Catalog 2016 - westburg.eu · ThawSTAR® CFT2 Instrument (green traces), a 37°C bead bath (Lab...

Catalog 2016

Intuitivestandardization.Prep.Freeze.Transport.Store.Thaw.

Note: As of January 1, 2016, BioCision entered into a global distribution partnership with Corning, Incorporated. CoolCell®, CoolBox™, CoolRack®, CoolSink®, ThermalTray™, CryoCeps™ and TruCool® ice pans and buckets are now available as Corning branded items through Fisher Scientific, Fisher Healthcare, Thermo Fisher Scientific and VWR International. All other BioCision brands (ThawSTAR®, CryoPod™, BioT™, SafeStream™ and TruCool® accessories) are not affected by this change and are still available as BioCision branded products.

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CoolCell® Controlled-rate, alcohol-free cell freezing containersPages 15 - 18

ThermalTray™ Thermo-conductive platformsPage 28

TruCool® AccessoriesPage 29 - 30

ThawSTAR® Automated cell thawing systemPages 3 - 6

CryoPod™

Portable liquid nitrogen carrier Pages 7 - 8

CoolBox™ Ice-free cooling and freezing systemsPages 19 - 22

CoolRack® Thermo-conductive tube modulesPages 23 - 26

CoolSink® Thermo-conductive plate modules Page 27

BioT™ Temperature stability systemsPages 9 - 14

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ThawSTAR® Systems Automated Cell Thawing

ThawSTAR® Cell Transport and Automated Thawing System is a first-of-its-kind technology platform that combines ultra-low temperature vial transport and automated cell thawing in a convenient and compact format. The system replaces manual methods of cell thawing - such as swirling frozen vials in communal water baths and warming vials between hands - that are not standardized and potentially jeopardize the integrity and safety of the sample, and enables thawing in a cell culture hood.

How It Works

ThawSTAR® Automated Cell Thawing Instrument features intuitive operation, making integration and standardization easy. Simply insert a frozen cryogenic vial and retrieve it when the vial is gently raised at the end of the thaw cycle. The small footprint and audio/visual feedback enable use in a cell culture hood with the rest of the workflow.

Reproducibility

Six vials were thawed in a 37°C water bath. The average thaw time in the water bath was 151 s, with a 99% confidence interval of 139-164 s (range shown as dotted lines). Left: The avg. time for the same ThawSTAR® instrument to thaw > 5 vials was measured each day for 5 days. Right: The avg. thaw time for 3 different ThawSTAR instruments was measured using > 5 vials per instrument on the same day. No significant differences were identified for either scenario at p<0.05 (2-way ANOVA with post hoc Sidak test).

3 ThawSTAR instruments tested on same day

Same ThawSTAR instrument tested on 5 days

PROBLEM: Water Baths and Other Manual Thawing Methods• User technique dependent• Variable end points• Contamination risk• Not standardized

SOLUTION: ThawSTAR® Cell Transport and Automated Thawing System • De-risk cell handling prior to thaw• Highly reproducible thaw outcome• Ideal for GMP and point-of-care use• Remove subjectivity and variability

Instrument, Test Date Instrument, Test Date

99% CI of water bath thaw time

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PBMC. Cell viability (left panel) and recovery (right panel) of PBMC were measured using trypan blue exclusion on a hemocytometer. Recovery is the number of viable cells post-thaw as a percentage of the pr xe-freeze viable cells. No statistical difference in viability (2-way ANOVA with post hoc Sidak test) or recovery (unpaired two-tailed t-test) at p<0.05 were found.

- Data generated by Dr. Mars Stone at Blood Systems Research Institute

Cell Viability

Viability Recovery

PBMC Cell Recovery and Consistency

Mean recovery is compared for paired PBMCs isolated immediately after phlebotomy using traditional Ficoll and SepMate methods (left) (n = 6). Mean recovery post cryopreservation is compared for paired PBMCs isolated immediately after phlebotomy for cells thawed in a water bath and using the ThawSTAR® CFT2 Instrument (right) (n = 3).

- Data generated by Blood Systems Research Institute

Cryopreserved

Water Bath ThawSTAR®

Performance Data vs. Water Bath

PBMC Post-Thaw Viability and Consistency

Mean viability is compared for paired peripheral blood mononuclear cells (PBMCs) isolated immediately after phlebotomy using traditional Ficoll and SepMate methods (left). Mean viability is compared post cryopreservation of paired PBMCs isolated immediately after phlebotomy for cells thawed in a water bath and using ThawSTAR® CFT2 Instrument (right). With fresh isolates, duplicate samples were processed by two different technicians to compare intertechnician variability for both PBMC isolation methods (results not shown) (n = 6). For cryopreserved samples, one vial from each of three donors was thawed for each condition being analyzed (n = 3).

- Data generated by Blood Systems Research Institute

Cryopreserved

Water Bath ThawSTAR®

Ex-Vivo B Cells Post-Thaw Viability and Consistency Compared to Water Bath

Ex-vivo stimulated B cells: A total of 6 vials, 2 each from 3 donors, were frozen in a -80°C freezer using a CoolCell® module and then stored in liquid nitrogen for two weeks. One vial from each donor was removed from LN2, placed into a ThawSTAR® CFT Transporter and thawed in a ThawSTAR® CFT2 automated cell thawing instrument. The other vial from each donor was removed from LN2 and hand-carried to a water bath. The vials transported and thawed in the ThawSTAR transport and thawing system performed better in terms of cell viability and recovery. - Data generated by UCSF Diabetes Center and Transplant Surgery

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ThawSTAR® CFT2 Transporter is comprised of an outer insulative foam lid and base, and an internal round CoolRack® CFT2 sample module that accommodates up to five 1.8 or 2.0 mL cryogenic vials. Prior to using the ThawSTAR CFT2 Transporter, a thin layer of dry ice is added to the bottom of the base. The round CoolRack CFT2 module is placed over the dry ice and allowed to equilibrate to dry-ice temperature (approximately 20 minutes). Frozen vial is then extracted from storage and quickly placed into the module. The lid is placed securely on top of the base and all samples are maintained at dry ice temperature until transported to the thawing location.

How It Works

Thermal Profile

1.8 mL cryogenic vials filled with 1.0 mL of cryopreservation medium (10% DMSO/20% FBS/70% DMEM) were fitted with an interior wall thermocouple (< 0.5 mm from internal wall surface) and a central axis thermocouple, frozen at -80°C, and maintained at -75°C in a CoolRack® on dry ice. Three vials were thawed in a 37°C water bath (left panel) or in the ThawSTAR® CFT2 Instrument (right panel). For the water bath thaw, the vials were removed from the bath when a pea-sized ice chunk remained (arrow) and then gently tapped to melt the chunk. Similarly, ThawSTAR instrument ejected the vial at the point where a pea-sized ice chunk remained (arrow) and after mixing the final vial temperature is ~5-10°C. Note that the sharp rise in temperature seen with the central thermocouple is indicative of the ice chunk breaking away from the thermocouple. The temperature profiles recorded were practically identical for both the water bath and ThawSTAR thaw.

Compared to Water Bath

ThawSTAR® CFT Transporter

Performance Data

ThawSTAR® CFT2 Transporter holds cryogenic vials at near dry ice temperatures prior to thawing in the ThawSTAR® CFT2 Instrument. Dry ice is placed in the foam holder below the metal CoolRack® module. To verify the holding temperature, a cryogenic vial filled with cryopreservative solution with a centrally-located thermocouple was frozen in LN2 then transferred to the pre-equilibrated CFT Transporter. The temperature profile shows that the vial equilibrates quickly (< 20 min) to the holding temperature and remains stable for hours.

1.8 mL cryogenic vials filled with 1.0 mL of cryopreservation medium (10% DMSO/20% FBS/70% DMEM) were fitted with a wall thermocouple (< 1 mm from internal wall surface) and a central axis thermocouple, frozen at -80°C, and maintained at -75°C in a CoolRack® on dry ice. Vials were thawed in either a ThawSTAR® CFT2 Instrument (green traces), a 37°C bead bath (Lab Armor beads; red traces), or an aluminum heat block equilibrated to 37°C (blue traces). The ThawSTAR Instrument thaw time is 2-3X faster than other dry thawing methods tested here.

Compared to Dry Bead Bath or Heat Block

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Ordering Information

The ThawSTAR® System is for laboratory research use only. Any intended use for diagnostic purposes, direct transfusion, or in the production of therapeutic product(s) or vaccines(s) may require advance regulatory clearance which is the sole responsibility of the user, as this is not a medical device that has undergone medical device registration, clearance, or approval by the U.S. Food and Drug Administration (FDA), European Union, Health Canada, or the Australian Therapeutic Goods Administration. Research Only Device: Limited by Federal Law (United States) to Research Use Only.

