BR-8134A P/ACETM DNA SYSTEM - Bid on Equipmentbidonequipment.info/s/BECKMAN INSTRUMENTS, INC. PACE...

BR-8134A

P/ACETM DNA SYSTEM Flexible, Automated DNA Analysis

BECKMAN

•

A CLEAR ADVANTAGE

FOR MUTATION ANALYSIS,

POLYMORPHISMS,

SYNTHETIC OLIGONUCLEOTIDES,

DNA QUANTITATION,

AND PROTEIN/DNA

INTERACTION

Packaging — Processing

Bid on Equipment 1-847-683-7720

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I HE P/ACE DNA SYSTEM COMBINES TO 61.

THE VISIBLE BENEFITS OF CE FOR DNA ANALYSIS

Just as DNA is not complete without all

four nucleotides, a total capillary electro-phoresis (CE) solution for your application

requires the right mix of hardware, soft-

ware, chemistry and support. The P/ACE

DNA System combines the power of the

P/ACE 5000 capillary electrophoresis

system, LIF (laser-induced fluorescence) detection, P/ACE software, and a broad

range of eCAP'" chemistries with the most

experienced CE support team in the world. The result: automated DNA analysis with

a clarity that is unmatched by traditional

gel electrophoresis methods.

AUTOMATION With the need to do more with less, the P/ACE DNA System

easily automates the DNA analysis

process from gel preparation to data han-

dling. You will save time and money while obtaining better reproducibility and more

information than with slab gels. The

P/ACE 5000's automated sample handling

capabilities eliminate the need for manual

sample loading and gel preparation.

And with on-capillary detection,

quantitation is automatic. There is no time

wasted staining, reading gels with a scanner

or disposing of hazardous materials.

With Beckman's seven years of CE DNA separation experience you spend less

time working out instrument and method

conditions and more time understanding your DNA.

EPRESENTATIVE APPLICATION AREAS OF THE P/ACE DNA SYSTEM

CATEGORY APPLICATION REFERENCE

SIZE POLYMORPHISMS VNTR

STR (microsatellites)

RFLP

RAPD

Beckman Primer 607397

Ulfelder, Kathi and Christian Oste, Sixth Annual Frederick Conference on Capillary Electrophoresis, Session 8 (1995)

'Ulfelder, Kathi et al., Analytical Biochemistry 200 (1992): 260-267

*Tattersall, David et al., Australian Cooperative Research Centre for Viticulture, Program 1, Subprogram 1.1

DNA QUANTITATION Viral Load Determination

Competitive RT-PCR' to measure gene expression

Plasmid Analysis

Schartz, Herbert et al., Journal of Capillary Electrophoresis 1 (1994): 36-54

Beckman Application Information Bulletin A-1788A

Courtney, Bernard et al., Analytical Biochemistry 228 (1995): 281-286

MUTATION ANALYSIS SSCP 'Landers, James et al., Seventh International Symposium on High Performance Capillary Electrophoresis. Poster 313 (1995)

SYNTHETIC OLIGONUCLEOTIDES Purity Analysis 'Beckman Bulletin 7912 Beckman Technical Information Bulletin T-1794A

PROTEIN/DNA INTERACTION Mobility Shift Assay Capillary Electrophoresis. Poster TI21 (1993)

Waschke, Hans et al., Fifth International Symposium on High Performance

DNA quantitation, mutation detection, size polymorphism analysis and

separation of synthetic oligonucleotides are quickly performed using the

automated P/ACE DNA System.

' UV detection was used in the listed reference

' The Polymerase Chain Reaction (PCR) is covered by U.S. Patents owned by Hoffman-LaRoche Inc. All trademarks are the property of their respective owners.

The P/ACE DNA System automates

the separation, quantitation, analysis

and documentation of oligonucleotides,

Polymerase Chain Reaction (KR)

products, and restriction fragments.

Beckman's broad range of eCAP

chemistries, combined with the powerful

P/ACE DNA system, automatically

speeds every aspect of DNA analysis.

CAP NA 110-N Kit

Packaging - Processing



YOU A CLEAR ADVANTAGE IN DNA SEPARATIONS Iso .0 n 01.V. 444 * 000000000 b 440 4 0 * 0 404 440*•44i4 3 44 044. 4". 4g4.3.

