Biochromatography HPLC Columnsgeya99.com/literature/chroma_cat/HPLC Chromatography/Biochrom… ·...

Biochromatography

HPLC Columns

With the partnership of Teknokroma

1 9000 Varna , Pop Hariton No.47 | e-mail : [email protected] | www.geya99.com

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BIOCHROMATOGRAPHY HPLC COLUMNS

ICSep AN1

TosoBioScience offer the largest range of high efficiency columns for the analysis and separation of biomolecules using the chromatographic technique of anionic and cationic interchange. These columns, with silica or methacrylate spherical packings, with controlled porosity and substituted with different interchange groups allow the resolution of proteins, peptides, oligonucleotides derived from DNA and RNA and other fragments of nucleic acids. We also include a line of polymeric packings of polystyrenedivinylbenzene appropriately substituted for the separation and analysis of low molecular weight molecules such as sugars, aminoacids,etc.

Reference Exchange Material Based Description

TH-19685

Anion PEEK Column BioAssist Q, 4000 A 10 ?, 4.6 mm x 5.0 cm

TH-19686

Cation PEEK BioAssist S, 1300 A 7 ?, 4.6 mm x 5.0 cm

TH-19308

Cation Guard Column Cartridge

TH-19308

Anion Guard Column Cartridge 2.0 mm x 1.5 cm

TH-07167

Cation Stainless steel Silic CM-2SW, 125 A 5 ?, 4.6 mm x 25.0 cm

TH-07162


TH-13069

Cation Guard Column CM-5PW Guardgel Kit

TH-14024

Cation Glass Guard Column CM-5PW Guardgel Kit

TH-16094

Cation Guard Column CM-5PW Prep Guardgel Kit

TH-14068

Cation Guard Column CM-5PW Prep Guardgel Kit

TH-14010

Cation Glass Polymer CM-5PW, 1000 A 10 ?, 5.0 mm x 5.0 cm

TH-13068

Cation Stainless steel Polymer CM-5PW, 1000 A 10 ?, 7.5 mm x 7.5 cm

TH-14011


TH-14012


TH-14021

Cation Stainless steel Polymer CM-5PW, 1000 A 13 ?, 21.5 mm x 15.0 cm

TH-07650

Cation Guard Column CM-SW Guardgel Kit

TH-19685

Anion PEEK Column BioAssist Q, 4000 A 10 ?, 4.6 mm x 5.0 cm

TH-19686

Cation PEEK BioAssist S, 1300 A 7 ?, 4.6 mm x 5.0 cm

TH-19308

Cation Guard Column Cartridge

TH-19308

Anion Guard Column Cartridge 2.0 mm x 1.5 cm

TH-07167


TH-42154

Anion Guard Column DEAE-2SW Cartridge 5 ?, 2.0 mm x 1.0 cm

TH-18761

Anion Stainless steel Silic DEAE-2SW, 125 A 5 ?, 2.0 mm x 25.0 cm

TH-07168


TH-07163


TH-07928

Anion Guard Column DEAE-5PW 20 ?, 45.0 mm x 5.0 cm

TH-18757

Anion Stainless steel Polymer DEAE-5PW , 1000 A 10 ?, 2.0 mm x 7.5 cm

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TH-13061

Anion Glass Polymer DEAE-5PW , 1000 A 10 ?, 5.0 mm x 5.0 cm

TH-07164


TH-08802


TH-14016


TH-07574


TH-07930


TH-14466

Anion Guard Column DEAE-5PW , Kit 13 ?, 20.0 mm x 2.0 cm

TH-42152

Anion Guard Column DEAE-5PW Cartridge, Kit 10 ?, 2.0 mm x 1.0 cm

TH-07210

Anion Guard Column DEAE-5PW Guardgel, Kit 20 ?

TH-08806

Anion Glass Guard Column DEAE-5PW Guardgel, Kit, 20 ?

TH-16092

Anion Guard Column DEAE-5PW Prep Guardgel, Kit, 20 ?

