Blood Banking to Bone Banking: One year experienceOne year experience Dr. Ankit Mathur Consultant...
Transcript of Blood Banking to Bone Banking: One year experienceOne year experience Dr. Ankit Mathur Consultant...
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Blood Banking to Bone Banking:
One year experience
Dr. Ankit Mathur
Consultant Transfusion Medicine
Rotary Bangalore ttk Blood Bank
Bangalore Medical Services Trust
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BMST Tissue & Bone Bank
• BMST was established in the
year 1984– Rotary Bangalore –TTK blood
bank, Regional Blood Transfusion Center
– Day care Blood Transfusion center
– HLA Laboratory
– Community Services Department
• BMST Tissue Bank: Bones & amniotic membrane
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Blood Banking to Tissue Banking
• Transfusion services are qualified to provide organ & tissue transplant support
• AABB standards addresses receipt, storage, processing & transportation of tissue allografts
• In UK, tissue services is part of NHS blood & transplant
• India: very few tissue banks & not associated with BTS
• BMST: only Tissue Bank associated with Transfusion & HLA services
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Tissue & Bone Bank
• Facility which provides
– spectrum of fresh or preserved biological tissues
– bones for transplantation
• Tissues must be of predictable clinical function and free from transmissible disease
• Tissues such as bone, cartilage, ligaments, tendons, heart valves, blood vessels, skin and amnion are currently being banked the world over
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Banked bones: Advantages
• Eliminate the risks and the expense of the additional incision necessary for acquiring an autograft
• Avoid the creation of a permanent defect in the tissue recovery site which sometimes could give
rise to major complications
• Large variety and quantity of tissue
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Tissue procurement
• Donated bone can be obtained after surgical procedures like hip or total knee replacement
• Necessary screening
and informed written consent
• Tissue specimen & blood sample along with consent form
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Tissue procurement
• Donor’s blood is tested for HIV/AIDS, Hepatitis B & C and Syphilis
• Tissue stored at -80 deep freezer as “Quarantine”
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Processing
• Only Samples tested negative of infection are processed further
• Wet processing: tissue is cleaned of blood and in the case of bone the soft tissue and bone marrow is removed
• Subjected to heat and chemical treatment and then stored in -80ºC freezers
• Heat: keeping tissue at 60C shaking water bath
• Chemical: with 70% ethyl alcohol
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Dry processing
• Lyophillization
• Finally freeze-dried to remove 95% of their water content
• Looses immunogenesity
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Terminal sterilization
• Double packed in a sterile environment under LAF
• Terminal sterilization by exposing them to
gamma radiation by the dose of 25 KGy
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Bone Allograft
• Shelf life 3 years
• Storage at RT
• No antigenesity
• No tissue typing required
• Risk of rejection: almost NIL
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Range of Bone allografts at BMST
• Freeze dried Femoral head
• Frozen femoral head
• Freeze dried tibial cuts
• Frozen tibial cuts/ slices
• Bone blocks of different sized
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Strategy for risk reduction
• Donor screenings
• Stringent infection testing
• Validated chemical cleaning and disinfection process
• Freeze drying
• Gamma irradiation in final packaging for terminal sterilization
• Traceability
• Quality control testing: Bio burden test & moisture
content analysis
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Use of Bone allograft
• Effective in facilitating the formation of new bone
• serves as a scaffold for new bone (osteoconduction: guiding the reparative growth of the natural bone)
• used to fill cavities in a patient’s bone
• replace deceased or damaged bone
• correct deformities, provide structural support
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BMST Tissue Bank: 2011
• Total Bone collected: 469
• Discard: 52
• Total bone allograft issued: 320
– Frozen 43
– Freeze dried: 277
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Registration
• No government rules -regulations for tissue bank & no licensing
• TB may covered by Transplant of human organ act, 1994
• Some state health dept register TB under this act, some do not.
• BMST Tissue & bone bank: under consideration by Health Dept, Karnataka
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Future of Tissue Banking in India
• NEED….
• Registration/ licensing
• Collection of tissue from cadaver
• Awareness about donation & use of tissue allograft
• Transfusion services should take the challenge across India