Biochemicals - Isogen Life Science · Biochemicals Biochemicals w w w. u s b w e b . c o m 5 In...
Transcript of Biochemicals - Isogen Life Science · Biochemicals Biochemicals w w w. u s b w e b . c o m 5 In...
Biochemicals
Biochemicals ■ Acrylamides
■ ACS Biochemicals
■ Agaroses
■ Albumins
■ Amino Acids
■ Antibiotics
■ Buffers
■ Carbohydrates
■ Coenzymes
■ Detergents
■ Proteins
■ Stabilizers
■ Substrates
■ Sugars
■ Ultrapures
■ Vitamins
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Formulas and formula weights are provided for compounds having a specific known structure. The values used to calculate formula weights are taken from the 2001 Table of Atomic Weights, (2003) Pure Appl. Chem., 75, 1107-1122.
To simplify grade selection for customers the following grade definitions are provided. USB has selected grade names which are self explanatory, as well as those which reflect various industry standards. If any changes are noticed in a familiar grade designation for a given chemical, it is a result of a change to the grade definition rather than a change in the quality of the chemical.
USB Ultrapures are specially selected biochemicals and reagents of high quality, suitable for use in numerous biotechnology applications. These reagents meet stringent requirements, such as adherence to compendial references where applicable, have ultra-low levels of trace metal contamination, and low levels of DNase, RNase, protease and endotoxins where applicable.
Reference Constants for Nucleotides and NucleosidesValues for reference constants are taken from the literature. They are useful in determining the concentration of stock solutions prepared in the laboratory.
DBR: “Data for Biochemical Research” Dawson, R. M. C., Elliot, D. C., Elliot, W. H. , Jones, K. M., (1986) Oxford Science Publications.
NRC: “Specifications and Criteria for Biochemical Compounds,” (1972) National Academy of Sciences, Washington, D.C. 3rd edition.
Biochemical Grade DefinitionsACS Reagent Grade: Meets the limits of purity for inorganic chemicals as established by the American Chemical Society.
CP Grade (Chemically Pure): Meets the requirements of the Food Chemicals Codex, but not intended for food use.
Genetic Performance Certified™ (GPC): Functionally tested in specific molecular biology applications (e.g. nucleic acid recovery, in-gel restriction enzyme digestion or ligation) to insure optimal agarose performance.
Hi-Res™: Agaroses especially selected for high resolution of samples.
USBioAnalyzed: Meets USP or NF chemical testing requirements but is not sold as USP or NF Grade; USP and NF Grades (United States Pharmacopeia and National Formulary) must meet the requirements of those compendia and will fall under this category.
USP Grade: Meets the requirements of the U.S. Pharmacopeia.
NF Grade: Meets the requirements of the National Formulary.
MB Grade (Molecular Biology): Applies to products that are suitable for a variety of Molecular Biology applications including those which require ultra-low levels of trace metal contamination, and low levels of DNase, RNase and protease. These products are also functionally tested when applicable.
Reagent Grade: Suitable for use in general laboratory applications; meets in-house established limits in the absence of any compendial reference for the given compound. In-house established limits and their associated test methods are in many cases derived from common compendial methods such as USP, NF, ACS, etc.
High Purity Grade: Generally meets more stringent requirements than Reagent Grade products, such as higher assay limits or lower levels of trace contaminants. When available, this grade is more suitable as a reference material than the Reagent Grade.
The Biochemicals listed in this section are available in bulk quantities. Please call to inquire on sizes and pricing.
USB believes ISO 9001 certification demonstrates our commitment to quality throughout all segments of the company. Customers can be assured that the employees of USB take quality very seriously. Quality products and services are obtained through the understanding, participation and commitment of all USB employees.
A Few Notes About USB Biochemicals
Biochemicals
Biochemicals
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Ac-Co A ..........Acetyl-coenzyme A iCDH ................. Isocitrate dehydrogenaseAc-P ...............Acetyl phosphate IDP ................... Inosine-5'-diphosphateAChE ..............Acetylcholinesterase IMP ................... Inosine-5'-monophosphateADH ...............Alcohol dehydrogenase INT ................... Iodonitrotetrazolium violetADP ...............Adenosine-5'-diphosphate ITP.................... Inosine-5'-triphosphateALD ................Fructose-1, 6-diphosphate aldolase dITP ..................2'-Deoxyinosine-5'-triphosphateAIDH...............Aldehyde dehydrogenase ddITP ................2',3'-Dideoxyinosine-5'-triphosphateAMP ...............Adenonsine-5'-monophosphate LDH .................. L-Lactate dehydrogenaseD-AOD ............D-Amino acid oxidase D-LDH ...............D-Lactate dehydrogenaseL-AOD ............L-Amino acid oxidase MDH ................. L-Malate dehydrogenaseAP ..................Alkaline phosphatase MK ...................MyokinaseASOD .............Ascorbate oxidase MPI ...................Mannosephosphate isomeraseATP ................Adenosine-5'-triphosphate MUTARO............MutarotasedATP ..............2'-Deoxyadenosine-5'-triphosphate NAD+ ...............Nicotinamide-adenine dinucleotideddATP ............2',3'-Dideoxyadenosine-5'-triphosphate NADH ................Nicotinamide-adenine dinucleotide, reducedATPase ...........Adenosine-5'-triphosphate NADP+ .............Nicotinamide-adenine dinucleotide phosphateBAEE ..............Benzoyl-L-arginine ethyl ester NADPH ..............Nicotinamide-adenine dinucleotide phosphate,BAPNA ...........N—Benzoyl-L-arginine-p-nitroanilide reducedt-Boc ..............tert-butoxy carbonyl NaOAc ..............Sodium acetateBSA ................Bovine serum albumin NBT ..................Nitro-Blue-tetrazolium saltCBZ ................Carbonbenzoxy NPS ..................Nitrophenyl sulphenylChE ................Cholinesterase 2-OG(α-KG) .......2-OxoglutarateCK(CPK) ..........Creatine kinase (α-Ketoglutarate)CoA(CoA-SH) ...Coenzyme A PALP .................Pyridoxal-5-phosphateCP ..................Creatine phosphate PAMP ................Pyridoxamine-5-phosphateCTP ................Cytidine-5'-triphosphate PC ....................Paper chromatographydCTP ..............2'-Deoxycytidine-5'-triphosphate PEP ..................Phosphoenol pyruvateddCTP ............2'-3'-Dideoxycytidine-5'-triphosphate 6-PGDH ............6-Phosphogluconate dehydrogenaseDAP ...............Dihydroxyacetone phosphate 6-PG .................6- Phosphogluconate,DNA ...............Deoxyribonucleic acid (D-Gluconate-6-phosphate)DNase ............Deoxyribonuclease PGI ...................Phosphoglucose isomerase,DNP ...............Dinitrophenyl (Phosphohexose isomerase),DNPyr .............Dinitropyridyl (Glucosephosphate isomerase)DTE ................Dithioerythritol PGK ..................3-Phosphoglycerate kinaseDTT ................Dithiothreitol PGIuM ...............PhosphoglucomutaseEDTA ..............Ethylenediaminetetraacetic acid PGM .................Phosphoglycerate mutaseENOL ..............Enolase Pi ..................... Inorganic phosphateFAD ................Flavin-adenine dinucleotide PK ....................Pyruvate kinaseFDH ................Formate dehydrogenase PL-A .................Phospholipase AFDP ................D-Fructose-1,6-diphosphate PL-D .................Phospholipase DFDPase ...........Fructose diphosphatase POD ..................PeroxidaseFMN ...............Flavin mononucleotide Ppase ...............Pyrophosphatase, inorganicF-1-P .............D-Fructose-1-phosphate Ppi.................... Inorganic pyrophosphateF-6-P .............D-Fructose-6-phosphate PPO ..................2,5-Diphenyl oxazoleF-6-PK ...........Fructose-6-phosphate kinase PTA...................PhosphotransacetylaseFUM ...............Fumarase RNA ..................Ribonucleic acidGal-1-P ..........Galactose-1-phosphate tRNA ................. Transfer ribonucleic acidGAP ...............Glyceraldehyde-3-phosphate RNase ...............RibonucleaseGAPDH ...........D-Glyceraldehyde-3-phosphate dehydrogenase SDH ..................Sorbitol dehydrogenase,α-GDH ...........α-Glycerol phosphate dehydrogenase, (Polyol dehydrogenase) (Glycerol-1-phosphate dehydrogenase), SP ....................Sucrose phosphorylase (Glycerol-3-phosphate dehydrogenase) TALD ................. TransaldolaseGDP ...............Guanosine-5'-diphosphate TIM ................... Triosephosphate isomeraseGK .................Glycerokinase Tris ................... Tris-hydroxymethyl-aminomethaneGIDH ..............L-Glutamate dehydrogenase TTP (dTTP) ........ Thymidine-5'-triphosphateGMP ...............Guanosine-5'-monophosphate (2'-Deoxythymidine-5'-triphosphate)GOD ...............Glucose oxidase ddTTP ...............2',3'-dideoxythymide-5'-triphosphateGOT ...............Glucamate-oxaloacetate transaminase UDP ..................Uridine-5'-diphosphateG-1-P .............D-Glucose-1-phosphate UDPG ................Uridine-5'-diphosphate glucoseG-1,6-P
2 .........D-Glucose-1,6-diphosphate UDPgal ..............Uridine-5'-diphosphate galactose
G-6-P .............D-Glucose-6-phosphate UDPGP ..............Uridine-5'-diphosphate glucose pyrophosphorylaseG-6-PDH .........Glucose-6-phosphate dehydrogenase (Glucose-1-phosphate uridylyltransferase)GPT ................Glutamate pyruvate transaminase UMP .................Uridine-5'-monophosphateGR .................Glutathione reductase UTP ..................Uridine-5'-triphosphateGSH ...............Glutathione, reduced dUTP.................2'-Deoxyuridine-5'-triphosphateGSSG .............Glutathione, oxidized ddUTP ...............2',3'-Dideoxyuridine-5'-triphosphateγ-GT ...............γ-Glutamyltransferase XOD .................. Xanthine oxidaseGTP ................Guanosine-5'-triphosphate dGTP ..............2'-Deoxyguanosine-5'-triphosphate DBR: “Data For Biochemical Research” Dawson, R. M. C., ddGTP ............2',3'-Dideoxyguanosine-5'-triphosphate Elliot, D. C.,Elliot, W. H., Jones, K. M., Oxford Science Publications (1986) HK ..................Hexokinase NRC: “Specifications and Criteria for Biochemical Compounds.” National HPLC ..............High performance liquid chromatography Academy of Sciences, Washington, D.C., 3rd edition (1972).
Biochemical Acronyms
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Standard Atomic Weights of the Elements
Atomic Number Element Symbol Weight
89 . . . . . . . .Actinium . . . . . . . . Ac . . . . . . . . 227.0277 13 . . . . . . . .Aluminum. . . . . . . . Al . . . . . . . . . 26.981539 95 . . . . . . . .Americium . . . . . . . Am . . . . . . . 243.0614* 51 . . . . . . . .Antimony . . . . . . . . Sb . . . . . . . . 121.75 18 . . . . . . . .Argon. . . . . . . . . . . Ar . . . . . . . . . 39.948 33 . . . . . . . .Arsenic. . . . . . . . . . As . . . . . . . . . 74.92159 85 . . . . . . . .Astatine . . . . . . . . . At . . . . . . . . . 209.9871* 56 . . . . . . . .Barium . . . . . . . . . Ba . . . . . . . . 137.327 97 . . . . . . . .Berkelium . . . . . . . Bk. . . . . . . . . 247.0703* 4 . . . . . . . . .Beryllium . . . . . . . . Be . . . . . . . . . . 9.012182 83 . . . . . . . .Bismuth . . . . . . . . . Bi . . . . . . . . . 208.98037 107 . . . . . . .Bohrium . . . . . . . . . Bh . . . . . . . . 264.12 5 . . . . . . . . .Boron. . . . . . . . . . . B . . . . . . . . . . 10.811 35 . . . . . . . .Bromine . . . . . . . . Br . . . . . . . . . 79.904 48 . . . . . . . .Cadmium . . . . . . . . Cd . . . . . . . . 112.411 55 . . . . . . . .Cesium . . . . . . . . . Cs. . . . . . . . . 132.90543 20 . . . . . . . .Calcium . . . . . . . . . Ca . . . . . . . . . 40.078 98 . . . . . . . .Californium. . . . . . . Cf . . . . . . . . . 251.0796* 6 . . . . . . . . .Carbon . . . . . . . . . . C . . . . . . . . . . 12.011 58 . . . . . . . .Cerium . . . . . . . . . Ce . . . . . . . . 140.115 17 . . . . . . . .Chlorine . . . . . . . . . Cl . . . . . . . . . . 35.4527 24 . . . . . . . .Chromium . . . . . . . Cr . . . . . . . . . . 51.9961 27 . . . . . . . .Cobalt . . . . . . . . . . Co . . . . . . . . . 58.9332 29 . . . . . . . .Copper . . . . . . . . . Cu . . . . . . . . . 63.546 96 . . . . . . . .Curium. . . . . . . . . . Cm . . . . . . . . 247.0704* 105 . . . . . . .Dubnium . . . . . . . . Db . . . . . . . . 262.1141 66 . . . . . . . .Dysprosium . . . . . . Dy. . . . . . . . . 162.5 99 . . . . . . . .Einsteinium. . . . . . . Es. . . . . . . . . 252.0830 68 . . . . . . . .Erbium . . . . . . . . . Er . . . . . . . . . 167.26 63 . . . . . . . .Europium . . . . . . . . Eu. . . . . . . . . 151.965 100 . . . . . . .Fermium. . . . . . . . . Fm . . . . . . . . 257.0951 9 . . . . . . . . .Fluorine . . . . . . . . . F. . . . . . . . . . . 18.9984032 87 . . . . . . . .Francium . . . . . . . . Fr . . . . . . . . . 223.0197 64 . . . . . . . .Gadolinium . . . . . . . Gd . . . . . . . . 157.25 31 . . . . . . . .Gallium . . . . . . . . . Ga . . . . . . . . . 69.723 32 . . . . . . . .Germanium. . . . . . . Ge . . . . . . . . . 72.61 79 . . . . . . . .Gold. . . . . . . . . . . . Au . . . . . . . . 196.96654 72 . . . . . . . .Hafnium . . . . . . . . Hf . . . . . . . . . 178.49 108 . . . . . . .Hassium. . . . . . . . . Hs 2 . . . . . . . . .Helium . . . . . . . . . He . . . . . . . . . . 4.002602 67 . . . . . . . .Holmium . . . . . . . . Ho . . . . . . . . 164.93032 1 . . . . . . . . .Hydrogen . . . . . . . . H . . . . . . . . . . . 1.00794 49 . . . . . . . . Indium . . . . . . . . . . In . . . . . . . . . 114.82 53 . . . . . . . . Iodine. . . . . . . . . . . I . . . . . . . . . . 126.90447 77 . . . . . . . . Iridium . . . . . . . . . . Ir . . . . . . . . . 192.22 26 . . . . . . . . Iron . . . . . . . . . . . . Fe. . . . . . . . . . 55.847 36 . . . . . . . .Krypton . . . . . . . . . Kr . . . . . . . . . . 83.8 57 . . . . . . . .Lanthanum . . . . . . La. . . . . . . . . 138.9055 103 . . . . . . .Lawrencium . . . . . . Lr . . . . . . . . 262.1097 82 . . . . . . . .Lead . . . . . . . . . . . Pb . . . . . . . . 207.2 3 . . . . . . . . .Lithium. . . . . . . . . . Li . . . . . . . . . . . 6.941 71 . . . . . . . .Lutetium. . . . . . . . . Lu . . . . . . . . 174.967 12 . . . . . . . .Magnesium . . . . . . Mg . . . . . . . . . 24.305 25 . . . . . . . .Manganese. . . . . . . Mn . . . . . . . . . 54.93805 109 . . . . . . .Meitnerium. . . . . . . Mt . . . . . . . . 268.1388 101 . . . . . . .Mendelevium . . . . . Md . . . . . . . . 258.0984* 80 . . . . . . . .Mercury . . . . . . . . . Hg . . . . . . . . 200.59 42 . . . . . . . .Molybdenum. . . . . . Mo . . . . . . . . . 95.94 60 . . . . . . . .Neodymium . . . . . . Nd . . . . . . . . 144.24
Atomic Number Element Symbol Weight
10 . . . . . . . .Neon . . . . . . . . . . . Ne . . . . . . . . . 20.1797 93 . . . . . . . .Neptunium . . . . . . . Np . . . . . . . . 237.0482* 28 . . . . . . . .Nickel . . . . . . . . . . Ni . . . . . . . . . . 58.69 41 . . . . . . . .Niobium . . . . . . . . . Nb . . . . . . . . . 92.90638 7 . . . . . . . . .Nitrogen . . . . . . . . . N . . . . . . . . . . 14.00674 102 . . . . . . .Nobelium . . . . . . . . No . . . . . . . . 259.1010 76 . . . . . . . .Osmium . . . . . . . . . Os . . . . . . . . 190.2 8 . . . . . . . . .Oxygen. . . . . . . . . . O . . . . . . . . . . 15.9994 46 . . . . . . . .Palladium . . . . . . . . Pd . . . . . . . . 106.42 15 . . . . . . . .Phosphorus . . . . . . P . . . . . . . . . . 30.973762 78 . . . . . . . .Platinum. . . . . . . . . Pt . . . . . . . . . 195.08 94 . . . . . . . .Plutonium. . . . . . . . Pu . . . . . . . . 244.0642* 84 . . . . . . . .Polonium . . . . . . . . Po . . . . . . . . 208.9824* 19 . . . . . . . .Potassium . . . . . . . K . . . . . . . . . . 39.0983 59 . . . . . . . .Praseodymium . . . . Pr . . . . . . . . . 140.90765 61 . . . . . . . .Promethium . . . . . . Pm . . . . . . . . 144.9127* 91 . . . . . . . .Protactinium . . . . . . Pa. . . . . . . . . 231.0359 88 . . . . . . . .Radium . . . . . . . . . Ra . . . . . . . . 226.0254* 86 . . . . . . . .Radon . . . . . . . . . . Rn . . . . . . . . 222.0176* 75 . . . . . . . .Rhenium. . . . . . . . . Re . . . . . . . . 186.207 45 . . . . . . . .Rhodium. . . . . . . . . Rh . . . . . . . . 102.9055 37 . . . . . . . .Rubidium . . . . . . . . Rb . . . . . . . . . 85.4678 44 . . . . . . . .Ruthenium . . . . . . . Ru . . . . . . . . 101.07 104 . . . . . . .Rutherfordium. . . . . Rf . . . . . . . . . 261.1088 62 . . . . . . . .Samarium. . . . . . . . Sm . . . . . . . . 150.36 21 . . . . . . . .Scandium. . . . . . . . Sc. . . . . . . . . . 44.95591 106 . . . . . . .Seaborgium . . . . . . Sg . . . . . . . . 266.1219 34 . . . . . . . .Selenium . . . . . . . . Se. . . . . . . . . . 78.96 14 . . . . . . . .Silicon . . . . . . . . . . Si . . . . . . . . . . 28.0855 47 . . . . . . . .Silver . . . . . . . . . . . Ag . . . . . . . . 107.8682 11 . . . . . . . .Sodium . . . . . . . . . Na . . . . . . . . . 22.989768 38 . . . . . . . .Strontium . . . . . . . . Sr . . . . . . . . . 87.62 16 . . . . . . . .Sulfur. . . . . . . . . . . S . . . . . . . . . . 32.066 73 . . . . . . . .Tantalum . . . . . . . . Ta . . . . . . . . . 180.9479 43 . . . . . . . .Technetium. . . . . . . Tc. . . . . . . . . . 97.9072* 52 . . . . . . . .Tellurium . . . . . . . . Te . . . . . . . . . 127.6 65 . . . . . . . .Terbium . . . . . . . . . Tb. . . . . . . . . 158.92534 81 . . . . . . . .Thallium . . . . . . . . . Tl . . . . . . . . . 204.3833 90 . . . . . . . .Thorium . . . . . . . . . Th. . . . . . . . . 232.0381* 69 . . . . . . . .Thulium . . . . . . . . . Tm . . . . . . . . 168.93421 50 . . . . . . . .Tin . . . . . . . . . . . . . Sn . . . . . . . . 118.71 22 . . . . . . . .Titanium. . . . . . . . . Ti . . . . . . . . . . 47.88 74 . . . . . . . .Tungsten . . . . . . . . W . . . . . . . . . 183.85 112 . . . . . . .Ununbium. . . . . . . . Uub 116 . . . . . . .Ununhexium . . . . . . Uuh 110 . . . . . . .Ununnilium . . . . . . . Uun 114 . . . . . . .Ununquadium . . . . . Uuq 111 . . . . . . .Unununium. . . . . . . Uuu. . . . . . . . 272.1535 92 . . . . . . . .Uranium . . . . . . . . . U . . . . . . . . . 238.0508* 23 . . . . . . . .Vanadium . . . . . . . . V . . . . . . . . . . 50.9415 54 . . . . . . . .Xenon . . . . . . . . . . Xe. . . . . . . . . 131.29 70 . . . . . . . .Ytterbium . . . . . . . . Yb. . . . . . . . . 173.04 39 . . . . . . . .Yttrium . . . . . . . . . . Y . . . . . . . . . . 88.90585 30 . . . . . . . .Zinc . . . . . . . . . . . . Zn. . . . . . . . . . 65.39 40 . . . . . . . .Zirconium. . . . . . . . Zr . . . . . . . . . . 91.224
*Relative atomic mass of the isotope of that element of longest known half-life.Names of individual elements with atomic numbers 110 to 116 are provisional.Based on 2001 IUPAC Table of Standard Atomic Weights of the Elements.Reproduced from Pure Appl. Chem., (2003) Vol. 75, No. 8, pp. 1107-1122 by permission of the copyright owner, IUPAC. ©2003 IUPAC.
Biochemicals
Biochemicals
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In 1966, Good, et al. introduced a series of new biological buffers for use in the physiological pH range(1). Prior to that time only a few buffer systems, consisting of weak acid/salt or weak base/salt were available to biological laboratories. Most of these systems had severe limitations associated with them and are discussed later. The “Good” zwitterionic buffers were designed to meet a genuine need, particularly in the developing field of cellular biology, and have gained rapid acceptance. Zwitterionic buffers, by definition, contain both positive and negative ionizable groups. Secondary and tertiary amines provide the positive charges, while sulfonic and carboxylic acid groups provide the negative charges.
There are very few traditional materials which are weak acids or weak bases with pK
a values effective between pH 6.0 and 9.0 where most
biological processes take place. All of these compounds exhibit some significant deviations from the requirements of the “ideal” buffer.
Phosphate is an active participant in many biochemical processes, being an activator, inhibitor and/or a metabolite in many biological systems. Borate buffers complex with a wide variety of important respiratory metabolites and other organic compounds as well. For example, polyols, including carbohydrates and their derivatives, can significantly lower the pH of borate buffers through complex formation. Phosphates also demonstrate complexing capabilities with divalent and polyvalent cations and can therefore inhibit a series of metal ion-dependent biochemical reactions.
Tris (also a popular traditional biochemical buffer) is a poor buffer below pH 7.5 and is a potentially reactive primary amine which often acts as an inhibitor. It has an appreciable solubility in organic solvents which permits it to penetrate biological membranes and it has a very high pH temperature coefficient.
Bicarbonate is a poor buffer due to the limited solubility of carbon dioxide and the associated problems of liquid/gas equilibrium. Since bicarbonate buffers spontaneously liberate carbon dioxide (equation 1) they require sealed vessels capable of maintaining a carbon dioxide atmosphere in order to sustain a constant pH.
1. CO3
= + 2H
+ HCO
3
- + H
+ H
2O+CO
2
Bicarbonate’s pKa is 6.3 and therefore has a greatly diminished buffering
capacity within the physiologically important pH range of 7 to 7.5.
From the Henderson-Hassellbach equation, (equation 2), it can be shown that optimal buffering occurs when the working pH is equal to the pK
a of
the ionizing group, and falls at an increasing distance from that value. In practice, the useful pH range for a buffer is its pK
a ± 1 pH unit.
2. pH = pKa + log [(activity of base) ÷ (activity of acid)]
Good’s buffers were designed to meet the following criteria of an effective “ideal” biological buffer as much as possible:
• pKa between 6.0 and 8.0, the region of most biological reactions
• High aqueous solubility• Minimal penetration of biological membranes• Minimal salt effects or effects due to the ionic composition of the solution• Minimal effects due to concentration or temperature• Minimal interactions with mineral cations• Enzymatic and hydrolytic stability• Minimal absorbance between 240 and 700 nm• Easy to purify• Non-toxic• Minimal participation in biochemical reactions
Helpful Suggestions1. When preparing concentrated solutions refer to solubility data.2. Media that contain buffers may be adjusted to correct pH with NaOH
or HCl. If this is not feasible, the buffer stock can be adjusted to correct pH before addition to the media.
3. Solutions containing buffers can be sterilized by membrane filtration or normal autoclaving procedures.
4. Don’t autoclave solutions which have been adjusted with Sodium Bicarbonate.
5. PIPES requires the addition of alkali to effect solution.6. Always use pyrogen free distilled water when preparing media from
dry chemicals.7. Always choose a buffer with a pK
a close to the pH that is required. If
the pH is expected to drop during the project select a buffer with a pKa
slightly lower than the pH.8. Always adjust the pH of the crystalline buffers when placed into
solution, otherwise the pH will be incorrect in most instances.9. Use tetramethylammonium hydroxide when a buffer without mineral
cations is required.10. Whenever possible prepare the buffer solution at the concentration
and temperature of the experimental conditions under which it will be used.
11. Other buffers suitable for ultraviolet spectroscopy include many zwitterionic acids. For example, at concentrations of 0.05M, BES, Bicine, HEPES, MES, PIPES,TES and Tricine have negligible absorption above 240 nm and can be used successfully at 230 nm. ACES absorbs significantly at 230 nm and ADA is limited to above about 260 nm.
References:1. Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S. and
Singh, R. M. M. (1966) Biochemistry 5, 467-477.2. Schwarzenbach, G. (1955) Helv. Chim. Acta. 38, 1147.3. Good, N. E. and Izawa, S. (1972) Methods Enzymol. 24, 53-68.
Good’s Buffers Guidelines
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sBiochemicals
6 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
∆ pKa/°C Molarity of Common Formula Temperature Saturated Name Formula Weight Coefficient Solution at 0°C pKa (4°C) pKa (20°C) pKa (37°C)
ACES H2NCOCH
2NH 182.20 -0.020 0.22 7.20 6.90 6.54
CH2 CH
2SO
3H
ADA NH2COCH
2N 190.15 -0.011 0.09 6.80 6.60 6.43
(CH2 COOH)
2
BES (HOCH2CH
2)2NCH
2 213.25 -0.016 3.2 7.41 7.15 6.88
CH2 SO
3H
Bicine (CH2CH
2OH)
2NCH
2 163.17 -0.018 1.1 8.64 8.35 8.04
COOH
CAPS (C6H
11)NH(CH
2)3 221.32 -0.021 0.47 10.74 10.40 10.04
SO3H
CHES (C6H
11)NH(CH
2)2 207.29 -0.011 1.14 9.73 9.55 9.36
SO3H
Glycinamide NH2CH
2CONHCH
2 132.12 -0.028 1.1 8.85 8.40 7.92
COOH
Glycinamide•HCl H2NCH
2CONH
2• 110.54 -0.029 4.6 8.66 8.20 7.71
HCl
HEPES HO(CH2)2(C
4H
8N
2) 238.30 -0.014 2.25 7.77 7.55 7.31
(CH2)2SO
3H
HEPPS HO3S(CH
2)3 252.33 -0.011 1.58 8.18 8.00 7.81
(C4H
8N
2)(CH
2)2OH
MES HO3S(CH
2)2 213.25 -0.011 0.65 6.33 6.15 5.96
(C4H
8NO)•H
2O
MOPS HO3S(CH
2)3 209.26 -0.011 3.09 7.38 7.20 7.01
(C4H
8NO)
PIPES HO3S(CH
2)2(C
4H
8N
2) 302.37 -0.0085
_____ 6.94 6.80 6.66
(CH2)2SO
3H
TES (HOCH2)3CNHCH
2 229.25 -0.020 2.6 7.82 7.50 7.16
CH2SO
3H
TRICINE (CH2OH)
3CNHCH
2 179.17 -0.021 0.8 8.49 8.15 7.79
COOH
TRIS NH2C(CH
2OH)
3 121.14 -0.031 2.4 8.80 8.30 7.77
References:1. Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S. and Singh, R. M. M. (1966) Biochemistry 5, 467-477.2. Schwarzenbach, G. (1955) Helv. Chim. Acta. 38, 1147.3. Good, N. E. and Izawa, S. (1972) Methods Enzymol. 24, 53-68.
Physical Properties of Good’s and Other Biological Buffers
Biochemicals
Biochemicals
7w w w . u s b w e b . c o m
ACESH
2NCOCH
2NHCH
2CH
2SO
3H
Formula Weight: 182.20PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 6.90 ± 0.15A (260 nm, 5%, 1 cm): ≤ 0.1Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.2%Sulfate (SO
4): ≤ 0.01%
Iron (Fe): ≤ 5 ppmLead (Pb): ≤ 10 ppmCAS #7365-82-4
10001 10gm 25gm 100gm 1kg
AcetamideC
2H
5NO
Formula Weight: 59.07PRODUCT SPECIFICATIONSForm: White/clear crystalsAssay: ≥ 99%Identity: By IRMelting Point: 77 - 82°CMoisture (KF): ≤ 1.0%CAS #60-35-5
10000 50gm 100gm
AcetophenetidineC
10H
13NO
2
Formula Weight: 179.22CAS #62-44-2
10035 200gm 500gm 1kg
S-Acetyl Coenzyme A, Lithium Salt
ULTRAPUREC
23H
35N
7O
17P
3SLi
3•3H
2O
Formula Weight: 881.42PRODUCT SPECIFICATIONSForm: White powderAssay (Total UV, pH 7.0): ≥ 90%λ
max: 259 ± 1 nm
εmax
(pH 7): [14.6 ± 0.8] x 103
Spectral Ratios: A
232/A
260: 0.51 ± 0.02
A250
/A260
: 0.86 ± 0.04 A
280/A
260: 0.17 ± 0.02
Storage: -20°CCAS #75520-41-1
10062 10mg 25mg 100mg 500mg
N-Acetyl-L-CysteineHSCH
2CH(NHCOCH
3)COOH
Formula Weight: 163.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 102.0% C
5H
9NO
3S (dry basis)
Identity: By IRLoss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.5%Heavy Metals (Pb): ≤ 10 ppmCAS #616-91-1
10120 10gm 50gm 100gm 500gm 1kg
N-Acetyl-D-Galactosamine(N-Acetylchondrosamine)
C8H
15NO
6
Formula Weight: 221.21PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97%[α]20
D : +83° ± 5° (c=1, H
2O)
Storage: 4°CCAS #14215-68-0
10140 100mg 500mg 1gm 5gm
N-Acetyl-D-GlucosamineC
8H
15NO
6
Formula Weight: 221.21PRODUCT SPECIFICATIONSForm: White to off-white powderSolubility (50 mg/ml): Clear to very slightly hazy;
Colorless to yellow solution in H2O
[α]20D : +40.9° ± 2° (c=2 in H
2O)
Loss on Drying: ≤ 0.1%Heavy Metals: ≤ 10 ppmCAS #7512-17-6
10145 10gm 25gm 100gm 500gm
N-Acetyl-DL-Phenylalanine-p-Nitroanilide
C17
H17
N3O
4
Formula Weight: 327.33Storage: 4°CCAS #19746-42-0
10240 250mg 1gm 5gm 10gm
Acetylated BSASee Albumin, Bovine, Acetylated, page 187
Acid Phosphatase(E.C.3.1.3.2)Source: Wheat GermForm: Lyophilized powderUnit Definition: One unit hydrolyzes 1 µmol of
o-carboxyphenylphosphate/min at 25°C, pH 5.0.
Storage: 4°CCAS #9001-77-8
10380 1gm
Bioc
hem
ical
sBiochemicals
8 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Acridine Orange, Zinc Chloride Double Salt
(C.I.46005)C
17H
19N
3•HCl•1/
2ZnCl
2
Formula Weight: 369.97PRODUCT SPECIFICATIONSForm: Maroon/rust colored powderIdentity: By IRApplication: •Used to distinguish single-stranded from
double-stranded nucleic acidsTypical Procedure:1. Stain the agarose gel with a solution of 30 µg/
ml acridine orange in 10mM sodium phosphate monohydrate, pH 7.0, for 15 minutes at room temperature in the dark.
2. Destain by running hot tap water over the gel for 15-20 minutes.
3. Visualize bands by UV illumination with a 300 nm light source.
4. Single-stranded DNA fluoresces orange-red. Double-stranded DNA fluoresces green.
CAS #10127-02-3
10390 5gm 25gm 100gm
Acriflavin NeutralC
14H
14ClN
3
Formula Weight: 259.73PRODUCT SPECIFICATIONSForm: Orange, granular powderIdentity: By IRLoss on Drying: ≤ 8%Arsenic (As): ≤ 10 ppmChlorine: 13.0 - 16.0%CAS #8048-52-0
10395 25gm 100gm
Acrylamide
ULTRAPUREMB GradeCH
2=CHCONH
2
Formula Weight: 71.08PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 99.9%Identity: By IRA (410 nm, 35%, 1 cm): ≤ 0.02A (290 nm, 1%, 1 cm): ≤ 0.1A (280 nm, 7mM, 1 cm): ≤ 0.04A (252 nm, 7mM, 1 cm): ≤ 1.20Insoluble Matter (H
2O): ≤ 0.005%
Insoluble Matter (MeOH): ≤ 0.005%pH (10%, 0.1N NaCl): 5.5 - 7.0Melting Point: 84° ± 1°CAcrylic Acid: ≤ 0.001%Conductivity (35%): ≤ 5 µmhos/cmIron (Fe): 1 ppmLead (Pb): ≤ 1 ppmGel Performance: Passes testApplications:•Basic component for preparing polyacrylamide
gels•Used at a 19:1 ratio with bis-acrylamideDNA Size Separation by Acrylamide Percentage: DNA Size Range Concentration of (Base Pairs) Acrylamide (%) 100 - 1,000 3.5 80 - 500 5.0 60 - 400 8.0 40 - 200 12.0 10 - 100 20.0CAS #79-06-1
75820 25gm 100gm 500gm 1kg
AcrylamideReagent GradeC
3H
5NO
Formula Weight: 71.08PRODUCT SPECIFICATIONSForm: White, clear crystalline powderAssay: ≥ 99.95%Identity: By IRA (290 nm, 1%, 1 cm): ≤ 0.125pH (1%, H
2O): 5.8 - 7.5
Acrylic Acid Content: ≤ 0.001%Insoluble Matter: ≤ 0.005%CAS #79-06-1
32800 25gm 100gm 500gm 1kg
Acrylamide Gel Mixes and SolutionsDNA Sequencing Gel Mix, TBE Buffer, 6%,
page 222Glycerol Tolerant Gel (GTG) Buffer, 20X Solution,
page 234Glycerol Tolerant Gel (GTG) Buffer, 20X Ready-
Mixed Powder, page 234RapidGel™ 40% Liquid Acrylamide Stock
Solution, page 281RapidGel DNA Sequencing Gel Solution, TBE
Buffer, 4%, page 281RapidGel DNA Sequencing Gel Solution, TBE
Buffer, 6%, page 281RapidGel DNA Sequencing Gel Solution, TBE
Buffer, 8%, page 281RapidGel-XL 6% DNA Sequencing Gel Solution,
page 282RapidGel-XL 40% Liquid Acrylamide Stock
Solution, page 282Stop Solution, page 291
Actinomycin D
ULTRAPURESource: Streptomyces antibioticusC
62H
86N
12O
16
Formula Weight: 1255.42PRODUCT SPECIFICATIONSForm: Bright red powderAssay (A
441, MeOH): ≥ 93.0%
Application:•Actinomycin D is a specific inhibitor of DNA-
dependent RNA synthesis and is used in the preparation of cDNA. The concentration of a solution of actinomycin can be found according to Beer’s Law, by measuring its absorbance at 441 nm, based on a molar extinction coefficient of 21,900 (in water) and a molecular weight of 1255.
Thus: c = ε/Ab, where: c = the concentration in molarity of the
solution ε = the molar extinction coefficient (21,900) A = the absorbance of the solution b = the path length of the cellSolubility: 10 mg/ml in ethanol or DMSONote: Actinomycin D is toxic and light sensitive.
Gloves should be worn when handling. Store the stock solution (10 mg/ml) in a foil-wrapped vial at -20°C.
Reference:1. Berger, S. L. and Kimmel, A. R. (1987), “Guide
to Molecular Cloning,” Academic Press, Inc., 320.
Storage: 4°CCAS #50-76-0
10415 2mg 5mg 25mg
Biochemicals
Biochemicals
9w w w . u s b w e b . c o m
ADANH
2COCH
2N(CH
2COOH)
2
Formula Weight: 190.15PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 6.60 ± 0.15A (280 nm, 0.1M in 1N NaOH,1 cm): ≤ 0.1Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #26239-55-4
10421 100gm 1kg
AdenineC
5H
5N
5
Formula Weight: 135.13PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderIdentity: By IRAssay: ≥ 98.0%Loss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.1%Sulfate (SO
4): ≤ 0.028%
Heavy Metals (Pb): ≤ 20 ppmArsenic (As): ≤ 2 ppmReference Constants:λ
max (pH 7, NRC ): 261 nm
εmax
(pH 7, NRC ): 13.4 x 103
ε260
(pH 7, DBR ): 13.3 x 103
CAS #73-24-5
10430 5gm 25gm 100gm 1kg
Adenine, Hydrochloride(6-Aminopurine)
C5H
5N
5•HCl
Formula Weight: 171.59 (anhydrous) 180.60 (hemihydrate*)PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderAssay (By UV): ≥ 98.0% (dry basis)Identity: By IRChromatography (TLC): HomogeneousMoisture (KF): ≤ 6.0%*Note: The moisture content of this product
varies from anhydrous to slightly above the theoretical hemihydrate.
