BOTSWANA STANDARD BOS 25:2014inmetro.gov.br/barreirastecnicas/pontofocal/textos/regu...BOS 25:2014...

Animal feeding stuffs — Cattle feed — Specification

BOTSWANA STANDARD BOS 25:2014 Third Edition

BO

S 2

5:2

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BOTSWANA BUREAU OF STANDARDS

BOS 25:2014 Third Edition

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Contents

Foreword ............................................................................................................................................................. ii

1 Scope ...................................................................................................................................................... 1

2 Normative references ............................................................................................................................ 1

3 Terms and definitions ........................................................................................................................... 2

4 Requirements ......................................................................................................................................... 3 4.1 Ingredients ............................................................................................................................................. 3 4.2 Urea and non-protein nitrogenous substances ................................................................................. 3 4.3 Whey powder ......................................................................................................................................... 3

5 Growth stimulant and related compounds, antibiotics, anabolic hormones and thyrostatic substances ......................................................................................................................... 3

5.1 General ................................................................................................................................................... 3 5.2 Antibiotics .............................................................................................................................................. 3 5.3 Anabolic hormones and thyrostatic substances ............................................................................... 3

6 Sampling and compliance with the standard ..................................................................................... 6 6.1 Sampling ................................................................................................................................................. 6 6.2 Limits of variation .................................................................................................................................. 7 6.3 Test samples .......................................................................................................................................... 9 6.4 Testing .................................................................................................................................................... 9

7 Packaging ............................................................................................................................................... 9

8 Labelling ................................................................................................................................................. 9

Annex A (informative) Acts and regulations ................................................................................................ 11


ii

Foreword

This Botswana standard was approved by the Technical Advisory Committee of the Standards Council on

2014-05-14.

The Botswana Bureau of Standards (BOBS) was established under the Standards Act No. 16 of 1995 with a

primary responsibility of preparing Botswana Standards.

This standard is a revision of, cancels and replaces BOS 25: 2008, Animal feeding stuffs – Cattle feeds – Specification.

During the preparation of this standard the following organizations were directly represented in the Cereals

and Pulses Technical Committee:

Botswana College of Agriculture Dr JC Moreki (Chairperson)

Botswana Bureau of Standards Mr MNP Bannyaditse (Technical Secretary)

Botswana College of Agriculture Mr D Seabo

Ministry of Agriculture, Mr T Phirinyane Department of Animal Production

Ministry of Agriculture, Mr SU Tjetjoo Department of Animal Production Nutri Feeds Mr J Agenbag Ministry of Agriculture, Mr J Kgosiemang Department of Veterinary Services Ministry of Health, Mrs R Pitinyane-Modise Food Control Unit Botswana Poultry Association Mr D Gilbert Tholo Holdings Pty Ltd Mr W Faber

Published by: Botswana Bureau of Standards Private Bag BO 48 Gaborone, Botswana Telephone: +267 3903200 Fax: +267 3903120 E-mail: [email protected] Website: www.bobstandards.bw ICS 65.120

© Botswana Bureau of Standards, 2014


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Animal feeding stuffs — Cattle feed — Specification

1 Scope

The standard specifies requirements for compounded cattle feeds.

2 Normative references

The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Botswana Bureau of Standards (BOBS) maintains registers of currently valid documents.

AOAC 952.02, Cobalt in animal feed – Colorimetric method.

AOAC 952.14, Mercury in foods – Colorimetric dithizone method.

AOAC 952.24, Microchemical determination of bromine, chlorine or iodine.

AOAC 957.22, Arsenic (Total) in feeds – Colorimetric test

AOAC 982.29, Vitamin D in mixed feeds, premixes and pet foods – Liquid chromatographic method.

AOAC 996.16, Selenium in feeds and premixes.

AOAC 975.08, Fluorine in animal feeds – Distillation method.

AOAC 999.11, Determination of lead, cadmium, copper, iron and zinc in foods – Atomic absorption spectrophotometry.

BOS 9, Prepackaged goods for the ultimate consumer – Labelling, presentation and advertising–General requirements

ISO 5498, Agricultural food products – Determination of crude fibre content – General method.

ISO 5510, Animal feeding stuffs – Determination of available lysine.

ISO 5983, Animal feeding stuffs – Determination of nitrogen content – Kjeldahl method.

ISO 5984, Animal feeding stuffs – Determination of crude ash.

