CAMAG TLC VISUALIZER 2

WORLD LEADER IN PLANAR CHROMATOGRAPHY

Professional Imaging and Documentation System for TLC/HPTLC Chromatograms with a new

Digital CCD Camera, connected by USB 3.0

The visual presentation of the complete chromatogram showing all samples and standards side by side is one of the most con- vincing arguments for Thin-Layer Chromatography. No other chromatographic technique can directly express the result as a color image and make it available for visual evaluation.

To reproducibly acquire and preserve best quality images of TLC/HPTLC chromatograms under different illumination modes this high-end imaging and documentation system is now available. With its new digital CCD camera a maximum resolution of 82 µm on the plate is obtained.

For electronic image acquisition the camera – like the human eye – captures polychromatic visible light. Under white light illumination it is the light reflected from the layer background. Under long-wavelength UV light (366 nm) it is the light emitted by fluorescent substances. When short-wavelength UV light (254 nm) is used, substances absorbing UV 254 nm appear as dark zones, provided the layer contains a fluorescence indicator (fluorescence quenching).

Visualization, Evaluation, and Archiving

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Chromatogram under UV 366 nm

Chromatogram under white light

Chromatogram under UV 254 nm

Reproducible high-quality images acquired under homogenous illumination with the selected light

Easy and intuitive operation with visionCATS

High-dynamic-range imaging (HDRI)

Side by side comparison of tracks originating from the same or different plates and/or different illumination modes

Various image enhancement tools, e.g. “Spot Amplification”, “Clean Plate Correction” and “Exposure Normalization”

Image-based profile generation from reference and sample tracks, and subsequent peak integration and calibration

New digital CCD camera with a maximum resolution of 82 µm on the plate

USB 3.0 for easy PC connection

Meets all requirements to be used in a cGMP/cGLP environment

IQ/OQ qualification and 21 CFR Part 11 ready

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Key Features

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“Data View” allows for visual evaluation of a plate in different illumination modes and offers a broad range of helpful tools, e.g. the RF tool displays the RF value of zones on the digital image or a rectangular zoom function.

visionCATS organizes the workflow of TLC/HPTLC, controls the involved CAMAG instruments, and manages data. Professional image acquisition and documentation of TLC/HPTLC chromato- grams ensuring highest reproducibility is the major purpose of the TLC Visualizer 2.

For the evaluation of acquired images sophisticated enhance- ment tools and functions for annotation and determination of position (RF) are required.

This is where visionCATS comes into play and virtually unleashes the power of TLC/HPTLC. visionCATS features a powerful data- base at its heart, enabling an all new sample-based approach. The state-of-the-art software supports low-noise, high-dynamic-range imaging (HDRI) and includes a comprehensive set of image enhancement tools. With the “Comparison Viewer”, tracks originating from the same or different plates and/or different illumination modes can be compared on the same screen side by side which allows the creation of “virtual plates“. Detailed on-screen instructions effectively guide the user through the image acquisition process.

“Comparison Viewer“: selected tracks of images taken from different plates under UV 366 nm, UV 254 nm, and white light can be displayed side by side.

CAMAG TLC Visualizer 2 operated under visionCATS

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Image Enhancement

The image enhancement tools featured in visionCATS exploit the full potential of the TLC Visualizer 2. Images are automati-cally captured based on an optimized control of the illumination and parameters specified in the TLC/HPTLC method. Sophistica-ted algorithms guarantee the highest image quality for identifi-cation of even the weakest zones.

HDRI is a technique used to reproduce a greater dynamic range of luminance levels. Multiple standard-dynamic range images are taken at different exposures and merged into a single HDR image. For HDR images under UV 366 nm a set of dedicated en-hancement tools is available. “Exposure Normalization” allows the retreatment of the image by normalizing the exposure: the gain is adjusted to an equivalent of 85% detector response ba-sed on a selected area (reference track by default). The tool is used to allow visual comparison of images from different pla-tes with virtually the same exposure settings. The “Clarify“ tool virtually changes the illumination setting after capturing and makes very faint zones visible on an unchanged background.

Localization of even the smallest fractions on the plate can be achieved with the “Spot Amp” tool which allows increas- ing or decreasing the contrast of the image. With “Clean Plate Correction“ an image taken of a neat plate prior to sample application can be subtracted from the image of the developed plate. Thus irregularities of the TLC/HPTLC plate, particularly the structure of the fluorescence indicator, or small variations in layer thickness seen in transmission mode, are efficiently eliminated. The “Clean Plate Correction” is significantly improved due to the increased resolution of the new CCD camera in the TLC Visualizer 2.

“Exposure Normalization” for visualization of weak zones (right) and original image (left)

“Clean Plate Correction”: the corrected image (right) is of superb quality. Due to reduced noise, weaker zones become detectable.

Comparison Viewer

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The “Comparison Viewer” allows easy side by side comparison of tracks from different TLC/HPTLC plates and different detection modes on the same screen:

from different plates

same and/or different plates and/or detection modes

to the “Comparison Viewer”, e.g. plate state (clean, developed, derivatized), wavelengths, RF value, and display settings

track information and reference details

to a different visionCATS installation

“Comparison Viewer”: creation of virtual plates from tracks originating from different plates and detection modes

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3D-image profile

Image-based evaluation

Technical specifications Dimensions and weightWidth: 480 mm, depth: 537 mm, height: 596 mm Net weight: 17.0 kgObject size supportedWith 12 mm lens: up to 20 x 20 cm With 16 mm lens: up to 20 x 10 cmLight sources2 x UV tube short wavelength (254 nm) – direct light 2 x UV tube long wavelength (366 nm) – direct light 2 x white light tube – direct light 2 x white light tube – transmitted light

Camera typeDigital CCD camera, sensor “SONY Super HAD CCD“, HDR

Camera exposure time2.3 ms to 10 s, up to 60 s for HDRI, min. step size 1 µs

ConnectionUSB 3.0 and RS232

Mains voltage100 – 240 V, 50 / 60 Hz, 50 W

Software for instrument controlvisionCATS (2.3 or higher) on MS Windows 7 and Windows 10

visionCATS software enables an image-based evaluation of chromatograms obtained with the CAMAG TLC Visualizer 2.

Conversion of images of TLC/HPTLC chromatograms into analogue curves based on absorption or fluorescence of separated zonesIntegration of chromatograms resulting in semi-quantitative peak data (area and/or height)Semi-quantitative evaluation of samples in comparison to calibration standards on the same plate

Profile ComparisonDisplay of individual chromatogramsPosition of peaks (RF value / migration distance) can be determinedComparison of chromatograms from the same or different plate / image

EvaluationIntuitive data managementSeveral calibration modes (e.g. single level, multi-level, related substances)Re-evaluation of data at any time

Substance assignment

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