BSC 197: Molecular and Cellular Basis of Life Lab
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Transcript of BSC 197: Molecular and Cellular Basis of Life Lab
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BSC 197: Molecular and Cellular Basis of Life Lab
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TA info
• Drinie Clairardin
• Office/Lab: SLB 245/247
• http://biology197.wikispaces.com/
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Lab Info
• Lab schedule can be found on the syllabus– Course website: www.bio.ilstu.edu/bsc197
• Lab will consist of an exercise one day and a wet lab the following day
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Lab Safety
• Safety is the number one priority
• ABSOLUTELY NO food, drink or tobacco in Lab
• Lab safety equipment available and will be used as needed (eg. Goggles, gloves)
• Location of Eyewash, fire extinguisher
• Do not endanger yourself or others
• Respect everyone in lab
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Assignments• All lab reports must be typed (including graphs)• Do the lab report as follows
– Intro: Short background about the lab; include hypothesis
– Materials and Methods: Summarize briefly; focus on the reasons, don’t need exact details
– Results: tables/graphs of data and text to summarize what they show
– Discussion: analysis of results; troubleshoot lab if it didn’t work
– Conclusion: short summary of everything
• All lab reports are due at the beginning of the lab, two days after the lab was completed
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Plagiarism Policy
• Plagiarism will not be tolerated – Consequences of being caught for plagiarism
include receiving a zero for the class and being referred to student affairs for disciplinary action
• Do your own work– Make your own graphs– Write your own papers– Cite sources
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Exercise 1
• We will use several types of graphs in the lab– Bar (listed as column graph in excel): good
choice when only using a few data points; can have words in the x-axis; x-axis is not evenly numbered
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Graphs (cont’d)
• Line Graph: used when you are looking at a change over time; x-axis not drawn to scale and x-axis can have letters
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Graphs (cont’d)
• Scatter (XY Scatter): X-axis drawn to scale; data will be plotted in its exact position not just at the labeled point; can not be used if there are letters needed on the x-axis
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Graphs (cont’d)
• Pie chart: Used when describing a part or percentage of a whole
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Graphs (cont’d)
• Graph requirements:– X and Y axis titles, labels and units– Graph title (must be descriptive)– Legend
– Failure to include ALL requirements will result in point deductions
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Statistical Calculations
• Pages 11-14 (top portion-not including T-Test)
• Mean- Average
N
XXXXXMEAN
...54321
•N = total number of replicates•X1, X2, X3… Individual data Points
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Mean Calculation Example
• X1 = 79
• X2 = 85
• X3 = 92
• X4 = 87
• X5 = 93
• X6 = 99• (79 + 85 + 92 + 87 + 93 + 99)=535 • 535/6=89.17
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Statistical Calculations (cont’d)
• Standard Deviation (S.D.)- measures variability of the mean
)1(
)()(*..
22
NN
XXNDS
•N- number of replicates•(X2)- Sum of the squares of X•(X)2- The square of the sum of the values for X
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Standard Deviation (S.D.) Example Calculation
Larva # X
1 0.124
2 0.11
3 0.131
4 0.099
5 0.119
Larva # X X2
1 0.124 0.015376
2 0.11 0.0121
3 0.131 0.017161
4 0.099 0.009801
5 0.119 0.014161
Square X values
N = 5
Sum of X = 0.583
Sum of X2 = 0.068599 )15(5
)((0.583)-0.068599)(5..
2
DS
S.D.= 0.012461942
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Statistical Calculations (cont’d)
• Standard Error (S.E.): estimates reliability of the mean
)(
.).(..
N
DSES
5
42)(0.0124619.. ES
S.E.= 0.005573149 = 0.006 (rounded off)
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Statistical Calculations (cont’d)
• Do not round values until the end
• Show all your work for the calculations
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For tomorrow:
• Exercise 1 due
• Lab 1: The Properties of Enzymes – Read Lab 1 in the manual
• Dress appropriately – No open toed shoes or shorts above the
knee!