BECKMAN'S BROAD RANGE OF eCAP CHEMISTRIES, COMBINED WITH THE POWERFUL P/ACE DNA SYSTEM, AUTOMATICALLY SPEEDS EVERY ASPECT OF DNA ANALYSIS

CLARITY Seeing the "big" picture is

one of the main benefits of the P/ACE

DNA System. From fragment analysis to

DNA-Protein Interaction, you will be

able to separate and quantify with con-

tidence. High resolution separations are

possible with the eCAP DNA capillary

and its replaceable, polyacrylamide gel.

Automated replacement minimizes contam-ination and results in excellent run-to-run

reproducibilities. Combining the selectivity

and sensitivity of the LIF (laser-induced

fluorescence) detector with the data

collection and integration capabilities of

the P/ACE software you can be assured

of seeing minor variances, while using

only a few nanoliters of sample.

As shown in the table (below

left) and on the following pages, the wide

variety of separations possible with the

P/ACE DNA System proves that it is the right combination of CE hardware, soft-

ware, chemistry and support for your

DNA application.

MMEDIATE RESULTS IN A VARIETY OF EV EE

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T24 1000 by

200 by T18 T24

T14 T311000 by

200 by

D1580 PCR products

26 28 30 32 34 36 38 40 42 44 46 TIME (minutes)

I10 RFU

D1S80 Allelic ladder

eCAP dsDNA 1000 Kit

Calibration curve for molecular size

determination. Curve was generated

using dsDNA fragments from a

oX 174 DNA/Fir/ell! digest separated

with the eCAP dsDNA 1000 Kit.

2.986Correlalion Coefficient 0991275

Linear

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VNTR analysis of the PCR-amplified

D1580 locus from a heterozygous

individual, compared to the allelic

standard using the eCAP dsDNA 1000

Kit. A 32 by difference between allelic

fragments is demonstrated in the

sample. Standards of 200 and

1000 by are added for sizing the

DNA fragments.

STR analysis of PCR-amplified genetic

loci, THOI, TPDX, and FES/FPS from

three individuals in one family, demon-

strating paternity. Separations using

the eCAP ssDNA 100-R Kit with

EnhanCE interculator and LIF detection

detect differences of 4 by in size.

TH01 TPDXFES/FPS

Mother

Child

Father

J 22 26 30

34

38 TIME (minutes)

IZE POLYMORPHISMS

Size polymorphism analysis is used to

correlate polymorphic sequences of DNA

with a gene of interest. The P/ACE DNA System with the eCAP T" dsDNA 1000

Kit and EnhanCE intercalating dye pro-vides fast runs with high resolution for PCR-amplified restriction fragment length

polymorphism (PCR-RFLP) or random amplified polymorphic DNA PCR (RAPD-PCR) analyses. LIF detection

provides the high sensitivity required for low sample levels.

DNA profiling or typing for paternity testing and forensic applications

can be done with short tandem repeat (STR) or variable number tandem repeat. (VNTR) analyses. Good reproducibility

and software for molecular sizing facilitates

pattern recognition.

14 15 16

18 TIME (minutes)

Sabin 3

primers and primerAimer

w 7 '2)

Ew 5-

ST

C

3

100CF

80TARGET

0 COMPETITOR 60

U N3 cci

LL20 Is

20

30 TIME (minutes)

Intact plasmid

110 RFU

25 21 13 17 TIME (minutes)

EcoRl full digest

3 Kbp inert —

12 Kbp

9 Kbp vector

CE-LIF analysis of DNA products

generated by competitive RNA-PCR

of cytochrome P450-1A1 mRNA in

dioxin-treated HepG2 cells. Target

sequence is 367 bp; competitor is

228 bp; CR = 5-carboxyfluorescein

marker; IS = 328 by internal standard.

Packaging - Processing Bid on Equipment

1-847-683-7720 www.bid-on-equipment.com

L.ATIONS 911404 OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO 0000.011011.03.i44G00074.401.89.04.11.4640071.004.4408 66666666666666666666666666666666666666666666 666666666666666 leo

"Before and after" separation of

DNA fragments derived from the

complete EcoRl digestion of a 12 Kb

plasmid vs. the undigested plasmid

itself using LIF detection.

LIF (laser-induced fluorescence)

detection of the separation of a

53 by RT-PCR product from the RNA

of the Sabin-3 strain of the polio virus

vaccine. A Haelll-digested oX 174

DNA marker, at a concentration of

10 pg/mL, was co-injected with the

PCR product for size verification.