ISO 6490-1, Animal feeding stuffs – Determination of calcium content. Part 1: Trimetric method.

ISO 6491, Animal feeding stuffs – Determination of phosphorus content.

ISO 6496, Animal feeding stuffs – Determination of moisture content.

ISO 6497, Animal feeding stuffs – Sampling.

ISO 6498, Animal feeding stuffs – Preparation of test samples.

ISO 6579, Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Salmonella spp.

ISO 6651, Animal feeding stuffs – Semi quantitative determination of aflatoxin B1–Thin layer chromatography.


2

ISO 6866, Animal feeding stuffs – Determination of free and total gossypol.

ISO 6867, Animal feeding stuffs – Determination of vitamin E content – Method using high performance liquid chromatography.

ISO 6869, Animal feeding stuffs – Determination of the contents of calcium, copper, iron, magnesium, manganese, potassium, sodium and zinc – Method using atomic absorption spectrometry.

ISO 7251, Microbiology – General guidance for the enumeration of presumptive Escherichia coli – Most probable number technique.

ISO 7302, Cereal and cereal products - Determination of total fat content.

ISO 8294, Animal and vegetable fats and oils – Determination of copper, iron and nickel content – Graphite furnace atomic absorption method.

ISO 11212-2, Starch and derived products – Heavy metals content – Part 2: Determination of mercury content by atomic absorption spectrometry.

ISO 14565, Animal feeding stuffs – Determination of vitamin A content – Method using high performance liquid chromatography.

3 Terms and definitions

For the purpose of this standard, the following terms and definitions shall apply:

3.1 approved: means approved by the relevant authority but, in relation to the use of the

Standards Mark, approved by the Botswana Bureau of Standards

3.2 antibiotics: organic substances produced by fungi, bacteria, or algae. They inhibit or destroy

micro-organisms in the animal that are detrimental to its health

3.3 anti-microbial: a substance destructive to or preventing the development of micro-organisms

3.4 fat: water insoluble substances of plant or animal origin, consisting predominantly of glycerol

esters of fatty acids or triglycerides

3.5 crude fat: the expression ‘crude fat’ shall be construed as having the same meaning as the

word ‘fat’

3.6 batch: a number of collection of product units of the same size, type and style which, have

been manufactured, processed or packaged under essentially the same conditions

3.7 lot: an identified quantity of a consignment having characteristics presumed to be uniform

3.8 anti-nutritional: a substance which, when present in pet foods, reduces growth. Examples

are phytate, protease inhibitors (notably soybean trypsin inhibitor) and excessive dietary fibre

3.9 growth stimulant: is a substance that acts by increasing protein deposition without changing

the protein and energy intake

3.10 feed: edible materials consumed by animals that contribute energy and nutrients to their diet

3.11 rancidity: the development of unpleasant flavours in oils and fats as a result of oxidation/caused by

hydrolysis of fat with the liberation of free fatty acids

3.12 growth promoter: a compound that can improve growth rate and feed conversion in healthy

animals on an optimal plane of nutrition.


3

4 Requirements

4.1 Ingredients

All ingredients shall be free from adulterants and foreign material. Products of animal origin shall not be

included in diets of cattle in accordance with relevant regulatory requirements.

4.2 Urea and non-protein nitrogenous substances

No urea or other non-protein nitrogenous substances, with the exception of amino acids, shall be used in

milk substitutes or in calf meal.

4.3 Whey powder

Not more than 15 % by mass of whey powder may be used in the manufacture of milk substitutes.

5 Growth stimulant and related compounds, antibiotics, anabolic hormones and thyrostatic substances

5.1 General

Growth stimulants and related compounds added to feeds shall be used at the levels approved by the

Ministry of Agriculture.

5.2 Antibiotics

Antibiotics approved by the Ministry of Agriculture may be incorporated in cattle feeds, but a statement of

their nature and inclusion levels shall be included on the label.

5.3 Anabolic hormones and thyrostatic substances

The manufacture, importation, usage, handling, storage, transportation, distribution and sale of anabolic

hormones and thyrostatic substances for use in animals is prohibited.

Cattle feeds shall not exceed upper limits on pathogens, toxins and anti-nutritional factors specified in

Table 1.

