Biology Int 2 Section 2 revision.ppt

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    Cells & Enzymes

    Enzymes

    Made of proteinPresent in

    all living cells

    Converts substrates

    into products

    Biological

    catalysts

    Increase the rate of

    chemical reactionsRemain unchanged

    by chemical reaction

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    ProductSubstrate

    Chemical Reactions

    chemical reaction ta!es place "hen one or

    substances is chemically changed into one or more

    different substances#

    Chemical reactions ta!e place in cells all the time#

    Enzyme

    Catalase

    $ydrogen

    pero%ideater ' o%ygen

    Manganese

    dio%ide

    Catalyst

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    Catalysts

    catalyst is a substance "hich speeds up a chemicalreaction( "ithout itself being changed by the

    reaction#

    )# hen a catalyst is present( less energy is neededto get the chemical reaction started#

    *# hen a catalyst is present( the speed of the

    chemical reaction is faster#

    +# lthough a catalyst helps a chemical reaction to

    happen( it is unchanged at the end of the reaction#

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    Biological Catalysts

    ,he biological catalyst present in the cytoplasm ofplant and animal cells that speeds up the brea!do"n

    of hydrogen pero%ide is called C,-SE#

    $ydrogen

    pero%ide.%ygen ' "ater

    catalase

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    *#* /ature of Enzymes

    ,he biological catalyst present in the cytoplasm ofplant and animal cells that speeds up the brea!do"n

    of hydrogen pero%ide is called C,-SE#

    Catalase cannot speed up any other reaction# It is

    SPECI0IC to this reaction#

    Catalase$ydrogen

    pero%ideater ' o%ygen

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    .ne Enzyme 1 .ne Reaction

    ,here are thousands of different enzymes in yourbody#

    hy are there so many different enzymes2

    Each enzyme has its o"n uni3ue protein structure

    and shape( "hich is designed to match or

    C.MP-EME/, its substrate#

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    -oc! & 4ey $ypothesis

    n enzyme only acts on one type of substance( or substrate#

    ,herefore( the enzyme is said to be SPECI0IC to its one substrate#

    ,he shape of the active site 5binding site6 of the enzyme( matches the

    shape of the substrate# llo"ing the t"o molecules to bind during the

    chemical reaction#

    ,his theory of enzyme action is called the 7loc!8and8!ey9 hypothesis#

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    M.:IE

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    *#+ =sing a Control

    hy is it necessary to include a control e%perimentin an investigation2

    controlis an e%periment that allo"s a

    comparison "ithin an investigation in order toensure that the conclusions dra"n from the results

    are valid#

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    *#+ =sing a Control

    .nly one variable should be altered in aninvestigation( this is said to be the altered variable#

    It is important to ensure that all other potential

    variables are !ept the same in the investigation(

    these are called the controlled variables#

    hat "as the altered variable in the last

    investigation you carried out2

    hat "ere the controlled variables in the last

    investigation you carried out2

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    Enzymes involved in brea!do"n reactions

    Catalase$ydrogen pero%ide ater ' .%ygen

    mylase

    Starch Maltose

    -ipase0at 0atty acids ' >lycerol

    PepsinProtein mino acids

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    *#? Synthesis reactions

    >lucose is used by

    leaves as energy

    source or transportedto root for storage#

    -eaves ma!e glucose#

    Roots convert glucose

    into >8)8P#

    Phosphorylase

    converts >8)8P into

    starch#

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    *#? Synthesis reactions

    phosphorylase

    glucose-1-phosphate starch

    hat is phosphorylase2

    Phosphorylase is an enzyme that synthesises starch#

    hat is substrate of phosphorylase2

    >lucose8)8phosphatehat is the product2

    Starch

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    *#? Synthesis reactions

    phosphorylase

    glucose-1-phosphate starch

    mylase catalyses the brea!do"n of starch toproduce maltose#

    $o" is starch produced2

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    *#@ Effect of $igh ,emperature

    /otes on ;enaturation /otes on .ptimum temp

    A Optimum conditionsare the conditions

    at "hich an enzymes "or!s best

    A Rate of reactions may be affected by

    temperature and p$

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    *#@ Effect of $igh ,emperature

    /otes on ;enaturation/otes on

    .ptimum temp

    hat happens to the activity of an enzyme at high

    temperatures2

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    Movie

    Effect of p$ on enzymes

    hen the p$ changes out"ith optimal conditions( the shape

    of the active site of the enzyme alters and the enzyme is

    denatured#

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    Effect of p$ on enzyme activity

    Most enzymes "or! best at a p$ close to neutral 5p$6( but

    there are some e%ceptions# Pepsin( an enzyme found in thestomach( has an optimum p$ of *#

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    Enzyme Substrate Product5s6 ;egradationor synthesis2

    mylase Starch Maltose ;egradation

    Catalase $ydrogenpero%ide

    .%ygen and"ater

    ;egradation

    Pepsin Protein mino acids ;egradation

    Phosphorylase >lucose8)8

    phosphate

    Starch Synthesis

    -ipase 0at mino acids ;egradation

    Enzyme Summary