Item No. Description

BCS-600 ThawSTAR® CFT2 Cell Transport and Thawing System, complete

BCS-601 ThawSTAR® CFT2 Automated Cell Thawing Instrument

BCS-602 ThawSTAR® CFT2 Transporter for up to five 1.8 or 2.0 mL cryogenic vials

BCS-603 ThawSTAR® CFT2 Confirmation Vials

BCS-614 IOP Qualification Packet for ThawSTAR® Automated Cell Thawing System, Model CFT2

BCS-618 ThawSTAR® CFT2 Complete System and IOPQ Bundle

BCS-630 ThawSTAR® CFT1.5 Cell Transport and Thawing System, complete

BCS-631 ThawSTAR® CFT1.5 Automated Cell Thawing Instrument

BCS-632 ThawSTAR® CFT1.5 Transporter for up to five 1.5 mL cryogenic vials

BCS-633 ThawSTAR® CFT1.5 Confirmation Vials

BCS-620 ThawSTAR® AT6 Cell Transport and Thawing System, complete

BCS-621 ThawSTAR® AT6 Automated Cell Thawing Instrument

BCS-622 ThawSTAR® AT6 Transporter for up to six 6.0 mL AT Closed Vials®

BCS-610 ThawSTAR® Rapid Check and Preventative Maintenance (North America)

BCS-611 ThawSTAR® Extended Limited Warranty (North America)

BCS-612 ThawSTAR® Rapid Check and Preventative Maintenance (Intl)

BCS-613 ThawSTAR® Extended Limited Warranty (Intl)

ThawSTAR® CFT2 Instrument

Dimensions (Diameter x Height) 11.0 cm x 14.5 cm (5.7 in x 4.3 in)

Capacity 1.8 - 2.0 mL cryogenic vial

Fill volume 0.8 - 1.5 mL

Vial brand Compatible with major vial manufacturers

Thawing time Less than 3 minutes

ThawSTAR® CFT2 Transporter

Dimensions (Diameter x Height) 11.4 x 15.0 cm (4.5 x 6.0 in)

Dry ice used ~150 g

Holding temperature < -70°C

ThawSTAR® CFT1.5 Instrument


Capacity 1.5 mL cryogenic vial


Vial brand Nalgene®

Thawing time Less than 3 minutes

ThawSTAR® CFT1.5 Transporter

Dimensions (Diameter x Height) 11.4 x 15.0 cm (4.5 x 6.0 in)

Dry ice used ~150 g


ThawSTAR® AT6 Instrument


Capacity 6.0 mL AT-Closed Vial®


Vial brand Aseptic Technologies®

Thawing time 6 - 10 minutes

ThawSTAR® AT6 Transporter

Dimensions (L x W x H) 20.0 x 16.0 x 16.0 cm (7.8 x 6.3 x 6.3 in)

Dry ice used ~250 g


Specifications

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CryoPod™ Carrier provides a safe, reliable and portable < -150°C cryogenic environment for the handling and transport of biological specimens for over 4 hours. The instrument displays and logs temperature, date and time, and features audible and visual alarms, and integrates into an optional automated filling station for hands-free replenishing of the LN2 charge in less than 15 minutes.

Winner of two innovation awards!• New Product of the Year Award at ISBER 2015 • New Innovative Product or Technology Award at ESBB 2015

Ensures operator safety• Allows safe and quick transportation of cryogenic samples• Hands-free auto-fill option

Maintains sample cold chain integrity• Temperature display with audible and visual alarms• Temperature logging and retrieval

Delivers reliable performance• Over 4 hours <-150°C• No direct sample contact with LN2

Portable• Compact footprint; only ~9 lbs• Built-in handle and bottom finger grips

CryoPod™ CarrierPortable Liquid Nitrogen (LN2)-Based Cryogenic Transport

Over 4 Hour Hold Time Stable Temperature While Transporting

(1) Cryobox sitting in cryostorage container with 0.5 inches of LN2 prior to insertion into CryoPod™ Carrier. Samples in cryobox <-170°C. (2) Cryobox inserted into CryoPod Carrier. (3) CryoPod Carrier transported by foot around local vicinity for 1.5 hours. Ambient temperature of 18-21°C (65-70°F). (4) Cryobox removed from CryoPod Carrier and inserted into LN2 cryostorage container.

The entire 2.5 inch working depth of the CryoPod™ Carrier remains well below -150°C for over 4 hours with one charge of LN2 and with the lid closed. Temperature readings were taken on the CryoPod Carrier interior basket floor, 2 inches and 2.5 inches from the floor, and on the top of the basket for over 4 hours. The basket is situated above the compartment containing LN2 and there is no direct contact between samples (e.g. cryobox) and the LN2.

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Hold Time Over 4 hours < -150°C with lid closed

Capacity One 2-inch cryobox or 2-3 small cassettes

Charge Requires < 3 L liquid nitrogen (LN2)

Alarm Two settings with audible and visual signals

Lid Magnetized foam lid for safer transport and insulation

Power Battery operated front panel

Temperature Audit Trail

Downloadable temperature log data via USB port and CryoPod Data Log Software

Weight 4.1 kg (9 lbs) without LN25.9 kg (13 lbs) fully charged with 3L LN2 - no samples

External dimensions (L x W x H)

34.0 x 32.0 x 26.0 cm (13.4 x 12.6 x 10.2 in)

Cryogenic chamber basket dimensions (L x W x H)

17.4 x 18.8 x 7.8 cm (6.9 x 7.4 x 3.1 in)

Specifications Application

CryoPod™ Carrier Automatic Filling Station



CryoPod™ Carrier provides stable cryogenic sample transport and han-dling around the laboratory, campus or metro area. It accommodates one standard cryogenic storage box or 2-3 small cassettes.

• Safe; user has no interaction with LN2• Hands-free, single-button filling process• Recharges the CryoPod Carrier in less than 15 minutes• Provides precise LN2 fill every time• Bench or floor mountable

The optional CryoPod™ Carrier Automatic Filling Station offers a single-button LN2 filling solution that can be placed on a laboratory bench or floor. The Filling Station will fully charge the CryoPod Carrier within 15 minutes without the need for direct LN2 handling by the user.

Item No. DescriptionBCS-514 ● CryoPod™ Carrier, includes green lid and manual fill kitBCS-544G ● CryoPod™ lid, green, optionalBCS-544OBCS-544PK

● CryoPod™ lid, orange, optional● CryoPod™ lid, pink, optional

BCS-544GYBCS-544P

● CryoPod™ lid, grey, optional● CryoPod™ lid, purple, optional

BCS-516 CryoPod™ Manual Fill KitBCS-517 CryoPod™ LN2 Absorbent Pads, 4/pkBCS-519 CryoPod™ Foam Cryobox Guide

Item No. DescriptionBCS-515 CryoPod™ Carrier Filling Station

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BioT™ ULT Transporter provides a stable ultra-low temperature (< -70°C to -50°C) work zone 8.0 inches (20.3 cm) deep. Up to 8 standard 2-inch cryostorage boxes will remain safely below -50°C for over 8 hours with the lid open and over 24 hours with the lid closed with one charge of dry ice.

Styrofoam Box and Dry Ice: 0.6 Inches Below -50˚C BioT™ ULT Transporter: 8.0 Inches Below -50˚C

A dry ice-filled Styrofoam box is currently the standard method used for receiving, handling and transporting valuable frozen samples. However, only a 0.6 inch (1.5 cm) high zone above the dry ice stays below -50°C for 2 hours. Worse, at 2 inches (5.0 cm) above the dry ice - the top of a standard 2-inch cryostorage box- the temperature is above -20°C.

Dry Ice

0.6 inch

BioT™ ULT Transporter Ultra-Low Temperature Stability System

BioT™ ULT Transporter is a dry-ice based temperature stability system is a convenient mid-sized solution for transporting temperature-sensitive material. It also serves as a compact, portable, ultra-low freezing station for freezing samples at a collection site. The patent-pending DIR™ cooling insert technology inside the transporter harnesses cold convection for efficient, stable, and hours-long dry ice-based cooling. The base chamber accommodates up to 8 standard 2-inch cryostorage boxes. The BioT ULT Transporter LN2 Kit is an insert for the BioT™ ULT Transporter that enables the unit to be used as a transport and sorting station for samples stored at or below vapor phase liquid nitrogen temperatures. When installed in the ULT Transporter and charged with liquid nitrogen, the insert creates a cryogenic temperature zone of < -150˚C. The unit accommodates up to 4 standard 2-inch cryostorage boxes for transportation with the lid closed.

• Maintains samples between <-70°C and -50°C for over 8 hours open, 24 hours closed

• Ready to use in approximately 5 minutes• 8 inches working depth below -50°C• Lightweight, easy to carry• Intuitive assembly, durable

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BCS-518NP BioT™ ULT Transporter, magnetized lid, DIR™ dry ice retainer and base chamber

BCS-518LN BioT™ ULT Transporter LN2 Kit, thermo-conductive basket, thermo-conductive shelf plate and absorbent pad


Specifications

BioT™ ULT Transporter

Hours of <-50˚C coolingLid off: over 8 hours

Lid on: over 24 hours

Dimensions (L x W x H) Internal working area: 29.0 x 16.2 x 20.3 cm (11.4 x 6.4 x 8.0 in) External: 50.8 x 33.8 x 33 cm (20.0 x 13.3 x 13 in)

Amount of dry ice required 5.4 kg (12.8 lb)

Weight empty 3.6 kg (8.0 lb)

Weight with dry ice 9.0 kg (20.8 lb)

Working depth <-50˚C up to 20 cm (8.0 in) from chamber floor

Capacity 8 standard 2-inch cryostorage boxes

BioT™ ULT Transporter LN2 Kit

-180˚C to -150˚C at < 6’’Lid off: 1 - 1.5 hours

Lid on: 2 - 2.5 hours

Dimensions (L x W x H) Internal working area: 36.3 x 19.8 x 17.8 cm (14.3 x 7.8 x 7.0 in) External: 50.8 x 33.8 x 33 cm (20.0 x 13.3 x 13 in)

Amount of LN2 required ~2.7 L to charge unit

Weight empty 4.0 kg (8.8 lbs)

Weight with LN2 6.2 kg (13.6 lbs)

Working depth < -150°C up to 15.2 cm (6 inches) from floor plate

Capacity 4 standard 2-inch cryostorage boxes

BioT™ ULT Transporter is comprised of three parts: magnetized lid, DIR™ dry ice retainer and base chamber. To begin, insert the DIR™ retainer, flanges down, into the base chamber. Add pelletized dry ice into the cavity between the DIR retainer and the base chamber walls, filling to the top of the DIR retainer. A full load of dry ice is approximately 13 lbs (5.8 kgs). The internal chamber will reach optimal working conditions (<-70˚C to -50˚C) within approximately 10 minutes and will be ready for use.

