Biol 1020: A tour of the cell 6: A Tour of the Cell ... Compartments in eukaryotic cells ... cell...

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Chapter 6: A Tour of the Cell

Cell theory

Cell organization and homeostasis

Studying cells – microscopy and fractionation

Eukaryotic vs. prokaryotic cells

Compartments in eukaryotic cells (cell regions, organelles)

Cytoskeleton

Outside the cell

http://www.auburn.edu/academic/classes/biol/1020/bowling/

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• What are the main tenets of cell theory?

• What are the major lines of evidence that

all presently living cells have a common

origin?


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Cell theory

All living organisms are composed of cells

smallest “building blocks” of all multicellular organisms

all cells are enclosed by a surface membrane that separates them from other cells and from their environment

specialized structures with the cell are called organelles; many are membrane-bound


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Cell theory

Today, all new cells arise from existing cells

All presently living cells have a common origin

all cells have basic structural and molecular similarities

all cells share similar energy conversion reactions

all cells maintain and transfer genetic information in DNA

the genetic code is essentially universal


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• What are the main tenets of cell theory?

• What are the major lines of evidence that

all presently living cells have a common

origin?


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Cell theory





Cytoskeleton

Outside the cell


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• What is surface area to volume ratio,

and why is it an important consideration

for cells?

• What (usually) happens to surface area

to volume ratio as cells grow larger?


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Plasma membrane surrounds cells and separates their contents from the external environment

Cells are heterogeneous mixtures, with specialized regions and structures (such as organelles)


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Cell size is limited

surface area to volume ratio puts a limit on cell size

food and/or other materials must get into the cell

waste products must be removed from the cell

cells need a high surface area to volume ratio

BUT volume increases faster than surface area as cells grow larger…so cells usually must divide


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Fig. 5.4


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cell shape varies depending both on function and surface area requirements


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• What is surface area to volume ratio,

and why is it an important consideration

for cells?

• What (usually) happens to surface area

to volume ratio as cells grow larger?


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Cell theory





Cytoskeleton

Outside the cell


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• Compare and contrast:

– LM and EM

– SEM and TEM

• Include the terms resolution and

magnification in your discussions.


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Studying cells – microscopy

Most cells are large enough to be resolved from each other with light microscopes (LM)

cells were discovered by Robert Hooke in 1665

he saw the remains of cell walls in cork with a LM

his microscope had about 30x magnification

modern LMs can reach up to 1000x


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Fig. 5.2a


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Fig. 5.2b


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Fig. 5.2c


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LM resolution is limited

LM resolution (clarity) is limited

about 1 mm

due to the wavelength of visible light

only about 500 times better than the human eye, even at maximum magnification

small cells (such as most bacteria) are ~1 mm across, just on the edge of resolution

modifications of LMs and some treatments of cells allow observation of subcellular structure in some cases


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Resolution of most subcellular structure requires electron microscopy (EM)

electrons have a much smaller wavelength than light (resolve down to under 1 nm)

magnification up to 250,000x or more

resolution over 500,000 times better than the human eye


Lightmicroscope

Transmissionelectron

microscope

Scanningelectron

microscope

Light beam

Ocular lens

Objective lens

Specimen

Condenser lens

Light source

Electron gun

Electron beam

First condenserlens (magnet)

Specimen

Projectorlens (magnet)

Secondaryelectrons

Film or screen

Specimen

Second condenserlens

Scanning coil

Final (objective)lens

Cathode ray tubesynchronized with

scanning coil

Electrondetector

(a) (b) (c)

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transmission electron microscopy(TEM)

electron passes through sample

need very thin samples (100 nm or less thick)

samples embedded in plastic and sliced with a diamond knife


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scanning electron microscopy (SEM)

samples are gold-plated

electrons interact with the surface

images have a 3-D appearance


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• Compare and contrast:

– LM and EM

– SEM and TEM

• Include the terms resolution and

magnification in your discussions.


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• Which is SEM, and which TEM? How

can you tell?


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• Describe cell fractionation. Why is it

done, and how is it done? Include the

terms lyse, centrifugation, pellet, and

supernatant in your discussion.


