0 .1 - 10 µL 30 mm

F171100 F171101

DL10 (Longer tip)

P10M, P2, P10, P20, P2N, P10N, P20N, P8x20N, P12x20N

U2, U10, U20, U8x20, U12x20, C10, C8x10, C12x10

0 .1 - 20 µL 45 mm

F171200 F171201

D200 P20M, P200M, P20 to P200, P8x200, P20N to P200N, P8x20N,

P12x20N, P8x200N, P12x200N F2 to F200,

U20 to U200, U8x20, U12x20, U8x300, U12x300, C100, C300, C8x100,

C12x100, C8x300, C12x300

2 - 200 µL 51 mm

F171300 F171301

D300 P200M, P200, P200N, P8x200N, P12x200N U200, U8x300, U12x300, C300, C8x300, C12x300

20 - 300 µL 51 mm

F171400 F161735

D1000 P1000M, P1000, P1000N, F250 to F1000, U1000, C1200

100 -1,000 µL 66 mm

F171500 F171501

D1200 P1000M, P1000, P1000N, F250 to F1000, U1000, C1200

100 - 1200 µL 84 mm

F161112 F161113

D5000 P5000, U5000, C5000 1 - 5 mL 10 .4 cm

F161370 (12 Tipacks of 50 tips)

*

D10 mL P10 mL, U10 mL, C10 mL 1 - 10 mL 16 cm

F161230 (8 Tipacks of 30 tips)

*

*Not applicable to this model .

New Tipacks now available!

Quality Tips for Quality Pipettes

DF300 & DF1200 PIPETMAN® DIAMOND Tips

Specifically designed for PIPETMAN® Concept C300 and C1200, DF300 and DF1200 filter tips also perfectly fit all 200 µL and1,000 µL PIPETMAN models, respectively .

With more space between the liquid and the filter when used with these models, they prevent any risk of having the liquid coming into contact with the filter while still offering high accuracy and precision .

Product #: F171503(D200ST)


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Maximum Control16 programming possibilities for pipetting patterns (rows, columns, etc .), pipette types and plate types .

Timer function enables hands-free operation .

Optional foot pedal available for hands free control .

Get on track, keep on track, as you pipette flawlessly with the help of your new personal laboratory assistant . TRACKMAN™ simplifies your work, improves your productivity and helps eliminate errors and cross-contamination .

Versatile PerformanceDuo-plate tracker lights the way to your pipetting so you avoid losing track of where you need to start or where you left off .

Can be used with both 96 and 384 well microplates and single and multichannel pipettes .

Easily switch from using one size plate to another on the same instrument .

16 Programming Possibilities for a Variety of ApplicationsDifferent pipetting patterns, (individually through rows, columns or other configurations .), different pipette types and vessel types!

Complete your equipment with:Expand the functionality of your Trackman™ with the versatile microtube–holder to transfer liquids from one microtube to another (0 .5 mL, 1 .5 mL and 2 mL) .

Enjoy hands-free operating and individual speed control, when you equip your Trackman with the Gilson optional foot pedal .

Always OperationalLong lasting battery allows cordless operation for up to eight hours .

Portable slim design takes up small footprint on your benchtop .

The TRACKMAN™ prevents errors, saving you time & money!

Contemporary DesignLight-weight and ergonomic .

Use with 0 .5, 1 .5 and 2 .0 mL tubes with the optional tube holder accessory .

Optimal positioning .

4

3

2

1

Intuitive Software Interface

1. Plate types (96 or 384 wells or microtubes)

2. Indicator of well or tube position

3. Pipette types (1, 8 or 12 channels)

4. Timer function or manual4

3

2

1

Guiding You to Success

Product #: F70261(Pedal for TRACKMAN®)

Product #: F70105 (Microtube-holder)

Product #: F70301(TRACKMAN™)


Manual Liquid H

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Gilson offers many instruments and component to fit your OEM needs . Please contact oem@gilson .com for more information .

Gilson Knowledge Center

2011 Customer SPE & PurificationEducation Program

2011 Customer Pipette & TipEducation Program

OEM InformationSupport for Original Equipment ManufacturersGilson factory to factory direct channel supports the unique design and production requirements of original equipment instrument and assay manufacturers . This allows the OEM partner to focus on their area of expertise while receiving world class reliability, flexibility, documentation and support from Gilson for the automated liquid handling portion of the system .

Gilson OEM channel provides reliable liquid handling instruments and components that integrate with your OEM product, moving your system quickly and reliably to market or adding automated capabilities to existing manual systems . Gilson provides flexible OEM terms and delivery, software development support and on-going training .

FlexibilityWide array of modular design .

Flexible instrument control designed for 3rd party software .

Extensive mechanical, electronic and software engineering and prototyping capabilities to modify existing products to meet OEM requirements .

ReliabilityStringent specifications and procedures and 50 years of liquid handling experience give reliable, quality solutions .

Long term partnerships are the goals of Gilson .

QualityRegulatory compliance on standard products .

Extended OEM warranty .

Service, support and validation .

ISO 9001 Certified .

Customer Education & TrainingThe Gilson Knowledge Center assists today’s scientists by offering flexible and innovative education and training courses that focus on operating Gilson products and systems for your applications . Choose from our free web seminars, local workshops, classroom training and web training!

