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Romer LabsGuide to Mycotoxins

4th Edition

Rudolf Krska, John L. Richard,Rainer Schuhmacher, Andrew B. Slate,Thomas B. Whitaker

Edited by E.M. Binder & R. Krska

Levels of chemical contaminants in foods and feeds can originate from natural sources such as mycotoxins, which are toxic secondary metabolites produced by fungi on agricultural commodities in the field and during storage. More than 300 mycotoxins have been identified so far with widely different chemical structures and differing modes of action. The potential health risks to animals and humans posed by food- and feed-borne mycotoxin intoxication have been recognized by national and international organisations, which have addressed this

problem by adopting regulatory limits for major mycotoxin classes and selected individual mycotoxins. The Romer Labs Guide to Mycotoxins is a timely reflection of the research progress related to the significance, sampling and determination of mycotoxins. In this respect, the Romer Labs Guide will not only provide a thorough introduction into the history of mycotoxins and mycotoxicoses, but will also discuss the chemistry and toxic effects of the most common mycotoxins. The book will also provide guidance for the development of proper sampling and sample preparation techniques to obtain representative samples for the subsequent analysis of the food and feed commodities to be checked for mycotoxins. The analytical part of the Romer Labs Guide to Mycotoxins covers proper extraction and clean-up procedures, separation and detection techniques including latest state-of-the-art rapid strip tests. Special emphasis is put on multi-toxin methods based on liquid chromatography coupled with mass spectrometry for the simultaneous determination of a wide variety of secondary fungal metabolites which allow for a comprehensive assessment of the wide range of mycotoxins humans and animals are exposed to.

(115 Pages)

The "Romer Labs Guide to Mycotoxins" can be purchased on our website:>>shop.romerlabs.com>>

Content ........................................................... 3

Romer Labs® Worldwide ................................... 4

Romer Labs® History ......................................... 5

Mycotoxins ...................................................... 8

Aflatoxins ................................................... 9

Ochratoxins ................................................12

Trichothecenes ............................................13

Fumonisins .................................................15

Zearalenone ...............................................17

T-2/HT-2 Toxin ............................................19

Ergot Alkaloids ............................................21

Cleanup Columns ........................................22

Rapid Tests ................................................23

Reference Materials .....................................24

Enzymatic Food Analysis .................................30

Food Allergens .................................................32

Food Pathogens ..............................................34

Ruminant Meat & Bone Meal ............................36

GMO ................................................................38

Residues + Adulterants ...................................42

Veterinary Drug Residues .............................43

Other .......................................................44

Check Sample Survey .....................................45

Analytical Service ...........................................46

Equipment ......................................................50

Content

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San Antonio

Herzogenburg

Union, MO (USA) Newark, DE (USA)

Campinas (Brazil)

Singapore

Malaysia

Beijing (China)

Austria

Cheshire (UK)

Romer Labs® Subsidiary

Romer Labs® Distributor

Worldwide

Romer Labs Diagnostic GmbHTechnopark 1, A-3430 Tulln, Austria

Tel: +43 2272 615 3310Fax: +43 2272 615 33111

Email: [email protected]

Romer Labs Inc.1301 Stylemaster Drive

Union, MO 63084-1156, USATel: +1 636 583 8600Fax: +1 636 583 6553


Romer Labs do Brasil Imp. e Exp. Ltda.Estr. Municipal Campinas /

B. Campo Grande, s/n°, km 8.5Caixa Postal N° 1082,

CEP 13012-970 Campinas/SP, BrazilTel: +55 19 3261 1417Fax: +55 19 3261 1307


Romer Labs UK Ltd.Block 5

The Heath Business & Technical ParkRuncorn, Cheshire WA7 4QX

Tel: +44 845 519 50 10Email: [email protected]

Romer Labs Singapore Pte. Ltd.3791 Jalan Bukit Merah#08-08, e-Centre@RedhillSingapore 159471Tel: +65 6631 8018Fax: +65 6275 5584Email: [email protected]

Romer Labs (Beijing) Co. Ltd.1411-1416 Jia Tai International Mansion41 East 4th Ring Middle Road,Chaoyang DistrictBeijing 100025, ChinaTel: +86 10 8571 1914Fax: +86 10 8571 1944Email: [email protected]

Romer Labs Malaysia Sdn BhdSuite 218, 2nd FloorEureka ComplexUniversiti Sains Malaysia11800 Penang Malaysia Tel: +604 656 2851Fax: +604 656 2852Email: [email protected]

Romer Labs Technology, Inc.130, Sandy DriveNewark, DE 19713, U.S.A.Tel: +1 302 781 6400Fax: +1 302 781 6378Email: [email protected]

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Romer Labs®

Romer Labs® was founded in Washington,

MO, in 1982. Now, three decades later,

Romer Labs® has seven business units

(USA, Austria, Singapore, Brazil, China,

United Kingdom and Malaysia) on three

continents and is represented in more than

60 countries worldwide through dedicated

distributors.

Over the years, it has become the number-

one, niche provider of diagnostic solutions for

the agricultural, food and feed industry. Its

broad range of innovative tests and services

play a pivotal role in integrated food safety

solutions. Romer Labs® is at the forefront

of diagnostic technology and is constantly

expanding its product and service portfolio

to meet the continuously evolving demands

of its customers, supplying products and

services in the fields of mycotoxins, food

pathogens, food allergens, GMO and

veterinary drug residues.

The key objective at Romer Labs® is to provide

scientifically sound, high-quality products

and services, in line with its mission -

AgraQuant® ELISA Test

AgraStrip® Lateral Flow Devices

RapidChek®

Pathogen Detection

FluoroQuant® Fluorometric Test

MycoSep® and MultiSep® Cleanup Columns

StarLine™

Immunoaffinity Columns

Biopure® Standards, Calibrants and Reference Materials

Analytical Service

EnzymeFast®

Laboratory Equipment

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AgraQuant®

ELISA Tests

The Romer Labs® AgraQuant® product family is a range of enzyme-linked immunosorbent assay (ELISA) test kits that encompasses mycotoxin, allergen, GMO and drug residue tests. ELISA tests are quantitative tests. Reactions are read by a specific ELISA reader, such as the StatFax and the Chromate ELISA reader, for quantification purposes. A set of five to six standards used for calibration is supplied with all test kits to determine a result. Creating a new calibration curve with every assay setup assures high accuracy and reproducibility. The results obtained are comparable to HPLC results.AgraQuant® kits are direct competitive ELISAs for mycotoxin and drug residue tests and sandwich ELISAs for allergen and GMO tests. All AgraQuant® ELISAs use specific antibody coated microwells and an enzyme-labeled analyte conjugate. The quality of the antibody is crucial for test-result quality. Romer Labs® has a broad range of monoclonal antibodies, which are highly specific to the respective analyte and guarantee robust and accurate results.

AgraStrip® Lateral Flow Devices

The Romer Labs® AgraStrip® product family utilizes lateral flow technology, also known as immunochromatography tests. Most lateral flow devices (LFD) come in a format that is often called a dip stick or strip test. While the use of this technology is very widespread in the clinical field, the food safety market has only seen suitable applications in the last few years. AgraStrip® LFDs use specific antibodies labeled with colloidal gold for visual color development at a test and control zone. Developed LFDs can be read visually, making instrumentation redundant. They normally produce only a yes/no answer, indicating the presence or absence of an analyte at a defined concentration. However, if instruments such as the Romer Labs® AgraVision™ are used, LFDs can be read, providing a quantitative result. A digital camera measures the intensity of the reaction bands developing at the control and the test line in this method.

RapidChek® Pathogen Detection

As a food processor, choosing the best pathogen detection method can be a very difficult decision. The method should have a meaningful impact on the total cost of a food safety program but most importantly, provide maximum assurance of reliability in the test results. As a food safety professional, you know that it’s not just a test result. It is information that will help to protect

your customers and essentially your business.Romer Labs RapidChek® and RapidChek® SELECT™ product lines were developed to deliver the simplicity, speed and high accuracy necessary every day. Our technology sets us apart from other rapid test methods. The RapidChek® product family utilizes highly selective enrichment media coupled to advanced lateral flow immunochemical detection of food pathogens such as Salmonella spp., Listeria spp., and Escherichia coli O157 in various foodstuffs and environmental applications. Patented phage technology used in the SELECT™ system suppresses the growth of competitive microflora which creates optimal growth conditions for Salmonella spp. Highly specific monoclonal and polyclonal antibodies are exclusive to the RapidChek® system which works without the need for expensive equipment or extensive training. RapidChek®. Simply Accurate.

FluoroQuant® Fluorometric Test

The FluoroQuant® Test Kits are the fastest, quantitative fluorometric tests based on a solid-phase or immunoaffinity column cleanup. They are USDA/GIPSA approved and AOAC Performance Tested. These tests provide a wide quantitation range without dilution steps. The FluoroQuant® tests are simple enough for field use in grain, feed and food handling facilities, but they are also appropriate for analytical laboratories. Each kit comes complete with all test and accessory materials such as columns, filter paper, pipette tips, cuvettes and reagents.The FluoroQuant® Test kits require an FQ-Reader, which reads the fluorescence signal of the test and translates it into a quantitative analytical result. The FQ-Reader comes with a touch screen and can be connected to a PC and a printer.

MycoSep® and MultiSep® Cleanup Columns

In order to shorten the sample cleanup process, Romer Labs® has developed a product portfolio of one-step-cleanup columns for different groups of mycotoxins. MycoSep® and MultiSep® columns contain packing materials made of absorbent mixes designed for all relevant food and feed commodities. MycoSep® columns allow a one-step cleanup within 30 seconds: the MycoSep® cleanup column is pushed into a test tube containing the sample extract, forcing the extract to filter upwards through the packing material of the column. The interferences adhere to the chemical packing in the column and the purified extract, containing the analytes of interest, passes through the column. Similarly to the MycoSep® column format, the MultiSep® column format allows a one-step cleanup of sample extracts, containing the

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analyte of interest, within 30 seconds: the sample extract is applied on top of the MultiSep® cleanup column, allowing the extract to go through the packing material of the column by gravity, or alternatively, by forcing the extract through the column material via the application of vacuum or positive pressure. The interferences adhere to the chemical packing in the column and the purified extract, containing the analytes of interest, can be collected in a suitable vessel.

StarLine™ Immunoaffinity Columns

Romer Labs® wide range of immunoaffinity columns (IAC) for regulated mycotoxins is based on a specific antibody-analyte binding technology. StarLine™ immunoaffinity columns are designed for simple and reliable cleanups before the detection of mycotoxins in a broad range of foodstuffs and animal feed. Immunoaffinity columns contain a gel bed with toxin-specific antibodies coupled to the gel particles. These antibodies will capture mycotoxins in a sample and release them again after a washing stage. StarLine™ immunoaffinity columns have been validated in an extensive testing program and can be used for the purification and concentration of mycotoxins prior to the analysis by various techniques, such as HPLC, LC-MS, GC-MS, ELISA or direct fluorometry. All Romer Labs® IAC employ highly specific monoclonal antibodies.

Biopure Standards®, Calibrants and Reference Materials

Biopure is a Romer Labs brand which stands for high quality reference materials. All Biopure products are characterized by the appropriate independent methods, such as HPLC, NMR and MS. The selection of the right method is crucial to ensuring the identity and purity of the reference material. Biopure reference materials are accompanied by a certificate. The certificate states the uncertainty of the target analyte and documents the traceability of the certified value, expressed in SI conform units. The Certificate of Analysis is supplied in accordance to ISO Guide 31.Biopure has the broadest range of mycotoxin reference materials currently available globally. In addition to mycotoxins, Biopure supplies standards for veterinary drug residues, and other contaminants such as melamine.The stars of this product line are the fully labeled C13 internal standards, which are unique and rely on a patented technology, proprietary to Romer Labs. C13 standards play a crucial role in LC-MS/MS analysis and are widely used in multi-analyte methods.

Analytical Service

Romer Labs® currently operates service laboratories in four countries: Austria, Singapore, the United Kingdom and the United States. All these laboratories are accredited according to ISO 17025 and offer a broad range of services for allergens, mycotoxins, GMOs and veterinary drug residues. The Romer Labs® service laboratories employ cutting edged technology and are equipped with the latest generation of equipment.