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Studying cells – fractionation

Cells can be broken and fractionated to separate cellular components

cells are broken (lysed) by disrupting the cell membrane, often using some sort of detergent

grinding and other physical force may be required, especially if cell walls are present

centrifugation is used to separate cellular components


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centrifugation is used to separate cellular components

samples are spun at high speeds

results in a centrifugal force thousands to hundreds of thousands times “normal” gravity

after spinning:

pellet – what gets packed down to the bottom (densest material)

supernatant – solution above the pellet


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cell components will end up in either the pellet or the supernatant depending on their individual properties and the details of the centrifugation

intact membrane-bound organelles often wind up in pellets (denser once first)

special treatments can determine whether a component ends up in the pellet or supernatant


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density gradients can be used to subdivide pellet components

based on their density

can be used to better separate similar organelles from each other, for example Golgi complex from ER


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• Describe cell fractionation. Why is it

done, and how is it done? Include the

terms lyse, centrifugation, pellet, and

supernatant in your discussion.


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Cell theory





Cytoskeleton

Outside the cell


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• How do prokaryotic cells and eukaryotic

cells differ from each other in typical

size and general organization?

• Describe cytoplasm, cytosol,

nucleoplasm, and the general role of

membranes in cells.


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prokaryotic cellsdo not have internal membranes (thus no nuclear membrane)

main DNA molecule (chromosome) is typically circular; its location is called the nuclear area

other small DNA molecules (plasmids) are often present, found throughout the cell


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Eukaryotic vs. prokaryotic cells prokaryotic cells

plasma membrane is typically enclosed in a cell wall

often the cell wall is covered with a sticky layer of proteins and/or sugars called a capsule

do not completely lack organelles; have:

plasma membrane

ribosomes

generally just called bacteria

prokaryotic cells are typically 1-10 mm in diameter


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eukaryotic cellshave internal membranes and a distinct, membrane-enclosed nucleus

typically 10-100 mm in diameter


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• How do prokaryotic cells and eukaryotic

cells differ from each other in typical

size and general organization?

• Describe cytoplasm, cytosol,

nucleoplasm, and the general role of

membranes in cells.


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• List as many organelles as you can think

of. Describe their structures and key

functions.

• Draw and label a typical animal cell and

a typical plant cell, including organelles.


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• List as many organelles as you can think

of. Describe their structures and key

functions.

• Draw and label a typical animal cell and

a typical plant cell, including organelles.


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Cell theory





Cytoskeleton

Outside the cell


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How do proteins get outside of a cell?

How do proteins get into a cell membrane?

How does a cell digest its food?

How does a cell commit suicide?

Why would a cell commit suicide?


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• Describe the nuclear envelope, nuclear

pores, chromatin, chromosomes, and

nucleoli in terms of structures and key

functions.

• Name something that you KNOW must

get out of the nucleus for cells to

function.


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Compartments in eukaryotic cells

two general regions inside the cell: cytoplasm and nucleoplasm

cytoplasm – everything outside the nucleus and within the plasma membrane

contains fluid cytosol and organelles

nucleoplasm – everything within the nuclear membrane


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Compartments in eukaryotic cells

membranes separate cell regions

have nonpolar regions that help form a barrier between aqueous region

allow for some selection in what can cross a membrane (more details later)


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nucleus – the control center of the cell

typically large (~5 mm) and singular

nuclear envelope

double membrane surrounding the nucleus

nuclear pores –protein complexes that cross both membranes and regulate passage


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chromatin – DNA-protein complex

have granular appearance; easily stained for microscopy (“chrom-” = color)

“unpacked” DNA kept ready for message transcription and DNA replication

proteins protect DNA and help maintain structure and function

chromosomes – condensed or “packed” DNA ready for cell division (“-some” = body)


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nucleoli – regions of ribosome subunit assembly

appears different due to high RNA and protein concentration (no membrane)

ribosomal RNA (rRNA) transcribed from DNA there

proteins (imported from cytoplasm) join with rRNA at a nucleolus to form ribosome subunits

ribosome subunits are exported to the cytoplasm through nuclear pores

(note singular: nucleolus; plural: nucleoli)


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• Describe the nuclear envelope, nuclear

pores, chromatin, chromosomes, and

nucleoli in terms of structures and key

functions.