Learn more at www.gilson.com

Specifications OEM GX-Syringe Module

Resolution 38,400 steps

Stroke60 mm

Precision/Accuracy 0.15 %RSD at 100% stroke length0.8% accuracy at 500 µL dispense

from 500 µL syringe

Wetted Materials FEP, PTFE, PEEK, Ekonol®,

glass, ceramic

Communication RS-485, up to 32 pumps

addressable

Valves Selection, multiport valves

Maximum Pressure 0.8 MPa (8 bar, 110 psi) for 0.1-to 10-mL syringes

0.3 MPa (3 bar, 40 psi) for 25-mL syringe

Specifications GX-271 OEM Liquid Handler

XYZ work zone 389 mm X, 294 mm Y, 200 mm Z

Probe positioningAccuracy: ±0.75 mm in X,Y and Z

Repeatability: 0.20 mm in X,Y and Z

Liquid Handling External pump connected via

calibrated tubing. Gilson syringe, peristaltic or continuous and reversible

flow solvent pumps available.

Input/Output Two inputs (contact closure control &

TTL), two relay outputs, two accy ports (RS-485 and 24v) one safety stop input,

3-way fraction valve control on Z-arm

Communication OptionsRS-232, RS-485, Ethernet

Punch strength 44 Newtons maximum at 125 mm/sec53 Newtons maximum at 80 mm/sec

OEM Instruments & Component Examples


Resources


Gilson Publications

Gilson Guide to SPE AutomationYears of experience with Solid Phase Extraction (SPE) and automation of this very important clean-up and sample preparation technique has provided us the opportunity to compile a large amount of tips, best practices, suggestions and optimization/development strategies . Organized into a spiral bound guide, this compiled information is an essential ‘tool book’ for any chemist performing SPE .

Inquiries for ordering the Gilson Guide to SPE Automation can be made to your local Gilson Representative, Gilson Distributor or to training@gilson .com .

Gilson HPLC Troubleshooting GuideTroubleshooting High Pressure Liquid Chromatography (HPLC) systems can be straightforward, despite the multiple components . With our guide that contains helpful forms and checklists, troubleshooting Gilson HPLC systems is simpler than ever! Included in this guide are real-life examples using HPLC systems with real problems and solutions organized into a spiral bound guide to eliminate problems that may be related to software, hardware, column, solvent, or any combination of these . Simple tests to prove purification and reduce or eliminate carryover are included!

Inquiries for ordering the Gilson HPLC Troubleshooting Guide can be made to your local Gilson Representative, Gilson Distributor or to training@gilson .com .

Gilson Guide to PipettingGet tips and best practices from the experts and inventor of the adjustable volume pipette with our guide to pipetting . Applications, sample solutions and ergonomics are just a few very important topics discussed . This essential laboratory guide compiles years of experience and use of pipettes for different applications in one compact book . Utilize this as a reference guide to assist you with pipetting with the highest degree of accuracy and repeatability in any situation .

Inquiries for ordering the Gilson Guide to Pipetting can be made to your local Gilson Representative, Gilson Distributor or to training@gilson .com .

Technical Notes Handbooks

Gilson Quarterly Application Notes HandbooksThe complimentary quarterly Gilson Application Notes Handbook allows you to page through scientific advances using Gilson automation and Gilson pipettes in a number of different markets from other chemists and laboratories who have published applications . We welcome you to submit your application to us for publishing in our quarterly handbooks .

Published applications offer everyone a chance to learn about the recent scientific highlights and new technologies . Attend our complimentary G .E .T . LIVE Web Seminars to learn about more scientific advances with industry leading applications and technologies from TODAY! Gilson is the host for our guest speakers, who lead the industry with their innovative methods and techniques . Contact training@gilson .com or visit www.gilson.com to learn more about our Web Seminars - they are FREE!

How to Order Your Handbook:

Inquiries for ordering the Gilson Trilution® LH v2 .0 Technical Notes Handbook or Trilution® LC v2 .1 Technical Notes Handbook can be made to your local Gilson Representative, Gilson Distributor or to training@gilson .com .

Product #: GKCSPEGUIDE (Gilson Guide to SPE Automation)

Product #: GKCTLH21 (Gilson Trilution® LC v2 .1 Technical Notes Handbook)

Product #: GKCTLC20 (Gilson Trilution® LH v2 .0 Technical Notes Handbook)

Product #: GKCHPLCGUIDE (HPLC Troubleshooting Guide)

Product #: LT303409-01 (Gilson Guide to Pipetting)

Receive ‘how to’ information on the most popular functions in the Gilson Trilution® LH and LC software packages by following step-by-step from our Technical Notes Handbooks . These handbooks guide you through how to perform some of the most important and essential functions that optimize your use of the Gilson Trilution software . Attend a Gilson quarterly Trilution® G .E .T . LIVE Web Course to be entered for a drawing to receive a complimentary Technical Note Handbook .

Trilution® LH v2.0 and Trilution® LC v2.1 Trilution® LH v2.0 Technical Notes Handbook Chapters:

Liquid Level Detection & Liquid Level Following .

Virtual Pumping Systems .

Creating Custom Racks .

Resources .

Trilution® LC v2.1 Technical Notes Handbook Chapters:

Fraction Collection Fundamentals .

Methods for Verifying Fraction Recovery in Preparative HPLC Systems .

Resources .

Contact training@gilson .com or visit www.gilson.com to learn more about our Web Courses!


Resources


Gilson products support the pharmaceutical, food & beverage, environmental and clinical/forensic markets with a primary focus on purifying samples via preparative HPLC, automated SPE or through GPC clean-up .

