Bioinformatics Analysis of Nucleotide Sequences
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Transcript of Bioinformatics Analysis of Nucleotide Sequences
BIOINFORMATICS ANALYSIS OF NUCLEOTIDE SEQUENCES
Members:
• Avellaneda Vergara Adrian Gustavo• Yarasca Cerna Withney Aracely • Zegarra Aguinaga Janeth Alexandra• Farfan Hernandez Kevin Jhonny• Graham Angeles Laura Andrea
INTRODUCTION
• DNA sequencing is a technique that provides a detailed analysis of the structure of DNA and
consists of a set of techniques and biochemical methods that allow us to determine the
sequence of nucleotides (A, C, G, and T) analysis is DNA.
• In the mid-1970s happened a revolution in technology for identifying DNA sequence. In
1977 was published the complete nucleotide sequence of a viral genome (φ X174, 5375
nucleotides long). This milestone in molecular biology occurred in the laboratory of
Frederick Sanger, who identified the amino acid sequence of the polypeptide (insulin) 25
years earlier.
• Bioinformatics is the application of computer technology to information in molecular
biology, encompassing aspects of the acquisition, processing, distribution, analysis,
interpretation and integration of biological information. There are several databases that
organize information and they are often used, which are presented in the following
bioinformatics centers: GenBank (NCBI) and BOLD Systems
• The NCBI database (established in 1988) has a public database, with three components.
Creating databases (store biological data), development of algorithms and statistics to
determine relationships between databases, and use these tools to analyze and interpret
various types of biological data (sequences of DNA, RNA, protein, protein structure, gene
expression, biochemical pathways)
• The Barcode of Life Data Systems (BOLD) is an informatics workbench aiding the
acquisition, storage, analysis, and publication of DNA barcode records. By assembling
molecular, morphological, and distributional data, it bridges a traditional bioinformatics
chasm. BOLD is freely available to any researcher with interests in DNA barcoding. By
providing specialized services, it aids the assembly of records that meet the standards
needed to gain BARCODE designation in the global sequence databases. Because of its web-
based delivery and flexible data security model, it is also well positioned to support projects
that involve broad research alliances.
PROCEDURE(SCREENSHOTS)
To do the analysis of nucleotides we must follow many steps.
STEP 1
STEP 2
Then we have to go to Edit > reverse + complement
STEP 3
STEP 4
• We still in the same sequence, but we are modifying other parts.
STEP 5
STEP 6
STEP 7
STEP 8
STEP 9
• After saved all our sequences in the notepad we have to go to BioEdit
program.
STEP 10
STEP 11
STEP 12
• Then we must create the consensus sequence, so we go to the
option aligment
STEP 13
• We get the consensus sequence and the differtens between some
nucleotides
STEP 14
STEP 15
• Finally we have all the step to do the research of our specie in the
different data bases that exist (NCBI & BOLDsystem)
STEP 16
STEP 17
STEP 18
• Then we have our result…
STEP 19
• Whit this information we find out which kind of living beings is.
STEP 20
• Here we have all the possibilities for our specie.
STEP 21
STEP 22
• After foud all the information about our specie we must go to
BOLDsystem to get detailed information
STEP 23
STEP 24
STEP 25
STEP 26
Then we select, in this case 20, differet sequences that are similar
with our consensu sequence. We can see the similarity in the ítem
IDENT that is in red.
STEP 27
STEP 28
• Then we go to NotePad
STEP 29
STEP 30
Then open the BioEdit program with the other nucleotides
sequences
STEP 31
STEP 32
STEP 33
STEP 34
STEP 35
STEP 36
STEP 37
STEP 38
STEP 39
STEP 40
STEP 41
STEP 42
STEP 43
STEP 44
STEP 45
STEP 46
STEP 47
STEP 48
STEP 49
STEP 50
• The Analysis of result and the conclusions are in the other file because is too long.