Biosafety level and biosafety cabinets

Biosafety LEVELS & Biosafety LEVELS & BIOSAFETY Cabinets BIOSAFETY Cabinets

http://www.techef.in/

https://www.facebook.com/Techef-1730204353883315/

Bio safety Levels Air Filtration in Biosafety cabinets Standards Hood , Laminar flow & Bio safety Cabinets Classification –Bio safety Cabinets

TopicsTopics



Biosafety LevelsBiosafety Levels



Bio Safety Level (BSL)-I:Well-characterized agents not known to consistently cause disease in healthy adult

humans, and of minimal potential hazard to laboratory personnel and the Environment.

Standard practices required:

frequent hand washing door that can be kept closed when working limits on access to the lab space when working no eating, drinking, storage of food in laboratory care to minimize splashes and actions that may create aerosols (tiny droplets) decontamination of work surfaces after every usePrimary Safety Barriers- Not Required




Bio Safety Level (BSL)-II:• Suitable for work involving agents of moderate potential hazard to personnel

and the environment.

• Generally associated with human disease

Safety Equipment (Primary barriers):

• Class I or II Bio safety Cabinets or other physical containment devices (eg…. Aerosol tight lid in Centrifuges) are used

• Personnel Protection Equipment: Laboratory coats, gloves, face protection as needed

Facilities (Secondary barriers):

• Provide lockable doors for facilities that house restricted agents

• Locating new laboratories away from public areas.

• Furniture used in laboratory work should be covered with a non-fabric material that can be easily be decontaminated.

• An eye wash station is readily available.




Bio Safety Level (BSL)-III:Applicable where work is done with indigenous or exotic agents which may cause

serious or potentially lethal disease as a result of exposure by the inhalation route.


• Class I or II Bio safety Cabinets

• Personnel Protection Equipment: Laboratory coats; gloves; face protection as needed

• Facilities (Secondary barriers):

• The laboratory has special engineering and design feature . The exhaust air from the laboratory room is discharged to the outdoors

• The ventilation to the laboratory is balanced to provide directional airflow into the room




Bio Safety Level (BSL)-IV:Dangerous/exotic agents which pose high risk of life-threatening disease, aerosol-

transmitted lab infections or related agents with unknown risk of transmission.


All procedures conducted in Class III Bio safety Cabinets

or Class I or II Bio Safety Cabinets in combination with full-body, air-supplied, positive pressure personnel suit.

Facilities (Secondary barriers):

BSL-3 plus:• Separate building or isolated zone• Dedicated supply and exhaust and decontamination systems




Air Filtration in Biosafety cabinetsAir Filtration in Biosafety cabinets



•High efficiency particulate air (HEPA) filters•Ultra low particulate air (ULPA) filters

Air Filtration in Biosafety cabinetsAir Filtration in Biosafety cabinets

Variations in HEPA filter efficiency from 99.95 % to 99.99 % are usually due to manufacturing techniques.

Depending upon its quality, a HEPA filter is able to trap from 9,995 to 9,999 of every 10,000 particles

An ULPA filter is able to trap from 99,995 to 99,999 of every 100,000 particles

ULPA - this is at least 10 times better than HEPA filtration !!!



International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet



Why a standard ?Why a standard ?




International Standards are mainly issued by two Organisations: International Standard Organisation (ISO), based in Geneva (Switzerland),Comitee Europeen de Normalisation (CEN) based in Brussels (Belgium).

These standards replace all other local standards on the same subject.

The aim of the standards is to construct a mechanism to provide manufacturer and user of a common site to specify technical specifications and features.

Both construction and testing of the cabinet are covered




Standards:BS-5726 (U.K.)NF X44-201 (France)DIN 12950 (Germany) Now Replaced by EN 12469

EN 12469

European Standards on Design, Construction & Testing of Bio safety Cabinets

In addition to comply DIN 12980 for safety handling of cytotoxic drugs

NSF-49 (National Sanitation Foundation) In the USA the NSF-49, was one of the first standards on this subject and was the reference point for a lot of manufacturers. NSF is an independent organisation that works as neutral agency to help on the problems that could affect to public health and environment protection.

Was published in 1976.



Difference between Hood and Safety CabinetDifference between Hood and Safety Cabinet

Exhaust of vapors and aerosols from Laboratory to outside

No validatable product or personnel protection

Validatable personnel, product & environmental protection

Filters and blower(s) incorporated

HoodHood Safety CabinetSafety Cabinet



A fume hood is a ventilation device that is designed to limit exposure to hazardous fumes vapors or dust. A fume hood is typically a large piece of equipment enclosing five sides of a work area, the bottom of which is most commonly located at a standing work height.

Two main types exist, ducted and recalculating. The principle is the same for both types: air is drawn in from the front (open) side of the cabinet, and either expelled outside the building or made safe through filtration and fed back into the room.