TH-17088

Anion Guard Column DEAE-NPR , 5 ?, 4.6 mm x 0.5 cm

TH-13075

Anion Stainless steel Polymer DEAE-NPR, nonporus, 2.5 ?, 4.6 mm x 3.5 cm

TH-07648

Anion Guard Column DEAE-SW Guardgel Kit, 20 ?

TH-18253

Anion Guard Column DNA-NPR , 5 ?, 4.6 mm x 0.5 cm

TH-18249

Anion Stainless steel Polymer DNA-NPR, nonporus, 2.5 ?, 4.6 mm x 7.5 cm

TH-42151

Anion Stainless steel Polymer DNA-NPR, nonporus, 2.5 ?, 7.5 mm x 7.5 cm

TH-16653

Cation Stainless steel Polymer Oapak-A, 1000 A 5 ?, 7.8 mm x 30.0 cm

TH-16654

Cation Guard Column OApak-P, P

TH-07166

Anion Stainless steel Silic QAE-2SW, 125 A 5 ?, 4.6 mm x 25.0 cm

TH-07157

Anion Stainless steel Polymer SAX, 5 ?, 6.0 mm x 15.0 cm

TH-07158

Cation Stainless steel Polymer SCX (H+), 5 ?, 7.8 mm x 30.0 cm

TH-07156

Cation Stainless steel Polymer SCX (NA+), 5 ?, 6.0 mm x 15.0 cm

TH-07165

Cation Stainless steel Silic SP-2SW, 125 A 5 ?, 4.6 mm x 25.0 cm

TH-14467

Cation Glass Guard Column SP-5PW

TH-07932

Cation Guard Column SP-5PW

TH-42153

Cation Guard Column SP-5PW Cartridge

TH-07211

Cation Guard Column SP-5PW Guardgel Kit

TH-08807

Cation Glass Guard Column SP-5PW Guardgel Kit

TH-16093

Cation Guard Column SP-5PW Prep Guardgel Kit

TH-18758

Cation Stainless steel Polymer SP-5PW, 1000 A 10 ?, 2.0 mm x 7.5 cm

TH-13062

Cation Glass Polymer SP-5PW, 1000 A 10 ?, 5.0 mm x 5.0 cm

TH-07161


TH-08803


TH-14017




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TH-07575


TH-07934


TH-13076

Cation Stainless steel Polymer SP-NPR, non porous, 1000 A 2.5 ?, 4.6 mm x 3.5 cm

TH-08640

Anion Stainless steel Polymer Sugar AXG, 60 A 10 ?, 4.6 mm x 15.0 cm

TH-08639

Anion Stainless steel Polymer Sugar AXI, 60 A 8 ?, 4.6 mm x 15.0 cm

TH-18257

Anion Stainless steel Polymer SuperQ-5PW , 1000 A 10 ?, 7.5 mm x 7.5 cm

TH-18387

Anion Stainless steel Polymer SuperQ-5PW , 1000 A 13 ?, 21.5 mm x 15.0 cm

TH-18389

Anion Glass Guard Column SuperQ-5PW Guardgel Kit, 20 ?

TH-18388

Anion Guard Column SuperQ-5PW Guardgel, Kit 20 ?

TH-18390

Anion Guard Column SuperQ-5PW Guardgel, Kit 20 ?

TH-18386

Anion Glass Polymer SuperQ-5PW, 1000 A 10 ?, 8.0 mm x 7.5 cm

Interacción Hidrofóbica (HIC)

The great advantage over separations by reverse phase consists in the extremely mild elution conditions which protect the stability of the most sensitive proteins. TOSO have three different columns available for HIC: TSK-GEL Phenyl-5PW. TSK-GEL Ether-5PW, and TSK-GEL Butyl -NPR.The two first have as support the packing TSK-GEL G5000PW to which have been bonded respectively phenyl or oligoethyleneglycol groups. The TSK-GEL Butyl -NPR columns are based on the same materials as the previous but with a non porous particle which is of a size of only 2.5 microns. The very high efficiencies that this column offers make it ideal for high speed applications.

