NIH Public Access Kenneth Hayworth Richard Schalek Jeff W ...
Can we extract a mind from a plastic-embedded brain? - Kenneth Hayworth - H+ Summit @ Harvard
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Transcript of Can we extract a mind from a plastic-embedded brain? - Kenneth Hayworth - H+ Summit @ Harvard
Can we extract a mind from a plas3c-‐embedded brain?
Kenneth Hayworth, PhD
June 12, 2010
4.5mm
1.2mm
Mouse brain
38nm thick mouse cortex
Cor2cal surface
Layer 4 axons
Layer 2,3 cell bodies
Images thanks to Bobby Kasthuri, Richard Schalek, Juan Carlos Tapia, Daniel Berger and Jeff Lichtman
Automatic Tape-collection Mechanism
Automatic Tape-collection Mechanism
~3mm
30nm stack
200Sections_30nmCortex_Uncompressed_7fps.avi
FEI DualBeamFocused Ion Beam + Scanning Electron Microscope
“Serial Sec;on Scanning Electron Microscopy of Adult Brain Tissue Using Focused Ion Beam Milling” (KnoD et al. 2008 )
FIBSEM Stack
Movie from “Serial Sec;on Scanning Electron Microscopy of Adult Brain Tissue Using Focused Ion Beam Milling”(KnoD et al. 2008 )
200 – 300 micron thick sec;on of newborn mouse brain
Same thick sec;on with blocks removed for ultrathin sec;oning and TEM reconstruc;on.
2 + 2 = 4
www.BrainPreserva3on.org