Biotechnology & Genomics. DNA Cloning Producing identical copies through asexual means.

of 27 /27
Biotechnology & Biotechnology & Genomics Genomics

Embed Size (px)

Transcript of Biotechnology & Genomics. DNA Cloning Producing identical copies through asexual means.

  • Biotechnology & Genomics

  • DNA Cloning Producing identical copies through asexual means.

  • Uses:determine genetic differences research purposesex disease mechanism, industrial usesgenetic modification or organisms or gene therapygenetic testing / crime investigation , forensics

  • Recombinant DNA (rDNA) technologyVector + foreign DNAex. PlasmidNecessary to make rDNArestriction enzyme = sticky ends cats specificallyDNA Ligase

    PCR, process that uses DNA polymerase

  • DNA AnalysisDNA fingerprintingCut DNAunique band patternElectrophoresis used to separate DNA by size for analysisRNA , Protein

  • DNA Fingerprinting & Paternity

  • ** uses electrophoresis to move charged molecules through a matrix

  • Biotechnology Products some made by genetic engineering

  • Products of genetics modification proceduresA Transgenic bacteria uses Medicines, hormones, vaccines, agriculture, industry to clean wasteB Transgenic plants Assist agriculture, make productsC Transgenic animalsTransform egg cells larger / more productive animalsD Stem cell research Medicinal uses

  • Transgenic Mammals

  • Genomic Differences BetweenChimps & Humans

  • Genomics study of genesHuman genome project = 25,000 coding genesComparing genomes = much similarity; therefore, possible uniqueness is caused by the regulation of our geneshow our bodies function, how to prevent diseases, what makes different species unique, and even how life evolved on earth.

  • Hap Map projectA HapMap is a catalog common sequence differences that occur in a speciesThe goal of the project is to link haplotypes to risk for specific illnessesMay lead to new methods of preventing, diagnosing, and treating disease

  • Epigenetics DNA packaging causing expression or no expression of genes depends on the environmental factors one is exposed to.

  • Genetic profile the human genotype of a person

    To help disease risk factors.

  • ProteomicsStudy of proteinsStructure & function of proteins to design drugs

  • BioinformaticsUsing computers to study the genome

  • Gene TherapyInserting genetic material into human cells for the treatment of a disorder.

    Ex VivoChildren with Severe Combined ImmunodeficiencyBone Marrow Stem CellsIn VivoCystic FibrosisNasal / Respiratory Spray

  • Gene TherapyHowStuffWorks Videos "Through the Lens: Gene Therapy"HowStuffWorks Videos "The Power of Genes: Gene Therapy"

  • Study QuestionA plasmid Yip5 is cut:Two linear fragments of 942 and 4,599 base pairs (5,541 - 942 = 4,599). EcoRI and EagI Two linear fragments of 2,003 (2,035 - 32) and 3,538 (5,541 - 2,003) base pairs. HindIII and ApaI Three linear fragments of 2,003, 2,881 (4,916 - 2,035), and 657 [5,541 - (2,003 + 2,881)l base pairs. HindIII, ApaI, and PvuI The 942-base-pair fragment & The 4,599-base-pair fragment would be cleaved into two fragments of 2,305 (3,247 - 942) and 2,294 (4,599 - 2,305) giving 3 total fragments. EcoRI and EagI,PvuIIConstruct the plasmid

  • More Study QuestionsPCR makes gene cloning possible because it enables lab technicians to do what very quickly?If a plasmid is cut with a restriction enzyme 3 times how many linear fragments will it produce?How will they be arranged on an electrophoresis gel?

  • But wait, theres more!!Explain what recombinant DNA is:If a specific DNA code is removed from a bacteria and placed into another bacteria what is this process called?Why are restriction enzymes generally used in a lab?Dinosaur DNA has been found in very few fossils, scientists want to compare the dino DNA to DNA of reptiles, what technique will they use to increase the amount of dino DNA?

  • Complete the table

  • The Last One!!Explain how a gene from humans can be cloned and produced in yeast, [what process is similar]