CAS #6055-72-7
10440 5gm 10gm 25gm 100gm
Adenine Sulfate, Dihydrate(C
5H
5N
5)2•H
2SO
4•2H
2O
Formula Weight: 404.36PRODUCT SPECIFICATIONSForm: White to pale-yellow crystalline powderAssay (Total UV): ≥ 97.0%Identity: By IRSpectral Ratios (pH 1.0): A
250/A
260: 0.76 ± 0.04
A280
/A260
: 0.38 ± 0.04Moisture (KF): ≤ 10%CAS #321-30-2
10450 5gm 10gm 25gm 100gm 1kg
AdenosineC
10H
13N
5O
4
Formula Weight: 267.24PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV): ≥ 98.0%Chromatography (TLC): Homogeneousλ
max: 257 nm ± 2 nm
Spectral Ratios (0.1N HCl): A
250/A
260: 0.85 ± 0.03
A280
/A260
: 0.21 ± 0.02Loss on Drying: ≤ 0.5%Reference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 6, DBR ): 14.9 x 103
CAS #58-61-7
10460 5gm 10gm 25gm 100gm
Adenosine-3'-5'-Cyclic Monophosphate
C10
H12
N5O
6P
Formula Weight: 329.21PRODUCT SPECIFICATIONSForm: White powderPurity: ≥ 98% (dry basis)Moisture (KF): ≤ 10%Storage: 4°CReference Constants:λ
max (pH 7, NRC ): 258 nm
εmax
(pH 7, NRC ): 14.7 x 103
CAS #60-92-4
10470 100mg 1gm 5gm
ADPSee Adenosine-5'-Diphosphate, Disodium Salt,
Dihydrate, page 173
Adenosine-3'-5' Cyclic Monophosphate, Sodium Salt
C10
H11
N5O
6PNa
Formula Weight: 351.19PRODUCT SPECIFICATIONSForm: White powderAssay (UV, pH 2.0): ≥ 98% (dry basis)Storage: 4°CReference Constants:λ
max (pH 7, NRC ): 258 nm
εmax
(pH 7, NRC ): 14.7 x 103
CAS #37839-81-9
10472 100mg 1gm 5gm
Adenosine-5'-Diphosphate, Disodium Salt, Dihydrate(ADP)
C10
H13
N5O
10P
2Na
2•2H
2O
Formula Weight: 507.20PRODUCT SPECIFICATIONSForm: White powderAssay (Total UV): 100 ± 4%Spectral Ratios: A
250/A
260: 0.78 ± 0.03
A280
/A260
: 0.16 ± 0.02Storage: -20°C in air-tight containers.Reference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 7, DBR ): 15.4 x 103
CAS #16178-48-6
10490 500mg 1gm 5gm 10gm 25gm
Adenosine-5'-Diphosphate, Trisodium Salt(ADP)
C10
H12
N5O
10P
2Na
3•nH
2O
Formula Weight: 493.15•nH2O
Storage: -20°CCAS #2092-65-1
10500 500mg 1gm 5gm
Bioc
hem
ical
sBiochemicals
10 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Adenosine-3'-Monophosphate, Hydrate(3'-AMP)
C10
H14
N5O
7P•nH
2O
Formula Weight: 347.22 (anhydrous)Reference Constants:λ
max (pH 7, DBR ): 259 nm
εmax
(pH 7, DBR ): 15.4 x 103
ε260
(pH 7, DBR ): 15.3 x 103
ε260
(pH 7, NRC ): 15.0 x 103
Storage: -20°CCAS #84-21-9
10555 250mg 500mg
Adenosine-5'-Monophosphate, Free Acid
C10
H14
N5O
7P
Formula Weight: 347.22 (anhydrous)* 365.24 (monohydrate)*PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV, pH 7.0 or 0.01N HCl): 99 ± 3%Identity: By IRSpectral Ratios (pH 7.0): A
250/A
260: 0.78 ± 0.03
A280
/A260
: 0.16 ± 0.02Spectral Ratios (0.01N HCl): A
250/A
260: 0.84 ± 0.03
A280
/A260
: 0.22 ± 0.02Moisture: ≤ 6%*Note: Moisture content may vary from anhydrous
to a monohydrate.Reference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 7, DBR ): 15.3 x 103
CAS #18422-05-4
10520 5gm 10gm 25gm 100gm
Adenosine-5'-Monophosphate, Free Acid, Monohydrate(AMP)
C10
H14
N5O
7P•H
2O
Formula Weight: 365.24PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Total UV, 0.1N HCl): ≥ 96%λ
max: 257 nm ± 2 nm
Spectral Ratios (0.1N HCl): A
250/A
260: 0.84 ± 0.03
A280
/A260
: 0.22 ± 0.02Loss on Drying: ≤ 6.0%Reference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 7, DBR ): 15.3 x 103
CAS #18422-05-4
10521 5gm 25gm 100gm 1kg
Adenosine-5'-Monophosphate, Disodium Salt(AMP)
C10
H12
N5O
7PNa
2•nH
2O
Formula Weight: 391.19•nH2O
PRODUCT SPECIFICATIONSForm: White powderAssay (UV, 0.01N HCl): 98 ± 3%λ
max: 257 nm ± 2 nm (0.01N HCl)
Spectral Ratios (0.01N HCl): A
250/A
260: 0.84 ± 0.03
A280
/A260
: 0.22 ± 0.02Heavy Metals: ≤ 10 ppmReference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 7, DBR ): 15.3 x 103
CAS #4578-31-8
10620 5gm 10gm 25gm 100gm
Adenosine-5'-Triphosphate, 100mM Solution(ATP)
ULTRAPUREC
10H
12N
5O
13P
3Na
4
Formula Weight: 595.11Form: 100mM aqueous solution, pH 7.5Storage: -20°C
77241 25µmol(250µl)
Adenosine-5'-Triphosphate, Disodium Salt(ATP)
C10
H14
N5O
13P
3Na
2•3H
2O
Formula Weight: 605.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV, pH 7 or 0.01N HCl): 100 ± 2%λ
max: 259 nm ± 2 nm (pH 7.0)
εmax
(pH 7.0): [15.4 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.80 ± 0.03
A280
/A260
: 0.15 ± 0.02 orSpectral Ratios (0.01N HCl): A
250/A
260: 0.85 ± 0.05
A280
/A260
: 0.22 ± 0.03Moisture: ≤ 15%Reference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 7, DBR ): 15.4 x 103
Storage: -20°C, protect from moisture.CAS #51963-61-2
10585 5gm 10gm 25gm 100gm 500gm
Adenosine-5'-Triphosphate, Disodium Salt, Low Iron(ATP)
C10
H14
N5O
13P
3Na
2•3H
2O
Formula Weight: 605.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV, pH 7.0 or 0.01N HCl): 100 ± 2%λ
max (pH 7.0): 259 ± 2 nm
εmax
(pH 7.0): [15.4 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.80 ± 0.03
A280
/A260
: 0.15 ± 0.02Spectral Ratios (0.01N HCl): A
250/A
260: 0.85 ± 0.05
A280
/A260
: 0.22 ± 0.03Moisture: ≤ 15%Iron (Fe): ≤ 15 ppmReference Constants:λ
max (pH 7, NRC ): 259 nm
εmax
(pH 7, NRC ): 15.4 x 103
ε260
(pH 7, DBR ): 15.4 x 103
Storage: -20°C, protect from moisture.CAS #51963-61-2
10586 1gm 5gm 10gm 25gm 100gm
Biochemicals
Biochemicals
11w w w . u s b w e b . c o m
S-Adenosyl-L-Methionine Sulfate p-Toluenesulfonate(SAMe-PTS)
C15
H23
N6O
5S(+) n HSO
4(-) n H
2SO
4C
7H
7SO
3H
Formula Weight: 766.80PRODUCT SPECIFICATIONSForm: White to off white powderAssay (HPLC): ≥ 95% (dry basis)Moisture (KF): ≤ 2.0%Storage: 4°CCAS #97540-22-2
10601 25mg 100mg
Adipic AcidHOOC(CH
2)4COOH
Formula Weight: 146.14CAS #124-04-9
10625 500gm
Adonitol(D-Ribitol)
C5H
12O
5
Formula Weight: 152.15PRODUCT SPECIFICATIONSForm: White to off-white powderMelting Point: 103° ± 2°CLoss on Drying: ≤ 0.5%Heavy Metals (Pb): ≤ 10 ppmCAS #488-81-3
10635 5gm 25gm 100gm
AEBSF Hydrochloride(4-(2-Aminoethyl)-benzenesulfonyl fluoride hydrochloride)
C8H
10FNO
2S•HCl
Formula Weight: 239.69PRODUCT SPECIFICATIONSForm: Fine white powderAssay (HPLC): ≥ 98%Identity: By IRMelting Point: 181 - 185°CSolubility (H
2O, 20°C): 10 gm/L
Storage: 4°CCAS #30827-99-7Applications:•Inactivation of trypsin and thrombin(1)
•Synthetic and low molecular weight inhibitor of serum kallikrein(2)
•Specific and irreversible inhibitor of serine proteases(3)
Notes: AEBSF irreversibly inactivates thrombin and other serine proteases (e.g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin) by sulfonylation of a functional group in the active center of the enzyme(1). AEBSF (LD
50
determined from the oral dose in mouse is 2.8 gm/kg, which is higher than those for DFP and PMSF) is a substantially less toxic substitute for PMSF and DFP. It is highly water soluble and has an acidic pH stability. AEBSF is inactivated at 37°C by 50% after 5 hours. We recommend a concentration of stock solution of 20mM or 100mM in water or recommended buffer at acidic pH. Stock solutions are stable for up to 2 months at -20°C and for approximately one week at 4°C. AEBSF should be added to an assay buffer just before use at a final concentration of 0.1 - 2mM(3-5).
References:1. Walsmann, P., Richter, M. and Markwardt, F.
(1972) Acta Biol. Med. Ger. 28, 577-585.2. Markwardt, F., Drawert, J. and Walsmann, P.
(1974) Biochem Pharmacol. 23, 2247-2256.3. Taylor, J. A., Karas, J. L., Ram, M. K., Green, O.
M. and Seidel-Dugan, C. (1995) Mol. Cell. Biol. 8, 4149-4157.
4. Murphy, B. J., Rossie, S., De Jongh, K. S. and Catterall, W. A. (1993) J. Biol. Chem. 268, 27355-27362.
5. Nathan, D. F. and Lindquist, S. (1995) Mol. Cell. Biol. 15, 3917-3925.
11118 200mg 1gm
Agar, Bacteriological
ULTRAPUREType APRODUCT SPECIFICATIONSForm: White to cream-colored powderMoisture: ≤ 10%Ash: ≤ 6.5%Gel Strength (1.5%): 550 - 950 gm/cm2
Gel pH (1.5%) after autoclaving: 6.0 - 7.5Gel Point (1.5%): 32 - 38°CMelting Point (1.5%): 80 - 90°CApplication:•Used extensively as a gelling agent in the
preparation of bacteriological culture media. Its main attributes are superior transparency, very reliable reproducibility and absence of inhibitors capable of hindering optimal development of microorganisms. Extraneous matter reduced to a minimum.
CAS #9002-18-0
10906 4oz 1lb 5lb 25lb
Agar, Noble Agar
ULTRAPUREPRODUCT SPECIFICATIONSForm: Pale cream powderMoisture: ≤ 10%Ash: ≤ 1.6%Gel Strength (1.5%): ≥ 700 gm/cm2
Gel pH (1.5%): 5.0 - 7.0Gel Point (1.5%): 32 - 37.5°CMelting Point (1.5%): 80 - 95°CA (430 nm, 1.5%): ≤ 0.150Resistance (1% suspension): ≥ 25,000 OhmsEEO (-m
r): ≤ 0.450 (pH 8.4)
Application:•Gelling agent essentially free of impurities.
Used mainly in biochemistry and molecular biology procedures wherever increased purity is required.
Typical Agar Preparation: Plates: 15 gm/l of media; 30-35 ml per
90 mm plate Top Agar: 7 gm/l of media Autoclave for 20 minutes and swirl gently to
evenly distribute the agar. If antibiotics are to be added, allow the agar to cool to 50°C. When pouring plates, gently swirl the medium. A Bunsen burner flame can be passed over the surface of the plates to remove any air bubbles. After the medium has hardened, invert the plates and store at 4°C.
CAS #9002-18-0
10907 100gm 500gm 1kg
AgarPRODUCT SPECIFICATIONSForm: Light tan granular powderIdentity: Passes testLoss on Drying: ≤ 20.0%Total Ash: ≤ 6.5%CAS #9002-18-0
10654 4oz 1lb 5lb 25lb
Agar, FlakesCAS #9002-18-0
10762
Bioc
hem
ical
sBiochemicals
1� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
USB Agarose Selection Guide
Genetic Performance Certified™ (GPC): Functionally tested in specific molecular biology applications (e.g. nucleic acid recovery, in-gel restriction enzyme digestion or ligation) to ensure optimal agarose performance.
Hi-Res™: High resolution.
Biochemicals
Biochemicals
13w w w . u s b w e b . c o m
Effect of Agarose Concentration on DNA Separation
1X TAE Buffer Gel Concentration 1X TBE Buffer
Size Range (bp) % Size Range (bp)
Agarose - Separation ≥ 500 bp, Genetic Performance CertifiedTM [75817]
20,000 - 1,000 0.6 15,000 - 1,000 12,000 - 500 0.8 10,000 - 500 8,000 - 300 1.0 7,000 - 250 6,000 - 200 1.2 5,000 - 200 3,500 - 100 1.5 3,000 - 100 2,000 - 50 2.0 2,000 - 50
Agarose - Pulse Field (PFGE) [32901]
40,000 - 3,000 0.3 20,000 - 2,000 22,000 - 2,000 0.5 12,000 - 1,500 15,000 - 1,000 0.8 9,000 - 1,000 10,000 - 500 1.0 6,000 - 500 5,000 - 200 1.8 3,000 - 200
Agarose - Hi-ResTM, Separation ≤ 1000 bp [10132]
1,500 - 100 2.0 1,200 - 100 1,000 - 50 3.0 700 - 40 500 - 20 4.0 200 - 20 300 - 10 5.0 < 100
Agarose - Hi-ResTM, Separation ≤ 1500 bp [10138]
2,500 - 700 2.0 1,500 - 500 1,200 - 500 3.0 800 - 100 700 - 100 4.0 500 - 50 250 - 30 5.0 250 - 20
Agarose - LE [32802]
20,000 - 1,000 0.6 15,000 - 1,000 12,000 - 500 0.8 10,000 - 500 8,000 - 300 1.0 7,000 - 250 6,000 - 200 1.2 5,000 - 200 3,500 - 100 1.5 3,000 - 100 2,000 - 50 2.0 2,000 - 50
Agarose - Low Melt, Separation ≤ 1000 bp, Genetic Performance CertifiedTM [32829]
1,500 - 500 2.0 1,000 - 400 700 - 150 3.0 500 - 100 300 - 70 4.0 150 - 10 50 - 10 5.0 < 30
Agarose - Low Melt, Separation ≥ 1000 bp, Genetic Performance CertifiedTM [32830]
20,000 - 500 0.75 12,000 - 500 16,000 - 300 1.00 8,000 - 300 10,000 - 250 1.25 4,000 - 200 5,000 - 200 1.50 3,000 - 150 2,500 - 100 1.75 2,000 - 100 1,500 - 50 2.00 1,000 - 50
Bioc
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sBiochemicals
1� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): 0.05 - 0.13
Gel Point (1.5%): 36.0 ± 1.5°CRemelt Point (1.5%): 88.0 ± 1.5°CGel Strength (1%): ≥ 1,200 gm/cm2
Gel Strength (1.5%): ≥ 2,500 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.14%
Agarose - Separation > 500 bp, Genetic Performance CertifiedTM
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – Separation ≥ 500 bp, Genetic Performance CertifiedTM (Product No. 75817) 200 400 600 800 1,000 2,000 4,000 6,000 8,000 10,000 12,000 14,000 16,000 18,000 20,000 bp**
TAE Buffer TBE Buffer
0
Preparation of an Agarose Gel (PNs 75817 & 32802):Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer. Example: For an 0.8% gel, add 0.8 gm of agarose and 100 ml of TBE Buffer (1X), to a 200 ml flask. The larger flask insures against the agarose boiling over. Dissolve the agarose in a boiling water bath or in a revolving-plate microwave oven. All the grains of agarose should be dissolved and the solution clear. Cool the solution to 60°C (70°C for concentrations 2% or above) and pour immediately. Allow the gel to set for one-half hour before using. Make sure to use the same electrophoresis buffer in the gel as for the running buffer.
Related Products
TAE Buffer, 10X Solution75904 1L 5L
TBE Buffer, 5X Solution75891 1L 5L
TBE Buffer, 10X Ready-Mixed Powder70454 6x200ml
Genetic Performance Tests: DNase: Passes test RNase: Passes test Restriction Enzyme Digestion: Passes test Ligation: Passes test DNA Binding: Passes test Blotting: Passes test Gel Background: Passes test DNA Resolution: Passes testSeparation Guidelines:Range of separation in 1X TAE: 0.6%: 20,000-1000 bp 0.8%: 12,000-500 bp 1.0%: 8000-300 bp 1.2%: 6000-200 bp 1.5%: 3500-100 bp 2.0%: 2000-50 bp
Range of separation in 1X TBE: 0.6%: 15,000-1000 bp 0.8%: 10,000-500 bp 1.0%: 7000-250 bp 1.2%: 5000-200 bp 1.5%: 3000-100 bp 2.0%: 2000-50 bpApplications:•Separation of DNA or RNA fragments ≥ 500 bp.•PCR analysis.•Recovery of separated DNA or RNA fragments
from gel.•Blotting applications such as Southerns and
Northerns.CAS #9012-36-6
75817 25gm 100gm 250gm 500gm
Biochemicals
Biochemicals
15w w w . u s b w e b . c o m
Agarose - Finger Printing, Genetic Performance CertifiedTM
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – LE (Product No. 32802)
200 400 600 800 1,000 2,000 4,000 6,000 8,000 10,000 12,000 14,000 16,000 18,000 20,000 bp**
TAE Buffer TBE Buffer
0
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free flowing powderEEO (-m
r): ≤ 0.13
Gel Point (1.5%): 36 ± 1.5°CGel Strength (1%): ≥ 1,400 gm/cm2
Moisture: ≤ 7%Residue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.14%
Genetic Performance Tests: DNase: Passes test RNase: Passes test Gel Background: Passes test DNA Resolution: Passes test
Applications:•DNA identity applications such as forensic
testing, tissue typing and paternity determination.
•Analytical separation of DNA or RNA fragments.•Suitable for blotting applications.CAS #9012-36-6
32881 100gm 500gm
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): 0.05 - 0.13
Gel Point (1.5%): 36 ± 1.5°CRemelt Point (1.5%): 88 ± 1.5°CGel Strength (1%): ≥ 1200 gm/cm2
Gel Strength (1.5%): ≥ 2500 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.14%
DNase: Passes testRNase: Passes testGel Background: Passes test
Agarose - LE
Separation Guidelines:Range of separation in 1X TAE: 0.6%: 20,000-1000 bp 0.8%: 12,000-500 bp 1.0%: 8000-300 bp 1.2%: 6000-200 bp 1.5%: 3500-100 bp 2.0%: 2000-50 bpRange of separation in 1X TBE: 0.6%: 15,000-1000 bp 0.8%: 10,000-500 bp 1.0%: 7000-250 bp 1.2%: 5000-200 bp 1.5%: 3000-100 bp 2.0%: 2000-50 bp
Applications:•Routine separation of DNA or RNA fragments.•PCR analysis.•Blotting applications such as Southerns and
Northerns.•Protein analysis such as immunodiffusion,
rocket assays and immuno electrophoresis.CAS #9012-36-6
32802 25gm 100gm 250gm 500gm
Bioc
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ical
sBiochemicals
16 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – Hi-ResTM, Separation ≤ 1000 bp (Product No. 10132) 10 20 30 40 50 60 70 80 90 100 200 300 400 500 600 700 800 900 1,000 1,100 1,200 1,300 1,400 1,500 bp**
TAE Buffer TBE Buffer
0
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free flowing powderEEO (-m
r): ≤ 0.12
Gel Point (3%): ≤ 31°CRemelt Point (3%): ≤ 76°CGel Strength (3%): ≥ 500 gm/cm2
Moisture: ≤ 7%Residue on Ignition: ≤ 0.35%
Agarose - Hi-ResTM, Separation < 500 bp
Sulfate (SO4): ≤ 0.11%
DNase: Passes testRNase: Passes testSeparation Guidelines:Range of separation in 1X TAE: 3.0%: 500-80 bp 4.0%: 300-30 bp 5.0%: 200-10 bpNote: Before use, chill gels at 4°C for 20 hours to
achieve best resolution.
Applications:•Highly resolved separation of DNA or RNA
fragments ≤ 500 bp•PCR analysisCAS #9012-36-6
10133 25gm 100gm 500gm
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.12
Gel Point (3%): ≤ 35.5°CRemelt Point (3%): ≤ 80°CGel Strength (3%): ≥ 1500 gm/cm2
Moisture: ≤ 7%Residue on Ignition: ≤ 0.35%
Agarose - Hi-ResTM, Separation < 1000 bp
Sulfate (SO4): ≤ 0.11%
DNase: Passes testRNase: Passes testSeparation Guidelines:Range of separation in 1X TAE: 1.8%: 1000-500 bp 2.0%: 1500-100 bp 3.0%: 600-150 bp 4.0%: 500-20 bp 4.5%: 350-50 bp 5.0%: 300-10 bp
Range of separation in 1X TBE: 2.0%: 1200-100 bp 3.0%: 700-40 bp 4.0%: 200-20 bp 5.0%: < 100 bpApplications:•Highly resolved separation of DNA or RNA
fragments ≤1000 bp•PCR analysisCAS #9012-36-6
10132 25gm 100gm 500gm
Biochemicals
Biochemicals
17w w w . u s b w e b . c o m
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – Hi-ResTM, Separation ≤ 1500 bp (Product No. 10138)
Related Products Buffers & Stock Biochemicals:
Ethidium Bromide32813 1gm 5gm 25gm
Ethidium Bromide Drops75816 5ml
TAE Buffer, 10X Solution75904 1L 5L
TBE Buffer, 5X Solution75891 1L 5L
TBE Buffer, 10X Ready-Mixed Powder70454 6x200ml
DNA Markers:
PCR Markers, 50 - 2000 bp76710 250µl
DNA Ladder, 100 bp76712 500µl
DNA Ladder, 1 kb Plus76714 500µl
RNA Markers:
RNA Ladder, 0.1 - 1 kb76722 50µl
RNA Marker, 0.2 - 10 kb76724 50µl
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free flowing powderEEO (-m
r): ≤ 0.12
Gel Point (4%): ≤ 40.5°CRemelt Point (4%): ≤ 93°CGel Strength (1.5%): ≥ 2000 gm/cm2
Gel Strength (4%): ≥ 4200 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 50 Np
Agarose - Hi-ResTM, Separation < 1500 bp
Residue on Ignition: ≤ 0.35%Sulfate (SO
4): ≤ 0.11%
DNase: Passes testRNase: Passes testSeparation Guidelines:Range of separation in 1X TAE: 2.0%: 2500 - 700 bp 3.0%: 1200 - 500 bp 4.0%: 700 - 100 bp 5.0%: 250 - 30 bp
Range of separation in 1X TBE: 2.0%: 1500 - 500 bp 3.0%: 800 - 100 bp 4.0%: 500 - 50 bp 5.0%: 250 - 20 bpApplications:•Highly resolved separation of DNA or RNA
fragments ≤ 1500 bp.•PCR analysis.CAS #9012-36-6
10138 25gm 100gm 500gm
Bioc
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sBiochemicals
18 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.12
Gel Point (1.5%): 24 - 28°CRemelt Point (1.5%): ≤ 65.5°CGel Strength (1%): ≥ 250 gm/cm2
Gel Strength (1.5%): ≥ 500 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.12%
Agarose - Low Melt Separation > 1000 bp, Genetic Performance CertifiedTM
Genetic Performance Tests: DNase: Passes test RNase: Passes test In-Gel Restriction Enzyme Digestion: Passes
test In-Gel Ligation: Passes test DNA Binding: Passes test Gel Background: Passes test DNA Resolution: Passes test Agarase Digestion: Passes testSeparation Guidelines:Range of separation in 1X TAE: 0.75%: 20,000 - 500 bp 1.00%: 16,000 - 300 bp 1.25%: 10,000 - 250 bp 1.50%: 5000 - 200 bp 1.75%: 2500 - 100 bp 2.00%: 1500 - 50 bp
Range of separation in 1X TBE: 0.75%: 12,000 - 500 bp 1.00%: 8000 - 300 bp 1.25%: 4000 - 200 bp 1.50%: 3000 - 150 bp 1.75%: 2000 - 100 bp 2.00%: 1000 - 50 bpApplications:•Enzymatic modification of nucleic acids in
re-melted gels.•Separation of DNA or RNA fragments ≥ 1,000 bp.
•In-gel recovery of nucleic acid fragments.•PCR analysis.•Labile sample procedures.•Suitable for cell culture and cloning applications.CAS #9012-36-6
32830 25gm 100gm
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – Low Melt Separation ≥ 1000 bp, Genetic Performance CertifiedTM (Product No. 32830)
Isolating DNA from low-melting temperature agarose (PNs 32829 & 32830):Follow procedure for preparing an agarose gel and be sure to run the electrophoresis at 4°C to keep the gel from melting. Excise the DNA band of interest and transfer it to a microcentrifuge tube. Cut as close to the DNA band as possible to avoid any unnecessary agarose. Add 5 volumes of 20mM Tris-HCl (pH 8.0), 1mM EDTA (pH 8.0) to the tube with the agarose. Cap the tube and heat for 5 minutes at 65°C. The agarose should melt. Cool the solution to room temperature and add an equal volume of equilibrated phenol. Vortex for 20 seconds and spin at 400 x g for 10 minutes (20°C). Remove the aqueous phase (top) to a new microcentrifuge tube. Re-extract with phenol:chloroform and then with chloroform. Precipitate the final aqueous phase with 0.2 volumes of 10mM ammonium acetate and 2 volumes of ethanol. Recover the DNA by centrifugation.
100 200 300 400 500 600 700 800 900 1,000 2,000 4,000 6,000 8,000 10,000 12,000 14,000 16,000 18,000 20,000 bp**0
TAE Buffer TBE Buffer
Related Products
TAE Buffer, 10X Solution75904 1L 5L
TBE Buffer, 5X Solution75891 1L 5L
TBE Buffer, 10X Ready-Mixed Powder70454 6x200ml
Biochemicals
Biochemicals
19w w w . u s b w e b . c o m
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.10
Gel Point (4%): ≤ 35°CRemelt Point (4%): ≤ 65°CGel Strength (4%): ≥ 1000 gm/cm2
Moisture: ≤ 5%Residue on Ignition: ≤ 0.3%Sulfate (SO
4): ≤ 0.12%
Agarose - Low Melt Separation < 1000 bp, Genetic Performance CertifiedTM
Genetic Performance Tests: DNase: Passes test RNase: Passes test In-Gel Restriction Enzyme Digestion: Passes
test In-Gel Ligation: Passes test DNA Binding: Passes test Gel Background: Passes test DNA Resolution: Passes test Agarase Digestion: Passes testSeparation Guidelines:Range of separation in 1X TAE: 2.0%: 1500-100 bp 3.0%: 700-150 bp 4.0%: 300-70 bp 5.0%: 50-10 bp
Range of separation in 1X TBE: 2.0%: 1000-400 bp 3.0%: 500-100 bp 4.0%: 150-10 bp 5.0%: < 30 bpApplications:•Enzymatic modification of nucleic acids in
re-melted gels.•Separation of DNA or RNA fragments ≤ 1,000 bp.
•In-gel recovery of nucleic acid fragments.•PCR analysis.•Labile sample procedures.CAS #9012-36-6
32829 25gm 100gm
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – Low Melt Separation ≤ 1000 bp, Genetic Performance CertifiedTM (Product No. 32829) 10 20 30 40 50 60 70 80 90 100 200 300 400 500 600 700 800 900 1,000 1,100 1,200 1,300 1,400 1,500 bp**
TAE Buffer TBE Buffer
0
Related Products Buffers & Stock Biochemicals:
Ethidium Bromide32813 1gm 5gm 25gm
Ethidium Bromide Drops75816 5ml
TAE Buffer, 10X Solution75904 1L 5L
TBE Buffer, 5X Solution75891 1L 5L
TBE Buffer, 10X Ready-Mixed Powder70454 6x200ml
DNA Markers:
PCR Markers, 50 - 2000 bp76710 250µl
DNA Ladder, 100 bp76712 500µl
DNA Ladder, 1 kb Plus76714 500µl
RNA Markers:
RNA Ladder, 0.1 - 1 kb76722 50µl
RNA Marker, 0.2 - 10 kb76724 50µl
Bioc
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sBiochemicals
�0 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
* Range of separation depends upon the choice of buffer. Migration in TBE buffer is slower, therefore slightly lower concentrations can be used to obtain the same range of separation.
** Size in base pairs
Guide for Separation Ranges*Agarose – Pulse Field (PFGE) (Product No. 32901) 200 400 600 800 1,000 2,000 4,000 6,000 8,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 bp**
TAE Buffer TBE Buffer
0
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.12
Gel Point (1.5%): 36 ± 1.5°CRemelt Point (1.5%): 88 ± 1.5°CGel Strength (1.0%): ≥ 1800 gm/cm2
Gel Strength (1.5%): ≥ 3200 gm/cm2
Moisture: ≤ 10%Turbidity (1.5%): ≤ 40 Np
Agarose - Pulse Field (PFGE)
Residue on Ignition: ≤ 0.25%Sulfate (SO
4): ≤ 0.12%
DNase: Passes testRNase: Passes testSeparation Guidelines:Range of separation in 1X TAE: 0.3%: 40,000 - 3000 bp 0.5%: 22,000 - 2000 bp 0.8%: 15,000 - 1000 bp 1.0%: 10,000 - 500 bp 1.8%: 5000 - 200 bp
Range of separation in 1X TBE: 0.3%: 20,000 - 2000 bp 0.5%: 12,000 - 1500 bp 0.8%: 9000 - 1000 bp 1.0%: 6000 - 500 bp 1.8%: 3000 - 200 bpApplications:•Pulse Field Gel Electrophoresis.•Analytical separation of DNA or RNA fragments.CAS #9012-36-6
32901 25gm 100gm 250gm
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.12
Gel Point (1%): ≤ 20°CRemelt Point (1%): ≤ 63°C
Agarose - Ultra Low Melt
Gel Strength (2%): ≥ 300 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.35%Sulfate (SO
4): ≤ 0.14%
DNase: Passes testRNase: Passes test
Applications:•Labile sample procedures.•Cell culture and cloning applications
(hybridomas).CAS #9012-36-6
32821 10gm 25gm
Biochemicals
Biochemicals
�1w w w . u s b w e b . c o m
Agarose - PFGE Plugs
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderGel Point (1.5%): ≤ 30°CRemelt Point (1.5%): ≤ 70°CMoisture: ≤ 10%DNase: Passes testApplication:•Pulse Field Gel ElectrophoresisCAS #9012-36-6
32903 1gm
Agarose - Fine Resolution
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.05
Gel Point (3%): ≤ 35°CRemelt Point (3%): ≤ 75°CGel Strength (3%): ≥ 300 gm/cm2
Moisture: ≤ 10%Sulfate: ≤ 0.10%DNase: Passes testRNase: Passes testCAS #9012-36-6
73422 25gm 100gm
Agarose - High Gel Strength
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Granular, free flowing powderEEO (-m
r): ≤ 0.12
Gel Point (1.5%): 36 ± 1.5°CRemelt Point (1.5%): 88 ± 1.5°CGel Strength (1%): ≥ 1800 gm/cm2
Gel Strength (1.5%): ≥ 3200 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.25%Sulfate (SO
4): ≤ 0.12%
DNase: Passes testRNase: Passes testApplication:•Analytical separation of DNA or RNA fragmentsCAS #9012-36-6
32880 25gm 100gm 250gm
Agarose - HE
ULTRAPUREPRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): 0.23 - 0.26
Gel Point (1.5%): 36 ± 1.5°CRemelt Point (1.5%): 88 ± 1.5°CGel Strength (1%): ≥ 750 gm/cm2
Gel Strength (1.5%): ≥ 1200 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 1.0%Sulfate (SO
4): ≤ 0.2%
Applications:•Counter current electrophoresis•ImmunodiffusionCAS #9012-36-6
32804 25gm 100gm 250gm 500gm
Agarose - HEEO
ULTRAPUREPRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≥ 0.30
Gel Point (1.5%): 36 ± 1.5°CRemelt Point (1.5%): 88 ± 1.5°CGel Strength (1%): ≥ 700 gm/cm2
Gel Strength (1.5%): ≥ 1100 gm/cm2
Moisture: ≤ 7%Sulfate (SO
4): ≤ 0.2%
Applications:•Counter current electrophoresis•Suitable for electro immunoassays such as
rocket assays•Suitable for immunodiffusionCAS #9012-36-6
32835 25gm 100gm 250gm 500gm
Agarose - HGT
ULTRAPUREPRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): ≤ 0.14
Gel Point (1.5%): 42 ± 1.5°CRemelt Point (1.5%): 87 ± 1.5°CGel Strength (1%): ≥ 900 gm/cm2
Gel Strength (1.5%): ≥ 1200 gm/cm2
Moisture: ≤ 8%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.2%
DNase: Passes testRNase: Passes testApplications:•Cell culture and cloning applications
(hybridomas)•Counter current electrophoresis•Suitable for electro immunoassays such as
rocket assays•Suitable for immunodiffusionCAS #9012-36-6
32805 25gm 100gm 250gm 500gm
Agarose - ME
ULTRAPUREPRODUCT SPECIFICATIONSForm: Granular, free-flowing powderEEO (-m
r): 0.16 - 0.19
Gel Point (1.5%): 36 ± 1.5°CRemelt Point (1.5%): 88 ± 1.5°CGel Strength (1%): ≥ 1000 gm/cm2
Gel Strength (1.5%): ≥ 2200 gm/cm2
Moisture: ≤ 7%Turbidity (1.5%): ≤ 40 NpResidue on Ignition: ≤ 0.5%Sulfate (SO
4): ≤ 0.2%
Applications:•High resolution protein electrophoresis•Immunoelectrophoresis•Electro immunoassays such as rocket assays•Counter current electrophoresisCAS #9012-36-6
32803 25gm 100gm 250gm 500gm
Bioc
hem
ical
sBiochemicals
�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Agarose-CollagenPRODUCT SPECIFICATIONSForm: Soluble collagen packed in gelConcentration: ≥ 3 mg collagen/ml gelStorage: 4°C. Do not freeze.
10682 10ml
Alanine Aminotransferase, Recombinant(r-hALT)
Source: Human recombinant from E. coliPRODUCT SPECIFICATIONSForm: Suspension in 40mM sodium acetate
buffer (pH 5.5), 1mM DTT, 1mM EDTA, 5mM 2-oxoglutarate, 0.1mM pyridoxal-5'-phosphate
Specific Activity: ≥ 500 units/mg proteinPurity: Single band at 55k, as determined by
SDS-PAGE under reducing conditionsFormula Weight: 54,479/subunit as determined
from amino acid sequenceStorage: -20°C
26007 100units
β-AlanineNH
2CH
2CH
2COOH
Formula Weight: 89.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (as is)Identity: By IRChromatography (TLC): HomogeneousLoss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.2%Ammonia (NH
4): ≤ 0.1%
Chloride (Cl): ≤ 0.04%Sulfate (SO
4): ≤ 0.05%
CAS #107-95-9
10665 1kg 5kg
D-AlanineCH
3CH(NH
2)COOH
Formula Weight: 89.09PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : -13.7° to -15.0° (c=10 in 6N HCl)
Loss on Drying: ≤ 0.3%Heavy Metals (Pb): ≤ 0.002%Arsenic (As): ≤ 1 ppmIron (Fe): ≤ 10 ppmCAS #338-69-2
10675 5gm 25gm 100gm
DL-α-AlanineCH
3CH(NH
2)COOH
Formula Weight: 89.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Chromatography (TLC): HomogeneousIdentity: By IRLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.2%Chloride (Cl): ≤ 0.021%Sulfate (SO
4): ≤ 0.03%
Ammonia (NH4): ≤ 0.02%
Iron (Fe): ≤ 30 ppmHeavy Metals: ≤ 10 ppmCAS #302-72-7
10685 1kg 5kg 25kg
L-Alanine, High PurityCH
3CH(NH
2)COOH
Formula Weight: 89.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (dry basis): ≥ 99.0%Identity: By IRChromatography (TLC): HomogeneouspH (2.5%, H
2O): 5.5 - 7.0
[α]25D : +13.7° to +15.1° (c=10, 6N HCl)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.001%Ammonium (NH
4): ≤ 0.02%
Arsenic (As): ≤ 1 ppmChloride (Cl): ≤ 0.02%Iron (Fe): ≤ 0.001%Sulfate (SO
4): ≤ 0.02%
CAS #56-41-7
10703 25gm 100gm 1kg
L-AlanineUSBioAnalyzedCH
3CH(NH
2)COOH
Formula Weight: 89.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (dry basis): 98.5 - 101.5% C
3H
7NO
2
Chromatographic Purity: Passes testIdentity: By IRpH (5%, H
2O, 25°C): 5.5 - 7.0
[α]25D : +13.7° to +15.1° (c=10 in 6N HCl)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.15%Heavy Metals (Pb): ≤ 0.0015%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Sulfate (SO
4): ≤ 0.03%
O.V.I.: Meets requirementsCAS #56-41-7
10705 10gm 25gm 100gm 500gm 1kg
Biochemicals
Biochemicals
�3w w w . u s b w e b . c o m
Albumin, Bovine, Protease-FreeMB GradeProtease FreeSource: Bovine BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay (electrophoresis): ≥ 98.0%Protein: ≥ 96.0%pH (1%, 0.15M NaCl): 6.8 - 7.2Moisture: ≤ 5.0%Residue on Ignition: ≤ 1.5%Fatty Acids (total): ≤ 0.2 mg/gmIgG: Passes testHeavy Metals (Pb): ≤ 10 ppmProtease: Passes testStorage: 4°CCAS #9048-46-8
10867 10gm 50gm 100gm
Albumin, Bovine, Acetylated(Acetylated BSA)
ULTRAPUREMB GradeSource: Bovine BloodPRODUCT SPECIFICATIONSForm: Clear, colorless solution in deionized
water; packed under aseptic, nuclease-free conditions.
Assay: Single band by SDS-polyacrylamide gel electrophoresis
Protein Concentration: 25 - 50 mg/mlContaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testFunctional Test: No inhibition by albumin on
digestion of lambda DNA.Form: Many nucleases are irreversibly inactivated
by acetylation, but the stabilizing action of BSA is unaffected by this procedure.(1,2) Thus, acetylation ensures that the use of albumin does not introduce additional nucleases to reaction mixture.
Solubility: Soluble in waterApplication:•Bovine Serum Albumin is used extensively
by molecular biologists to stabilize labile enzymes such as restriction endonucleases, polymerases and ligases.
References:1. Epstein, C. J. and Goldberger, R. F. (1964)
J. Biol. Chem. 239, 1087-1089.2. Lackey, D. and Linn, S. (1980) Meth. in
Enzymol., 65, 26-28.Storage: -20°CCAS #9048-46-8
10848 25mg 125mg
Albumin, Bovine, Crystallized, pH 5.2Source: Bovine BloodPRODUCT SPECIFICATIONSForm: Crystallized powderAssay (electrophoresis): ≥ 99%Identity: By IRProtein: ≥ 98%pH (7%, 0.15M NaCl): 5.0 - 5.4Moisture (KF) when packaged: ≤ 5.0%Sodium (Na): 0 - 5.0 mg/gmChloride (Cl): 0 - 3.0 mg/gmStorage: -20°CCAS #9048-46-8
10856 1gm 5gm 10gm 100gm
Albumin, Bovine, Cohn Fraction V, pH 5.2(Cold Alcohol Fractionated)
Source: Bovine BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay (electrophoresis): ≥ 96%Protein: ≥ 95%pH (1%, H
2O): 5.0 - 5.4
Residue on Ignition: ≤ 1.5%Loss on Drying: ≤ 6.0%Heavy Metals (Pb): ≤ 50 ppmStorage: 4°C, desiccatedCAS #9048-46-8
10852 50gm 100gm 500gm
Albumin, Bovine, pH 7(Heat-Shock Fractionated)
Source: Bovine BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay (electrophoresis): ≥ 98%Protein: ≥ 96.0%pH (2%, H
2O): 6.5 - 7.5
Loss on Drying: ≤ 5.0%Residue on Ignition: ≤ 2.0%Storage: 4°CCAS #9048-46-8
10857 10gm 50gm 100gm 500gm 1kg
Albumin, Bovine, Fatty Acid Poor, pH 7(Heat-Shock Fractionated)
Source: Bovine BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay (electrophoresis): ≥ 98%Protein: ≥ 96%pH (1%, H
2O): 6.8 - 7.2
Residue on Ignition: ≤ 2.0%Loss on Drying: ≤ 5.0%Free Fatty Acids: ≤ 1.0 mg/gmEsterified Fatty Acids: ≤ 1.0 mg/gmStorage: 4°C, desiccatedCAS #9048-46-8
10858 1gm 5gm 10gm 25gm 100gm
Albumin, Bovine, RIA Grade, pH 7Source: Bovine BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay (electrophoresis): ≥ 98.0%Protein: ≥ 96.0%pH (1%. 0.15M NaCl): 6.8 - 7.2Loss on Drying: ≤ 5.0%Residue on Ignition: ≤ 2.0%IgG: NegativeInsulin: NegligibleFolic Acid: NegligibleT
3, T
4: Negligible
Application:•Suitable for use in radioimmuno assayStorage: 4°CCAS #9048-46-8
10868 5gm 10gm 50gm 100gm
Bioc
hem
ical
sBiochemicals
�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Albumin, Bovine, Low EndotoxinSource: Bovine BloodPRODUCT SPECIFICATIONSForm: Off-white to pale green powderAssay: ≥ 98.0% Albumin (electrophoresis)Protein: ≥ 97% (dry basis)pH (2%, H
2O): 6.5 - 7.5
Endotoxin: ≤ 2.0 Eu/mgHeavy Metals: ≤ 50 ppmLoss on Drying: ≤ 5.0%Residue on Ignition: ≤ 2.0%Solubility (7%, H
2O): Clear, colorless
Application: •Used as a blocking agent or enzyme diluent
for sensitive assays where contaminating proteases would compromise assay results.
Storage: -20°C, protect from moisture.CAS #9048-46-8
10893 10gm 50gm
Albumin, Bovine, 22% SolutionSource: Bovine BloodAseptically filled. Contains 0.1% Sodium Azide as
a preservative.Storage: 4°C
10864 10ml 50ml
Albumin, Bovine, pH 7, Australian Origin
Reagent GradePRODUCT SPECIFICATIONSForm: Lyophilized powderSource: Bovine BloodAssay (electrophoresis): ≥ 98.0%Protein: ≥ 96.0%pH (6%, H
2O): 6.5 - 7.2
Moisture: ≤ 5.0%Residue on Ignition : ≤ 3.0%Fatty Acid: ≤ 0.05%Immunoglobulin: ≤ 0.05%Protease: Passes testStorage: 4°C
10871 5gm 25gm 100gm
Albumin, Bovine, Standard Powder, pH 7, Australian Origin
PRODUCT SPECIFICATIONSForm: Lyophilized powderSource: Bovine BloodAssay (electrophoresis): ≥ 98.0%Protein: ≥ 96.0%pH (6%, H
2O): 6.5 - 7.2
Moisture: ≤ 5.0%Residue on Ignition: ≤ 3.0%Storage: 4°C
10869 5gm 25gm 100gm 500gm
Albumin, Egg(Ovalbumin)
Source: Egg White, Spray DriedPRODUCT SPECIFICATIONSForm: Off-white crystalsProtein (as is): ≥ 80%pH: 6.5 - 8.0Moisture: ≤ 8.0%Salmonella: NegativeCAS #9006-59-1
15460 1lb 5lb 25lb
Albumin, Egg(Ovalbumin)
Source: Chicken EggPRODUCT SPECIFICATIONSForm: White to pale yellow freeze dried powderAssay: ≥ 98.0% (as confirmed by SDS-PAGE)Moisture: ≤ 6.0%Solubility: Soluble in distilled water or dilute
bufferStorage: -20°CCAS #9006-59-1
10865 1gm 5gm
Albumin, Fraction V, Fatty Acid PoorSource: Human BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay (electrophoresis): ≥ 95%Protein: ≥ 96%Moisture (KF): ≤ 5.0%pH (7%, H
2O): 6.8 - 7.2
Sodium (Na): ≤ 15.0 mg/gmChloride (Cl): ≤ 12.0 mg/gmFatty Acids: ≤ 1 mg/gmStorage: 4°C
10881 500mg 1gm
Albumin, Human, Fraction VSource: Human BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderAssay: ≥ 95% albumin (electrophoresis)Protein: ≥ 95%Note: All units of plasma are tested and found
to be negative for antibody to Human T cell Lymphotropic Virus type III (anti-HTLV III) by Elisa and for Hepatitis B Surface Anitgen (HBsAg) by Elisa.
Storage: 4°CCAS #70024-90-7
10878 500mg 1gm 5gm 10gm 100gm
Alcohol Dehydrogenase(E.C.1.1.1.1)Source: YeastPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 300 units/mg proteinUnit Definition: One unit reduces 1 µmol NAD/min
at 25°C, pH 8.8.Contaminating Activities:Carboxylase: ≤ 0.05%GAPDH: ≤ 0.05%PGK: ≤ 0.05%MK: ≤ 0.03%Aldolase: ≤ 0.02%PK: ≤ 0.01%LDH: ≤ 0.01%Storage: -20°C
10895 15,000units 75,000units
Biochemicals
Biochemicals
�5w w w . u s b w e b . c o m
Aldolase(E.C.4.1.2.13)Source: Rabbit MusclePRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionActivity: ≥ 10 units/mg proteinUnit Definition: One unit will convert 1.0 µmol of
Fructose-1,6-Diphosphate to Dihydroxyacetone Phosphate and Glyceraldehyde-3-Phosphate per minute at pH 7.4 and 25°C.