• Name something that you KNOW must

get out of the nucleus for cells to

function.


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• Describe the structure and function of

ribosomes.


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ribosomes – the sites of protein synthesis

ribosomes are granular bodies with three RNA strands and about 75 associated proteins

two main subunits, large and small

perform the enzymatic activity for forming peptide bonds, and serve as the sites of translation of genetic information into protein sequences


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ribosomes – the sites of protein synthesis

prokaryotic ribosome subunits are both smaller than the corresponding subunits in eukaryotes

in eukaryotes

the two main subunits are formed separately in the nucleolus and transported separately to the cytoplasm

some are free in the cytoplasm while others are associated with the endoplasmic reticulum (ER)


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• Describe the structure and function of

ribosomes.


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• What is the endomembrane system

(include organelle components)?


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• Diagram the cisternal maturation model for the Golgi.

• Diagram and describe the pathway from synthesis to final destination for a secreted protein. Then do the same for a plasma membrane protein.

• Describe the structure and function of: - ER - microbodies in general

- vesicles - lysosomes

- vacuoles - peroxisomes

- Golgi apparatus - glyoxysomes


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endomembrane system

endomembrane system – a set of membranous organelles that interact with each other via vesicles

includes ER, Golgi apparatus, vacuoles, lysosomes, microbodies, and in some definitions the nuclear membrane and the plasma membrane


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endomembrane system

endoplasmic reticulum (ER) – membrane network that winds through the cytoplasm

winding nature of the ER provides a lot of surface area

many important cell reactions or sorting functions require ER membrane surface

ER lumen – internal aqueous compartment in ER

separated from the rest of the cytosol

typically continuous throughout ER and with the lumen between the nuclear membranes

enzymes within lumen and imbedded in lumen side of ER differ from those on the other side, thus dividing the functional regions


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endomembrane system

smooth ER – primary site of lipid synthesis, many detoxification reactions, and sometimes other activities

rough ER – ribosomes that attach there insert proteins into the ER lumen as they are synthesized


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endomembrane system

rough ER – ribosomes that attach there insert proteins into the ER lumen as they are synthesized

ribosome attachment directed by a signal peptide at the amino end of the polypeptide (see Ch. 17.4, p.326)

a protein/RNA signal recognition particle (SRP) binds to the signal peptide and pauses translation

at the ER the assembly binds to an SRP receptor protein

SRP leaves, protein synthesis resumes (now into the ER lumen), and the signal peptide is cut off


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endomembrane system

proteins inserted into the ER lumen may be membrane bound or free

proteins are often modified in the lumen (example, carbohydrates or lipids added)

proteins are transported from the ER in transport vesicles

vesicles – small, membrane-bound sacs

buds off of an organelle (ER or other)

contents within the vesicles (often proteins) transported to another membrane surface

vesicles fuses with membranes, delivering contents to that organelle or outside of the cell


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Fig. 5.16d (TEArt)

Vesiclebuddingfrom roughendoplasmicreticulum

Fusionof vesiclewith Golgiapparatus

Migratingtransportvesicle

Protein

Ribosome


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endomembrane system

Golgi apparatus (AKA Golgi complex) – a stack of flattened membrane sacs (cisternae) where proteins further processed, modified, and sorted [the “post office” of the cell]

not contiguous with ER, and lumen of each sac is usually separate from the rest

has three areas: cis, medial, and trans


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endomembrane system

cis face: near ER and receives vesicles from it; current model (cisternal maturation model) holds that vesicles actually coalesce to continually form new cis cisternae

medial region: as a new cis cisterna is produced, the older cisternae mature and move away from the ER

in this region proteins are further modified (making glycoproteins and/or lipoproteins where appropriate, and )

maturing cisternae may make other products; for example, many polysaccharides are made in the Golgi

some materials are needed back a the new cis face and are transported there in vesicles

trans face: nearest to the plasma membrane; a fully matured cisterna breaks into many vesicles that are set up to go to the proper destination (such as the plasma membrane or another organelle) taking their contents with them