Preservation and Quantification of Hormones in Surface Water

Using the Gilson GX-271 ASPEC™ System

The contamination of aquatic environments with hormones can disrupt the reproductive and developmental function of aquatic organisms such as fish (Jensen, K .M . et al ., 2006; Orlando, E .F . et al ., 2004) . One source of these hormones is the natural and synthetic hormones that are present in manure from large-scale livestock operations . Approximately 130 billion pounds of manure are produced annually in the United States (USEPA, 2000) . The hormones that are present in manure and in crop fields that have been amended with manure can be readily transported to surface and shallow ground water with events such as rain or snowmelt . There is a great deal of interest in studying the distribution and fate of these hormones in surface water . This application note describes the use of different preservatives (sodium azide, hydrochloric acid and sulfuric acid) to inhibit the degradation of hormones in samples of surface water runoff from cattle manure-amended fields during storage at 4° C . The Gilson GX-271 ASPEC™ System was used for this application .

An Automated Method for the Fractionation of Extractable Petroleum Hydrocarbons (EPH) from Water and Soil Extracts

Using the Gilson GX-274 ASPEC System

Crude and refined petroleum products contain a complex mixture of aliphatic and aromatic hydrocarbons as well as a variety of other compounds . The concentration of these different hydrocarbons will vary in different products such as crude oil, refined products and other residual oil products . Many of these hydrocarbons have been shown to pose a risk to human health or to aquatic life . Leaking underground storage tanks are a common source of groundwater and soil contamination . It is

important to determine the types of hydrocarbons that may be present in contaminated soil and water . Government agencies and other regulatory bodies have developed several methods for determining the types of aliphatic and aromatic hydrocarbons that may be found in contaminated soil or water . The Gilson GX-274 ASPEC was used for this application .

Evaluation of Several Columns and Solvents for Post-Extraction Gel Permeation Chromatography (GPC) Clean-up of Fish Tissue Prior to PCB Analysis

Using the Gilson GPC Clean-up System

The determination of PCBs, as well as other environmental contaminants, in fish tissue requires extensive sample cleanup prior to analysis by gas chromatography with an electron capture detector (GC-ECD) . The high fat content of fish tissue can cause buildup of nonvolatile materials on the GC injection port and the analytical column resulting in poor analytical results and high instrument maintenance costs . Gel Permeation Chromatography (GPC) is a size-exclusion clean-up procedure and it is a highly effective method for the removal of high molecular interferences such as lipids, proteins, pigments and cellular components from animal tissue . The Gilson GPC Clean-up System was used for this application .

An Automated Method for the Selective Solid Phase Extraction of Zearalenone from Wheat Using Molecularly Imprinted Polymers


Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)-ß-resorcyclic acid lactone] is a mycotoxin produced as a secondary metabolite of various Fusarium fungi . ZON is also known as RAL, F-2 toxin or ZAR . Zearalenone (ZON) has been detected in a variety of cereal products such as wheat, maize, barley, oats, rice and sorghum as well as beer that has been produced with contaminated grains . ZON can be excreted in cow’s milk after lactating cows are fed ZON in high doses (JECFA, 2000) . Exposure to ZON, in animals such as pigs and dairy cattle, has been known to cause estrogenic effects including infertility, reduced serum testosterone levels and sperm counts, reduced incidence of pregnancy and changes in progesterone levels . In addition, ZON can delay the breeding process and cost the producer significant economic and physical losses . Molecularly Imprinted Polymers (MIPs) have been demonstrated to be very effective tools for the selective extraction of an analyte from a complex matrix such as a food product (Haginaka, 2009; Wei et al ., 2007) . The Gilson GX-271 ASPEC™ System was used for this application .

Analysis of Sucralose in Water by Liquid Chromatography/ Time-of-Flight Mass Spectrometry (LC/TOF-MS) after Automated Solid Phase Extraction


Non-nutritive sweeteners are steadily increasing in importance with increased awareness of diabetes and its special dietary requirements and with more consumers becoming concerned about obesity and dental caries . Some of the most widely used non-nutritive sweeteners include saccharin, aspartame and sucralose . Sucralose is a water soluble tri-chlorinated disaccharide that is 600 times sweeter than sugar . Sucralose has a low human toxicity and is not metabolized by the human body . There has been a great deal of interest in monitoring levels of sucralose in water samples (Dye, 2007; Brorström-Lundén, 2007; Mead et al ., 2009) .Recently, some methods employing liquid chromatography-mass spectrometry (LC/MS) have been published for the analysis of sucralose (Loos et al ., 2009) and other artificial sweeteners in water samples (Scheurer et al ., 2009) . The Gilson GX-271 ASPEC System was used for this application .

An Automated Liquid Handling Strategy for Complex Matrices Based on Sample Viscosity


Performing manual liquid handling procedures in the laboratory is commonplace . When a laboratory faces an increase in sample volumes and throughput, it is often necessary to automate these liquid handling procedures . Automation of the liquid handling steps should improve day-to-day reproducibility of results . The viscosity of a liquid, defined as the resistance to a fluid to shear motion, can be especially challenging when developing an automated liquid handling protocol . Liquids with a higher or lower viscosity compared to water may require a different set of parameters to optimize the method . This note describes an automation strategy for the scientist who is transitioning from a manual liquid method to an automated method that is based on the viscosity of the liquid matrix . The Gilson GX-271 ASPEC System was used for this application .

