BLAST DSAP PARt 2 -...
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![Page 1: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/1.jpg)
DSAP PARt 2:BLAST
![Page 2: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/2.jpg)
Review
![Page 3: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/3.jpg)
What You Know So Far...
![Page 4: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/4.jpg)
Review
![Page 5: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/5.jpg)
BLASTn
![Page 6: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/6.jpg)
Running a Blastn Search
![Page 7: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/7.jpg)
![Page 8: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/8.jpg)
![Page 9: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/9.jpg)
![Page 10: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/10.jpg)
![Page 11: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/11.jpg)
![Page 12: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/12.jpg)
Let’s Go More In-Depth...
1. Eval
Other Things:-Gaps-Mismatches-Length-Graphic Summaries
Is it significant?
![Page 13: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/13.jpg)
Gaps/Mismatches
Amino Acid
“Degenerate Code”
![Page 14: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/14.jpg)
Why?
1. Bad Sequences2. Different Organisms
![Page 15: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/15.jpg)
Length
USELESS!!
![Page 16: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/16.jpg)
Use the Graphic Report
?
![Page 17: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/17.jpg)
Is this a good result?Is this significant?
![Page 18: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/18.jpg)
So, why Blastx??
Blastn:
Blastx:
![Page 19: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/19.jpg)
Blastx
![Page 20: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/20.jpg)
Blastx
![Page 21: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/21.jpg)
Frames
![Page 22: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/22.jpg)
Answer the Q’s
![Page 23: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/23.jpg)
What’s a Reading Frame?
![Page 24: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/24.jpg)
What’s a Reading Frame?
![Page 25: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/25.jpg)
The “ORF”“OPEN READING FRAME”
![Page 26: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/26.jpg)
Toolbox
Copy/Paste DNA
Select Your Reading Frame
![Page 27: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/27.jpg)
Protein Sequence
LONGEST ORF
![Page 28: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/28.jpg)
Finally-Your Sequence
(Check that it matches Blastx)
![Page 29: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/29.jpg)
Answer the Q’s
![Page 30: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/30.jpg)
Blastp!!!
![Page 31: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/31.jpg)
Blastp!!!
![Page 32: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/32.jpg)
Answer the Q’s
![Page 33: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/33.jpg)
Blastp v.s. Blastx
![Page 34: BLAST DSAP PARt 2 - waksman.weebly.comwaksman.weebly.com/uploads/1/2/5/5/12556652/dsap_part_2-_blast... · DSAP PARt 2: BLAST. Review. What You Know So Far... ... WSSP Translator](https://reader031.fdocuments.net/reader031/viewer/2022021901/5b8e402709d3f2c65c8d3b11/html5/thumbnails/34.jpg)
UR DONE WITH BLAST
NOW U CAN GO ANALYZE THE STUFF!!!!!!!!!!!!!!!!!!!! <33333333333