By: Scott Karas. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results ...
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![Page 1: By: Scott Karas. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses.](https://reader036.fdocuments.net/reader036/viewer/2022081516/56649d9f5503460f94a897af/html5/thumbnails/1.jpg)
The Treatment and Prevention of Dental
CariesBy: Scott Karas
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Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses
Overview
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What are they?◦ Cavities
How are they formed?◦ Streptococci mutans
How to prevent them?◦ Brush your teeth◦ Vaccine?
Dental Caries
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GTF- Glucosiltransferase GbpB- Glucan binding protein B CAT- Catalytic Domain
◦ GTF epitope GLU- Glucan binding domain SYI- peptide complex
◦ GbpB epitope WIS-Water insoluble glucan
Key Enzymes/Antibodies
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Purpose of this experiment Bacteria used
◦ Streptococci mutans◦ Streptococci sobrinus
Synthetic peptides Three experiments
◦ 24-37 day old rats Measured the levels of antibodies Measured dental caries
Experiment Overview
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Peptides◦ Three monoepitopic◦ Two diepitopic
Proteins◦ GbpB from S. mutans◦ GTP from S. sobrinus
ELISA◦ Measured IgA and IgG antibody levels
Methods
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Experiment 1◦ 40 female rats◦ 6 groups of 7◦ Injected subconsciously
Experiment 2- S. mutans◦ 48 female rats◦ 4 groups of 12 in a cage◦ Injected subconsciously◦ Asphyxiated
Cleaned with beetles and ethanol
Methods
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Experiment 3- S. sobrinus◦ Identical to Experiment 2◦ Streptomycin-resistant S. sobrinus
Bacterial Recovery◦ ELISA◦ Cotton swab
Carie assessment
Methods
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Human Trials◦ Put in cows milk or eggs
Treatment for children or infants◦ “Window of Infectivity”
Treat young mothers◦ Breast feeding
Potential Uses
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Dental Caries Methods Results Potential Uses
Summary
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Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses
Summary
![Page 18: By: Scott Karas. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses.](https://reader036.fdocuments.net/reader036/viewer/2022081516/56649d9f5503460f94a897af/html5/thumbnails/18.jpg)
Smith, Daniel J., William F. King, Joy Rivero, and Martin A. Taubman. "Immunological and Protective Effects of Diepitopic Subunit Dental Caries Vaccines." Infection and Immunity 73.5 (2005): 2797-804. Print.
Russell, Michael W., Noel K. Childers, Suzanne M. Michalek, Daniel J. Smith, and Martin A. Taubman. "A Caries Vaccine?" ProQuest Nursing & Allied Health Source 38.3 (2004): 230-35. Print.
References