Environmental Applications

Food & Beverage Applications Gilson Applications

GX-271 ASPEC™

GX-271 ASPEC™

GX-274 ASPEC™

GPC Clean-up


Applications


Clinical & Forensic Pharmaceutical

Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling


Glycans consist of polymers of monosaccharides joined together by glycosidic linkages . Glycans are complex in structure and can be either branched or linear . Glycans are commonly attached to proteins to form glycoproteins . N-linked glycans (N-glycans) are the most common glycans bound to proteins in eukaryotic cells (Apweiler, R . et al ., 1999; Kronewitter, S .R ., 2010) . N-glycans play a major role in regulating biochemical pathways and in the communication at the cellular level between cells, tissue and organs (Shriver at al ., 2004) . As such, there is a great deal of interest in the study of glycosylation and N-glycans as biomarkers for a variety of diseases (Lebrilla, C .B ., 2009; deLeoz, M .L .A . et al ., 2008; Turnbull, J .E . et al ., 2007) . This application note describes a high throughput sample preparation procedure for global glycan massprofiling . The Gilson GX-271 ASPEC™ System was used for this application .

Identification and Quantification of Illegal Drugs and Benzodiazepines in Human Plasma by LC/MS-MS Analysis after Solid Phase Extraction (SPE)


The identification and quantification of drugs of abuse in blood (plasma, serum, whole blood) has become very common in the forensic medicine laboratory . A variety of methods for the extraction and analysis of these drugs have been described in the literature (Sadeg and Dumontet, 2001; Moeller and Kraemer, 2002), with particular focus on the use of GC/MS or LC/MS for analysis . Liquid/liquid extraction (LLE) has been traditionally used at a basic pH (pH = 9) for the extraction of amphetamines, cocaine and its metabolites and opiates from blood . This is sometimes followed by an additional clean-up step . This study describes an automated SPE protocol using the Gilson GX-271 ASPEC System .

Automated Solid Phase Extraction of Allantoin from Cosmetics and Topical Pharmaceuticals Prior to Analysis by HPLC


Allantoin is a heterocyclic organic compound derived from purine . It is also referred to as Glyoxylic Acid Diureide or 5-Ureidhyantoin . Allantoin is a metabolic end product of purine degradation in mammals (with the exception of humans and higher apes), as well as a metabolic intermediate in plants and some bacteria (Young et al ., 1944; Fujiwara et al ., 1995) . Allantoin can be found in many plant species . Comfrey (Symphytum officinale) has particularly high levels of allantoin . Allantoin has a long history of use in a variety of topical pharmaceuticals and cosmetics for skin care . It has keratolytic, moisturizing, soothing and anti-irritant properties . Allantoin promotes the renewal of epidermal cells and accelerates wound healing (AKEMA, 2008) . Allantoin is used in pharmaceuticals and dermatologic products in the treatment of ulcers, slow-healing wounds, burns, psoriasis and dry skin . There is a great deal of interest from pharmaceutical and cosmetic manufacturers in determining the amount of allantoin in a variety of products . This application note describes an automated SPE method using the Gilson GX-271 ASPEC™ System for the extraction of allantoin from a cosmetic product .

The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary Supplements

Using the Gilson 215 Analytical to Semi-preparative HPLC System

Nutraceuticals or “phytoceuticals” can be defined as natural products that are used to supplement the diet by increasing the total dietary intake of important nutrients . This definition includes nutritional supplements such as vitamins, minerals, herbal extracts, antioxidants, amino acids and proteins . Ephedra is a naturally-occurring substance derived from the Chinese herbal Ma Huang . Its principle active ingredient is ephedrine, which, when chemically synthesized, is regulated as a drug . The FDA’s concerns about dietary supplements containing ephedra arise in part from ephedra’s mechanism of action in the body . Ephedra is an adrenaline-like stimulant that can have potentially dangerous effects on the nervous system and

An Automated Method for Dispensing Fruit Fly (Drosophila malonogaster) Food into Culture Vials and Bottles

Using the Gilson 215 Liquid Handler

The vinegar fly or fruit fly, Drosophila melanogaster, has been utilized in medical and biological research laboratories for over 100 years . It was one of the first organisms used for genetic research and is the genetically best-known of all eukaryotic organisms . Research in the past twenty years has demonstrated that humans and fruit flies are very similar at the molecular level . About 75% of known human disease genes have a recognizable match in the genetic code of Drosophila melanogaster (Reiter, et al ., 2001) and 50% of fly protein sequences have mammalian analogs . Thus, D . melanogaster is an excellent animal model for studying a variety of human diseases . This application note describes the use of an automated instrument for filling large numbers of culture vials with fly food or fly culture media using the Gilson 215 Liquid Handler .

heart . Ephedrine has a chemical structure similar to amphetamines, differing only in that ephedrine is less potent . The purpose of this application is to present the advantages of using an automated solid phase extraction method coupled with HPLC to determine the various levels of ephedra and ephedra-like compounds in dietary supplements versus manual solidphase extraction . The Gilson 215 Analytical to Semi-preparative HPLC System was used for this application .

Automated Fractionation System that Increases Recovery and Optimize Precision of Solid Phase Extraction Methodology


Solid Phase Extraction has become a technique of choice for sample cleanup and trace Enrichment . Some of the benefits of SPE are: Versatility, Selectivity, Speed and Low Solvent usage . There are other methods for sample cleanup, such as Liquid-Liquid Extraction, Protein Precipitation, Turbulent Flow and Dialysis . Most automated methods find their roots in a manual method, but most manual methods don’t optimize the extraction mechanism due to the so called “time” believed to be involved in method development . Automation in its most general term removes the repetitive/monotonous nature associated with method development and the drain on human capital, while increasing recovery and optimizing precision . The Gilson GX-271 ASPEC System was used for this application .

GX-271 ASPEC™GX-271 ASPEC™

215 Liquid Handler

GX-274 ASPEC™

215 Analytical to Semi-prep HPLC System


Applications


Tip Design Description Features

Flat The tip is cut perpendicular to the length of the probe .