Material Description ID (mm) Length (cm) μ Reference

Guard Column Ether-5PW Glass Guardgel Kit TH-14025

Guard Column Ether-5PW Guardgel Kit TH-08643

Guard Column Ether-5PW Prep Guardgel Kit TH-02424

Guard Column Phenyl-5PW Glass Guardgel Kit TH-08808

Guard Column Phenyl-5PW Guardgel Kit TH-07652

Guard Column Phenyl-5PW Prep Guardgel Kit TH-02425

Guard Column Phenyl-5PW Prep Guardgel, 5 ml 20 TH-07651

Glass Column Ether-5PW Glass, 1000Å 5 5,0 10 TH-14013


Glass Column Phenyl-5PW Glass, 1000Å 5 5,0 10 TH-13063


Stainless steel column Ether-5PW, 1000Å 2,0 7,5 10 TH-18760

Stainless steel column Ether-5PW 7,5 7,5 10 TH-08641

Stainless steel column Phenyl-5PW, 1000Å 2 7,5 10 TH-18759

Stainless steel column Phenyl-5PW 7,5 7,5 10 TH-07573



Stainless steel column Ether-5PW 21,5 15,0 13 TH-08642

Stainless steel column Phenyl-5PW 21,5 15,0 13 TH-07656

Guard Column Ether-5PW Glass Guardcolumn 20 2 13 TH-14470



tel./fax: 052/635278 | GSM: 0899/145781

Europa Peptide Reverse Phase Columns Teknokroma introduces in the market the new line of Europa HPLC columns.After the versatility of our popular mediterranea™ Sea 18 column that enables you to deal successfully with the inmense variety of separations in the fi elds of pharmaceuticals, life sciences, environment, foods, etc. Teknokroma has focused all its efforts and all its know-how, accumulated through more than 30 years of chromatographic research and development, in offering the best reverse phase HPLC packing for identifi cation and purifi cation of peptides and protein compounds. Manufactured using novel proprietary technologies, analytical and preparative Europa columns are simply the best reverse phase columns available today. As a result of these, we launch into the market the Line of Europa HPLC columns, one of the best columns in the fi eld of analysis of biomolecules. The Europa HPLC columns for peptides and proteins, provide the best performance and unsurpassed effi ciency, reliability and reproducibility. There is still a consensus that the best material to use as chromatographic packing continues to be silica. The particles of silica material are physically resistant, enable multiple functions, present maximum levels of effi ciency and are also compatible with practically all solvents. Teknokroma has dedicated years of research and development in obtaining the best silica particle on the market. The silica particle on which the Europa columns is based is the result of an optimisation process, starting with extremely pure materials with unusually low metal content, and obtaining a perfectly spherical, rigid and inert particle. Furthermore, the propietary “porifi cation process” (Surface Enhanced Accessibility, SEA) for Europa silica has achieved high surface area without sacrifi cing important properties like physical resistence and high loading capacity- making it ideal for preparative-scale processing.In addition, the Surface Enhanced Accessibility manufacturing process creates a porous structure that ensures maximum transfer speeds for solutes between the stationary and mobile phases-resulting in higher separation effi ciency. Our “Ultra-Fast” Europa columns are made in 3-5 cm length in order to get quick analytical results, whereas the “High Effi ciency” columns are normally in 15-25 cm lengths to obtain best resolution. The Teknokroma Europa Columns are uniquely designed with optimized pore size distribution; 120Å for Peptide and 300Å for the Protein Columns. Peptides: Europa C18 with 0.21, 0.30, 0.40, 0.46, 0.78, 1.0 and 2.12 cm. Proteins: Europa C18, C8 and C4 with 0.21, 0.30, 0.40, 0.46, 0.78, and 2.12 cm. Purity of silica: The responsibility for chromatographic separation of peptides and proteins is found inside the particle-within the pores. To obtain a very homogeneous pore distribution the least possible number of nanopores is essential. For most reverse-phase silica packings, these nanopores are not properly chemically bonded, endcapped or deactivated. So when nanopores are accessible to the peptides and proteins, surfacepeptide and protein interactions frequently dominate. These interactions often result in a decrease of column effi ciency.

