Bioengineering with (only) DNA
description
Transcript of Bioengineering with (only) DNA
![Page 1: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/1.jpg)
Bioengineering with (only) DNA
Dave Zhang
2nd Annual Unither Nanomedical and Telemedical Technology Conference
26 Feb 2009
![Page 2: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/2.jpg)
DNA vs. genetic engineering
Genetic engineering DNA engineering
A. Z. Ansari, Nature Chemical Biology,
2007Nanoengineer, by Nanorex
http://nanoengineer-1.com/content/index.php?option=com_content&task=view&id=37&Itemid=47
![Page 3: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/3.jpg)
DNA abstraction
Michael Ströck,
http://en.wikipedia.org/wiki/File:DNA_Overview.png
![Page 4: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/4.jpg)
Structures
CiliaCell wall Actin and myosin
C. Daghlian,http://refm.dartmouth.edu/images/mammalianLungSEM/source/9.html
Lacey Samuels,
https://www.biomedia.cellbiology.ubc.ca/cellbiol/user/scripts/qry_media_id.php?media_id=212
http://online-media.uni-marburg.de/histologie/introhis/HIS/txt/muscsem/musc01_sem.htm
![Page 5: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/5.jpg)
E. Winfree, F. Liu, L.A. Wenzler, N.C. Seeman, Nature 1998Y. He, T. Ye, M. Su, C. Zhang, A.E. Ribbe,
W. Jiang, C. Mao, Nature 2008P. Rothemund, Nature 2006
Two-dimensional latticeThree-dimensional shapes
Aperiodic structures
2 um x 8 um500,000 tiles!
Structures
![Page 6: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/6.jpg)
Logic and actuators
Amoeba
Micrographia,http://www.micrographia.com/specbiol/protis/homamoeb/amoe0100/amoeba21.htm
Algae
Yum! Granite
![Page 7: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/7.jpg)
Boolean Logic
Logical processing
![Page 8: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/8.jpg)
D. Y. Zhang, in preparation
Input 1 Input 2
Logical AND gate substrate
Output
Logical processing
![Page 9: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/9.jpg)
D. Y. Zhang, in preparation
Logical processing
![Page 10: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/10.jpg)
Logical processing
NAND gate is universal!
D. Y. Zhang, in preparation
![Page 11: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/11.jpg)
Actuators and motors
B. Yurke, A.J. Turberfield, A.P. Mills, F. C. Simmel, J.L.
Neumann, Nature 2000
![Page 12: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/12.jpg)
Amplification and reproduction
http://www.bunny-rabbits.com/http://www.kindplanet.org/thesanctuary.html
Jeff Hardin,http://worms.zoology.wisc.edu/dd2/echino/cleavage/intro.html
![Page 13: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/13.jpg)
Amplification and reproduction
![Page 14: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/14.jpg)
Spontaneous generationNot really alive:more like crystallization in a supersaturated solution
Cons:
Pro:If we know what we’re looking for, we can amplify its concentration dynamically.
Amplification and reproduction
D. Y. Zhang, A.J. Turberfield, B. Yurke, E. Winfree, Science
2007
![Page 15: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/15.jpg)
Amplification and reproduction
![Page 16: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/16.jpg)
Interaction with general chemistry
Adapted from David Liu’s website:http://evolve.harvard.edu/rschdts.htm#DTS
Z. J. Gartner, B. N. Tse, R. Grubina, J.B. Doyon, T.M. Snyder, D.R. Liu, Science 2004
Small organic group
Boom!Boom!
![Page 17: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/17.jpg)
DNA engineering as a nanomedical tool
•Instrument-free disease detection
•Embedded control of apoptosis
•Control of developmental biology
![Page 18: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/18.jpg)
Parting Thought
http://www.kurzweilai.net/articles/images/Carlson(PaceAndProliferation)figure1.gif
![Page 19: Bioengineering with (only) DNA](https://reader035.fdocuments.net/reader035/viewer/2022062322/568143d5550346895db061f1/html5/thumbnails/19.jpg)
Thanks for listening!
(and thanks to the following for their money:)