BME 130 – Genomes Lecture 4 Sequencing technology II Next generation sequencing.
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BME 130 – Genomes Lecture 4 Sequencing technology II Next generation sequencing
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Transcript of BME 130 – Genomes Lecture 4 Sequencing technology II Next generation sequencing.
454 Process Overview
1) Prepare Adapter Ligated ssDNA Library
2) Clonal Amplification on 28 µ beads
4) Perform Sequencing by synthesison the 454 Instrument of ~250,000 molecules
3) Load beads and enzymes in PicoTiter Plate™