Biomedical Imaging Hyperspectral Imaging
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Northeastern University
Why Hyperspectral
South Bay of California; 101 curves down on the left.
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–2
Why Hyperspectral
Where are the Shrimp?
Hardware
– x, y on camera
∗ Slit, Grating and 2D Camera
· x, λ on camera
· y with time (pushbroom)
Applications
• Law Enforcement (Which crop is illegal?)
• Environmental (e.g. Deep Horizon)
rophores)
Forward Models
• Monte–Carlo
• Fluorescence
Some Common Absorbers
,Wavelength, nm
10 -4
10 -3
10 -2
10 -1
10 0
10 1
10 2
10 3
10 4
Some Common Fluorophores
, Wavelength, nm
488 514 633
Matrix Methods
• Forward Problem
Y = MX
• Typical Example
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–9
Variance, Covariance
• Covariance:
Y =
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–10
Variance, Covariance, Principal Components
• Arrange in Eigenvalue Order Decending
• Remember Y = MX for a pixel or Y = MX for an image
• It’s Possible from this to Guess
– M, the basis comonent spectra in columns
– X , the strength of each component in each pixel
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–11
Non–Negative Least Squares
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–12
Northeastern University
Why Hyperspectral
South Bay of California; 101 curves down on the left.
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–2
Why Hyperspectral
Where are the Shrimp?
Hardware
– x, y on camera
∗ Slit, Grating and 2D Camera
· x, λ on camera
· y with time (pushbroom)
Applications
• Law Enforcement (Which crop is illegal?)
• Environmental (e.g. Deep Horizon)
rophores)
Forward Models
• Monte–Carlo
• Fluorescence
Some Common Absorbers
,Wavelength, nm
10 -4
10 -3
10 -2
10 -1
10 0
10 1
10 2
10 3
10 4
Some Common Fluorophores
, Wavelength, nm
488 514 633
Matrix Methods
• Forward Problem
Y = MX
• Typical Example
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–9
Variance, Covariance
• Covariance:
Y =
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–10
Variance, Covariance, Principal Components
• Arrange in Eigenvalue Order Decending
• Remember Y = MX for a pixel or Y = MX for an image
• It’s Possible from this to Guess
– M, the basis comonent spectra in columns
– X , the strength of each component in each pixel
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–11
Non–Negative Least Squares
June 2019 Chuck DiMarzio, Northeastern University 12286..slides7–12