BioT™ ULT Transporter LN2 Kit is comprised of three parts: thermo-conductive basket, thermoconductive shelf plate and absorbent/baffle pad. Simply insert the thermoconductive basket in the empty BioT ULT Transporter chamber, followed by the absorbent/baffle pad. Place the thermoconductive shelf plate into the basket on top of the absorbent/baffle pad. With the addition of LN2, (~ 2.7L) the internal chamber will reach <-150˚C within approximately 10 minutes.

How It Works

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• Large internal capacity• Patented low-noise technology• Patented lockable door and built in alarms• Unique heated door sealing and insulation system

for minimal maintenance • High energy efficiency • Unique dual evaporator design for accelerated

freezer cool down and recovery• Environmentally-friendly refrigerant

BioT™ ULT1.9 Mini Freezer is a programmable -80°C freezer with a compact footprint intended for placement on or under your lab bench. Process, freeze and store your samples within easy reach, eliminating travel time to and from community freezers. Protect your valuable samples from repeated exposure to air, residual warming due to lengthy freezer cool down and insufficient door sealing.

BioT™ ULT1.9 Mini FreezerUltra-Low Temperature -80°C Stability Freezer

Patented Sealing and Insulation Technologies Heated door seals eliminate ice formation and ensure a tight fit. Multiple door gaskets create an air-tight seal that prevents cool air from escaping, increasing energy efficiency.

Intuitive Temperature DisplayThe temperature display features intuitive programming of freezing set temperature using the up and down buttons and actual chamber temperature.

Dimensions (W x D x H) Internal: 35.0 x 44.0 x 35.5 cm (13.8 x 17.3 x 14.0 in) External: 47.0 x 62.0 x 78.0 cm (18.5 x 24.4 x 30.7 in)

Operating Range -82°C to -45°C, ±5°C -82°C to -60°C, optimal

Capacity 1.9 CuFt (54L), 2 Freezer racks with drawers

Refrigeration System Hermetically sealed cooling system

Refrigerant Environmentally-friendly, CFC/HCFC-free

CO2/LN2 Backup, optional Factory installed spare temperature sensor for external monitoring

Electrical (115V) AC105-125V, 50/60Hz

Electrical (230V) AC208-230V, 50/60Hz

Power Rating 2.1A

Fan Noise Level 50 dBA

Relative Humidity (RH) < 80% RH min, ideally <57% RH

Weight 72.0 kg (158.6 lbs)

Certifications c(ETL)us, CE

Specifications

Item No. Description BCS-805 BioT ULT1.9 Mini Freezer, 115VBCS-806 BioT ULT1.9 Mini Freezer, 230V


Temperature Display

Patented Door Sealing and Insulation

Holds up to 36 2-inch cryoboxes

Door Lock

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BioT™ Carrier 2° to 8°C Temperature Stability Chamber

BioT™ Carrier is a portable, programmable 2-8˚C cooling solution for transporting and maintaining temperature-sensitive laboratory research samples with confidence. Easily adjust the cooling temperature set point to raise or lower the chamber temperature to best suit your application. Transport cold samples between facilities, from clinic to lab, or over longer distances utilizing the car power adaptor.

Refrigeration Space (L x W x H) 18.0 x 10.0 x 8.0 cm (7.1 x 3.9 x 3.2 in)

Refrigeration Temperature 2˚C to 8˚C (ambient 32˚C)

Input/Output Voltage (power adaptor) AC100V - 240V / DC9.0V

Output Current (power adaptor) 4A

Input/Output Voltage (battery) AC100V - 240V / DC9.0V

Output Current (battery) 2A

Input/Output Voltage (car adaptor) DC12V / DC9.OV

Current (vehicle power) 4A

Voltage (lithium battery) DC 7.4V

Capacity (lithium battery) 16000mAh

Power Rating DC9.0V / 18W, DC 7.4V/13W

Weight (with battery) 5.3 kg (11.6 lbs)

Dimensions (L x W x H) Internal: 18.0 x 10.0 x 8.0 cm (7.1 x 3.9 x 3.2 in) External: 24.0 x 17.0 x 19.5 cm (9.5 x 6.7 x 7.7 in)

Certifications CE, cULus

Item No. Description BCS-528 BioT™ Carrier complete with rechargeable battery, AC power adaptor,

car adaptor, shoulder strap, accessories bagBCS-801 BioT Carrier rechargeable batteryBCS-802 BioT Carrier AC power adaptor/battery charger with US, EU, UK plugsBCS-803 BioT Carrier car adaptorBCS-804 BioT Carrier shoulder strap

Specifications Ordering Information

Intuitive LCD display

Magnetized lid

Rugged and lightweight

Rechargeable battery

Intuitive LCD DisplayThe LCD display is easy to operate. Digitally program cooling temperature set point (°C/°F) and time. Display indicates battery status and audible alarm signals when battery is low.

Flexible Power OptionsBioT™ Carrier offers flexible power options to fit your application. AC power adaptor enables long-term stationary use. Rechargeable battery is easy to install, lasts up to 8 hours and allows portable short-term transport. For applications that require longer transport, a car power adaptor is included.

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BioT™ ULT WorkstationUltra-Low Temperature (<-75° to -50°C) Stability System

BioT™ ULT Workstation is a breakthrough solution for handling and transporting valuable temperature-sensitive biomaterials, or freezing samples at collection site. The dry ice-based BioT™ ULT Workstation provides a secure ultra-low temperature (ULT) -75° to -50°C work area for processing or transporting critical frozen samples.

• Maintains < -75° to -50°C for over 15 hours• Equilibrates to < -50°C in less than 30 minutes• Insulative external housing ensures operator safety and comfort• Holds one standard 12-place freezer rack• Wheeled cart for mobility

BioT™ ULT Workstation is comprised of four parts: magnetized lid, DIR™ insert, base chamber and mobile cart.

The patent-pending DIR cooling insert technology inside the workstation harnesses cold convection for efficient, stable, and hours-long dry ice-based cooling. Pelletized dry ice is added to the cavity between the 3-sided DIR insert and base chamber walls filling to the top of the retainer (approx. 90 lbs, 41 kg). The ventilated thermo-conductive insert ensures uniform and consistent gas circulation to keep the entire base chamber at ultra-low temperature. This configuration also minimizes consumption of dry ice as one charge maintains lower than -50°C temperature for over 15 hours with the lid open.

The closed-cell cross-linked polyethylene foam construction of the lid and base chamber is durable, non-absorbent, and remains comfortable to the touch even when loaded with dry ice and frozen materials. The DIR insert is constructed of a thermo-conductive aluminum alloy anodized for durability. BioT ULT Workstation may be wiped clean with 10% bleach or 70% alcohol solutions. DIR insert may be autoclaved or wiped clean with 10% bleach or 70% alcohol solutions.

How It Works

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Performance Data

BioT™ ULT Workstation equilibrates to <-50°C in less than 30 minutes and entire chamber maintains temperature stability for over 15 hours. Temperature measured at 0 inches (blue), 4 inches (red), 7 inches (green), 10 inches (lavender).

Inch

es a

bove

cha

mbe

r floo

r

Time (hours)

Performance continued over 15 hours

Tem

pera

ture

(°C)

• Mobile cart, customizable to your working environment• RTD sensor harness and temperature display with recording

capabilities for complete chamber monitoring• Customizable to accommodate user-specified data management

equipment, software and Wi-Fi systems• Audio and visual stack light alarm system

Optional Accessories

Dimensions (L x W x H) Internal working space: 105.4 x 33.0 x 35.5 cm / 41.5 x 13.0 x 14.0 inExternal: 152.4 x 71.1 x 44.5 cm / 60.0 x 28.0 x 17.5 in

Amount of dry ice required 41 kg (90 lb)

Working depth < -50°C up to 25.4 cm (10 in)

Specifications


BioT™ ULT Workstation Performance

Mobile, versatile and ergonomic design enables ULT handling and transport for a variety of applications including:• Cherry-picking, sorting or other short term handling of critical

samples outside a -80°C freezer• Controlled-temperature transfer of frozen samples within and

between facilities• Transfer of temperature-sensitive materials to long-term storage

tanks or automated biobank facilities• Freezing of samples at collection site (in conjunction with CoolCell®

cell freezing containers or CoolRack® thermo-conductive modules)• Preparing, labeling or packaging of frozen samples for shipment

Temperature Range <-75˚ to -50˚C

Cooling Source Dry ice

Cooling Duration Open Lid Over 15 hours

Applications

Temperature and Duration


BCS-508-DI ● BioT™ ULT Workstation

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Diffuser plate

Vial module

Base vent

Lid

Chamber

Base

Top vent choke

CoolCell® Container Controlled-Rate, Alcohol-Free Cell Freezing

CoolCell® alcohol-free cell freezing containers ensure standardized controlled-rate -1oC/minute cell freezing in a -80oC freezer - without alcohol or any fluids. Proven for use with a variety of cell types including stem cells, primary cells, PBMC cell lines, insect cells, yeast and others. The patent-pending CoolCell technology utilizes a thermo-conductive alloy core and highly-insulative outer material to control the rate of heat removal and provide reproducible cell cryopreservation. CoolCell units are easy to use and deliver comparable results to expensive programmable freezers.

