Biology Presentation

Biology presentationBiology presentation

Chan Siu HongChan Siu Hong (24(24))

Lam Kwan ChekLam Kwan Chek (27(27))

Lee Nok HimLee Nok Him (30(30))

Lo HallLo Hall (34(34))

Wong Tsz KiWong Tsz Ki (42(42))

Group Group 8:8:

IntroductionIntroduction

Our group have bought a can of condeOur group have bought a can of condensed milk (nsed milk ( 煉奶煉奶 ) to test for the presenc) to test for the presence of glucose, reducing sugar, starch, fate of glucose, reducing sugar, starch, fats, proteins and vitamin C by different fos, proteins and vitamin C by different food tests.od tests.

PreparationPreparation We opened the can of condensed We opened the can of condensed milk and pour the contents into a milk and pour the contents into a beaker until the beaker is 50mL full. beaker until the beaker is 50mL full. Then add some water to dilute the Then add some water to dilute the milk. The condensed milk used in the milk. The condensed milk used in the tests are taken from here by using tests are taken from here by using dropper.dropper.

PreparationPreparation

Materials preparedMaterials prepared

1 bottle of distilled water1 bottle of distilled water

2 Clinistix papers2 Clinistix papers2 Albustix papers2 Albustix papers2 droppers2 droppers2 filter papers2 filter papers

1 tube of cooking oil1 tube of cooking oil1 bottle of iodine solution1 bottle of iodine solution1 boiling water bath1 boiling water bath1 bottle of DCPIP solution1 bottle of DCPIP solution1 spot plate1 spot plate1 bottle of Benedict’s solution1 bottle of Benedict’s solution1 beaker1 beaker

1 can of condensed milk1 can of condensed milk1 test tube rank1 test tube rank

10 test tubes10 test tubes

Tests orderTests orderTest for glucoseTest for glucose

Test for reducing sugarsTest for reducing sugars

Test for fatsTest for fats

Test for starchTest for starch

Test for vitamin CTest for vitamin C

Test for proteinsTest for proteins

1. Transfer a drop of condensed milk 1. Transfer a drop of condensed milk to the well of a spot plate. Also to the well of a spot plate. Also add another drop of distilled add another drop of distilled

water water to another well of the spot to another well of the spot plate, plate, which is used as a control. which is used as a control.

Steps:Steps:

First Test: Test for glucoseFirst Test: Test for glucose

2. Dip the test end of a Clinistix 2. Dip the test end of a Clinistix paper into the sample and paper into the sample and observe any colour change.observe any colour change.

Steps:Steps:


The colour changed from The colour changed from yellowyellow to to light light greengreen..


Result:Result:

After test for water (Original colour)

After test for condensed milk

Second Test: Test for reducing Second Test: Test for reducing sugarssugars

1. Mix equal volumes of condensed 1. Mix equal volumes of condensed milk and Benedict’s solution in a milk and Benedict’s solution in a test tube. Also prepare another test tube. Also prepare another test tube contains mixture oftest tube contains mixture of

distilled water distilled water and Benedict’sand Benedict’s

solution.solution.

Steps:Steps:

mixture of distilled water and Benedict’s solutionmixture of distilled water and Benedict’s solution

mixture of condensed milk and Benedict’s solution


2. Put the test tubes into a boiling 2. Put the test tubes into a boiling water bath. Boil the mixture for 5 water bath. Boil the mixture for 5 minutes and observe any change minutes and observe any change in in the mixture.the mixture.

Steps:Steps:


3. Stand the test 3. Stand the test tubes in a tubes in a

test test tube rank for tube rank for 15 minutes.15 minutes.

Steps:Steps:


The colour changed from The colour changed from light light blueblue to to orangeorange..

Result:Result:

Mixture of distilled water and Benedict’s solution (Original colour)

Mixture of condensed milk and Benedict’s solution

Third Test: Test for fatsThird Test: Test for fats

1. Transfer a drop of condensed milk 1. Transfer a drop of condensed milk to a filter paper and another drop to a filter paper and another drop of distilled water. Let the two of distilled water. Let the two

papers dry in papers dry in the air for 5 the air for 5 minutes.minutes.

Steps:Steps:


2. Put the two papers under the light. 2. Put the two papers under the light. Observe any translucent spot.Observe any translucent spot.

Steps:Steps:

The condensed milk spot becomes tranThe condensed milk spot becomes translucent.slucent.

Result:Result:


Cooking oil spot (translucent)

Condensed milk spot (translucent)

Fourth Test: Test for starchFourth Test: Test for starch

1. Transfer a drop of condensed milk 1. Transfer a drop of condensed milk to the well of a spot plate. Also to the well of a spot plate. Also transfer a drop of distilled transfer a drop of distilled water water

to to another well of the spot plate.another well of the spot plate.

Steps:Steps:


2. Add a drop of iodine solution to 2. Add a drop of iodine solution to the sample and observe any colour the sample and observe any colour

change.change.

Steps:Steps:

There is no visible change.There is no visible change.Result:Result:


Condensed milk with iodine solution

Distilled water with iodine solution

Fifth Test: Test for vitamin CFifth Test: Test for vitamin C

1. Add a few drops of DCPIP solution 1. Add a few drops of DCPIP solution in two test tubes.in two test tubes.

Steps:Steps:


2. Add in same amount of condensed 2. Add in same amount of condensed milk. Stir them well then. Also milk. Stir them well then. Also

add add another tube of distilled water.another tube of distilled water.

Steps:Steps:


3. Put the test tubes3. Put the test tubes in a boiling in a boiling water bath for 5 minutes. Then water bath for 5 minutes. Then put them into a beaker of cool put them into a beaker of cool water.water.

Steps:Steps:

The solution change from blue into The solution change from blue into light blue.light blue.

Result:Result:


Condensed milk solution

1. Transfer a drop of condensed milk 1. Transfer a drop of condensed milk to the well of a spot plate. Also to the well of a spot plate. Also add distilled water to another add distilled water to another

well well of the spot plateof the spot plate

Steps:Steps:

Sixth Test: Test for proteinsSixth Test: Test for proteins

2. Dip the test of an Albustix paper 2. Dip the test of an Albustix paper into the sample and observe any into the sample and observe any colour change.colour change.

Steps:Steps:


The Albustix paper turns from The Albustix paper turns from yellowyellow to to light greenlight green..

Result:Result:


After test for condensed milk

After test for distilled water (Original colour)

ConclusionConclusion

TestTest Original colourOriginal colour Final colourFinal colour MeaningMeaning

Clinistix paperClinistix paper YellowYellow Light greenLight green [[]Glucose]Glucose

Benedict’s testBenedict’s test Light blueLight blue OrangeOrange [[]Reducing sugar]Reducing sugar

Grease spot testGrease spot test TranslucentTranslucent [[]Oil]Oil

Iodine testIodine test BrownBrown BrownBrown [[]Starch]Starch

DCPIP testDCPIP test BlueBlue Light blueLight blue [[]Vitamin C]Vitamin C

Albustix paperAlbustix paper YellowYellow Light greenLight green [[]proteins]proteins

Thanks for watching!! Goodbye…

Biology Presentation

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