By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A...
-
Upload
dylan-murphy -
Category
Documents
-
view
222 -
download
0
Transcript of By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A...
![Page 1: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/1.jpg)
UNCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN
![Page 2: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/2.jpg)
UNCOMMON AMINO ACIDS
Derived from common amino acids
Produced by the parent amino acid being modified after protein is synthesized
Process is called post-translational modification
![Page 3: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/3.jpg)
HYDROXYPROLINE & HYDROXYLYSINE
Both examples of uncommon amino acids
Unlike their parent amino acids, both have hydroxyl groups on their side chains
Found in some proteins in connective tissues
EX: Found in collagen
![Page 4: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/4.jpg)
AMINO ACIDS FORMING PROTEINS
This reaction takes place in the cells by a mechanism
Producing an amide.
Any 2 amino acids can be linked together to form dipeptides
The two amino acids are joined together by a peptide bond Adding another would make it a tripeptide.
Every dipeptide still has a –COO and a –NH3
Amino acids can be linked in different ways to form different dipeptides.
Some protein molecules contain more than 10,000 amino acid units
![Page 5: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/5.jpg)
FORMING PROTEINS
Shorter chains are referred to as peptides while longer chains are referred to as polypeptides
Polypeptides contains a minimum of 30-50 amino acids
Residues are a chain of amino acids
Abbreviations of 1-3 letters are usually used to represent proteins and peptides
![Page 6: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/6.jpg)
SYNTHESIZING
C-terminus- amino acid at the end of a peptide that has a free α-carboxyl group
N-terminus- amino acid at the end of a peptide that has a free α-amino group
Proteins synthesize from the N-terminus to the C-terminus
![Page 7: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/7.jpg)
PROTEINS PROPERTIES
Continuing patterns of peptide bonds form the backbone of peptides and proteins
Side chains- are called an R group
Up to 20 different side chains of the amino acid supply variety and also determine the chemical and physical properties of the protein
The most important property is acid-base behavior
Amino acids behave like zwitterions as do proteins
![Page 8: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/8.jpg)
MORE PROPERTIES
In proteins repulsive forces between like charges on their surfaces determine water solubility
When a protein is at a pH where there is an equal number of positive and negative charges it is at its isoelectric point
When proteins are at their isoelectric point they are least soluble in water and can be precipitated from the solution
![Page 9: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/9.jpg)
PRIMARY STRUCTURE
In a primary structure of a protein the sequence of amino acids form a chain
Most peptides and protein molecules have different sequences of amino acids
Each sequence allows the protein to perform its function
Only a small fraction of all possible protein molecules have been made by biological organisms
![Page 10: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/10.jpg)
PRIMARY STRUCTURE
Like the naming of peptides the assignment of positions of the amino acids starts at the N-terminal end
There are 20 possibilities for the N-terminal amino acid
400 different dipeptides can be formed from 20 amino acids
The primary structure of a protein to a large extent, determines the native, or most frequently reoccurring secondary and tertiary structures
![Page 11: By: Kim & Caitlin U NCOMMON AMINO ACIDS, AMINO ACIDS FORMING PROTEINS, & PRIMARY STRUCTURE OF A PROTEIN.](https://reader035.fdocuments.net/reader035/viewer/2022081514/56649da75503460f94a92f3c/html5/thumbnails/11.jpg)
SEQUENCING
A change in sequence may or may not affect the function of a protein depending upon the change
An example of this is how bovine insulin is used for instant injections for humans. Even though the insulin is not identical in structure to human insulin it still performs the same task when introduced into a human. Although it does perform the same task it is not as effective.
An example of where a small change can have a huge effect is in the protein of the blood, hemoglobin. A change in one amino acid can cause the disease sickle cell anemia