NA QUANTITATION

Polymerase Chain Reaction (PCR) with

the electrophoretic separation of the amplified products provides the ability to detect and quantitate small amounts

of target DNA. The P/ACE DNA System automates the analysis of the PCR prod-

ucts. With the unique co-injection feature, size and/or quantitation standards are

loaded automatically to eliminate the need for pre-mixing with each sample.

Accurate quantitation at low

levels can often be best achieved through competitive PCR techniques such as

competitive RNA-PCR. The P/ACE DNA System provides superior precision

and eliminates the need for off-line

detection and scanning. Applications such as monitoring

plasmid copy number in cell cultures

require quantitation of large DNA frag-

ments up to 20,000 base pairs. The eCAP'" dsDNA 20,000 Kit includes the capillary, gel, and size standards for these applications.

The analysis of large DNA fragments

can also be used for monitoring plasmid

ligation efficiency where the efficiency is maximized by the ultra-low

sample consumption of the P/ACE DNA System.

eCAP dsDNA 20,000 Kit


www.bid-on-equipment.cr,

FLEXIBILITY TO ADAPT TO YOUR CHANGING

23 25

Trityl-off

17 19 21 TIME (minutes)

Tvityl-on

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0.034

0.030

0.026

0.022

48 0.018

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0.002-

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Packaging - P _

Purity analysis of a crude synthetic

100-mer using eCAP ssDNA 100-R Kit

and UV Detector Option.

Separation of a synthetic 17-mer

oligonucleotide with the trityl group

attached to the 5' end, performed

using the eCAP ssDNA 100-R Kit and

the UV Detector Option. Inset shows

high-speed separation of the same

oligonucleotide accomplished using the

7cm portion of the capillary.

Synthetic 21-mer that is fluorescein-

labeled and purified following synthesis

using the eCAP ssDNA 100-R Kit and

the LIF (laser-induced fluorescence)

detector with the 488 Laser Module.

Flexibility means having a system that can

adapt to meet your varied needs in DNA analysis.

UV detection is available for applications such

as quality checking of synthetic oligonucleotides

while additional laser sources such as the 635 Laser Module allow you to choose the most

convenient or best performing fluorescent dye

for your application. To allow the analysis of

small oligonucleotides and nucleic acids, the

P/ACE DNA System is ideal for non-gel

methods such as capillary zone electrophoresis

(CZE) or micellar electrokinetic capillary

chromatography (MECC).

YNTHETIC OLIGONUCLEOTIDES

0 For applications that are tedious or involve

precious, expensive samples, the quality of (—

oligonucleotide primers and probes is critical. The P/ACE DNA system with the eCAP

ssDNA 100-R Kit provides a fully automated,

rapid, and high-resolution system for checking

these oligonucleotides. Labeled oligonucleotides

may be analyzed using LIF detection. UV

detection is available for the analysis of unlabeled

oligonucleotides including antisense phospho-

rothioate oligonucleotides.

eCAP ssDNA 100-R Kit

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SSCP analysis of PCR-amplified rpoB

gene of M. Tuberculosis indicative of

rifampicin susceptibility (UV detection).

Separation of single-base mutant

strands is shown. Data provided

courtesy of Dr. James Landers,

Director of the Clinical CE facility

at the Mayo Clinic.

Binding of EcoRl enzyme to a JOE-

labeled oligonucleotide. Unbound DNA

versus EcoRl complexed with DNA

are compared. Data provided courtesy

of Dr. P. Mickey Williams, Genentech.

LIF detection with 488 laser and

540 emission.

P/ACE DNA System with

optional 635 Laser Module

Capillary electrophoresis is an excellent

tool for DNA analysis, and the application

of this technology continues to grow

rapidly. With Beckman, the world leader

in capillary electrophoresis, you will keep

pace with these continuing developments.

OUTATION ANALYSIS

Methods for detecting mutations in DNA often require unique electrophoretic con-

ditions. One technique, single-strand

conformation polymorphism (SSCP)

analysis, is based on mobility differences

of ssDNA fragments in non-denaturing gels caused by conformational differences due to point mutations.

ROTEIN/DNA INTERACTION

Proteins and their molecular interactions with DNA are important areas of study in

molecular biology. Protein binding is easily monitored through mobility shift assays.

