Biophysics Division

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Biophysics DivisionBiophysics DivisionBiophysics DivisionBiophysics Division

Cellular/Molecular Imaging for Cellular/Molecular Imaging for Bonghan System StudyBonghan System Study

2006. 9. 27.2006. 9. 27.Kwang-Sup Soh Kwang-Sup Soh

Biomedical Physics Lab. for Korean MedicineBiomedical Physics Lab. for Korean MedicineSchool of Physics, Seoul National UniversitySchool of Physics, Seoul National University

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Biophysics DivisionBiophysics Division

ContentsContents

I. Introduction on Bonghan SystemI. Introduction on Bonghan System

II. Co-operative Research ProgressII. Co-operative Research Progress

(1)(1) Electron MicroscopeElectron Microscope

(2)(2) Atomic Force MicroscopeAtomic Force Microscope

(3)(3) NanoparticlesNanoparticles


I. Bonghan SystemI. Bonghan System

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Bonghan SystemBonghan System

Organ-Surface BH duct Organ-Surface BH duct (on the surface of organs)(on the surface of organs)

Superficial BH ductSuperficial BH duct(Acupuncture meridian)(Acupuncture meridian)

Superficial BH CorpuscleSuperficial BH Corpuscle(Acupoints)(Acupoints)

skin : superficial BH ductsskin : superficial BH ducts

network network surface of organs : OSBHDsurface of organs : OSBHD

inside vessels : intra-vascular inside vessels : intra-vascular BHD BHD

Intravascular BH Intravascular BH ductduct

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Physiological SignificancePhysiological Significance

New Circulation System New Circulation System Hyaluronic acid, Amino acid, Mono nucleotide, Hyaluronic acid, Amino acid, Mono nucleotide, Hormone: Adrenalin/noradrenalinHormone: Adrenalin/noradrenalin

Bonghan Granules (Bonghan Granules (ФФ≈0.8~2.4≈0.8~2.4μμm) containing DNAm) containing DNA Adult stem cells: cell therapyAdult stem cells: cell therapy Natural “micro-cell”: gene therapyNatural “micro-cell”: gene therapy


II. Research ProgressII. Research Progress

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Future ProspectsFuture Prospects

AnatomyAnatomy

PhysiologyPhysiology

Electron MicroscopesElectron Microscopes

AFMAFM

NanobiotechnologyNanobiotechnology

Optical TweezerOptical Tweezer

2005 Sept, CKC Symposium 2005 Sept, CKC Symposium

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Microscopy Research & Technology, 2006Microscopy Research & Technology, 2006

Electron Microscopic Study of Novel Threadlike Structures on the Surfaces of Mammalian Organs

Byung-Cheon Lee,1 Jung Sun Yoo,1 Vyacheslav Ogay,1 Ki Woo Kim, 2 Harald Dobberstein, 3 Kwang-Sup Soh, 1, † and Byung-Soo Chang 4, †

1Biomedical Physics Laboratory, FPRD, School of Physics and Astronomy, Seoul National University, Seoul, Korea

2National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea

3Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK

4Department of Cosmetology, Hanseo University, Seosan, Korea

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Fig.1. Stereomicroscopic Images of novel threadlike structures

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SEMSEM

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TEMTEM

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Cryo-SEMCryo-SEM FIB/SEMFIB/SEM((CavendishCavendish))

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Atomic Force MicroscopeAtomic Force Microscope

Investigation of morphology and physical properties of Bonghan microcell using AFM and TEM

1Ku Youn Baik, 1Joonhyung Kwon, 1Byung –Cheon Lee, 2Harald Dobberstein, and 1Kwang-Sup Soh$

1Biomedical Physics Laboratory, Department of Physics, Seoul National University, Seoul, 151-747, Korea

2Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK

- 2006.7.2-7 Molecular Cell Biology GRC, NH, USA

Microcell-like granules in the Bonghan corpuscle on the surfaces of mammalian internal organs

1Ku Youn Baik, 1Vyacheslav Ogay, 2Harald Dobberstein, and 1Kwang-Sup Soh

1Biomedical Physics Laboratory, Department of Physics, Seoul National University, Seoul, 151-747, Korea2Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK

- 2006.8.27 WC2006 Conference, Seoul, Korea

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Atomic Force MicroscopeAtomic Force Microscope

Bonghan microcell cycle (Bonghan Kim, 1965)

(1) All the morphological components of organism are ceaselessly reproduced.

(2) The self-reproduction of organism takes the form of Bonghan microcell-cell cycles.

(3) The self-reproduction of organism is performed by the acupuncture-meridian system.

Theory of Bonghan Microcell

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A Topography, B Error, C Magnified of B, D line profile

1.4 um x 1um x 350nm sized oval Bonghan microcell. It shows clearly unique surface structure of layers and portions (B & C) whose size is from 0.2um to 0.6 um in diameter. The line profile analysis shows that the common patterns on the surface are steps of about 20nm height. This pattern is thought to be revealing the stereotype of its membrane structure.

AFM image of Bonghan microcellAFM image of Bonghan microcell

20nm

AA BB CC

DD

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NanobiotechnologyNanobiotechnology

Flowing Channel of NanoparticlesFlowing Channel of Nanoparticles

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