Carolina Biological Supply...
Transcript of Carolina Biological Supply...
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2012 NABT
Carolina Biological Supply Company
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• Conduct a pig dissection using the protocol for a human autopsy
• Learn an exciting approach to a classic mammalian dissection
• Experience the quality of Carolina’s Perfect Solution® specimens
Objectives
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Carolina™ Forensic Dissection Kit
Workshop features activities from one of our most popular dissection kits!
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Excerpt from Kit Manual
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Excerpt from Kit Manual
Students work in
groups of 4.
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We will work in groups of 2.
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Why Are We Doing a Pig AUTOPSY?
• Pig anatomy is very similar to human anatomy
• Organ systems are completely removed and studied
• Unique protocol that your students will enjoy
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Carolina’s Perfect Solution® Specimens
Superior preservation
Superior tissue color and texture
Quality No dangerous off-gassing
No formalin odor
Safety
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“I use pig dissection as a teaching tool because the anatomy and physiology of the pig is so similar to man. Carolina’s Perfect Solution® pigs are the finest on the market.” —Dr. David Whetstone Biology Department Jacksonville State University
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Safety Issues
• Personal Protective Equipment Gloves, goggles, and lab aprons
• Dissection Tools New tools = sharp scalpels
• Safety Tip If you are not using an
instrument, set it down
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Model Proper Lab Safety!
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Ready?
Lay out your instruments so they are easy to
access.
Organize your dissection area.
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Teacher Tip Use rubber bands instead of string
to secure pigs in pans.
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Teacher Tips
Sponge used as “body block”
Rubber bands used to hold
pigs in position
White pad placed in pan for easy cleanup
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Teacher Tips
Hold bag upright.
Cut below the seal.
Allow fluid to drain into the bag before
removing pig.
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Twist 1 side of rubber band around 1 leg.
Wrap around back of tray, and twist around other leg.
Secure Your Pig
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Teacher Tip Double-loop the rubber
band around the ankle to keep it from slipping off.
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Male Female
Urogenital opening
Scrotum Genital papilla
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External Anatomy
• Hair • Mouth • Nostrils • Tongue • Ears • Eyes
Examine external features of the head:
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Student groups perform a thorough
external examination of the pig.
Internal structures are studied next.
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Data is captured in an autopsy
report.
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Prepare for Internal Anatomy
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Feel for the space between the top of the shoulder and the corner of the jaw. This will assist you in making
the initial incision.
First Incision
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Classic Y Incision
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Y-Shaped Incision
Continue cutting the tail of the Y incision until you
reach the umbilical cord.
Use appropriate cut for male or female pig.
Cut the skin, muscle, and connective tissues of the chest wall (exposing the sternum and rib cage).
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Lift the V-shaped flap and pull it gently upwards. Use the tip of the scalpel, as shown, to assist in lifting the flap.
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Thymus gland tissue: Tease away to expose the thyroid gland below, lying between these 2 masses.
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. The thymus gland tissue is removed,
and the thyroid gland is exposed.
Thyroid gland
Trachea
Thyroid cartilage over larynx
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Remove thymus and thyroid tissue.
Measure the length and mass, and record them on your data table.
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Peel skin and tissue away from midline incision. Use the scalpel or scissors to help clear away
the fascia. Make a lateral incision on both sides of the body at the bottom of the rib cage.
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Umbilical vein exposed
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Make low, lateral incisions to expose the abdominal cavity.
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Cut up each side of the rib cage to remove the chest plate. Stay
low in the chest cavity.
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The diaphragm may still be attached at the bottom of the rib cage after cutting up both sides of the rib cage. Cut the
diaphragm away now, so the chest plate can be lifted.
Diaphragm
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The diaphragm is cut away.
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Cut away the remaining connections of tissue and bone. Lift the chest plate off.
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Before removing the organ block, have students look for and examine the pancreas. Gently lift the stomach
and look for the gland tissue in the crease between the bottom
of the stomach and the top of the intestines.
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Stomach, lifted
Pancreas
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To begin removal of the organ block, make the initial incision at the top of the trachea, just below the larynx.
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Lift from trachea and pull gently downward. Make sure you have included the esophagus lying posterior to the trachea.
Use the tip of the scalpel to help remove the connective tissue that holds the organ block to the back of the body.
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After beginning the initial removal of the organ block, go to the
lowest point of the large intestine and make an incision. Proceed with removal
of the organ block from the bottom by pulling gently upward.
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Make incision here.
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As you pull upward, note the kidneys in the posterior
of the abdominal cavity. These can be removed later.
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Kidneys
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Organ Block Removed
Begin to remove organs
individually.
Measure the length and mass of each organ.
Record on data table.
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The gallbladder is embedded in the lobe of liver on the underside.
Lift gently to expose common bile duct.
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Internal Anatomy
• Respiratory system • Endocrine system • Circulatory system • Digestive system • Urogenital system • Fetal pig heart vs. adult pig heart • Fetal pig kidney vs. adult pig kidney • Central nervous system (brain, spinal cord) • Prepared microscope slides
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Bringing Home the Bacon!
Return all of the organs to the body cavity and suture the incisions.
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Cleanup Instructions
• KEEP GLOVES ON!
• Place ONLY animal waste in buckets.
• All other trash goes in trash bags.
• Wipe out pans, clean tools, and wipe off tables.
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Carolina Free Resources
Carolina offers many free resources to help support teachers.