CoolCell® Container Isopropanol (IPA) Container

No alcohol • No fluids• No pre-cooling • Saves 12L/unit of IPA per year

Requires isopropanol• Replace alcohol every 5 uses• Track number of uses• Pre-cool alcohol in refrigerator

No variability• All vials have uniform freeze rate• Radially symmetric design ensures

vial consistency

Inconsistent freeze rate• Alcohol degradation induces

variability• Two circles of wells; two freeze rates

No on-going cost• No alcohol purchase or disposal

Approximately $350/year• Change alcohol weekly • Dispose of hazardous waste

No stuck lids• Ergonomic lid comes off easily when

frozen• Not cold to the touch when removing

from the -80˚C freezer

Difficult to handle and open• Screw cap difficult to remove when

frozen• Frozen unit is slippery and cold to

touchQuick re-use time• Ready to use again after five minutes

Wait between runs• Takes >1 hr for alcohol to warm-up

CoolCell® FTS30 utilizes a solid state core and controlled micro-convection technology to evenly draw in -80°C freezer air through a bottom base vent, uniformly disperse the cold air around each vial in the central chamber and then release the thermal load from the vials through a top vent choke. The inner vial module holds 30 cryogenic vials and can be removed in one step. Each vial achieves a uniform and reproducible -1°C/minute freezing profile and thermal profiles are highly reproducible. Due to the low thermal mass of the uniform-density cross-linked polyethylene foam container, freezing can be conducted without a rise in local freezer temperature, thereby protecting nearby samples.

CoolCell® LX, in combination with a -80°C freezer, will provide the freezing rate of -1°C/minute that is ideal for cryopreservation of most cells and cell lines. Using a combination of uniform-density cross-linked polyethylene foam, a solid state core, and radial vial symmetry, freezing profiles are consistent and reproducible. It is important to fully load CoolCell containers prior to freezing. Foam is non-absorbent and will impose negligible change in the freezer environment; thereby protecting nearby frozen samples. The low heat content also ensures that CoolCell LX containers will rapidly return to room temperature when removed from freezer.

Chamber

Core

Base

Stem CellsHuman Embryonic Stem Preadipocytes Breast Cancer Stem Colon Cancer Stem Glioblastoma Stem Mouse Embryonic Stem Human Endothelial Progenitor

Primary CellsNeonatal Keratinocytes Human WBCs Mouse WBCs Human CD34+ Muscle Human Tendon Fibroblasts Melanoma Tumor Human Cardiac Ventricular Human Cardiac AtrialCell Lines

CHO LnCap HTB77 A549 HeLa

How It Works

Lid

CoolCell® is proven to work with many cell types including:

16

Performance test: A temperature probe was placed into a 2.0 mL cryogenic vial containing 1.0 mL of water and the tube was inserted into a room temperature CoolCell®. The CoolCell was placed into a -80°C freezer and the temperature rate and profile was recorded over a 3 hour period. The test was repeated 5 consecutive times. Conclusion: CoolCell generated identical fusion time and cooling profiles over five consecutive freeze cycles.

CoolCell® Reproducibility CoolCell® Protocols

CoolCell® Performance vs. IPA Container

CoolCell® Performance vs. Programmable Freezer

Alamar blue reduction assay for proliferation assessment showed cells frozen in CoolCell® grew more quickly, leading to more total cells.

Human embryonic stem cells, RC-10 were frozen using the technique indicated, thawed after 2 weeks in LN2, and counted immediately (Day 1) or after 3 days of growth (Day 3).

Ag-Tregs. Effects of freezing on antigen-specific Treg (Ag-Treg) cell therapy products; Ag-Tregs (n = 6) were frozen at concentration of 1 to 10 x 106 cells/mL using the CoolCell freezing device or controlled-rate freezer (CRF) with a freezing rate of -1°C/min. Viability and absolute viable cell count of thawed Ag-Treg cell therapy products were evaluated by flow cytometry.

- Data generated by TxCell SA

Comparison of freezing methods. Graph comparing % of cell recovery after freezing with the CoolCell freezing device (blue) versus freezing using a programmable step-down freezer (red) in 3 different samples at high cell concentration. There was no significant difference between the two freezing methods.

- Data performed by UCSF Diabetes Center

6 hours

24 hoursDay 3

Day 1

HeLA, CHO-K, K562, NIH3T3. CoolCell® 12-well, CoolCell FTS30 30-well or “Mr. Frosty” freezing containers were used to freeze all four cell lines. Identical transfection efficiencies and viabilities were observed after thawing.

Identical growth of cells was observed 24 hours post-thaw.

Mr. Frosty

CoolCell (12 samples)


Mr. Frosty



% C

ell R

ecov

ery

Cryopreservation and Thawing of Cells

Wayne M. Yokoyama, Maria L. Thompson, Rolf O. EhrhardtUniversity of California School of Medicine, San Francisco, CA BioCision LLC, Larkspur, CaliforniaCurr. Protoc. Immunology. 2012 Nov; 99 Appendix 3G

Standardized Cryopreservation of Pluripotent Stem Cells

Rick I. Cohen, Maria L. Thompson, Brian Schryver, Rolf O. EhrhardtRutgers University, Piscataway, New JerseyBioCision LLC, San Rafael, CaliforniaCurr. Protoc. Stem Cell Biol. 28:1C.14.1-1C.14.10

Standardized Cryopreservation of Human Primary Cells

Thomas V. Ramos, Aby J. Mathew, Maria L. Thompson, Rolf O. EhrhardtHemaCare Corporation, Van Nuys, California, BioLife Solutions, Bothell, Washington, BioCision, Larkspur, CaliforniaCurr. Protoc. Cell Biology. 2014 Sept; 64 Appendix 3I.

Growth Performance 24 hours Post-thaw% Viability

17

CoolCell® LX For 12 standard 1.0 mL to 2.0 mL cryogenic vials, 1.0 mL fill per vial. Radially symmetric for uniform vial freezing. Numbered wells for easy sample identification. Beveled lid for secure gripping and easy open-ing. Exposed vial tops when lid is open for quick, organized removal of frozen samples.

Item No. Description External Dimensions Well Diam.BCS-405 ● CoolCell LX, purple Diam 11.7 x H 9.9 cm 12.7 mmBCS-405G ● CoolCell LX, greenBCS-405O ● CoolCell LX, orangeBCS-405PK ● CoolCell LX, pink

CoolCell® SV2 and CoolCell® SV10 For 12 standard 2.0 mL injectable ampules, 1.0 mL fill per ampule (SV2). For 6 standard 10.0 mL injectable ampules, 5.0 mL fill per ampule (SV10). Radially symmetric for uniform freezing of injectable ampules. Easy open lid. Exposed vial tops when lid is open for quick, organized removal of frozen samples.

CoolCell® FTS30 For 30 standard 1.0 mL to 2.0 mL cryogenic vials, 1.0 mL fill per vial. Controlled micro-convection for uniform freezing of 30 vials. Removable vial tray for one-step transfer of samples into and out of freezing chamber.

For 1.0 mL or 2.0 mL Cryogenic Vials

For 3.5 mL to 5.0 mL Cryogenic Vials For Injectable Cell Therapy Ampules

Item No. Description External Dimensions Well Diam. BCS-170 ● CoolCell FTS30, purple Diam 16.5 x H 11.5 cm 12.3 mmBCS-170G ● CoolCell FTS30, greenBCS-170O BCS-170PK

● CoolCell FTS30, orange ● CoolCell FTS30, pink

Item No. Description External Dimensions Well Diam. BCS-406 ● CoolCell 5ml LX, purple Diam 9.5 x H 14.5 cm 15.2 mm

CoolCell® 5mL LX For 12 standard 3.5 mL to 5.0 mL fill cryogenic vials, 3.5 to 5.0 mL fill per vial. Radially symmetric for uniform vial freezing. Numbered wells for easy sample identification. Beveled lid for secure gripping and easy opening. Exposed vial tops when lid is open for quick, organized removal of frozen samples.

Item No. Description External Dimensions Well Diam.BCS-172 ● CoolCell SV2, purple Diam 13.9 x H 10.5 cm 14.7 mmBCS-262 ● CoolCell SV10, purple Diam 12.1 x H 9.8 cm 23.6 mm

Item No. Description For Use WithBCS-210 Removable cryogenic vial module

for CoolCell FTS30CoolCell FTS30

Note: For optimal freezing it is important to fully load each CoolCell container prior to freezing. CoolCell Filler Vials are recommended for filling any empty wells.

Item No. Description For Use WithBCS-3105 CoolCell Filler Vial, 2ml CoolCell LX, CoolCell FTS30BCS-3106 CoolCell Filler Vial, 5ml CoolCell 5ml LX

CoolCell® Filler Vials To ensure cell freezing rate consistency and uniform results when using CoolCell containers, insert a CoolCell Filler Vial into empty wells when freezing less than a full load. Suitable for repeated use and compatible with CoolCell LX, CoolCell FTS30 and CoolCell 5ml LX containers. 6 per pack.

CoolCell® FTS30 Vial Module FTS30 Vial Module is a holder for 30 1.0 mL or 2.0 mL cryogenic vials that allows one-step insertion and removal of all 30 vials at once. Fits into a standard 5.0 x 5.0 x 2.0 inch cryostorage box. Compatible with dry ice and liquid nitrogen.

18

TruCool® Hinged CryoBoxes Patented hinged lid offers convenience and archival integrity, ensuring markings and vials remain in sync. Lid stays attached to base minimizing risk of separation and lid contamination. Lid is easy to open when frozen. Available in 9x9,10x10, and vapor phase LN2 compatible formats. Plastic 81-place grid has adjustable slats to accommodate multiple vial types. 2-inch box holds 1.0 mL or 2.0 mL cryogenic vials and microfuge tubes. 3.5-inch box holds 3.0 mL to 5.0 mL cryogenic vials.