Laboratory Equipment

Romer Labs® aims to provide full set-up packages for its diagnostic solutions. Using the right equipment is essential to performing a method correctly. Romer Labs® provides a series of readers for its rapid test kits, such as the AgraVision (for Lateral Flow Tests) or the Chromate (for ELISA tests). For instrumental analysis, Romer Labs® has different instruments available that help improve method performance, such as the RDU (Romer® Derivatization Unit) used for Aflatoxins analysis in HPLC-FLD. Romer® laboratory mills have set the standard in sample preparation in mycotoxin analysis for over 25 years and are referenced in numerous official methods (AOAC, USDA, FAO, etc.) and scientific publications. Many multinational food and feed companies use Romer® mills in their daily routine and the products are known for their reliability and robustness.

EnzymeFast®

EnzymeFast® tests are enzymatic tests in a micotiter plate format. Enzymatic food analysis is a basic method used for the measurement of compounds such as sugars, acids, alcohols and other metabolites in foods and beverages. In general, enzymes are proteins that catalyze the conversion of one molecule into another. Certain enzymatic reactions lead to color formation or consumption that often takes place in a wavelength range invisible to the human eye. This change in intensity can be measured. The high specificity of enzyme reactions allows for the analysis of food components in complex matrices. Enzymatic methods are especially important for food production and quality assurance. Results give information on the nutrients, authenticity and hygienic status of the food. For example, levels of sugars are crucial for consumers suffering from intolerances and the presence of certain metabolites indicates a lack of hygiene during production. It is also possible to detect whether food has been tampered with using enzymatic methods.

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Mycotoxins

Many species of fungi can produce a vast number of different mycotoxins. The presence of these toxins in food and feed can be of major concern in food and feed safety. Generally speaking, if elevated levels of mycotoxins are indicated, this can result in toxic, mutagenic and teratogenic effects and lead to symptoms such as skin irritation, immune suppression and neuro-toxicity, as well as to potential birth defects and death. The effects can be chronic or acute, depending on the concentration involved.

Mycotoxins are typically highly resistant to temperature and processing. Therefore, they cannot be destroyed during conventional food or feed production and can also be found in finished, processed products. Analytical procedures to find and identify mycotoxins are essential in ensuring proper risk management. Technologies and products are available for official field or on-site reference testing, depending on the intended use.

Romer Labs® offers a wide range of mycotoxin solutions, such as clean-up columns and reference materials for reference testing, as well as a complete portfolio of test kits for rapid testing.

FAO estimates global losses of foodstuffs due to mycotoxins, are in the range of 1000 million tonnes per year.”

“

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MycotoxinsAFLATOXINS

AgraQuant® ELISA Tests Item No. Quantitation Range Limit of Detection No. of Tests

AgraQuant® Total Aflatoxin COKAQ1100 1 – 20 ppb 1 ppb 96 COKAQ1148 1 – 20 ppb 1 ppb 48 COKAQ1000 4 – 40 ppb 3 ppb 96 COKAQ1048 4 – 40 ppb 3 ppb 48

AgraQuant® Aflatoxin B1 COKAQ8000 2 – 50 ppb 2 ppb 96 COKAQ8048 2 – 50 ppb 2 ppb 48

AgraQuant® Aflatoxin M1 COKAQ7100 25 – 500 ppt 18 ppt 96 COKAQ7200 100 – 200 ppt 89 ppt 96

AgraStrip® Lateral Flow Devices Item No. Quantitation Range Limit of Detection No. of Tests

AgraStrip® Afla COKAS11002 4 ppb 24 COKAS1100U2 incl. Whirl-Pak® bags 4 ppb 24 COKAS12001 10 ppb 24 COKAS1200U1 incl. Whirl-Pak® bags 10 ppb 24 COKAS10001 20 ppb 24 COKAS1000U1 20 ppb 24 COKAS1600A for AgraVision™ 5 – 100 ppb 2.6 ppb 24

AgraStrip® Aflatoxin M1 COKAS1500 100 – 600 ppt 47 ppt 24

FluoroQuant® Fluorometry Item No. Version Limit of Detection No. of Tests

FluoroQuant® Afla COKFA10101 – 3 ppb 25

FluoroQuant® Afla Plus COKFA30703 Acetonitrile from 0.5 ppb 25 COKFA3070D3 Methanol from 0.5 ppb 25

FluoroQuant® Afla IAC COKFA40101 US Domestic Version 3 ppb 25 COKFA4020 International Version 3 ppb 25

1USDA/GIPSA approved 2Japanese Department of Agriculture approved 3AOAC RI approved

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StarLine™ Immunoaffinity Columns Item No. Product Format No. of Columns

AflaStar™ COIAC1001 AflaStar™ FIT 1 mL 25 COIAC1501 AflaStar™ FIT 1 mL 500 COIAC1004 AflaStar™ R 3 mL 25 COIAC1504 AflaStar™ R 3 mL 500 COIAC1005 AflaStar™ M1 R 3 mL 25

MycoSep® & MultiSep® Cleanup Columns ItemNo. Product Specificity No.ofColumns

MycoSep®

COCMY2112 MycoSep® 112 AflaZon Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2224 MycoSep® 224 AflaZon 4 Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2226 MycoSep® 226 AflaZon+ Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2228 MycoSep® 228 AflaPat5 Aflatoxins B1, B2, G1 & G2, Patulin 25

MultiSep®

COCMU2224 MultiSep® 224 AflaZon Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMU2226 MultiSep® 226 AflaZon+ Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol

COCMU2228 MultiSep® 228 AflaPat5 Aflatoxins B1, B2, G1 & G2, Patulin 25

Biopure® Standards & Reference Materials Item No. Product Amount

Calibrant Solutions 002017 Aflatoxin B1 (AFB1), 2 µg/mL in acetonitrile 5 mL S02017 Aflatoxin B1 (AFB1), 2 µg/mL in acetonitrile 1 mL 002018 Aflatoxin B2 (AFB2), 0.5 µg/mL in acetonitrile 5 mL S02018 Aflatoxin B2 (AFB2), 0.5 µg/mL in acetonitrile 1 mL 002019 Aflatoxin G1 (AFG1), 2 µg/mL in acetonitrile 5 mL S02019 Aflatoxin G1 (AFG1), 2 µg/mL in acetonitrile 1 mL 002020 Aflatoxin G2 (AFG2), 0.5 µg/mL in acetonitrile 5 mL S02020 Aflatoxin G2 (AFG2), 0.5 µg/mL in acetonitrile 1 mL 002030 Aflatoxin M1 (AFM1), 0.5 µg/mL in acetonitrile 5 mL S02030 Aflatoxin M1 (AFM1), 0.5 µg/mL in acetonitrile 1 mL

4AOAC Offical Method 5 Japanese Official Method

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Calibrant Mixtures 002021 MIX 1 (Aflatoxins) 5 mL S02021 MIX 1 (Aflatoxins) 1 mL 002022 MIX 5 (Aflatoxins) 6 mL S02022 MIX 5 (Aflatoxins) 1 mL LMY-092-5mL MIX 9 (Aflatoxins) 5 mL

Stable Isotope Labeled Calibrant Mixtures* ILMO24-1.2 mL MIX 11 (13C Aflatoxins) 1.2 mL

Solid Standards

001012 Aflatoxin B1 (99.5 ± 0.5 purity) 5 mg 001013 Aflatoxin B2 (99.7 ± 0.3 purity) 5 mg 001014 Aflatoxin G1 (98.0 ± 2.0 purity) 5 mg 001015 Aflatoxin G2 (97.1 ± 2.9 purity) 5 mg

Stable Isotope Labeled Standards ILM010 U-[13C17]-Aflatoxin B1 (0.5 µg/mL) in acetonitrile 1.2 mL ILM-011-1.2ML U-[13C17]-Aflatoxin B2 (0.5 µg/mL) in acetonitrile 1.2 mL ILM-012-1.2ML U-[13C17]-Aflatoxin G1 (0.5 µg/mL) in acetonitrile 1.2 mL ILM-013-1.2ML U-[13C17]-Aflatoxin G2 (0.5 µg/mL) in acetonitrile 1.2 mL ILM-021-1.2ML U-[13C17]-Aflatoxin M1 (0.5 µg/mL) in acetonitrile 1.2 mL

Matrix Reference Materials†

003011 Aflatoxins in Maize 55 g AFB1: 7.4 ± 0.37 µg/kg; AFB2: 0.7 ± 0.07 µg/kg AFG1: < 0.1 µg/kg; AFG2: < 0.1 µg/kg Aflatoxins total: 8.9 ± 0.26 µg/kg

† These products represent naturally contaminated materials and have a limited supply. Subsequent products might have different characteristics.

* composition see page 27

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MycotoxinsOCHRATOXINS


AgraQuant® Ochratoxin COKAQ2000 2 – 40 ppb 1.9 ppb 96 COKAQ2048 2 – 40 ppb 1.9 ppb 48


OchraStar™ COIAC2001 OchraStar™ FIT 1 mL 25 COIAC2004 OchraStar™ R 3 mL 25


MycoSep®

COCMY2229 MycoSep® 229 Ochra Ochratoxin A 25

MultiSep®

COCMU2229 MultiSep® 229 Ochra Ochratoxin A 25


Calibrant Solutions 002023 Ochratoxin A (OTA) in acetonitrile 5 mL S02023 Ochratoxin A (OTA) in acetonitrile 1 mL S02052 Ochratoxin B (OTB) in acetonitrile 1 mL S02053 Ochratoxin-alpha (OTα) in acetonitrile 1 mL

Solid Standards 001008 Ochratoxin A (99.5 ± 0.5 purity) 5 mg 001108 Ochratoxin A (99.5 ± 0.5 purity) 10 mg

Stable Isotope Labeled Standards ILM007 U-[13C20]-Ochratoxin A (10 µg/mL) in acetonitrile 1.2 mL

Check Sample Materials†

003023 Ochratoxin A in wheat 100 g (8.6 ± 3.6 µg/kg) † These products represent naturally contaminated materials and have a limited supply. Subsequent products might

have different characteristics.

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MycotoxinsTRICHOTHECENES

AgraQuant® ELISA Tests Item No Quantitation Range Limit of Detection No. of Tests

AgraQuant® Deoxynivalenol COKAQ4000 250 – 5000 ppb 1,2,3 200 ppb 96 COKAQ4048 250 – 5000 ppb 1,2,3 200 ppb 48


AgraStrip® DON Quantitative COKAS4000A 250 – 5000 ppb 140 ppb 24 for AgraVision™


DONStar™ COIAC5004 DONStar™ R 3 mL 25 COIAC5504 DONStar™ R 3 mL 500


MycoSep®

COCMY2113 MycoSep® 113 Trich Trichothecenes, Type A & B 25 COCMY2225 MycoSep® 225 Trich 4, 6 Trichothecenes, Type A & B 25 COCMY2227 MycoSep® 227 Trich+ 5 Trichothecenes, Type A & B 25

MultiSep®

COCMU2216 MultiSep® 216 Trichothecenes, Type A & B 25 COCMU2225 MultiSep® 225 Trich Trichothecenes, Type A & B 25 COCMU2227 MultiSep® 227 Trich+ 5 Trichothecenes, Type A & B 25

1USDA/GIPSA approved2 Japanese Department of Agriculture approved

3AOAC RI approved4AOAC offical method

5Japanese official method6 Chinese Quarantine Inspection method

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MycotoxinsTRICHOTHECENES


Calibrant Solutions 002014 15-Acetyl-Deoxynivalenol (15-AcDON) in acetonitrile 5 mL S02014 15-Acetyl-Deoxynivalenol (15-AcDON) in acetonitrile 1 mL 002012 3-Acetyl-Deoxynivalenol (3-AcDON) in acetonitrile 5 mL S02012 3-Acetyl-Deoxynivalenol (3-AcDON) in acetonitrile 1 mL 002033 Deepoxy-deoxynivalenol in acetonitrile 5 mL S02033 Deepoxy-deoxynivalenol in acetonitrile 1 mL 002009 Deoxynivalenol (DON) in acetonitrile 5 mL S02009 Deoxynivalenol (DON) in acetonitrile 1 mL S02046 Deoxynivalenol-3-glucoside in acetonitrile 1 mL

Calibrant Mixtures* 002002 MIX 4 - 3-AcDON,DON,NIV,FusX,HT-2,T-2,DAS,ZON 5 mL S02002 MIX 4 - 3-AcDON,DON,NIV,FusX,HT-2,T-2,DAS,ZON 1 mL LMY-093-5mL MIX 8 - DON, HT-2, T-2, ZON 5 mL