Table 1—Upper limits on pathogens, toxins and anti-nutritional factors

Pathogens, toxins and anti-

nutritional factors

Class of diets

Test method Dairy cows and

calves

Weaners Bulls All other

cattle

Aflatoxin B1, mg/kg 0.2 0.2 0.2 0.2 ISO 6651

Free Gossypol, mg/kg 120 200 600 600 ISO 6866

Salmonella spp, cfu/ml 0 ISO 6579

E.coli, cfu/ml 0 ISO 7251


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Table 2—Mineral and vitamin maximum tolerable levels for dairy cattle

Nutrient Content Test method

Calcium, g/kg 20 ISO 6490-1

Phosphorus, g/kg 10 ISO 6491

Magnesium, g/kg 5 ISO 6869

Potassium, g/kg 30 ISO 6869

Sulphur, g/kg 4 -

Iron, g/kg 10 ISO 6869

Cobalt, g/kg 10 AOAC 952.02

Manganese, g/kg 10 -

Zinc, g/kg 5 ISO 6869

Iodine, g/kg 0.5 ISO AOAC 952.24

Selenium, g/kg 0.2 AOAC 996.16

Vitamin A, IU/kg 660 000 ISO 14565

Vitamin D, IU/kg 100 000 AOAC 982.29

Vitamin E, IU/kg 20 000 ISO 6867

Table 3—Nutrient requirements for calf feed

Nutrient

(g/kg)

Calf starter Calf grower Test method

Crude protein 180 160 ISO 5983

Moisture, max. 120 120 ISO 6496

Fat, min. 25 25 ISO 7302

Crude fibre, max. 90 90 ISO 5498

Calcium 16 (min) 16 (max) ISO 6490-1

Phosphorus, min. 6 6-30 ISO 6491


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Table 4—Nutrient requirements for beef cattle feed

Nutrient (g/kg) Grower

ration

Beef

finisher

Bull

ration

Maintenance

ration

Test methods

Crude protein 160 120 130 100 ISO 5983

Protein ex NPN, max 120 40 40 40 ISO 5983

Moisture 120 120 120 120 ISO 6496

Fat 25 25 25 15 ISO 7302

Crude Fibre 150 120 150 200 ISO 5498

Calcium 8 10 10 15 ISO 6490-1

Phosphorus 3.5-30 3.5 3.5 3 ISO 6491

Table 5—Nutrient requirement for dairy cow feed in g/kg

Crude

protein

Protein

NPN

Moisture Fat Crude fibre Calcium Phosphorus

min max max min max min max min max min

Complete

feed

Early

lactation

(0-120

days)

150 30 120 25 70 135 250 6 10 3.6

Mid

lactation

(121-240

days)

130 30 120 25 70 135 200 5 10 3

Late

lactation

(241-300

days)

120 30 120 25 70 135 280 5 10 2.8

Concentrated dry cow

feed

110 35 120 25 85 - 120 2.5 8 5

Concentrated dry cow

+ anionic salts (a)

150 35 120 25 85 - 120 - 15 5

Concentrated dairy

feed

120 35 120 25 85 - 120 - 15 5

(a) Dry cow plus anionic salts: maximum intake = 3kg per cow per day at maximum NPN inclusion


6

Table 6—Maximum tolerable dietary levels of undesirable elements

Element Maximum tolerable levels

g/kg

Test method

Aluminium 10 -

Arsenic 10 AOAC 957.22

Bromine 20 AOAC 952.24

Cadmium 0.05 AOAC 999.11

Fluorine 0.4 AOAC 975.08

Lead 0.3 AOAC 999.11

Mercury 0.02 ISO 11212-2, AOAC 952.14

Molybdenum 0.1 -

Nickel 0.5 -

Vanadium 0.5 -

NOTE Where minimum and/or maximum are not specified, then Table 9, Table 10 and Table 11 shall apply.

6 Sampling and compliance with the standard

6.1 Sampling

Sampling shall be done in accordance with Table 7 and Table 8 (ISO 6497).

Table 7—Sampling from packages or other containers

Number of packages /containers in the lot Minimum number of separate bags samples

1 to 16 1

17 to 200 2

201 to 800 3

More than 800 4

Table 8—Sampling from bulk

Mass of the lot (tonnes) Minimum number of separate bulk samples

Up to 1 1

1 to 10 2

More than 10 to 40 3

More than 40 4


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6.2 Limits of variation

Acceptable sampling and analytical variation shall be in accordance with Table 9 and Table 10.

