Biomedical Applications of Scanning Probe Microscopy

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Biomedical Applications of Scanning Probe Microscopy. Dr James R Smith School of Pharmacy and Biomedical Science University of Portsmouth. Content. What is Scanning Probe Microscopy (SPM) ? Principles of SPM How does it differ from SEM/TEM ? SPM Facilities at Portsmouth - PowerPoint PPT Presentation

Transcript of Biomedical Applications of Scanning Probe Microscopy

  • Biomedical Applications of Scanning Probe MicroscopyDr James R SmithSchool of Pharmacy and Biomedical ScienceUniversity of Portsmouth

  • ContentWhat is Scanning Probe Microscopy (SPM) ?Principles of SPMHow does it differ from SEM/TEM ?SPM Facilities at PortsmouthSelected Case StudiesSummaryQuestions

  • What is SPM ?SPM is a revolutionary technique which allows: 3-D imaging nanometre or micrometre scaleimaging in a variety of environmentswith minimal sample preparation

  • SPM TechniquesSPM is a generic name for a number of related probe techniques:Scanning Tunnelling Microscopy (STM)Atomic Force Microscopy (AFM)others, such as Scanning Thermal Microscopy (SThM), Scanning Electrochemical Microscopy (SECM), Magnetic Force Microscopy (MFM)

  • Historical BackgroundScanning Tunnelling Microscopy (STM) was first reported in 1982 by Binnig et al.Atomic Force Microscope (AFM) first appeared in 1986Commercial SPM instruments capable of STM and AFM operation available in 1992

  • Principles of AFM

  • Advantages of SPM over TEM and SEMNanometre/atomic resolutionAccurate height measurements to within 1 angstromThree-dimensional representation of imagesDoes not require UHVAbility to perform studies in aqueous environmentsManipulation of surfaces on sub-nanometre scale

  • Other Information from SPMSurface roughnessSurface areaHardness/softnessElasticityAdhesionFriction

  • Nanoindentation of Bacterial Cells

  • SPM Facilities at PortsmouthTopoMetrix Discoverer TMX2000 Modular SPM

  • Biomedical Applications of SPM at PortsmouthSurface metrication of hip prosthesesContact lens manufacture and foulingHair structure and diseaseBiocide actionPolymer binding to human cellsSurface roughness of skinRNA/DNA secondary structureanti-cancer drug design

  • Biodeterioration and ControlBiofilm contamination on an intraocular lensAction of a biocide on E. coli

  • Surface Metrication of Hip Prostheses

  • Soft Contact Lens ManufacturePigment distribution on a tinted soft contact lens (above) and surface roughness (left)Polypropylene injection mould

  • Human HairA=A-layer, B=exocuticle,C=endocuticle (above)Surface roughness/line profileSame hair sample imaged under watershowing swelling (right)

  • SummaryNanometre/atomic resolutionAccurate height measurements to within 1 angstromThree-dimensional representation of imagesDoes not require UHVAbility to perform studies in aqueous environmentsMinimal sample preparationMinimal damage to sampleManipulation of surfaces on sub-nanometre scale

  • AcknowledgementsDr S A Campbell - PortsmouthProf F C Walsh - PortsmouthDr B F Shahgaldi - St Thomas Hospital, LondonDr J A Swift - Unilever/De Montfort UniversityDr A Gough - Alberto-Culver Company (UK) LtdDr D H Morton - Clinic For Special Children, PhiladelphiaStaff and students at Portsmouth