Stable Isotope Labeled Calibrant Mixtures* ILM-025-1.2mL MIX 10 - 13C15DON, 13C22HT-2, 13C24T-2, 13C18ZON 1.2 mL

Solid Standards 001006 15-Acetyldeoxynivalenol (98.8 ± 1.2 purity) 5 mg 001106 15-Acetyldeoxynivalenol (98.8 ± 1.2 purity) 10 mg 001003 3-Acetyldeoxynivalenol (99.4 ± 0.6 purity) 5 mg 001103 3-Acetyldeoxynivalenol (99.4 ± 0.6 purity) 10 mg 001001 Deoxynivalenol (99.4 ± 0.6 purity) 5 mg 001101 Deoxynivalenol (99.4 ± 0.6 purity) 10 mg

Stable Isotope Labeled Standards ILM006 U-[13C17]-3-Acetyl-Deoxynivalenol (25 µg/mL) in acetonitrile 1.2 mL 002005 U-[13C17]-Deoxynivalenol (25 µg/mL) in acetonitrile 1.2 mL


003022 Deoxynivalenol in Wheat (877 ± 23 µg/kg) 55 g 003014 Deoxynivalenol in Maize (1840 ± 30 µg/kg) 55 g


003012 Deoxynivalenol in Maize (2110 ± 290 µg/kg) 003024 Zearalenone and Deoxynivalenol in Maize ZON (79 ± 26 µg/kg), DON: 901 ± 110 µg/kg) 003025 Deoxynivalenol in wheat DON: 143 ± 256; Sum of 3AcDON and 15 AcDON < 50 100 g


*composition see page 27

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MycotoxinsFUMONISINS


AgraQuant® Fumonisin COKAQ30001 250 5000 ppb 200 ppb 96 COKAQ30481 250 – 5000 ppb 200 ppb 48


AgraStrip® Fumonisin Quantitative COKAS3000A for AgraVision™ 500 – 5000 ppb 300 ppb 24


FumoniStar™ COIAC3000 FumoniStar™ 3 mL 25


MycoSep®

COCMY2231 MycoSep® 231 FUM Fumonisin B1, B2 & B3 25

MultiSep®

COCMU2211 MultiSep® 211 FUM Fumonisin B1, B2 & B3 25


Calibrant Solutions 002003 Fumonisin B1 (50 µg/mL) in acetonitrile/water 5 mL S02003 Fumonisin B1 (50 µg/mL) in acetonitrile/water 1 mL 002004 Fumonisin B2 (50 µg/mL) in acetonitrile/water 5 mL S02004 Fumonisin B2 (50 µg/mL) in acetonitrile/water 1 mL S02007 Fumonisin B3 (50 µg/mL) in acetonitrile/water 1 mL LMY-087-1ML Hydrolyzed Fumonisin B1 (25 µg/mL) in acetonitrile/water 1 mL on request

Calibrant Mixtures* 002006 MIX 3 - Fumonisin B1 & B2 (50 µg/mL each) in AcN/water 5 mL S02006 MIX 3 - Fumonisin B1 & B2 (50 µg/mL each) in AcN/water 1 mL

Stable Isotope Labeled Calibrant Mixtures* ILM-023-1.2ML MIX 12 - 13C34 Fumonisin B1 & B2 (50 µg/mL each) in AcN/water 1.2 mL

1USDA/GIPSA approved *composition see page 27

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MycotoxinsFUMONISINS


Solid Standards 001007 Fumonisin B1 (97.6 ± 2.4 purity) 5 mg 001107 Fumonisin B1 (97.6 ± 2.4 purity) 10 mg

Stable Isotope Labeled Standards ILM003 U-[13C34]-Fumonisin B1 (25 µg/mL) in acetonitrile/water 1.2 mL ILM004 U-[13C34]-Fumonisin B2 (10 µg/mL) in acetonitrile/water 1.2 mL ILM005 U-[13C34]-Fumonisin B3 (10 µg/mL) in acetonitrile/water 1.2 mL


003017 Fumonisins in Maize 100g FB1: 2630 ± 740 µg/kg; FB2: 690 ± 340 µg/kg; FB3: 310 ± 210 µg/kg 003018 Fumonisins in Maize 100 g FB1: 270 ± 110 µg/kg; FB2: < 80 µg/kg; FB3: < 80 µg/kg


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MycotoxinsZEARALENONE


AgraQuant® Zearalenone COKAQ5000 25 – 1000 ppb 20 ppb 96 COKAQ5048 25 – 1000 ppb 20 ppb 48


ZearaStar™ COIAC4004 ZearaStar™ R 3 mL 25


MycoSep®

COCMY2112 MycoSep® 112 AflaZon Aflatoxins B1, B2, G1 & G2, Zearalenone, Zearalenol, Zearalanol 25 COCMY2224 MycoSep® 224 AflaZon Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2226 MycoSep® 226 AflaZon+ Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol

MultiSep®

COCMU2224 MultiSep® 224 AflaZon Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMU2226 MultiSep® 226 AflaZon+ Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol


Calibrant Solutions 002029 Zearalenone (ZON) (100 µg/mL) in acetonitrile 5 mL S02029 Zearalenone (ZON) (100 µg/mL) in acetonitrile 1 mL S02041 Zearalanone (ZAN) (10 µg/mL) in acetonitrile 1 mL on request

Calibrant Mixtures* 002002 MIX 4 - 3-AcDON, DON, NIV, FusX, 5 mL HT-2, T-2, DAS, ZON S02002 MIX 4 - 3-AcDON, DON, NIV, FusX, 1 mL HT-2, T-2, DAS, ZON LMY-093-5ML MIX 8 - DON, HT-2, T-2, ZON 5 mL

Stable Isotope Labeled Calibrant Mixtures* ILM-025-1.2 ML MIX 10 - 13C15 DON, 13C22 HT-2, 13C24 T-2, 13C18 ZON 1.2 mL


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MycotoxinsZEARALENONE


Solid Standards 001009 Zearalenone (99.4 ± 0.6 purity) 5 mg 001109 Zearalenone (99.4 ± 0.6 purity) 10 mg

Stable Isotope Labeled Standards ILM009 U-[13C18]-Zearalenone (25 µg/mL) in acetonitrile 1.2 mL


003024 Zearalenone and Deoxynivalenol in Maize 100 g ZON: 79 ± 26 µg/kg, DON: 901 ± 110 µg/kg


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MycotoxinsT-2/HT-2 TOXIN


AgraQuant® T-2 Toxin COKAQ6000 25 – 500 ppb 20 ppb 96 COKAQ6048 25 – 500 ppb 20 ppb 48

AgraQuant® T-2/HT-2 Toxin COKAQ6100 37 – 500 ppb 29 ppb 96


T2/HT2 Star™ COIAC6004 T2/HT2Star™ R 3 mL 25


MycoSep®

COCMY2113 MycoSep® 113 Trich Trichothecenes, Type A & B 25 COCMY2225 MycoSep® 225 Trich 4, 6 Trichothecenes, Type A & B 25 COCMY2227 MycoSep® 227 Trich+ 5 Trichothecenes, Type A & B 25

MultiSep®

COCMU2216 MultiSep® 216 Trich Trichothecenes, Type A & B 25 COCMU2225 MultiSep® 225 Trich Trichothecenes, Type A & B 25 COCMU2227 MultiSep® 227 Trich+ 5 Trichothecenes, Type A & B 25

4AOAC Offical Method 5Japanese Official Method6 Chinese Quarantine Inspection method

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MycotoxinsT-2/HT-2 TOXIN


Calibrant Solutions 002036 HT-2 Toxin (100 µg/mL) in acetonitrile 5 mL S02036 HT-2 Toxin (100 µg/mL) in acetonitrile 1 mL 002035 T-2 Toxin (100 µg/mL) in acetonitrile 5 mL S02035 T-2 Toxin (100 µg/mL) in acetonitrile 1 mL S02048 T-2 Tetraol (50 µg/mL) in acetonitrile 1 mL S02037 T-2 Triol (50 µg/mL) in acetonitrile 1 mL

Calibrant Mixtures* 002002 MIX 4 - 3-AcDON, DON, NIV, FusX, 5 mL HT-2, T-2, DAS, ZON S02002 MIX 4 - 3-AcDON, DON, NIV, FusX, 1 mL HT-2, T-2, DAS, ZON LMY-093-5mL MIX 8 - DON, HT-2, T-2, ZON 5 mL

Stable Isotope Labeled Calibrant Mixtures* ILM-025-1.2mL MIX10 - 13C15 DON, 13C22 HT-2, 13C24 T-2, 13C18 ZON 1.2 mL

Solid Standards 001011 T-2 Toxin (99.7 ± 0.3 purity) 5 mg 001111 T-2 Toxin (99.7 ± 0.3 purity) 10 mg

Stable Isotope Labeled Standards

ILM0008 U-[13C22]-HT-2 Toxin (25 µg/mL) in acetonitrile 1.2 mL 002044 U-[13C24]-T-2 Toxin (25 µg/mL) in acetonitrile 1.2 mL


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MycotoxinsERGOT ALKALOIDS


MycoSep®

COCMY2150 MycoSep® 150 Ergots Ergot Alkaloids 25


Calibrant Solutions 002064 Ergocornine (100 µg/mL), dried down 5 mL 002072 Ergocorninine (25 µg/mL), dried down 5 mL 002065 Ergocristine (100 µg/mL), dried down 5 mL 002073 Ergocristinine (25 µg/mL), dried down 5 mL 002066 Ergocryptine (100 µg/mL), dried down 5 mL 002074 Ergocryptinine (25 µg/mL), dried down 5 mL 002067 Ergometrine (100 µg/mL), dried down 5 mL LMY-090-5ML Ergometrinine (25 µg/mL), dried down 5 mL 002068 Ergosine (100 µg/mL), dried down 5 mL LMY-089-5ML Ergosinine (25 µg/mL), dried down 5 mL 002069 Ergotamine (100 µg/mL), dried down 5 mL 002075 Ergotaminine (25 µg/mL), dried down 5 mL LMY-085-5mL Dihydroergocristine (100 µg/mL), dried down 5 mL on request

Calibrant Mixtures* 002070 MIX 6 - Ergot Alkaloids 5 mL Ergocornine, Ergocristine, Ergocryptine, Ergometrine, Ergosine, Ergotamine; 100 µg/mL each, dried down


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MycotoxinsCLEANUP COLUMNS

StarLine™ Immunoaffinity Columns ItemNo. Product Specificity Format No.ofColumns

COIAC1001 AflaStar™ FIT Aflatoxins B1, B2, G1 & G2 1 mL 25 COIAC1004 AflaStar™ R Aflatoxins B1, B2, G1 & G2 3 mL 25 COIAC1005 AflaStar™ M1 R Aflatoxin M1 3 mL 25 COIAC1501 AflaStar™ FIT Aflatoxins B1, B2, G1 & G2 1 mL 500 COIAC1504 AflaStar™ R Aflatoxins B1, B2, G1 & G2 3 mL 500 COIAC2001 OchraStar™ FIT Ochratoxin A 1 mL 25 COIAC2004 OchraStar™ R Ochratoxin A 3 mL 25 COIAC3000 FumoniStar™ Fumonisin B1, B2, B3 3 mL 25 COIAC4004 ZearaStar™ R Zearalenone 3 mL 25 COIAC5004 DONStar™ R Deoxynivalenol 3 mL 25 COIAC5504 DONStar™ R Deoxynivalenol 3 mL 500 COIAC6004 T2/HT2 Star™ R T2 Toxin HT2 Toxin 3 mL 25


MycoSep®

COCMY2112 MycoSep® 112 AflaZon Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2113 MycoSep® 113 Trich Trichothecenes, Type A & B 25 COCMY2150 MycoSep® 150 Ergot Ergot Alkaloids 25 COCMY2224 MycoSep® 224 AflaZon4 Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2225 MycoSep® 225 Trich 4, 6 Trichothecenes, Type A & B 25 COCMY2226 MycoSep® 226 AflaZon+ Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMY2227 MycoSep® 227 Trich+5 Trichothecenes, Type A & B 25 COCMY2228 MycoSep® 228 AflaPat5 Aflatoxins B1, B2, G1 & G2, Patulin 25 COCMY2229 MycoSep® 229 Ochra Ochratoxin A 25 COCMY2230 MycoSep® 230 Niv Nivalenol 25 COCMY2231 MycoSep® 231 Fum Fumonisin B1, B2 & B3 25 COCMY2240 MycoSep® 240 Mon Moniliformin 25

