1

An RFLP was found to be associated with the X-linked recessive disorder hemophilia A in a particular family. A pedigree and Southern blot analysis are shown below. Individual II-1 is affected. Assuming that no recombination has occurred, what is the status of the individuals in generation III with respect to hemophilia A?

a. III-1 and III-3 are affected, III-2 is normal, and III-4 is a carrier. b. III-1 and III-3 are affected, III-2 is normal, and III-4 is not a carrier. c. III-1 and III-3 are normal, III-2 is affected, and III-4 is a carrier. d. III-1, III-2, and III-3 are affected, and III-4 is a carrier. e. All individuals in generation III are normal.

2

1. A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene. Which of the following techniques would accomplish this goal? A. Southern blotB. northern blotC. western blotD. eastern blot

2. You are hired as a scientific consultant for a popular television show about forensic analysis of crime scene evidence. You are told that in an upcoming scene, investigators will compare the DNA profiles of a known suspect and an unknown biological sample collected from the victim. You are asked to write up a brief explanation of the primary technique required for discussion in the scene. Which technique should you write about? A. DNA fingerprintingB. DNA footprintingC. RFLP analysisD. PCR

3. Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another, using molecular cloning techniques? A. restriction fragment length polymorphism dataB. sequence analysisC. Southern blot dataD. restriction maps of the plasmids

  4 Which of the following two enzymes are needed to construct a cDNA library? A. reverse transcriptase and DNA ligaseB. reverse

transcriptase and Taq polymeraseC. DNA ligase and Taq polymeraseD. Taq polymerase and RNA polymerase  5. In a manual enzymatic DNA sequencing reaction, the nucleotide ddT is incorporated A. at every T position in the synthesized DNA strand;

the reaction is terminated when all T positions have been filled with ddT.B. randomly at a single T position in the synthesized DNA strand, leading to termination of the reaction.C. at several T positions in the synthesized strand, leading to termination of the reaction.D. randomly at a A position in the template DNA strand, leading to termination of the reaction.