Automated classification of multiphoton microscopy images ...
Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute...
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Transcript of Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute...
![Page 1: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/1.jpg)
Breast Cancer ECM Visualization using Multiphoton Imaging
Jeremy BredfeldtLOCI, Morgridge Institute for
Research
![Page 2: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/2.jpg)
H&E and SHG
• Protocol– Thaw & fix sample– Section sample to 200 microns on a vibratome– Image with SHG– Paraffin embed tissue wafer– Section and stain for H&E– Slide scan
• Green = SHG signal (Collagen Type I)
![Page 3: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/3.jpg)
Normal Associated Tissue
11.6 mm
H&E
SHG
![Page 4: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/4.jpg)
Invasive Ductal Carcinoma
H&E
SHG
![Page 5: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/5.jpg)
Fibroadenoma
H&E SHG
![Page 6: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/6.jpg)
7.5 mm
Mucinous Carcinoma
H&ESHG
![Page 7: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/7.jpg)
Intrinsic Fluorescence
• Green = SHG• Red = Autofluorescence from FAD or Collagen
type III
![Page 8: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/8.jpg)
FibroAdenoma
![Page 9: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/9.jpg)
![Page 10: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/10.jpg)
Extrinsic Fluorescence
• Green = SHG (Collagen type I)• Red = E-Cadherin stained cells &
Autofluorescence from Collagen type III
![Page 11: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/11.jpg)
Terminal Ductal Lobular Unit (Red) Surrounded by Collagen (Green)
![Page 12: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/12.jpg)
Duct in Cross Section, Surrounded by IDC cells
IDC
Duct
![Page 13: Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.](https://reader035.fdocuments.net/reader035/viewer/2022062313/56649cf95503460f949cb336/html5/thumbnails/13.jpg)
IDC Cells Near a Vessel with ECM
IDC Cells
Vessel