MultiSep®

COCMU2211 MultiSep® 211 Fum Fumonisin B1, B2 & B3 25 COCMU2216 MultiSep® 216 Trichothecenes, Type A & B 25 COCMU2224 MultiSep® 224 AflaZon Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMU2225 MultiSep® 225 Trich Trichothecenes, Type A & B 25 COCMU2226 MultiSep® 226 AflaZon+ Aflatoxins B1, B2, G1 & G2, 25 Zearalenone, Zearalenol, Zearalanol COCMU2227 MultiSep® 227 Trich+5 Trichothecenes, Type A & B 25 COCMU2228 MultiSep® 228 AflaPat5 Aflatoxins B1, B2, G1 & G2, Patulin 25 COCMU2229 MultiSep® 229 Ochra Ochratoxin A 25 COCMU2230 MultiSep® 230 Niv Nivalenol 25

MycoSpin™ Column ItemNo. Product Specificity No.ofColumns

COCMY2400 MycoSpin™ 400 Multitoxin Aflatoxin B1, B2, G1 & G2, Trichothecenes Type A & B, Zearalenone, Ochratoxin A 25

4AOAC official method 5Japanese official method 6 Chinese Quarantine Inspection method

NEW

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MycotoxinsRAPID TESTS

AgraQuant® ELISA Tests Item No. Product Quantitation Range LOD No. of Tests

COKAQ1000 AgraQuant® Aflatoxin 4 – 40 ppb 3 ppb 96 COKAQ1048 AgraQuant® Aflatoxin 4 – 40 ppb 3 ppb 48 COKAQ1100 AgraQuant® Aflatoxin2 1 – 20 ppb 1 ppb 96 COKAQ1148 AgraQuant® Aflatoxin2 1 – 20 ppb 1 ppb 48 COKAQ7100 AgraQuant® Aflatoxin M1 25 – 500 ppt 0.018 ppb 96 COKAQ7200 AgraQuant® Aflatoxin M1 100 – 2000 ppt 0.089 ppb 96 COKAQ8000 AgraQuant® Aflatoxin B1 2 – 50 ppb 2 ppb 96 COKAQ8048 AgraQuant® Aflatoxin B1 2 – 50 ppb 2 ppb 48 COKAQ2000 AgraQuant® Ochratoxin 2 – 40 ppb 1.9 ppb 96 COKAQ2048 AgraQuant® Ochratoxin 2 – 40 ppb 1.9 ppb 48 COKAQ3000 AgraQuant® Fumonisin1 250 – 5000 ppb 200 ppb 96 COKAQ3048 AgraQuant® Fumonisin1 250 – 5000 ppb 200 ppb 48 COKAQ4000 AgraQuant® Deoxynivalenol 250 – 5000 ppb 200 ppb 96 COKAQ4048 AgraQuant® Deoxynivalenol 250 – 5000 ppb 200 ppb 48 COKAQ5000 AgraQuant® Zearalenone 25 – 1000 ppb 20 ppb 96 COKAQ5048 AgraQuant® Zearalenone 25 – 1000 ppb 20 ppb 48 COKAQ6000 AgraQuant® T-2 Toxin 25 – 500 ppb 20 ppb 96 COKAQ6048 AgraQuant® T-2 Toxin 25 – 500 ppb 20 ppb 48 COKAQ6100 AgraQuant® T-2/HT-2 Toxin 37 – 500 ppb 29 ppb 96

AgraStrip® Lateral Flow Devices Item No. Product Quantitation Range LOD No. of Tests

COKAS1100 AgraStrip® Aflatoxin2 cut-off 4 ppb - 24 COKAS1100U AgraStrip® Aflatoxin2 cut-off 4 ppb - 24 COKAS1200 AgraStrip® Aflatoxin1 cut-off 10 ppb - 24 COKAS1200U AgraStrip® Aflatoxin1 cut-off 10 ppb - 24 COKAS1000 AgraStrip® Aflatoxin1 cut-off 20 ppb - 24 COKAS1000U AgraStrip® Aflatoxin1 cut-off 20 ppb - 24 COKAS1500 AgraStrip® Aflatoxin M1 100 – 600 ppt 47 ppt 24 COKAS1600A AgraStrip® Aflatoxin 5 – 100 ppb 3.6 ppb 24 for AgraVision™ Reader COKAS3000A AgraStrip® DON 500 – 5000 ppb 300 ppb 24 for AgraVision™ Reader COKAS4000A AgraStrip® FUM 250 – 5000 ppb 190 ppb 24 for AgraVision™ Reader

FluoroQuant® Fluorometry Item No. Product Version LOD No. of Tests

COKFA1010 FluoroQuant® Afla1 - 3 ppb 25 COKFA3070 FluoroQuant® Afla Plus3 acetonitrile from 0.5 ppb 25 COKFA3070D FluoroQuant® Afla Plus3 methanol from 0.6 ppb 25 COKFA4010 FluoroQuant® Afla IAC1 US Domestic Version 3 ppb 25 COKFA4020 FluoroQuant® Afla IAC International Version 3 ppb 25

1USDA/GIPSA approved 2Japanese Department of Agriculture approved

3AOAC RI approved

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MycotoxinsREFERENCE MATERIALS


Calibrant Solutions LMY-088-1ML 15-Acetoxyscirpenol (50 µg/mL) in acetonitrile 1 mL on request 002014 15-Acetyl-Deoxynivalenol (15-AcDON) (100 µg/mL) in AcN 5 mL S02014 15-Acetyl-Deoxynivalenol (15-AcDON) (100 µg/mL) in AcN 1 mL 002012 3-Acetyl-Deoxynivalenol (3-AcDON) (100 µg/mL) in acetonitrile 5 mL S02012 3-Acetyl-Deoxynivalenol (3-AcDON) (100 µg/mL) in acetonitrile 1 mL 002017 Aflatoxin B1 (AFB1) (2 µg/mL) in acetonitrile 5 mL S02017 Aflatoxin B1 (AFB1) (2 µg/mL) in acetonitrile 1 mL 002018 Aflatoxin B2 (AFB2) (0.5 µg/mL) in acetonitrile 5 mL S02018 Aflatoxin B2 (AFB2) (0.5 µg/mL) in acetonitrile 1 mL 002019 Aflatoxin G1 (AFG1) (2 µg/mL) in acetonitrile 5 mL S02019 Aflatoxin G1 (AFG1) (2 µg/mL) in acetonitrile 1 mL 002020 Aflatoxin G2 (AFG2) (0.5 µg/mL) in acetonitrile 5 mL S02020 Aflatoxin G2 (AFG2) (0.5 µg/mL) in acetonitrile 1 mL 002030 Aflatoxin M1 (0.5 µg/mL) in acetonitrile 5 mL S02030 Aflatoxin M1 (0.5 µg/mL) in acetonitrile 1 mL 002071 Agroclavine (100 µg/mL) in acetonitrile 5 mL S02054 Alpha-Zearalanol (Zeranol) (10 µg/mL) in acetonitrile 1 mL S02042 Alpha-Zearalenol (10 µg/mL) in acetonitrile 1 mL S02076 Alternariol (100 µg/mL) in acetonitrile 1 mL S02077 Alternariolmethylether (100 µg/mL) in acetonitrile 1 mL LMY-056-1ML Beauvericin (100 µg/mL) dried down 1 mL on request S02055 Beta-Zearalanol (Taleranol) (10 µg/mL) in acetonitrile 1 mL S02043 Beta-Zearalenol (10 µg/mL) in acetonitrile 1 mL LMY-084-1ML Citreoviridin (100 µg/mL) dried down 1 mL on request S02063 Citrinin (100 µg/mL) in acetonitrile 1 mL on request LMY-095-1mL Cyclopiazonic Acid (100 µg/mL) in acetonitrile 1 mL on request 002033 Deepoxy-deoxynivalenol (50 µg/mL) in acetonitrile 5 mL S02033 Deepoxy-deoxynivalenol (50 µg/mL) in acetonitrile 1 mL 002009 Deoxynivalenol (DON) (100 µg/mL) in acetonitrile 5 mL S02009 Deoxynivalenol (DON) (100 µg/mL) in acetonitrile 1 mL S02046 Deoxynivalenol-3-glucoside (50 µg/mL) in acetonitrile 1 mL 002037 Diacetoxyscirpenol (DAS) (100 µg/mL) in acetonitrile 5 mL S02037 Diacetoxyscirpenol (DAS) (100 µg/mL) in acetonitrile 1 mL S02056 Fumagillin (100 µg/mL) in acetonitrile 1 mL 002003 Fumonisin B1 (FB1) (50 µg/mL) in acetonitrile/water 5 mL S02003 Fumonisin B1 (FB1) (50 µg/mL) in acetonitrile/water 1 mL LMY-087-1ML Fumonisin B1, hydrolyzed (HFB1) (25 µg/mL) in /water 1 mL on request 002004 Fumonisin B2 (FB2) (50 µg/mL) in acetonitrile/water 5 mL S02004 Fumonisin B2 (FB2) (50 µg/mL) in acetonitrile/water 1 mL S02007 Fumonisin B3 (FB3) (50 µg/mL) in acetonitrile/water 1 mL 002010 Fusarenon X (FusX) (100 µg/mL) in acetonitrile 5 mL S02010 Fusarenon X (FusX) (100 µg/mL) in acetonitrile 1 mL S02057 Gliotoxin (100 µg/mL) in acetonitrile 1 mL on request

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Calibrant Solutions 002036 HT-2 Toxin (HT-2) (100 µg/mL) in acetonitrile 5 mL S02036 HT-2 Toxin (HT-2) (100 µg/mL) in acetonitrile 1 mL LMY-083-1ML Kojic acid (100 µg/mL) in dried down 1 mL on request LMY-081-1ML Meleagrin (100 µg/mL) in dried down 1 mL on request S02058 Moniliformin (MON) (100 µg/mL) in acetonitrile/water 1 mL 002049 Mycophenolic acid (100 µg/mL) in acetonitrile 5 mL on request S02049 Mycophenolic acid (100 µg/mL) in acetonitrile 1 mL on request 002001 Neosolaniol (NEO) (100 µg/mL) in acetonitrile 5 mL S02001 Neosolaniol (NEO) (100 µg/mL) in acetonitrile 1 mL 002011 Nivalenol (NIV) (100 µg/mL) in acetonitrile 5 mL S02011 Nivalenol (NIV) (100 µg/mL) in acetonitrile 1 mL 002023 Ochratoxin A (OTA) (10 µg/mL) in acetonitrile 5 mL S02023 Ochratoxin A (OTA) (10 µg/mL) in acetonitrile 1 mL S02052 Ochratoxin B (OTB) (10 µg/mL) in acetonitrile 1 mL S02053 Ochratoxin-alpha (OTα) (10 µg/mL) in acetonitrile 1 mL 002026 Patulin (PAT) (100 µg/mL) in acetonitrile 5 mL S02026 Patulin (PAT) (100 µg/mL) in acetonitrile 1 mL S02059 Paxilline (100 µg/mL) in acetonitrile 1 mL on request S02060 Penicillic acid (100 µg/mL) acetonitrile 1 mL S02079 Roquefortine C (100 µg/mL) dried down 1 mL LMY-091-1.2ML Secalonic Acid D (50 µg/mL) in chloroform 1.2 mL LMY-086-1ML Stachybotrylactam (100 µg/mL) dried down 1 mL on request 002051 Sterigmatocystin (50 µg/mL) in acetonitrile 5 mL on request S02051 Sterigmatocystin (50 µg/mL) in acetonitrile 1 mL on request S02048 T-2 Tetraol (50 µg/mL) in acetonitrile 1 mL 002035 T-2 Toxin (T-2) (100 µg/mL) in acetonitrile 5 mL S02035 T-2 Toxin (T-2) (100 µg/mL) in acetonitrile 1 mL S02047 T-2 Triol (50 µg/mL) in acetonitrile 1 mL S02078 Tentoxin (100 µg/mL) dried down 1 mL LMY-080-1ML Tenuazonic acid (100 µg/mL) dried down 1 mL on request S02061 Verruculogen (100 µg/mL) in acetonitrile 1 mL S02062 Wortmannin (100 µg/mL) in acetonitrile 1 mL on request S02041 Zearalanone (ZAN) (10 µg/mL) in acetonitrile 1 mL on request 002029 Zearalenone (ZON) (100 µg/mL) in acetonitrile 5 mL S02029 Zearalenone (ZON) (100 µg/mL) in acetonitrile 1 mL