Aspirates the last drop of liquid

Does not pierce septum

No injection

Constricted The tip of the probe has a smaller outer diameter than the rest of the shaft . Constricted-tip probes accommodate unique tip designs optimizing every application .

Compatible with injection ports

Beveled The tip is cut at an angle, leaving a bevel . Sharp tip enables septum-piercing


Spring Loaded Low volume, touch-off dispensing . Ideal for MALDI spotting applications

Spring loaded probe removes any potential of damaging spotting surface

Vented Two styles:

1 . A shallow groove cut in the probe shaft, above the tip (shown) .

2 . Two probes are assembled concentrically, creating two separate channels, the inner channel for liquid and the outer channel for gas .

Vented probes may have different tip designs .

Sharp-tip enables septum-piercing

Vent allows pressures inside and outside a sealed vessel to equilibrate as liquid is dispensed or aspirated

Side-entry The liquid flow path opens on the side of the probe, not at the tip .

Recommended for thick septa

Prevents coring of septa


Not recommended with limited volume

Deflected Based on the beveled tip design, the probe tip is curved and very sharp .

Sharp-tip enables septum-piercing

Tip pushes septa out of the way as it pierces to prevent coring

Conical Designed for PEEK injection ports . Capable of piercing thin septa

Aspirates the last drop of liquid

Gilson offers many different types of probes, probe tips and coatings to meet any and all application needs providing solutions for all liquid handling requirements .

Probes

Periodic Table of the Elements

1H

1.008

2He

4.003

87Fr

(223)

103Lr

(260)

102No

(259)

101Md

(258)

100Fm

(257)

99Es

(252)

98Cf

(251)

97Bk

(247)

96Cm

(247)

95Am

(243)

71Lu

175.0

70Yb

173.0

69Tm

168.9

68Er

167.3

67Ho

164.9

66Dy

162.5

65Tb

158.9

64Gd

157.3

63Eu

152.0

94Pu

(244)

93Np

237.0

62Sm

150.4

61Pm

(145)

92U

238.0

91Pa

231.0

60Nd

144.2

59Pr

140.9

90Th

232.0

58Ce

140.1

**Actinides

*Lanthanides

Undiscovered Synthetic From Decay Primordial

*

**105Ha

(262)

104Rf

(261)

89Ac

227.0

88Ra

226.0

109Une

(276) (281) (280) (285) (284) (289) (288) (291) (294)Not Yet Observed(277)

107Uns

108Hs

110Ds

111Rg

112Uub

113Uut

114Uuq

115Uup

116Uuh

117Uus

118Uuo

(262)

106Unh

(263)

55Cs

132.9

86Rn

(222)

85At

(210)

84Po

(209)

83Bi

209.0

82Pb

207.2

81Tl

204.4

80Hg

200.6

79Au

197.0

78Pt

195.1

73Ta

180.9

72Hf

178.5

57La

138.9

56Ba

137.3

77Ir

192.2

76Os

190.2

75Re

186.2

74W

183.9

37Rb

85.47

54Xe

131.3

53I

126.9

52Te

127.6

51Sb

121.8

50Sn

118.7

49In

114.8

48Cd

112.4

47Ag

107.9

46Pd

106.4

41Nb

92.91

40Zr

91.22

39Y

88.91

38Sr

87.62

45Rh

102.9

44Ru

101.1

43Tc

(98)

42Mo

95.94

19K

39.10

36Kr

83.80

35Br

79.90

34Se

78.96

33As

74.92

32Ge

72.59

31Ga

69.72

30Zn

65.38

29Cu

63.55

28Ni

58.70

23V

50.94

22Ti

47.90

21Sc

44.96

20Ca

40.08

27Co

58.93

26Fe

55.85

25Mn

54.94

24Cr

52.00

11Na

23.00

18Ar

39.95

17Cl

35.45

16S

32.06

15P

30.97

14Si

28.09

13Al

26.98

12Mg

24.31

3Li

6.941

10Ne

20.18

9F

19.00

8O

16.00

7N

14.01

6C

12.01

5B

10.81

4Be

9.012

1A 8A

2B1B5B4B3B 8B7B6B

7A6A5A4A3A2A

Capillary Flow Cell

Micro-bore Flow Cell

Analytical Flow Cell

Semi-preparative Flow Cell

Preparative Flow Cell

Product # 104446 104442 104445 104441 104443

Internal Volume

35 nL 1 .5 µL 12 µL 0 .7 µL 0 .16 µL

Path length 8 mm 2 mm 5 mm 0 .2 mm 0 .05 mm

Column Size 180 – 300 µm 1 – 2 mm 4 mm

10 mm

20 mm > 40 mm

Flow Rate 1 – 10 µL/min 25 – 200 µL/min 1 – 15 mL/min 15 – 50 mL/min 30 – 200 mL/min

Purify µg - 5 mg Purify 5 – 300 mg Purify 300 mg - 15 g

Selecting the correct flow cell will optimize sensitivity and peak shape . The following chart pairs flow cells with the appropriate column, flow rate and sample concentration to help choose the correct flow cell for the application .

Flow Cell Selection Chart for Optimizing Sensitivity on 15X Series Detectors

References


References


Maximum UV Absorbance for Commonly Used Solvents Operating at a wavelength near or below a mobile phase solvent’s maximum UV absorbency increases baseline noise due to the UV response of the solvent . Refer to the tables below to optimize chromatographic conditions .