Storage: 4°C
10900 50mg 100mg
Alizarin Red SC
14H
7O
7SNa
Formula Weight: 342.26pH range (acid): 4.0 (yellow) - 6.0 (orange-red)pH range (alkaline): 6.0 (orange-red) - 12.0
(violet)CAS #130-22-3
10910 25gm 100gm
Amido Black 10B(Naphthol Blue Black)
(C.I.20470)C
22H
14N
6O
9S
2Na
2
Formula Weight: 616.49PRODUCT SPECIFICATIONSForm: Dark purple/blue powderIdentity: By IRApplications:•Staining protein in paper chromatography•Histological stain for hemoglobinCAS #1064-48-8
32809 100gm
Aminoacetic AcidSee Glycine, page 235
D-Amino Acid Oxidase(E.C.1.4.3.3)Source: Porcine KidneyForm: Lyophilized powderUnit Definition: One unit oxidizes 1 µmol of
D-Alanine/min at 25°C, pH 8.3.Storage: 4°CCAS #9000-88-8
11030 5mg 25mg
L-Amino Acid Oxidase(E.C.1.4.3.2)Source: Crotalus adamanteus venomForm: Aqueous solutionUnit Definition: One unit oxidizes 1 µmol of
L-Leucine/min at 25°C, pH 7.6.Storage: 4°C
11040 2mg 5mg
DL-α-Amino Adipic AcidStorage: 4°CCAS #542-32-5
11045 250mg 1gm 5gm
4-Amino AntipyreneC
11H
13N
3O
Formula Weight: 203.24PRODUCT SPECIFICATIONSForm: Pale yellow to light amber crystalline
powderAssay: ≥ 98.0%Identity: By IRSolubility (5%, H
2O): Clear solution
Melting Point: 109 ± 2°CCAS #83-07-8
11056 10gm 50gm 100gm
p-Amino Benzoic Acid (PABA)USBioAnalyzedC
7H
7NO
2
Formula Weight: 137.14PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (dry basis): 98.5 - 101.5%Identity (A, B): Passes testsMelting Point: 186 - 189°CLoss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.002%Volatile Diazotizable Substances: ≤ 0.002%Ordinary Impurities: Passes testCAS #150-13-0
11055 200gm 500gm 1kg 5kg
L- -Amino-N-Butyric AcidCH
3CH
2CH(NH
2)COOH
Formula Weight: 103.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (as is)Identity: By IRChromatography (TLC): HomogeneousMoisture (KF): ≤ 1%CAS #1492-24-6
11079 1gm 5gm
2-Aminoethane Sulfonic AcidSee Taurine, page 294
α-Aminoglutaric AcidSee Glutamic Acid, page 231
2-Amino-6-HydroxypurineSee Guanine, page 237
4-Amino-2-HydroxypyrimidineSee Cytosine, page 213
DL-β-Amino-Iso-Butyric AcidH
2NCH
2CH(CH
3)COOH
Formula Weight: 103.12CAS #10569-72-9
11160 1gm 25gm
∆-Aminolevulinic Acid, HydrochlorideC
5H
9NO
3•HCl
Formula Weight: 167.59Storage: -20°CCAS #5451-09-2
11170 100mg 500mg 1gm
Bioc
hem
ical
sBiochemicals
�6 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
2-Amino-2-Methyl-1-Propanol(AMP)
CH3C(CH
3)(NH
2)CH
2OH
Formula Weight: 89.14PRODUCT SPECIFICATIONSForm: Clear, viscous liquidAssay: ≥ 95.0% (as is)Identity: By IRSolubility (10%, H
2O): Passes test
CAS #124-68-5
11186 100gm 500gm
2-Aminopurine, Free BaseC
5H
5N
5
Formula Weight: 135.13CAS #452-06-2
11235 100mg 250mg 1gm
6-Amino PurineSee Adenine, page 173
Aminosuccinic AcidSee Aspartic Acid, page 194
3-Amino-1,2,4-TriazoleC
2H
4N
4
Formula Weight: 84.08CAS #61-82-5
11245 25gm 100gm
Ammonium Acetate
ULTRAPUREACS Reagent GradeCH
3COONH
4
Formula Weight: 77.08PRODUCT SPECIFICATIONSForm: White/clear crystalsAssay: ≥ 97.0%pH (5%, H
2O): 6.7 - 7.3
Insoluble Matter: ≤ 0.005%Residue on Ignition: ≤ 0.01%Chloride (Cl): ≤ 5 ppmNitrate (NO
3): ≤ 0.001%
Sulfate (SO4): ≤ 0.001%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmSolubility (5M, H
2O): Clear, colorless
Applications:•Used in the precipitation of DNA•Said to help decrease the co-precipitation of
dNTPs•Asymmetric PCR precipitationStock Solution, 7.5M: Ammonium Acetate: 289 gm dH
2O:175 ml
Adjust volume with water to 500 ml. Filter sterilize.CAS #631-61-8
11251 500gm 1kg
Ammonium Acetate (NH4OAc), 5M Solution
ULTRAPUREMB GradeForm: 5M solution of ammonium acetate
(NH4OAc) in high purity dH
2O. Solution is
0.2 µm filtered and dispensed into durable, square Nalgene bottles.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75901 100ml 500ml
Ammonium ChlorideACS Reagent GradeNH
4Cl
Formula Weight: 53.49PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.5%pH (5%, H
2O, 25°C): 4.5 - 5.5
Insoluble Matter: ≤ 0.005%Residue on Ignition: ≤ 0.01%Calcium (Ca): ≤ 0.001%Magnesium (Mg): ≤ 5 ppmHeavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 2 ppmPhosphate (PO
4): ≤ 2 ppm
Sulfate (SO4): ≤ 0.002%
11248 500gm 2.5kg
Ammonium Citrate, Dibasic, Anhydrous
ACS Reagent Grade(NH
4)2HC
6H
5O
7
Formula Weight: 226.19PRODUCT SPECIFICATIONSForm: CrystallineAssay: 98.0 - 103.0%Insoluble Matter: ≤ 0.005% Residue on Ignition: ≤ 0.01% Chloride (Cl): ≤ 0.001%Heavy Metals (Pb): ≤ 5 ppm Iron (Fe): ≤ 0.001% Oxalate (C
2O
4): Passes test
Phosphate (PO4): ≤ 5 ppm
Sulfur Compounds (SO4): ≤ 0.005%
11234
Ammonium Iron (III) Citrate, BrownSee Ferric Ammonium Citrate, Brown, page 226
Ammonium Iron (III) Citrate, GreenSee Ferric Ammonium Citrate, Green, page 226
Biochemicals
Biochemicals
�7w w w . u s b w e b . c o m
Ammonium Molybdate, TetrahydrateACS Reagent Grade(NH
4)6Mo
7O
24•4H
2O
Formula Weight: 1235.86PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 81.0 - 83.0%Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.002%Nitrate (NO
3): Passes test
Arsenate, Phosphate, and Silicate (SiO2):
≤ 0.001%Phosphate (PO
4): ≤ 5 ppm
Sulfate (SO4): ≤ 0.02%
Heavy Metals (Pb): ≤ 0.001%Magnesium (Mg): ≤ 0.005%Potassium (K): ≤ 0.002%Sodium (Na): ≤ 0.01%CAS #12027-67-7
11252 500gm 2.5kg
Ammonium Persulfate (APS)(Ammonium Peroxydisulfate)
ULTRAPUREACS Reagent Grade(NH
4)2S
2O
8
Formula Weight: 228.19PRODUCT SPECIFICATIONSForm: Off-white crystalline powderAssay: ≥ 98.0%Residue on Ignition: ≤ 0.05%Insoluble Matter: ≤ 0.005%Titratable Free Acid: ≤ 0.04 meq/gmChloride and Chlorate (Cl): ≤ 0.001%Heavy Metals (Pb): ≤ 0.005%Iron (Fe): ≤ 0.001%Manganese (Mn): ≤ 0.5 ppmApplication:•A high speed initiator used to catalyze
acrylamide polymerizationWorking Stock Solution (10%): Ammonium Persulfate: 1 gm dH
2O: to 10 ml
This solution should be stored at 4°C and prepared fresh weekly.
CAS #7727-54-0
76322 10gm 100gm 1kg
Ammonium Phosphate, DibasicACS Reagent Grade(NH
4)2HPO
4
Formula Weight: 132.06PRODUCT SPECIFICATIONSForm: CrystallineAssay: ≥ 98.0%pH (5%, H
2O, 25°C): 7.7 - 8.1
Insoluble Matter: ≤ 0.005%Ammonium Hydroxide Precipitate: ≤ 0.005%Chloride (Cl): ≤ 0.001%Nitrate (NO
3): ≤ 0.003%
Sulfate (SO4): ≤ 0.01%
Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%Potassium (K): ≤ 0.005%Sodium (Na): ≤ 0.005%
11247 500gm 2.5kg
Ammonium Phosphate, MonobasicACS Reagent GradeNH
4H
2PO
4
Formula Weight: 115.03PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%pH (5%, H
2O, 25°C): 3.8 - 4.4
Insoluble Matter: ≤ 0.005%Ammonium Hydroxide Precipitate: ≤ 0.005%Chloride (Cl): ≤ 5 ppmNitrate (NO
3): ≤ 0.001%
Sulfate (SO4): ≤ 0.01%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 0.001%Potassium (K): ≤ 0.005%Sodium (Na): ≤ 0.005%
11249 500gm 2.5kg
Ammonium Sulfate
ULTRAPUREACS Reagent Grade(NH
4)2SO
4
Formula Weight: 132.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 5.0 - 6.0
Insoluble Matter: ≤ 0.005%Residue on Ignition: ≤ 0.005%Chloride (Cl): ≤ 5 ppmNitrate (NO
3): ≤ 0.001%
Phosphate (PO4): ≤ 5 ppm
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmApplication:•Used in the purification of enzymes and other
proteins by precipitationCAS #7783-20-2
11254 1kg 5kg
Amoxicillin, TrihydrateC
16H
19N
3O
5S•3H
2O
Formula Weight: 419.45PRODUCT SPECIFICATIONSForm: White to off-white powderActivity: 900 - 1050 µg of C
16H
19N
3O
5S/mg
(dry basis)Identity: By IRCrystallinity: Meets requirementspH (2 mg/ml): 3.5 - 6.0 (c=2 in H
2O)
Water: 11.5% to 14.5%Dimethylaniline: Meets requirementsCAS #61336-70-7
11256 10gm 25gm
AMPSee Adenosine-5'-Monophosphate, Disodium
Salt, page 174
Bioc
hem
ical
sBiochemicals
�8 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Ampicillin, Trihydrate
ULTRAPUREUSBioAnalyzedC
16H
19N
3O
4S•3H
2O
Formula Weight: 403.45 (trihydrate)PRODUCT SPECIFICATIONSForm: White crystalline powderActivity: 900 - 1050 µg of C
16H
19N
3O
4S/mg
(dry basis)Activity: 765 - 924 µg of C
16H
19N
3O
4S/mg (as is)
Identity: By IRpH (10 mg/ml, H
2O): 3.5 - 6.0
Water: 12-15% (trihydrate)Dimethylaniline: Meets requirementCrystallinity: Meets requirementsMode of Action: Binds to and inhibits many of the
enzymes in the bacterial membrane that are involved in cell wall synthesis.
Stock Solution: 50 mg/ml in H
2O
Titrate slowly with 0.5M NaOH until dissolved. Working Concentration: 20 - 60 µg/mlCAS #7177-48-2
11257 5gm 25gm 100gm 1kg
Ampicillin, Sodium Salt
ULTRAPUREUSBioAnalyzedC
16H
18N
3NaO
4S
Formula Weight: 371.39PRODUCT SPECIFICATIONSForm: White to off-white powderActivity: 845 µg - 988 µg of C
16H
19N
3O
4S/mg
(dry basis)Activity: 828 µg - 988 µg of C
16H
19N
3O
4S/mg
(as is)Identity (A, B): Passes testspH (10 mg/ml): 8.0 - 10.0Moisture (KF): ≤ 2.0%Dimethylaniline: Meets requirementsMethylene chloride: ≤ 0.2%Storage: Controlled room temperature, in tight
containers.CAS #69-52-3
11259 5gm 25gm 100gm
-Amylase(E.C.3.2.1.1)Source: Bacillus subtilisPRODUCT SPECIFICATIONSForm: Light tan to brown colored powderStorage: 4°C
10990 25gm 50gm 100gm 500gm
α-Amylase(E.C.3.2.1.1)Source: Porcine PancreasForm: Suspension in 0.5M saturated sodium
chloride (pH 7.0) with 3mM calcium chloride.PRODUCT SPECIFICATIONSActivity: ≥ 1800 units/mg proteinUnit Definition: One unit produces 1 µmol of
reducing groups from soluble starch/min at 25°C, pH 6.9.
Storage: 4°C
10995 25mg 50mg 100mg
AmylopectinSource: CornPRODUCT SPECIFICATIONSForm: White powderIdentity: By IRpH (10% dispersion in cold H
2O): 4.5 - 6.5
Loss on Drying: ≤ 12%CAS #9037-22-3
11270 1kg
Antibiotic G-418 SulfateSee G-418 Sulfate, page 228
Antimycin AStorage: 4°CCAS #1397-94-0
11375 10mg 25mg 50mg 100mg
Aprotinin
ULTRAPURESource: Bovine LungC
284H
432N
84O
79S
7
Formula Weight: 6512PRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 6000 Kallikrein Inhibitor units/mg
proteinPurity (electrophoresis): Single bandLoss on Drying: ≤ 6.0%Applications:•A useful protease inhibitor against kallikrein,
trypsin, plasmin and chymotrypsin•Not effective against papainWorking Concentration: 1-2 mg/ml Soluble in 0.01M HEPES (pH 8.0)Note: Avoid repeated freezing and thawing of
stock solutions.Storage: 4°C in air-tight containers.CAS #9087-70-1
11388 10mg 25mg 100mg
D-(–)-ArabinoseC
5H
10O
5
Formula Weight: 150.13CAS #10323-20-3
11395 25gm 100gm
DL-ArabinoseC
5H
10O
5
Formula Weight: 150.13CAS #20235-19-2
11400 10gm
Biochemicals
Biochemicals
�9w w w . u s b w e b . c o m
L- (+)-ArabinoseC
5H
10O
5
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (GC): ≥ 96%Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.5%Chloride (Cl): ≤ 100 ppmSulfate (SO
4): ≤ 100 ppm
Arsenic (As): ≤ 3 ppmIron (Fe): ≤ 50 ppmHeavy Metals (Cu): ≤ 5 ppmCAS #5328-37-0
11405 25gm 100gm 1kg
L-ArabinoseHigh Purity GradeC
5H
10O
5
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: White crystalline powder[α]20
D : +103.0° ± 1.5° (c=4 in H
2O, NH
3)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Arsenic (As): ≤ 0.5 ppmIron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #5328-37-0
11406 25gm 100gm 1kg
ArbutinC
12H
16O
7
Formula Weight: 272.25PRODUCT SPECIFICATIONSForm: White powderIdentity: By IR[α]25
D : -63° to -66° (c=3 in H
2O)
Moisture (KF): ≤ 5.0%CAS #497-76-7
11450 5gm 10gm 25gm 100gm
D-Arginine, HydrochlorideC
6H
14N
4O
2•HCl
Formula Weight: 210.66PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: 99.0% - 101.0%Residue on Ignition: ≤ 0.1%CAS #627-75-8
11475 1gm 5gm 10gm
DL-Arginine, HydrochlorideNH
2C(NH)NH(CH
2)3CH(NH
2)COOH•HCl
Formula Weight: 210.66PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98%Chromatography (TLC): HomogeneousIdentity: By IRLoss on Drying: ≤ 8.0%Storage: 4°CCAS #7200-25-1
11480 5gm 25gm 100gm
L-ArginineC
6H
14N
4O
2
Formula Weight: 174.20PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IR[α]20
D : +26.9° to +27.9° (c=8 in 6N HCl)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.3%Heavy Metals (Pb): ≤ 15 ppmAmmonium (NH
4): ≤ 0.02%
Arsenic (As2O
3): ≤ 1 ppm
Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 30 ppmSulfate (SO
4): ≤ 0.03%
CAS #74-79-3
11490 100gm 500gm 1kg
L-Arginine, HydrochlorideNH
2C(NH)NH(CH
2)3CH(NH
2)COOH•HCl
Formula Weight: 210.66PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5%Chromatography (TLC): HomogeneousIdentity: By IR[α]20
D : +21.4° to +23.6° (c=8 in 6N HCl)
Loss on Drying: ≤ 0.20%Residue on Ignition: ≤ 0.10%Chloride (Cl): 16.5 to 17.1%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 20 ppmO.V.I.: Meets requirementsCAS #1119-34-2
11500 100gm 500gm 1kg
L-Ascorbic Acid(Vitamin C)
USBioAnalyzedC
6H
8O
6
Formula Weight: 176.12PRODUCT SPECIFICATIONSForm: White to slightly yellow crystalline powderAssay: 99.0 - 100.5%Identity A: By KBrIdentity B: Passes test[α]25
D : +20.5° to +21.5° (c=10 in H
2O)
Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.002%O.V.I.: Meets requirementsCAS #50-81-7
11545 500gm 1kg 5kg
Ascorbyl PalmitateC
22H
38O
7
Formula Weight: 414.53PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderAssay: ≥ 95.0% (dry basis)[α]25
D : +21° to +24° (c=1 in Ethanol)
Melting Point: 109 - 117°CLoss on Drying: ≤ 2.0%Residue on Ignition: ≤ 0.1%Arsenic (As): ≤ 3 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #137-66-6
11570 1kg
Bioc
hem
ical
sBiochemicals
30 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
D-AsparagineNH
2COCH
2CH(NH
2)COOH•H
2O
Formula Weight: 150.13
11580
DL-Asparagine, MonohydrateNH
2COCH
2CH(NH
2)COOH•H
2O
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRChromatography (TLC): HomogeneousLoss on Drying: ≤ 12%CAS #3130-87-8
11585 100gm 1kg 5kg
L-Asparagine, AnhydrousNH
2COCH
2CH(NH
2)COOH
Formula Weight: 132.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Chromatography (TLC): HomogeneousIdentity: By IR[α]20
D : +33° to +36° (c=2 in 10% HCl)
Loss on Drying: ≤ 1.0%CAS #70-47-3
11590 100gm 500gm 1kg
L-Asparagine, MonohydrateC
4H
8N
2O
3•H
2O
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (as is)Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +33° to +36° (c=10 in 3N HCl)
Loss on Drying: ≤ 12.5%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmArsenic (As): ≤ 3 ppmIron (Fe): ≤ 20 ppmSulfate (SO
4): ≤ 0.025%
Lead (Pb): ≤ 5 ppmCAS #5794-13-8
11595 100gm 1kg 5kg
D-Aspartic Acid(D-Amino-Succinic Acid)
HOOCCH2CH(NH
2)COOH
Formula Weight: 133.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : -25.9° to -27.0° (c=10 in 2N HCl)
Loss on Drying: ≤ 0.25%CAS #1783-96-6
11600 25gm 100gm
DL-Aspartic Acid(DL-Amino-Succinic Acid)
HOOCCH2CH(NH
2)COOH
Formula Weight: 133.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): HomogeneousLoss on Drying: ≤ 0.3%CAS #617-45-8
11605 1kg
L-Aspartic AcidC
4H
7NO
4
Formula Weight: 133.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +24.5° to +26.0° (c=8 in 6N HCl)
Loss on Drying: ≤ 0.25%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmArsenic (As): ≤ 3 ppmCAS #56-84-8
11620 100gm 1kg 5kg
L-Aspartic AcidCP GradeNH
2CH(COOH)CH
2COOH
Formula Weight: 133.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IR[α]20
D : +24.5° to +25.5° (c=8 in 6N HCl at 20°C)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Sulfate (SO
4): ≤ 0.02%
Heavy Metals (Pb): ≤ 10 ppmArsenic (As): ≤ 1 ppmChloride (Cl): ≤ 0.02%Iron (Fe): ≤ 10 ppmAmmonium (NH
4): ≤ 0.02%
CAS #56-84-8
11625 100gm 1kg
L-Aspartic Acid, Monopotassium Salt(Potassium Aspartate)
C4H
6NO
4K
Formula Weight: 171.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRpH (10%, H
2O): 5.5 - 7.5
[α]20D : +17.1° to +20.5° (as is) (c=8 in 6N HCl)
Loss on Drying (110°C): ≤ 10.0%Heavy Metals (Pb): ≤ 10 ppmArsenic (As
2O
3): ≤ 1 ppm
Storage: Protect from moisture in air-tight containers; material is very hygroscopic.
CAS #1115-63-5
11630 100gm 1kg
Biochemicals
Biochemicals
31w w w . u s b w e b . c o m
L-Aspartic Acid, Sodium Salt, Monohydrate(Sodium Aspartate)
NH2CH(COOH)CH
2COONa•H
2O
Formula Weight: 173.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +18° to +21° (c=8 in 6N HCl, dry basis)
Loss on Drying: ≤ 0.5%Heavy Metals (Pb): ≤ 10 ppmArsenic (As): ≤ 1 ppmChloride (Cl): ≤ 0.05%Iron (Fe): ≤ 30 ppmSulfate (SO
4): ≤ 0.05%
CAS #3792-50-5
11635 100gm 1kg
L-Aspartic Acid, Magnesium Salt, Dihydrate
(C4H
6NO
4)2Mg•2H
2O
Formula Weight: 324.53 (dihydrate)PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 95.0% (dry basis)[α]20
D : +22° to +25° (c=8 in 6N HCl, dry basis)
Moisture (KF): ≤ 17%Heavy Metals (Pb): ≤ 10 ppmArsenic (As
2O
3): ≤ 1 ppm
Chloride (Cl): ≤ 0.021%Sulfate (SO
4): ≤ 0.050%
Iron (Fe): ≤ 30 ppmCAS #2068-80-6
11631 25gm 100gm 1kg
ATPSee Adenosine-5'-Triphosphate, page 174
AuxinSee 2,4-Dichlorophenoxyacetic Acid, page 220 Indole-3-Acetic Acid, page 242 Indole-3-Butyric Acid, page 242 Indole-3-Butyric Acid Potassium Salt, page 242 α-Naphthalene Acetic Acid, page 260 β-Naphthoxyacetic Acid, page 260
AvidinAntagonist - BiotinSource: Egg WhiteUnit Definition: One unit will bind 1 µg of Biotin
at pH 8.9.Storage: 4°C
11680 1mg 5mg 10mg 100mg
Avidin-Streptavidin(Streptavidin)
Source: Streptomyces avidiniPRODUCT SPECIFICATIONSForm: White, lyophilized powderActivity: 11 to 16 units/mgPurity: ≥ 95%Unit Definition: One unit is that amount of protein
required to bind one microgram of D-biotin.Applications:•A tetrameric protein containing four high affinity
binding sites for biotin•Used with biotin in the purification of DNA-
binding proteinsStorage: -20°C, protect from moisture (powder);
4°C (reconstituted)
11681 5mg 10mg
Avidin-Alkaline Phosphatase Conjugate
See Streptavidin-Alkaline Phosphatase Conjugate, page 291
Azo Violet(4-(4-Nitrophenylazo)resorcinol)
C12
H9N
3O
4
Formula Weight: 259.22 PRODUCT SPECIFICATIONSForm: Red, orange or red-orange powderIdentity: By IRSolubility: Passes testVisual Transition Interval: pH 11.0 (yellow) to
pH 13.0 (violet)
19576 25gm 100gm
r-B2MSee B2 Microglobin, Recombinant, page 259
BacitracinUSBioAnalyzedC
66H
103N
17O
16S
Formula Weight: 1422.69PRODUCT SPECIFICATIONSForm: White to pale buff powderActivity: ≥ 40 units of bacitracin/mg (as is)Identity: Passes testpH (10,000 units/ml): 5.5 - 7.5Loss on Drying: ≤ 5.0%CAS #1405-87-4
11805 1gm 5gm 25gm
Bacitracin-ZincUSBioAnalyzedPRODUCT SPECIFICATIONSForm: Tan crystalline powderActivity: ≥ 40 Bacitracin units/mg (as is)Identity: Passes testpH (100 mg/ml [saturated], H
2O): 6.0 - 7.5
Loss on Drying: ≤ 5.0%Zinc Content: 2.0 to 10.0% (dry basis)CAS #1405-89-6
11810 1gm 5gm
Bacterial Culture MediaLB Agar, Ready-Made Powder, page 246LB Broth, Ready-Made Powder, page 247Luria Agar, Ready-Made Powder, page 249Luria Broth, Ready-Made Powder, page 250NZCYM Broth, Ready-Made Powder, page 266Terrific Broth, Ready-Made Powder, page 295YPD Agar, Ready-Made Powder, page 311YPD Broth, Ready-Made Powder, page 3112X YT Agar, page 3112X YT Broth, page 311
Bioc
hem
ical
sBiochemicals
3� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Bacteriological Peptone(Peptones)
ULTRAPUREPRODUCT SPECIFICATIONSForm: PowderMoisture: ≤ 5.0%Ash: 4.0 - 6.65%pH: 6.0 - 7.5Amino Nitrogen: 5.4 - 6.6%Total Nitrogen: 12.4 - 13.8%Protein (N x 6.38): 79.5 - 88.0%An extremely high grade of peptone prepared
specifically for general bacteriological purposes.
CAS #73049-73-7
20048 1lb 5lb 25lb
Bacteriological Peptone II
20042 250gm 1kg 5kg
Basic Fuchsin(Rosaniline Chloride)
(C.I.42510)C
20H
20N
3Cl
Formula Weight: 337.85CAS #632-99-5
32811 10gm 25gm
BCIPSee 5-Bromo-4-Chloro-3-Indolyl Phosphate,
page 200
Benedict’s ReagentPRODUCT SPECIFICATIONSKit Consists of: PN 11885 Premix Benedict’s Reagent Powder PN 13839 Cupric SulfateReagent Solution Preparation:1. Dissolve 273 gm Benedict’s Reagent Powder in
distilled water with heat & q.s. to 850 ml.2. Dissolve 17.4 gm Cupric Sulfate in 100 ml hot
distilled water.3. Mix both solutions with rapid stirring and heat
and q.s. to 1 liter.
11885 1pk(for500tests)
Benzoic AcidC
6H
5COOH
Formula Weight: 122.12PRODUCT SPECIFICATIONSForm: White to off-white crystalline powder or
flakesAssay: ≥ 98.5%Identity: By IRMelting Point: 124 ± 2°CMoisture: ≤ 2.0%CAS #65-85-0
11905 500gm 1kg
Benzoic Acid, Sodium Salt(Sodium Benzoate)
C6H
5COONa
Formula Weight: 144.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0% - 100.5% (dry basis)Identification: Passes testAlkalinity: Passes testMoisture (KF): < 1.5%Heavy Metals: ≤ 0.001%O.V.I.: Meets requirementsCAS #532-32-1
11910 500gm 1kg
Benzoyl-DL-Arginine Naphthylamide Hydrochloride
C23
H25
N5O
2•HCl
Formula Weight: 439.94Storage: -20°C
11940 500mg 1gm 5gm 10gm
-N-Benzoyl-DL-Arginine-p-Nitroanilide, Hydrochloride(BAPNA)
C19
H22
N6O
4•HCl
Formula Weight: 434.88PRODUCT SPECIFICATIONSForm: Pale cream powderChromatography (TLC): HomogeneousIdentity: By IRMoisture (KF): ≤ 2.0%Storage: 4°CCAS #911-77-3
11945 500mg 1gm 5gm 10gm
N6-Benzyl Adenine(6-Benzylaminopurine)
C12
H11
N5
Formula Weight: 225.25Inhibitor of respiratory kinases in plants. Aids
in the extension of post harvest life in green vegetables.
CAS #1214-39-7
12015 1gm 5gm 25gm
BES(N,N-bis[Hydroxyethyl]-2-Aminoethanesulfonic Acid)
(HOCH2CH
2)2NCH
2CH
2SO
3H
Formula Weight: 213.25PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 7.15 ± 0.15A (250 nm, 10%, 1 cm): ≤ 0.1Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #10191-18-1
12051 25gm 50gm 100gm 1kg
Biochemicals
Biochemicals
33w w w . u s b w e b . c o m
Bestatin[(2S,3R)-3-Amino-3-Hydroxy-4- Phenylbutanoyl]-L-Leu
C16
H24
N2O
4
Formula Weight: 308.37Source: MicrobialPRODUCT SPECIFICATIONSForm: White crystalline powderChromatography (TLC): HomogeneousApplications:•A specific inhibitor of aminopeptidases and
other exopeptidases•Can be used in wheat germ and reticulocyte-
lysate in vitro translation systemsStorage: -20°C
12052 5mg 10mg 25mg 100mg
Betaine, AnhydrousC
5H
11NO
2
Formula Weight: 117.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRMoisture (KF): ≤ 5.0%Heavy Metals (Pb): ≤ 10 ppmChloride (Cl): ≤ 50 ppmNote: Material is hygroscopic.CAS #107-43-7
12080 100gm 1kg
Betaine, HydrochlorideCH
3CH
2CH
2(NH
2)CHCOOH•HCl
Formula Weight: 153.61PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals: ≤ 10 ppmArsenic (As): ≤ 2 ppmCAS #590-46-5
12085 100gm 1kg
Betaine, 5M Solution
ULTRAPUREMB GradeForm: 5M solution of betaine in deionized dH
2O.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testFunctional Test: Functionally tested for use in
PCR.Storage: -20°C
77507 1.5ml 5x1.5ml 10ml
BHA(Butylated Hydroxyanisole; 2,6-Ditert-butyl-4-Hydroxyanisole)
C11
H16
O2
Formula Weight: 180.24CAS #25013-16-5
12090 500gm
BHT(Butylated Hydroxytoluene; 2,6-Ditert-butyl-p-cresol)
C15
H24
OFormula Weight: 220.35CAS #128-37-0
10295 100gm 500gm
Bicine(N,N-bis[2-Hydroxyethyl]glycine)
(CH2CH
2OH)
2NCH
2COOH
Formula Weight: 163.17PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IRpKa (20°C): 8.35 ± 0.15A (250 nm, 10%, 1 cm): ≤ 0.1Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #150-25-4
12091 50gm 100gm 1kg
Bile Bacteriological(Bile Salts)
12105 1lb
BilirubinC
33H
36N
4O
6
Formula Weight: 584.66PRODUCT SPECIFICATIONSForm: Bright orange fine powderAssay: ≥ 97.5% (By UV, E
max = 61.2 x 103, as is)
Identity: By IRMoisture (KF): ≤ 2.0%λ
max: 453 ± 2 nm
Storage: Store protected from light.CAS #635-65-4
12110 250mg 500mg 1gm 5gm
D-Biotin(Vitamin H)
ULTRAPUREC
10H
16N
2O
3S
Formula Weight: 244.31PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 97.5% (as is)Identity (A, B): Passes tests[α]25
D : +89° to +93° (c=2 in 0.1N NaOH)
Melting Point: 229 - 232°C (decompose)Heavy Metals (Pb): ≤ 10 ppmStorage: Store in air-tight containers.CAS #58-85-5
12115 500mg 1gm 25gm
Bis-AcrylamideSee N, N'-Methylene-bis-Acrylamide, page 255
Bis-(2-Mercaptoethyl)-Sulfone(BMS)
ULTRAPUREReagent GradeC
4H
10O
2S
3
Formula Weight: 186.32PRODUCT SPECIFICATIONSForm: Off-white crystalline powderPurity (ex S): ≥ 99% (dry basis)Elemental Analysis: C, H, SStorage: -20°C
77157 250mg 1gm
Bioc
hem
ical
sBiochemicals
3� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Bis-Tris(bis[2-Hydroxyethyl]imino-tris[hydroxymethyl] methane)
ULTRAPURE(HOCH
2CH
2)2NC(CH
2OH)
3
Formula Weight: 209.24PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 6.50 ± 0.15A (280 nm, 1M, 1 cm): ≤ 0.4Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #6976-37-0
12112 100gm 500gm 1kg
Bis-Tris Propane(1,3-bis-[Tris (Hydroxymethyl) Methylamino]-Propane)
ULTRAPURE(HOCH
2)3CNH(CH
2)3NHC(CH
2OH)
3
Formula Weight: 282.33PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRSpectral Ratio (280 nm, 0.1M, 1 cm): ≤ 0.05Melting Point: 164 - 170°CMoisture (KF): ≤ 1.0%
12113 25gm 100gm
Bleomycin SulfatePRODUCT SPECIFICATIONSForm: White powderAssay: 1.4 - 2.1 Bleomycin units/mgStorage: 4°C
12149 15units
Blocking Buffer, 5X Solution
ULTRAPUREForm: 5X specially formulated solution containing
SDS, sodium caseinate, Tris, NaCl and 0.02% sodium azide in deionized water.
pH (25°C): 9.5 ± 0.5Contaminant Testing: Tested for fungal and
bacterial contaminationStorage: Store buffer at room temperature for up
to 3 months or at 4°C for up to 12 months. To dissolve any precipitated SDS, warm the buffer at 37°C for 60 min with gentle agitation. A small amount of insoluble precipitate is normal and should not affect results. (Do not heat in a microwave.)
Application: •For use in isotopic or non-isotopic detection of
DNA or RNA to deliver high signal, low noise results on nylon membrane blots
75354 600ml
Blotting ReagentsBlocking Buffer, 5X Solution, page 198Denhardt’s Solution, 50X, page 214SSC, 20X Solution, page 290SSPE, 20X Solution, page 290TBS, 20X Solution, pH 7.4, page 294TBS with Tween (TBST), 20X Solution, pH 7.4,
page 295
Boric AcidACS Reagent GradeH
3BO
3
Formula Weight: 61.83PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.5%Insoluble in MeOH: ≤ 0.005%Nonvolatile with MeOH: ≤ 0.05%Chloride (Cl): ≤ 0.001%Phosphate (PO
4): ≤ 0.001%
Sulfate (SO4): ≤ 0.01%
Calcium (Ca): ≤ 0.005%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%CAS #10043-35-3
12329 500gm 1kg
Boric Acid
ULTRAPUREMB GradeH
3BO
3
Formula Weight: 61.83PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.5%Insoluble in MeOH: ≤ 0.005%Nonvolatile with MeOH: ≤ 0.05%Sulfate (SO
4): ≤ 0.01%
Heavy Metals (Pb): ≤ 0.001%Arsenic (As): ≤ 1 ppmCalcium (Ca): ≤ 0.005%Chloride (Cl): ≤ 0.001%Iron (Fe): ≤ 0.001%Phosphate (PO
4): ≤ 0.001%
Functional Test: Meets or exceeds criteria for high quality sequencing gel electrophoresis with Sequenase T7 DNA Polymerase.
DNase (endo): Passes testDNase (exo): Passes testRNase: Passes testProtease: Passes testCAS #10043-35-3
76324 500gm 1kg
Boric Acid, Sodium Salt(Sodium Borate, Decahydrate; Sodium Tetraborate, Decahydrate)
ACS Reagent GradeNa
2B
4O
7•10H
2O
Formula Weight: 381.37PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.5 - 105.0%pH (0.01M, H
2O, 25°C): 9.15 - 9.20
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.001%Phosphate (PO
4): ≤ 0.001%
Sulfate (SO4): ≤ 0.005%
Calcium (Ca): ≤ 0.005%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 5 ppmCAS #1303-96-4
21614 500gm 1kg 5kg
Br-dUSee 5-Bromo-2'-Deoxyuridine, page 201
Biochemicals
Biochemicals
35w w w . u s b w e b . c o m
Brefeldin A
25103
Brewers YeastSee Yeast-Brewers, page 310
Brij® 35(Polyoxyethylene Lauryl Ether)
ULTRAPUREPRODUCT SPECIFICATIONSForm: White waxy solidAcid number: ≤ 5.0Hydroxyl number: 40 - 60Moisture: ≤ 3.0%Flash Point: ≥ 300°FPour Point: Approx. 33°CSpecific Gravity (25°C): Approx. 1.05Detergent for Stein-Moore chromatography.CAS #9002-92-0
12335 100gm 500gm 1kg 5kg
Brilliant Blue G(Coomassie® Brilliant Blue G-250)
ULTRAPURE(C.I.42655)C
47H
48N
3S
2O
7Na
Formula Weight: 854.02PRODUCT SPECIFICATIONSForm: Dark blue/purple crystalline powderDye content: Approx. 60%Loss on Drying: ≤ 8%λ
max: 607 nm ± 1 nm
εmax
: 38 x 103
Application:•Used for quantitative staining of proteins on
polyacrylamide electrophoresis gelsCAS #6104-58-1
32812 25gm 100gm
Brilliant Blue R(Coomassie® Brilliant Blue R-250)
ULTRAPURE(C.I.42660)C
45H
44N
3O
7S
2Na
Formula Weight: 825.97PRODUCT SPECIFICATIONSForm: Dark purple/violet powderIdentity: By IRλ
max: 586 ± 2 nm
εmax
: ≥ 45.0 x 103
Moisture (KF): ≤ 6.0%Application:•Used for quantitative staining of proteins on
polyacrylamide electrophoresis gelsCAS #6104-59-2
32826 25gm 100gm
5-Bromo-3-Indolyl-β-D Galactopyranoside(Indigal)
ULTRAPUREC
14H
16BrNO
6
Formula Weight: 374.28PRODUCT SPECIFICATIONSForm: White powderIdentity: By IRMelting Point: 218 ± 2°CSolubility: Soluble in DMFStorage: -20°C, protected from lightCAS #97753-82-7
17542 100mg 1gm
5-Bromo-4-Chloro-3-Indolyl- -D-Galactopyranoside(X-α-GAL)
ULTRAPUREC
14H
15BrClNO
6
Formula Weight: 408.63PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (TLC): ≥ 99%Chromatography (TLC): Homogeneous Identity: By IRSolubility (1%, MeOH): Clear and colorless[α]25
D : +145.0 ± 3.0° (c=0.5, DMF:H
2O 1:1)
Moisture (KF): ≤ 1.0%Storage: -20°C, protect from moistureCAS #107021-38-5
26200 100mg 500mg
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside(X-GAL)
ULTRAPUREC
14H
15BrClNO
6
Formula Weight: 408.63PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (HPLC): ≥ 99%Chromatography (TLC): HomogeneousIdentity: By IRSolubility (2%, DMF): Clear and colorlessMelting Point: 232 ± 2°CApplications:•A histochemical substrate for β-Galactosidase•A substrate for blue/white colony screening.
Effective concentration is approximately 50 µg/ml.
Stock Concentration: 20 mg/ml in dimethylformamide in a glass or polypropylene tube.
Storage: -20°C, protect from moisture.CAS #7240-90-6
10077 100mg 250mg 1gm 2gm 5gm
Bioc
hem
ical
sBiochemicals
36 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
5-Bromo-4-Chloro-3-Indolyl- -D-Glucuronic Acid, Cyclohexylammonium Salt(X-GLUC)
ULTRAPUREC
20H
26BrClN
2O
7
Formula Weight: 521.79PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IRMoisture (KF): ≤ 5%Applications:•Substrate for β-glucuronidase histochemical
analysis•Used for the chromogenic determination of β-glucuronidase (GUS) gene fusion marker
•Soluble in water: DMF in 1:1 ratioTypical Substrate Solution: 100mM phosphate buffer (pH 7.0) 10mM EDTA (pH 7.0) 0.5mM potassium ferrocyanide (pH 7.0) 0.5mM potassium ferricyanide (pH 7.0) 1-2mM 5-bromo-4-chloro-3-indolyl-β-D-
glucuronideReferences:1. Conchie, J., Findlay, J. and Levvy, G. A. (1959)
Biochem. J. 71, 318-325.2. Fishman, W. H. (1955) Adv in Enz. 16, 361-
409.3. Jefferson, R. A., Kavanagh, T. A. and Bevan,
M. W. (1987) EMBO J. 6, 3901-3907.4. Jefferson, R. A., Burgess, S. M. and Hirsh, D.
(1986) Proc. Natl. Acad. Sci. USA 83, 8447-8451.