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endomembrane system


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endomembrane system

lysosomes – small membrane-bound sacs of digestive enzymes

serves to confine the digestive enzymes and their actions

allows maintenance of a better pH for digestion (often about pH 5)

formed by budding from the Golgi apparatus; special sugar attachments to hydrolytic enzymes made in the ER target them to the lysosome


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endomembrane system lysosomes – small membrane-bound sacs of digestive enzymes

used to degrade ingested material, or in some cases dead or damaged organelles

ingested material is found in vesicles that bud in from the plasma membrane; the complex molecules in those vesicles is then digested

can also fuse with dead or damaged organelles and digest them

digested material can then be sent to other parts of the cell for use

found in animals, protozoa; debatable in other eukaryotes, but all must have something like a lysosome


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endomembrane system vacuoles – large membrane-bound sacs

that perform diverse roles; have no internal structure

distinguished from vesicles by size

in plants, algae, and fungi, performs many of the roles that lysosomes perform for animals

central vacuole – typically a single, large sac in plant cells that can be 90% of the cell volume

usually formed from fusion of many small vacuoles in immature plant cells

storage sites for water, food, salts, pigments, and metabolic wastes

important in maintaining turgor pressure

tonoplast – membrane of the plant vacuole

food vacuoles – present in most protozoa and some animal cells; usually bud from plasma membrane and fuse with lysosomes for digestion

contractile vacuoles – used by many protozoa for removing excess water


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endomembrane system

microbodies – small membrane-bound organelles that carry out specific cellular functions; examples:

lysosomes could be consider a type of microbody

peroxisomes – sites of many metabolic reactions that produce hydrogen peroxide (H2O2), which is toxic to the rest of the cell

peroxisomes have enzymes to break down H2O2, protecting the cell

peroxisomes are abundant in liver cells in animals and leaf cells in plants

normally found in all eukaryotes

example: detoxification of ethanol in liver cells occurs in peroxisomes

glyoxysomes – in plant seeds, contains enzymes that convert stored fats into sugar


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• What is the endomembrane system

(include organelle components)?


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• Diagram the cisternal maturation model for the Golgi.

• Diagram and describe the pathway from synthesis to final destination for a secreted protein. Then do the same for a plasma membrane protein.

• Describe the structure and function of: - ER - microbodies in general

- vesicles - lysosomes

- vacuoles - peroxisomes

- Golgi apparatus - glyoxysomes


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Energy Converting Organelles

energy obtained from the environment is typically chemical energy (in food) or light energy

mitochondria are the organelles where chemical energy is placed in a more useful molecule

chloroplasts are plastids where light energy is captured during photosynthesis


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• Draw a mitochondrion in cross-section

and describe its structure and functions.


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mitochondria – the site of aerobic respiration

recall aerobic respiration:

sugar + oxygen carbon dioxide + water + energy

the “energy” is actually stored in ATP


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Energy Converting Organelles -mitochondria

mitochondria have a double membrane

space between membranes = intermembrane space

inner membrane is highly folded, forming cristae; provides a large surface area

inner membrane is also a highly selective barrier

the enzymes that conduct aerobic respiration are found in the inner membrane

inside of inner membrane is the matrix, analogous to the cytoplasm of a cell


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Energy Converting Organelles –mitochondria

mitochondria have their own DNA, and are inherited from the mother only in humans

mitochondria have their own division process, similar to cell division; each cell typically has many mitochondria, which can only arise from mitochondrial division

some cells require more mitochondria than others

mitochondria can leak electrons into the cell, allowing toxic free radicals to form

mitochondria play a role in initiating apoptosis(programmed cell death)


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• Draw a mitochondrion in cross-section

and describe its structure and functions.


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• Draw a chloroplast in cross-section and

describe its structure and functions.