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Ergot Alkaloids 002064 Ergocornine (100 µg/kg) dried down 5 mL 002072 Ergocorninine (25 µg/kg) dried down 5 mL 002065 Ergocristine (100 µg/kg) dried down 5 mL 002073 Ergocristinine (25 µg/kg) dried down 5 mL 002066 Ergocryptine (100 µg/kg) dried down 5 mL 002074 Ergocryptinine (25 µg/kg) dried down 5 mL 002067 Ergometrine (100 µg/kg) dried down 5 mL LMY-090-5ML Ergometrinine (25 µg/kg) dried down 5 mL 002068 Ergosine (100 µg/kg) dried down 5 mL LMY-089-5ML Ergosinine (25 µg/kg) dried down 5 mL 002069 Ergotamine (100 µg/kg) dried down 5 mL 002075 Ergotaminine (25 µg/kg) dried down 5 mL LMY-085-5mL Dihydroergocristine 5 mL on request

Calibrant Mixtures*

002021 MIX 1 (Aflatoxins) 5 mL S02021 MIX 1 (Aflatoxins) 1 mL 002026 MIX 2 (B Trichothecenes) 5 mL S02026 MIX 2 (B Trichothecenes) 1 mL 002006 MIX 3 (Fumonisins) 5 mL S02006 MIX 3 (Fumonisins) 1 mL 002002 MIX 4 (A & B Trichothecenes, ZON) 5 mL S02002 MIX 4 (A & B Trichothecenes, ZON) 1 mL 002022 MIX 5 (Aflatoxins) 5 mL S02022 MIX 5 (Aflatoxins) 1 mL 002070 MIX 6 (Ergot Alkaloids) 5 mL LMY-093-5mL MIX 8 (Fusarium toxins) 5 mL LMY-092-5mL MIX 9 (Aflatoxins) 5 mL

Stable Isotope Labeled Calibrant Mixtures*

ILM-025-1.2mL MIX 10 (13C Fusarium toxins) 1.2 mL ILM-024-1.2mL MIX 11 (13C Aflatoxins) 1.2 mL ILM-023-1.2mL MIX 12 (13C Fumonisins) 1.2 mL

* composition see page 27

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Biopure® Standards & Reference MaterialsComposition of Mycotoxin Calibrant Mixtures

MIX 1 2 µg/mL Aflatoxin B1, 2 µg/mL Aflatoxin G1, 0.5 µg/mL Aflatoxin B2, 0.5 µg/mL Aflatoxin G2 in acetonitrile

MIX 2 100 µg/mL each of Deoxynivalenol, Nivalenol, 3-Acetyl-deoxynivalenol, 15-Acetyl-deoxynivalenol in acetonitrile

MIX 3 50 µg/mL each Fumonisin B1, Fumonisin B2 in acetonitile/water

MIX 4 10 µg/mL each 3-Acetyl-doxynivalenol, Deoxynivalenol, Nivalenol, Fusarenon X, HT-2 Toxin, T-2 Toxin, Diacetoxyscirperiol, Zearalenone in acetonitrile

MIX 5 0.25 µg/mL each Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2 in acetonitrile

MIX 6 100 µg/mL each Ergocornine, Ergocristine, Ergocryptine, Ergometrine, Ergosine, Ergotamine dried down

MIX 8 100 µg/mL Deoxynivalenol, 100 µg/mL HT-2Toxin, 10 µg/mL T-2 Toxin, 30 µg/mL Zearalenone MIX 9 1 µg/mL Aflatoxin B1, 1 µg/mL Aflatoxin B2, 1 µg/mL Aflatoxin G1, 1 µg/mL Aflatoxin G2 in acetonitrile

MIX 10 10 µg/mL 13C15 Deoxynivalenol, 10 µg/mL 13C22HT-2Toxin, 1 µg/mL 13C24 T-2Toxin, 3 µg/mL 13C18 Zearalenone in acetonitrile MIX 11 0.5 µg/mL 13C17 Aflatoxin B1, 0.5 µg/mL 13C17 Aflatoxin B2, 0.5 µg/mL 13C17 Aflatoxin G1, 0.5 µg/mL 13C17 Aflatoxin G2 in acetonitrile

MIX 12 50 µg/mL 13C34 Fumonisin B1, 50 µg/mL 13C34 Fumonisin B2

Solid Standards Item No. Product Amount 001006 15-Acetyldeoxynivalenol (98.8 ± 1.2 purity) 5 mg 001106 15-Acetyldeoxynivalenol (98.8 ± 1.2 purity) 10 mg 001003 3-Acetyldeoxynivalenol (99.4 ± 0.6 purity) 5 mg 001103 3-Acetyldeoxynivalenol (99.4 ± 0.6 purity) 10 mg 001012 Aflatoxin B1 (99.5 ± 0.5 purity) 5 mg 001013 Aflatoxin B2 (99.7 ± 0.3 purity) 5 mg 001014 Aflatoxin G1 (98.0 ± 2.0 purity) 5 mg 001015 Aflatoxin G2 (97.1 ± 2.9 purity) 5 mg 001001 Deoxynivalenol (99.4 ± 0.6 purity) 5 mg 001101 Deoxynivalenol (99.4 ± 0.6 purity) 10 mg 001007 Fumonisin B1 (97.6 ± 2.4 purity) 5 mg 001107 Fumonisin B1 (97.6 ± 2.4 purity) 10 mg 001004 Fusarenon X (99.4 ± 0.6 purity) 5 mg 001104 Fusarenon X (99.4 ± 0.6 purity) 10 mg 001010 Neosolaniol (99.4 ± 0.6 purity) 5 mg 001110 Neosolaniol (99.4 ± 0.6 purity) 10 mg 001005 Nivalenol hydrate (98.6 ± 1.4 purity) 5 mg 001105 Nivalenol hydrate (98.6 ± 1.4 purity) 10 mg 001008 Ochratoxin A (99.5 ± 0.5 purity) 5 mg 001108 Ochratoxin A (99.5 ± 0.5 purity) 10 mg 001016 Patulin (99.7 ± 0.3 purity) 5 mg 001017 Sterigmatocystin (99.7 ± 0.3 purity) 5 mg 001011 T-2 Toxin (99.7 ± 0.3 purity) 5 mg on request 001111 T-2 Toxin (99.7 ± 0.3 purity) 10 mg 001009 Zearalenone (99.4 ± 0.6 purity) 5 mg 001109 Zearalenone (99.4 ± 0.6 purity) 10 mg

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003012 Deoxynivalenol in Maize 100 g DON: 2110 ± 290 µg/kg 003017 Fumonisins in Maize 100 g FB1: 2630 ± 740 µg/kg; FB2: 690 ± 340 µg/kg; FB3: 310 ± 210 µg/kg 003018 Fumonisins in Maize 100 g FB1: 270 ± 110 µg/kg; FB2: < 80 µg/kg; FB3: < 80 µg/kg

003023 Ochratoxin A in wheat 100 g OTA 8.6 ± 3.6 µg/kg 003024 Zearalenone und Deoxynivalenol in Maize 100 g ZON: 79 ± 26 μg/kg, DON: 901 ± 110 μg/kg 003025 Deoxynivalenol in wheat DON: 1431 ± 265; Sum of 3AcDON and 15AcDON < 50 100 g

Stable Isotope Labeled Calibrants

ILM006 U-[13C17]-3-Acetyl-Deoxynivalenol (25 µg/mL) in AcN 1.2 mL ILM010 U-[13C17]-Aflatoxin B1 (0.5 µg/mL) in AcN 1.2 mL ILM-011-1.2ML U-[13C17]-Aflatoxin B2 (0.5 µg/mL) in AcN 1.2 mL ILM-012-1.2ML U-[13C17]-Aflatoxin G1 (0.5 µg/mL) in AcN 1.2 mL ILM-013-1.2ML U-[13C17]-Aflatoxin G2 (0.5 µg/mL) in AcN 1.2 mL ILM-021-1.2ML U-[13C17]-Aflatoxin M1 (0.5 µg/mL) in AcN 1.2 mL ILM-020-1.2ML U-[13C19]-Diacetoxyscirpenol (25 µg/mL) in AcN 1.2 mL 002005 U-[13C15]-Deoxynivalenol (25 µg/mL) in AcN 1.2 mL ILM003 U-[13C34]-Fumonisin B1 (25 µg/mL) in AcN/water 1.2 mL ILM004 U-[13C34]-Fumonisin B2 (10 µg/mL) in AcN/water 1.2 mL ILM005 U-[13C34]-Fumonisin B3 (10 µg/mL) in AcN/water 1.2 mL ILM008 U-[13C22]-HT-2 Toxin (25 µg/mL) in AcN 1.2 mL ILM-022-1.2ML U-[13C6]-Kojic Acid (25 µg/mL) in AcN 1.2 mL ILM007 U-[13C20]-Ochratoxin A (10 µg/mL) in AcN 1.2 mL ILM-015-1.2ML U-[13C7]-Patulin (25 µg/mL) in AcN 1.2 mL ILM-014-1.2ML U-[13C17]-Mycophenolic acid (100 µg/mL) in AcN 1.2 mL ILM-019-1.2ML U-[13C15]-Nivalenol (25 µg/mL) in AcN 1.2 mL ILM-016-1.2ML U-[13C22]-Roquefortine C (25 µg/mL) in AcN 1.2 mL ILM-017-1.2ML U-[13C18]-Sterigmatocystin (25 µg/mL) in AcN 1.2 mL 002044 U-[13C24]-T-2 Toxin (25 µg/mL) in AcN 1.2 mL ILM009 U-[13C18]-Zearalenone (25 µg/mL) in AcN 1.2 mL



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003011 Aflatoxins in Maize 55 g AFB1: 7.4 ± 0.37 µg/kg; AFB2: 0.7 ± 0.07 µg/kg; AFG1: < 0.1 µg/kg; AFG2: < 0.1 µg/kg; Aflatoxins total: 8.9 ± 0.26 µg/kg

003014 Deoxynivalenol in Maize 55 g DON: 1840 ± 30 µg/kg 003022 Deoxynivalenol in Wheat 55 g DON: 877 ± 23 µg/kg


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Enzymatic food analysis is a basic method used for the measurement of compounds such as sugars, acids, alcohols and other metabolites in foods and beverages. In general, enzymes are proteins that catalyze the conversion of one molecule into another. Certain enzymatic reactions lead to color formation or consumption that often takes place in a wavelength range invisible to the human eye. This change in intensity can be measured. The high specificity of enzyme reactions allows for the analysis of food components in complex matrices.

Enzymatic methods are especially important for food production and quality assurance. Results give information on the nutrients, authenticity and hygienic status of the food. For example, levels of sugars are crucial for consumers suffering from intolerances and the presence of certain metabolites indicates a lack of hygiene during production. With using enzymatic methods it is also possible to detect whether food has been tampered.

Romer Labs® offers a growing list of enzymatic test kits for rapid and reliable analysis in a microtitre plate format.

The traditional enzymatic method has now been transferred to a microtitre plate kit format –a significant benefit in routine food analysis

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EnzymaticFOOD ANALYSIS

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Enzymatic FOOD ANALYSIS

EnzymeFast® TestsItemNo. Product No.ofAssays

SugarsCOKEF0100 EnzymeFast® Lactose/D-Galactose 140COKEF0200 EnzymeFast® Sucrose/D-Glucose/D-Fructose 140COKEF0300 EnzymeFast® Sucrose/D-Glucose 140COKEF0400 EnzymeFast® D-Glucose/D-Fructose 70COKEF0500 EnzymeFast® Maltose/Sucrose/D-Glucose coming soonCOKEF0600 EnzymeFast® Raffinose/D-Galactose coming soonCOKEF0700 EnzymeFast® Lactose/D-Glucose coming soonCOKEF0800 EnzymeFast® Sucrose/Lactose coming soon

VitaminsCOKEF3000 EnzymeFast® L-Ascorbic acid coming soon

AlcoholsCOKEF6000 EnzymeFast® D-Sorbitol/Xylitol coming soon

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A food allergy is an immune response to a food allergen that the body mistakenly believes is harmful. Food allergies affect 1-3% of the population as a whole and 5-8% of children, making them an important health issue in modern society. Even minimal exposure to a food allergen in the milligram range can cause symptoms from mild skin rashes to fatal anaphylactic shocks.