5. Pearson, B., Andrews, M. and Grose, F. (1961) Proc. Soc. Exp. Biol. Med. 108, 619-623.
6. Windholz, M. ed (1976) Merck Index, (Merck, Sharp & Dohme, Rahway, N.J.), 654.
7. Lillie, R. D. ed (1977) Biological Stains (Williams & Wilkins Co., Baltimore), 452.
8. Lojda, Z. (1970) Histochemie 23, 266-288.9. Meyerowitz, E. M., personal communication.Storage: Store at -20°C; protect from light.CAS #114162-64-0
12388 10mg 25mg 100mg
5-Bromo-4-Chloro-3-Indolyl Phosphate, Disodium Salt(BCIP)
ULTRAPUREC
8H
4BrClNNa
2O
4P
Formula Weight: 370.43PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderIdentity: By IRChromatography (TLC): HomogeneousStorage: -20°C, protect from moisture.CAS #102185-33-1
12387 25mg 50mg 100mg 500mg 1gm
5-Bromo-4-Chloro-3-Indolyl Phosphate, p-Toluidine Salt
ULTRAPUREC
8H
6BrClNO
4P•C
7H
9N
Formula Weight: 433.62PRODUCT SPECIFICATIONSForm: White to off-white powderIdentity: By IRSolubility (5 mg/ml DMF): Passes testMelting Point: 196 ± 2°CStorage: 4°CCAS #6578-06-9
12390 100mg 500mg 1gm
Bromocresol Green, Sodium Salt
ULTRAPUREACS Reagent GradeC
21H
13Br
4O
5SNa
Formula Weight: 720.00PRODUCT SPECIFICATIONSForm: Brown to orange powderClarity of Solution (0.1%, H
2O): Passes test
Visual Transition Interval: pH 3.8 - Yellow pH 5.4 - BlueApplication: •Used as a tracking dye in alkaline agarose gel
electrophoresisLoading Buffer (6X): 6mM EDTA 300mM NaOH 18% Ficoll (in water) 0.15% Bromocresol Green 0.25% Xylene CyanolCAS #62625-32-5
12355 5gm 10gm 25gm
Bromocresol Purple, Free AcidC
21H
16Br
2O
5S
Formula Weight: 540.20PRODUCT SPECIFICATIONSForm: Light salmon/beige powderInsoluble Matter: Passes testVisual Transition Interval: pH 5.2 - Yellow pH 6.8 - PurpleCAS #115-40-2
12361 10gm 25gm 100gm
Bromocresol Purple, Sodium SaltC
21H
15Br
2O
5SNa
Formula Weight: 562.20PRODUCT SPECIFICATIONSForm: Red-orange powderInsoluble Matter: Passes testVisual Transition Interval: pH 5.2 - Yellow pH 6.8 - PurpleCAS #62625-30-3
12360 10gm 25gm 100gm
Biochemicals
Biochemicals
37w w w . u s b w e b . c o m
5-Bromo-2'-Deoxyuridine(Br-dU)
C9H
11BrN
2O
5
Formula Weight: 307.09PRODUCT SPECIFICATIONS Form: White to off-white powderAssay (UV): ≥ 97%Identity: By IRλ
max: 280 nm ± 1 nm
εmax
: 9.250 x 103 ± 3%Spectral Ratios: A
250/A
260: 0.50 ± 0.05
A280
/A260
: 1.90 ± 0.05Moisture (KF): ≤ 1.0%Storage: -20°CCAS #59-14-3
12400 500mg 1gm
Bromophenol Blue, Sodium Salt
ULTRAPUREACS Reagent GradeC
19H
9Br
4NaO
5S
Formula Weight: 691.94PRODUCT SPECIFICATIONSForm: PowderClarity of Solution: Passes testVisual Transition Interval: pH 3.0 - Yellow pH 4.6 - BlueApplications:•Used as a tracking dye in agarose and
acrylamide gel electrophoresis•Used in the stop solution in DNA sequencing kitsCAS #62625-28-9
12370 5gm 10gm
Bromothymol Blue, Free AcidACS Reagent GradeC
27H
28Br
2O
5S
Formula Weight: 624.38PRODUCT SPECIFICATIONSForm: Pale purple crystalline powderClarity of Solution: Passes testVisual Transition Interval: pH 6.0 - Yellow pH 7.6 - BlueCAS #76-59-5
12374 5gm 10gm 25gm
Bromothymol Blue, Sodium SaltACS Reagent GradeC
27H
27Br
2NaO
5S
Formula Weight: 646.36PRODUCT SPECIFICATIONSForm: Dark greenish gold crystalline powderClarity of Solution: Passes testVisual Transition Interval: pH 6.0 - Yellow pH 7.6 - BlueCAS #34722-90-2
12375 5gm 10gm 25gm
BSASee Albumin, Bovine, page 187
BuffersBlocking Buffer, 5X Solution, page 198Glycerol Tolerant Gel (GTG) Buffer, 20X Solution,
page 234Glycerol Tolerant Gel (GTG) Buffer, 20X Ready-
Mixed Powder, page 234HEPES, 1M Solution, pH 7.3, page 239PBS, 10X Solution, pH 7.4, page 268RapidRun™ Agarose Buffer, 20X Solution, page
282TAE Buffer, 10X Solution, page 293TAE Buffer, 50X Solution, page 293TBE Buffer, 5X Solution, page 294TBE Buffer, 10X Ready-Mixed Powder, page 294TE Buffer, 1X Solution, page 295TE Buffer, 50X Solution, page 295Tris, 2M Solution, page 302Tris, 2M Solution, pH 7.5, page 302Tris-Glycine (TG) Buffer, 10X Solution, page 301Tris-Glycine-SDS (TGS) Buffer,10X Solution, page
301Tris/HCl, 1M Solution, pH 7.0, page 302Tris/HCl, 1M Solution, pH 7.5, page 302Tris/HCl, 1M Solution, pH 8.0, page 302
t-Butyl CarbazateC
5H
12N
2O
2
Formula Weight: 132.16Storage: 4°CCAS #870-46-2
12462
Cacodylic Acid, Sodium Salt(Dimethylarsenic Acid, Sodium Salt; Sodium Cacodylic)
(CH3)2AsO
2Na•3H
2O
Formula Weight: 214.03 (trihydrate)PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.5% (dry basis)Solubility (10%, H
2O): Clear and colorless
Chloride (Cl): ≤ 0.1%Sulfate (SO
4): ≤ 0.3%
Application: •An integral component of terminal
deoxynucleotidyl transferase bufferNote: Material is hygroscopicCAS #124-65-2
12488 25gm 100gm 500gm
Caffeine, Anhydrous(1,3,7-Trimethylxanthine)
C8H
10N
4O
2
Formula Weight: 194.19PRODUCT SPECIFICATIONSForm: White powderAssay: 98.5 - 101.0%Identity (A, B): Passes testsMelting Point: 235 - 239°CLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals: ≤ 10 ppmReadily Carbonizable Substances: Passes testOther Alkaloids: Passes testChromatographic Purity: Passes testO.V.I.: Passes testCAS #58-08-2
12520 100gm 250gm 1kg
Calcium Chloride, AnhydrousACS Reagent GradeCaCl
2
Formula Weight: 110.98PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 96.0%Titratable Base: ≤ 0.006 meq/gCAS #10043-52-4
12354 500gm 2.5kg
Bioc
hem
ical
sBiochemicals
38 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Calcium Chloride, DihydrateACS Reagent GradeCaCl
2•2H
2O
Formula Weight: 147.01PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 105.0%pH (5%, H
2O, 25°C): 4.5 - 8.5
Insoluble Matter: ≤ 0.01%Oxidizing Substances (NO
3): ≤ 0.003%
Sulfate (SO4): ≤ 0.01%
Ammonium (NH4): ≤ 0.005%
Barium (Ba): ≤ 0.005%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 0.001%Magnesium (Mg): ≤ 0.005%Potassium (K): ≤ 0.01%Sodium (Na): ≤ 0.02%Strontium (Sr): ≤ 0.1%
12531 500gm 2.5kg
Calcium Chloride, DihydrateUSBioAnalyzedCaCl
2•2H
2O
Formula Weight: 147.01PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 107.0% (as is)Identity: Passes testspH (5%, H
2O): 4.5 - 9.2
Aluminum: ≤ 1 ppmIron, Aluminum, and Phosphate: Passes testHeavy Metals: ≤ 0.001%Limit of Magnesium and Alkali Salts: ≤ 1.0%O.V.I.: Passes test
12532
D-Calcium Pantothenate(Pantothenic Acid, Calcium Salt; Vitamin B
5)
USBioAnalyzed(C
9H
16NO
5)2Ca
Formula Weight: 476.53PRODUCT SPECIFICATIONSForm: White powderIdentity (A, B): Passes tests[α]25
D : +25.0° to +27.5° (c=5 in H
2O, dry basis)
Loss on Drying: ≤ 5.0%Heavy Metals (Pb): ≤ 20 ppmOrdinary Impurities: Passes testAlkalinity: Passes testNitrogen Content (N): 5.7 to 6.0%Calcium Content (Ca): 8.2 to 8.6%Residual Solvents: Passes testCAS #137-08-6
12550 100gm 500gm 1kg
DL-Calcium Pantothenate(Pantothenic Acid, Calcium Salt; Vitamin B
5)
C18
H32
CaN2O
10
Formula Weight: 476.53CAS #6381-63-1
12555 100gm 1kg
Canavalia Ensiformis Agglutinin-Peroxidase Conjugate(Concanavalin)
Source: JackbeanPRODUCT SPECIFICATIONSForm: Yellow/amber liquidSpecificity: α-D-Mannopyranoside, α-D-GlucopyranosideProtein: 2 mg/mlNote: Stored in 0.05M Tris with 0.004M CaCl
2,
0.15M NaCl, pH 7.0 - 7.2. Mitogenic.Storage: -20°CCaution: Do not inhale lectins or allow skin
contact.
34246 1mg 5mg
CantharidinC
10H
12O
4
Formula Weight: 196.20CAS #56-25-7
12600 25mg 100mg 500mg
Carbenicillin, Disodium SaltC
17H
16N
2Na
2O
6S
Formula Weight: 422.36PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 92.0% (dry basis)Identity: Passes testpH (10 mg/ml): 6.0 - 7.5Moisture (KF): ≤ 5.0%Storage: 4°CCAS #4800-94-6
12678 250mg 1gm 5gm
CAPS3-(Cyclohexylamino)-1-Propanesulfonic Acid
ULTRAPURE(C
6H
11)NH(CH
2)3SO
3H
Formula Weight: 221.32PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRA (250 nm, 0.5M, 1 cm): ≤ 0.25Loss on Drying: ≤ 10%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #1135-40-6
12656 25gm 100gm 1kg
Carbonic Anhydrase(E.C.4.2.1.1)Source: Bovine ErythrocytesFormula Weight: 30.00PRODUCT SPECIFICATIONSForm: Lyophilized powder, salt-freeActivity: ≥ 3,000 units/mg (dry weight)Unit Definition: One unit=2x (T
0-T)/T (Method of
Wilbur and Anderson, [1948] J. Biol. Chem. 176, 147.)
Storage: 4°C, protect from moisture.
12710 50mg 100mg 250mg
Carboxymethyl Cellulose, Sodium SaltThis is a water soluble polymer prepared from
cellulose.CAS #9004-32-4
12730 1lb 5lb 25lb
Carboxypeptidase Y(E.C.3.4.16.5)Source: YeastForm: A useful enzyme in the sequence analysis
of proteins and peptides.Unit Definition: One unit hydrolyzes 1 µmol of
CBZ-Phe-Leu/min at 25°C, pH 6.5.Solubility: Soluble in water and phosphate bufferNote: Please inquire for bulk pricing.Storage: -20°C
12758 1mg 5mg 10mg
Biochemicals
Biochemicals
39w w w . u s b w e b . c o m
Carboxyphenyl PhosphateHOOCC
6H
4OPO
3H
2
Formula Weight: 218.10CAS #6064-83-1
12760 1gm 5gm
DL-Carnitine HCl(Vitamin B
7)
C7H
15NO
3•HCl
Formula Weight: 197.66PRODUCT SPECIFICATIONSForm: White/clear crystalline powder Assay: ≥ 98.5%Identity: By IRLoss on Drying: ≤ 1%CAS #461-05-2
12795 25gm 100gm 1kg
α-CaseinPrepared by urea fractionation. For the assay of
Fibrinolysin.Storage: -20°CCAS #9000-71-9
12820 250mg 1gm
Casein, Hammarsten
ULTRAPUREFormula Weight: 75,000 - 100,000PRODUCT SPECIFICATIONSForm: Cream to light yellow, lyophilized powderProtein: ≥ 95%Loss on Drying: ≤ 10.0%CAS #9000-71-9
12840 100gm 1lb 5lb 25lb
Casein (High Nitrogen)Prepared from New Zealand casein exclusivelyPRODUCT SPECIFICATIONSForm: White to off-white powderProtein (dry basis): ≥ 95.0%CAS #9000-71-9
12845 1lb 5lb 25lb 100lb
Casein Hydrolysate, Enzymatic Digest
ULTRAPUREPRODUCT SPECIFICATIONSForm: PowderMoisture: ≤ 6%pH: 6.6 - 7.5Amino Nitrogen: 3.7 - 4.8%Total Nitrogen: 11.5 - 14.2%Protein (N x 6.38): 73.3 - 90.6%Ash: ≤ 6.5%
12855 1lb 5lb 25lb
Casein Hydrolysate, Acid Digest(Peptones)
Form: White to off-white powderDescription: Acid digest of casein
12850 1lb 5lb 25lb
Casein Hydrolysate, Acid Digest - Low Ash
Low Ash - BacteriologicalPRODUCT SPECIFICATIONSProtein (N x 6.38): ≥ 73%pH: 5.5 - 7.0Moisture: ≤ 6.0%Amino Nitrogen: ≥ 4.0%Total Nitrogen: ≥ 11.5%Ash: ≤ 10%Microbiological Analysis Standard Plate Count <10,000 cfu/gm Coliform: <10 cfu/gm Salmonella*: None detected in 25 gm Yeast & Mold: ≤ 100 cfu/gm * Specification is guaranteed, but not tested
on each lot.CAS #65072-00-6
12852 1lb 5lb 25lb
Casein, Sodium(Sodium Caseinate)
PRODUCT SPECIFICATIONSForm: White to off-white powderProtein (dry basis): ≥ 93%Ash: ≤ 4.2%Moisture: ≤ 5.0%pH (5%, H
2O): 6.4 - 7.0
CAS #9005-46-3
12865 1lb 5lb 25lb 100lb
Casein - Vitafree™PRODUCT SPECIFICATIONSForm: Cream colored powderProtein (N x 6.25): ≥ 95.0%Loss on Drying: ≤ 12.0%Specifically prepared as a source of high quality
protein in diets needed to produce vitamin deficiencies.
12866 5lb 25lb
Catalase(E.C.1.11.1.6)Source: Bovine LiverPRODUCT SPECIFICATIONSForm: Lyophilized powder, approx. one-third
sucroseIdentity: By IRActivity: ≥ 3000 units/mg proteinProtein Concentration: Approx. 50%Unit Definition: One unit decomposes 1 µmol of
H2O
2/min at 25°C, pH 7.0.
Storage: 4°C
12885 500mg 1gm 5gm 10gm
Catalase(E.C.1.11.1.6)Source: FungalPRODUCT SPECIFICATIONSForm: Clear, brown liquidActivity: ≥ 1000 units/mlUnit Definition: One unit is the amount of enzyme
which will decompose 264 mg of hydrogen peroxide in one hour at 25°C, pH 7.0.
Storage: 4°C
12880 50ml
Bioc
hem
ical
sBiochemicals
�0 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
D-(+)-CellobioseC
12H
22O
11
Formula Weight: 342.30PRODUCT SPECIFICATIONSForm: White to off-white powderAssay (HPLC): ≥ 98 area-%Identity: By IR[α]20
D : +34.0° ± 1.0° (c = 1 in H
2O, 15 hours)
Loss on Drying: ≤ 2.0% Heavy Metals (Pb): ≤ 10 ppmCAS #528-50-7
13235 5gm 10gm 25gm 100gm 500gm
Cellulase(E.C.3.2.1.4)Source: Aspergillus nigerUnit Definition: One unit produces a change
in the relative fluidity in a defined sodium carboxymethyl cellulose substrate of 1 in 5 min at 40°C, pH 4.5.
Storage: 4°C
13285 25gm 100gm
Cellulose, Non-Nutritive Fiber For Lab Animal Diets
See Celufil, page 204
Celufil-HydrolyzedThis material has no caloric value.
13293 5lb 25lb 50lb
Celufil-Non Nutritive BulkA fine mesh cellulose filler and binder with
minimal nutritive value for laboratory diets.
13292 5lb 25lb 50lb
CephalexinC
16H
17N
3O
4S•H
2O
Formula Weight: 365.40PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 95.0 - 101.0% (dry basis)Identity: By IRMoisture (KF): ≤ 10%Storage: Ambient, in tight containersCAS #23325-78-2
13294 10gm
Cesium Chloride
ULTRAPUREMB GradeCsClFormula Weight: 168.36PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: 99.999%Refractive Index (50% w/w, 20°C): 1.389 - 1.391A (260 nm, 50% w/v, 1 cm): ≤ 0.02Insoluble Matter: NegligibleSilicon (Si): ≤ 1 ppmSulfate (SO
4): ≤ 5 ppm
Barium (Ba): ≤ 2 ppmCalcium (Ca): ≤ 3 ppmLithium (Li): ≤ 1 ppmMagnesium (Mg): ≤ 1 ppmPotassium (K): ≤ 3 ppmRubidium (Rb): ≤ 4 ppmSodium (Na): ≤ 3 ppmStrontium (Sr): ≤ 1 ppmAluminum (Al): ≤ 1 ppmChromium (Cr): ≤ 1 ppmIron (Fe): ≤ 5 ppmLead (Pb): ≤ 2 ppmManganese (Mn): ≤ 1 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testApplication:•Used for density gradient high speed
centrifugation separation of λ phage, M13 and plasmid DNA
Densities in CsCl: Double-stranded DNA: density (P) = (0.098)
[G + C] + 1.660 gm/cm3 mole Sample Density RFI (Supercoiled) ds DNA 1.709 gm/cm3
RFII (Nicked) ds DNA 1.54 gm/cm3
ss DNA 1.726 gm/cm3
ss RNA 1.90 gm/cm3
Protein 1.33 gm/cm3
Note:1. Nicked DNA binds more ethidium bromide than
supercoiled DNA.2. Ethidium bromide at saturation decreases
density of ds DNA ~ 0.15 gm/cm3.3. More ethidium bromide bound = greater
reduction in density.Typical Cesium Chloride Solutions: Step gradients prepared in SM* or H
2O; for
(100 ml) Density CsCl SM or Refractive (P) (gm) H
2O (ml) Index
1.45 60 85 1.377 1.50 67 82 1.382 1.70 75 75 1.399*SM—phage dilution buffer (100mM NaCl, 8mM
MgSO4•7H
2O, 50mM Tris-HCl, pH 7.5, 0.01%
gelatin).Cesium Chloride RNA Isolation Method:A. Guanidine Thiocyanate Homogenization Buffer
for RNA Isolation: 4.0M Guanidine Thiocyanate, 0.1M Tris, pH 7.5, 1.0% 2-mercaptoethanol
B. Cesium Chloride RNA Isolation Solution: Most researchers layer their RNA homogenates onto a cushion of 5.7M Cesium Chloride.
C. RNA Resuspension Buffer: TE, pH 7.5.Reference:1. Schildkraut, C. L., Marmur, J. and Doty, P.
(1962) J. Mol. Biol. 4, 430-443.CAS #7647-17-8
75822 5gm 25gm 100gm 500gm 1kg
Cesium Hydroxide, MonohydrateCsOH•H
2O
Formula Weight: 167.93 (Monohydrate)CAS #35103-79-8
13330 25gm 100gm
Cetyl Alcohol(1-Hexadecanol; Palmityl Alcohol)
CH3(CH
2)15
OHFormula Weight: 242.44CAS #36653-82-4
13350 100gm 500gm
Biochemicals
Biochemicals
�1w w w . u s b w e b . c o m
Cetyl Trimethyl Ammonium Bromide(CTAB)
[CH3(CH
2)15
N(CH3)3]Br
Formula Weight: 364.46PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% as isIdentity: By IRpH (10%, H
2O): 3.0 - 7.0
Moisture (KF): ≤ 1.0%Melting Point: 232 - 237°CApplications:•Cationic detergent, soluble in H
2O and readily
soluble in alcohol•Used in the preparation and purification of
genomic DNA from bacteria•Complexes with both polysaccharide and
residual protein•Also used for DNA mini preps for sequencingCAS #57-09-0
13353 100gm 500gm 1kg
CHAPS(3-[ (3-Cholamidopropyl)-Dimethylammonio]-1-Propane Sulfonate)
ULTRAPUREC
32H
58N
2O
7S
Formula Weight: 614.88PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (N analysis): ≥ 98%Identity: By IRChromatography (TLC): HomogeneousCritical Micelle Concentration (CMC): 4 to 8mMStorage: 4°C, protected from lightCAS #75621-03-3
13361 1gm 10gm
CHAPSO(3-[ (3-Cholamidopropyl)-Dimethylammonio]-2-Hydroxy-1-Propane Sulfonate)
C32
H58
N2O
8S
Formula Weight: 630.88PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (C analysis): ≥ 96%Identity: By IRChromatography (TLC): HomogeneousMoisture (KF): ≤ 4%Critical Micelle Concentration (CMC): 4 to 8mMStorage: 4°C, protected from lightCAS #82473-24-3
13369 1gm
Charcoal-Activated Norit® AA decolorizing carbon.CAS #7440-44-0
13365 5lb 10lb
Charcoal - Activated(NORIT® SX 4)
An acid washed carbon.PRODUCT SPECIFICATIONSMoisture (as packed): ≤ 10%Molasses Decolorizing No.: ≤ 570Iron (Fe): ≤ 200 ppmCAS #7440-44-0
13370 5lb 10lb
CHES(2-[N-Cyclohexylamino]ethanesulfonic Acid)
(C6H
11)NH (CH
2)2SO
3H
Formula Weight: 207.29PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 9.50 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.3Loss on Drying: ≤ 1.0%Chloride (Cl): ≤ 0.005%Sulfate (SO
4): ≤ 0.005%
Iron (Fe): ≤ 5 ppmLead (Pb): ≤ 10 ppmCAS #103-47-9
13371 100gm 1kg
Chicken Intestine Alkaline Phosphastase
E.C.3.1.3.1Source: Chicken IntestinePRODUCT SPECIFICATIONSForm: Lyophilized powder salt freeUnit Definition: One unit hydrolyzes one µmole of
o-carboxyphenyl phosphate/minute at 25°C, pH 8.8.
Storage:-20°C, desiccated
10925 1gm 5gm
Chitin(Poly-N-Acetyl Glucosamine)
PRODUCT SPECIFICATIONSForm: Unbleached coarse flakesLoss on Drying: ≤ 10%CAS #1398-61-4
13385 100gm 500gm 1kg
ChitosanSource: Crab ShellsCAS #9012-76-4
13392 10gm 25gm 100gm
Chloramphenicol
ULTRAPUREUSBioAnalyzedC
11H
12Cl
2N
2O
5
Formula Weight: 323.13PRODUCT SPECIFICATIONSForm: White to grey-white crystalline powderAssay: 97.0 - 103.0% (as is)Identity (A, B): Passes testsChromatographic Purity: Passes testMelting Point: 149 - 153°CpH (2.5%, H
2O): 4.5 - 7.5
Crystallinity: Passes testApplications:•Used in large-scale plasmid DNA preparation of
low to moderate copy number plasmids. Also used in high copy plasmids to limit the bulk and viscosity of the cell lysates
•Used in chloramphenicol acetyl transferase (CAT) assay
Mode of Action:•Binds to the ribosomal 50S subunit and inhibits
protein synthesis.•Soluble in methanol, ethanol.Stock Solution: 34 mg/ml in ethanol for plasmid
DNAsWorking Concentration: 25-170 µg/ml;
100 mg/ml in ethanol for CAT assaysCAS #56-75-7
23660 5gm 25gm 100gm 1kg
Bioc
hem
ical
sBiochemicals
�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Chlorophenol RedC
19H
12O
5SCl
2
Formula Weight: 423.27PRODUCT SPECIFICATIONSForm: Brown powderλ
max (pH 3.8): 434 ± 1 nm
εmax
(pH 3.8): ≥ 20,000Loss on Drying: ≤ 6%Visual Transition Interval: pH 5.2 - Yellow pH 6.8 - RedCAS #4430-20-0
13550 1gm 5gm 10gm
Chlorotetracycline, HydrochlorideUSBioAnalyzedC
22H
23ClN
2O
8•HCl
Formula Weight: 515.34PRODUCT SPECIFICATIONSForm: Yellow crystalline powderActivity: ≥ 900 µg of C
22H
23ClN
2O
8•HCl/mg (as is)
Identity (A, B): Passes testspH (10 mg/ml, H
2O): 2.3 - 3.3
[α]25D : -235° to -250° (c=0.5 in H
2O)
Loss on Drying: ≤ 2.0%Storage: Ambient in tight light-resistant
containersCAS #64-72-2
13535 5gm 25gm 100gm 250gm
3-[(3-Cholamidopropyl)-Dimethylammonio]-2-Hydroxy-1-Propane Sulfonate
See CHAPSO, page 205
Cholecalciferol (Vitamin D
3, Activated 7-Dehydrocholesterol)
USBioAnalyzedC
27H
44O
Formula Weight: 384.64PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 97.0 - 103.0%Identity (A, B, C, D): Passes tests[α]25
D : +105° to +112° (c=0.8 in EtOH)
O.V.I.: Meets the requirementsStorage: 4°C, under Nitrogen, protect from light.CAS #67-97-0
13394 250mg 1gm 5gm 25gm
CholesterolC
27H
46O
Formula Weight: 386.65PRODUCT SPECIFICATIONSForm: White to faintly yellow crystalline powderIdentity: Passes testSolubility in alcohol (1%): Passes test[α]20
D : -34° to -38° (c=2 in dioxane)
Acidity: Passes testMelting Range: 147 - 150°CLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%CAS #57-88-5
13610 25gm 100gm 1kg
CholesterolReagent GradeC
27H
46O
Formula Weight: 386.65Storage: 4°CCAS #57-88-5
13580 5gm 25gm 100gm
Cholesterol CaprylateC
35H
60O
2
Formula Weight: 512.85PRODUCT SPECIFICATIONSForm: White to off white crystalline powderIdentity: By IRCAS #1182-42-9
13570 10gm 25gm
Cholic Acid, Sodium Salt(Sodium Cholate)
C24
H39
NaO5
Formula Weight: 430.55PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 97.0% (dry basis)Identity: By IRLoss on Drying: ≤ 5.0%CAS #361-09-1
13630 10gm 25gm 100gm 500gm 1kg
Choline BitartrateC
9H
19NO
7
Formula Weight: 253.25PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRMoisture (KF): ≤ 0.5%Heavy Metals (Pb): ≤ 10 ppmCAS #87-67-2
13405 100gm 500gm
Choline ChlorideC
5H
14ClNO
Formula Weight: 139.62PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRMoisture (KF): ≤ 0.5%Residue on Ignition: ≤ 0.05%Heavy Metals (Pb): ≤ 10 ppm1,4-Dioxane: Passes testProtect from moisture. This material is
hygroscopic.CAS #67-48-1
13410 200gm 500gm 1kg 5lb 25lb
Biochemicals
Biochemicals
�3w w w . u s b w e b . c o m
Choline Dihydrogen CitrateC
11H
21NO
8
Formula Weight: 295.29PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRMoisture (KF): ≤ 0.3%Residue on Ignition: ≤ 0.05%Heavy Metals (Pb): ≤ 10 ppmStore in air-tight containers. Material is
hygroscopic.CAS #77-91-8
13415 500gm 1kg 5kg
Chondroitin Sulfate, Sodium Salt(Chondroitin-6-Sulfate)
Source: Shark CartilagePRODUCT SPECIFICATIONSForm: White to off-white powderLoss on Drying: ≤ 10%pH: 5.5 - 7.5Residue on Ignition: 23.0 - 31.0%Nitrogen (N): 2.5 - 3.8%Sulfur (S): 5.5 - 7.5%Arsenic (As): ≤ 2 ppmHeavy Metals: ≤ 40 ppmCAS #9082-07-9
13645 5gm 25gm 100gm
α-Chymotrypsin(E.C.3.4.21.1)Source: Bovine PancreasForm: Lyophilized powder, soluble at 1 mg/ml in
0.001N HClDescription: Free of low molecular weight
contaminants, prepared by the procedure of Yapel, et al. (1966) J. Am. Chem. Soc., 88, 2573.
Unit Definition: One unit hydrolyzes 1 µmol BTEE/min at 25°C, pH 7.8.
Storage: 4°CCAS #9004-07-3
11010 100mg
-Chymotrypsin(E.C.3.4.21.1)Source: Bovine PancreasPRODUCT SPECIFICATIONSForm: Lyophilized powderChymotrypsin Activity: ≥ 1000 units/mg
(dry basis)Storage: -20°CCAS #9004-07-3
13680 1gm 5gm 10gm
Chymotrypsinogen ASource: Bovine PancreasUnit Definition: One unit hydrolyzes 1 µmol BTEE/
min at 25°C, pH 7.8.Storage: 4°C
13695 100mg 1gm 5gm
Citric Acid, AnhydrousUSBioAnalyzedC
6H
8O
7
Formula Weight: 192.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.5 - 100.5% (dry basis)Clarity of Solution: Passes testColor of Solution: Passes testIdentity: By IRMoisture (KF): ≤ 1.0%Residue on Ignition: ≤ 0.1%Readily Carbonizable Substances: Passes testHeavy Metals (Pb): ≤ 0.001%Oxalate: Passes testSulfate (SO
4): Passes test
CAS #77-92-9
13729 500gm 1kg
Citric Acid, Trisodium Salt, Dihydrate(Citric Acid, Sodium Salt; Sodium Citrate, Dihydrate)
ACS Reagent GradeNa
3C
6H
5O
7•2H
2O
Formula Weight: 294.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 7.0 - 9.0
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.003%Sulfate (SO
4): ≤ 0.005%
Ammonia (NH3): ≤ 0.003%
Calcium (Ca): ≤ 0.005%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppm
21604 500gm 2.5kg
Citric Acid, Trisodium Salt, Dihydrate(Citric Acid, Sodium Salt; Sodium Citrate, Dihydrate)
USBioAnalyzedC
6H
5Na
3O
7•2H
2O
Formula Weight: 294.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5% (dry basis)Identity A: By IRIdentity B: Passes testLoss on Drying: 10.0 - 13.0%Tartrate: Passes testAlkalinity: Passes testHeavy Metals (Pb): ≤ 10 ppmCAS #6132-04-3
13735 500gm 1kg 5kg
Bioc
hem
ical
sBiochemicals
�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
L-CitrullineNH
2CONH(CH
2)3CH(NH
2)COOH
Formula Weight: 175.19PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +24.5° to +26.5° (c=8 in 6N HCl at 20°C)
Loss on Drying: ≤ 0.3%Heavy Metals: ≤ 20 ppmArsenic (As): ≤ 2 ppmChloride (Cl): ≤ 0.02%Sulfate (SO
4): ≤ 0.02%
CAS #372-75-8
13745 5gm 25gm 100gm 1kg
CMPSee Cytidine-5'-Monophosphate, page 212
Cocarboxylase(Thiamine Pyrophosphate Chloride)
C12
H19
CIN4O
7P
2S
Formula Weight.: 460.77PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 93.0% (dry basis)Identity: By IRLoss on Drying (130°C): ≤ 1.5%Chloride (Cl): 7.5 - 7.9%Sulfate (SO
4): ≤ 0.01%
Heavy Metals (Pb): ≤ 10 ppmCAS #154-87-0
13765 5gm 10gm 25gm 100gm
Cocoa Butter
13770 1kg
Cod Liver OilPRODUCT SPECIFICATIONSForm: Amber liquidVitamin A: ≥ 850 USP Units/gmVitamin D: ≥ 85 USP Units/gmIodine Value: 145 - 180Saponification Value: 180 - 192CAS #8001-69-2
13768 1qt 1ga
CodecarboxylaseSee Pyridoxal-5'-Phosphate, page 280
Coenzyme A, Free Acid, TrihydrateC
21H
36N
7O
16P
3S•3H
2O
Formula Weight: 821.58PRODUCT SPECIFICATIONSForm: Lyophilized powderAssay: ≥ 75% as reduced Co-A•3H
2O
Spectral Ratios (pH 7.5): A
250/A
260: 0.78 ± 0.03
A280
/A260
: 0.16 ± 0.03Moisture (KF): ≤ 8%Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°CCAS #85-61-0
13787 10mg 25mg 50mg 100mg 250mg 500mg 1gm
Coenzyme A, Dilithium SaltC
21H
34N
7O
16P
3SLi
2•nH
2O
Formula Weight: 779.40•nH2O
PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Enzymatic): ≥ 75%Lithium (Li): ≤ 2%Moisture (KF): ≤ 8%Spectral Ratios (pH 7.5): A
250/A
260: 0.78 ± 0.03
A280
/A260
: 0.16 ± 0.03Storage: -20°C
13784 50mg 100mg 500mg 1gm
Coenzyme A, Trilithium Salt, DihydrateC
21H
33N
7O
16P
3SLi
3•2H
2O
Formula Weight: 821.36PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Enzymatic): ≥ 75%Spectral Ratios (pH 7.5): A
250/A
260: 0.78 ± 0.03
A280
/A260
: 0.16 ± 0.03Lithium (Li): 2.5 ± 1.5%Moisture (KF): ≤ 8%Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°CCAS #18439-24-2
13785 10mg 25mg 50mg 100mg 250mg 500mg 1gm
Coenzyme ISee β-Nicotinamide Adenine Dinucleotide,
page 262
Coenzyme IISee Nicotinamide Adenine Dinucleotide
Phosphate, page 263
ColchicineC
22H
25NO
6
Formula Weight: 399.44PRODUCT SPECIFICATIONSForm: White to pale yellow powderIdentity: By IRAssay: 94.0 - 101.0% (dry basis)[α]25
D : -240° to -250° (c=1 in EtOH, dry basis)
Moisture (KF): ≤ 2.0%Limit of Colchiceine: Passes testLimit of Ethyl Acetate: Passes testChromatographic Purity: Passes testO.V.I.: Meets requirementCAS #64-86-8
13805 500mg 1gm 5gm
Biochemicals
Biochemicals
�5w w w . u s b w e b . c o m
CollagenSource: Bovine Achilles TendonPRODUCT SPECIFICATIONSForm: Lyophilized shredded powderIdentity: By IRPurified by the method of Einbinder and Schubert,
(1951) J. Biol. Chem., 188, 355.Storage: 4°CCAS #9007-34-5
13815 1gm 5gm 10gm
CollagenSource: Calf SkinPRODUCT SPECIFICATIONSForm: Solution in dilute (0.075M) acetic acid with
0.01% thimerosal as a preservative, pH 3.7.Collagen concentration: Approximately 10 mg/mlStorage: 4°C
13813 10ml 25ml 100ml
CollagenSource: Calf SkinPRODUCT SPECIFICATIONSProtein: 6-10 mg/mlDiluent: 0.075M Sodium Citrate, pH 3.7,
containing 0.01% Sodium Azide as a preservative.
Storage: 4°C
13814 10ml 25ml 100ml
Collagen, Type IISource: Calf Articular Joint
13817 10mg 25mg
Collagenase, Type I(E.C.3.4.24.3)Source: Clostridium histolyticumDescription: Contains a mixture of collagenase,
clostripain, trypsin, caseinase and other lytic enzymes.
PRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 125 units/mg powder (dry basis) ≥ 0.15 Wunsch units/mg powder (dry basis)Unit Definition: One unit liberates 1 µmol of
L-Leucine equivalents from collagen in 5 hr at 37°C, pH 7.5. One unit is equal to 0.0012 Wunsch units.
Contaminating Activities: Caseinase: 200 - 1000 units/mg Clostripain: 1.0 - 3.5 units/mgStorage: 4°C, protect from moisture.CAS #9001-12-1
13820 100mg 1gm 5gm
Compact Reaction Columns(CRC)
With screw-on lid, Luer-lock lid, stopper and filter insertion tools.
Upper and lower filters with a pore size appropriate to the customer’s needs must be purchased separately.
50 columns/pk
13928 1pk
Compact Reaction Columns(CRC) without screw on lids
Stopper and filter insertion tools included.Upper and lower filters with a pore size
appropriate to the customer’s needs must be purchased separately.
100 columns/pk
13929 1pk
CRC Lower Filters(2.7 mm)
10 µm pore size50 filters/pk
13911 1pk
CRC Lower Filters(2.7 mm)
35 µm pore size50 filters/pk
13912 1pk
CRC Lower Filters(2.7 mm)
90 µm pore size50 filters/pk
13913 1pk
CRC Upper Filters(6.8 mm)
10 µm pore size50 filters/pk
13914 1pk
CRC Upper Filters(6.8 mm)
35 µm pore size50 filters/pk
13918 1pk
CRC Upper Filters(6.8 mm)
90 µm pore size50 filters/pk
13919 1pk
CRC Leur-Lock AdaptorFor connection to tubing with 1 m Teflon® tube.
13926 1adaptor
Concanavalin ASee Canavalia Ensiformis, page 202
Coomassie® StainsSee Brilliant Blue G & R, page 199
Corn-Ground Yellow Maize
13845 25lb 100lb
Bioc
hem
ical
sBiochemicals
�6 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Corn OilWith 0.01% BHA and 0.01% BHT addedCAS #8000-30-7
13851 1lb 5lb 25lb
Corn Oil-Tocopherol StrippedPRODUCT SPECIFICATIONSForm: Clear, yellow viscous liquidIdentity: By IRRefractive Index: 1.4730 - 1.4760Peroxide Value: ≤ 8 meq/kgTotal Tocopherols: ≤ 25 µg/gmCAS #8001-30-7
13852 400gm
Corn StarchSee Starch-Corn, page 290
Corticosterone(Reichstein’s Substance H)
C21
H30
O4
Formula Weight: 346.46PRODUCT SPECIFICATIONSForm: Off-white powderAssay (HPLC): ≥ 95%Identity: By IRMelting Point: 180 - 182°CStorage: 4°CCAS #50-22-6
13860 100mg 500mg 1gm
Cottonseed OilCAS #8001-29-4
13871 5lb 25lb
CozymaseSee Nicotinamide Adenine Dinucleotide, page 262
c-Reactive Protein, Recombinant(r-hCRP)
Source: Human recombinant from E. coliPRODUCT SPECIFICATIONSForm: Suspension in 20mM Tris (pH 7.5), 2mM
CaCl2, 0.14M NaCl, containing 0.05% NaN
3
Concentration: 1 mg/mlPurity: Single band at about 23k, as determined
by SDS-PAGE under reducing conditionsMolecular Weight: 115,000 which corresponds
to the pentamer structure of 23,000 monomer determined by amino acid sequence
Storage: +4°C , Do not freeze
26001 1mg 5mg
Creatine Monohydrate(Methylguanido-Acetic Acid)
NHC(NH2)N(CH
3)CH
2COOH•H
2O
Formula Weight: 149.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97.0%Identity: By IRChromatography (TLC): HomogeneousMoisture (KF): ≤ 13.0%Residue on Ignition: ≤ 0.1%Heavy Metals(Pb): ≤ 50 ppmCAS #6020-87-7
13910 25gm 100gm 1kg
Creatine Kinase(E.C.2.7.3.2)Source: Rabbit MuscleUnit Definition: One unit phosphorylates 1 µmol of
creatine/min at 25°C, pH 9.0.CAS #9001-15-4
13915 100mg 1gm
Creatine Kinase MB, Recombinant(r-hCK-MB)
Source: Human recombinant from E. coliPRODUCT SPECIFICATIONSForm: Suspension in 20mM Tris-HCl (pH 8.5),
150mM NaCl, 10mM β-ME, 50% glycerol and 0.1% NaN
3.
Protein Concentration: ≥ 2 mg/mlPurity (SDS-PAGE): Single bandSpecific Activity: ≥ 500 units/mg proteinStorage: -20°C
26009 100units
Creatine Kinase MM, Recombinant(r-hCK-MM)
Source: Human recombinant from E. coliPRODUCT SPECIFICATIONSForm: Suspension in 20mM PIPES (pH 7.0),
150mM NaCl, 10mM β-ME, 10mM MgOAc, 50% glycerol and 0.1% NaN
3.
Protein Concentration: ≥ 10 mg/mlPurity (SDS-PAGE): Single bandSpecific Activity: ≥ 500 units/mg proteinStorage: -20°C
26010 100units
Creatine Phosphate, Disodium Salt, Tetrahydrate(Phosphocreatine)
C4H
8N
3O
5PNa
2•4H
2O
Formula Weight: 327.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Chromatography (TLC): HomogeneousIdentity: By IRMoisture (KF): 23.5 ± 2.5%Storage: 4°CCAS #922-32-7
13920 1gm 5gm 25gm
CreatinineC
4H
7N
3O
Formula Weight: 113.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 102%Identity: By IRLoss on Drying: ≤ 3.0%Residue on Ignition: ≤ 0.2%CAS #60-27-5
13930 25gm 100gm 1kg
o-Cresolphthalein ComplexoneC
32H
32N
2O
12
Formula Weight: 636.60CAS #2411-89-4
13937 5gm 25gm
Biochemicals
Biochemicals
�7w w w . u s b w e b . c o m
Cresol Red, Sodium SaltPRODUCT SPECIFICATIONSForm: Red-brown powderIdentity: By IRVisual Transition Interval: pH 0.2 - Red pH 1.8 - Orange-yellow pH 7.0 - Yellow pH 8.8 - Purplish-redCAS #62625-29-0
13940 5gm 10gm 25gm
Crystal Violet(C.I.42555)C
25H
30ClN
3
Formula Weight: 407.98CAS #548-62-9
13946 25gm 100gm
CTABSee Cetyl Trimethyl Ammonium Bromide, page 205
CTPSee Cytidine-5'-Triphosphate, page 213
Cupric Sulfate PentahydrateACS Reagent GradeCuSO
4•5H
2O
Formula Weight: 249.68PRODUCT SPECIFICATIONSForm: Blue crystalline powderAssay: 98.0 - 102.0%Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.001%Nitrogen Compounds (N): ≤ 0.002%Calcium (Ca): ≤ 0.005%Iron (Fe): ≤ 0.003%Nickel (Ni): ≤ 0.005%Potassium (K): ≤ 0.01%Sodium (Na): ≤ 0.02%
13839 500gm 2.5kg
CyanocobalaminSee Vitamin B
12, page 308
Cyclic GMPSee Guanosine Cyclic Monophosphate, page 237
-CyclodextrinC
36H
60O
30
Formula Weight: 972.84PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (HPLC): ≥ 98.0%[α]20
D : +147° to +152° (c=1 in H
2O)
Loss on Drying: ≤ 12%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.018%Arsenic (As): ≤ 2 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #10016-20-3
13979 25gm
β-CyclodextrinC
42H
7OO
35
Formula Weight: 1134.98PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (HPLC): ≥ 98.0%[α]20
D : +160° to +164° (c=1 in H
2O)
Loss on Drying: ≤ 14%Residue on Ignition: ≤ 0.1%CAS #7585-39-9
13980 100gm
D-CycloserineC
3H
6N
2O
2
Formula Weight: 102.09PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderAssay: ≥ 98.0% (dry basis) ≥ 95.0% (as is)Identity: By IR[α]25
D : +108° to +114° (c=5 in 2N NaOH)
pH (10%, H2O): 5.7 - 6.3
Loss on Drying: ≤ 1.0%Moisture (KF): ≤ 3.0%Mode of Action: Inhibits cell wall synthesis.
D-Cycloserine prevents the formation of D-Alanine from L-Alanine and the formation of peptide bonds with D-Alanine.