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plastids – organelles of plants and algae that produce and store food

include amyloplasts (for starch storage), chromoplasts (for color, often found in petals and fruits), and chloroplasts (for photosynthesis)

like mitochondria, have their own DNA (typically a bit larger and more disk-shaped than mitochondria, however)

derive from undifferentiated proplastids, although role of mature plastids can sometimes change

numbers and types of plastids vary depending on the organism and the role of the cell


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Energy Converting Organelles – plastids

(chloroplasts)

chloroplasts get their green color from chlorophyll, the main light harvesting pigments involved in photosynthesis

carbon dioxide + water + light energy food (glucose) + oxygen

chloroplasts have a double membrane

the region within the inner membrane is the stroma; it is analogous to the mitochondrial matrix

inner membrane is contiguous with an interconnected series of flat sacks called thylakoids that are grouped in stacks called grana

the thylakoids enclose aqueous regions called the thylakoid lumen

chlorophyll is found in the thylakoid membrane, and the reactions of photosynthesis take place there and in the stroma

carotenoids in the chloroplast serve as accessory pigments for photosynthesis


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• Draw a chloroplast in cross-section and

describe its structure and functions.


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• Describe the endosymbiont theory.

Include evidence for it, including

predictions that have proven true.


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Energy Converting Organellesendosymbiont theory

mitochondria and plastids evolved from prokaryotic cells that took residence in larger cells and eventually lost their independence

the cells containing the endosymbionts became dependent upon them for food processing, and in turn provide them with a protected and rich environment (a mutualistic relationship)


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supporting evidence

the size scale is right - mitochondria and plastids are on the high end of the size of typical bacteria

endosymbionts also have their own DNA and their own “cell” division; in many ways they act like bacterial cells

the DNA sequence and arrangement (circular chromosomes)of endosymbionts is closer to that of bacteria than to that found in the eukaryotic nucleus

endosymbionts have their own ribosomes, which are much like bacterial ribosomes

there are other known, more modern endosymbiotic relationships: algae in corals, bacteria within protozoans in termite guts


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some genes appear to have been shuttled out of the endosymbionts to the nucleus

many of the proteins used by endosymbionts are actually encoded by nuclear genes and translated in the cytoplasm (or on rough ER) and transported to the endosymbionts

DNA sequencing of endosymbionts is being used to trace the evolutionary history of the endosymbionts

appears that endosymbiosis began about 1.5 to 2 billion years ago (around when the first eukaryotic cells appeared)

mitochondria appear to have a monophyletic origin (one initial endosymbiotic event, giving rise to all mitochondria in eukaryotic cells today)

plastids appear to have a polyphyletic origin (more than one initial endosymbiotic event giving rise to different plastid lines present today in algae and plants)


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• Describe the endosymbiont theory.

Include evidence for it, including

predictions that have proven true.


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Cell theory





Cytoskeleton

Outside the cell


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• What are the functions of the

cytoskeleton?

• What are the three main types of

cytoskeleton? Describe the structure

and function(s) of each type.


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• Describe the structure and function(s)

of:

– motor proteins

– MTOCs

– centrosomes

– centrioles

– cilia and flagella


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Cytoskeleton eukaryotic cells typically have a size and shape that is maintained

the cytoskeleton is a dense network of protein fibers that provides needed structural support

the network also has other functions

a scaffolding for organelles

cell movement and cell division (dynamic nature to the protein fibers is involved here)

transport of materials within the cell

the cytoskeleton is composed of three types of protein filaments: microtubules, microfilaments, and intermediate filaments


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microtubules are the thickest

filaments of the cytoskeleton hollow, rod -shaped cylinders

about 25 nm in diameter

made of a-tubulin and b-tubulindimers

dimers can be added or removed from either end (dynamic nature)

one end (plus end) adds dimers more rapidly than the minus end

can be anchored, where an end is attached to something and can no longer add or lose dimers


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microtubule-organizing centers

(MTOCs) serve as anchors centrosome in animal

cells

centrosome has two centrioles in a perpendicular arrangement

centrioles have a 9x3 structure: 9 sets of 3 attached microtubules forming a hollow cylinder

used for assembly of microtubules for use throughout the cell


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microtubule-organizing centers

(MTOCs) serve as anchors

centrioles are duplicated before cell division

play an organizing role for microtubule spindles in cell division

other eukaryotes must use some alternative MTOC during cell division; still incompletely described


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microtubules are involved in

moving organelles motor proteins (such as

kinesin or dynein) attach to organelle and to microtubule

using ATP as an energy source, the motor proteins change shape and thus produce movement

microtubule essentially acts as a track for the motor protein

motor proteins are directional; kinesin moves toward the plus end, dynein away from it


