Caffeine and Niacin Levels in Energy Drinks By: Liz Knisley & Kelley Smith.
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Transcript of Caffeine and Niacin Levels in Energy Drinks By: Liz Knisley & Kelley Smith.
Caffeine and Niacin Levels in Energy DrinksBy: Liz Knisley & Kelley Smith
Background Information Caffeine toxicity results when a person takes in
250-500 mg of caffeine Symptoms: irritability, nervousness, tremors, and
insomnia Caffeine can become lethal when 1000 mg or
more is consumed Symptoms: seizures or aspiration
Detrimental effect on epilepsy control Pregnant women consuming 6 or more cups a
day can result in miscarriage or low fetal birth weight
Background Information Niacin is a lipid-altering drug
Mainly known to decrease cholesterol levels
Main side effect of niacin is flushing of the skin
If 80 mg is consumed at one period of time it increases the risk of muscle damage Supplements are usually extended
release
Purpose and Method
Purpose: to determine whether the amount of caffeine and niacin in a specific energy drink was dangerous to the human body and in what amount.
Method: use the HPLC to test different contents of energy drinks and compare them to standards run of both caffeine and niacin
What We Did… Ran standards of caffeine and niacin on
HPLC Ran diluted energy drinks to gain spectra
that could be compared to the standards Monster (16 fl. oz.) Red Bull (8.4 fl. oz.) 5 Hour Energy (1.93 fl. oz.) Mt. Dew Kickstart (16 fl. oz.) Rock Star: energy x2 (16 fl. oz.)
HPLC Caffeine Standards
50 ppm, 40 ppm, 30 ppm, 20 ppm, and 10 ppm
Niacin Standards 50 ppm, 40 ppm, 30 ppm, 20 ppm, and 10
ppm Energy drinks were all diluted to 1:8
Errors Ran standards originally to compare
caffeine to previous ran standards Instrument was set to 254 nm
Niacin was not actually 250 mg like it said on bottle Had to reweigh out the niacin for the
stock solution
Set Backs Originally wanted to make a 1:2 dilution
of each energy drink Spectra did not come close to the
standards Tried to dilute to 1:4 and still was off the
chart Finally a dilution of 1:8 worked
Originally wanted to use taurine but the lab did not carry it
Conclusion When wanting more for your money Monster and
Rockstar (energy x2) are the way to go Mt. Dew Kickstart comes in second Red Bull comes in third 5 hour energy is last
You would have to consume 60.452 Monsters or Rockstar at a consecutive times to die from caffeine intake 70.517 Mt. Dew Kickstarts 115.141 Red Bulls 501.002 5 Hour Energys
Future Work Test more energy drinks such as NOS
that contain warning labels for levels of caffeine
Run less diluted standards of niacin so that we can compare the results
Run other compounds associated with energy drinks such as taurine
CitationsAlpert, P. The Health Lowdown on Caffeine. Home Health Care Management Practice. [Online] 2012, 24, 156. http://hhc.sagepub.com/content/24/3/156.full.pdf+html (accessed May 09, 2013).Hochholzer, W., Berg, D., Giugliano, R. The Facts
Behind Niacin. Ther Adv Cardiovasc Dis. [Online] 2011, 5, 227. http://tak.sagepub.com/content/5/5/227.full.pdf+html (accessed May 09, 2013).