Stock Solution: 10 mg/ml in 0.1M sodium phosphate buffer,
pH 8.0 This solution is very unstable. Make
immediately prior to use.Working Concentration: 200 µg/mlStorage: 4°C, in tight containersCAS #68-41-7
14000 1gm 5gm 10gm 25gm
DL-Cysteic AcidCH
2(SO
3H)CH(NH
2)COOH•H
2O
Formula Weight: 187.17CAS #3024-83-7
14009 1gm 5gm
D-Cysteine, HydrochlorideCH
2(SH)CH(COOH)NH
2•HCl•H
2O
Formula Weight: 175.63CAS #32443-99-5
14015 250mg 1gm
DL-Cysteine, HydrochlorideCH
2(SH)CH(COOH)NH
2•HCl•H
2O
Formula Weight: 175.63CAS #10318-18-0
14020 1gm 5gm 10gm
L-Cysteine, Free BaseHS-CH
2CH(NH
2)COOH
Formula Weight: 121.16PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98% (dry basis)Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +7.0° to +9.0° (c=8, 1N HCl)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.2%Heavy Metals (Pb): ≤ 10 ppmArsenic (As
2O
3): ≤ 2 ppm
Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 30 ppmSulfate (SO
4): ≤ 0.03%
CAS #52-90-4
14030 25gm 100gm 1kg
Bioc
hem
ical
sBiochemicals
�8 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
L-Cysteine, Hydrochloride, Monohydrate
HSCH2CH(NH
2)COOH•HCl•H
2O
Formula Weight: 175.63PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IRChromatography: Homogeneous[α]25
D : +5.7° to +6.8° (c=8 in in 6N HCl,
dry basis)Loss on Drying: 8.0 - 12.0%Residue on Ignition: ≤ 0.1%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 10 ppmArsenic (As): ≤ 1.5 ppmChloride (Cl): 19.8 - 20.8%Iron (Fe): ≤ 10 ppmCAS #7048-04-6
14035 25gm 100gm 1kg 5kg
D-Cystine[-SCH
2CH(NH
2)COOH]
2
Formula Weight: 240.30PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IRChromatography (TLC): Homogeneous[α]20
D : +211° to +219° (c=2 in 1N HCl)
CAS #349-46-2
14040 250mg 500mg 1gm
DL-Cystine[-SCH
2CH(NH
2)COOH]
2
Formula Weight: 240.30PRODUCT SPECIFICATIONSForm: White powderIdentity: By IRPurity: ≥ 99%CAS #923-32-0
14045 1gm 5gm
L-CystineCP Grade[-SCH
2CH(NH
2)COOH]
2
Formula Weight: 240.30PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IR[α]20
D : -215° to -225° (c=2 in 1N HCl)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 20 ppmLead (Pb): ≤ 10 ppmCAS #56-89-3
14050 50gm 100gm 1kg 5kg
L-Cystine, DihydrochlorideC
6H
12N
2O
4S
2•2HCl
Formula Weight: 313.22Water Soluble. Suggested for use in the
preparation of tissue culture media.PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Chromatography (TLC): HomogeneousIdentity: By IR[α]20
D : -215° to -225°, dry basis, as diHCl;
(c=2 in 1N HCl)Loss on Drying: ≤ 1.0%Solubility (10 mg/100 ml): Passes test
14051 100gm
L-Cystine, Disodium Salt, Monohydrate[-SCH
2CH(NH
2)COO]
2•2Na•H
2O
Formula Weight: 302.28PRODUCT SPECIFICATIONSForm: White to off-white powderIdentity: By IRChromatography (TLC): HomogeneouspH (5%, H
2O): 10.5 ± 0.5
Solubility: Passes test[α]20
D : -181° to -191° (c=2 in 1N HCl)
Loss on Drying: ≤ 6% (110°C, 4 hrs)CAS #64704-23-0
14021 25gm 100gm 1kg
Cytidine, Free BaseC
9H
13N
3O
5
Formula Weight: 243.22PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV): ≥ 97.5%Identity: By IRλ
max (pH 1.0): 280 nm ± 2 nm
Spectral Ratios (pH 1.0): A
250/A
260: 0.45 ± 0.02
A280
/A260
: 2.10 ± 0.05Loss on Drying: ≤ 1.0%Reference Constants:λ
max (pH 7, NRC ): 271 nm
εmax
(pH 7, NRC ): 9.1 x 103
ε260
(pH 7, DBR ): 7.55 x 103
CAS #65-46-3
14060 5gm 10gm 25gm 100gm
Cytidine-5'-Monophosphate, Free Acid(CMP)
C9H
14N
3O
8P
Formula Weight: 323.20PRODUCT SPECIFICATIONSForm: White crystalline powderPurity: ≥ 98%Loss on Drying: ≤ 6%Reference Constants:λ
max (pH 7, NRC ): 271 nm
εmax
(pH 7, NRC ): 9.1 x 103
ε260
(pH 7, DBR ): 7.4 x 103
Storage: -20°C, protect from moisture.CAS #63-37-6
14113 1gm 5gm 10gm
Cytidine-5'-Monophosphate, Disodium Salt(CMP)
C9H
12N
3O
8PNa
2•7H
2O
Formula Weight: 493.27Reference Constants:λ
max (pH 7, NRC ): 271 nm
εmax
(pH 7, NRC ): 9.1 x 103
ε260
(pH 7, DBR ): 7.4 x 103
Storage: 4°CCAS #6757-06-8
14110 1gm 5gm 10gm 100gm
Biochemicals
Biochemicals
�9w w w . u s b w e b . c o m
Cytidine 5'-Triphosphate, 100mM Solution(CTP)
ULTRAPUREC
9H
12N
3O
14P
3Na
4
Formula Weight: 571.08Form: 100mM aqueous solution, pH 7.5Storage: -20°C
77242 25µmol(250µl)
Cytidine-5'-Triphosphate, Disodium Salt, Dihydrate(CTP)
C9H
14N
3O
14P
3Na
2•2H
2O
Formula Weight: 527.13 (anhydrous)Formula Weight: 563.16 (dihydrate)PRODUCT SPECIFICATIONSForm: White powderAssay (Total UV, pH 2.0): ≥ 98.0%λ
max: 280 nm ± 1 nm
εmax
: (pH 2.0): [13.0 ± 0.3] x 103
Spectral Ratios (pH 2.0): A
250/A
260: 0.45 ± 0.05
A280
/A260
: 2.10 ± 0.07Moisture (KF): ≤ 12.0%Reference Constants:λ
max (pH 7, NRC ): 271 nm
εmax
(pH 7, NRC ): 9.1 x 103
ε260
(pH 7, DBR ): 7.4 x 103
Storage: -20°C, protect from moisture.CAS #81012-87-5
14121 25mg 100mg 500mg 1gm
Cytochrome CSource: Horse HeartPRODUCT SPECIFICATIONSForm: Reddish or dark brown crystalline powderAssay: ≥ 90.0% (dry basis)Identity: Passes testpH (1%, H
2O): 5.0 - 7.0
Moisture (KF): ≤ 6.0%Iron (Fe): 0.40 to 0.50%Residue on Ignition: ≤ 1.5%Solubility (10%, H
2O): Clear dark red solution
Storage: -20°C
14102 100mg 500mg 1gm
CytosineC
4H
5N
3O
Formula Weight: 111.10Reference Constants:λ
max (pH 7, NRC ): 267 nm
εmax
(pH 7, NRC ): 6.1 x 103
ε260
(pH 7, DBR ): 2.6 x 103
CAS #71-30-7
14130 5gm 10gm 25gm 100gm
Cytosine Arabinoside
ULTRAPUREC
9H
13N
3O
5
Formula Weight: 243.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5% (dry basis)Identity: By IR[α]20
D : +157° ± 3° (c=1, H
2O)
λmax
: 281 nm ± 2 nmε
max: (13.5 + 0.4) x 103
Melting Point: 215 ± 5°CLoss on Drying: ≤ 1.0%Storage: 4°C
14138 500mg 1gm 10gm
n-Decyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate
See Sulfobetaine 10 (SB3-10), page 292
DAPI(4',6-Diamidino-2-Phenylindole Dihydrochloride)
C16
H15
N5•2HCl
Formula Weight: 350.25PRODUCT SPECIFICATIONS:Form: Yellow powderAssay (HPLC): ≥ 98%Identity: By IRSolubility (1%, H
2O): Clear
Nitrogen: ≥ 18.0%Applications:•Cytochemical technique for demonstration of
DNA in cells infected with mycoplasmas and viruses(1)
•Interaction of DAPI with synthetic polynucleotides(2)
•DAPI-stained cells can be fixed for high resolution flow cytometry(3)
•Reverse fluorescent chromosome banding by double-staining with chromomycin and DAPI(4)
•Binding of DAPI to GC and mixed sequences in DNA with an intercalation of a groove-binding molecule(5)
Notes: DAPI is an excellent dye for the staining of DNA. Originally, only the specific binding to AT-base pairs without an intercalation was known(2). Later on, the intercalation into GC-base pairs was shown. The most popular application of DAPI is its use as a reagent to detect mycoplasma or virus DNA (e.g. vaccinia infection or ‘unwanted’ viral contamination of cell culture cells) in cell culture. Reference 1 describes a simple procedure to stain DNA with DAPI: Grow cells on a coverslip in a cell culture dish. Wash the coverslip once with PBS. Incubate the cells on the coverslip at 37°C for 15 - 30 minutes in 0.1 µg DAPI/ml PBS. Wash the coverslip in PBS and put it up-side-down on a slide. Examine the cells under a microscope (excitation: 365 nm; emission maximum at 450 nm). Prolonged incubation with DAPI increases the nuclear fluorescence; shorter incubation time leads to a weaker nuclear staining, which facilitates the examination of the cytoplasmic fluorescence.
DAPI can be dissolved in water, PBS, 5mM Tris-HCl (pH 7.6)/8mM NaCl, citrate or phosphate buffer(3). The solubility is approximately 2.5%. Stock solutions are prepared at concentrations ranging from 1-2 mg/ml and the working dilution is 1:1000. DAPI solution is stable at room temperature for 1 - 2 weeks(3), at 4°C up to 6 months, and at -20°C for 6 - 12 months. DAPI has hydrolyzed if the solution becomes turbid. DAPI bleaches quickly in contact with light, even though it is quite stable against UV-light.
References:1. Russel, W. C., Newman, C. and Williamson, D.
H. (1975) Nature 253, 461-462.2. Kapuscinski, J. and Szer, W. (1979) Nucleic
Acids Res. 6, 3519-3534. 3. Otto, F. (1990) Methods Cell Biol. 33, 105-
110.4. Schweizer, D. (1976) Chromosoma. 29, 307-
324.5. Wilson, W. D., Tanious, F. A., Barton, H. J.,
Jones, R. L., Fox, K., Wydra, R. L. and Strekowski, L. (1990) Biochem. 11, 8452-8461.
Storage: 4°CCAS #28718-90-3
14564 10mg 50mg
Bioc
hem
ical
sBiochemicals
50 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Denhardt’s Solution, 50X
ULTRAPUREMB GradeForm: 50X solution of 1% acetylated bovine
serum albumin (0.2 µm filtered), 1% Ficoll (autoclaved before use) and 1% PVP-90 (autoclaved before use). Dispensed into autoclaved bottles.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testStorage: -20°CApplication: •For use as a blocking reagent in nucleic acid
hybridization techniquesFormamide Hybridization Buffer: 50%
Formamide, 5X SSC, 0.5% SDS, 5X Denhardt’s Solution, 100 µg/ml sonicated fish sperm DNA
Reagent Amount for every 100 mlFormamide* 50 ml20X SSC* 25 ml20% SDS* 2.5 ml50X Denhardt’s Solution* 10 ml10 mg/ml fish DNA 1.0 mlWater* up to 100 ml*Denotes Ultrapure Reagent available from USB.Reference:1. Sambrook, J., Fritsch, E. F. and Maniatis, T.
(1989) Molecular Cloning, 2nd Edition, Cold Spring Harbor Laboratory.
70468 50ml
2'-DeoxyadenosineC
10H
13N
5O
3•H
2O
Formula Weight: 269.26PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Total UV): ≥ 98%Identity: By IRλ
max: 258-260 nm
Spectral Ratios: A
250/A
260: 0.77 ± 0.04
A280
/A260
: 0.15 ± 0.05Moisture (KF): ≤ 8.0%Reference Constants:λ
max (pH 7, NRC ): 260 nm
εmax
(pH 7, NRC ): 14.9 x 103
ε260
(pH 7, DBR ): 15.2 x 103
CAS #16373-93-6
14235 1gm 5gm 25gm
2'-Deoxyadenosine-5'-Monophosphate, Free Acid(dAMP)
C10
H14
N5O
6P•nH
2O
Formula Weight: 331.22•nH2O
Reference Constants:λ
max (pH 7, DBR ): 259 nm
εmax
(pH 7, DBR ): 15.4 x 103
ε260
(pH 7, DBR ): 15.3 x 103
Storage: 4°CCAS #653-63-4
14225 100mg 250mg
2'-Deoxyadenosine-5'-Triphosphate, Lithium Salt, 100mM Solution(dATP)
ULTRAPUREC
10H
12N
5O
12P
3Li
4
Formula Weight: 514.87PRODUCT SPECIFICATIONSForm: 100mM Solution, pH 7.1Assay (HPLC): ≥ 98.0%pH: 7.0 ± 0.2λ
max: 260 nm ± 1 nm
Spectral Ratios: A
250/A
260: 0.78 ± 0.02
A280
/A260
: 0.15 ± 0.01Storage: -20°C
77302 25µmol(250µl)
2'-Deoxyadenosine-5'-Triphosphate, Sodium Salt, 100mM Solution(dATP)
ULTRAPUREC
10H
12N
5O
12P
3Na
4
Formula Weight: 579.11PRODUCT SPECIFICATIONSForm: 100mM aqueous solutionAssay (HPLC): ≥ 99%pH: 7.5 ± 0.2λ
max: 259 nm ± 1 nm
Spectral Ratios: A
250/A
260: 0.80 ± 0.03
A280
/A260
: 0.12 ± 0.02Functional Tests: Meets or exceeds criteria for
high quality sequencing. Functionally tested in long PCR.
Storage: -20°C
77102 25µmol(250µl) 100µmol(1ml) 500µmol(5ml)
2'-Deoxyadenosine-5'-Triphosphate, Disodium Salt(dATP)
C10
H14
N5O
12P
3Na
2•nH
2O
Formula Weight: 535.15•nH2O
PRODUCT SPECIFICATIONSForm: White powderAssay (Total UV, pH 7.0): 97.0 - 103.0%
(dry basis)Assay (HPLC): ≥ 95% (as is)λ
max (pH 7.0): 259 nm ± 1 nm
εmax
(pH 7.0): [15.2 ± 0.5] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.78 ± 0.03
A280
/A260
: 0.15 ± 0.02Note: Water of hydration may vary up to a
trihydrate.Reference Constants:λ
max (pH 7, DBR ): 259 nm
εmax
(pH 7, DBR ): 15.3 x 103
ε260
(pH 7, DBR ): 15.4 x 103
Storage: -20°CCAS #1927-31-7
14245 10mg 25mg 100mg 1gm
Deoxycholic Acid, Sodium SaltSodium Deoxycholate
C24
H39
NaO4
Formula Weight: 414.55PRODUCT SPECIFICATIONSForm: White to off-white powderAssay (HPLC): ≥ 98% (dry basis)Identity: By IRLoss on Drying (4 hr, 140°C, vacuum): ≤ 6%Sodium Cholate: ≤ 2%CAS #302-95-4
21026 25gm 100gm
2'-Deoxycytidine, Free BaseC
9H
13N
3O
4
Formula Weight: 227.22Reference Constants:λ
max (pH 7, NRC ): 271 nm
εmax
(pH 7, NRC ): 9.0 x 103
ε260
(pH 7, DBR ): 7.4 x 103
CAS #951-77-9
14260 500mg 1gm 5gm
Biochemicals
Biochemicals
51w w w . u s b w e b . c o m
2'-Deoxycytidine, HydrochlorideC
9H
13N
3O
4•HCl
Formula Weight: 263.68PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 100 ± 3% (dry basis)Identity: By IRλ
max: 280 ± 2 nm
Spectral Ratios (pH 1.0): A
250/A
260: 0.43 ± 0.03
A280
/A260
: 2.10 ± 0.10Loss on Drying (105°C, 4 hrs): ≤ 1%Heavy Metals: ≤ 10 ppmReference Constants:λ
max (pH 7, NRC ): 271 nm
εmax
(pH 7, NRC ): 9.0 x 103
ε260
(pH 7, DBR ): 7.4 x 103
CAS #3992-42-5
14270 500mg 1gm 5gm 10gm 25gm
2'-Deoxycytidine, Hydrochloride, Synthetic
C9H
13N
3O
4•HCI
Formula Weight: 263.68PRODUCT SPECIFICATIONSForm: White crytalline powderAssay (Total UV): ≥ 98% (dry basis)Identity: By IRλ
max: 280 ± 2 nm
εmax
(pH 1.0): 13.2 x 103 ± 2%Spectral Ratios (pH 1.0): A
250/A
260: 0.43 ± 0.03
A280
/A260
: 2.10 ± 0.05Moisture (KF): ≤ 1%Heavy Metals: ≤ 10 ppmCAS #3992-42-5
14284
2'-Deoxycytidine-5'-Monophosphate, Disodium Salt(dCMP)
C9H
12N
3O
7PNa
2•nH
2O
Formula Weight: 351.16•nH2O
Reference Constants:λ
max (pH 7, NRC ): 270 nm
εmax
(pH 7, NRC ): 9.1 x 103
ε260
(pH 7, DBR ): 7.4 x 103
Storage: 4°CCAS #13085-50-2
14275 100mg 250mg 1gm
2'-Deoxycytidine-5'-Triphosphate, Lithium Salt, 100mM Solution(dCTP)
ULTRAPUREC
9H
12N
3O
13P
3Li
4
Formula Weight: 409.88PRODUCT SPECIFICATIONSForm: 100mM solution, pH 7.1Assay (HPLC): ≥ 98.0%pH: 7.0 ± 0.2λ
max: 272 nm ± 1 nm
Spectral Ratios: A
250/A
260: 0.82 ± 0.02
A280
/A260
: 0.99 ± 0.02 A
290/A
260: 0.30 ± 0.02
Storage: -20°C
77304 25µmol(250µl)
2'-Deoxycytidine-5'-Triphosphate, Sodium Salt, 100mM Solution(dCTP)
ULTRAPUREC
9H
12N
3O
13P
3Na
4
Formula Weight: 555.08PRODUCT SPECIFICATIONSForm: 100mM aqueous solutionpH 7.5: 7.5 ± 0.2Assay (HPLC): ≥ 99%λ
max (pH 2.0): 280 nm ± 1 nm
Spectral Ratios (pH 2.0): A
250/A
260: 0.45 ± 0.03
A280
/A260
: 2.10 ± 0.15 A
290/A
260: 1.60 ± 0.10
Functional Test: Meets or exceeds criteria for high quality sequencing. Functionally tested in long PCR.
Storage: -20°C
77104 25µmol(250µl) 100µmol(1ml) 500µmol(5ml)
2'-Deoxycytidine-5'-Triphosphate, Trisodium Salt(dCTP)
C9H
13N
3O
13P
3Na
3•nH
2O
Formula Weight: 533.10•nH2O
PRODUCT SPECIFICATIONSForm: White powderAssay (Total UV, pH 2.0): ≥ 95.0% (dry basis)Assay (HPLC): ≥ 95.0% (as is)λ
max (pH 2.0): 280 nm ± 1 nm
εmax
(pH 2.0): [13.0 ± 0.6] x 103
Spectral Ratios (pH 2.0): A
250/A
260: 0.45 ± 0.03
A280
/A260
: 2.10 ± 0.15Reference Constants:λ
max (pH 7, DBR ): 272 nm
εmax
(pH 7, DBR ): 9.1 x 103
ε260
(pH 7, DBR ): 7.4 x 103
Storage: -20°CCAS #102783-51-7
14280 10mg 25mg 100mg 250mg
Deoxycytidylic AcidSee 2'-Deoxycytidine-5'-Monophosphate,
page 215
2-Deoxy-D-GlucoseC
6H
12O
5
Formula Weight: 164.16PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (HPLC): ≥ 98%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +46° ± 2° (c=1 in H
2O, 24 hr)
Moisture (KF): ≤ 1.0%CAS #154-17-6
14290 1gm 5gm 25gm
Bioc
hem
ical
sBiochemicals
5� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
2'-DeoxyguanosineC
10H
13N
5O
4•H
2O
Formula Weight: 285.26PRODUCT SPECIFICATIONSForm: White granular powderAssay (HPLC): ≥ 97%Identity: By IRChromatography (TLC): Homogeneousλ
max: 253 ± 2 nm
εmax
: 12800-14300Spectral Ratios (pH 7.0): A
250/A
260: 1.09 - 1.21
A280
/A260
: 0.63 - 0.71Moisture (KF): ≤ 9%Reference Constants:λ
max (pH 7, NRC ): 254 nm
εmax
(pH 7, NRC ): 13.0 x 103
CAS #961-07-9
14300 1gm 5gm 25gm 100gm
2'-Deoxyguanosine, Monohydrate Synthetic
C10
H13
N5O
4•H
2O
Formula Weight: 285.26PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98% (dry basis)Chromatography (TLC): HomogeneousIdentity: By IRλ
max (pH 7.0): 253 ± 2 nm
εmax
(pH 7.0): (13.7 x 103) ± 4%Spectral Ratios (pH 7): A
250/A
260: 1.12 - 1.18
A280
/A260
: 0.65 - 0.69Moisture (KF): ≤ 7.0%CAS #961-07-9
14304
2'-Deoxyguanosine-5'-Diphosphate, Disodium Salt(dGDP)
C10
H13
N5O
10P
2Na
2•nH
2O
Formula Weight: 471.16•nH2O
Reference Constants:λ
max (pH 7, NRC ): 253 nm
εmax
(pH 7, NRC ): 13.8 x 103
ε260
(pH 7, DBR ): 11.8 x 103
Storage: -20°CCAS #102783-74-4
14305 10mg 50mg 100mg
2'-Deoxyguanosine-5'-Monophosphate, Disodium Salt(dGMP)
C10
H12
N5O
7PNa
2•nH
2O
Formula Weight: 391.18•nH2O
Reference Constants:λ
max (pH 7, NRC ): 253 nm
εmax
(pH 7, NRC ): 13.8 x 103
ε260
(pH 7, DBR ): 11.8 x 103
Storage: -20°CCAS #33430-61-4
14310 100mg 250mg 1gm
2'-Deoxyguanosine-5'-Triphosphate Lithium Salt, 100mM Solution(dGTP)
ULTRAPUREC
10H
12N
5O
13P
3Li
4
Formula Weight: 530.92PRODUCT SPECIFICATIONSForm: 100mM solution, pH 7.1Assay (HPLC): ≥ 95.0%pH: 7.0 ± 0.2λ
max: 252 nm ± 1 nm
Spectral Ratios: A
250/A
260: 1.16 ± 0.02
A280
/A260
: 0.67 ± 0.02Storage: -20°C
77306 25µmol(250µl)
7-deaza-2'-Deoxyguanosine-5'-Triphosphate, Lithium Salt, 10mM Solution(7-deaza-dGTP)
C11
H15
N4O
13P
3Li
2
Formula Weight: 518.10Form: 10mM aqueous solutionAssay (FPLC): 85%pH: 7.0λ
max: 259 nm ± 1 nm
Functional Tests: Meets or exceeds criteria for high quality sequencing.
Storage: -20°C
70065 2µmol
2'-Deoxyguanosine-5'-Triphosphate, Sodium Salt, 100mM Solution(dGTP)
ULTRAPUREC
10H
12N
5O
13P
3Na
4
Formula Weight: 595.11PRODUCT SPECIFICATIONSForm: 100mM aqueous solutionAssay (HPLC): ≥ 99%pH: 7.5 ± 0.2λ
max: 252 nm ± 1 nm
Spectral Ratios (pH 7.5): A
250/A
260: 1.18 ± 0.04
A280
/A260
: 0.67 ± 0.03 A
290/A
260: 0.28 ± 0.03
Functional Test: Meets or exceeds criteria for high quality sequencing. Functionally tested in long PCR.
Storage: -20°C
77106 25µmol(250µl) 100µmol(1ml) 500µmol(5ml)
2'-Deoxyguanosine-5'-Triphosphate, Trisodium Salt(dGTP)
C10
H13
N5O
13P
3Na
3•nH
2O
Formula Weight: 573.13•nH2O
PRODUCT SPECIFICATIONSForm: White powderAssay (Total UV, pH 7.5): ≥ 96.0% (dry basis)Assay (HPLC): ≥ 95.0% (as is)λ
max: (pH 7.5) 253 nm ± 1 nm
εmax
: (pH 7.5) (13.7 ± 0.6) x 103
Spectral Ratios (pH 7.5): A
250/A
260: 1.18 ± 0.04
A280
/A260
: 0.67 ± 0.03Reference Constants:λ
max (pH 7, DBR ): 252 nm
εmax
(pH 7, DBR ): 13.7 x 103
ε260
(pH 7, DBR ): 11.9 x 103
Storage: -20°CCAS #93919-41-6
14315 10mg 25mg 100mg 250mg
Biochemicals
Biochemicals
53w w w . u s b w e b . c o m
2'-DeoxyinosineC
10H
12N
4O
4
Formula Weight: 252.23Reference Constants:λ
max (pH 7, NRC ): 249 nm
εmax
(pH 7, NRC ): 12.8 x 103
Storage: -20°CCAS #890-38-0
14320 250mg 1gm 5gm
2'-Deoxyinosine-5'-Triphosphate, Sodium Salt, 100mM Solution(dITP)
ULTRAPUREC
10H
12N
4O
13P
3Na
4
Formula Weight: 581.10PRODUCT SPECIFICATIONSForm: 100mM aqueous solution, pH 7.5Assay (HPLC): ≥ 99%λ
max: 249 nm ± 1 nm
Spectral Ratios (pH 7.5): A
250/A
260: 1.70 ± 0.10
A280
/A260
: 0.23 ± 0.02Functional Test: Meets or exceeds criteria for
high quality sequencing.Storage: -20°C
77205 25µmol(250µl) 100µmol(1ml) 500µmol(5ml)
2'-Deoxyinosine-5'-Triphosphate, Sodium Salt(dITP)
C10
H12
N4O
13P
3Na
3•2H
2O
Formula Weight: 594.14PRODUCT SPECIFICATIONSForm: PowderAssay (Total UV): 97 ± 4%Assay (HPLC): 97 ± 4%λ
max: 249 nm
εmax
: [11.8 ± 0.5] x 103
Spectral Ratios: A
250/A
260: 1.68 ± 0.04
A280
/A260
: 0.23 ± 0.02Reference Constants:λ
max (pH 6, DBR ): 269 nm
εmax
(pH 6, DBR ): 12.2 x 103
Storage: -20°CCAS #95648-77-4
14335 25mg
6-Deoxy-L-MannoseSee L-Rhamnose, page 282
2'-Deoxynucleoside-5'-Triphosphates, Sodium Salt, 100mM Solutions, Sets of Four(dNTPs)
ULTRAPUREForm: 100mM aqueous solutions, pH 7.5; dATP,
dCTP, dGTP, dTTPStorage: -20°C
4x25µmol(250µl)eachdNTPperpack77100 1pk4x100µmol(1ml)eachdNTPperpack77328 1pk4x500µmol(5ml)eachdNTPperpack77128 1pk
2'-Deoxynucleoside-5'-Triphosphate Mixes
See PCR Nucleotide Mixes, page 268
2-Deoxy-D-Ribose
ULTRAPUREC
5H
10O
4
Formula Weight: 134.13PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0%[α]20
D : -57.3° ± 1.5°(c=1 in H
2O)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.5%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmCAS #533-67-5
14420 5gm 25gm 100gm
2'-DeoxythymidineSee Thymidine, page 298
2'-Deoxythymidine-5'-Diphosphate, Sodium Salt(Thymidine-5'-Diphosphate)
C10
H13
N2O
11P
2Na
3•nH
2O
Formula Weight: 468.13•nH2O
Storage: -20°CCAS #108322-12-9
22310 10mg 25mg 100mg
2'-Deoxythymidine-5'-Monophosphate, Disodium Salt(Thymidine-5'-Monophosphate; Thymidylic Acid)
C10
H13
N2O
8PNa
2•nH
2O
Formula Weight: 366.17•nH2O
Reference Constants:λ
max (pH 7, NRC ): 267 nm
εmax
(pH 7, NRC ): 9.6 x 103
ε260
(pH 7, DBR ): 9.3 x 103
Storage: 4°CCAS #33430-62-5
22320 250mg 1gm
2'-Deoxythymidine-5'-Triphosphate, Trisodium Salt(dTTP; Thymidine-5'-Triphosphate)
C10
H14
N2O
14P
3Na
3•nH
2O
Formula Weight: 548.11•nH2O
PRODUCT SPECIFICATIONSForm: White powderAssay (pH 7.0): ≥ 96.0% (dry basis)Moisture: By KFλ
max (pH 7.0): 267 nm ± 1 nm
εmax
(pH 7.0): (9.6 ± 0.4) x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.65 ± 0.03
A280
/A260
: 0.73 ± 0.03Reference Constants:λ
max (pH 7, DBR ): 267 nm
εmax
(pH 7, DBR ): 9.6 x 103
ε260
(pH 7, DBR ): 9.3 x 103
Storage: -20°CCAS #18423-43-3
22325 10mg 25mg 50mg
Bioc
hem
ical
sBiochemicals
5� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
2'-Deoxythymidine-5'-Triphosphate, Lithium Salt, 100mM Solution(dTTP)
ULTRAPUREC
10H
13N
2O
14P
3Li
4
Formula Weight: 505.90PRODUCT SPECIFICATIONSForm: 100mM solution, pH 7.1Assay (HPLC): ≥ 98.0%pH: 7.0 ± 0.2λ
max: 267 nm ± 1 nm
Spectral Ratios: A
250/A
260: 0.64 ± 0.02
A280
/A260
: 0.74 ± 0.02Storage: -20°C
77308 25µmol(250µl)
2'-Deoxythymidine-5'-Triphosphate, Sodium Salt, 100mM Solution(dTTP)
ULTRAPUREC
10H
13N
2O
14P
3Na
4
Formula Weight: 570.10PRODUCT SPECIFICATIONSForm: 100mM aqueous solutionAssay (HPLC): ≥ 99%pH: 7.5 ± 0.2λ
max: 267 nm ± 1 nm
Spectral Ratios (pH 7.5): A
250/A
260: 0.65 ± 0.03
A280
/A260
: 0.73 ± 0.03Functional Tests: Meets or exceeds criteria for
high quality sequencing. Functionally tested in long PCR.
Storage: -20°C
77108 25µmol(250µl) 100µmol(1ml) 500µmol(5ml)
2'-DeoxyuridineC
9H
12N
2O
5
Formula Weight: 228.20Reference Constants:λ
max (pH 7, NRC ): 262 nm
εmax
(pH 7, NRC ): 10.2 x 103
ε260
(pH 7, DBR ): 10.1 x 103
CAS #951-78-0
14425 250mg 1gm 5gm 25gm
2'-Deoxyuridine-5'-Monophosphate, Disodium Salt(dUMP)
C9H
11N
2O
8PNa
2•nH
2O
Formula Weight: 352.15•nH2O
PRODUCT SPECIFICATIONS Form: White crystalline powderAssay (UV): 100 ± 5% (dry basis)Chromatography (TLC): Homogeneous Spectral Ratios:
A280
/A260
: 0.37 ± 0.03 A
250/A
260: 0.73 ± 0.03
Moisture (KF): ≤ 16%Reference Constants:λ
max (pH 7, NRC ): 262 nm
εmax
(pH 7, NRC ): 10.2 x 103
ε260
(pH 7, DBR ): 10.1 x 103
Storage: -20°CCAS #42155-08-8
14430 100mg 250mg 500mg 1gm
2'-Deoxyuridine-5'-Triphosphate, Sodium Salt, 100mM Solution(dUTP)
ULTRAPUREC
9H
11N
5O
12P
3Na
4
Formula Weight: 556.1Form: 100mM aqueous solutionpH: 7.5 ± 0.2Storage: -20°C
77206 25µmol(250µl) 100µmol(1ml) 500µmol(5ml)
DEPCSee Diethyl Pyrocarbonate, page 221
DexamethasoneUSBioAnalyzedC
22H
29FO
5
Formula Weight: 392.46PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (HPLC): 97.0 - 102.0% (dry basis)Identity: By IRChromatographic Purity: Passes testLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.2%O.V.I.: Meets requirementsCAS #50-02-2
76500 100mg 500mg 1gm 5gm
DextranM.W.: < 15,000CAS #9004-54-0
14475 10gm 100gm 1kg
DextranM.W.: 15,000 - 20,000CAS #9004-54-0
14485 100gm 1kg
DextranM.W.: 35,000 - 50,000CAS #9004-54-0
14490 100gm 1kg
Dextran
ULTRAPUREM.W.: 60,000 - 90,000PRODUCT SPECIFICATIONSForm: White powderpH (6% w/v in KCl): 5.0 - 6.5Loss on Drying: ≤ 7.0%Residue on Ignition: ≤ 1.9%CAS #9004-54-0
14495 10gm 100gm 1kg
Biochemicals
Biochemicals
55w w w . u s b w e b . c o m
DextranM.W.: 200,000 - 300,000PRODUCT SPECIFICATIONSForm: White powderLoss on Drying: ≤ 5.0%Ash: ≤ 2.0%pH (6%, H
2O): 5.0 - 7.0
CAS #9004-54-0
14500 100gm 1kg
DextranM.W.: 5,000,000 - 40,000,000CAS #9004-54-0
14505 100gm 1kg
Dextran Sulfate, Sodium SaltM.W.: < 15,000CAS #9011-18-1
14517 1gm 5gm 10gm 50gm
Dextran Sulfate, Sodium SaltM.W.: 40,000 - 50,000
14489 10gm 50gm 100gm
Dextran Sulfate, Sodium SaltM.W.: 500,000CAS #9011-18-1
14515 5gm 25gm 100gm
Dextran Sulfate, Sodium Salt
ULTRAPUREMW: 500,000*PRODUCT SPECIFICATIONSForm: White to off-white powderLoss on Drying: ≤ 10%pH (1%, H
2O): 5.0-7.5
Residue on Ignition: 40 - 50%*Note: Approximate molecular weight of dextran
used in the production of the sulfate. Average MW of dextran sulfate sodium salt is approx. 1,400,000.
CAS #9011-18-1
70796 10gm 50gm
DextrinCAS #9004-53-9
14530 5lb 25lb
D-(+)-Dextrose, Anhydrous(α-D-(+)-Glucose)
CH2OH(CHOH)
4CHO
Formula Weight: 180.16PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]25
D : +52.6° to +53.2° (dry basis, c=10 in
0.012N NH4OH)
Moisture (KF): ≤ 1.0%CAS #50-99-7
14535 1kg 5lb 25lb 100lb
D-(+)-Dextrose, Monohydrate(α-D-(+)-Glucose)
C6H
12O
6•H
2O
Formula Weight: 198.17
14536 10kg
Diadenosine Pentaphosphate, Trilithium Salt(Ap5A-Li)
C20
H26
N10
O22
P5Li
3•4H
2O
Formula Weight: 916.37 (as anhydrous free acid) 1006.23 (Ap5A•Li•4H
2O)
PRODUCT SPECIFICATIONSForm: White powderPurity (HPLC): ≥ 90%Lithium (Li): 3.0 ± 1.5%Moisture (KF): ≤ 10%Storage: -20°C, protect from moistureCAS #75522-97-3
26140 25mg 200mg
DL-α-ε-Diaminopimelic AcidC
7H
14N
2O
4
Formula Weight: 190.20CAS #583-93-7
14575 1gm 5gm 10gm
Diaphorase(E.C.1.8.1.4)Source: Clostridium kluyveriForm: Lyophilized powderUnit Definition: One unit causes a decrease in
absorbance of 1.0 per min due to the reduction of DCPIP by NADH 25°C, pH 7.5.
Storage: 4°C, protect from moisture.CAS #9001-18-7
14615 600units 1,800units
Diastase(E.C.3.2.1.2)Source: MaltForm: Powder stabilized with lactoseActivity: 500° Lintner units/gm
14625 100gm 500gm 1kg
Diastase(E.C.3.2.1.2)Source: MaltForm: Powder stabilized with lactoseActivity: 1,000° Lintner units/gm
14626 100gm 500gm
Bioc
hem
ical
sBiochemicals
56 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Diazo Blue B(Zinc Chloride o-Dianisidine, Tetrazotized)
C14
H12
Cl2N
4O
2•ZnCl
2
Formula Weight: 475.49CAS #20282-70-6
14645 100gm
2,4-Dichlorophenoxyacetic AcidC
8H
6Cl
2O
3
Formula Weight: 221.04PRODUCT SPECIFICATIONSForm: Cream to yellow colored flakesAssay: ≥ 97.0%Identity: By IRMoisture (KF): ≤ 1.0%Melting Point: 136 - 142°C CAS #94-75-7
14670 100gm 1kg
2',3'-DideoxyadenosineC
10H
13N
5O
2
Formula Weight: 235.24Storage: 4°CCAS #4097-22-7
14691 5mg 10mg 25mg
2',3'-Dideoxyadenosine-5'-Triphosphate(ddATP)
ULTRAPUREC
10H
12N
5O
11P
3Na
4
Formula Weight: 563.11PRODUCT SPECIFICATIONSAssay (FPLC): ≥ 99%pH: 7.5 ± 0.2λ
max: 259 nm ± 1 nm
Spectral Ratios (pH 7.5): A
250/A
260: 0.80 ± 0.03
A280
/A260
: 0.12 ± 0.02Functional Test: Meets or exceeds criteria for
high quality sequencing.Storage: -20°C
10mMaqueoussolution,pH7.577110 0.5µmol(50µl) 1µmol(100µl)100mMaqueoussolution,pH7.577331 4µmol(40µl) 50µmol(500µl)
2',3'-Dideoxycytidine-5'-Triphosphate(ddCTP)
ULTRAPUREC
9H
12N
3O
12P
3Na
4
Formula Weight: 539.08PRODUCT SPECIFICATIONSAssay (FPLC): ≥ 99%pH: 7.5 ± 0.2λ
max (pH 2.0): 280 nm ± 1 nm
Spectral Ratios (pH 2.0): A
250/A
260: 0.45 ± 0.03
A280
/A260
: 2.20 ± 0.20 A
290/A
260: 1.70 ± 0.20
Functional Test: Meets or exceeds criteria for high quality sequencing.
Storage: -20°C
10mMaqueoussolution,pH7.577112 0.5µmol(50µl) 1µmol(100µl)100mMaqueoussolution,pH7.577332 4µmol(40µl) 50µmol(500µl)
2',3'-Dideoxyguanosine-5'-Triphosphate(ddGTP)
ULTRAPUREC
10H
12N
5O
12P
3Na
4
Formula Weight: 579.11PRODUCT SPECIFICATIONSAssay (FPLC): ≥ 99%pH: 7.5 ± 0.2λ
max (pH 7.5): 252 nm ± 1 nm
Spectral Ratios (pH 7.5): A
250/A
260: 1.18 ± 0.04
A280
/A260
: 0.67 ± 0.03 A
290/A
260: 0.28 ± 0.03
Functional Test: Meets or exceeds criteria for high quality sequencing.
Storage: -20°C
10mMaqueoussolution,pH7.577114 0.5µmol(50µl) 1µmol(100µl)100mMaqueoussolution,pH7.577333 4µmol(40µl) 50µmol(500µl)
2',3'-Dideoxynucleoside-5'-Triphosphates, 10mM Solutions, Sets of Four(ddNTPs)
ULTRAPUREForm: 10mM aqueous solutions, pH 7.5; ddATP,
ddCTP, ddGTP, ddTTPStorage: -20°C
4x0.5µmol(50µl)77126 1pk4x1.0µmol(100µl)77125 1pk
2',3'-Dideoxynucleoside-5'-Triphosphates, 100mM Solutions, Set of Four(ddNTPs)
ULTRAPUREForm: 100mM aqueous solutions, pH 7.5; ddATP,
ddCTP, ddGTP, ddTTPStorage: -20°C
4x4µmol(40µl)eachddNTPperpack77335 1pk
2',3'-Dideoxythymidine-5'-Triphosphate(ddTTP)
ULTRAPUREC
10H
13N
2O
13P
3Na
4
Formula Weight: 554.10PRODUCT SPECIFICATIONSAssay (FPLC): ≥ 99%pH: 7.5 ± 0.2λ
max (pH 7.5): 267 nm ± 1 nm
Spectral Ratios (pH 7.5): A
250/A
260: 0.65 ± 0.03
A280
/A260
: 0.83 ± 0.03Functional Tests: Meets or exceeds criteria for
high quality sequencing.Storage: -20°C
10mMaqueoussolution,pH7.577116 0.5µmol(50µl) 1µmol(100µl)100mMaqueoussolution,pH7.577334 4µmol(40µl) 50µmol(500µl)
Biochemicals
Biochemicals
57w w w . u s b w e b . c o m
Diethyl Pyrocarbonate(DEPC)
ULTRAPUREO(COOC
2H
5)2
Formula Weight: 162.14PRODUCT SPECIFICATIONSForm: Clear, colorless liquidAssay: ≥ 97%Density (20°C): 1.121 ± 0.005Refractive Index (20°C): 1.398 ± 0.005Applications:•A strong, nonspecific inhibitor of ribonuclease•Reacts with the N1 of histidine residues in
proteins under mild conditions•Used to cross-link and modify proteins by
ethoxyformylation of NH2 and imidazole groups
in proteins•Reacts with amines and thus cannot be used
with Tris BufferReferences:1. Tsurushiin, S., Hiramatsu, A., Inamasu, M. and
Yasunobu, K. T. (1975) Biochim. Biophys. Acta. 400, 451-460.
2. Meyer, S. E. and Cromartie, T. H. (1980) Biochemistry 19, 1874-1881.
3. Berger, S. L. (1975) Anal. Biochem. 67, 428-437.
4. Leonard, N. J., Mcdonald, J. J., Henderson, R. E. and Reichmann, M. E. (1971) Biochemistry 10, 3335-3342.
5. Roberts, M. F., Deems, R. A., Mincey, T. C. and Dennis, E. A. (1977) J. Biol. Chem. 252, 2405-2411.
Storage: -20°CCAS #1609-47-8
14710 25ml 100ml
Dihydrostreptomycin SulfateUSBioAnalyzed(C
21H
41N
7O
12)2•3H
2SO
4
Formula Weight: 1461.42PRODUCT SPECIFICATIONSForm: White to off-white powderActivity: ≥ 650 µg of C
21H
41N
7O
12/mg (as is)
Identity (A, B): Passes testspH (200 mg dihydrostreptomycin/ml): 4.5 - 7.0Loss on Drying: ≤ 5.0%Streptomycin: ≤ 3.0%CAS #5490-27-7
14765 25gm 100gm
Dimedone(5,5-Dimethyl-1,3-Cyclohexanedione)
C8H
12O
2
Formula Weight: 140.18PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 99%Identity: By IRMelting Point: 147 - 150°CSolubility (5% EtOH): Passes testCAS #126-81-8
14850 10gm 100gm 1kg
p-Dimethylamino Benzaldehyde(CH
3)2NC
6H
4CHO
Formula Weight: 149.19PRODUCT SPECIFICATIONSForm: Light tan powderIdentity: By IRChromatography (TLC): HomogeneousMelting Point: 70 - 75°CCAS #100-10-7
14868 25gm 100gm 500gm
p-Dimethylamino Cinnamaldehyde(CH
3)2NC
6H
4CH:CHCHO
Formula Weight: 174.22CAS #6203-18-5
14872 25gm
N,N-Dimethyl Formamide
ULTRAPUREHCON(CH
3)2
Formula Weight: 73.10PRODUCT SPECIFICATIONSForm: Colorless, clear liquidAssay (GC): ≥ 99.5%Used for dissolving X-GAL and X-GLUC.CAS #68-12-2
14862 250ml
3,3-Dimethyl Glutaric AcidCH
2COOHC(CH
3)2CH
2COOH
Formula Weight: 160.17PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 98.0% (as is)Identity: By IRLoss on Drying: ≤ 2.0%CAS #4839-46-7
14910 10gm 25gm 100gm
5,5'-Dithiobis-(2-Nitrobenzoic) Acid(DTNB; Ellman’s Reagent)
C14
H6N
2O
8S
2
Formula Weight: 396.36PRODUCT SPECIFICATIONSForm: Light yellow crystalline powderAssay: ≥ 99.0% Chromatography (TLC): HomogeneousIdentity: By IR Melting Range: 236 - 248°CCAS #69-78-3
15375 5gm 25gm 100gm
Dithioerythritol(DTE)
ULTRAPUREC
4H
10O
2S
2
Formula Weight: 154.25Form: White crystalline powderChromatography (TLC): HomogeneousAssay (I
2): ≥ 99%
Melting Point: ≥ 81°CA (283 nm, 0.02M, H
2O): ≤ 0.04
Storage: -20°CCAS #6892-68-8
15385 1gm 5gm 25gm 100gm
Bioc
hem
ical
sBiochemicals
58 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Dithiothreitol(DTT; Cleland’s Reagent)
ULTRAPUREMB GradeC
4H
10O
2S
2
Formula Weight.: 154.25PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (SH): ≥ 99.5%A (280 nm, 0.1M, 1 cm): ≤ 0.08A (260 nm, 0.1M, 1 cm): ≤ 0.40Melting Point: 40 - 43°COxidized DTT: ≤ 0.3%DNase: Passes testRNase: Passes testProtease: Passes testApplication:•Used as a protective agent for SH groupsReference:1. Cleland,W. W. (1964) Biochemistry 3, 480-
482.Storage: -20°CCAS #3483-12-3
15397 1gm 5gm 25gm 100gm
Dithiothreitol(DTT; Cleland’s Reagent)
C4H
10O
2S
2
Formula Weight: 154.25PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97%Identity: By IRMelting Point: ≥ 38°COxidized DTT: ≤ 1%Storage: -20°CCAS #3483-12-3
15395 1gm 5gm 25gm 100gm
Dithiothreitol (DTT), 0.1M Solution
ULTRAPUREMB GradeForm: 0.1M solution of DTT in high purity dH
2O.