Analytical sampling variations (ASVs) are guidelines for helping inspectors to make routine decisions

on acceptability of products appearing to be marginally acceptable. ASV values are not intended to

allow real deficiencies or excess of the guaranteed ingredient. They are not intended to cover sloppy

work, poor sampling or any deficiency in analytical procedures. They are intended to apply to individual

determinations made under routine conditions on a single sample. A history of seven or eight samples

of a given product each of which is slightly deficient from the guarantee, but none of which is deficient

as much as the analytical variation, is ample justification for the control official to take action.

Table 9 — Acceptable analytical and sampling variations of

moisture, protein, fat, fibre and ash

Registered nutrient level (X),

%

Variation (A) from X Relative variation (RV) from X,

%

1 0.25 25.0

2 0.30 14.9

3 0.34 11.5

4 0.39 9.8

5 0.44 8.8

6 0.48 8.1

7 0.53 7.6

8 0.58 7.2

9 0.63 6.9

10 0.67 6.7

12 0.77 6.4

14 0.86 6.1

16 0.95 6.0

18 1.05 5.8

20 1.14 5.7

25 1.38 5.5

30 1.61 5.4

35 1.84 5.3

40 2.08 5.2

50 2.55 5.1

60 3.02 5.0

70 3.48 5.0

80 3.95 4.9

Note 1: Values in Table 9 can be calculated as follows:

203125.0046875.0 XA 100X

ARV


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Table 10 — Acceptable analytical and sampling variations of calcium, sodium, phosphorus,

chloride, potassium, magnesium, and selenium

Registered nutrient level (X),

%

Variation (A) from X Relative variation (RV) from X,

%

1 0.25 25.0

2 0.30 14.9

3 0.34 11.5

4 0.39 9.8

5 0.44 8.8

6 0.49 8.1

7 0.50 7.2

8 0.52 6.5

9 0.54 6.0

10 0.55 5.6

12 0.59 4.9

14 0.62 4.5

16 0.66 4.1

18 0.69 3.9

20 0.73 3.6

25 0.82 3.3

30 0.90 3.0

35 0.99 2.8

40 1.08 2.7

45 1.16 2.6

50 1.25 2.5

60 1.42 2.4

70 1.60 2.3

80 1.77 2.2

6 5 to 80 % 3810.001738.0 XA 100X

ARV

1 % to <6 % 203125.0046875.0 XA 100X

ARV


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Table 11— Acceptable analytical and sampling variations of micro minerals, vitamins,

medications, micro elements (0-1000 mg/kg) finished feeds and ingredients

Registered level (X)

Variation (A) from X Relative variation (RV) from X

%

% mg/kg

0.10 1000 0.040 40.0

0.25 2500 0.075 30.0

0.50 5000 0.133 26.7

0.75 7500 0.192 25.6

1.00 10 000 0.250 25.0

016667.023333.0 A 100X

ARV

Values <0.10 % (1000mg/kg) relative variation (RV) from X= 50 %.

6.3 Test samples

Using an appropriate sampling instrument, adequate quantities of the product shall be withdrawn from

the selected packages/containers and bulk consignment according to Table 7 and Table 8, respectively.

Preparation of laboratory samples shall be in accordance with ISO 6497.

6.4 Testing

The composite samples shall be tested for all parameters as outlined in Table 1 to Table 6.

7 Packaging

Cattle feeds shall be packaged in new, clean, dry and sealed breathable bags.

8 Labelling

Every container of cattle feed purporting to comply with this standard shall meet the requirements of BOS 9

and additionally bear the following information, which shall be printed either on the container or on the label

attached thereto:

a) the name of the feed;

b) the batch number;

c) country of origin;

d) date of manufacture;

e) best before date;

f) name and address of manufacturer;

g) net mass

h) a description of the type of the cattle feed and an indication of the class of cattle to which it is to be fed;

i) the declared contents of:


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1) crude protein;

2) crude fat;

3) crude fibre;

4) calcium;

5) total phosphorus; and,

6) moisture.

7) if antibiotics are present, a statement of their nature and inclusion levels in accordance with 4.2.3;

8) urea warning.


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Annex A (informative)

Acts and regulations

[1] Fertilizers, farm feeds, agricultural remedies and stock remedies Act, 1947 (Act No. 36 of 1947–Republic of South Africa)

[2] Statutory Instrument No. 126 of 2004–Botswana

[3] Diseases of animals (Prohibition of use of anabolic hormones and thyrostatic substances) Regulations–Botswana

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