Table 2

Mobile Phase Conditioners & Buffers Maximum UV Absorbance (nm)

Acetic Acid, 1% 230

Ammonium Acetate, 10 mM 205

Ammonium Bicarbonate, 10 mM 190

Diammonium Phosphate, 50 mM 205

EDTA, Disodium, 1 mM 190

HEPES, 10 mM, pH 7 .6 225

Hydrochloric acid, 0 .1% 190

MES, 10 mM, pH 6 .0 215

Potassium Phosphate Monobasic, 10 mM Dibasic, 10 mM

190 190

Sodium Acetate, 10 mM 205

Sodium Chloride, 1M 207

Sodium Citrate, 10 mM 225

Sodium Dodecyl Sulfate 190

Sodium Formate 200

Triethylamine, 1% 235

Trifluoracetic Acid, 0 .1% 190

TRIS HCl, 20 mM pH 7 .0 pH 8 .0

202 212

pH of Common Bases

Bases (pH > 7) Molarity pH

Ammonia 1 N 11 .6

Ammonia 0 .1 N 11 .1

Ammonia 0 .01 N 10 .6

Barbital Sodium 0 .1 N 9 .4

Borax 0 .01 N 9 .2

Calcium Carbonate Saturated 9 .4

Calcium Hydroxide Saturated 12 .4

Ferrous Hydroxide Saturated 9 .5

Lime Saturated 12 .4

Magnesia Saturated 10 .5

Potassium Acetate 0 .1 N 9 .7

Potassium Bicarbonate 0 .1 N 8 .2

Potassium Carbonate 0 .1 11 .5

Potassium Cyanide 0 .1 N 11

Potassium Hydroxide 1 N 14

Potassium Hydroxide 0 .1 N 13

Potassium Hydroxide 0 .01 N 12

Sodium Acetate 0 .1 N 8 .9

Sodium Benzoate 0 .1 N 8

Sodium Bicarbonate 0 .1 N 8 .4

Sodium Carbonate 0 .1 N 11 .6

Sodium Hydroxide 1 N 14

Sodium Hydroxide 0 .1 N 13

Sodium Hydroxide 0 .01 N 12

Sodium Metasilicate 0 .1 N 12 .6

Sodium Sesquicarbonate 0 .1 N 10 .1

Trisodium Phosphate 0 .1 N 12

Internal Diameter (mm) Typical Injection Volume (µL) Load Capacity (mg) Typical Flow Rates (mL/min)

4 .6 15 1 0 .5 – 2

10 100 4 .7 4 – 15

21 .2 400 19 .5 10 – 50

30 1,000 42 .5 40 – 100

50 2,000 118 100 – 300

100 10,000 473 400 –1,000

pH of Acids & Bases

Column Sizes & Capacities

Table 1

Solvent Maximum UV Absorbance (nm)

1-Nitropropane 380

2-Butoxyethanol 220

Acetone 330

Acetonitrile 190

Amyl Alcohol 210

Amyl Chloride 225

Benzene 280

Butanol-1 215

Butanol-2 260

n-Butyl Chloride 220

Carbon Disulfide 380

Carbon Tetrachloride 265

Chloroform 245

Clorobenzene 287

Cyclohexane 200

Cyclopentane 200

Diethyl Amine 275

Dimethyl Sulfoxide 268

Dioxane 215

Ethanol 210

Ethyl Acetate 256

Ethyl Ether 220

Ethyl Sulfide 290

Ethylene Dichloride 230

Ethylene Glycol 210

Heptane 200

Hexane 195

Isooctane 215

Isopropanol 205

Isopropyl Chloride 225

Isopropyl Ether 220

Methanol 205

Methyl Acetate 260

Methyl Ethyl Ketone 330

Methyl Isobutyl Ketone 334

Methylene Chloride 233

n-Pentane 190

n-Propanol 210

n-Propyl Chloride 225

Nitromethane 380

Petroleum Ether 210

Pyridine 330

Tetrahydrofuran 230

Toluene 285

Xylene 290

pH of Common Acids

Acids (pH < 7) Molarity pH

Acetic 1 N 2 .4

Acetic 0 .1 N 2 .9

Acetic 0 .01 N 3 .4

Alum 0 .1 N 3 .2

Arsenious Saturated 5

Benzoic 0 .1 N 3

Boric 0 .1 N 5 .3

Carbonic Saturated 3 .8

Citric 0 .1 N 2 .1

Formic 0 .1 N 2 .3

Hydrochloric 1 N 0 .1

Hydrochloric 0 .1 N 1

Hydrochloric 0 .01 N 2

Hydrocyanic 0 .1 N 5 .1

Hydrogen Sulfide 0 .1 N 4 .1

Lactic 0 .1 N 2 .4

Malic 0 .1 N 2 .2

Nitric 0 .1 N 1

Orthophosphoric 0 .1 N 1 .5

Oxalic 0 .1 N 1 .3

Succinic 0 .1 N 2 .7

Salicylic Saturated 2 .4

Sulfuric 1 N 0 .3

Sulfuric 0 .1 N 1 .2

Sulfuric 0 .01 N 2 .1

Sulfurous 0 .1 N 1 .5

Tartaric 0 .1 N 2

Trichloracetic 0 .1 N 1 .2


References


Separation Scale-up Equations

Table 2: Rheodyne 7010 – 156 Factory Installed Large-bore Rotor Seal

Flow Rate (mL/min)

Valve Position and Pressure

Standard 7010 Bore Size 7010 with Large-bore Rotor

Load Position Pressure

(psi)

Inject Position Pressure (psi)

Gilson Installed Load Position Pressure (psi)

Gilson Installed Inject Position Pressure (psi)

25 0 14 .5 0 0 – 14 .5

50 0 43 .5 0 43 .5

75 0 87 0 116

100 14 .5 145 14 .5 203 .1

150 43 .5 290 72 .5 435 .1

200 87 435 .1 145 696 .2

Optimizing Preparative HPLC There are numerous parameters and techniques to optimize preparative HPLC . These reference tables provide important physical parameters of the tubing and injection valve structure; optimization will improve chromatography and percent recovery of collected fractions .