Solution is 0.2 µm filtered and dispensed into vials.
Storage: -20°C
70726 150µl 1ml 5ml
DNA, Bacteriophage Lambda
DNA, Calf Thymus
DNA, E. coli
DNA, Fish Sperm
DNA, Fish Sperm, Activated
DNA-Cellulose, Double-Stranded(dsDNA-Cellulose)
DNA-Cellulose, Single-Stranded(ssDNA-Cellulose)
See Molecular Biology Products & Kits Section, page 92
DNA Sequencing Gel Mix, TBE
ULTRAPUREMB GradeForm: Dry, pre-weighed, sequencing gel mixes
with matched TBE Buffer. The gel is an Ultrapure powdered mixture which, when dissolved in the appropriate volume of water, will result in a solution containing the specified acrylamide percentage plus bis-acrylamide (19:1 ratio), 7M urea and TBE Buffer.
Contents: Each bottle of gel mix, when reconstituted, yields 125 ml of gel solution. This volume is enough for 1 - 2 standard sequencing gels.
Testing: Functionally tested in sequencing gel electrophoresis.
To use: To prepare gel, add 77 ml high purity dH
2O to the 6% mix and dissolve completely at
65°C. Filter solution. Add 1.25 ml 10% (w/v) ammonium persulfate and 30 µl TEMED. Mix gently. Pour and allow 30 min to polymerize.
Note: One bottle of powdered Ultrapure TBE Buffer is included with each gel mix bottle. Each bottle reconstitutes to make one liter of 1X running buffer. Use with corresponding TBE Running Buffer, PN 70454.
72990 5bottles
DNA Solutions Kit
ULTRAPUREMB GradeForm: A selection of 8 Convenience Reagent
Solutions, 100 ml each. 0.5M EDTA, PN 15694 1M MgCl
2, PN 78641
5M NaCl, PN 75888 3M NaOAc, PN 75897 20% SDS, PN 75832 1X TE Buffer, PN 75893 1M Tris, pH 8.0, PN 22638 Water, Nuclease-Free, PN 71786
75910 1Kit
DNase ISource: Bovine pancreasForm: White crystalline powderActivity: >90,000 Dornase units/mg >2,500 Kunitz units/mgUnit Definition: One Kunitz unit causes an
increase in absorbance of 0.001 per min per ml at 260 nm at 25°C, pH 5, using the assay of Kunitz(1). One Kunitz unit = 35 Dornase units.
Reference:1. Kunitz (1950) J. Gen. Physiol. 33, 349-363.Storage: Store at 4°C.
14340 10mg 20mg 100mg
Dodecyl-β-D-Maltoside(Lauryl Maltoside)
ULTRAPUREC
24H
46O
11
Formula Weight: 510.62PRODUCT SPECIFICATIONSForm: White powderPurity (HPLC): ≥ 99%pH (1%, H
2O): 5 - 8
A (260 nm, 1%, H2O): ≤ 0.06
Application: •Non-ionic detergent used to solubilize
membrane proteinsStorage: -20°C
18223 500mg 1gm 5gm
Dodecyl Sulfate, Lithium SaltSee Lithium Dodecyl Sulfate, page 249
Biochemicals
Biochemicals
59w w w . u s b w e b . c o m
Dodecyl Sulfate, Sodium SaltSee Sodium Dodecyl Sulfate, page 286
N-Dodecyl Trimethylammonium Bromide(DTAB)
C15
H34
BrNFormula Weight: 308.34PRODUCT SPECIFICATIONSForm: White powderMelting Point: 239 - 247°C
18218 10gm 50gm 250gm
n-Dodecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate
See Sulfobetaine 12 (SB3-12), page 293
DPNSee Nicotinamide Adenine Dinucleotide, page 262
DTABSee N-Dodecyl Trimethylammonium Bromide,
page 223
DTESee Dithioerythritol, page 221
DTTSee Dithiothreitol, page 222
Dulcitol(Galactitol)
C6H
14O
6
Formula Weight: 182.17CAS #608-66-2
15440 25gm 100gm 500gm
EDTASee Ethylenediamine Tetraacetic Acid, page 225
Egg SolidWhole Egg, spray dried
15455 1lb 5lb 25lb
Egg WhiteSee Albumin-Egg, page 188
EGTASee Ethylene Glycol-o-o'-bis (2-aminoethyl)-
N,N,N',N',-Tetraacetic Acid, page 225
Elastase
ULTRAPURE(E.C.3.4.21.36)Source: Porcine PancreasPRODUCT SPECIFICATIONSForm: Aqueous suspension with 0.01% NaN
3
Activity: 60 to 80 units/mg proteinUnit Definition: One unit solubilizes 1 mg of
elastin/20 min at 37°C, pH 8.8.Storage: 4°C
15470 25mg 50mg 100mg
Elastase(E.C.3.4.21.11)Source: Porcine PancreasForm: Lyophilized, salt free powderUnit Definition: One unit solubilizes 1 mg of
elastin/20 min at 37°C, pH 8.8.Storage: 4°CCAS #9004-06-2
15475 5mg 10mg 20mg 100mg
Ellman’s ReagentSee 5,5'-Dithio-bis- (2-Nitrobenzoic) Acid,
page 221
Enolase(E.C.4.2.1.11)Source: YeastPRODUCT SPECIFICATIONSForm: 50% glycerol solutionSpecific Activity: ≥ 40 units/mg proteinContaminating Activities: Phosphoglyceromutase: ≤ 0.02% Pyruvate Kinase: ≤ 0.02%Glycerol: 50%K-PO
4: 0.1M ± 0.05M
pH: 7.0Unit Definition: One unit oxidizes 1 µmol of
NADH/min at 25°C, pH 7.5.Storage: -20°C
15515 10,000units
ErgocalciferolUSBioAnalyzedC
28H
44O
Formula Weight: 396.65PRODUCT SPECIFICATIONSForm: White to light yellow powderIdentification (A, B, C, D,): Passes testAssay: 97.0 - 103.0% (as is)[α]25
D : +103° to +106° (c= 1.5 in EtOH)
Melting Range: 115 - 119°CReducing Substances: Passes testResidual Solvents: Meets requirementsStorage: 4°C, protected from light sealed under
nitrogen
15562 2gm 5gm 25gm 100gm
ErgosterolC
28H
44O
Formula Weight: 396.65Storage: 4°C, in tightly closed containersCAS #57-87-4
15565 5gm 10gm 25gm 100gm
Bioc
hem
ical
sBiochemicals
60 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Erythromycin, Free BaseC
37H
67NO
13
Formula Weight: 733.93PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderActivity: ≥ 850 µg of C
37H
67NO
13/mg (dry basis)
≥ 765 µg of C37
H67
NO13
/mg (as is)Identity: By IR[α]25
D : -71° to -78° (c=2 in EtOH)
pH (40 mg/ml, 1:19/MeOH:H2O): 8.0 - 10.5
Moisture (KF): ≤ 10.0%Storage: Ambient in tight containersCAS #114-07-8
15881 5gm 25gm
EsculinC
15H
16O
9
Formula Weight: 340.28 (anhydrous)PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: 97.5 - 102.5%Identity: By IR[α]20
D : -84° to -87° (c=2 in Dioxane:H
2O/1:1)
Moisture (KF): 6.5 - 8.0%CAS #66778-17-4
15590 5gm 25gm 100gm
β-EstradiolUSBioAnalyzedC
18H
24O
2
Formula Weight: 272.38PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 97.0 - 103.0% (dry basis)Identity A: By IRIdentity B (by UV): Passes test[α]25
D : +76° to +83°, dry basis (c=1 in Dioxane)
Melting Point: 173 - 179°CMoisture (KF): ≤ 3.5%Chromatographic Purity: Passes testStorage: Hygroscopic. Protect from moistureCAS #50-28-2
15600 1gm 5gm
17- -Estradiol-3-BenzoateC
25H
28O
3
Formula Weight: 376.49PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98%Identity: By IRMelting Point: 190 - 201°C[α]25
D : +57° to 63° (c=1, Dioxane)
CAS #50-50-0
15611 250mg 1gm 5gm
EstriolC
18H
24O
3
Formula Weight: 288.38CAS #50-27-1
15615 100mg 500mg 1gm
Estrone(1,3,5-Estratriene-3-ol-17-one)
C18
H22
O2
Formula Weight: 270.37PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 97.0 - 103.0% (dry basis)Identity: By IR[α]20
D : +158° to +165° (c=1, Dioxane)
Loss on Drying: ≤ 5.0%Residue on Ignition: ≤ 0.5%CAS #53-16-7
15625 1gm 5gm 100gm
Ethanolamine, HydrochlorideH
2NCH
2CH
2OH•HCl
Formula Weight: 97.54PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRCAS #2002-24-6
15630 50gm 100gm
Ethidium Bromide
ULTRAPUREC
21H
20BrN
3
Formula Weight.: 394.31PRODUCT SPECIFICATIONSForm: Purple/maroon crystalline powderAssay: ≥ 98% (dry basis)Loss on Drying: ≤ 10%Residue on Ignition: ≤ 0.2%Ammonium Bromide: ≤ 0.1%Applications:•A fluorescent dye that binds to DNA by
intercalating between the bases, causing the double helix to unwind
•Used to detect both single-and double-stranded DNA and RNA
•Used in DNA isolation procedures and in gel electrophoresis
Stock Solution: 10 mg/ml 100 mg Ethidium Bromide 10 ml H
2O
Filter and store solution protected from light.Working Concentration: 0.5 µg/ml for gel
staining.Note: The electrophoretic mobility of linear
double-stranded DNA is reduced by 15% in the presence of ethidium bromide.
CAS #1239-45-8
32813 1gm 5gm 25gm
Ethidium Bromide Drops
ULTRAPUREForm: Ethidium bromide is supplied in a
convenient, ready-to-dispense dropper format for gel electrophoresis applications. One drop contains 40 µl or 25 µg of ethidium bromide.
Concentration: 0.625 mg/ml in water.Testing: Functionally tested for use in gel
electrophoresis.To Use: Simply add one drop for every 50 ml
of agarose and/or buffer for a working concentration of 0.5 µg/ml.
75816 5ml
DL-EthionineC
6H
13NO
2S
Formula Weight: 163.24CAS #67-21-0
15640 5gm 25gm
Biochemicals
Biochemicals
61w w w . u s b w e b . c o m
Ethylenediaminetetraacetic Acid, (EDTA), 0.5M Solution
ULTRAPUREMB GradeForm: 0.5M solution of EDTA•Na
2 in high purity
dH2O, pH 8.0. Solution is 0.2 µm filtered
and dispensed into durable, square Nalgene bottles.
pH: 8.0 ± 0.1Contaminant Testing:
DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
Application: •Chelating agent used in many enzyme buffers
and, at higher concentrations, as an enzyme inactivator
15694 100ml 500ml
Ethylenediaminetetraacetic Acid, (EDTA) Disodium Salt, Dihydrate
ULTRAPUREMB GradeC
10H
14N
2Na
2O
8•2H
2O
Formula Weight: 372.24PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0%A (260 nm, 0.1M, 1 cm): ≤ 0.2A (280 nm, 0.1M, 1 cm): ≤ 0.05Insoluble Matter: ≤ 0.005%pH (5%, H
2O): 4.0 - 6.0
Nitrotriacetic Acid: ≤ 0.1%Heavy Metals (Pb): < 0.005%Iron (Fe): ≤ 0.01%Arsenic (As): ≤ 1 ppmCalcium (Ca): Passes testSolubility (0.1M, H
2O): Completely clear and
colorlessContaminant Testing:
DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testApplication: •Chelating agent used in many enzyme buffers
and as an enzyme inactivator at high concentrations
Stock Solution (0.5M): 186.1 gm EDTA H
2O to 800 ml
Titrate with approximately 20 gm of NaOH pellets to pH 8.0. Bring solution to 1 liter with water and sterilize by autoclaving.
CAS #6381-92-6
15701 100gm 500gm 1kg
Ethylenediaminetetraacetic Acid, (EDTA) Disodium Salt, Dihydrate
ACS Reagent GradeC
10H
14N
2O
8Na
2•2H
2O
Formula Weight: 372.24PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0%pH (5%, H
2O, 25°C): 4.0 - 6.0
Insoluble Matter: ≤ 0.005%Nitrilotriacetic Acid: ≤ 0.1%Heavy Metals (Pb): ≤ 0.005%Iron (Fe): ≤ 0.01%A (260 nm, 0.1M, 1 cm): ≤ 0.2A (280 nm, 0.1M, 1 cm): ≤ 0.05Solubility: (0.1M, H
2O): Clear and colorless
CAS #6381-92-6
15699 100gm 500gm 1kg 5kg
Ethylenediaminetetraacetic Acid, (EDTA) Disodium Salt, Dihydrate
C10
H14
N2O
8Na
2•2H
2O
Formula Weight: 372.24PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0% (as disodium dihydrate)Identity: By IRSolubility (5%, H
2O): Clear, colorless to slightly
yellowpH (5%, H
2O): 4.0 - 6.0
pH (1%, H2O): 4.0 - 6.0
Note: This product is a general quality laboratory chelating reagent suitable for most educational purposes. 0.1M stock solutions of this grade may be pale yellow in color. For demanding research applications and for use with sensitive reagents, USB recommends Ultrapure Grade (PN 15701) or ACS Reagent Grade EDTA (PN 15699).
CAS #6381-92-6
15697 100gm 500gm 1kg
Ethylene Diamine Tetraacetic Acid, (EDTA) Tetrasodium Salt, Dihydrate
ULTRAPUREC
10H
12N
2O
8Na
4•2H
2O
Formula Weight: 416.20 (dihydrate)PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0% (as is)Identity: By IRpH (1%, H
2O): 10.7 - 11.7
pH (5%, H2O): 10.7 - 12.0
CAS #10378-23-1
15700 500gm 1kg
Ethylene Glycol-O,O'-Bis (2 Amino-ethyl)-N,N,N',N',-Tetraacetic Acid (EGTA)
ULTRAPUREC
14H
24N
2O
10
Formula Weight: 380.35PRODUCT SPECIFICATIONSForm: White to slightly off-white powderAssay: ≥ 99%Spectral Ratios (280 nm, 0.1M in 1N NaOH, 1
cm): ≤ 0.10Identity: By IRLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.005%Sulfate (SO
4): ≤ 0.02%
Cadmium (Cd): ≤ 5 ppmCobalt (Co): ≤ 5 ppmCopper (Cu): ≤ 5 ppmIron (Fe): ≤ 5 ppmLead (Pb): ≤ 5 ppmNickel (Ni): ≤ 5 ppmZinc (Zn): ≤ 5 ppmApplication: •Chelating agent useful in the determination of
calcium in the presence of magnesiumReference:1. Schmid, R. W. and Reilley, C. N. (1957) Anal.
Chem. 29, 264-268.CAS #67-42-5
15703 10gm 50gm 100gm
Bioc
hem
ical
sBiochemicals
6� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
N-EthylmaleimideC
6H
7NO
2
Formula Weight: 125.13Storage: 4°CCAS #128-53-0
15685 5gm 10gm 25gm 100gm
Fast Green FCF
ULTRAPURE(C.I.42053)C
37H
34N
2Na
2O
10S
3
Formula Weight: 808.85Applications:•Stain for collagen, muscle, cytoplasm and
connective tissue.(1) Also a protein stain useful in the quantitative evaluation of electrophoretic separations on polyacrylamide gels.(2,3)
•Particularly useful in isoelectric focusing since it does not bind to ampholytes.(4)
References:1. Lillie, R. D., (1940) Stain Technol., 15, 17-22.2. Wilson, C. M., (1979) Anal. Biochem., 96, 263-
278.3. Bartolini, M. B. et al., (1976) Anal. Biochem.,
71, 6-13.4. Allen, R. E., et al., (1980) Anal. Biochem., 104,
494-498.CAS #2353-45-9
32814 10gm 25gm 100gm
Ferric Ammonium Citrate, Brown(FCC Brown)
Description: A complex salt of undetermined structure, composed of iron, ammonia, and citric acid.
PRODUCT SPECIFICATIONSForm: Brown to reddish-brown crystalline powder
or brownish-yellow powderIdentification: Passes testSolubility (1%, H
2O): Passes test
15749 100gm 500gm
Ferric Ammonium Citrate, GreenCP GradeFormula: A complex salt of undetermined,
structure composed of iron, ammonia and citric acid.
PRODUCT SPECIFICATIONSForm: Green powderAssay: 14.5% - 16.0% FeIdentity (A, B, C): Passes testFerric Citrate: Passes testLead (Pb): ≤ 10 ppmMercury : ≤ 1 ppmOxalate: Passes testSulfate: ≤ 0.3%
15751 100gm 500gm
Ferric Chloride, HexahydrateACS Reagent GradeLump form, very hygroscopicFeCl
3•6H
2O
Formula Weight: 270.30PRODUCT SPECIFICATIONSForm: Brownish-yellow to orange lumpsAssay: 97.0 - 102.0%Insoluble Matter: ≤ 0.01%Nitrate (NO
3): ≤ 0.01%
Phosphorus Compounds (PO4): ≤ 0.01%
Sulfate (SO4): ≤ 0.01%
Copper (Cu): ≤ 0.003%Zinc (Zn): ≤ 0.003%Ferrous Iron (Fe++): ≤ 0.002%Substances not precipitated by ammonium
hydroxide (SO4): ≤ 0.1%
12192 500gm 2.5kg
Ferrous Sulfate, HeptahydrateACS Reagent GradeFeSO
4•7H
2O
Formula Weight: 278.01PRODUCT SPECIFICATIONSForm: Blue-green crystalline powderAssay: ≥ 99.0%Insoluble Matter: ≤ 0.01%Chloride (Cl): ≤ 0.001%Phosphate (PO
4): ≤ 0.001%
Copper (Cu): ≤ 0.005%Manganese (Mn): ≤ 0.05%Zinc (Zn): ≤ 0.005%Ferric Iron (Fe3+): ≤ 0.1%Substances not precipitated by ammonium
hydroxide (SO4): ≤ 0.05%
12193 500gm 2.5kg
Fetal Bovine Serum Cell Culture GradePRODUCT SPECIFICATIONSForm: Clear straw to amber liquidTotal Protein: 3.0 - 4.0 gm/dlHemoglobin: 20 mg/dlpH: 6.7 - 8.0Endotoxin: 10 EU/mlSterility: SterileMycoplasma: None detectedVirally tested and found negative for BVD, IBR,
and PI3.Storage: -20°C
15801 500ml
Fetal Bovine Serum (FBS)Cell Culture GradePRODUCT SPECIFICATIONSForm: Clear straw to amber liquidTotal Protein: 3.0 - 4.0 gm/dlHemoglobin: ≤ 20 mg/dlpH: 6.7 - 8.0Endotoxin: ≤ 10 EU/mlSterility: SterileMycoplasma: None detectedVirally tested and found negative for BVD, IBR,
and PI3. USB Fetal Bovine Serum is an excellent
supplement to culture media.Storage: -20°C
15762 500mI
FibrinogenSource: Bovine BloodPlasminogen FreeStorage: 4°CCAS #9001-32-5
15769
FibrinogenSource: Bovine Blood90% ClottableStorage: 4°CCAS #9001-32-5
15768 1gm 5gm
Biochemicals
Biochemicals
63w w w . u s b w e b . c o m
FibrinogenSource: Human Blood65% ClottablePRODUCT SPECIFICATIONSForm: Lyophilized powderProtein: ≥ 60%Clottable Protein: ≥ 60%Storage: -20°CCAS #9001-32-5
15774 1gm
Fish OilSource: MenhadenCAS #8002-50-4
34568 1qt 12qt
Flavin Adenine Dinucleotide, Disodium Salt, Hydrate(FAD)
C27
H31
N9O
15P
2Na
2
Formula Weight: 829.51 (anhydrous) 883.56 (trihydrate)PRODUCT SPECIFICATIONSForm: Orange-yellow crystalline powderAssay (UV, 450 nm, pH 7.0): ≥ 95.0% (dry basis,
disodium salt)Moisture (KF): ≤ 8.0%Note: This product contains a varying amount
of moisture ranging from a dihydrate to a tetrahydrate at the time of packaging.
Storage: 4°C, protect from light, dessicate.CAS #84366-81-4
15785 100mg 250mg 500mg 1gm
Flavin MononucleotideSee Riboflavin-5-Phosphate, page 283
FluorescamineC
17H
10O
4
Formula Weight: 278.26Storage: -20°CCAS #38183-12-9
15802 100mg
5-FluorouracilC
4H
3FN
2O
2
Formula Weight: 130.08PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (titrimetric): 98.5 - 101.0%Loss on Drying: ≤ 0.5%CAS #51-21-8
15875 250mg 1gm 5gm
Folic Acid(Pteroylglutamic Acid)
USBioAnalyzedC
19H
19N
7O
6
Formula Weight: 441.40PRODUCT SPECIFICATIONSForm: Yellow to yellow-orange crystalline powderAssay: 97.0 - 102.0% (dry basis)Chromatographic Purity: Passes testIdentity: Passes testMoisture (KF): ≤ 8.5%Residue on Ignition: ≤ 0.3%O.V.I.: Meets requirementsCAS #59-30-3
15880 10gm 25gm 100gm 1kg
Formamide
ULTRAPURELow ConductivityHCONH
2
Formula Weight: 45.04Form: Clear, colorless liquidPRODUCT SPECIFICATIONSAssay: ≥ 99%Refractive Index (20°C): 1.447 ± 0.001Density (20°C): 1.133 ± 0.001A (280 nm, 100%, 1 cm): ≤ 0.15A (260 nm, 100%, 1 cm): ≤ 2.3pH (50% v/v with H
2O): 6.0 - 8.0
Freezing Point: 2.0 - 3.0°CConductivity: ≤ 100 µmhos/cmCopper (Cu): ≤ 0.2 ppmIron (Fe): ≤ 1 ppmLead (Pb): ≤ 1 ppmZinc (Zn): ≤ 1 ppmStorage: 4°CApplications:•For use in nucleic acid hybridization buffers,
specialized sequencing gels and a variety of other molecular biology applications to reduce the T
m of nucleic acid hybrids
•Used to achieve stronger denaturing conditions in a sequencing gel along with 7-deaza-dGTP to resolve sequencing gel compressions
To prepare a formamide sequencing gel: Place 40 ml Formamide in a beaker with
magnetic stir bar. Acrylamide 7.6 gm N-N'-Methylene-bis-Acrylamide 0.4 gm Urea 42.0 gm 10X TBE Buffer 10 ml1. Cover with parafilm and warm in a 65°C water
bath with stirring until dissolved. This takes 30-60 minutes and the mixture reaches a temperature of about 50°C.
2. When completely dissolved, add water to a total volume of 100 ml.
3. Cover and continue stirring at room temperature until it reaches < 30°C.
4. Vacuum filter with a nitrocellulose filter.5. Add 1 ml of 10% ammonium persulfate and
0.15 ml of TEMED. Pour immediately. (Pouring this viscous mixture requires nearly a vertical angle for the glass plates.) The gel should be polymerized within 30 minutes.
Formamide gels should be run at the same power (wattage) as regular gels. This requires a higher voltage (60% higher) than normal gels, and DNA migrates about half as fast.
Soaking the gel in 5% acetic acid, 20% methanol helps prevent swelling. Soak 15 min for a 0.4 mm thick gel and dry normally.
Compression artifacts are caused whenever stable secondary structures exist in the DNA under the conditions prevailing in the gel matrix during electrophoresis.
The folded structure runs faster through the gel matrix than equivalent unfolded DNA, catching up with the smaller DNA in the sequence. Currently, gel compression artifacts are eliminated in one of two ways. One is to change to a stronger denaturing condition, adding formamide as shown here, and the other is to use an analog of dGTP (dITP or 7-deaza-dGTP) during synthesis.
Formamide Hybridization Buffer: 50% Formamide, 5X SSC, 0.5% SDS, 5X
Denhardt’s Solution, 100 µg/ml sonicated fish sperm DNA
Reagent Amount for every 100 ml Formamide* 50 ml 20X SSC* 25 ml 20% SDS* 2.5 ml 50X Denhardt’s Solution* 10 ml 10 mg/ml fish DNA 1.0 ml Water* up to 100 ml*Denotes Ultrapure Reagent available from USB.References:1. Sarkar, G., Kapelner, S. and Sommer, S. S.
(1990) Nucl. Acids Res. 18, 7465.2. Sambrook, J., Fritsch, E. F. and Maniatis, T.
(1989) Molecular Cloning, 2nd Edition, Cold Spring Harbor Laboratory.
CAS #75-12-7
75828 500gm 1kg
Bioc
hem
ical
sBiochemicals
6� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
-D-Fructose(β-D-Levulose)
C6H
12O
6
Formula Weight: 180.16PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 100 ± 2% (dry basis)Identity: By IRLoss on Drying: ≤ 0.5%Glucose: ≤ 0.5%Hydroxymethylfurfural: ≤ 0.1%Residue on Ignition: ≤ 0.5%Chloride (Cl): ≤ 0.018%Sulfate (SO
4): ≤ 0.025%
Arsenic (As): ≤ 1 ppmHeavy Metals (Pb): ≤ 5 ppmCAS #57-48-7
18425 500gm 1kg 10kg
D-Fructose-6-Phosphate, Disodium Salt
C6H
11Na
2O
9P
Formula Weight: 304.10 (anhydrous) 322.11 (monohydrate)Practically free of Fructose-1,6-DiphosphateStorage: -20°CCAS #26177-86-6
15929 500mg 1gm 5gm
α-L-(–)-Fucose(6-Deoxy-L-Galactose)
C6H
12O
5
Formula Weight: 164.16CAS #2438-80-4
15960 1gm 5gm 25gm 100gm
D-(+)-Fucose(6-Deoxy-D-Galactose)
C6H
12O
5
Formula Weight: 164.16CAS #3615-37-0
15955 1gm 5gm 25gm
Fumaric Acid, Free AcidHOOC(CH)
2COOH
Formula Weight: 116.07CAS #110-17-8
15970 1kg
G-418 Sulfate(Geneticin®, Antibiotic G-418)
ULTRAPUREC
20H
40N
4O
10•2H
2SO
4
Formula Weight: 692.71Storage: 4°C
11379 100mg 500mg 1gm 5gm
α-D-Galactose-1-Phosphate, Dipotassium Salt, Pentahydrate
C6H
11O
9PK
2•5H
2O
Formula Weight: 426.39 (pentahydrate)PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 99.0%Identity: By IRMoisture (KF): ≤ 23.0%[α]20
D : +81.5° ± 1.5° (c=2, H
2O, as
pentahydrate)[α]20
D : +103.3° ± 4.5° (c=2, H
2O, dry basis)
Storage: -20°C
16014 250mg 1gm 5gm
D-(+)-GalactoseC
6H
12O
6
Formula Weight: 180.16PRODUCT SPECIFICATIONSForm: White to off-white powderIdentity: By IR[α]20
D : ≥ +77° (c=4 in H
2O and NH
3)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.5%Glucose: ≤ 2.0%CAS #59-23-4
15995 100gm 500gm 1kg 5kg
D-(+)-GalactoseHigh Purity GradeC
6H
12O
6
Formula Weight: 180.16PRODUCT SPECIFICATIONSForm: White powderAssay (HPLC): ≥ 98% (by HPLC)Identity: By IR[α]20
D : +80.0° ± 0.5° (c=5 in H
2O and NH
3)
Solubility: Clear and colorlessLoss on Drying: ≤ 0.1%Residue on Ignition: ≤ 0.05%Arsenic (As): ≤ 0.5 ppmIron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 10 ppmGlucose: ≤ 0.1%CAS #59-23-4
15996 100gm 500gm 1kg 5kg
GDPSee Guanosine-5'-Diphosphate, page 237
Gel Electrophoresis ReagentsEthidium Bromide Drops, page 224Glycerol Tolerant Gel (GTG) Buffer, 20X Solution,
page 234RapidRun Agarose Buffer, 20X Solution, page 282TAE Buffer, 10X Solution, page 293TAE Buffer, 50X Solution, page 293TBE Buffer, 5X Solution, page 294TBE Buffer, 10X Ready-Mixed Powder, page 294Tris-Glycine (TG) Buffer, 10X Solution, page 301Tris-Glycine-SDS (TGS) Buffer, 10X Solution,
page 301
Gel Mixes and SolutionsDNA Sequencing Gel Mix, TBE Buffer, 6%,
page 222Glycerol Tolerant Gel (GTG) Buffer, 20X Solution,
page 234Glycerol Tolerant Gel (GTG) Buffer, 20X Ready-
Mixed Powder, page 234RapidGel™ 40% Liquid Acrylamide Stock
Solution, page 281RapidGel DNA Sequencing Gel Solution, TBE
Buffer, 4%, page 281RapidGel DNA Sequencing Gel Solution, TBE
Buffer, 6%, page 281RapidGel DNA Sequencing Gel Solution, TBE
Buffer, 8%, page 281RapidGel-XL 6% DNA Sequencing Gel Solution,
page 282RapidGel-XL 40% Liquid Acrylamide Stock
Solution, page 282Stop Solution, page 291
Biochemicals
Biochemicals
65w w w . u s b w e b . c o m
GelatinSource: Porcine SkinBloom 175We recommend this grade for most applications.CAS #9000-70-8
16045 1lb 5lb 25lb 100lb
GelatinSource: Porcine SkinBloom 300CAS #9000-70-8
16047 1lb 5lb 25lb 100lb
Gelatin
ULTRAPURESource: Porcine SkinPRODUCT SPECIFICATIONSAppearance: Pale yellow powderpH (1%, H
2O, slurry): 4.0 - 6.0
Moisture (KF): ≤ 12%Residue on Ignition: ≤ 2%Viscosity (6.66% at 60°C): 30.0 mpsMesh Size: 30 - 50Microbiological Testing: Aerobic Plate Count/gram: ≤ 200 cfu/gm Coliform/gram: ≤ 10 Yeast and Mold/gram: ≤ 10 S. aureus : Negative P. aeuruginosa : Negative Salmonella/10 grams: NegativeFunctionally tested in Polymerase Chain ReactionApplications:•Enzyme stabilizer•Used in buffers for dilution and storage of
bacteriophage stocks (SM buffer)CAS #9000-70-8
70086 100gm
Gelatin HydrolysateSource: EnzymaticPRODUCT SPECIFICATIONSForm: Tan to yellowish powderpH: 7.0 ± 0.2Moisture: Approx. 5%Residue on Ignition: ≤ 5%Microbiological Testing: Aerobic Plate Count cfu/gm: ≤ 5000 Coliform cfu/gm: ≤ 10 Yeast & Mold cfu/gm: ≤ 100
16024 1lb 5lb 25lb
Geneticin® DisulfateSee G-418 Sulfate, page 228
Gentamicin SulfateUSBioAnalyzedPRODUCT SPECIFICATIONSForm: White to buff-colored powderActivity: ≥ 590 µg of gentamicin/mg (dry basis) ≥ 484 µg of gentamicin/mg (as is)Identity (A, B): Passes testspH (4%, H
2O): 3.5 - 5.5
[α]25D : +107° to +121° (c=1 in H
2O)
Loss on Drying: ≤ 18.0%Residue on Ignition: ≤ 1.0%Gentamycin’s content: C
1: 25 - 50%
C2 + C
2a: 25 - 55%
C1a
: 10 - 35%Limit of Methanol: ≤ 1.0%Mode of Action:•Bactericidal, inhibits normal protein synthesis.Applications:•Very stable•Autoclavable•Requires no refrigeration•Effective when combined with an antibiotic that
interferes with cell wall synthesis.CAS #1405-41-0
16051 250mg 1gm 5gm 25gm
Gentian VioletSee Methyl Violet, page 258
β-GentiobioseC
12H
22O
11
Formula Weight: 342.30CAS #554-91-6
16055 250mg 500mg 1gm
Gibberellic Acid, Potassium SaltContent: 75% (balance is monopotassium
phosphate)
16068 1gm 5gm
Gibberellic Acid, Potassium SaltContent: 10% (balance is monopotassium
phosphate)
16065 1gm 5gm 10gm
Gibberellin A3
C19
H22
O6
Formula Weight: 346.37PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 900 mcg/mgLoss on Drying: ≤ 3%CAS #77-06-5
16069 1gm 10gm
γ-Globulins, Fraction IISource: Bovine BloodStorage: 4°CCAS #68476-36-8
16021 1gm 5gm 10gm 25gm
Bioc
hem
ical
sBiochemicals
66 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
-GlobulinsSource: Rabbit BloodDescription: Rabbit gamma globulin is purified
from rabbit plasma or serum.Purity: ≥ 95%Immunoelectrophoresis (IEP): Passes testStorage: 4°CCAS #68476-36-8
16103 500mg 1gm
GlucanSource: Barley GumThis material contains β-1,3 and β-1,
4-glucosidic linkages and is an important substrate for enzyme assays.
CAS #9041-22-9
16102 50mg 100mg 500mg 1gm 5gm
D-(+)-Glucosamine, HydrochlorideC
6H
13NO
5•HCl
Formula Weight: 215.63PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : +72.5° ± 1.5° (c=4, H
2O, 3 hr)
Loss on Drying: ≤ 0.5%CAS #66-84-2
16110 100gm 500gm 1kg
α-D-(+)-GlucoseSee Dextrose, page 219
Glucose Dehydrogenase(E.C.1.1.1.47)Source: Bacillus megateriumForm: Lyophilized powderUnit Definition: One unit oxidizes 1 µmol of
D-Glucose/min at 37°C.Storage: 4°CCAS #9028-53-9
16144 500units 1,000units 5,000units 10,000units
Glucose Oxidase(E.C.1.1.3.4)Source: Aspergillus nigerForm: 0.1M Sodium Acetate solutionActivity: Approx. 260 units/mg proteinProtein Concentration: Approx. 20 mg protein/mlContaminating Activities: Amylase: ≤ 0.05% Saccharase: ≤ 0.05% Maltase: ≤ 0.05%Unit Definition: One unit oxidizes 1 µmol of
glucose/min at 25°C, pH 7.0.Storage: 4°C
16146 50,000units 250,000units 1mu
β-D-Glucose PentaacetateC
16H
22O
11
Formula Weight: 390.34PRODUCT SPECIFICATIONSForm: White powder[α]25
D : +4.0° ± 1.0° (c=2.5 to 4.0 in Chloroform)
Melting Point: 130 - 133°CLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals (as Pb): ≤ 10 ppmIron: ≤ 5 ppmArsenic: ≤ 0.5 ppm
16150 100gm 500gm 1kg
α-D-Glucose-1-Phosphate, Disodium Salt, Tetrahydrate
C6H
11Na
2O
9P•4H
2O
Formula Weight: 376.16PRODUCT SPECIFICATIONSForm: White powderAssay (enzymatic): ≥ 95.0%Identity: By IRMoisture (KF): ≤ 20.0%CAS #56401-20-8
16165 5gm 25gm
D-Glucose-6-Phosphate, Barium Salt, Heptahydrate
C6H
11BaO
9P•7H
2O
Formula Weight: 521.56Storage: -20°CCAS #58823-95-3
16170 5gm 10gm
D-Glucose-6-Phosphate, Dipotassium Salt
Formula: C6 H
11O
9 PK
2
Formula Weight: 336.32Storage: -20°CCAS #5996-17-8
16180 1gm 5gm 10gm 25gm
D-Glucose-6-Phosphate, Disodium Salt, Dihydrate
C6H
11Na
2O
9P•2H
2O
Formula Weight: 340.13 (dihydrate)PRODUCT SPECIFICATIONSForm: White powderAssay (enzymatic): ≥ 95%Storage: -20°C, protect from moisture.CAS #3671-99-6
16185 1gm 5gm 10gm
Glucose-6-Phosphate Dehydrogenase(E.C.1.1.1.49)Source: Leuconostoc mesenteroidesPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 400 NAD units/mg protein ≥ 80 NAD units/mg powder ≥ 222 NADP units/mg protein Protein: ≥ 10%Na-Citrate: ≥ 10%KPO
4 ≥ 5%
Contaminating Activities: Hexokinase: ≤ 0.01% Phosophoglucose Isomerase: ≤ 0.005% Phosphogluconate Dehydrogenase: ≤ 0.001% Creatine Kinase: ≤ 0.001% Glutathione Reductase: ≤ 0.001% Phosphoglucomutase: ≤ 0.001% Myokinase: ≤ 0.001% Lactate Dehydrogenase: ≤ 0.01%Note: The above product is available in
manufacturing quantities as a lyophilized powder or an ammonium sulfate suspension. Please inquire for bulk pricing.
Storage: -20°C, protect from moistureCAS #9001-40-5
16193 500units 5,000units 10,000units
Biochemicals
Biochemicals
67w w w . u s b w e b . c o m
Glucose-6-Phosphate Dehydrogenase(E.C.1.1.1.49)Source: Leuconostoc mesenteroidesPRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionActivity: ≥ 700 units/mg protein (NAD units)Activity: ≥ 220 units/mg protein (NADP units)Protein Concentration: ≥ 8 mg/mlUnit Definition: One NAD unit reduces 1 µmol
NAD/min at 30°C, pH 7.8.Contaminating Activities: Hexokinase: ≤ 0.01% Phosphoglucose Isomerase: ≤ 0.005% Phosphogluconate Dehydrogenase: ≤ 0.001% Creatine Kinase: ≤ 0.001% Glutathione Reductase: ≤ 0.001% Phosphoglucomutase: ≤ 0.001% Myokinase: ≤ 0.001% Lactate Dehydrogenase: ≤ 0.01%Note: The above product is available in
manufacturing quantities as a lyophilized powder or an ammonium sulfate suspension. Please inquire for bulk pricing.
Storage: 4°C
16171 1,000units 5,000units 10,000units
Glucose-6-Phosphate Dehydrogenase(E.C.1.1.1.49)Source: YeastPRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionActivity: ≥ 250 units/mg proteinUnit Definition: One unit reduces 1 µmol NADP/
min at 25°C, pH 7.8.Contaminating Activities: Glutathione Reductase: ≤ 0.2% Hexokinase: ≤ 0.02% Phosphogluconate Dehydrogenase: ≤ 0.01% Glucosephosphate Isomerase: ≤ 0.01% Phosphoglucomutase: ≤ 0.01% Creatine Kinase: ≤ 0.001% Myokinase: ≤ 0.01% ATPase: ≤ 0.001%Note: The above product is available in
manufacturing quantities as a lyophilized powder or an ammonium sulfate suspension. Please inquire for bulk pricing.
Storage: 4°C
16176 500units 1,000units 5,000units 10,000units
Glucose-6-Phosphate Dehydrogenase(E.C.1.1.1.49)Source: YeastPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 250 units/mg proteinUnit Definition: One unit reduces 1 µmol NADP/
min at 25°C, pH 7.8.Contaminating Activities: Glutathione Reductase: ≤ 0.2% Hexokinase: ≤ 0.02% Phosphogluconate Dehydrogenase: ≤ 0.01% Glucosephosphate Isomerase: ≤ 0.01% Phosphoglucomutase: ≤ 0.01% Creatine Kinase: ≤ 0.001% Myokinase: ≤ 0.01% ATPase: ≤ 0.001%Note: The above product is available in
manufacturing quantities as a lyophilized powder or an ammonium sulfate suspension. Please inquire for bulk pricing.
Storage: -20°CCAS #9001-40-5
16177 500units 1,000units 5,000units 10,000units
α-D-Glucosidase(Maltase)
(E.C.3.2.1.20)Source: S. cerevisiaePRODUCT SPECIFICATIONSForm: Lyophilized, ammonium sulfate free powderSpecific Activity: ≥ 50 units/mg proteinProtein: 20-80%KPO
4: 5-35%
BSA: 10-50%Contaminating Activities: α-Galactosidase: ≤ 0.01% β-Galactosidase: ≤ 0.01% β-Glucosidase: ≤ 0.01%Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°CCAS #9001-42-7
16188 500units 5,000units
Glucuronolactone(D-Glucuronic Acid, Lactone)
C6H
8O
6
Formula Weight: 176.12CAS #32499-92-6
16195 50gm 100gm 1kg
Glutamate Dehydrogenase(E.C.1.4.1.3)Source: Bovine LiverPRODUCT SPECIFICATIONSForm: Lyophilized, chromatographically purifiedUnit Definition: One unit catalyzes the oxidation
of 1 µmol of NADH/min at 37°C under assay conditions not containing ADP.
Storage: -20°C
16250 100mg 500mg 1gm
Glutamate DehydrogenaseNADP Specific(E.C.1.4.1.4)Source: YeastPRODUCT SPECIFICATIONSForm: Lyophilized ammonium sulfate free powderActivity: ≥ 10 units/mgUnit Definition: One unit catalyzes the
transformation of 1 µmol of L-glutamate/min at 25°C, pH 9.0.
Note: The above product is available in manufacturing quantities as a lyophilized powder or a glycerol solution. Please inquire for bulk pricing.