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cilia and flagella are made of

microtubules thin, flexible projections from cells

used in cell movement, or to move things along the cell surface

share the same basic structure; called cilia if short (2-10 mm typically) and flagella if long (typically 200 mm)

central stalk covered by cell membrane extension, and anchored to a basal body

9x3 structure

stalk has two inner microtubules surrounded by nine attached pairs of microtubules

9+2 arrangement


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cilia and flagella are made of

microtubules

stalk has two inner microtubules surrounded by nine attached pairs of microtubules

9+2 arrangement

dynein attached to the outer pairs actually fastens the pair to its neighboring pair

dynein motor function causes relative sliding of filaments; this produces bending movement of the cilium or flagellum


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microfilaments are solid filaments


composed of two entwined chains of actin monomers

linker proteins cross-link the actin chains with each other and other actin associated proteins

actin monomers can be added to lengthen the microfilament or removed to shorten it; this can be used to generate movement


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microfilaments are solid filaments


important in muscle cells; in conjunction with myosin, they are responsible for muscle contraction

used for many cell movements such as:

contractile structures

forming cell extensions

“pinching in” during cell division


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intermediate filaments

typically just a bit wider than microfilaments, this is the catch-all group for cytoskeletal filaments composed of a variety of other proteins

the types of proteins involved differ depending on cell types and on the organism; apparently limited to animal cells and protozoans


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intermediate filaments not easily disassembled, thus more permanent

a web of intermediate filaments reinforces cell shape and positions of organelles (they give structural stability)

prominent in cells that withstand mechanical stress

form the most insoluble part of the cell


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• What are the functions of the

cytoskeleton?

• What are the three main types of

cytoskeleton? Describe the structure

and function(s) of each type.


.

• Describe the structure and function(s)

of:

– motor proteins

– MTOCs

– centrosomes

– centrioles

– cilia and flagella


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Cell theory





Cytoskeleton

Outside the cell


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• Describe the outer part and outside

interface of a:

– typical prokaryotic cell

– typical plant cell

– typical fungal cell

– typical animal cell

• Diagram and describe the animal cell

glycocalyx and ECM interaction (include

collagen, fibronectin, and integrin).


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Outside the Cell Most prokaryotes have a cell wall, an outer envelope, and a

capsule (capsule is also called glycocalyx or cell coat)

Most eukaryotic cells produce materials that are deposited outside the plasma membrane but that remain associated with it


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Outside the Cell plants have thick, defined cell

walls made primarily of cross-linked cellulose fibers

growing plant cells secrete a primary cell wall, which is thin and flexible

growth ends primary cell wall is usually thickened and solidified

often a secondary cell wall is then produced between the primary cell wall and the plasma membrane

still contains cellulose

typically has more strengthening material (for example, lignin in wood)


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Outside the Cell

fungi typically have thinner cell walls than plants, made primarily of cross-linked chitinfibers


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Outside the Cell animals do not have cell walls, but their cells secrete

varying amounts of compounds that can produce a glycocalyx and an extracellular matrix (ECM)

glycocalyx: polysaccharides attached to proteins and lipids on the outer surface of the plasma membrane

typically functions in cell recognition and communication, cell contacts, and structural reinforcement

often works through direct interaction with the ECM


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Outside the Cell ECM: a gel of carbohydrates and fibrous proteins;

several different molecules can be involved

main structural protein is tough, fibrous collagen

fibronectins are glycoproteins in the ECM that often bind to both collagen and integrins

integrins are proteins in the plasma membrane that typically receive signals from the ECM


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• Describe the outer part and outside

interface of a:

– typical prokaryotic cell

– typical plant cell

– typical fungal cell

– typical animal cell

• Diagram and describe the animal cell

glycocalyx and ECM interaction (include

collagen, fibronectin, and integrin).


Biol 1020: A tour of the cell 6: A Tour of the Cell ... Compartments in eukaryotic cells ... cell...

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Transcript of Biol 1020: A tour of the cell 6: A Tour of the Cell ... Compartments in eukaryotic cells ... cell...