Material Description ID (mm) Length (cm) μm Reference

Europa Peptide Sea 18 5 3 0,21 TR-010130





Europa Peptide Sea18 5 3 0,3 TR-010135








tel./fax: 052/635278 | GSM: 0899/145781



































tel./fax: 052/635278 | GSM: 0899/145781

Europa Protein Columns Reverse phase Teknokroma introduces in the market the new line of Europa HPLC columns.After the versatility of our popular mediterranea™ Sea 18 column that enables you to deal successfully with the inmense variety of separations in the fi elds of pharmaceuticals, life sciences, environment, foods, etc. Teknokroma has focused all its efforts and all its know-how, accumulated through more than 30 years of chromatographic research and development, in offering the best reverse phase HPLC packing for identifi cation and purifi cation of peptides and protein compounds. Manufactured using novel proprietary technologies, analytical and preparative Europa columns are simply the best reverse phase columns available today. As a result of these, we launch into the market the Line of Europa HPLC columns, one of the best columns in the fi eld of analysis of biomolecules. The Europa HPLC columns for peptides and proteins, provide the best performance and unsurpassed effi ciency, reliability and reproducibility. There is still a consensus that the best material to use as chromatographic packing continues to be silica. The particles of silica material are physically resistant, enable multiple functions, present maximum levels of effi ciency and are also compatible with practically all solvents. Teknokroma has dedicated years of research and development in obtaining the best silica particle on the market. The silica particle on which the Europa columns is based is the result of an optimisation process, starting with extremely pure materials with unusually low metal content, and obtaining a perfectly spherical, rigid and inert particle. Furthermore, the propietary “porifi cation process” (Surface Enhanced Accessibility, SEA) for Europa silica has achieved high surface area without sacrifi cing important properties like physical resistence and high loading capacity- making it ideal for preparative-scale processing.In addition, the Surface Enhanced Accessibility manufacturing process creates a porous structure that ensures maximum transfer speeds for solutes between the stationary and mobile phases-resulting in higher separation effi ciency. Our “Ultra-Fast” Europa columns are made in 3-5 cm length in order to get quick analytical results, whereas the “High Effi ciency” columns are normally in 15-25 cm lengths to obtain best resolution. The Teknokroma Europa Columns are uniquely designed with optimized pore size distribution; 120Å for Peptide and 300Å for the Protein Columns. Peptides: Europa C18 with 0.21, 0.30, 0.40, 0.46, 0.78, 1.0 and 2.12 cm. Proteins: Europa C18, C8 and C4 with 0.21, 0.30, 0.40, 0.46, 0.78, and 2.12 cm. Purity of silica: The responsibility for chromatographic separation of peptides and proteins is found inside the particle-within the pores. To obtain a very homogeneous pore distribution the least possible number of nanopores is essential. For most reverse-phase silica packings, these nanopores are not properly chemically bonded, endcapped or deactivated. So when nanopores are accessible to the peptides and proteins, surfacepeptide and protein interactions frequently dominate. These interactions often result in a decrease of column effi ciency.


EUROPA PROTEIN 300 Sea 4 5 3 0,21 TR-010095










EUROPA PROTEIN 300 Sea18 5 3 0,21 TR-010184



tel./fax: 052/635278 | GSM: 0899/145781












































tel./fax: 052/635278 | GSM: 0899/145781



































UROPA PROTEIN 300 Sea 4 5 10 1,00 TR-010109






UROPA PROTEIN 300 Sea 4 5 10 1,00 TR-010109



tel./fax: 052/635278 | GSM: 0899/145781


















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