Storage: -20°CCAS #9001-46-1
16252 5,000units 10,000units 50,000units
D-Glutamic Acid(D-α-Aminoglutaric Acid)
HOOCCH2CH
2CH(NH
2)COOH
Formula Weight: 147.13CAS #6893-26-1
16205 5gm 25gm 100gm
Bioc
hem
ical
sBiochemicals
68 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
DL-Glutamic Acid, Monohydrate(DL-α-Aminoglutaric Acid)
NH2CH(COOH)CH
2CH
2COOH•H
2O
Formula Weight: 165.14 (monohydrate)PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (monohydrate)Identity: By IRMoisture (KF): 10-12%Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.2%Heavy Metals (Pb): ≤ 10 ppmArsenic (As
2O
3): ≤ 2 ppm
Chloride (Cl): ≤ 0.07%CAS #19285-83-7
16210 25gm 100gm 1kg
L-Glutamic Acid(L-α-Aminoglutaric Acid)
HOOCCH2CH
2CH(NH
2)COOH
Formula Weight: 147.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IR[α]20
D : +31.2° to +32.2° (c=10 in 2N HCl)
Loss on Drying: ≤ 0.1%Heavy Metals (Pb): ≤ 20 ppmArsenic (As): ≤ 1.5 ppmLead (Pb): ≤ 10 ppmCAS #56-86-0
16215 500gm 1kg 5kg 25kg
L-Glutamic Acid, HydrochlorideNH
2CH(COOH)CH
2CH
2COOH•HCl
Formula Weight: 183.59PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +25.2° to +25.8° (c=10 in 2N HCl)
Loss on Drying: ≤ 0.5%CAS #138-15-8
16220 500gm 1kg 5kg
L-Glutamic Acid, Monosodium Salt, Monohydrate(Monosodium Glutamate)
USBioAnalyzedC
5H
8NNaO
4•H
2O
Formula Weight: 187.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5%Identity: By IRIdentification (A, B, C,): Passes testsClarity and color of solution: Passes testpH (5%, H
2O): 6.7 - 7.2
[α]20D : +24.8° to +25.3° (c=10 in 2N HCl)
Loss on Drying (100°C, 5 hrs): ≤ 0.5%Heavy Metals (Pb): ≤ 20 ppmChloride (Cl): ≤ 0.25%Lead (Pb): ≤ 10 ppmResidual Solvents: Meets requirementsCAS #6106-04-3
16245 1kg 5kg
L-Glutamic Acid, Potassium SaltKOOCCH
2CH
2CH(NH
2)COOH•H
2O
Formula Weight: 203.23PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRpH (2% in H
2O): 6.7 - 7.3
[α]20D : +22.2° to +24.0° (c=10 in 2N HCl)
Loss on Drying: ≤ 0.1%Heavy Metals (Pb): ≤ 20 ppmArsenic (As): ≤ 3 ppmChloride (Cl): ≤ 0.1%CAS #6382-01-0
16240 100gm 500gm 1kg
D-GlutamineH
2NCOCH
2CH
2CH(NH
2)COOH
Formula Weight: 146.14CAS #5959-95-5
16270 1gm 5gm 10gm
L-Glutamine(L-α-Aminoglutamic Acid)
H2NCOCH
2CH
2CH(NH
2)COOH
Formula Weight: 146.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IR[α]20
D : +6.3° to +7.3° (c=4 in H
2O)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmArsenic (As): ≤ 3 ppmChloride (Cl): ≤ 0.021%Iron (Fe): ≤ 10 ppmSulfate (SO
4): ≤ 0.028%
CAS #56-85-9
16285 25gm 100gm 250gm 500gm 1kg
Glutamyl Cysteinyl GlycineSee Glutathione, page 232
γ-L-Glutamyl-p-Nitroanilide, Free BaseC
11H
13N
3O
5•H
2O
Formula Weight: 285.25Storage: 4°CCAS #7300-59-6
16254 250mg 500mg 1gm 5gm 25gm
Glutathione-OxidizedC
20H
32O
12N
6S
2
Formula Weight: 612.63PRODUCT SPECIFICATIONSForm: White lyophilized powderAssay: ≥ 98%Identity: By IR[α]25
D : -98° to -106° (c=2 in H
2O)
Moisture (KF): ≤ 5%Heavy Metals (Pb): ≤ 10 ppmIron (Fe): ≤ 10 ppmStorage: 4°CCAS #27025-41-8
16320 250mg 500mg 1gm
Biochemicals
Biochemicals
69w w w . u s b w e b . c o m
Glutathione-Reduced(γ-L-Glutamyl-Cysteinyl Glycine)
C10
H17
O6N
3S
Formula Weight: 307.32PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98%Identity: By IR[α]20
D : -15° to -18° (c=2 in H
2O)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.2%Heavy Metals (Pb): ≤ 20 ppmArsenic (As): ≤ 2 ppmIron (Fe): ≤ 5 ppmCAS #70-18-8
16315 5gm 25gm 100gm
Glutathione Reductase(E.C.1.8.1.7)Source: YeastPRODUCT SPECIFICATIONSForm: 50% Glycerol solutionNote: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°C
16311 100units 1,000units
GlutenSource: WheatCAS #8002-80-0
16300 5lb 25lb 100lb
DL-Glyceric Acid, Calcium Salt[HOCH
2CH(OH)COO]
2Ca•2H
2O
Formula Weight: 286.25CAS #67525-74-0
16345 5gm 10gm
GlycerinSee Glycerol, page 233
GlycerolACS Reagent GradeC
3H
5(OH)
3
Formula Weight: 92.09PRODUCT SPECIFICATIONSForm: Clear, colorless, viscous liquidAssay (by vol.): ≥ 99.5%Color (APHA): ≤ 10Residue on Ignition: ≤ 0.005%Neutrality: Passes testChlorinated Compounds (as CI): ≤ 0.003%Sulfate (SO
4): ≤ 0.001%
Acrolein and Glucose: Passes testFatty Acid Esters: ≤ 0.05%Substances Darkened by Sulfuric Acid: Passes
testHeavy Metals (Pb): ≤ 2 ppmWater (H
2O): ≤ 0.5%
16371 500ml 1L
Glycerol(Glycerin)
USBioAnalyzedC
3H
8O
3
Formula Weight: 92.09PRODUCT SPECIFICATIONSForm: Colorless liquidAssay: 99.0 - 101.0%Color: Passes testIdentity (A, B): Passes testsSpecific Gravity: ≥ 1.249Moisture (KF): ≤ 5.0%Residue on Ignition: ≤ 0.01%Chlorinated Compounds: ≤ 0.003%Fatty Acids & Esters: Passes testSulfate (SO
4): ≤ 0.002%
Heavy Metals (Pb): ≤ 5 ppmChloride (Cl): ≤ 0.001%Residual Solvents: Meets the requirementsLimit of Diethylene Glycol and Related
Compounds: Meets the requirementsCAS #56-81-5
16375 500gm 1kg 4kg
Glycerol
ULTRAPUREMB GradeHOCH
2CH(OH)CH
2OH
Formula Weight: 92.09PRODUCT SPECIFICATIONSForm: Clear, colorless, viscous liquidAssay: ≥ 99.5%Identity: By IRSpecific Gravity (25°C): ≥1.249A (260 nm, 100%, 1 cm): ≤ 0.06A (280 nm, 100%, 1 cm): ≤ 0.05Color (APHA): ≤ 10Neutrality: Passes testResidue on Ignition: ≤ 0.005%Moisture: ≤ 0.5%Acrolein, Glucose and Ammonium Compounds:
Passes testFatty Acid Esters: ≤ 0.2 meq/100 gmSilver Reducing Substances: Passes testReadily Carbonizable Substances: Passes testRefractive Index: 1.470-1.475Chlorinated Compounds: ≤ 0.003%Chloride (Cl): ≤ 0.001%Sulfate (SO
4): ≤ 0.002%
Arsenic (As): ≤ 1.5 ppmCalcium (Ca): ≤ 5 ppmIron (Fe): ≤ 5 ppmMagnesium (Mg): ≤ 1 ppmMercury (Hg): ≤ 10 ppmZinc (Zn): ≤ 2 ppmHeavy Metals (Pb): ≤ 2 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testProtease: Passes testApplications:•Used for long-term storage of bacterial cultures•Step gradients for bacteriophage/particle
purification•Mammalian cell transfection•Storage buffers for enzymesTypical storage for bacterial cultures: Saturated bacterial culture 0.85 ml Glycerol, Ultrapure, sterile 0.15 ml Vortex the solution and transfer to a screw cap
storage tube. Freeze quickly and store at -70°C.CAS #56-81-5
16374 500ml 1L
Bioc
hem
ical
sBiochemicals
70 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Glycerol Tolerant Gel (GTG) Buffer, 20X Ready-Mixed Powder
ULTRAPUREMB GradeGlycerol Tolerant Gel (GTG) Buffer is formulated to resolve the problem of gel distortion associated with sequencing samples that contain glycerol. Standard TBE buffer causes gel distortion between 300-500 bases as a result of reactions between glycerol and boric acid to form an anionic ester compound, which migrates in the sequencing gel. This buffer also works equally well with samples that contain no glycerol.Form: Dry, white crystalline powder. After
reconstitution in 250 ml of high purity dH2O,
20X bottle contains 1.78M Tris, 0.57M Taurine and 0.01M EDTA. Dilute to 1X with high purity dH
2O for a solution consisting of 0.089M
Tris, 0.029M Taurine and 0.5mM EDTA. Total volume is 1.5 liters of 20X buffer or 30 liters of 1X buffer.
pH (1:20, 25°C): 8.9 ± 0.1Conductivity (1:20): 830 ± 100 µmhos/cmTesting: Meets or exceeds functional test criteria
for high quality gel electrophoresis.Application: •Specifically formulated to alleviate the effects of
glycerol in DNA sequencing or PAGE gelsStorage: Ambient. Crystals which form at low
temperatures can be redissolved by warming briefly. May be auto claved.
71949 6bottles
Glycerol Tolerant Gel (GTG) Buffer, 20X Solution
ULTRAPUREMB GradeGlycerol Tolerant Gel (GTG) Buffer is formulated to resolve the problem of gel distortion associated with sequencing samples that contain glycerol. Standard TBE buffer causes gel distortion between 300-500 bases as a result of reactions between glycerol and boric acid to form an anionic ester compound, which migrates in the sequencing gel. This buffer also works equally well with samples that contain no glycerol.Form: Solution consists of 1.78M Tris, 0.57M
Taurine and 0.01M EDTA. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity dH
2O for a solution consisting of 0.089M
Tris, 0.029M Taurine and 0.5mM EDTA. Total volume is 1.5 liters of 20X buffer or 30 liters of 1X buffer.
pH (1:20, 25°C): 8.9 ± 0.1Conductivity 1:20, 830 ± 100 µmhos/cmTesting: Meets or exceeds functional test criteria
for high quality gel electrophoresis. Application: •Specifically formulated to alleviate the effects of
glycerol in DNA sequencing or PAGE gelsStorage: Ambient. Crystals which form in the cold
at low temperatures can be redissolved by warming briefly.
75827 1L
β-Glycerophosphate, Sodium Salt, Pentahydrate
C3H
5(OH)
2PO
4Na
2•5H
2O
Formula Weight: 306.11 (pentahydrate)PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97%Identity: By IRMoisture (KF): 29.4 ± 3% (pentahydrate)Alpha Content: ≤ 2.0%Heavy Metals (Pb): ≤ 0.002%CAS #819-83-0
16365 25gm 100gm 500gm 1kg
-Glycerophosphate, Sodium Salt, Pentahydrate
C3H
5(OH)
2PO
4Na
2•5H
2O
Formula Weight: 306.11Alpha Content: 50%CAS #1555-56-2
21655 100gm 500gm 1kg
Glycinamide, HydrochlorideH
2NCH
2CONH
2•HCl
Formula Weight: 110.54PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRpKa (20°C): 8.20 ± 0.15Moisture: ≤ 5.0%Iron (Fe): ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #1668-10-6
16395 5gm 10gm 25gm 100gm
Biochemicals
Biochemicals
71w w w . u s b w e b . c o m
Glycine
ULTRAPUREMB GradeH
2NCH
2COOH
Formula Weight: 75.07PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRλ
max: 260 nm, 1M, 1 cm: ≤ 0.05
280 nm, 1M, 1 cm: ≤ 0.05Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Readily Carbonizable Substances: Passes testHydrolyzable Substances: Passes testArsenic (As): ≤ 1 ppmBarium (Ba): ≤ 1 ppmCadmium (Cd): ≤ 1 ppmCalcium (Ca): ≤ 5 ppmChloride (Cl): ≤ 50 ppmCopper (Cu): ≤ 1 ppmIron (Fe): ≤ 1 ppmLead (Pb): ≤ 1 ppmMagnesium (Mg): ≤ 1 ppmManganese (Mn): ≤ 1 ppmSulfate (SO
4): ≤ 50 ppm
Zinc (Zn): ≤ 1 ppmContaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testCAS #56-40-6
16407 100gm 500gm 1kg 5kg
GlycineACS Reagent GradeH
2NCH
2COOH
Formula Weight: 75.07PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.002%Chloride (Cl): ≤ 0.005%Sulfate (SO
4): ≤ 0.005%
Ammonium (NH4): ≤ 0.005%
Substances Darkened by Sulfuric Acid: Passes test
Hydrolyzable Substances: Passes test
16372 2.5kg
GlycineUSBioAnalyzedH
2NCH
2CO
2H
Formula Weight: 75.07PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IRLoss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Sulfate (SO
4): ≤ 65 ppm
Heavy Metals (Pb): ≤ 20 ppmChloride (Cl): ≤ 70 ppmHydrolyzable Substances: Passes testResidual Solvents: Meets requirementsCAS #56-40-6
16405 500gm 1kg 5kg 25kg
Glycine, HydrochlorideNH
2CH
2COOH•HCl
Formula Weight: 111.53PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5% (as is)Identity: By IRChromatography (TLC): HomogeneousLoss on Drying: ≤ 3%CAS #6000-43-7
16420 100gm 500gm 1kg
GlycogenSource: Beef LiverCAS #9005-79-2
16440 1gm 5gm 25gm
Glycogen
ULTRAPURESource: OystersPRODUCT SPECIFICATIONSForm: Light tan powderIdentity: By IR[α]20
D : +170° to 200° (c=0.2, H
2O, dry basis)
Solubility (0.25%, 1M NaCl): Passes testLoss on Drying (110°C, 4 hrs.): ≤ 15%CAS #9005-79-2
16445 5gm 10gm 25gm
Glycogen, 20 mg/ml
ULTRAPUREMB GradeSource: OystersPRODUCT SPECIFICATIONSConcentration: 20 mg/ml in nuclease-free waterNote: Glycogen is an inert carrier which
significantly increases the efficiency of extracted DNA and RNA by ethanol precipitation.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testStorage: -20°C
77534 1ml 5x1ml
Glycyl-GlycineNH
2CH
2CONHCH
2COOH
Formula Weight: 132.12PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Chromatography (TLC): HomogeneousIdentity: By IRpKa (25°C): 8.25 ± 0.15Solubility (10%, H
2O): Clear and colorless
Loss on Drying: ≤ 0.2%Chloride (Cl): ≤ 0.02%CAS #556-50-3
16505 100gm 1kg
Bioc
hem
ical
sBiochemicals
7� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Glycyl-Glycine
ULTRAPURENH
2CH
2CONHCH
2COOH
Formula Weight: 132.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Chromatography (TLC): HomogeneousIdentity: By IRpKa (25°C): 8.25 ± 0.15Solubility (10%, H
2O): Clear and colorless
pH (10%, H2O): 5.0 - 7.0
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.2%Chloride (Cl): ≤ 0.02%Sulfate (SO
4): ≤ 0.03%
Iron (Fe): ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmFree glycine content: ≤ 0.02%CAS #556-50-3
16507 50gm 100gm 250gm 1kg
Glycyl-Glycyl-β-Naphthylamide, Hydrobromide
C14
H16
O2N
3Br
Formula Weight: 338.20PRODUCT SPECIFICATIONSForm: White to off-white powderChromatography (TLC): HomogeneousStorage: 4°CCAS #3313-48-2
16676 100mg 500mg 1gm
GMPSee Guanosine-5'-Monophosphate, page 237
GTPSee Guanosine-5'-Triphosphate, page 238
Guanidine, Hydrochloride
ULTRAPUREMB Grade(NH
2)2C=NH•HCl
Formula Weight: 95.53PRODUCT SPECIFICATIONSForm: White/clear crystalsIdentity: By IRAssay: ≥ 99.5%Solubility (6M, H
2O): Clear and colorless
A (225 nm, 6M, 1 cm): ≤ 0.2A (260 nm, 6M, 1 cm): ≤ 0.03Melting Point: 184 ± 3°CResidue on Ignition: ≤ 0.05%Aldehyde: None detectedAmmeline: None detectedArsenic (As): ≤ 1 ppmCopper (Cu): ≤ 1 ppmIron (Fe): ≤ 2 ppmLead (Pb): ≤ 5 ppmSulfate (SO
4): ≤ 50 ppm
Zinc (Zn): ≤ 1 ppmDNase (endo): Passes testRNase: Passes testProtease: Passes testApplications:•Used for extraction of RNA•Preparation of high molecular weight genomic
DNA•Solubilizing inclusion bodiesPhenol/Chloroform RNA Isolation MethodA. Guanidine HCl Homogenization Buffer for RNA
Isolation: 8.0M Guanidine HCl, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25 ml of phenol is required for every 10 ml of sample.
Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Product Code 75829 or 77510).
C. RNA Resuspension Buffer: TE, pH 7.5.CAS #50-01-1
75823 100gm 500gm
Guanidine, Hydrochloride(NH
2)2C=NH•HCl
Formula Weight: 95.53PRODUCT SPECIFICATIONSForm: White/clear crystalsIdentity: By IRMelting Point: 184 - 190°CLoss on Drying: ≤ 0.50%Note: Contains some water insoluble matter.CAS #50-01-1
34007 250gm 1kg 5kg
Guanidine Thiocyanate
ULTRAPURE(NH
2)2C=NH•HNCS
Formula Weight: 118.16PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99%Identity: By IRA (280 nm, 6M, 1 cm): ≤ 0.8Solubility (5%, H
2O): Passes test
Solubility (5%, MeOH): Passes testMelting Point: 120 ± 2°CMoisture (KF): ≤ 1%Copper (Cu): ≤ 0.5 ppmIron (Fe): ≤ 5 ppmLead (Pb): ≤ 1 ppmZinc (Zn): ≤ 2 ppmApplications:•Used to extract RNA from cells and tissue•Denatures protein, thus inactivating
ribonucleasesSolution for Guanidine Thiocyanate RNA
Extraction: Guanidine Thiocyanate 4.0M Dithiothreitol 0.1mM Sodium Acetate, pH 5.2 20mM N-Lauryl sarcosine 0.5% Heat slightly (65°C) to dissolve the guanidine
thiocyanate and sodium acetate. Add the dithiothreitol and N-lauryl sarcosine. The pH should be about 5.5. Filter and store at room temperature.
Cesium Chloride RNA Isolation MethodA. Guanidine Thiocyanate Homogenization Buffer
for RNA Isolation: 4.0M Guanidine Thiocyanate, 0.1M Tris, pH 7.5, 1.0% 2-mercaptoethanol.
B. Cesium Chloride RNA Isolation Solution: Most researchers layer their RNA homogenates onto a cushion of 5.7M Cesium Chloride.
C. RNA Resuspension Buffer: TE, pH 7.5.CAS #593-84-0
75818 100gm 250gm 1kg
Biochemicals
Biochemicals
73w w w . u s b w e b . c o m
GuanineC
5H
5N
5O
Formula Weight: 151.13PRODUCT SPECIFICATIONSForm: White to cream colored powderAssay: 97.0 - 102.0% (dry basis)Identity: By IRLoss on Drying (105°C, 4 hrs.): ≤ 1.5%Residue on Ignition: ≤ 0.2%Chromatography (TLC): HomogeneousArsenic (As): ≤ 2 ppmHeavy Metals (Pb): ≤ 10 ppmParticle Size (passes 160 microns): ≥ 95%
(by weight)Storage: Ambient in tightly closed containers.Reference Constants:λ
max (pH 7, NRC ): 246 nm
εmax
(pH 7, NRC ): 10.7 x 103
ε260
(pH 7, DBR ): 7.2 x 103
CAS #73-40-5
16730 100gm 500gm 1kg
Guanine HydrochlorideC
5H
5N
5O•HCl
Formula Weight: 187.59 (anhydrous)* 205.60 (monohydrate)PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderAssay (pH 1.0): 98 ± 3% (dry basis)Assay (pH 7.0): 98 ± 3% (dry basis)Identity: By IRChromatography (TLC): HomogeneousSpectral Ratios (pH 1.0): A
250/A
260: 1.37 ± 0.03
A280
/A260
: 0.84 ± 0.02Spectral Ratios (pH 7.0): A
250/A
260: 1.42 ± 0.03
A280
/A260
: 1.04 ± 0.02Moisture (KF): ≤ 10%λ
max (pH 1.0): 248 nm ± 1 nm
λmax
(pH 7.0): 246 nm ± 1 nm*Note: The moisture for this product can vary up
to a monohydrate.CAS #635-39-2
16740 10gm 25gm 100gm 1kg
GuanosineC
10H
13N
5O
5
Formula Weight: 283.24PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV): ≥ 98%λ
max: 252 nm ± 1 nm
Spectral Ratios: A
250/A
260: 1.15 ± 0.03
A280
/A260
: 0.67 ± 0.02Loss on Drying: ≤ 12.0%Reference Constants:λ
max (pH 7, NRC ): 253 nm
εmax
(pH 7, NRC ): 13.7 x 103
CAS #118-00-3
16750 10gm 25gm 100gm 1kg
Guanosine, Low EndotoxinC
10H
13N
5O
5•nH
2O
Formula Weight: 283.24•nH2O
PRODUCT SPECIFICATIONSForm: White to slightly yellow crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRMoisture (KF): ≤ 13%Heavy Metals (Pb): ≤ 10 ppmTotal Microbial Count: ≤ 100 cfu/gm Escherichia coli: Negative Pseudomonas aeruginosa: Negative Staphylococcus aureus: Negative Salmonella: NegativeTest for Endotoxins (LAL test): ≤ 1.5 EU/mgCAS #118-00-3
16751 25gm 100gm
Guanosine-2',3'-Cyclic Monophosphate, Sodium Salt(Cyclic GMP)
C10
H11
N5O
7PNa
Formula Weight: 367.19Storage: -20°CCAS #15718-49-7
16762 100mg 250mg
Guanosine-3'-5' Monophosphate, Sodium Salt(cGMP)
C10
H11
N5O
7PNa
Formula Weight: 367.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV): ≥ 95%Spectral Ratios: A
250/A
260: 0.91 ± 0.03
A280
/A260
: 0.71 ± 0.03Storage: -20°CCAS #40732-48-7
16770 250mg
Guanosine-5'-Diphosphate, Disodium Salt(GDP)
C10
H13
N5O
11P
2Na
2•3H
2O
Formula Weight: 541.21Reference Constants:λ
max (pH 7, NRC ): 253 nm
εmax
(pH 7, NRC ): 13.7 x 103
ε260
(pH 7, DBR ): 11.8 x 103
Storage: -20°C, protect from moisture.CAS #43139-22-6
16785 25mg 100mg 500mg 1gm
Guanosine-5'-Monophosphate, Disodium Salt(GMP)
C10
H12
N5O
8PNa
2
Formula Weight: 407.18PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Total UV): ≥ 98%Moisture (KF): ≤ 25%Reference Constants:λ
max (pH 7, NRC ): 252 nm
εmax
(pH 7, NRC ): 13.7 x 103
ε260
(pH 7, DBR ): 11.7 x 103
Storage: 4°CCAS #5550-12-9
16790 5gm
Bioc
hem
ical
sBiochemicals
7� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Guanosine-5'-Triphosphate, Sodium Salt, 100mM Solution(GTP)
ULTRAPUREC
10H
12N
5O
14P
3Na
4
Formula Weight: 611.11Form: 100mM aqueous solution, pH 7.5Storage: -20°C
77243 25µmol(250µl)
Guanosine-5'-Triphosphate, Disodium Salt(GTP)
C10
H14
N5O
14P
3Na
2
Formula Weight: 567.14PRODUCT SPECIFICATIONSAssay: ≥ 95%λ
max: 252 nm ± 1nm
εmax
: (13.7 ± 0.7) x103
Spectral Ratios (pH 7): A
250/A
260: 1.15 ± 0.03
A280
/A260
: 0.65 ± 0.03Moisture (KF): ≤ 10.0%Reference Constants:λ
max (pH 7, NRC ): 252 nm
εmax
(pH 7, NRC ): 13.7 x 103
ε260
(pH 7, DBR ): 11.7 x 103
Storage: -20°C in tightly closed containers.CAS #56001-37-7
16800 25mg 100mg 500mg 1gm
Guanylic AcidSee Guanosine-5'-Monophosphate, page 237
HematoxylinC
16H
14O
6
Formula Weight: 302.28 (anhydrous)CAS #517-28-2
16865 5gm 25gm 100gm
HeminC
34H
32O
4N
4FeCl
Formula Weight: 651.94 Source: Porcine
16880 10gm 50gm
HemoglobinSource: BovineCAS #9008-02-0
16890 200gm 1kg
HemoglobinSource: Bovine Blood2X crystallizedPRODUCT SPECIFICATIONSForm: Brownish-red crystalline powderAssay: ≥ 99%Identity: By IRMoisture (KF): ≤ 3.0%Solubility: Soluble in distilled water or dilute
buffers.Storage: 4°CCAS #9008-02-0
16891 1gm 5gm 10gm
Heparin, Sodium Salt
ULTRAPUREUSBioAnalyzedPRODUCT SPECIFICATIONSForm: White to pale cream-colored powderAssay: ≥ 140 USP Heparin units/mg (dry basis)Identity: Passes testpH (1%, H
2O): 5.0 - 7.5
Loss on Drying: ≤ 5.0%Residue on Ignition: 28.0% to 41.0%Nitrogen Content: 1.3% to 2.5% (dry basis)Protein: Passes testHeavy Metals: ≤ 0.003%Antifactor Xa Activity: 80 - 120%Bacterial Endotoxins: ≤ 0.03 Endotoxin units/
Heparin unitApplication:•Used as a blocking agent to reduce background
in Southern and in situ hybridizationCAS #9041-08-1
16920 50,000units 100,000units 500,000units 1mu
Hexamethonium ChlorideC
12H
30Cl
2N
2•2H
2O
Formula Weight: 309.32PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 98.0% (as dihydrate)Identity: By IR
16950 25gm
Heparin, Sodium SolutionAvailable in 10 ml vials. Sterile filtered,
1,000 units per ml.Not for human use.Storage: 4°C
16925 10vl
Biochemicals
Biochemicals
75w w w . u s b w e b . c o m
HEPES, 1M Solution, pH 7.3
ULTRAPUREMB GradeForm: 1M solution of HEPES in high purity H
2O,
pH 7.3 (at 37°C). Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles.
pH (37°C): 7.3 ± 0.1Contaminant Testing:
DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
16924 100ml 500ml 1L
HEPES(N-[2-Hydroxyethyl]piperazine-N' [2-Ethane-sulfonic Acid])
ULTRAPUREC
8H
18N
2O
4S
Formula Weight: 238.30PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0% (dry basis)Identity: By IRpKa (20°C): 7.55 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.2Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.005%Phosphate (PO
4): ≤ 0.001%
Sulfate (SO4): ≤ 0.01%
Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #7365-45-9
16926 100gm 250gm 1kg 5kg
HEPES, Sodium SaltHO(CH
2)2(C
4H
8N
2)(CH
2)2SO
3Na
Formula Weight: 260.29PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRpKa (20°C): 7.55 ± 0.15pH (0.1M, H
2O): 10.2 ± 0.8
A (250 nm, 5%, 1 cm): ≤ 0.30A (260 nm, 5%, 1 cm): ≤ 0.175Solubility (5%, H
2O): Clear solution
Loss on Drying: ≤ 4.0%Heavy Metals (Pb): ≤ 10 ppmCAS #75277-39-3
16928 25gm 100gm 1kg
HEPPSHO
3S(CH
2)3(C
4H
8N
2)(CH
2)2OH
Formula Weight: 252.33PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 8.00 ± 0.15A (250 nm, 5%, 1 cm): ≤ 0.2Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #16052-06-5
16927 100gm 1kg
n-Hexadecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate
See Sulfobetaine 16 (SB3-16), page 293
Hexokinase(E.C.2.7.1.1)Source: YeastPRODUCT SPECIFICATIONSForm: Lyophilized, ammonium sulfate-free
powderActivity: ≥ 140 units/mg protein ≥ 80 units/mg powderUnit Definition: One unit converts 1 µmol of
glucose to glucose-6-phosphate per min at 25°C, pH 7.5 in the presence of ATP.
Contaminating Activities: PGI: ≤ 0.1% G-6-PDH: ≤ 0.01% PGluM: ≤ 0.01% MK: ≤ 0.01% ATPase: ≤ 0.003% PGDH: ≤ 0.005% CK: ≤ 0.005% GR: ≤ 0.05%Note: The above product is available in
manufacturing quantities as a lyophilized powder or an ammonium sulfate suspension. Please inquire for bulk pricing.
Storage: -20°C, protect from moisture.CAS #9001-51-8
16965 1,000units 2,500units 10,000units 25,000units 50,000units
Hexokinase(E.C.2.7.1.1)Source: YeastPRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionActivity: ≥ 140 units/mg proteinUnit Definition: One unit converts 1 µmol of
glucose to glucose-6-phosphate/min at 25°C, pH 7.5 in the presence of ATP.
Contaminating Activities: PGI: ≤ 0.1% G-6-PDH: ≤ 0.01% PGluM: ≤ 0.01% MK: ≤ 0.01% ATPase: ≤ 0.001% 6-PGDH: ≤ 0.005% CK: ≤ 0.005% GR: ≤ 0.05%Note: The above product is available in
manufacturing quantities as a lyophilized powder or an ammonium sulfate suspension. Please inquire for bulk pricing.
Storage: 4°C
16975 2,500units 10,000units 25,000units 50,000units
Bioc
hem
ical
sBiochemicals
76 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
D-Histidine(C
3N
2H
3)CH
2CH(NH
2COOH)
Formula Weight: 155.15CAS #351-50-8
17055 1gm 10gm 25gm 100gm
D-Histidine, Hydrochloride, Monohydrate
(C3H
3N
2)CH
2CH(NH
2)COOH•HCl•H
2O
Formula Weight: 209.63CAS #6341-24-8
17050 2gm 10gm 25gm 100gm
DL-Histidine(C
3N
2H
3)CH
2CH(NH
2)COOH
Formula Weight: 155.15CAS #4998-57-6
17060 10gm 25gm 100gm
L-Histidine, Free BaseUSBioAnalyzed(C
3N
2H
3)CH
2CH(NH
2)COOH
Formula Weight: 155.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% C
6H
9N
3O
2 (dry basis)
Identity: By IRChromatographic Purity: Passes testpH (2%, H
2O): 7.0 - 8.5
[α]25D : +12.6° to +14.0° (c=11 in 6N HCl)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.4%Heavy Metals (Pb): ≤ 0.0015%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Sulfate (SO
4): ≤ 0.03%
CAS #71-00-1
17070 25gm 100gm 1kg
L-Histidine, Monohydrochloride, Monohydrate
(C3H
3N
2)CH
2CH(NH
2)COOH•HCl•H
2O
Formula Weight: 209.63PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +8.5° to +10.5° (c=in 6N HCl)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.10%Heavy Metals (Pb): ≤ 10 ppmAmmonium (NH
4): ≤ 0.02%
Arsenic (As2O
3): ≤ 1 ppm
Iron (Fe): ≤ 30 ppmSulfate (SO
4): ≤ 0.03%
CAS #5934-29-2
17080 100gm 1kg 5kg
L-Histidine, Monohydrochloride, Monohydrate, Histamine Free
(C3H
3N
2)CH
2CH(NH
2)COOH•HCl•H
2O
Formula Weight: 209.63PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.0%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +8.5 to +10.5° (c=11 in 6N HCl)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmAmmonium (NH
4): ≤ 0.02%
Arsenic (As2O
3): ≤ 1 ppm
Chloride (Cl): 16.6 - 17.1%Iron (Fe): ≤ 10 ppmSulfate (SO
4): ≤ 0.03%
CAS #5934-29-2
17085 100gm 500gm 1kg
L-Histidinol, Dihydrochloride(C
3H
3N
2)CH
2CH(NH
2)CH
2OH•2HCl
Formula Weight: 214.09CAS #1596-64-1
17095 100mg 1gm 5gm
HistoneSource: Calf ThymusStorage: 4°C
17130 100mg 500mg 1gm
Histone, Salt FreeSource: Calf ThymusSalt free, Dialyzed, LyophilizedStorage: 4°C
17105 100mg 500mg 1gm
Histone-Lysine RichSource: Calf ThymusStorage: 4°C
17120 25mg 100mg
Histone-Lysine Rich F1
Source: Calf ThymusStorage: 4°C
17126 10mg 50mg
Histone-Slightly Lysine Rich F2b
Source: Calf ThymusStorage: 4°C
17128 10mg 50mg
DL-Homocysteine, Free Base(2-Amino-4-Mercaptobutyric)
HSCH2CH
2CH(NH
2)COOH
Formula Weight: 135.18CAS #454-29-5
17195 1gm 5gm
DL-HomocystineC
8H
16N
2O
4S
2
Formula Weight: 268.35CAS #870-93-9
17185 25gm 100gm
Biochemicals
Biochemicals
77w w w . u s b w e b . c o m
Horseradish PeroxidaseSee Peroxidase, page 270
Human Pro-matrilysin, Recombinant(r-hProMMP-7)
Source: Human recombinant from E. coliPRODUCT SPECIFICATIONSForm: Solution in 25mM Tris-HCl (pH 7.5),
150mM NaCl, 5mM CaCl2, 0.01% Brij-35 and
0.02% NaN3
Concentration: Approx. 1 mg/mlSpecific Activity: ≥ 1,400 units/mg proteinUnit Definition: One unit is defined as the
digestion of 1 µg Azocoll/min at 37°C.Storage: -80°C. Avoid repeated freezing and
thawing.
26115 100µg
HydrocortisoneUSBioAnalyzedC
21H
30O
5
Formula Weight: 362.46PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 97.0 - 102.0% (dry basis)Chromatographic Purity: Meets requirementsIdentity: By IRIdentity (UV): Passes test[α]20
D : +150° to +156° (c=1 in Dioxane)
Loss on Drying: ≤ 1.0%Residue on Ignition: NegligibleO.V.I.: Meets requirementsCAS #50-23-7
17320 5gm 10gm 25gm 100gm
Hydroxylamine, HydrochlorideNH
2OH•HCl
Formula Weight: 69.49PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRMelting Point: 148 - 160°CLoss On Drying: ≤ 7.0%CAS #5470-11-1
17330 500gm 1kg
Hydroxy-L-Proline, BacterialC
5H
9NO
3
Formula Weight: 131.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0% (as is)Identity: By IRChromatography (TLC): Homogeneous[α]25
D : -74.0° to -77.0° (c=4 in H
2O)
Loss on Drying: ≤ 0.2%
17447
Hydroxy-L-ProlineC
5H
9NO
3
Formula Weight: 131.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0% (as is)Identity: By IRChromatography (TLC): Homogeneous[α]25
D : -74.0° to -77.0° (c=4 in H
2O)
Loss on Drying: ≤ 0.2%CAS #51-35-4
17445 10gm 25gm 100gm 1kg
L-Hydroxysuccinic AcidSee L-Malic Acid, page 252
HydroxyureaCH
4N
2O
2
Formula Weight: 76.05PRODUCT SPECIFICATIONSForm: White crystalline powderChromatography (TLC): HomogeneousIdentity: By IRMelting Point: 139 - 142°CMoisture (KF): ≤ 2.0%Storage: 4°CCAS #127-07-1
17490 1gm 5gm 10gm 25gm
HypoxanthineC
5H
4N
4O
Formula Weight: 136.11PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: 100 ± 3%Chromatography (TLC): HomogeneousIdentity: By IRλ
max (pH 7.0): 250 nm ± 1 nm
εmax
(pH 7.0): [10.7 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 1.32 ± 0.05
A280
/A260
: 0.09 ± 0.02Loss on Drying: ≤ 1.0%Reference Constants:λ
max (pH 7, NRC ): 250 nm
εmax
(pH 7, NRC ): 10.7 x 103
ε260
(pH 6, DBR ): 7.9 x 103
CAS #68-94-0
17520 5gm 25gm 100gm
r-iCDHSee Isocitrate Dehydrogenase, Recombinant,
page 243
Igepal CA-630Nonidet P40 Substitute ([Octylphenoxy]polyethoxyethanol)
ULTRAPUREPRODUCT SPECIFICATIONSForm: Clear, slightly yellow liquidDensity: 1.06 ± 0.005Solubility (5%, H
2O): Complete, clear and
colorlesspH (5%, H
2O): 5.5 - 7.0
(n20d): 1.491 ± 0.001
Chemically indistinguishable from Nonidet P40, which is no longer commercially available.
19626 500ml 1L
Bioc
hem
ical
sBiochemicals
78 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Imidazole
ULTRAPUREC
3H
4N
2
Formula Weight: 68.08PRODUCT SPECIFICATIONSForm: White to cream crystalline flakes/powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (25°C): 6.95 ± 0.15Melting Point: No more than a 3 degree range
which includes 90°CMoisture: ≤ 1.0%Iron (Fe): ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #288-32-4
17525 25gm 100gm 500gm 1kg 5kg
IndigalSee 5-Bromo-3 Indolyl-β-D Galactopyranoside,
page 199
Indole-3-Acetic AcidC
10H
9NO
2
Formula Weight: 175.18Note: This product is for R & D purposes only.
It is not for use as a plant growth regulator in commercial horticulture applications, on developed plants, nor for drug or household uses.
CAS #87-51-4
17550 1gm 5gm 25gm 100gm 500gm
Indole-3-Acrylic AcidC
11H
9NO
2
Formula Weight: 187.19Storage: 4°CCAS #1204-06-4
17565 1gm 5gm 10gm
Indole-3-Butyric AcidC
12H
13NO
2
Formula Weight: 203.24CAS #133-32-4
17575 5gm 25gm 100gm
Indole-3-Butyric Acid, Potassium SaltC
12H
12NO
2K
Formula Weight: 241.33Very soluble in water.
17580 5gm 25gm
InosineC
10H
12N
4O
5
Formula Weight: 268.23PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Total UV): 98.0 - 102.0% (dry basis)Identity: By IRSpectral Ratios (pH 6.0): A
250/A
260: 1.70 ± 0.05
A280
/A260
: 0.25 ± 0.03Loss on Drying: ≤ 1.0%Arsenic (As
2O
3): ≤ 2 ppm
Heavy Metals (Pb): ≤ 10 ppmReference Constants:λ
max (pH 6, NRC ): 249 nm
εmax
(pH 6, NRC ): 12.3 x 103
ε260
(pH 6, DBR ): 7.1 x 103
CAS #58-63-9
17620 5gm 10gm 25gm 100gm
Inositol(myo-Inositol)
C6H
6(OH)
6
Formula Weight: 180.16PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97.0% (dry basis)Identity: By IRMelting Point: 224 - 227°CLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%CAS #87-89-8
17645 100gm 500gm 1kg
InulinSource: Chicory RootPRODUCT SPECIFICATIONSForm: White to off-white crystalline powder[α]20
D : -35° ± 5.0° (c=5 in H
2O)
Moisture (KF): ≤ 10%Residue on Ignition: ≤ 0.2%CAS #9005-80-5
17670 25gm 100gm
Invertase(E.C.3.2.1.26)Source: YeastForm: Solution in 45% glycerolActivity: AOAC K value of 0.3CAS #9001-02-9
17680 100gm 500gm 1kg
5-Iodo-2'-Deoxyuridine(IDU)
C9H
11IN
2O
5
Formula Weight: 354.09Reference Constants:λ
max (pH 7, NRC ): 289 nm
εmax
(pH 7, NRC ): 7.69 x 103
CAS #54-42-2
17735 500mg 1gm 5gm 25gm
Iodonitrotetrazolium Violet(INT)
Grade IC
19H
13ClIN
5O
2
Formula Weight: 505.69CAS #146-68-9
17740 100mg 250mg 500mg 1gm 5gm 25gm
Biochemicals
Biochemicals
79w w w . u s b w e b . c o m
Iodonitrotetrazolium Violet(INT)
Grade IIC
19H
13ClIN
5O
2
Formula Weight: 505.69This grade is possibly less pure than Grade I and
may be less clear in solution but is suitable for enzyme assays.
CAS #146-68-9
17741 1gm 5gm 25gm
Ionomycin, Calcium SaltC
41H
70O
9•Ca
Formula Weight: 747.07PRODUCT SPECIFICATIONSForm: Polyether antibiotic with a high affinity for
Ca2+
Purity (HPLC): ≥ 95%Reference:1. Hu, T. Q. and Weiler, L. (1994) Canadian
Journal of Chemistry 72, 1500-1511.Storage: 4°C, in tight containersCAS #56092-82-1
25106 1mg
Ionomycin, Free AcidC
41H
72O
9
Source: Streptomyces conglobatusFormula Weight: 709.00PRODUCT SPECIFICATIONSForm: Polyether antibiotic with a high affinity for
Ca2+.Storage: 4°C, in tight containersCAS #56092-81-0
25107 1mg
IPTGSee Isopropyl-β-D-Thiogalactopyranoside,
page 244
Isocitrate Dehydrogenase, (NAD+ Specific), Recombinant[r-ICDH (NAD+)]
(E.C.1.1.1.41)Source: Bacterial recombinant in E. coliPRODUCT SPECIFICATIONSForm: Lyophilized powderSpecific Activity: ≥ 40 units/mg proteinContaminating Activity:
Isocitrate Dehydrogenase (NADP+): ≤ 5.0%Unit Definition: One unit converts 1 µmole of
Isocitrate to α-ketoglutarate/min at 25°C, pH 8.5.
Storage: -20°C
26120 150units 600units
Isocitrate Dehydrogenase, (NADP+ Specific), Recombinant[r-ICDH (NADP+)]
NADP+ Specific(E.C.1.1.1.42)Source: Yeast recombinant in S. cerevisiaePRODUCT SPECIFICATIONSForm: 50% Glycerol SolutionSpecific Activity: ≥ 30 units/mg proteinpH: 7.0 ± 0.5Unit Definition: One unit converts 1 µmol of
isocitrate to α-Ketoglutarate/min at 25°C, pH 8.5.
Contaminating Activity: ICDH (NAD+): ≤ 0.5%Note: The above product is available in
manufacturing quantities as an ammonium sulfate suspension or a glycerol solution. Please inquire for bulk pricing.