* Ordering Information on page 29 - 30

Cell Cryopreservation Systems

Cell Cryopreservation Accessories*

CryoCeps™ Cryogenic Vial Grippers Cryogenic vial grippers feature a unique design to grasp internal- or external-thread cryogenic vials. Easily sort or move vials while maintaining sterility and protecting fingers from frozen vials, dry ice and liquid nitrogen. 5 per pack.

TruCool® Barcoded Cryogenic Vials TruCool leak-proof, auto-cap cryogenic vials are ideal for cell culture and biobanking. The screw cap features a co-molded thermally-fused gasket which prevents leaking, slipping and risk of contamination. The gasket is 95kPa certified to provide a leak-proof seal. The star socket on cap top is compatible with auto-decapping equipment. Each vial is individually barcoded with a unique identifier that can be read with common barcode readers. Not recommended for storage directly in liquid nitrogen. Vapor phase ok. 500 per case. Internal Threads External Threads

CoolCell® SV2 Stem Cell Cryopreservation System

Item No. Description For Use WithBCS-172CS ● CoolCell SV2 Stem Cell

Cryopreservation SystemKit designed for efficient and controlled preparation and cryopreservation of stem cells and cell therapy products. For 2.0 mL injectable cell therapy ampules.

CoolCell® SV10 Stem Cell Cryopreservation System

Item No. Description For Use WithBCS-262CS ● CoolCell SV10 Stem Cell

Cryopreservation SystemKit designed for efficient and controlled preparation and cryopreservation of stem cells and cell therapy products. For 10.0 mL injectable cell therapy ampules.

19

CoolBox™ Systems Ice-Free Cooling and Freezing

CoolBox™ ice-free systems are bench top cooling workstations that provide sample cooling or freezing without ice, electricity or batteries. CoolBox systems are versatile and accommodate a variety of sample formats and temperatures. The modular design enables the use of CoolRack® and CoolSink® thermo-conductive sample modules to hold microfuge tubes, cryogenic vials, PCR tubes or plates, assay plates and more.

How It Works

• Freeze the XT Cooling Core in -20°C freezer

• Place frozen core in base

• Rest CoolRack or CoolSink module on core

• Module will equilibrate and maintain temperature

via thermo-conductivity

Lid

Collar

Base

Pre-frozen XT Cooling Core(s)

CoolRack® or CoolSink® plate module

1. Identify Tube or Plate 2. Choose CoolRack® orCoolSink® Module

3. Choose CoolBox™ capacity and color

Examp le 1 up to 24 microcentrifuge tubes

CoolRack® XT M24 CoolBox™ XT

Examp le 2 up to 48 microcentrifuge tubes

2 x CoolRack® XT M24 CoolBox™ 2XT

Examp le 324 microcentrifuge tubes and one PCR plate, 12 PCR strips,

or 0.2 mL PCR tubes CoolRack® XT M24 + CoolRack® XT PCR96

CoolBox™ 2XT

How to Configure a CoolBox™ Ice-Free System

20

For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No. Capacity

1.5 mL or 2.0 mL tubes 6 Cylindrical 6.0 x 4.3 x 3.8 cm 11.1 mm 32.7 mm CoolRack M6 BCS-163 1 up to 3 up to 8 up to 2

1.5 mL or 2.0 mL tubes 15 Cylindrical 10.2 x 6.4 x 3.8 cm 11.1 mm 32.7 mm CoolRack M15 BCS-125 1 1 up to 4 1

1.5 mL conical tubes 15 Conical 10.2 x 6.4 x 3.8 cm 11.1 mm 35.3 mm CoolRack M15-PF BCS-127 1 1 up to 4 1

1.5 mL 0r 2.0 mL tubes 24 Cylindrical 12.8 x 8.5 x 3.8 cm 11.1 mm 32.7 mm CoolRack XT M24* BCS-535 1 1 up to 2 --

5.0 mL centrifuge tubes 12 Conical 12.7 x 8.6 x 5.0 cm 16.5 mm 48.7 mm CoolRack XT 5ml* BCS-539 1 1 up to 2 --

1.5 mL or 2.0 mL tubes 30 Cylindrical 12.0 x 10.2 x 3.8 cm 11.1 mm 32.7 mm CoolRack M30 BCS-108 1 -- up to 2 1

1.5 mL conical tubes 30 Conical 12.0 x 10.2 x 3.8 cm 11.1 mm 35.3 mm CoolRack M30-PF BCS-128 1 -- up to 2 1

500 uL conical tubes 30 Conical 12.0 x 10.2 x 3.8 cm 11.1 mm 35.3 mm CoolRack M30-PF 500ul BCS-137 1 -- up to 2 1

Cryogenic vial and FACS tube modules: CoolRack® CFFor Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No.

cryogenic vials or FACS tubes

15 Cylindrical 10.2 x 6.4 x 3.8 cm 12.7 mm 32.7 mm CoolRack CF15 BCS-126 1 1 up to 4 1


24 Cylindrical 12.8 x 8.5 x 3.8 cm 12.7 mm 32.7 mm CoolRack XT CFT24* BCS-534 1 1 up to 2 --


30 Cylindrical 12.0 x 10.2 x 3.8 cm 12.7 mm 32.7 mm CoolRack CFT30 ø BCS-138 1 -- up to 2 1


45 Cylindrical 17.3 x 9.7 x 3.8 cm 12.7 mm 32.7 mm CoolRack CF45 BCS-105 -- -- 1 --

PCR plate, strip well or tube modules: CoolRack® PCRFor Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No.

One 96-well PCR plate, strip wells, 0.2mL tubes

96 Tapered 12.7 x 8.6 x 2.5 cm - 13.2 mm CoolRack XT PCR96* BCS-529 1 1 up to 2 --

6 strip wells and 12 x 1.5 or 2.0 mL microfuge tubes

48(PCR) 12(M)

Tapered(PCR) Cylindrical(M)

12.7 x 8.6 x 3.8 cm -11.1 mm

13.2 mm32.7 mm

CoolRack XT M-PCR* BCS-523 1 1 up to 2 --

One 384-well PCR plate 384 Tapered 12.7 x 8.6 x 1.9 cm - 7.6 mm CoolRack XT PCR384* BCS-538 1 1 up to 2 --

2D barcode storage tube modules: CoolRack® 96For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No.

0.5 mL 2D storage tubes

96 Cylindrical 13.1 x 8.9 x 3.6 cm 8.4 mm 24.6 mm CoolRack 96x0.5ml BCS-231 1 1 up to 2 --

1.4 mL 2D storage tubes

96 Cylindrical 13.2 x 8.9 x 3.6 cm 8.3 mm 32.7 mm CoolRack 96x1ml BCS-149 1 1 up to 2 --

Cell therapy injectable ampule modules: CoolRack® SVFor Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No.

2.0 mL injectable cell therapy ampules

12 Cylindrical 12.7 x 8.6 x 3.8 cm 16.0 mm 24.0 mm CoolRack SV2 BCS-266 1 1 up to 2 --

10.0 mL injectable cell therapy ampules

12 Cylindrical 12.7 x 8.6 x 3.8 cm 23.6 mm 27.9 mm CoolRack SV10 BCS-265 1 1 up to 2 --

Tall tube modules: CoolRack® 15ml, CoolRack® 50ml, CoolRack® 250mlFor Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No.

15 mL centrifuge tubes 12 Cylindrical 13.7 x 9.5 x 11.8 cm 17.5 mm 105.4 mm CoolRack Lt BCS-232 1 1π up to 2π --

15 mL centrifuge tubes 9 Cylindrical 8.9 x 8.9 x 10.7 cm 17.1 mm 106.7 mm CoolRack 15ml BCS-153 1 1π up to 2π --

50 mL centrifuge tubes 4 Cylindrical 8.9 x 8.9 x 10.7 cm 29.5 mm 101.6 mm CoolRack 50ml BCS-154 1 1π up to 2π --

250 mL centrifuge tube 1 Conical 8.9 x 8.9 x 14.0 cm 60.4 mm 133.3 mm CoolRack 250ml-PF BCS-532 1 1** up to 2** --

250 mL centrifuge tube 1 Cylindrical 8.9 x 8.9 x 7.2 cm 73.6 mm 66. mm n/a BCS-533 1 1 up to 2 --

Blood collection tube modules: CoolRack® V For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Description Item No.

13 mm or 16 mm blood tubes

12 Cylindrical 13.7 x 9.5 x 9.6 cm 16.6 mm 83.3 mm CoolRack LVt BCS-235 1 1 up to 2π --

13x75 mm blood tubes 9 Cylindrical 8.9 x 8.9 x 6.1 cm 13.0 mm 61.0 mm CoolRack VS13 BCS-157 1 1π up to 2π --

13x100 mm blood tubes or 5 mL cryogenic vials

9 Cylindrical 8.9 x 8.9 x 8.4 cm 13.0 mm 83.8 mm CoolRack V13 BCS-155 1 1π up to 2π --

16x100 mm blood tubes

9 Cylindrical 8.9 x 8.9 x 8.4 cm 16.0 mm 83.8 mm CoolRack V16 BCS-156 1 1π up to 2π --

*SBS-compatible ø “gripping” wells for one-hand vial opening/closing tThermo-conductive base and insulative exterior π Requires extension collar accessory for closed lid cooling ** Lid closure not possible even with the addition of extension collar

Choose Your CoolRack® Module

Microfuge tube modules: CoolRack® M

XT Starter CoolBox XT CoolBox 2XT CoolBox 30

Open 4 hr 10 hr 10 hr 4 hr

Closed -- 16 hr 16 hr 10 hr

21

XT Starter

Choose Your CoolBox™ System

An open-platform ice-free cooler that accommodates most CoolRack® and CoolSink® modules. Low profile and small footprint make it ideal for use in the hood, keeping samples cold (0.5° to 4.0°C) up to four hours. 1°C to 8°C temperature indicator provides visual assurance of temperature performance. To extend the cooling duration, keep an additional XT Cooling Core in the freezer and rotate the Cores as needed.