Table 1: 33X Series pump tubing (PEEK)*

Flow Rate (mL/min)

Tubing ID (in)

0.02 Pressure (psi) 0.03 Pressure (psi) 0.04 Pressure (psi)

25 0 0 0

50 43 .5 0 0

75 101 .5 0 0

100 203 .1 14 .5 0

125 333 .6 43 .5 0

150 464 .1 87 14 .5

175 623 .7 101 .5 14 .5

200 797 .7 145 29*30” lengths of tubing used at corresponding flow rates for tubing pressure. Tubing was connected directly to the pump. All pre-filters were removed.

At high flow rates tubing can cause high back pressure . Ensuring that the appropriate tubing ID/flow rate combination is used will optimize the pumping system .

Product #: 25051043 (7010 valve with large-bore rotor seal)

0 .018" make-before-break stator orifice .

1 mL stainless steel 0 .030" ID loop .

All other plumbing used is stainless steel 0 .040" ID tubing .

Solvent Miscibility & Polarity

Solvent Miscibility Table Solvent Polarity Index

Refraction Index @20°C

UV(nm) Cut-off @1AU

Boiling Point (°C)

Viscosity (cPoise)

Solubility in water (%w/w)

Acetic Acid 6 .2 1 .372 230 118 1 .26 100Acetone 5 .1 1 .359 330 56 .032 100Acetonitrile 5 .8 1 .344 190 82 0 .37 100Benzene 2 .7 1 .501 280 80 0 .65 0 .18n-Butanol 4 .0 1 .394 254 125 0 .73 0 .43Butyl Acetate 3 .9 1 .399 215 118 2 .98 7 .81Carbon Tetrachloride 1 .6 1 .466 263 77 0 .97 0 .08Chloroform 4 .1 1 .446 245 61 0 .57 0 .815Cyclohexane 0 .2 1 .426 200 81 1 .00 0 .011,2-Dichloroethane1 3 .5 1 .444 225 84 0 .79 0 .81Dichloromethane2 3 .1 1 .424 235 41 0 .44 1 .6Dimethylformamide 6 .4 1 .431 268 155 0 .92 100Dimethyl Sulfoxide3 7 .2 1 .478 268 189 2 .00 100Dioxane 4 .8 1 .422 215 101 1 .54 100Ethanol 5 .2 1 .360 210 78 1 .20 100Ethyl Acetate 4 .4 1 .372 260 77 0 .45 8 .7Di-Ethyl Ether 2 .8 1 .353 220 35 0 .32 6 .89Heptane 0 .0 1 .387 200 98 0 .39 0 .0003Hexane 0 .0 1 .375 200 69 0 .33 0 .001Methanol 5 .1 1 .329 205 65 0 .60 100Methyl-t-Butyl Ether4 2 .5 1 .369 210 55 0 .27 4 .8Methyl Ethyl Ketone5 4 .7 1 .379 329 80 0 .45 24Pentane 0 .0 1 .358 200 36 0 .23 .004n-Propanol 4 .0 1 .384 210 97 2 .27 100Iso-Propanol6 3 .9 1 .377 210 82 2 .30 100Di-Iso-Propyl Ether 2 .2 1 .368 220 68 0 .37Tetrahydrofuran 4 .0 1 .407 215 65 0 .55 100Toluene 2 .4 1 .496 285 111 0 .59 0 .051Tichloroethylene 1 .0 1 .477 273 87 0 .57 0 .11Water 9 .0 1 .333 200 100 1 .00 100Xylene 2 .5 1 .500 290 139 0 .61 0 .018

Xyle

neW

ater

Tich

loro

ethy

lene

Tolu

ene

Tetr

ahyd

rofu

ran

Di-I

so-P

ropy

l Eth

erIs

o-Pr

opan

ol6

n-Pr

opan

olPe

ntan

eM

ethy

l Eth

yl K

eton

e5

Met

hyl-t

-But

yl E

ther

4

Met

hano

lH

exan

eH

epta

neD

i-Eth

yl E

ther

Ethy

l Ace

tate

Etha

nol

Dio

xane

Dim

ethy

l Sul

foxi

de3

Dim

ethy

lform

amid

eD

ichl

orom

etha

ne2

1,2-

Dic

hlor

oeth

ane1

Cycl

ohex

ane

Chlo

rofo

rmCa

rbon

Tetr

achl

orid

eBu

tyl A

ceta

ten-

Buta

nol

Benz

ene

Acet

onitr

ileAc

eton

eAc

etic

Aci

d

Immiscible

Miscible

Immiscible means that in some proportions two phases will be produced.

