BIOSAFETY MANUAL 2017 - Dalhousie University · PDF file Dalhousie University is committed to...

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  • BIOSAFETY MANUAL

    2017

  • i

    Dalhousie University Biosafety Manual

    All new staff and students are required to read this document prior to beginning any work using biological agents. After reading please date and sign to acknowledge that you have done so.

    Name (please print) Signature Date

  • ii

    Telephone Contact Information

    1. Incidents Involving Biohazardous Materials

    Biological Safety Manager Pam Gallant 902-494-4452 2. Emergency Numbers EMERGENCIES 902-494-4109 Dalhousie Security 902-494-6400 Dalhousie Security (Truro) 902-893-4190 3. Members of the Biosafety Committee (2017) Halifax: Mr. J. Aguinaga 902-494-1241

    Dr. R. Anderson 902-494-1063 Dr. J. Archibald 902-494-2536

    Ms. P. Gallant 902-494-4452 Dr. C. Harvey-Clark 902-494-1270 Dr. T. Hatchette 902-473-6885 Dr. D. Hoskin 902-494-6509

    Dr. R. McLeod 902-494-7013 Mr. G. McNutt 902-494-2755 Dr. D. Mitchell 902-494-2785 Dr. G. Ray 902-494-2359

    Dr. N. Thomas 902-494-8065

    Truro: Ms. S. Langille 902-893-2446

    Ms. E. Osmond 902-893-4190 Dr. S. Yurgel 902-893-6003

    Saint John:

    Dr. A. Adisesh 506-636-6016 Dr. K. Brunt (Chair) 506-636-6006

  • iii

    Table of Contents

    Chapter 1: Introduction .................................................................................................... 1 1.1 Definitions .............................................................................................................. 1

    Chapter 2: Regulatory Agencies ..................................................................................... 3

    2.1 Canadian Food Inspection Agency ........................................................................ 3 2.2 Public Health Agency of Canada ........................................................................... 3

    Chapter 3: Biosafety Program Organization & Administration ......................................... 5

    3.2 Responsibilities ...................................................................................................... 5 3.3 Biosafety Committee .............................................................................................. 6 3.4 Biosafety Training .................................................................................................. 7 3.5 Medical Surveillance Program and Exposure Response ...................................... 7 3.7 Incident Reporting ................................................................................................. 8

    Chapter 4: Biological Material ......................................................................................... 9

    4.1 General Classifications .......................................................................................... 9 4.2 Prions .................................................................................................................... 9 4.3 Recombinant DNA ............................................................................................... 10 4.4 Animals ................................................................................................................ 10 4.5 Viral Vectors ........................................................................................................ 12 4.6 Bloodborne Pathogens (BBP) .............................................................................. 15

    Chapter 5: Risk Groups and Containment Levels ......................................................... 19

    5.1 Risk Factors ......................................................................................................... 19 5.2 Risk Groups ......................................................................................................... 19 5.3 Containment Levels and Containment Zones ...................................................... 23 5.4 Laboratory Acquired Infections ............................................................................ 24

    Chapter 6: Laboratory Biosafety .................................................................................... 25

    6.1 Access/Security Controls ..................................................................................... 25 6.2 Use of Personal Protective Equipment (PPE) ...................................................... 25 6.3 Safe Work Procedures ......................................................................................... 26 6.4 Containment Level 2 Requirements ..................................................................... 28 6.5 Potentially Hazardous Operations and Preventive Measures to Reduce Risks ... 28

    Chapter 7: Biosafety Cabinets ....................................................................................... 34

    7.1 Class I .................................................................................................................. 35 7.2 Class II ................................................................................................................. 37 7.3 Class III ................................................................................................................ 38 7.4 Effective Use of BSCs ......................................................................................... 40

    Chapter 8: Sterilization and Disinfection ........................................................................ 42

    8.1 Autoclaves ........................................................................................................... 43

  • iv

    8.2 Ultraviolet Lamps ................................................................................................. 47 8.3 Chemical Disinfectants ........................................................................................ 49 8.4 Laboratory Spills .................................................................................................. 52

    Chapter 9: Transportation of Biological Agents ............................................................. 54 Appendix 1: Posters ...................................................................................................... 56 Appendix 2: Record Keeping Forms.............................................................................. 62 Appendix 3: Biosafety Policies ...................................................................................... 65

  • 1

    Chapter 1: Introduction Dalhousie University is committed to providing a safe laboratory environment for its faculty, staff, students, and visitors. Dalhousie University’s Biosafety Program and Policies are designed to help foster a safe and healthy environment that adequately supports work and study. The goal of the Biosafety Manual is to minimize the risk of laboratory incidents by providing laboratory personnel with the proper information needed to work safely. The Biosafety Manual also intends to aid in the protection of laboratory personnel and the surrounding environment from possible exposure to biohazardous material. The Biosafety Manual provides information, guidelines, and policies that should be used in conjunction with other resources to safely work with and in the environment of biohazardous materials.

    Workers must also be aware of the complex web of laws and regulations that place special responsibilities on people, specifically:

    Canada: Hazardous Products Act, 1985 Health of Animals Act, 1990 Transportation of Dangerous Goods Act, 2016 Controlled Drugs and Substances Act, 1996 Nuclear Safety and Control Act, 1997 Canadian Environmental Protection Act, 1999 Human Pathogens and Toxins Act, 2009 Human Pathogens and Toxins Regulations, 2015 Nova Scotia: Environment Act, 1994-95 Occupational Health and Safety Act, 1996 Halifax Regional Municipality By-Laws: Solid Waste Collection and Disposal (By-law S-600 as amended), 1999 Wastewater Discharge (By-law W-101), 2001

    1.1 Definitions Biological material – material present or produced in a living organism that contains genetic information and is capable of reproducing or being reproduced in a biological system. This material may pose a risk to health and safety, or the environment. Biohazard – biological material that constitutes a hazard to the health and safety of humans, animals, or the environment.

    http://laws-lois.justice.gc.ca/eng/acts/H-3/ http://laws-lois.justice.gc.ca/eng/acts/h-3.3/ http://www.tc.gc.ca/eng/tdg/clear-tofc-211.htm http://www.tc.gc.ca/eng/tdg/clear-tofc-211.htm http://laws-lois.justice.gc.ca/eng/acts/C-38.8/ http://laws-lois.justice.gc.ca/eng/acts/N-28.3/ https://www.ec.gc.ca/lcpe-cepa/default.asp?lang=En&n=26A03BFA-1 https://www.ec.gc.ca/lcpe-cepa/default.asp?lang=En&n=26A03BFA-1 http://www2.parl.gc.ca/HousePublications/Publication.aspx?Docid=4015133&file=4 http://lois-laws.justice.gc.ca/eng/regulations/SOR-2015-44/ http://lois-laws.justice.gc.ca/eng/regulations/SOR-2015-44/ http://nslegislature.ca/legc/statutes/environment.pdf http://nslegislature.ca/legc/statutes/environment.pdf http://nslegislature.ca/legc/statutes/occupational%20health%20and%20safety.pdf http://nslegislature.ca/legc/statutes/occupational%20health%20and%20safety.pdf http://www.halifax.ca/legislation/bylaws/hrm/bls600.pdf http://www.halifax.ca/legislation/Bylaws/hrm/blw101.PDF

  • 2

    Biosafety – practices, procedures, and uses of equipment to