Item No. Description Dimensions (L x W x H)BCS-504 ● XT Starter, complete, purple External: 16.2 x 12.1 x 6.1 cmBCS-513 ● XT Starter Holder only, purpleBCS-511 XT Cooling Core only

Item No. Description Dimensions (L x W x H)BCS-502 ● CoolBox XT, purple Internal: 14.0 x 9.4 x 9.9 cm*

External: 20.0 x 16.0 x 15.0 cmBCS-502G ● CoolBox XT, greenBCS-502O ● CoolBox XT, orangeBCS-502PK ● CoolBox XT, pink

*Internal height of open space when core is in the base.

CoolBox™ XT SystemIncludes: CoolBox XT base, collar, lid and (1) XT Cooling Core for 0.5° to 4°C cooling.

CoolBox™ XT or CoolBox 2XT System

Keep sample tubes or plates cold for over 16 hours with the lid on, and over 10 hours with the lid off. Use optional XT Freezing Core to maintain frozen samples for over 8 hours. Dry ice may be used in place of the cores to create a compact snap freezing workstation.

Item No. Description Dimensions (L x W x H)BCS-503 ● CoolBox 2XT, purple Internal: 21.0 x 14.3 x 13.4 cm*

External: 26.5 x 20.0 x 15.0 cm BCS-503G ● CoolBox 2XT, greenBCS-503O ● CoolBox 2XT, orangeBCS-503PK ● CoolBox 2XT, pink

*Internal height of open space when core is in the base.

CoolBox™ 2XT SystemIncludes: CoolBox 2XT base, collar, lid and (2) XT Cooling Cores for 0.5° to 4°C cooling.

Item No. Description Dimensions (L x W x H):BCS-502-C ● XT Extension Collar, purple Internal: 14.0 x 9.4 x 7.0 cm

External: 20.0 x 16.0 x 7.0 cmBCS-502-CG ● XT Extension Collar, greenBCS-502-CO ● XT Extension Collar, orangeBCS-502-CPK ● XT Extension Collar, pink

BCS-503-C ● 2XT Extension Collar, purple Internal: 21.0 x 14.3 x 7.0 cm External: 26.5 x 20.0 x 7.0 cmBCS-503-CG ● 2XT Extension Collar, green

BCS-503-CO ● 2XT Extension Collar, orangeBCS-503-CPK ● 2XT Extension Collar, pink

XT and 2XT Extension CollarFor use with CoolBox XT and CoolBox 2XT systems to accommodate tall tube modules. The collar is magnetized and easily adheres to the unit base.

Item No. DescriptionBCS-511 XT Cooling Core, 0.5° to 4°CBCS-512 XT Freezing Core, below 0°C

Optional Accessories

XT CoresKeep additional cooling or freezing cores in the freezer for flexibility and extended duration. XT Cooling Core features a 1 to 8°C temperature indicator. Both cooling and freezing cores feature a thermo-conductive surface for uniform temperature distribution.

Temperature indicator!

XT Starter CoolBox XT & 2XT CoolBox 30

Holds Tubes Yes Yes Yes

Holds Plates Yes Yes n/a

0.5° - 4°C cooling with lid open 4 hours 10 hours 4 hours

0.5° - 4°C cooling with lid closed n/a 16 hours 10 hours

<0°C freezing with lid open n/a 5 hours 3 hours

<0°C freezing with lid closed n/a 8 hours 6 hours

22

Popular Pre-assembled Configurations

Application For Use With Includes Description Item No. ColorEnzyme, PCR reagent or other biological specimen cooling

24 x 1.5 mL or 2.0 mL microfuge tubes 1 x CoolBox XT System1 x SBS-compatible CoolRack XT M24

CoolBox XT MicroTube 24 System

BCS-576●

Cell thawing, tissue culture 24 x 1.0 mL or 2.0 mL cryogenic vials with “gripping” wells for one-hand tube opening/closing

1 x CoolBox XT System1 x SBS-compatible CoolRack XT CFT24

CoolBox XT CryoTube 24 System

BCS-575●

PCR/qPCR plate preparation One 96-well PCR plate, 12 strip wells or 96 0.2 mL tubes

1 x CoolBox XT System1 x SBS-compatible CoolRack XT PCR96

CoolBox XT PCR96 System

BCS-570●

PCR/qPCR strip well and reagent tube preparation

6 strip wells and 12 microfuge tubes (1.5 to 2.0 mL)

1 x CoolBox XT System1 x SBS-compatible CoolRack XT M-PCR

CoolBox XT PCR Strip System

BCS-572●

PCR/qPCR plate and reagent tube preparation

One 96-well PCR plate 12, strip wells 96 0.2 mL tubes and 24 x 1.5 mL or 2.0 mL microfuge tubes

1 x CoolBox 2XT System1 x SBS-compatible CoolRack XT M24 1 x CoolRack PCR96

CoolBox 2XT PCR Sample Preparation System

BCS-573

●

Application For Use With Includes Description Item No. ColorPCR/qPCR plate and reagent tube preparation

One 96-well PCR plate, 12 strip-wells or 96 0.2 mL tubes

1 x XT Starter 1 x CoolRack XT PCR96

XT Starter PCR96 System

BCS-556●

PCR/qPCR strip well and reagent tube preparation

12 x 1.5 mL or 2.0 mL microfuge tubes and 6 rows of PCR stripwells

1 x XT Starter 1 x CoolRack XT M-PCR

XT Starter M-PCR System

BCS-557●

Application For Use With Includes Description Item No. ColorEnzyme, PCR reagent or other biological specimen cooling

30 x 1.5 mL or 2.0 mL microfuge tubes 1 x CoolBox 30 System1 x CoolRack M30

CoolBox M30 System BCS-133●

Cell preparation or thawing, tissue culture

30 x 1.0 mL or 2.0 mL cryogenic vials, with "gripping" wells for one-hand vial opening and closing

1 x CoolBox 30 System1 x CoolRack CFT30

CoolBox CFT30 System BCS-166●

CoolBox™ 30 SystemKeeps tubes cold (0.5° to 4.0°C) for up to 10 hours. Use the optional freezing cartridge to maintain frozen samples below 0°C for up to 6 hours.

CoolBox 30 CartridgesItem No. DescriptionBCS-132 Cooling Cartridge, blue, 3 per packBCS-131 Freezing Cartridge, green, 3 per pack

Item No. Description Dimensions (L x W x H)BCS-130 ● CoolBox 30, purple Internal: 12 x 10.4 x 7.9 cm*

External: 16.5 x 15.25 x 12.7 cm*Internal height of open space when cartridge is in the base.

CoolBox™ 30 SystemIncludes: CoolBox 30 base and lid, blue cooling cartridge.

XT Starter PCR Cooling Systems, pre-assembled

CoolBox™ XT and CoolBox™ 2XT Systems, pre-assembled

CoolBox™ 30 Systems, pre-assembled

23

CoolRack® Thermo-Conductive Tube Modules

CoolRack® thermo-conductive tube modules eliminate variability which originates from tubes placed directly into ice, dry ice, alcohol baths, water baths and other temperature sources. Place the CoolRack module directly onto a temperature source between -196°C to >100°C and it will rapidly adapt to that temperature. CoolRack modules ensure +/- 0.1°C temperature uniformity across all tubes when cooling, snap freezing, heating or thawing. Suggest-ed applications include cooling reagents such as restriction enzymes, dNTPs and antibodies, alcohol-free dry ice snap freezing of tissue, virus and bac-teria samples and bench top cryogenic tube sorting in liquid nitrogen. All CoolRack modules may be autoclaved, high heat sterilized or decontaminated with bleach, alcohol or other disinfectants or lab detergents. Certain CoolRack modules are SBS-compatible.

Solution: Samples in CoolRack® Module• All samples <4°C and uniform in

temperature (+/- 0.1°C)• Samples organized, secure and dry• All tubes upright and indexed• Reproducible method

Problem: Samples in Ice• Non-uniform ice contact results in

variable sample temperature• Disorganized samples, wet labels• Shifting, sinking tubes;

contamination risk• Non-reproducible method

How It Works

Optimized well spacing

Rapidly adapts to desired temperature

Thermo-conductive alloy module

Autoclavable

Cylindrical or conical well shapes

Recessed for easy grip

CoolRack in Water Bath: Heat is transferred from water bath toward relatively cooler CoolRack until equilibrium is reached.

CoolRack® and CoolSink® are precision-engineered sample modules manufactured from a novel thermo-conductive alloy material. Thermo-conductivity is the transfer of heat energy from a higher temperature region to a lower temperature region. CoolRack modules evenly distribute the temperature across all wells providing very uniform and consistent temperature to all samples (+/0.1°C).

Cooling

Warming

CoolRack on Ice: Heat from the relatively warmer CoolRack module is transferred to cooling source (wet or dry ice, cartridge, LN2) until equilibrium is reached.