Despite the fact that any food could provoke an allergic reaction, the vast majority of documented incidents are caused by cereals containing gluten, crustaceans, eggs, fish, peanuts, soybeans, milk and lactose, in addition to nuts. Other allergens such as mustard, sesame, celery, mollusks and lupines may be of more regional importance. Thus, legislation in many parts of the world requires that these ingredients be labeled on food products independent of their concentration.

Allergens might be present as food ingredients, but what is more dangerous is their unexpected appearance via cross-contaminations during the production process. This means testing needs to be done not only by the authorities for labeling compliance, but also by the manufacturer itself for the on-site control of ingredients and finished products.

Romer Labs® provides an extensive range of allergen test kits for laboratory-based, as well as on-site testing.

Food

Eight major foodallergens, theso-called Big 8,are responsible forthe vast majorityof all recorded foodallergy incidents.”

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ALLERGENS

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Food Allergens

G12 AntibodyThe G12 antibody represents the next generation of gluten testing as it targets toxic peptides that trigger auto-immune reactions in people suffering from celiac disease. It is a monoclonal antibody that was raised

against the toxic fragment called 33-mer of the gliadin protein present in gluten. Results obtained from immunochemical test systems based on the G12 antibody should be considered to be closer to the ideal of a food safety test as they establish the important link between celiac disease and detection of the immunotoxic peptides.

ALLERGENS RAPID TESTSAgraQuant® ELISA Tests

Item No. Product Quantitation Range LOD No. of Tests

COKAL0748 AgraQuant® Almond 0.4 – 10 ppm 0.2 ppm 48 COKAL1048 AgraQuant® Beta-Lactoglobulin 10 – 400 ppb 1.5 ppb 48 COKAL1200 AgraQuant® Casein 0.2 – 6 ppm 0.04 ppm 96 COKAL3148 AgraQuant® Cashew 2 – 60 ppm 0.2 ppm 48 COKAL2248 AgraQuant® Crustacea 20 – 400 ppb 0.9 ppb 48 COKAL0848 AgraQuant® Egg White 0.4 10 ppm 0.05 ppm 48 COKAL2548 AgraQaunt® Fish 4 – 100 ppm 1.4 ppm 48 COKAL0200 AgraQuant® Gluten G12 4 – 200 ppm 2 ppm 96 COKAL0248 AgraQuant® Gluten 4 – 120 ppm 0.6 ppm 48 COKAL0348 AgraQuant® Hazelnut 1 – 40 ppm 0.3 ppm 48 COKAL0500 AgraQuant® Histamine 0.5 – 50 ppb 0.15 ppb 96 COKAL0548 AgraQuant® Histamine rapid 3 – 300 ppm 1 ppm 48 COKAL1548 AgraQuant® Lupin 2 – 30 ppm 0.2 ppm 48 COKAL2848 AgraQuant® Lysozyme 25 – 250 ppb 2 ppb 48 COKAL2448 AgraQuant® Milk 0.4 – 10 ppm 0.05 ppm 48 COKAL2148 AgraQuant® Mustard 2 – 60 ppm 1 ppm 48 COKAL2948 AgraQuant® Ovalbumine 25 – 50 ppb 4 ppb 48 COKAL0148 AgraQuant® Peanut 1 – 40 ppm 0.1 ppm 48 COKAL2748 AgraQuant® Pistachio 1 – 40 ppm 0.13 ppm 48 COKAL1948 AgraQuant® Sesame 2 – 30 ppm 0.2 ppm 48 COKAL0448 AgraQuant® Soy 40 – 1000 ppb 16 ppb 48 COKAL0948 AgraQuant® Walnut 2 – 60 ppm 0.35 ppm 48

Accessory COOLS0120 AgraQuant® Allergen Swabbing Kit 20

AgraStrip® Lateral Flow Devices Item No. Product No. of Tests

COKAL0710AS AgraStrip® Almond 10 COKAL1910AS AgraStrip® Brazil Nut 10 COKAL1210AS AgraStrip® Casein 10 COKAL1206AS AgraStrip® Wine Extraction Buffer (Casein) 10 COKAL1310AS AgraStrip® Cashew/Pistachio 10 COKAL1810AS AgraStrip® Egg 10 COKAL1806AS AgraStrip® Wine Extraction Buffer (Egg) 10 COKAL0200AS AgraStrip® Gluten G12 10 COKAL0210AS AgraStrip® Gluten 10 COKAL0310AS AgraStrip® Hazelnut 10 COKAL1510AS AgraStrip® Lupin 10 COKAL1610AS AgraStrip® Macadamia Nut 10 COKAL2110AS AgraStrip® Mustard Kit 10 COKAL0110AS AgraStrip® Peanut 10 COKAL1910AS AgraStrip® Sesame 10 COKAL0910AS AgraStrip® Walnut 10 COKAL2610AS AgraStrip® Coconut 10 COKAL0410AS AgraStrip® Soy 10

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Foodborne illness is a serious public health concern around the world. Foods can become contaminated with pathogenic bacteria through exposure to animal manure, inadequate processing controls, cross-contamination, and improper storage or cooking. Upon ingestion, pathogenic bacteria secrete toxins which can cause human illness, such as diarrhea, fever, vomiting, and abdominal cramps. In severe cases, hemolytic uremic syndrome and listeriosis can lead to death, especially in infants and the elderly.

With the globalization of the food supply it is imperative that food producers test their products for pathogens due to numerous regulatory requirements. Production delays and plant shut-downs, food spoilage, brand protection, and product recalls cost money as well as a company’s reputation; therefore, cost-effective, easy-to-use, and accurate testing methods are necessary to ensure safe food is being supplied to the consumer.

Romer Labs® offers validated food pathogen detection solutions for Escherichia coli O157 and other shigatoxin-producing E. coli, Salmonella spp. (including Salmonella Enteritidis), as well as Listeria spp. in a user-friendly, rapid and accurate, cost-effective format.

Food

A breakdown at any point on the farm-to-table spectrum cancause catastrophicharm to the healthof consumers andgreat disruptionand economic lossto the food industry

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PATHOGENS

Margaret A. Hamburg, M.D.,Commissioner of Food and Drugs

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Food RapidChek® E. coli

ItemNo. Product Quantity

7000157 RapidChek® E. coli O157 Test Kit (no media) 50 Tests7000161 RapidChek® E. coli O157 Media 500 g7000165 RapidChek® E. coli O157 Media 5 kg7000158 Rapid Chek® E. coli O157 Test Kit (no media) 500 Tests7000290 RapidChek® CONFIRM™ non-O157 STEC 100 Tests per O-Type

RapidChek® ListeriaItemNo. Product Quantity

7000171 RapidChek® Listeria Test Kit (no media) 45 Tests7000174 RapidChek® Listeria Environmental System 90 Tests7000175 RapidChek® Listeria Food System 45 Tests7000176 RapidChek® Listeria Media System 500 g/10 g7000238 RapidChek® Listeria F.A.S.T. Test Kit (no media) 45 Tests7000233 RapidChek® Listeria F.A.S.T. Environmental System 135 Tests7000235 RapidChek® Listeria F.A.S.T. Media 500 g

RapidChek® SalmonellaItemNo. Product Quantity

7000191 RapidChek® SELECT™ Salmonella Test Kit (no media) 50 Tests7000190 RapidChek® SELECT™ Salmonella Food System 100 Tests7000195 RapidChek® SELECT™ Salmonella Carcass Rinse System 400 Tests7000198 RapidChek® SELECT™ Salmonella Environmental System 400 Tests7000196 RapidChek® SELECT™ Salmonella Media System 500 g/10 g/250 mL7000183 RapidChek® Salmonella Test Kit (no media) 50 Tests7000167 RapidChek® Salmonella Media 500 g

RapidChek® Salmonella EnteritidisItemNo. Product Quantity

7000222 RapidChek® SELECT™ Salmonella Enteritidis Environmental Swab System 250 Tests7000223 RapidChek® SELECT™ Salmonella Enteritidis Egg Test System 250 Tests7000220 RapidChek® SELECT™ Salmonella Enteritidis Test Kit (no media) 50 Tests7000221 RapidChek® SELECT™ Salmonella Enteritidis Media System 500 g/10 g/250 mL7000225 RapidChek® CONFIRM™ Salmonella Enteritidis 100 Tests

High volume packaging also available

PATHOGENS

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Bovine spongiform encephalopathy (BSE), or “mad cow disease”, is one of several transmissible spongiform encephalopathies (TSEs) or degenerative neurological diseases caused by abnormal prion protein found in the nervous tissues of infected animals that is known to affect various mammalian species including humans. It is spread through the ingestion of infected animal tissue. Most animal health experts believe that the inadvertent contamination of meat and bone meal (MBM) with infected animal tissue and the subsequent use of this material as a feed supplement contribute to the spread of the disease. As a result, the use of processed animal proteins (PAPs) in animal feeds is regulated in many countries around the world.

While feed testing is the only definitive means to certify compliance, regulatory compliance often relies solely on paper certification. Recently, rapid methods have become available for use by regulators to determine compliance during routine inspections. Romer Labs® offers a rapid, cost-effective immunochromatographic strip test for use in the field that can detect 0.1 % MBM in animal feedstuffs (including raw materials and finished feeds).

Ruminant

BSE provides an example of an emerging infectious disease that demons-trates the challengesof policy-making inthe face of rapidly changing scienceand public outrage pushing for action

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MEAT & BONE MEAL

Reference: Hueston, W.D. “BSE and variant CJD: Emerging science, public pressure and thevagariesofpolicy-making.”Preventive

VeterinaryMedicine:2012Dec31.

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RuminantAgraStrip® FeedChek™

ItemNo. Product Quantity

7000201 AgraStrip® FeedChek™ Test Kit 20 Tests7000202 FeedChek™ Sample Buffer 600 mL7000203 AgraStrip® FeedChek™ Test Strips 20 Tests

MEAT & BONE MEAL

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GMO

According to the UN, the global area of GM crop plantation has grown 80-fold between 1996 and 2009.”

“Plants of vital importance to agricul-ture are often genetically modified by the insertion of foreign DNA material into their DNA sequence, resulting in expressed novel traits that normally would not appear in nature -- typically herbicide tolerance or insect resistance. Seeds harvested from GMO plants will also contain the modification.

Current GMO production basically cent-ers on four main crops: soybeans, corn, cotton and oilseed rape. On a global level, GMO crops are cultivated on sub-stantial areas of arable land. Approxi-mately 90% of all soy available com-mercially is GMO soy (ca. 80% of corn, ca. 70% oilseed rape and almost 50% of cottonseed).

When testing for GMOs, detection is achieved either through the inserted DNA or expressed protein translated from the novel DNA. The inserted DNA and its corresponding protein are found in all the cells of the plant. Thus, the seeds and/or plant tissue material (e.g. leaves) can be used for GMO testing.

Testing for the expressed protein can be performed using rapid on-site test kits, such as lateral flow devices (LFD) or ELISA, while the reference method for detecting the DNA itself -- using poly-merase chain reaction (PCR) methods -- needs to be done by specialized labs. Romer Labs® offers a wide range of rap-id tests, based on LFD and ELISA tech-nologies, covering the majority of reg-istered GMO traits found on the market, in cooperation with SDIX.