Synonym Table1 Ethylene Chloride2 Methylene Chloride3 Methyl Sulfoxide4 tert-Butyl Methyl Ether5 2-Butanone6 2-Propanol

Solvent Polarity Chart

Relative Polarity

Compound Formula

Group Representative Solvent Compounds

Non-polar R - H Alkanes Petroleum Ethers, Ligroin, Hexanes

Ar - H Aromatics Toluene, Benzene

R - O - R Ethers Diethyl Ether

R - X Alkyl Halides Tetrachloromethane, Chloroform

R - COOR Esters Ethyl Acetate

R - CO - R Aldehydes and Ketones

Acetone, Methyl Ethyl, Ketone

R - NH 2 Amines Pyridine, Triethylamine

R - OH Alcohols Methanol, Ethanol, Isopropanol, Butanol

R - COHN 2 Amides Dimethylformamide

R - COOH Carboxylic Acids Ethanoic Acid

Polar H - OH Water Water

Incr

easi

ng P

olar

ity

Scale-up Factor (diameter preparative)2 scale-up factor = (diameter analytical)2

Flow Rate scale-up factorflow rate preparative = analytical flow rate

Gradient Duration (GD) length preparative (diameter preparative)2 flow rate analyticalGD preparative = GD analytical x x x length analytical (diameter analytical)2 flow rate preparative


References


Common Conversion Tables

Temperature Conversion Formulas

˚C = ˚F – 32 ÷ 1 .8 ˚C = Degrees Celsius

˚F = Degrees Fahrenheit

˚K = Degrees Kelvin

˚R = Degrees Rankine

˚C = ˚K – 273 .15

˚F = 1 .8 x ˚C + 32

˚F = ˚R – 459 .67

˚K = ˚C + 273 .15

˚R = ˚F + 459 .67

Pressure Conversion

kPa psi bar MPa

690 100 6 .9 0 .7

3450 500 34 .5 3 .4

6890 1000 68 .9 6 .9

10340 1500 103 .4 10 .3

13790 2000 137 .9 13 .8

17240 2500 172 .4 17 .2

20680 3000 206 .8 20 .7

24130 3500 241 .3 24 .1

27580 4000 275 .8 27 .6

31030 4500 310 .3 31

34470 5000 344 .7 34 .5

37920 5500 379 .2 37 .9

41370 6000 413 .7 41 .4

44820 6500 448 .2 44 .8

48260 7000 482 .6 48 .3

51710 7500 517 .1 51 .7

55160 8000 551 .6 55 .2

58610 8500 586 .1 58 .6

62050 9000 620 .5 62 .1

65500 9500 655 65 .5

68950 10000 689 .5 68 .9

Temperature Conversion

Celsius (°C) Fahrenheit (°F)

-100 -148

-50 -58

-40 -40

-30 -22

-20 -4

-10 14

0 32

5 41

10 50

15 59

20 68

25 77

30 86

35 95

40 104

45 113

50 122

55 131

60 140

65 149

70 158

75 167

80 176

85 185

90 194

95 203

100 212

105 221

110 230

115 239

120 248

125 257

130 266

135 275

140 284

145 293

150 302

155 311

160 320

165 329

170 338

175 347

180 356

185 365

190 374

195 383

200 392

Fractional inch-to-decimal- to-millimeter Conversion

Fraction (") Decimal Millimeter

1/32 .0313 .7938

1/16 .0625 1 .5875

3/32 .0938 2 .3813

1/8 .125 3 .175

5/32 .1563 3 .9688

3/16 .1875 4 .7625

7/32 .2188 5 .5563

1/4 .25 6 .35

9/32 .2813 7 .1438

5/16 .3125 7 .9375

11/32 .3438 8 .7313

3/8 .375 9 .525

13/32 .4063 10 .3188

7/16 .4375 11 .1125

15/32 .4688 11 .9063

1/2 .5 12 .7

17/32 .5313 13 .4938

9/16 .5625 14 .2875

19/32 .5938 15 .0813

5/8 .625 15 .875

21/32 .6563 16 .6688

11/16 .6875 17 .4625

23/32 .7188 18 .2563

3/4 .75 19 .05

25/32 .7813 19 .8438

13/16 .8125 20 .6375

27/32 .8438 21 .4313

7/8 .875 22 .225

29/32 .9063 23 .0188

15/16 .9375 23 .8125

31/32 .9688 24 .6063

1 1 25 .4


Gilson Quality Policy

It is Gilson’s policy to promote a total commitment to quality by each individual throughout the Gilson organization worldwide . It is our goal that this commitment to quality will result in complete customer satisfaction with both Gilson products and services .

ISO 9001 Certification

Gilson is committed to quality, and it shows in everything Gilson does – from design to manufacturing, technical support and training . Due to this commitment to quality and customer satisfaction, Gilson has consistently been awarded with ISO 9001 certification . Gilson, Inc . and Gilson S .A .S . are both ISO 9001 certified .

Customer Education Programs

Gilson offers industry-leading education for our global customers through our web-based courses, complimentary web seminars, local workshops, and classroom training courses . From travel-free education to our hands-on traditional training options, Gilson provides an extensive program to meet the needs of those just getting started and those looking to advance their skills .

Visit www.gilson.com or contact training@gilson .com for our full Pipettes & Tips or SPE & Purification programs .

High quality of conception and calibration of our products

Our pipettes are designed, manufactured and calibrated in compliance with the specifications and methods of the ISO 8655 standard .

Guarantee of our pipettes’ safety for In Vitro Diagnostics

All our pipettes comply with the IVD Directive (98/79/ EC) for In Vitro Diagnostics Medical Devices .

Represented in Over 80 Countries WorldwideTo find a Gilson representative nearest to you, click “Where to Order” at www.gilson.com

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Automated Liquid HandlingProduct Guide

LT303041-11 © 2011 Gilson Inc. All rights reserved. All trademarks are the property of Gilson Inc. and its subsidiaries.

Gilson, Inc. World Headquarters | P.O. Box 620027 | Middleton, WI 53562-0027 | Tel: 800-445-7661 | Fax: 608-831-7661


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