24

CoolRack® Versatility and Performance

On Ice• Adapts from ambient (25˚C) to <4˚C in 60-90

seconds*

• Samples and labels stay dry, organized• Hours of ice cooling without direct ice contact• Reproducible method

On Dry Ice• Adapts from ambient (25˚C) to -78˚C in

approximately 5-7 minutes*

• Eliminates ethanol from snap freezing • Samples are upright and organized as they freeze• Equal or better freezing rate as compared to

direct immersion into dry ice or alcohol slurry• Reproducible method

In Liquid Nitrogen (LN2)• Adapts from ambient (25˚C) to approximately -150˚C

in approximately 12-14 minutes*

• Vapor barrier protects from ambient air• Samples are upright and organized as they freeze• No direct contact between samples and LN2• Reproducible method

*Average cooling rate from room temperature

With Heat Sources• Use with water baths, hot plates, incubators

and other heat sources to keep samples warm

Performance test: A temperature probe was placed into a 2.0 mL cryogenic vial containing 1.0 mL of water. The tube was inserted into a CoolRack® CF45 thermo-conductive module. The module was placed onto a ThermalTray platform in a 37°C water bath and allowed to equilibrate. The CoolRack CF45 module was then removed and placed onto dry ice and equilibrated to -78°C (0 - 15 minutes) and then returned to the water bath to re-equilibrate to 37°C (15 - 30 minutes). This experiment was repeated five consecutive times and temperature profiles were recorded.

Conclusion: The CoolRack CF45 module showed identical cooling profiles and phase transition (orange circles) over five consecutive freeze-thaw cycles.

CoolRack® Reproducibility

LN2 Refill to 5 cm

25

CoolRack® Sample Tube Modules

CoolRack® M microfuge tube modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-163 BCS-165BCS-164

CoolRack M6CoolRack M6, greenCoolRack M6, orange

1.5 mL or 2.0 mL tubes 6 Cylindrical 6.0 x 4.3 x 3.8 cm 11.1 mm 32.7 mm 17.8 mm 17.8 mm

BCS-125BCS-125GBCS-125O



BCS-127 CoolRack M15-PF 1.5 mL conical tubes 15 Conical 10.2 x 6.4 x 3.8 cm 11.1 mm 35.3 mm 17.8 mm 17.8 mm

BCS-535 CoolRack XT M24* 1.5 mL 0r 2.0 mL tubes 24 Cylindrical 12.8 x 8.5 x 3.8 cm 11.1 mm 32.7 mm 17.8 mm 17.8 mm

BCS-539 CoolRack XT 5ml* 5.0 mL centrifuge tubes 12 Conical 12.7 x 8.6 x 5.0 cm 16.5 mm 48.7 mm 28.0 mm 28.0 mm

BCS-108 BCS-108G BCS-108O



BCS-128 CoolRack M30-PF 1.5 mL conical tubes 30 Conical 12.0 x 10.2 x 3.8 cm 11.1 mm 35.3 mm 17.8 mm 17.8 mmBCS-137 CoolRack M30-PF 500ul 500 uL conical tubes 30 Conical 12.0 x 10.2 x 3.8 cm 11.1 mm 35.3 mm 17.8 mm 17.8 mm

BCS-102 CoolRack M90 1.5 mL or 2.0 mL tubes 90 Cylindrical 26.8 x 11.2 x 3.8 cm 11.1 mm 32.7 mm 17.8 mm 17.8 mm

BCS-116 CoolRack M96IDö 1.5 mL or 2.0 mL tubes 96 Cylindrical 25.4 x 15.2 x 3.8 cm 11.1 mm 32.7 mm 17.8 mm 17.8 mm

*SBS-compatible öCoolRack M96ID has A-H and 1-12 row and column indexing

CoolRack® CF cryogenic vial and FACS tube modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-126 CoolRack CF15 cryogenic vials or FACS

tubes15 Cylindrical 10.2 x 6.4 x 3.8 cm 12.7 mm 32.7 mm 17.8 mm 17.8 mm

BCS-534 CoolRack XT CFT24*ö cryogenic vials or FACS tubes

24 Cylindrical 12.8 x 8.5 x 3.8 cm 12.7 mm 32.7 mm 17.8 mm 17.8 mm

BCS-138 CoolRack CFT30ö cryogenic vials or FACS tubes


BCS-105 CoolRack CF45 cryogenic vials or FACS tubes


*SBS-compatible ö “gripping” wells for one-hand vial opening/closing

CoolRack® PCR plate, strip well or tube modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-529 CoolRack XT PCR96* One 96-well PCR plate, strip

wells, tubes96 Tapered 12.7 x 8.6 x 2.5 cm - 13.2 mm 9.0 mm 9.0 mm

BCS-523 CoolRack XT M-PCR* 6 strip wells and 12 x 1.5 or 2.0 mL microfuge tubes

48(PCR)

12(M)

Tapered(PCR) Cylindrical(M)

12.7 x 8.6 x 3.8 cm - 11.1 mm

13.2 mm32.7 mm

9.0 mm14.0 mm

9.0 mm20.3 mm

BCS-538 CoolRack XT PCR384* One 384-well PCR plate 384 Tapered 12.7 x 8.6 x 1.9 cm - 7.6 mm 4.5 mm 4.5 mm*SBS-compatible

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CoolRack® 15ml and CoolRack® 50ml tube modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-232 CoolRack L* 15 mL centrifuge tubes 12 Cylindrical 13.7 x 9.5 x 11.8 cm 17.5 mm 105.4 mm 25.4 mm 30.5 mmBCS-153 CoolRack 15ml 15 mL centrifuge tubes 9 Cylindrical 8.9 x 8.9 x 10.7 cm 17.1 mm 106.7 mm 26.7 mm 26.7 mmBCS-154 CoolRack 50ml 50 mL centrifuge tubes 4 Cylindrical 8.9 x 8.9 x 10.7 cm 29.5 mm 101.6 mm 40.6 mm 40.6 mm

BCS-532 CoolRack 250ml-PF 250 mL centrifuge tube 1 Conical 8.9 x 8.9 x 14.0 cm 60.4 mm 133.3 mm n/a n/aBCS-533 CoolRack 250ml 250 mL centrifuge tube 1 Cylindrical 8.9 8.9 x 7.2 cm 73.6 mm 66.5 mm n/a n/a* Thermo-conductive base and insulative exterior

CoolRack® SV cell therapy injectable ampule modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-266 CoolRack SV2 2.0 mL injectable cell therapy

ampules12 Cylindrical 12.7 x 8.6 x 3.8 cm 16.0 mm 24.0 mm 27.0 mm 30.0 mm

BCS-265 CoolRack SV10 10.0 mL injectable cell therapy ampules


Tall Tube Modules

CoolRack® 96 2D barcode storage tube modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-231 CoolRack 96x0.5ml 0.5 mL 2D storage tubes 96 Cylindrical 13.1 x 8.9 x 3.6 cm 8.4 mm 24.6 mm 9.0 mm 9.0 mm

BCS-149 CoolRack 96x1ml 1.4 mL 2D storage tubes 96 Cylindrical 13.2 x 8.9 x 3.6 cm 8.3 mm 32.7 mm 14.0 mm 17.8 mm

CoolRack® V blood collection tube modules

Item No. Description For Use With Wells Well Shape Dimensions (L x W x H) Well Dia. Well Depth Row Spacing Column SpacingBCS-235 CoolRack LV* 13 mm or 16 mm blood tubes 12 Cylindrical 13.7 x 9.5 x 9.6 cm 16.6 mm 83.3 mm 25.4 mm 30.5 mm

BCS-157 CoolRack VS13 13x75 mm blood tubes 9 Cylindrical 8.9 x 8.9 x 6.1 cm 13.0 mm 61.0 mm 26.7 mm 26.7 mm

BCS-155 CoolRack V13 13x100 mm blood tubes or 5 mL cryogenic vials


BCS-156 CoolRack V16 16x100 mm blood tubes 9 Cylindrical 8.9 x 8.9 x 8.4 cm 16.0 mm 83.8 mm 26.7 mm 26.7 mm* Thermo-conductive base and insulative exterior

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Problem: Non-Uniform Plate Cooling with Crushed IceFinal equilibrium well temperature for a 96-well flat bottom plate in direct contact with crushed ice.

CoolSink® Thermo-Conductive Plate Modules

CoolSink® thermo-conductive plate and reservoir modules provide uniform temperature to all wells, regardless of position. When placed onto a temperature source such as ice, dry ice, liquid nitrogen or water baths, the CoolSink module will rapidly adapt to that temperature - from -196°C to >+100°C. CoolSink modules ensure temperature sample uniformity when cooling, snap freezing, heating or thawing samples. All CoolSink modules may be autoclaved, high heat sterilized or decontaminated with bleach, alcohol or other disinfectants or lab detergents. All modules are compatible with all temperature sources.

Solution: Uniform Plate Cooling with CoolSink® XT 96F ModuleCoolSink 96F on ice. Colors represent 0.5°C temperature intervals of the corresponding plate wells. The white cell represents the well that was fitted with the thermocouple probe.

Item No. Description For Use With Dimensions (L x W x H)BCS-536 CoolSink XT 96F For one 6-, 12-, 24-,

48-, 96- or 384-well flat bottom plate

12.8 x 8.5 x 1.7 cm

BCS-537 CoolSink XT 96U For one 96-well u-bottom plate

12.8 x 8.5 x 1.7 cm

Item No. Description Dimensions (L x W x H)BCS-184 CoolSink LX55 14.6 x 6.4 x 3.5 cm

Plate and Reservoir Modules

Plate directly on crushed ice does not reach 4°C in any of the wells and well-to-well temperature is uneven.

Plate placed on CoolSink module and then placed on ice shows more uniform well-to-well temperature and all wells at or below 4°C (Blue center plate wells are slightly warmer due to curvature of the underside of the plate.) Published in Biotechniques, November 2010.

CoolSink® XTSBS-compatible plate modules

CoolSink® LX55For 55 mL reagent reservoirs

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ThermalTray™ Thermo-Conductive Platforms

ThermalTray™ thermo-conductive platforms support CoolRack® and CoolSink® sample modules in liquid temperature sources such as melting ice, water baths and liquid nitrogen. Made of the same highly conductive alloy as CoolRack and CoolSink modules, ThermalTray platforms conduct the source temperature to the CoolRack or CoolSink and, ultimately, to your samples. The stable, sturdy design makes them ideal for processing temperature-sensitive samples in melted ice baths or liquid nitrogen. All modules may be autoclaved, high heat sterilized or decontaminated with bleach, alcohol or other disinfectants or lab detergents.