DNA APPLICATION BULLETINS

APPLICATION BULLETINS

A-1748A

Capillary Electrophoresis of dsDNA Fragments with UV and Laser-Induced Fluorescence Detection

A-1774A

Quantitative Capillary Electrophoretic Analysis of PCR Products Using Laser-Induced Fluorescence Detection

A-1788A

Competitive RNA-PCR by Capillary Electrophoresis and Laser-Induced Fluorescence (LIF) Detection for Quantitation of Cellular mRNA

DS-738A

Analysis of Nucleic Acid Derivatives and Analogs by P/ACE Using Capillary Electrophoresis

SR-183

High Sensitivity Analysis of Nucleotides Using Electrokinetic Injection and Sample Stacking with Micellar Electrokinetic Capillary Chromatography (MECC)

T- 1779A Purity Assessment of Two Synthetic Oligonucleotide Preparations by Capillary Gel Electrophoresis with the P/ACE System

OoomegOAINNIMMIMI




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SUPPORT

SOLUTIONS BECKMAN I.S. EN ISO 9001

Ordering Information

P/ACE DNA SYSTEMS

267817 P/ACE DNA System, LIF detection only (120V). Includes P/ACE DNA System with LIF detector, 488 Laser Module, Workstation Software, LIF Calibration/Test Standard, eCAP dsDNA 1000 and 20,000 Kits, EnhanCE Intercalator, and Literature Package.

261844 P/ACE DNA System, LIF detection only (240V)



267818 P/ACE DNA System, LIF and UV detection (120V). Includes P/ACE DNA System with LIF and UV detectors, 488 Laser Module, Workstation Software, LIF Calibration/Test Standard, eCAP dsDNA 1000 and 20,000 Kits, EnhanCE Intercalator, and Literature Package.

267847 P/ACE DNA System, LW and UV detection (240V)

267845 P/ACE DNA System, LIF and UV detection (220V)

261846 P/ACE DNA System, LIF and UV detection (100V)

ACCESSORIES

267848 UV Detector Upgrade for LIF-only Systems. Includes the UV Detector Options, UV Detector Head and Filters. Field service installation is also included.

267839 635 Laser Module. Includes Semiconductor 635 nm Laser, Calibration/Test Standard, 675 nm Emission Filter, Extra Filter Holder.

DNA CHEMISTRY KITS

477410 eCAP dsDNA 1000 Kit. For analysis of double-stranded DNA with linearity from 100 to 1,000 base pairs. Includes coated capillaries (2), replaceable gel, and standards to perform at least 100 runs.

471486 eCAP dsDNA 20,000 Kit. For analysis of double-stranded DNA with linearity from 1,000 to 20,000 base pairs. Includes coated capillaries (2), replaceable gel, and standards to perform at least 100 runs.

471480 eCAP ssDNA 100-R Kit. For analysis of single-stranded DNA with linearity from 10 to 100 bases. Includes coated capillaries (2), replaceable gel, and standards to perform at least 100 runs. UV detection is required to analyze unlabeled and standard oligonucleotides.

477409 EnhanCE Intercalator, 0.5 ml. For use with either eCAP dsDNA 1000 or eCAP dsDNA 20,000 Kits.

T 11 • E

BECKMAN

Beckman Instruments, Inc. • 2500 Harbor Boulevard, Box 3100 • Fullerton, California 92634-3100 Sales: 1-800-742-2345 • Service: 1-800-551-1150 • Internet: http://www.beckman.com • Telex: 678413 • Fax: 1-800-643-4366

Worldwide Offices: Africa, Middle East, Eastern Europe (Switzerland) (41) 22 994 07 07. Australia (61) 2 844 6000. Austria (43) 222 7292164. Canada (800) 387-6799. China (861) 5051241-2. France (33) 1 43 01 70 00. Germany (49) 89-358700. Hong Kong, PRC (852) 814 7431. Italy (39) 2-953921. Japan 3-5352-2825. Mexico (525) 559-1635. Netherlands 0297-230630. Poland (48) 22 408822, 408833. Singapore (65) 339 3633. South Africa (27) 11-805-2014/5. Spain (1) 358-0051. Sweden (8) 445 11 20. Switzerland (41) 22 994 07 07. Taiwan (886) 02 378-3456. U.K. (01494) 441181. U.S.A. 1-800-742-2345.

BI95-2602-PC-20

©1996 Beckman Instruments, Inc. 0 Printed in U.S.A. on recycled paper.

BR-8134A P/ACETM DNA SYSTEM - Bid on Equipmentbidonequipment.info/s/BECKMAN INSTRUMENTS, INC. PACE...

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