Fume HoodFume Hood

Fume Hood



Only for Product Protection No Personnel or Environment Protection

Laminar FlowLaminar Flow

A. front openingB. sashC. supply HEPA filterD. blower

Vertical Laminar Flow



Laminar FlowLaminar Flow

A. front openingB. supply grilleC. supply HEPA filterD. supply plenumE. blowerF. Grille

Horizontal Laminar Flow



Classification of Bio Safety CabinetClassification of Bio Safety Cabinet

Biological Safety Cabinet

Class I Class II

Class III

Type A Type B

Type A I

Type A II

Type B I

Type B II



Classification of Biosafety CabinetClassification of Biosafety Cabinet

Class I: Personnel and Environment protection. Product is not protected.

Class II: Personnel, Product and Environment are protected.

Class III: Maximum protection to Personnel, Product and Environment.




A. Front openingB. SashC. Exhaust HEPA

FilterD. Exhaust plenum

Class I Biosafety Cabinets

This is a negative-pressure, ventilated cabinet usually operated with an open front.

All of the air from the cabinet is exhausted through a HEPA filter either into the laboratory or to the outside.

The Class I BSC is designed for general microbiological research with low- and moderate-risk agents.




A. front openingB. sashC. exhaust HEPA filterD. rear plenumE. supply HEPA filterF. blower

Class II Type A-I Biosafety Cabinets

It is designed with inward air flow to protect personnel, Product & Environment

HEPA-filtered downward vertical laminar airflow for product protection, and HEPA-filtered exhaust air for environmental protection




Face Velocity 75 fpm

70% re circulated through HEPA Filter

30% Exhausted Through HEPA Filter

Cannot be used for Radio Nucleotides and Toxic Chemicals

Biosafety Levels 2 & 3

User, Product, & Environment Protection

Class II Type A-I Biosafety Cabinets




Class II Type A-II Biosafety Cabinets

A. front openingB. sashC. exhaust HEPA filterD. supply HEPA filterE. positive pressure plenumF. negative pressure plenum

Note: The cabinet exhaust needs to be connected to the building exhaust system.




Class II Type A-II Biosafety Cabinets

Downward Velocity 100 fpm

70% re circulated through HEPA

Filter30% Exhausted Through HEPA Filter

Can be used for Radio Nucleotides and Toxic Chemicals

Biosafety Levels 2 & 3User, Product, & Environment Protection

Under Negative Pressure to room

Exhaust Air is Hard Ducted




Class II Type B-I Biosafety Cabinets

A. front openingB. SashC. exhaust HEPA filterD. supply plenumE. supply HEPA filterF. BlowerG. negative pressure exhaust plenum Note: The cabinet exhaust needs to be

connected to the building exhaust system.




Class II Type B-I Biosafety Cabinets


30% re circulated through HEPA Filter

70% Exhausted Through HEPA Filter

Can be used for Radio Nucleotides and Toxic Chemicals (Low Levels / Volatility)





Class II Type B-II Biosafety Cabinets

A. front openingB. SashC. exhaust HEPA filterD. supply HEPA filterE. negative pressure exhaust

plenumF. supply blower G. filter screen

Note: The carbon filter in the building exhaust is not shown. The cabinet exhaust needs to be connected to the building exhaust system




Class II Type B-II Biosafety Cabinets


No Re-circulation100% Exhausted Through HEPA Filter

Can be used for Radio Nucleotides and Toxic Chemicals


Exhaust HEPA Filtered

Exhaust Hard Ducted out.




Class III Biosafety Cabinets

A. glove ports with O-ring for attaching arm-length gloves to cabinet

B. SashC. exhaust HEPA filterD. supply HEPA filterE. double-ended autoclave or pass-

through box

Note: A chemical dunk tank may be installed which would be located beneath the work surface of the BSC with access from above. The cabinet exhaust needs to be connected to the building exhaust system.




Class III Biosafety Cabinets

This is a totally enclosed, ventilated cabinet

Gas-tight construction

Offers the highest degree of personnel and environmental protection from infectious aerosols as well as protection of research materials from microbiological contaminants.



Cytotoxic Drug Safety CabinetsCytotoxic Drug Safety Cabinets

Hepa filter exhaust #2

Hepa filter (pre-filter) #1

Laminar flow filter

Carbon filter exhaust #3 (option)Dedicated exhaust flow blower

All contaminated areas underNEGATIVE pressure

Dedicated Laminar flow blower

70% re circulated HEPA filtered laminar flow Provides sample protection

30% inflow provides operatorSafety barrier



Can you see the problems ?Can you see the problems ?

Window

Air vent

Ceiling clearanceor wall clearance

Front Venting ExhaustBEWARE



Location In LabLocation In Lab

Location A, E and F are well selected.As well influencing

air flows through the Laboratory

personnel traffic is avoided.

Location B is often influenced from air flows in the Room.

Location C is influenced from personnel traffic

and Room air flows.

Location D is also very often influenced from personnel traffic and air flows.

Safety Cabinets should only be placed in quiet areas!

Only validated tests can show placement in the

laboratory is correct.

In this instance the tests are invaluable!



THANK YOUTHANK YOU



Biosafety level and biosafety cabinets

Education

Transcript of Biosafety level and biosafety cabinets