ThermalTray™ platforms are compatible with all temperature sources. If using in liquid nitrogen, the ThermalTray SLP or LP is highly recommended. These low profile ThermalTray platforms allow development of a vapor layer which will help insulate samples from ambient air.

ThermalTray™ Platforms

In IceKeep samples <4˚C for over 10 hours while ice melts

In Liquid Nitrogen Keep samples at -140˚C with liquid nitrogen

Item No. Description For Use With Dimensions (L x W x H)BCS-252 ThermalTray SLP, slim low-profile 9L ice pan with LN2 28.0 x 14.0 x 3.2 cmBCS-123 ThermalTray LP, low-profile 9L ice pan with crushed ice 28.0 x 14.0 x 5.1 cmBCS-104 ThermalTray HP, high-profile water bath 28.0 x 14.0 x 8.6 cm

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TruCool® Accessories

TruCool® Ice Pans and Buckets Non-toxic, recyclable ethyl-vinyl acetate (EVA) foam containers for use with ice, dry ice, liquid nitrogen, alcohol slurries. Will not sweat, leak or skid on bench.

Size Dimensions (L x W x H) ● purple ● lime green ● orange ● pink ● blue ● green ● red

Mini 1L Ice Pan 15.9 x 15.9 x 9.5 cm BCS-211PL BCS-211GR BCS-211OR BCS-211PK BCS-211B BCS-211 BCS-212

Midi 4L Ice Pan 31.1 x 22.2 x 11.4 cm BCS-113PL BCS-113GR BCS-113OR BCS-113PK BCS-113B BCS-113 BCS-114

Midi 4L Ice Pan with Lid

35.0 x 22.5 x 11.4cm BCS-117PL BCS-117GR BCS-117OR BCS-117PK BCS-117B

Maxi 9L Ice Pan 40.6 x 31.8 x 11.4 cm BCS-111PL BCS-111GR BCS-111OR BCS-111PK BCS-111B BCS-111 BCS-112

Maxi 9L Ice Pan with Lid

44.5 x 32.5 x 11.4 cm BCS-118PL BCS-118GR BCS-118B

Round 2.5L Ice Bucket with Lid

Top diameter: 24.1 cm, Height: 12.1 cm

BCS-115-25PL BCS-115-25GR BCS-115-25OR BCS-115-25PK BCS-115-25B BCS-115-25G BCS-115-25R

Round 4L Ice Bucket with Lid

Top diameter: 26.0 cm, Height: 18.4 cm

BCS-115PL BCS-115GR BCS-115OR BCS-115PK BCS-115B BCS-115 BCS-115R

TruCool® Hinged CryoBoxes Patented hinged lid offers convenience and archival integrity, ensuring markings and vials remain in sync. Lid stays attached to base minimizing risk of separation and lid contamination. Lid is easy to open when frozen. Available in 9x9,10x10, and vapor phase LN2 compatible formats. Plastic 81-place grid has adjustable slats to accommodate multiple vial types. 2-inch box holds 1.0 mL or 2.0 mL cryogenic vials and microfuge tubes. 3.5-inch box holds 3.0 mL to 5.0 mL cryogenic vials.

Freezer Storage CryoStorage2-inch Hinged CryoBox with adjustable plastic grid, 81-place

2-inch Hinged CryoBox with fixed plastic insert, 100-place

3.5-inch Hinged CryoBox with adjustable plastic grid, 81-place

2-inch Hinged CryoBox with adjustable plastic grid, LN2 drain holes, 81-place

Qty per pack 5 50 5 50 6 30 5 50

● white BCS-206 BCS-207

● blue BCS-206B BCS-207B

● green BCS-206G BCS-207G BCS-209G BCS-220G BCS-215G BCS-219G BCS-217G BCS-221G

● orange BCS-206O BCS-207O

● purple BCS-206P BCS-207P BCS-209P BCS-220P BCS-215P BCS-219P BCS-217P BCS-221P

● pink BCS-206PK BCS-207PK

multi-color (no white) BCS-206MC

Dimensions (L x W x H) 13.3 x 13.3 x 5.1 cm 5.25 x 5.25 x 2.0 in

13.3 x 13.3 x 5.1 cm 5.25 x 5.25 x 2.0 in

13.3 x 13.3 x 9.2 cm 5.25 x 5.25 x 3.5 in

13.3 x 13.3 x 5.1 cm 5.25 x 5.25 x 2.0 in

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Item No. DescriptionBCS-200 CoolProbe U, Temperature Monitor

TruCool® Cryogenic Vial Locking Racks Cryogenic vial locking racks feature a locking mechanism that allow one-hand opening for self-standing cryogenic vials. Accommodates both round bottom and self-standing vial formats. Racks have A - J and 1 - 5 row and column indexing for easy organization. Autoclavable. 5 per pack.

Item No. DescriptionBCS-222 TruCool Cryogenic Vial Locking Racks, multi-color

CryoCeps™ Cryogenic Vial Grippers Cryogenic vial grippers feature a unique design to grasp internal- or external-thread cryogenic vials. Easily sort or move vials while maintaining sterility and protecting fingers from frozen vials, dry ice and liquid nitrogen. 5 per pack.

Item No. DescriptionBCS-213MC CryoCeps Cryogenic Vial Grippers, multi-color

External Threads

Item No. BCS-2517 BCS-2501 BCS-2502 BCS-2503 BCS-2504 BCS-2505

Volume 1.0 mL 2.0 mL 2.0 mL 3.0 mL 4.0 mL 5.0 mL

Height* 40.1 mm 44.5 mm 45.5 mm 69.3 mm 73.9 mm 88.1 mm

Tube/Cap O.D. 12.5 mm 12.5 mm 12.5 mm 12.5 mm 12.5 mm 12.5 mm

Style Self-Standing Round-Bottom Self-Standing Self-Standing Self-Standing Self-Standing*Height with cap attached

TruCool® Barcoded Cryogenic Vials TruCool leak-proof, auto-cap cryogenic vials are ideal for cell culture and biobanking. The screw cap features a co-molded thermally-fused gasket which prevents leaking, slipping and risk of contamination. The gasket is 95kPa certified to provide a leak-proof seal. The star socket on cap top is compatible with auto-decapping equipment. Each vial is individually barcoded with a unique identifier that can be read with common barcode readers. Not recommended for storage directly in liquid nitrogen. Vapor phase ok. 500 per case.

Internal ThreadsItem No. BCS-2510 BCS-2511 BCS-2512 BCS-2513 BCS-2514 BCS-2515 BCS-2516

Volume 1.0 mL 2.0 mL 2.0 mL 4.0 mL 4.0 mL 5.0 mL 5.0 mL

Height* 42.7 mm 48.0 mm 47.0 mm 75.1 mm 76.1 mm 89.3 mm 90.4 mm

Tube/Cap O.D. 12.5 mm 12.5 mm 12.5 mm 12.5 mm 12.5 mm 12.5 mm 12.5 mm

Style Self-Standing Self-Standing Round-Bottom Round-Bottom Self-Standing Round-Bottom Self-Standing

Internal Threads External Threads

TruCool® Cryogenic Vial Cap InsertsInserts for auto caps. 1,000 per pack.

Color ● purple ● pink ● green ● yellow ● white ● red ● grey ● blue ● orange

Item No. BCS-2436 BCS-2432 BCS-2431 BCS-2434 BCS-2435 BCS-2433 BCS-2438 BCS-2430 BCS-2437

CoolProbe™ Temperature MonitorMeasure sample temperature with traceable temperature monitors. The CoolProbe U monitor is for use with sample tubes and plates. LCD digital display with minimum, maximum and current temperature display. Min/Max memory, high/low alarm. Certified NIST traceable.

Prep.

Standardization through the last mile.

Freeze.

Transport.

Store.

Thaw.

Ice-free sample cooling and freezing• Consistent and reproducible• Ideal for working in a hood

Controlled-rate cell freezing• No alcohol• High post-thaw recovery and viability• Proven for stem cells, primary cells,

PBMC, cell lines and more

Temperature stability for short transport• Ultra-low temperature portable and mobile systems• Safe, reliable and portable < -150°C cryogenic

environment

Archival storage integrity• Hinged lid helps box and lid stay together• LN2 drain holes and water proof fiberboard• -80°C freezer holds 36 2-inch cryoboxes

Automated cell thawing• Highly reproducible • High post-thaw recovery and viability• No fluids or contamination risk

© 2016. BioCision, LLC. All rights reserved. BioCision, Standardizing Samples, CoolCell, ThawSTAR, BioT, DIR, CoolRack, CoolSink, CoolBox, ThermalTray, TruCool, CryoCeps designations are trademarks owned by BioCision, LLC. CryoPod designation is a trademark owned by Brooks Automation. PN 20106 Rev 1

BioCision, LLC 101 Glacier Point Road, Suite E, San Rafael, CA 94901 USA [email protected] www.biocision.com

®

CoolRack® and CoolBox™ cooling systems

CoolCell® controlled-rate alcohol-free cell freezing containers

BioT™ temperature stability systems and CryoPod™ portable LN2 carrier

BioT™ ULT1.9 mini freezer and TruCool® hinged cryoboxes

ThawSTAR® CFT2 cell thawing system

Catalog 2016 - westburg.eu · ThawSTAR® CFT2 Instrument (green traces), a 37°C bead bath (Lab...

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Transcript of Catalog 2016 - westburg.eu · ThawSTAR® CFT2 Instrument (green traces), a 37°C bead bath (Lab...