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GMORAPID TESTS

AgraQuant® ELISA Tests Item No. Product Trait Range No. of Tests

GMOChek™ 7020000 AgraQuant® Cry1F SeedChek™ Bt-Cry1F n/a 960 7020100 AgraQuant® EPSPS SeedChek™ CP4 EPSPS n/a 960 7020105 AgraQuant® Vip3A SeedChek™ Vip3A n/a 960 7140220 AgraQuant® DAS Cry1Ac SeedChek™ Bt-Cry1Ac n/a 960 7099999 AgraQuant® Soya Toasted Meal GMOChek™ CP4 EPSPS 0.3 – 2.5 % 96 7100000 AgraQuant® RUR Soya Grain GMOChek™ CP4 EPSPS 0.3 – 2.5 % 96 7110000 AgraQuant® Cry1Ab Maize GMOChek™ Bt-Cry1Ab 0.15 – 2.0 % 96 7110030 AgraQuant® Cry9C Maize GMOChek™ Bt-Cry9C 0.0075 – 0.10 % 96

Standards 7100001 RUR Soya Standards - Full Fat Flour (for flour, meal, and grit) 7100002 RUR Soya Standards - Defatted Flour 7100004 RUR Soya Standards - Protein Isolate

AgraStrip® Lateral Flow Devices Item No. Product Trait No. of Tests

SeedChek™ 3000007 AgraStrip® Cry2Ab Bt-Cry2Ab 50 3000008 AgraStrip® LL Cotton PAT 50 3000091 AgraStrip® EPSPS EPSPS (Event GA21) 50 3000094 AgraStrip® Cry1Ac Bt-Cry1Ac 50 7000052 AgraStrip® PMI PMI 50 7800010 AgraStrip® RUR CP4 EPSPS 50 7800011 AgraStrip® Cry3Bb Bt-Cry3Bb 50 7800012 AgraStrip® Cry34Ab1 Bt-Cry34Ab1 50 7800013 AgraStrip® RUR-HS CP4 EPSPS 50 7800014 AgraStrip® Triple Trait CP4 EPSPS, 50 Bt-Cry1Ab, Bt-Cry3Bb 7800015 AgraStrip® Cry1F Bt-Cry1F 50 (water extract) - corn 7800016 AgraStrip® Vip Vip3A 50 7800017 AgraStrip® Cry1Ab Bt-Cry1Ab 50 7800018 AgraStrip® Cry1F Bt-Cry1F 50 (buffer extract) - cotton 7800019 AgraStrip® LL PAT 50 7802000 AgraStrip® Btk Bt-Cry1Ab, Bt-Cry1Ac 50 7803000 AgraStrip® BR CP4 EPSPS, Bt-Cry1Ac 50 7805000 AgraStrip® B2R CP4 EPSPS, 50 Bt-Cry2Ab, Bt-Cry1Ac

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GMORAPID TESTS

AgraStrip® Lateral FLow Devices Item No. Product Trait LOD No. of Tests

TraitChek™ 7000011 AgraStrip® RUR-HS Bulk Grain CP4 EPSPS 0.125 %in corn 100 0.1 %in canola 0.167 %in alfalfa 7000012 AgraStrip® Cry9C Bulk Grain1 Bt-Cry9C 0.125 %in corn 100 7000014 AgraStrip® RUR Bulk Grain CP4 EPSPS 0.1 %in soy and 100 sugarbeets in 5 min; 0.143 % in soy in 3 min 7000017 AgraStrip® RUR Seed and Leaf CP4 EPSPS qualitative 100 7000018 AgraStrip® Cry1F Bulk Grain Bt-Cry1F 0.9 %in corn 100 (buffer extract) 7000025 AgraStrip® Cry1Ab Bulk Grain Bt-Cry1Ab 0.9 %in corn 100 7000026 AgraStrip® Cry1Ab Seed and Leaf Bt-Cry1Ab qualitative 100 7000028 AgraStrip® Cry1F Seed and Leaf Bt-Cry1F qualitative 100 7000041 AgraStrip® Cry3Bb Bulk Grain Bt-Cry3Bb 0.125 %in corn 100 7000042 AgraStrip® Cry3Bb Seed and Leaf Bt-Cry3Bb qualitative 100 7000043 AgraStrip® LL Bulk Grain PAT 0.9 %in corn and 100 sugarbeet, 2 %in canola 7000044 AgraStrip® Cry1Ab/LL Bulk Grain Combo Bt-Cry1Ab, PAT 0.9 %in corn 100 7000045 AgraStrip® LL Seed and Leaf PAT qualitative 100 7000048 AgraStrip LL Bulk Grain for Rice PAT 0.05 %in LLRice62 100 2 %in LLRice61 7000053 AgraStrip® Cry1F Bulk Grain Bt-Cry1F 0.9 %in corn 100 (water extract) 7000054 AgraStrip® Triple Trait Seed and Leaf CP4 EPSPS, qualitative 100 Bt-Cry1Ab, Bt-Cry3Bb 7000055 AgraStrip® Cry34Ab1 Bulk Grain Bt-Cry34Ab1 0.125 %in corn 100 7000092 AgraStrip® Vip Seed and Leaf Vip3A qualitative 100 7000093 AgraStrip® Vip Bulk Grain Vip3A 0.33 %in corn 100 7000094 AgraStrip® Cry2Ab Seed and Leaf Trait Check Bt-Cry2Ab qualitative 100 7120050 AgraStrip® RUR Toasted Meal CP4 EPSPS 0.9%in toasted soy 100

7806000 AgraStrip® Triple Trait Bulk Grain CP4 EPSPS, 0.5 %in corn, 100 Bt-Cry1Ab, 0.9 %in corn, Bt-Cry3Bb 0.5 % in corn 7880610 AgraStrip® Corn Comb 7 Traits CP4 EPSPS depends on 20 PAT, Bt-Cry9C the trait Bt-Cry1Ab, Bt-Cry3Bb, Bt-Cry1F Bt-Cry34Ab1 7880623 AgraStrip® Soy Comb 2 Traits CP4 EPSPS, PAT depends on 20 the trait

1USDA/GIPSA approved

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GMORAPID TESTS

AgraStrip® Lateral Flow Devices ItemNo. Product Trait Range Commodity No.of Tests

Semi-quantitative TraitChek™ 7000011 AgraStrip® RUR-HS Bulk Grain CP4 EPSPS 0.1 to 4.0 % corn 100 7000012 AgraStrip® Cry9C Bulk Grain Bt-Cry9C 0.1 to 4.0 % corn 100 7000014 AgraStrip® RUR Bulk Grain CP4 EPSPS 0.1 to 4.0 % soy 100 7000025 AgraStrip® Cry1Ab Bulk Grain Bt-Cry1Ab 0.5 to 4.0 % corn 100 7000041 AgraStrip® Cry3Bb Bulk Grain Bt-Cry3Bb 0.1 to 4.0 % corn 100 7000043 AgraStrip® LL Bulk Grain PAT 0.5 to 4.0 % corn 100 7000053 AgraStrip® Cry1F Bulk Grain Bt-Cry1F 0.1 to 4.0 % corn 100 (water extract) 7000055 AgraStrip® Cry34Ab1 Bulk Grain Bt-Cry34Ab1 0.1 to 4.0 % corn 100 7000093 AgraStrip® Vip Bulk Grain Vip3A corn 100 7880620 AgraStrip® Semi quantitative CP4EPSPS, PAT depends corn 20 Corn comb Vip 3A, Bt-Cry1Ab, on the trait Bt-Cry9C, Bt-Cry3Bb Br-Cry1F, Bt-Cry34Ab1 7880622 AgraStrip® Semi quantitative CP4EPSPS, PAT, Vip 3A, depends corn 20 Corn Comb for Bt-Cry1Ab, Bt-Cry9C, on the trait AgraVision™ Reader Bt-Cry3Bb, Bt-Cry1F, Bt-Cry34Ab1

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& ADULTERANTS

Residues in food and feed are a very heterogeneous group of substances ranging from process contaminants to pesticides, veterinary drugs or even substances added illegally, such as melamine.

Veterinary drugs are used in animal production to treat disease, maintain herd and flock health, promote growth, improve meat or otherwise reduce production costs. There is a possibility that residues or their metabolites may appear in the final animal-based foods with their use. Government authorities control the use of veterinary drugs and monitor food for unsafe or prohibited residues. There are a wide variety of regulations in place, with some veterinary drugs allowed in animal products for food production under certain conditions and others banned from use completely.

Melamine, widely used in combination with formaldehyde to produce melamine resin and melamine foam for countertops, glues, household goods and flame retardants, has been added to raw milk and feed illegally to give the appearance of higher protein content than the actual level. Melamine itself is non-toxic in low doses, but can cause fatal bladder cancer and kidney stones in combination with other chemicals.

Pyrrolizidine alkaloids, plant toxins widely found in the Asteraceae, Boraginaceae and Fabaceae families, can form toxic compounds when metabolized (e.g. in the liver). Several food products such as milk, honey, eggs and herbal teas, as well as medicinal products and animal feed, have been found contaminated with pyrrolizidine alkaloids. Romer Labs® offers ELISA-based test kits, standards and reference materials for residues.

Residues

In contrast topesticides, exposureto veterinary drugresidues is rather morecommon, as they aredirectly injected orfed to the animals.”

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& ADULTERANTSVeterinary

DRUG RESIDUESAgraQuant® ELISA Tests

Item No. Product Quantitation Range LOD No. of Tests

COKDA1100 AgraQuant® Chloramphenicol 0.025 – 10.0 ppb 0.03 – 0.031 ppb* 96 COKDA0200 AgraQuant® AMOZ 0.2 – 16.2 ppb* 0.2 ppb 96 COKDA0300 AgraQuant® AOZ 0.1 – 3 ppb* 0.2 ppb 96 COKDA0400 AgraQuant® Clenbuterol 0.15 – 20.0 ppb* 0.054 – 1.094 ppb* 96 COKDA0500 AgraQuant® Ractopamine 0.3 – 50.0 ppb* 0.21 – 3.53 ppb* 96 COKDA0600 AgraQuant® Beta-Agonists 0.5 – 50.0 ppb* 0.28 – 0.51 ppb* 96 COKDA0800 AgraQuant® Dexamethasone 0.15 – 25.0 ppb* 0.05 – 0.36 ppb* 96 COKDA0900 AgraQuant® Ciprofloxacin 3 – 360 ppb* 0.73 - 2.9 ppb 96 COKDA1300 AgraQuant® Nitrofuran (AHD) 0.3 – 16 ppb 0.24 – 0.37 ppb* 96 COKDA1200 AgraQuant® Quinolones 2 – 500 ppb 1.14 – 1.87 ppb* 96 COKDA1400 AgraQuant® Nitrofuran (SEM) Coming soon COKDA1500 AgraQuant® Nifursol Coming soon COKDA1600 AgraQuant® Sulfonamides Coming soon COKDA1700 AgraQuant® Cimaterol Coming soon

*depending on matrix


Calibrant Solutions ANT-005-5ML Chloramphenicol (100 µg/mL) in acetonitrile 5 mL ANT-004-5ML Cyclosporine A (100 µg/mL) in acetonitrile 5 mL ANT-002-5ML Griseofulvin (100 µg/mL) in acetonitrile 5 mL ANT-007 Tetracycline (2.5 µg/mL) dried down 5 x 1mL ANT-009 Oxytetracycline (2.5 µg/mL) dried down 5 x 1mL

Stable Isotope Labeled Calibrants ANT-006-1.2ML U-[13C11]-Chloramphenicol (25 µg/mL) in acetonitrile 1.2 mL ANT-003-1.2ML U-[13C62]-Cyclosporine A (25 µg/mL) in acetonitrile 1.2 mL ANT-001-1.2ML U-[13C17]-Griseofulvin (25 µg/mL) in acetonitrile 1.2 mL

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ResiduesOTHER

AgraQuant® ELISA Tests Item No. Product Quantitation Range LOD No. of Tests

Melamine COKAQ9300 AgraQuant® Melamine 2 – 50 ppm* – 96 COKAQ9400 AgraQuant® Melamine Sensitive 0.1 – 5 ppm* 0.008 ppm* 96

*depending on matrix


Calibrant Solutions ALK-001-1ML Atropine (100 µg/mL) dried down 1 mL ALK-003-1ML Scopolamine (100 µg/mL) dried down 1 mL ALK-004-1ML Hyoscyamine (100 µg/mL) dried down 1 mL ALK-002-1ML Monocrotaline (100 µg/mL) dried down 1 mL ALK-005-1ML Retrorsine (50 µg/mL) dried down 1 mL ALK-006-1ML Senecionine (50 µg/mL) dried down 1 mL ALK-008-1ML Seneciophylline (50 µg/mL) dried down 1 mL ALK-007-1ML Senkirkine (50 µg/mL) dried down 1 mL CMT001 Melamine (100 µg/mL) in acetonitrile 5 mL

Stable Isotope Labeled Calibrants CMT002 U-[13C3]-Melamine (100 µg/mL) in acetonitrile 1.2 mL

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Analyzing samples in your own laboratory often raises the question of comparability. How do the results generated compare to other labs using identical samples? The most common way to find out is by taking part in a proficiency testing program or a check sample survey. In such program, participants receive homogenized sample material to be analyzed using in-house laboratory methods. A description of the procedure and the results will be then forwarded to the organizer, following which the results will be compiled and analyzed statistically. Every participant will get a final report, accompanied by an anonymous summary of all results, together with their own individual results. Romer Labs® organizes such programs four times a year for mycotoxins and anyone may take part on a worldwide basis. Naturally contaminated material, homogenized and characterized, will be used as samples for the check sample survey.

