biocrops biotechnology limited: Yam multiplication project
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Transcript of biocrops biotechnology limited: Yam multiplication project
Biocrops Biotechnology Limited: Yam Multiplication project
Ms. Adaora EkwegbaraYIIFSWA Technical officer. Biocrops
Ltd
Pioneers of Commercial Biotechnology
in NigeriaBoard Members Senior staff
Biocrops at a Glance• Biocrops, established in 2000, is the premier biotechnology company in Nigeria.• Biocrops operates at two centers located in Lagos and Abuja.
Plantation crops being promoted by Biocrops include:-
SugarcaneCocoaOil PalmCottonPineapplesEucalyptusCassavaSweet potatoYamBananaPlantain
Biocrops in
pictures
Biocrops has established the industry standard for the size of sugarcane seedling for field deployment
Yam multiplication at Biocrops
Picture showing the largest Immersion Bioreactor in Nigeria
Strategies to produce Yam foundation seeds at the Abuja Biofactory
• Low-Hanging-Fruit technologies:-–Conventional Plant tissue culture–Temporary Immersion Bioreactor
Technology–Aeroponics Technology–Yam Miniset technology–Somatic embrogenesis –Vine rooting technology
Progress Made Under YIIFSWA
• Year 1 plants are still growing, making them 2 years old!!
• Screen house repaired with shade nets replaced.
• Biocrops has been backstopped with vines to replant most of the wells in the aeroponics facility
• Aeroponic systems has overcome initial heat-related problems and is now functional
Date cultured
Initial No. of single nodes/vessel
Variety After 4 weeks No of nodes/expla
nt
8 weeks after culturing
Replicate
08/12/2015 4 (100 Vessels)
TDa 2014 4 7 28
08/12/2015 4 (15 vessels)
TDr 95/19158
4 6 6
09/12/2015 4 (10 vessels)
TDa 291 3 5 5
09/12/2015 4 (20 vessels)
TDr 89/02665
4 7 28
Tissue Culture
Date of culture
Initial No. of single nodes/vessel
Variety After 4 weeks No of nodes/explant
8 weeks after culturing
Replicate
17/08/2015 4 (10 vessels)
TDa 2014
3 6 24
17/08/2015 4 (6 vessels)
TDr 95/19158
3 6 24
18/08/2015 4 (4 vessel)
TDr EKPE
2 4 16
17/08/2015 4 (8 vessels)
TDr 89/02665
4 6 24
17/08/2015 4 (6 vessels)
TDa 291 3 6 24
Bioreactor
Date Planted
Source Variety No Planted
No surviving after 1 week
No surviving after 2 week
No surviving after 4 week
Tuber weight at harvest
25/06/2015 Vine cutting TDa 98/01166 25 19 19 19 025/06/2015 Vine cutting TDr Amula 38 24 24 24 025/06/2015 Vine cutting TDr Alumaco 7 7 7 7 016/07/2015 Rooted vine TDa 00/00194 12 12 12 12 0
16/07/2015 Rooted vine TDa 98/01176 13 11 9 9 016/07/2015 Rooted vine TDa 95/19158 17 17 17 17 0
21/07/2015 Vine cutting TDr 95/18544 32 25 19 19 021/07/2018 Vine cutting TDa 98/01176 10 6 4 4 021/07/2015 Vine cutting TDr 95/19177 56 40 31 31 027/07/2015 Rooted vine TDr 95/19177 13 13 13 13 027/07/2015 Rooted vine TDa 98/01176 16 16 16 16 001/09/2015 Rooted
aeroponics vine TDr 95/18544 11 11 11 11 0
01/09/2015 Rooted aeroponics vine
TDr 95/19158 14 13 13 13 0
Aeroponics
Aeroponics Works!!!
Harvested tubers
TubersVariety/ weight of tuber(grams)
TDr 95/18544(54 tubers)
TDr 95/19158(1 tuber)
TDr 95/19177(8 tubers)
TDr Ekpe(8 tubers)
TDr Pepa(11 tubers)
TDa 2014(9 tubers)
44.59 9.9 4.00 0.5 4.1 0.414.19 4.68 0.3 4.4 0.179.76 1.28 1.3 6.8 0.4516.56 0.90 0.6 2.4 0.255.37 0.44 0.4 0.8 0.266.60 0.43 0.3 1.9 0.389.72 0.23 0.3 0.8 0.198.34 0.22 0.3 1.6 0.187.11 0.8 0.166.01 0.5 4.98 0.4
8.21
Biocrops Targets
•Biocrops targets to expand its Temporary Immersion Bioreactor to 1000 vessels by Dec, 2016•Biocrops targets to plant 1 hectare of quality declared seeds in 2016 planting season.•Biocrops targets to cover the entire 1960 wells in its aeroponics facility by July, 2016.•These targets should give 10,000 quality declared seeds by Dec, 2016
Key needs• Licensing by the National Agricultural Seeds
Council!!!• Training of staff on clean seed technology: viz
virus indexing/expansion of training on bacteria indexing, somatic embryogenesis etc.
• Assistance in materials sourcing. Quality product suppliers
• Generation of demand for yam seeds! Demand would stimulate commercial production!
Key Request!
A guaranteed market for yam seedlings:- a purchase agreement for
Yam seedlings!!!! Biocrops targets to produce up to
200,000 yam seeds/annum.
THANK YOU!.
THANK YOU!
THANK YOU!