More details on availability and planned rounds, the type of mycotoxins and matrices in the program, as well as examples of reports from previous rounds, can befound on our website(http://www.romerlabs.com) or are available from your local Romer Labs® representative or distributor.

Check Sample Survey

Participating in a sample survey, a multi-laboratory study aimed at producing summary statistics, will give you confidence in your own results.”

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Thirty years of experience has built confidence and competence in the Romer Labs® analytical testing service as a partner for accurate and reliable results.

The service operates on a global scale with laboratories in Austria, Singapore, the United Kingdom and the United States. Comprehensive analytical services for mycotoxins, allergens, GMOs, veterinary drug residues and other contaminants that are of key relevance in food and feed safety are available. These services are on offer for a variety of sample matrices, including grains, feed samples, food samples and many others.

Reliable and accurate results are established through ISO9100 certification and ISO17025 accreditation, as well as other reference lab certifications and participation in official proficiency testing programs. State-of-the-art equipment allows our highly trained and motivated analysts to use the latest methods and technologies to perform analyses with the highest level of accuracy and minimal turn-around time.

Well thought-out quality control systems are important, but it is the analytical data that help to make decisions for fulfillment of quality.”

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ISO

9 1 0 0

ISO

17 0 25

AnalyticalSERVICE

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AnalyticalSERVICE

Analytical Service

Romer Labs® is offering Analytical Services in its laboratories in Austria, Singapore, United States and United Kingdom. The following is a list of parameters and their availability in the respective labs.

Analyte Austria Singapore USA UK

Mycotoxins

3-Acetyl DON X X 15-Acetyl DON X X Aflatoxin B1, B2, G1,G2 X X X XAflatoxin M1 X X X Citrinin X Cyclopiazonic Acid X DAS X X DON X X X XErgot Alkaloids X X X (Ergocornin, Ergocorninine, Ergocristine, Ergocristinine, Ergocryptin, Ergocryptinine,Ergometrin, Ergometrinine, Ergosin, Ergosinine, Ergotamin, Ergotaminine)Ergocornine X X Ergocristin X X Ergocryptin X X Ergosin X X Ergotamin X X Fumonisin B1, B2, B3 X X X XFusarenon X X X HT-2 Toxin X X Moniliformin X Neosolaniol X Nivalenol X X Ochratoxin A X X X XPatulin X X Sterigmatocystin X T-2 Toxin X XTrichothecenes A X X X (Neosolaniol, DAS, T-2 Toxin, HT-2 Toxin)Trichothecenes B X X X (DON, 3-Acetyl-DON, 15-Acetyl-DON, Nivalenol, Fusarenon X)Zearalenol Zearalenone X X X Mycotoxin Binder Study X X X X

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AnalyticalSERVICE

Analytical Service


Contaminants

Ammelide X Ammeline XCyanuric Acid X Melamine X X X

Food Allergens

Almonds X X X XBLG (ß-Lactoglobulin) X X X XBrazil Nut XCasein X X X XCashew X X XCelery X XCrustacea X X XEgg White X X X XGluten X X X XHazelnuts X X X XHistamine X X XLactose XLupin X X XMolluscs XMustard X X X XPeanuts X X X XPistachio X X XSesame X X X XSoy X X X XSulphite XWalnut X X X X

Drug Residues

Beta-Agonist X XChloramphenicol X X Clenbuterol X X Nitrofuran X XRactopamine X X

Pesticide Screening

Carbamates X DithioCarbamates X Organochlorus(OC) X Organophosporus (OP) X Pyrethroids X

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Analytical Service


GMOs

Roundup Ready Soy X XBt-11 Maize X XBt-176 Maize X XGA21 Maize X XMON810 Maize X XNK603 Maize X XStar Link Maize X XT25 (Liberty Link) Maize X XMON863 Maize X E3272 Maize X DP98140 Maize X Bt-10 Maize X TC1507 (Herculex) Maize X MON89788 Soy X DP-305423-1 Soy X DP-356043-5 Soy X BPS-CV127-9 Soy X A2704-12 Soy X RT73 Rapeseed X T45 Rapeseed X BT63 Rice X LL601 Rice X FP967 Lineseed X EH92-527-1 Potato X GMO Screening X X

Speciation

Beef XPork XChicken XTurkey XSheep XCod XHaddock XAtlantic salmon X

For further details, please contact Romer Labs sales or the respective lab.

• Romer Labs Inc. in Union, MO, USA - [email protected]• Romer Labs Diagnostic GmbH. in Tulln, Austria - Quantas Analytik - [email protected]• Romer Labs Singapore Pte. Ltd, Singapore - [email protected]• Romer Labs® UK Ltd. in Runcorn, Cheshire, UK - [email protected]

AnalyticalSERVICE

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When performing an analytical test, strong focus has to be put on the equipment used to obtain accurate and reproducible test results. The first step of any analysis is the use of a well-established sampling plan to obtain a representative sample. After taking the representative sample – for instance, consisting mostly of several aggregated subsamples taken out of a large storage tank – appropriate sample preparation is vital.

This lab sample needs to contain the same concentration of the analyte as the sample from which it was taken. To achieve this goal, two steps are required. First, the representative sample needs to be ground and then a small analytical sample needs to be taken. This is done by using equipment such as mills and/or subsampling devices.

Romer Labs® offers a range of equipment for such analytical purposes, including Romer Mills for grinding and sample division and the Romer Evap® System for the rapid concentration of sample extracts.

Furthermore, quantitative and/or semi-quantitative analyses often require additional equipment for result interpretation. For instance, Romer Labs® offers the AgraVision for lateral flow test interpretation and StatFax or ChroMate from Awareness Technology, Inc. for ELISA quantification purposes.

Last but not least, aflatoxin analysis by HPLC-FLD requires derivatization of the analytes for higher sensitivity; a task made possible by Romer Labs® photo chemical or electrochemical derivatization units.

High quality equipment is essential to obtain accurate analytical results.”

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Equipment

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EquipmentLaboratory Equipment

Item No. Description

Romer Mills Romer Series II® Mill7

EQMMS2010 Series II® Mill, 115V EQMMS2015 Series II® Mill, 230V, American Plug EQMMS2018 Series II® Mill, 230V, European Plug The patented Romer Series II® Mill can prepare a representative sample

quickly and easily. A 2 lb. (1 kg) sample of corn can be ground and subsampled within 1 to 2 minutes. This mill simultaneously grinds and splits the samples and is the only mill available that combines these two steps into one piece of equipment. The Series II® Mill is USDA/GIPSA

approved and is used mainly for grains, feeds and foods.

RAS® (Romer Analytical Sampling) Mill7

EQMMR1010 RAS® Mill, 115V EQMMR1017 RAS® Mill, 230V The RAS® Mill has been specifically developed for products that are difficult

to grind due to high moisture and/or high oil content. Among these products are cottonseed, tree nuts, peanuts and pistachios. A 2 lb. (1 kg) sample of corn can be ground and subsampled in 1 minute. Special features include the availability of 2 unique chute options (to allow more rapid and accurate grinding and sampling), interchangable grinding burrs, and power drive screws to allow the grinding of practically any product. The moving parts are made of stainless steel and flashed chrome for food safety analyses. The RAS® Mill is ideal for use in all labs where grinding and subsampling is

essential, from pesticides to mycotoxins, moisture and proteins.

Reader StatFax ELISA Reader (Awareness Technology, Inc.) EQOLE1409 Stat Fax 303 ELISA Reader The StatFax ELISA Reader is a microstrip reader intended for in-vitro diagnostic

use. It is a compact, microprocessor-controlled, photometer system designed to read and calculate the results of endpoint colorimetric assays, which are read in microtiter strips. All AgraQuant® ELISA test kits featuring breakaway

strips can be read by a Statfax ELISA Reader.

ELx800 UV Reader (BioTek Instruments Inc.) EQOLE1495 BioTek® ELx800 UV Absorbance Microplate Reader The ELx800 UV is a compact microplate reader that measures in the UV as

well as in the visible range. The standard filters 340, 405, 490 and 550 nm are included. It reads 96 well microplates in 30 seconds and comes with Gen5 RC Software. All EnzymeFast® Test Kits can be read using the ELx800 UV reader.

ChroMate ELISA Reader (Awareness Technology, Inc.) EQOLE1408 ChroMate ELISA Reader The ChroMate ELISA Reader is a compact and economical 8-channel

microplate reader, intended for in-vitro diagnostic use. It reads a 96 well plate in 12 seconds and comes with versatile and flexible software. All AgraQuant®

ELISA test kits can be read with the ChroMate ELISA Reader.

7 AOAC Official Method, USDA/GIPSA approved, UNECE Standard, FAO recommended, OECD recommended

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EquipmentLaboratory Equipment

Item No. Description

Reader

FQReader EQFFM3000 FQReader EQFFM3010 FQReader with Printer and Accessories The Romer Labs® FQ-Reader allows fluorometric detection and quantification

of mycotoxins and is used in conjunction with Romer Labs FluoroQuant® Test Kits. It is a sensitive, easy-to-use laboratory instrument which measures the fluorescence in samples and calculates ppm or ppb utilizing customized data processing and calibration routines. This provides accurate ppm or ppb results. A thermal printer is available for data print out, or the FQ-Reader can

transfer data directly to a computer.

AgraVision™ EQASR1000 AgraVision™ with printer EQASR1003 AgraVision™ The AgraVision™ reader is Romer Labs® handheld lateral flow device reader

used for the quantification of AgraStrip® Mycotoxin and GMO products. It measures the intensity of the test line on the AgraStrip® and can read up to two mycotoxin strips or 4 GMO strips simultaneously. With the optional AgraVision™ printer strip test results can easily be stored for hard copy documentation.

Romer LogTag Temperature Recorder

ROMTrix-8 Romer Labs LogTag Device ROMLTI/USB USB Interface The Romer LogTag device to record environmental temperatures is an es-

sential tool for laboratories. Recording temperature profiles is crucial for laboratories that are required to monitor environmental temperatures of i.e. refrigerators or warehouses as part of their accreditation status.

Romer Evap® System EQOEV1030 Romer Evap® System, 12 Port, small, 115V EQOEV1033 Romer Evap® System, 12 Port, large, 115V EQOEV1040 Romer Evap® System, 24 Port, small, 115V EQOEV1043 Romer Evap® System, 24 Port, large, 115V EQOEV1031 Romer Evap® System, 12 Port, small, 230V EQOEV1032 Romer Evap® System, 12 Port, large, 230V EQOEV1041 Romer Evap® System, 24 Port, small, 230V EQOEV1042 Romer Evap® System, 24 Port, large, 230V The Romer Evap® is a versatile easy-to-use system that rapidly evaporates

common organic solvents. Among the many features of the Romer Evap® System are the stainless steel design and solvent-resistant tubing. The system can be customized for either 12 or 24 port evaporation. Each row of ports can be separately controlled to regulate the amount of vacuum or may be completely shut-off when not in use. The Romer Evap® System includes a

12 or 24 port evaporator, dry bath and vacuum pump.

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Equipment

TLC Autospotter™ EQOAS1010 TLC Autospotter®, 115V EQOAS2010 TLC Autospotter®, 230V The Romer Labs® TLC AutoSpotter reduces the time traditionally associated

with TLC applications. It allows accurate dispensing of contents of a 100-µL syringe within 3 minutes and assures tight uniform spots for accurate test results. The AutoSpotter is equipped with eighteen channels for maximum testing capabilities. Special features and calibrated controls

provide accurate reproducible results every time.

RDU™ EQOLE1480 Romer® Derivatization Unit The Romer® Derivatization Unit (RDU™) is a photochemical post-column

derivatization for the determination of aflatoxins by HPLC-FLD. For sensitive determination of aflatoxins, the aflatoxins B1 and G1 must be derivatized in order to enhance their natural fluorescence. This may be done with ease photochemically by irradiation with UV light at 254 nm with the RDU™. The method performance is equivalent to an electrochemical derivatization (e.g. Kobra Cell) as shown in the literature. Addtionally, by using the RDU™ there is no necessity for additional purging and lifetime of the HPLC system is increased due to the fact the RDU™ doesn’t require any aggressive reagents to be added to the mobile phase, as this is the case for electrochemical methods.

Romer® Cell EURCO1080 Romer® Cell The unit enables electrochemical post-column derivatization for the determination of aflatoxins by HPLC-FLD.

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CATALOG 2013 - foodriskmanagement.com...Technopark 1, A-3430 Tulln, Austria Tel: +43 2272 615 3310...

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