Storage: -20°C
17798 150units 600units
DL-Isocitric Acid, Trisodium SaltC
6H
5O
7Na
3•nH
2O
Formula Weight: 258.07•nH2O
PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 93%Identity: By IRMoisture (KF): ≤ 20%CAS #1637-73-6
17810 1gm 5gm 10gm
DL-IsoleucineCH
3CH
2CH(CH
3)CH(NH
2)COOH
Formula Weight: 131.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRChromatography (TLC): HomogeneousLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Heavy Metals: ≤ 20 ppmChloride (Cl): ≤ 0.02%Iron (Fe): ≤ 50 ppmSulfate (SO
4): ≤ 0.05%
CAS #443-79-8
17820 10gm 25gm 100gm 500gm 1kg
L-IsoleucineUSBioAnalyzedCH
3CH
2CH(CH
3)CH(NH
2)COOH
Formula Weight: 131.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% C
6H
13NO
2 (dry basis)
Identity: By IRChromatographic Purity: Passes testpH (1%, H
2O): 5.5 - 7.0
[α]20D : +38.9° to +41.8° (c=4 in 6N HCl, 20°C)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.3%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 0.0015%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Residual Solvents: Meets requirementsCAS #73-32-5
17825 5gm 10gm 25gm 100gm 500gm 1kg
Isonicotinic AcidC
6H
5NO
2
Formula Weight: 123.11CAS #55-22-1
17872 100gm 500gm
Bioc
hem
ical
sBiochemicals
80 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Isonicotinic Acid HydrazideC
6H
7N
3O
Formula Weight: 137.14CAS #54-85-3
17870 50gm 100gm 500gm
Isopropyl-β-D-Thiogalactopyranoside (IPTG), Dioxane Free
ULTRAPUREC
9H
18O
5S
Formula Weight: 238.30PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 99%Identity: By IR[α]20
D : -31.5°C ± 1° (c=1 in H
2O)
Melting Point: 109 - 114°CMoisture (KF): ≤ 2.0%Dioxane (GC): ≤ 0.02%Applications:•Induces the lac operon in E. coli by binding and
inactivating the lac repressor•Selection for lac Y mutants•Selection for recombinant plasmids containing β-galactosidase fusion proteins
•Induces the cellular content of lactose permeaseStorage: -20°CCAS #367-93-1
17886 1gm 5gm
Isopropyl-β-D-Thiogalactopyranoside (IPTG), - Plant Origin, Dioxane-Free
C9H
18O
5S
Formula Weight: 238.30PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: (HPLC) ≥ 98%Identity: By IR[α]20
D : -31.5° ± 1.5° (c=1 in H
2O)
Melting Point: 109 - 114°CMoisture (KF): ≤ 2.0%Dioxane (GC): ≤ 0.02%Storage: -20°C, desiccated; protect from lightCAS #367-93-1
17913 1gm 5gm
Kanamycin SulfateUSBioAnalyzedC
18H
36N
4O
11•H
2SO
4
Formula Weight: 582.58PRODUCT SPECIFICATIONSForm: Off-white powderActivity: ≥ 750 µg of C
18H
36N
4O
11/mg powder (dry basis)
≥ 720 µg of C18
H36
N4O
11/mg powder (as is)
Identity (A, B, C): Passes testsChromatographic Purity: Passes testpH (1%, H
2O): 6.5 - 8.5
Loss on Drying: ≤ 4.0%Residue on Ignition: ≤ 1.0%Crystallinity: Passes testMode of Action:•Binds to ribosomal components and inhibits
protein synthesis. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.
Application:•A selectable marker for cosmid vectors used in
transfection of mammalian cellsStorage: 4°CCAS #25389-94-0
17924 5gm 25gm
KeratinCAS #68238-35-7
17925 100gm 500gm
KethoxalC
6H
12O
4
Formula Weight: 148.16PRODUCT SPECIFICATIONSForm: Clear, light yellow liquidIdentity: By IRStorage: -20°CCAS #27762-78-3
17930 5gm 10gm 25gm
-Ketoglutaric Acid, Free AcidC
5H
6O
5
Formula Weight: 146.10PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 98.0% (as is)Identity: By IRMelting Point: 114 - 119°CLoss on Drying: ≤ 1.0%Storage: 4°CCAS #328-50-7
17950 25gm 100gm 500gm 1kg
α-Ketoglutaric Acid, Disodium Salt, Dihydrate
C5H
4O
5Na
2•2H
2O
Formula Weight: 226.09 (dihydrate) 190.06 (anhydrous)PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0% (as is)Identity: By IRMoisture: 14.0 - 18.0%CAS #305-72-6
17955 5gm 25gm 100gm 1kg
KinetinC
10H
9N
5O
Formula Weight: 215.21PRODUCT SPECIFICATIONSForm: Off-white powderAssay: ≥ 96%Identity: By IRε
max: ≥ 18.0 x 103
λmax
: 267 ± 1 nmMoisture (KF): ≤ 2.0%Reference Constants:λ
max (pH 6, NRC ): 267 nm
εmax
(pH 6, NRC ): 18.8 x 103
Storage: -20°CCAS #525-79-1
17980 500mg 1gm
Biochemicals
Biochemicals
81w w w . u s b w e b . c o m
LactalbuminCAS #9013-90-5
18035 1lb 5lb 25lb 100lb
Lactalbumin Hydrolysate, Enzymatic Hydrolysate(Peptones)
Form: Light tan powderMoisture: ≤ 6.5%pH (6%, H
2O): 6.7 - 7.7
Amino Nitrogen: 4.5 - 6.0%Total Nitrogen: 11.0 - 13.0%Protein (N x 6.38): 70.0 - 83.0%Microbial Content: Standard Plate Count: ≤ 5,000 cfu/gm Yeast and Mold: ≤ 100 cfu/gm Coliform: ≤ 10 cfu/gm Salmonella: None detected in 100 gmCAS #68458-87-7
18055 1lb 5lb 25lb
Lactate Dehydrogenase(E.C.1.1.1.27)Source: Beef HeartPRODUCT SPECIFICATIONSForm: Lyophilized powder, salt freeActivity: Approx. 250 units/mg proteinUnit Definition: One unit reduces 1 µmol of
pyruvate to L-lactate per minute at 37°C, pH 7.0.
Contaminating Activities: PK: ≤ 0.01% GPT: ≤ 0.01% MDH: ≤ 0.05% GOT: ≤ 0.01%Storage: -20°CCAS #9001-60-9
18065 5,000units 10,000units 25,000units 50,000units 100,000units
Lactate Dehydrogenase(E.C.1.1.1.27)Source: Bovine HeartPRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionUnit Definition: One unit oxidizes 1 µmol of NADH/
min at 25°C, pH 7.4.Storage: 4°C
18066 5,000units 10,000units 25,000units 50,000units 100,000units
Lactate Dehydrogenase(E.C.1.1.1.27)Source: Porcine HeartPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 300 units/mg proteinProtein: ≥ 30%Unit Definition: One unit oxidizes 1 µmol of NADH/
min at 25°C, pH 7.0.Contaminating Activities: MDH: ≤ 0.03% MK: ≤ 0.01% PK: ≤ 0.003% GPT*: ≤ 0.03% GOT*: ≤ 0.03% *Both transaminase activities include α-
hydroxyglutarate dehydrogenase activity.Note: The above product is available in
manufacturing quantities as a lyophilized powder, ammonium sulfate suspension, or a glycerol solution. Please inquire for bulk pricing.
Storage: -20°C
18073 5,000units 10,000units 50,000units 100,000units
Lactate Dehydrogenase(E.C.1.1.1.27)Source: Rabbit MusclePRODUCT SPECIFICATIONSForm: Lyophilized, ammonium sulfate-free
powderActivity: ≥ 400 units/mg proteinUnit Definition: One unit oxidizes 1 µmol of NADH/
min at 25°C, pH 7.4.Contaminating Activities: PK: ≤ 0.01% MDH: ≤ 0.01% GPT: ≤ 0.01%Storage: -20°CCAS #9001-60-9
18074 5,000units 10,000units 25,000units 50,000units 100,000units
Lactate Dehydrogenase, Recombinant(r-LDH)
(E.C.1.1.1.27)Source: Chicken HeartHost cell: E. coliPRODUCT SPECIFICATIONSForm: Lyophilized powderSpecific Activity: ≥ 200 units/mg proteinProtein (per mg of powder): 600 ± 300 µgSucrose (per mg of powder): 400 ± 300 µgPotassium Phosphate (per mg of powder): 100
± 100 µgContaminating Activities: Malate Dehydrogenase: ≤ 0.030% Myokinase: ≤ 0.010% Pyruvate Kinase: ≤ 0.010% Glutamic-Pyruvic Transaminase (GPT)*: ≤
0.030% Glutamic-Oxaloacetic Transaminase (GOT)*: ≤
0.030% *Both transaminase activities include α-
hydroxyglutarate dehydrogenase activityUnit Definition: One unit is defined as 1 µmol of
NAD+ production per minute under the assay conditions (25°C, pH 7.0).
Storage: -20°C, protect from moisture
26112 1,000units
DL-Lactic Acid, Free Acid, 85%Form: Clear, viscous liquidCAS #598-82-3
18140 500gm 1kg
Bioc
hem
ical
sBiochemicals
8� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
DL-Lactic Acid, Lithium Salt(Lithium Lactate)
Reagent GradeC
3H
5LiO
3
Formula Weight: 96.01
18115 100gm 500gm 1kg
DL-Lactic Acid, Sodium Salt, 60% Solution(Sodium Lactate)
USBioAnalyzedC
3H
5NaO
3
Formula Weight: 112.06PRODUCT SPECIFICATIONSForm: Odorless, clear liquidAssay: 58.8 - 61.2%pH (as is soln.): 5.0 - 9.0[α]25
D : 0.00° ± 0.05 (as is solution)
Citric, oxalic, phophoric, or tartaric acid: Passes test
Methanol & methyl esters: Passes testSugars: Passes testSulfate (SO
4): Passes test
Heavy Metals (Pb): ≤ 10 ppmChloride (Cl): ≤ 0.05%Iron (Fe): ≤ 1 ppm
18085 1lb
L- (+)-Lactic Acid 40%, Free AcidC
3H
6O
3
Formula Weight: 90.08
18170 5gm 10gm 25gm
L- (+)-Lactic Acid, Lithium Salt(Lithium Lactate)
C3H
5LiO
3
Formula Weight: 96.01PRODUCT SPECIFICATIONSForm: White crystalline powder[α]20
D : -11.5° ± 1.5° (c=10, H
2O)
Solubility: Passes testLoss on Drying: ≤ 0.5%CAS #27848-80-2
18160 5gm 10gm 100gm
LactoferrinSource: Bovine MilkStorage: 4°C
18177 100mg 500mg 1gm
β-LactoglobulinSource: Bovine MilkStorage: 4°CCAS #9045-23-2
18180 1gm
D-(+)-Lactose, MonohydrateUSBioAnalyzedC
12H
22O
11•H
2O
Formula Weight: 360.31PRODUCT SPECIFICATIONSForm: White powderClarity and Color of Solution: Passes testIdentity (A, B, C): Passes tests[α]20
D : +54.4° to +55.9° (c=10 in 0.2 ml 6N
NH4OH/100 ml H
2O)
Acidity or Alkalinity: Passes testLoss on Drying: ≤ 0.5%Moisture (KF): 4.5 - 5.5%Residue on Ignition: ≤ 0.1%Protein and Light-Absorbing Impurities: Passes
testHeavy Metals (Pb): ≤ 5 ppmMicrobial Limits: Passes testCAS #64044-51-5
18185 1kg 5kg
Lambda DNASee Molecular Biology Products & Kits Section,
page 92
Lauryl MaltosideSee Dodecyl Maltoside, page 222
N-Lauroyl Sarcosine, Sodium Salt(Sodium-N-Lauryl Sarcosine)
ULTRAPUREHO
2CCH
2CH
3NCO(CH
2)10
CH2•Na
Formula Weight: 293.38PRODUCT SPECIFICATIONSForm: White to faint white-yellow powderIdentity: By IRMoisture (KF): ≤ 5.0%pH (10%, H
2O): 7.5 - 8.5
Solubility (10%, H2O): Clear to slightly hazy,
colorless to faint yellow solutionCAS #137-16-6
21653 50gm 100gm 500gm 1kg
Lauryl Sulfate, Lithium SaltSee Lithium Dodecyl Sulfate, page 249
Lauryl Sulfate, Sodium SaltSee Sodium Dodecyl Sulfate, page 286
LB Agar, Ready-Made PowderForm: Luria-Bertani Agar. Off-white free flowing
powder.pH (3.5%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 10 gm/L Yeast Extract: 5 gm/L Sodium Chloride: 5 gm/L Agar: 15 gm/LTo use: Dissolve 35 gm powder per liter of
purified water. Autoclave, cool and make plates.
Application: •For the propagation of bacteria
75851 250gm 1kg
Biochemicals
Biochemicals
83w w w . u s b w e b . c o m
LB Broth, Ready-Made PowderForm: Luria-Bertani Broth. Off-white free flowing
powder.pH (2.0%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 10 gm/L Yeast Extract: 5 gm/L Sodium Chloride: 5 gm/LTo use: Dissolve 20 gm powder per liter of
purified water. Autoclave.Application: •For the propagation of bacteria
75852 250gm 1kg
r-LDHSee Lactate Dehydrogenase, Recombinant,
page 245
LecithinSource: Egg YolkCAS #8002-43-5
18225 25gm 100gm
LecithinSource: SoybeanPRODUCT SPECIFICATIONSForm: Off-white to light tan granular powderMoisture: ≤ 1.5% (at time of packaging)Acetone Insolubles: ≥ 95.0%Hexane Insolubles: ≤ 0.3%CAS #8002-43-5
18245 500gm 1kg
Leptomycin A
25109
Leptomycin BC
33H
48O
6
Formula Weight: 540.73Form: Leptomycin B is an antifungal antibiotic
that blocks CRM1 mediated nuclear export of proteins containing Nuclear Export Signal (NES). It inactivates CRM1 by covalently modifying the sulfhydryl residue of Cysteine-529. This inhibits the formation of a complex between CRM1, Ran, and a protein with a NES. This results in the nuclear retention of the NES-bearing protein(1). Inhibiting the CRM1-mediated nucleo-cytoplasmic export of proteins bearing a NES produces the nuclear accumulation of a large number of proteins(2) such as Actin, c-Abl, Cdc25, Cyclin B1, HDM2, HIV-1, IΚBα and p53.
References1. Kudo, N., Matsumori, N., Taoka, H., Fujiwara,
D., Schreiner, E. P., Wolff, B., Yoshida, M. and Horinouchi, S. (1999) Proc. Natl. Acad. Sci. USA 96, 9112-9117.
2. Lain, S., Xirodimas, D. and Lane, D. (1999) Experimental Cell Research 253, 315-324.
CAS #87081-35-4
25110 1µg
D-LeucineC
6H
13O
2N
Formula Weight: 131.17CAS #328-38-1
18265 5gm 25gm
DL-Leucine(CH
3)2CHCH
2CH(NH
2)COOH
Formula Weight: 131.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (as is)Identity: By IRChromatography (TLC): HomogeneousLoss on Drying: ≤ 0.3%CAS #328-39-2
18270 25gm 100gm 1kg
L-LeucineC
6H
13NO
2
Formula Weight: 131.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5%Identity: By IR[α]20
D : +14.5° to +16.5° (c=4 in 6N HCl dry
basis)Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Lead (Pb): ≤ 10 ppmHeavy Metals (Pb): ≤ 20 ppmCAS #61-90-5
18280 50gm 100gm 1kg 5kg
Leucine Aminopeptidase(E.C.3.4.11.1)Form: Suspension in 70% saturated ammonium
sulfateUnit Definition: One unit hydrolyzes 1 µmol of
L-leucinamide/min at 25°C, pH 8.5.Storage: 4°C CAS #9001-61-0
18260
L-Leucyl-glycylglycineC
10H
19N
3O
4
Formula Weight: 245.28PRODUCT SPECIFICATIONSForm: White to off-white powderIdentity: By IRChromatography (TLC): HomogeneousStorage: -20°C, protect from moisture
18350 100mg 500mg 1gm 5gm
Bioc
hem
ical
sBiochemicals
8� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Leupeptin
ULTRAPUREC
20H
38N
6O
4•1⁄2 H
2SO
4•H
2O
Formula Weight: 493.61Form: White powder[α]20
D : -82.3° ± 3.0°(c=0.2, H
2O)
Moisture (KF): ≤ 4.0%Applications:•Leupeptin, a protease inhibitor, will
strongly inhibit Trypsin, Papain, Plasmin, Thrombokinase, Kallikrein and Cathepsin B. The half-maximal inhibitory concentration ranges from 0.5 to 75 µg/ml, depending on the enzyme and the substrate.
•Leupeptin does not inhibit Chymotrypsin, Elastase, Renin, or Pepsin.
Storage: -20°CCAS #55123-66-5
18413 1mg 5mg 25mg 100mg
Lima Bean Trypsin InhibitorSource: Lima BeanForm: Lyophilized, salt freeActivity: One mg inhibits at least 2.2 mg Trypsin.Storage: 4°C
18430 100mg 500mg 1gm
Linoleic AcidC
18H
32O
2
Formula Weight: 280.45PRODUCT SPECIFICATIONSForm: Clear, colorless oilAssay: ≥ 99.5% (By GC)Storage: -20°C, vacuum sealed ampules, under
inert gas.CAS #60-33-3
18455 1gm 5gm 10gm 25gm
Linoleic Acid Methyl EsterC
19H
34O
2
Formula Weight: 294.47PRODUCT SPECIFICATIONSForm: Clear, colorless liquidPurity: ≥ 99.0%Storage: 4°CCAS #112-63-0
18465 1gm 5gm
Lipase(E.C.3.1.1.3)Source: Porcine PancreasPRODUCT SPECIFICATIONSForm: Powder purified by modification of method
of Verger, et al., (1969) Biochem. Biophys. Acta, 188, 272. Standardized with Lactose.
Activity: Approx. 100 units/mg protein, standardized to 16 units/mg powder
Unit Definition: One unit liberates 100 µmol of fatty acid per hour from olive oil emulsion at 37°C, pH 7.8.
Contaminating Activity: α-Amylase: ≤ 0.05 units/mg powderStorage: 4°C
18480 1gm 5gm 10gm
Lipase(E.C.3.1.1.3)Source: Porcine PancreasPRODUCT SPECIFICATIONSForm: Stabilized powderActivity: ≥ 24 USP units/mgLoss on Drying: ≤ 6.0%Unit Definition: One unit liberates 1 µmol of fatty
acid/min from olive oil at 37°C, pH 9.0.
18485 100gm 500gm
Lipase(E.C.3.1.1.3)Source: Chromobacterium viscosumPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 400 units/mg proteinProtein: 500 ± 250 µg/mg powderKCl: 500 ± 250 µg/mg powderUnit Definition: One unit liberates 1 µmol of fatty
acid/min at 37°C, pH 7.0.Storage: -20°CCAS #9001-62-1
18492 25,000units 50,000units 100,000units
DL- -Lipoic Acid(DL-6,8-Thioctic Acid)
C8H
14O
2S
2
Formula Weight: 206.33PRODUCT SPECIFICATIONSForm: Light yellow crystalline powderAssay: ≥ 98.0% (dry basis)Identity: Passes testLoss on Drying: ≤ 0.5%Storage: In tight, light-resistant containersCAS #1077-28-7
18505 1gm 5gm 25gm 50gm 100gm
Lipoxidase(E.C.1.13.11.12)Source: SoybeanForm: Lyophilized powderUnit Definition: One unit causes an increase
in the extinction at 234 nm of 0.001/min at 25°C, pH 9.0.
Storage: -20°CCAS #9029-60-1
18510 200mg 1gm
Lissamine Rhodamine B-200C
27H
29N
2O
6S
2Cl
Formula Weight: 577.11PRODUCT SPECIFICATIONSForm: Purple powderForm: Fluorescent protein stain at a
concentration of 5% on CeliteCAS #3520-42-1
18515 1gm 5gm
Lithium Chloride (LiCl), 7.5M Solution
ULTRAPUREMB GradeForm: 7.5M solution of lithium chloride (LiCl) in
high purity dH2O. Solution is 0.2 µm filtered
and dispensed into durable, square Nalgene bottles.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75902 100ml
Biochemicals
Biochemicals
85w w w . u s b w e b . c o m
Lithium ChlorideACS Reagent GradeLiClFormula Weight: 42.39PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99%Insoluble Matter: ≤ 0.01%Titratable Base: ≤ 0.008 meq/gLoss on Drying : ≤ 1.0%Nitrate (NO
3): ≤ 0.001%
Sulfate (SO4): ≤ 0.01%
Barium (Ba): ≤ 0.003%Heavy Metals (Pb): ≤ 0.002%Iron (Fe): ≤ 0.001%Calcium (Ca): ≤ 0.01%Potassium (K): ≤ 0.01%Sodium (Na): ≤ 0.20%CAS #7447-41-8
18516 500gm 2.5kg
Lithium ChlorideReagent GradeLiClFormula Weight: 42.39PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99%Loss on Drying: ≤ 0.6%Insoluble (in HCI): ≤ 0.005%Alkalinity (as Li
2CO
3): ≤ 0.03%
Sodium Chloride (NaCI): ≤ 0.015%Calcium Chloride (CaCI
2): ≤ 0.03%
Iron Oxide (Fe2O
3): ≤ 0.002%
Sulfate (SO4): ≤ 0.006%
CAS #7447-41-8
18527 100gm 500gm
Lithium Dodecyl Sulfate(Lithium Lauryl Sulfate)
ULTRAPURECH
3(CH
2)11
OSO3Li
Formula Weight: 272.33PRODUCT SPECIFICATIONSAssay (acidity): ≥ 98%Assay (C
12 by GC): ≥ 98%
A (230 nm, 3%, 1 cm): ≤ 0.2A (280 nm, 3%, 1 cm): ≤ 0.1Loss on Drying: ≤ 1%Chloride (Cl): ≤ 0.01%Copper (Cu): ≤ 5 ppmIron (Fe): ≤ 1 ppmLead (Pb): ≤ 5 ppmCAS #2044-56-6
32816 5gm 25gm 100gm
Lithium LactateSee DL-Lactic Acid-Lithium Salt or L-(+)-Lactic
Acid Lithium Salt, page 246
Lithium Lauryl SulfateSee Lithium Dodecyl Sulfate, page 249
LitmusPRODUCT SPECIFICATIONSForm: Dark blue granulespH (0.01%, H
2O): 7 - 10
Visual Transition Interval: pH 8.3 - Blue pH 4.5 - RedCAS #1393-92-6
18525 25gm 100gm
Liver Desiccated-DefattedSource: BovineCAS #8002-47-9
18533 1lb 5lb 25lb 100lb
D-Luciferin
ULTRAPUREC
11H
8O
3S
2N
2
Source: SyntheticFormula Weight: 280.32PRODUCT SPECIFICATIONSForm: Lyophilized crystalline powderStorage: -20°C, protect from moisture.CAS #2591-17-5
18567 1mg 5mg 50mg
Luminol(3-Aminophthalhydrazide)
C8H
7N
3O
2
Formula Weight: 177.16PRODUCT SPECIFICATIONSForm: Off-white to yellowish powderAssay: ≥ 98%Identity: By IRMoisture (KF): ≤1%Solubility (2%, 1N NaOH): Passes testResidue on Ignition: ≤ 2%
18574 5gm 25gm
Luria Agar, Ready-Made PowderForm: Off-white free flowing powderpH (4.0%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 10 gm/L Yeast Extract: 5 gm/L Sodium Chloride: 10 gm/L Agar: 15 gm/LTo use: Dissolve 40 gm powder per liter of
purified water. Autoclave, cool and make plates.
Application: •For the propagation of bacteria
75853 250gm 1kg
Bioc
hem
ical
sBiochemicals
86 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Luria Broth, Ready-Made PowderForm: Off-white free flowing powderpH (2.5%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 10 gm/L Yeast Extract: 5 gm/L Sodium Chloride: 10 gm/LTo use: Dissolve 25 gm powder per liter of
purified water. Autoclave.Application: •For the propagation of bacteria
75854 250gm 1kg
Luteinizing Hormone Releasing Factor LRF
C55
H75
N17
O13
Formula Weight: 1182.29Source: Synthetic PeptidePyroglu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-
NH2
CAS #33515-09-2
18566 1mg 5mg 25mg
L-Lysine, MonohydrateC
6H
14N
2O
2•H
2O
Formula Weight: 164.20PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +25.5° to +27.0° (c=8 in 6N HCl)
Moisture (KF): 10.0 to 11.5%Heavy Metals: ≤ 10 ppmStorage: -20°CCAS #39665-12-8
18581 5gm 100gm
D-Lysine, HydrochlorideNH
2(CH
2)4CH(NH
2)COOH•HCl
Formula Weight: 182.65CAS #7274-88-6
18575 500mg 1gm 5gm 10gm 25gm
DL-Lysine, HydrochlorideNH
2(CH
2)4CH(NH
2)COOH•HCl
Formula Weight: 182.65Form: White crystalline powderAssay: ≥ 98%Identity: By IRChromatography (TLC): HomogeneousCAS #70-53-1
18580 25gm 100gm 1kg
L-Lysine, DihydrochlorideNH
2(CH
2)4CH(NH
2)COOH•2HCl
Formula Weight: 219.11PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): Homogeneous[α]20
D : +15° to +17° (c=2 in H
2O)
CAS #657-26-1
18595 25gm 100gm
L-Lysine, HydrochlorideNH
2(CH
2)4CH(NH
2)COOH•HCl
Formula Weight: 182.65PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IR[α]20
D : +19.0 to +21.5° (c=8 in 6N HCl)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmAmmonium (NH
4): ≤ 0.02%
Arsenic (As2O
3): ≤ 1 ppm
Iron (Fe): ≤ 20 ppmSulfate (SO
4): ≤ 0.03%
CAS #657-27-2
18585 1kg 5kg 10kg
Lysozyme
ULTRAPURE(E.C.3.2.1.17)Source: Chicken Egg WhitePRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: Approximately 20,000 units/mgUnit Definition: One unit causes a decrease in
absorbance of 0.001 per min at 450 nm, 25°C and pH 7.0 with Micrococcus lysodeikticus as a substrate.
Moisture: ≤ 6.0%Chloride: 3.2 - 4.2%pH (2%, H
2O): 3.0 - 4.0
Storage: -20°C, desiccated
18645 1gm 5gm 10gm 25gm 100gm
M13mp8, Single-Stranded DNASee Molecular Biology Products & Kits Section,
page 92
M13mp18, Single-Stranded DNASee Molecular Biology Products & Kits Section,
page 92
Magnesium Chloride (MgCl2), 1M Solution
ULTRAPUREMB GradeForm: 1M solution of magnesium chloride (MgCl
2)
in high purity dH2O. Solution is 0.2 µm filtered
and dispensed into vials and/or durable, square Nalgene bottles.
Contaminant Testing: Heavy Metals(Pb): ≤ 5 ppm DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
78641 10x1ml 100ml
Biochemicals
Biochemicals
87w w w . u s b w e b . c o m
Magnesium Chloride, Hexahydrate
ULTRAPUREACS Reagent GradeMgCl
2•6H
2O
Formula Weight: 203.30PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 102.0%Insoluble Matter: ≤ 0.005%Nitrate (NO
3): ≤ 0.001%
Phosphate (PO4): ≤ 5 ppm
Sulfate (SO4): ≤ 0.002%
Ammonium (NH4): ≤ 0.002%
Barium (Ba): ≤ 0.005%Calcium: ≤ 0.01%Manganese (Mn): ≤ 5 ppmPotassium (K): ≤ 0.005%Sodium (Na): ≤ 0.005%Strontium (Sr): ≤ 0.005%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmCAS #7791-18-6
18641 500gm 1kg
Magnesium Sulfate, HeptahydrateACS Reagent GradeMgSO
4•7H
2O
Formula Weight: 246.47PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 102.0%pH (5%, H
2O, 25°C): 5.0 - 8.2
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 5 ppmNitrate (NO
3): ≤ 0.002%
Ammonium (NH4): ≤ 0.002%
Calcium (Ca): ≤ 0.02%Manganese (Mn): ≤ 5 ppmPotassium (K): ≤ 0.005%Sodium (Na): ≤ 0.005%Strontium (Sr): ≤ 0.005%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmCAS #10034-99-8
18651 500gm 1kg 5kg
Magnesium Sulfate, HeptahydrateUSBioAnalyzedMgSO
4•7H
2O
Formula Weight: 246.47PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5% (anhydrous by ignition)Identity: Passes testspH (5%, H
2O): 5.0 - 9.2
Loss on Ignition: 40.0 - 52.0%Chloride (Cl): ≤ 0.014%Iron (Fe): ≤ 20 ppmHeavy Metals (Pb): ≤ 0.001%Selenium (Se) : ≤ 0.003%O.V.I.: Meets the requirements
18664 100gm 500gm 2.5kg
Malate DehydrogenaseSource: Candida utilis(E.C.1.1.1.37)PRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 1,000 units/mg proteinContaminating Activities: NADH Oxidase: ≤ 0.001% Glutamate Dehydrogenase: ≤ 0.001% LDH : ≤ 0.01% Fumarase: ≤ 0.01% GOT: ≤ 0.01%Storage: -20°C desiccated
18677
Malate Dehydrogenase, Lyophilized(E.C.1.1.1.37)Source: Porcine Heart (Mitochondrial)PRODUCT SPECIFICATIONSForm: Lyophilized powder, ammonium sulfate freeActivity: ≥ 1,100 units/mg protein.Protein: 10 - 50%Unit Definition: One unit causes the oxidation of
1 µmol of NADH/min at 25°C, pH 7.4.Contaminating Activities: Fumarase (L-Malate): ≤ 0.01% LDH: ≤ 0.01% GOT: ≤ 0.01% GDH (NAD): ≤ 0.002% NADH Oxidase: ≤ 0.001%Note: The above product is available in
manufacturing quantities as a lyophilized powder, an ammonium sulfate suspension or a glycerol solution. Please inquire for bulk pricing.
Storage: -20°CCAS #9001-64-3
18670 5,000units 15,000units 25,000units 50,000units 100,000units
Malate Dehydrogenase, Suspension(L-Malate:NAD Oxidoreductase)
(E.C.1.1.1.37)Source: Porcine Heart (Mitochondrial)PRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionActivity: ≥ 1,500 units/mg proteinUnit Definition: One unit causes the oxidation of
1 µmol of NADH/min at 25°C, pH 7.4.Contaminating Activities: Fumarase (L-Malate): ≤ 0.001% LDH (NADH): ≤ 0.001% GOT: ≤ 0.001% GDH (NAD): ≤ 0.001% Creatine Kinase: ≤ 0.001% GPT: ≤ 0.001%Note: The above product is available in
manufacturing quantities as a lyophilized powder, an ammonium sulfate suspension, or a glycerol solution. Please inquire for bulk pricing.
Storage: 4°C, do not freeze
18665 5,000units 15,000units 25,000units 50,000units 100,000units
Bioc
hem
ical
sBiochemicals
88 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Malate Dehydrogenase, Recombinant(r-MDH)
(E.C.1.1.1.37)Host cell: E.coliPRODUCT SPECIFICATIONSForm: Lyophilized powder, ammonium sulfate freeSpecific Activity: ≥ 200 units/mg proteinUnit Definition: One unit is defined as 1 µmol of
NAD+ production per minute under the assay conditions (25°C, pH 7.5).
Protein (per mg of powder): 300 ± 200 µgPotassium Phosphate (per mg of powder): 700
± 200 µgContaminating Activities: Fumarase (L-Malate): ≤ 0.010% Lactate Dehydrogenase: ≤ 0.010% Glutamate Dehydrogenase: ≤ 0.010% NADH Oxidase: ≤ 0.001% Glutamic-Oxaloacetic Transaminase (GOT)*: ≤ 0.001%
*GOT activity includes α-hydroxyglutarate dehydrogenase activity
Storage: -20°C, protect from moisture
26113 1,000units
Maleic Acid(Toxilic Acid)
Reagent GradeC
4H
4O
4
Formula Weight: 116.07PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0% (dry basis)Identity: By IRSolubility (50%, H
2O): Clear and colorless
Moisture (KF): ≤ 2.0%Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #110-16-7
18685 500gm 1kg
DL-Malic AcidHOOCCH(OH)CH
2COOH
Formula Weight: 134.09PRODUCT SPECIFICATIONSForm: White granular powderAssay: ≥ 98% (as is)Identity: By IRMoisture (KF): ≤ 1.0%CAS #617-48-1
18695 1kg
L-Malic AcidC
4H
6O
5
Formula Weight: 134.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (GC): ≥ 99%Identity: By IR[α]20
D : -26.5° to -30.0° (c=5.5 in pyridine)
Melting Point: 102 - 106°CMoisture (KF): ≤ 1.0%CAS #97-67-6
18700 100gm 500gm 1kg
Malonic Acid, Free Acid(Propanedioic Acid)
C3H
4O
4
Formula Weight: 104.06CAS #141-82-2
18705 100gm 250gm 500gm 1kg
MaltaseSee α-D-Glucosidase, page 231
Maltose, MonohydrateHigh Purity GradeC
12H
22O
11•H
2O
Formula Weight: 360.31PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98%Identity: By IR[α]20
D : +130.4° ± 1.3° (c=4 in H
2O, NH
4OH)
Solubility (10%, H2O): Passes test
Loss on Drying: ≤ 6%Residue on Ignition: ≤ 0.05%Dextrins: Passes testArsenic (As): ≤ 0.5 ppmIron (Fe): ≤ 5 ppmHeavy Metals (Cu): ≤ 10 ppmCAS #6363-53-7
18724 100gm 500gm 1kg
D-(+)-Maltose, MonohydrateC
12H
22O
11•H
2O
Formula Weight: 360.31PRODUCT SPECIFICATIONSForm: White powder[α]20
D : +130.0° ± 2.0° (c=4 in H
2O, NH
3)
Loss on Drying: ≤ 6% (to constant wt. at 100°C and not more than 0.5mm Hg)
Water-Insoluble Material: Passes testDextrins: Passes testResidue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmIron (Fe): ≤ 5 ppmArsenic (As): ≤ 0.5 ppmCAS #6363-53-7
18726 100gm 500gm 1kg
D-(+)-Maltose, MonohydrateC
12H
22O
11•H
2O
Formula Weight: 360.31PRODUCT SPECIFICATIONSForm: White to off-white powderMaltose Content: ≥ 92.0% (dry basis)Glucose Content: ≤ 3.0% (dry basis)Loss on Drying: ≤ 7.0%Heavy Metals (Pb): ≤ 5 ppmCAS #6363-53-7
18725 500gm 1kg 5kg
MaltotrioseC
18H
32O
16
Formula Weight: 504.44 PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 97%Identity: By IR[α]20
D : +165 ± 1° (c=3 in H
2O)
Moisture: ≤ 5%
18730 1gm 5gm 25gm
Biochemicals
Biochemicals
89w w w . u s b w e b . c o m
DL-Mandelic Acid(DL-α-Hydroxyphenylacetic Acid)
C8H
8O
3
Formula Weight: 152.15CAS #90-64-2
18745 100gm 500gm
Manganese Chloride (MnCl2), 1M Solution
ULTRAPUREMB GradeForm: 1M solution of manganese chloride (MnCl
2)
in high purity dH2O. Solution is 0.2 µm filtered
and dispensed into vials and/or durable, square Nalgene bottles.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
75887 10x1ml 100ml
Manganese Chloride, Tetrahydrate
ULTRAPUREACS Reagent GradeMnCl
2•4H
2O
Formula Weight: 197.91PRODUCT SPECIFICATIONSForm: Pale pink crystalline powderAssay: 98.0 - 101.0%pH (5%, H
2O, 25°C): 3.5 - 6.0
Insoluble Matter: ≤ 0.005%Sulfate (SO
4): ≤ 0.005%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmCalcium (Ca): ≤ 0.005%Magnesium (Mg): ≤ 0.005%Potassium (K): ≤ 0.01%Sodium (Na): ≤ 0.05%Zinc (Zn): ≤ 0.005%CAS #13446-34-9
18753 100gm 250gm
Manganese Sulfate MonohydrateACS Reagent GradeMnSO
4•H
2O
Formula Weight: 169.02PRODUCT SPECIFICATIONSForm: Pink to almost white crystalline powderAssay 98.0 - 101.0%Loss on Ignition: 10.0 - 12.0%Substances Reducing Permanganate: Passes testInsoluble Matter: ≤ 0.01%Chloride (Cl): ≤ 0.005%Calcium (Ca): ≤ 0.005%Magnesium (Mg): ≤ 0.005%Nickel (Ni): ≤ 0.02%Potassium (K): ≤ 0.01%Sodium (Na): ≤ 0.05%Zinc (Zn): ≤ 0.005%Heavy Metals (Pb): ≤ 0.002%Iron (Fe): ≤ 0.002%CAS #10034-96-5
18757 100gm 500gm 2.5kg
MannitolUSBioAnalyzedC
6H
14O
6
Formula Weight: 182.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 96.0 - 101.5% (dry basis)Identity: Passes test[α]25
D : +137° to +145° (c=1 in USP prep.)
Melting Point: 164 - 169°CLoss on Drying: ≤ 0.3%Acidity: Passes testReducing Sugars: Passes testChloride (Cl): ≤ 0.007%Sulfate (SO
4): ≤ 0.01%
Arsenic (As): ≤ 1 ppmCAS #69-65-8
18755 500gm 1kg 5kg
D-(+)-MannoseC
6H
12O
6
Formula Weight: 180.16PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : +14.2° ± 0.7° (c=4 in H
2O, NH
3)
Loss on Drying: ≤ 0.5%CAS #3458-28-4
18775 25gm 100gm 500gm
Matrilysin, Mature, Recombinant(r-hMMP-7)
Source: Human recombinant from E. coliPRODUCT SPECIFICATIONSForm: Suspension in 10mM HEPES (pH 7.4),
5mM CaCl2, 150mM NaCl
Concentration: Approx. 2 mg/mlSpecific Activity: ≥ 3,100 units/mg proteinMolecular Weight: 19,130 Da calculated from
the amino acid sequence and 20 kDa from SDS-PAGE
Unit Definition: One unit is defined as the digestion of 1 µg Azocoll/min at 37°C.
Storage: -80°C
26114 100µg
r-MbSee Myoglobin, Recombinant, page 260
MBTHSee 3-Methyl-2-Benzothiazolinone Hydrazone,
Hydrochloride, Monohydrate, page 255
r-MDHSee Malate Dehydrogenase, Recombinant,
page 252
Mega-8(N-Octanoyl-N-Methylglucamin)
C15
H31
NO6
Formula Weight: 321.42PRODUCT SPECIFICATIONSForm: White powderAssay (HPLC): ≥ 98%Identity: By IRLoss on Drying: ≤ 0.05%pH (1%, H
2O): 5.0 ± 0.5
Storage: 4°C
18786 1gm 5gm 25gm
Bioc
hem
ical
sBiochemicals
90 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Mega-9(N-Nonanoyl-N-Methylglucamin)
C16
H33
NO6
Formula Weight: 335.44PRODUCT SPECIFICATIONSForm: White powderAssay (HPLC): ≥ 99%Identity: By IRLoss on Drying: ≤ 0.5%pH (1%, H
2O): 5 - 8
Storage: 4°C
18787 1gm 5gm 25gm
α-D-(+)-Melibiose, MonohydrateC
12H
22O
11•H
2O
Formula Weight: 360.31PRODUCT SPECIFICATIONSForm: White powder[α]20
D : +135.0° ± 2.0° (c=4 in H
2O)
Loss on Drying: ≤ 5.1%Residue on Ignition: ≤ 0.1%Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #585-99-9
18805 5gm 10gm 25gm 100gm
Menadione(2-Methyl-1,4-Naphthoquinone; Vitamin K
3 )
USBioAnalyzedC
11H
8O
2
Formula Weight: 172.18PRODUCT SPECIFICATIONSForm: Bright yellow powderAssay: 98.5 - 101.0% (dry basis)Identity (A, B): Passes testsMelting Range: 105 - 107°CLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Ordinary Impurities: Passes testCAS #58-27-5
18815 25gm 100gm 500gm
Menadione, Sodium BisulfiteC
11H
9NaO
5S•3H
2O
Formula Weight: 330.29 (trihydrate)PRODUCT SPECIFICATIONSForm: White powderChromatography (TLC): Purity greater than 98%
as compared to reference material.Identity: By IRMoisture (KF): 9.0 - 13.0%Solubility (1%, H
2O): Clear and colorless
Storage: -20°C, protect from lightCAS #130-37-0
18820 50gm 100gm 1kg
Menhaden Fish OilSee Fish Oil, Menhaden, page 227
Mercaptoacetic AcidSee Thioglycolic Acid, Sodium Salt, page 297
β-Mercaptoethylamine, Hydrochloride(Cysteamine HCl; Decarboxycysteine)
HSCH2CH
2NH
2•HCl
Formula Weight: 113.61CAS #156-57-0
18825 10gm 25gm 100gm
MES, Anhydrous(2-(N-Morpholino) Ethane Sulfonic Acid)
C6H
13NO
4S
Formula Weight: 195.24CAS #4432-31-9
18888 100gm 250gm 1kg
MES, Monohydrate(2-(N-Morpholino) Ethane Sulfonic Acid)
ULTRAPUREHO
3S(CH
2)2(C
4H
8NO)•H
2O
Formula Weight: 213.25PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 6.15 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.1Moisture (KF): ≤ 11%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmSolubility (1M, H
2O, 100 ml): Clear and colorless
Note: This product varies up to a monohydrate.CAS #4432-31-9
18886 25gm 100gm 1kg
MES, Sodium Salt(2-(N-Morpholino) Ethane Sulfonic Acid, Sodium Salt)
C6H
12NO
4SNa
Formula Weight: 217.22CAS #71119-23-8
18893 100gm 250gm 1kg
D-MethionineCH
3S(CH
2)2CH(NH
2)COOH
Formula Weight: 149.21PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IR[α]25
D : -22.9° to -24.7° (c=2 in 6N HCl)
Loss on Drying: ≤ 0.2%CAS #348-67-4
18905 5gm 25gm 100gm
Biochemicals
Biochemicals
91w w w . u s b w e b . c o m
DL-MethionineCH
3SCH
2CH
2CH(NH
2)COOH
Formula Weight: 149.21PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals(Pb): ≤ 20 ppmChloride (Cl): ≤ 0.020%Iron (Fe): ≤ 10 ppmSulfate (SO
4): ≤ 0.050%
CAS #59-51-8
18910 200gm 1kg 5kg 10kg 25kg
L-MethionineUSBioAnalyzedC
5H
11NO
2S
Formula Weight: 149.21PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IRpH (1%, H
2O): 5.6 - 6.1
[α]25D : +22.4° to +24.7° (c=2 in 6N HCl)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 0.0015%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Chromatographic Purity: Passes testO.V.I.: Meets requirementsCAS #63-68-3
18915 25gm 100gm 1kg 5kg
Methotrexate(Amethopterin; 4-Amino-10-Methyl Folic Acid)
C20
H22
N8O
5•nH
2O
Formula Weight: 454.44•nH2O
PRODUCT SPECIFICATIONSForm: Yellow/orange crystalline powderIdentity: By IRAssay (306 nm, 0.1N HCl): ≥ 96.0% (dry basis)Assay (259 nm, 0.1N NaOH): ≥ 96.0% (dry basis)Assay (HPLC): ≥ 98.0%Residue on Ignition: ≤ 0.1%Moisture (KF): ≤ 12.0%Application: •Used as a competitive inhibitor of Dihydrofolate
Reductase (DHFR)Storage: 4°CCAS #59-05-2
18970 25mg 100mg 500mg 1gm
3-Methyl-2-Benzothiazolinone Hydrazone, Hydrochloride, Monohydrate(MBTH)
C8H
9N
3S•HCl•H
2O
Formula Weight: 233.72PRODUCT SPECIFICATIONSForm: Slightly yellow crystalline powderAssay: ≥ 98%Identity: By IRCAS #38894-11-0
19025 5gm 10gm 25gm
Methylene Blue, Trihydrate(Methylthionine Chloride)
(C.I.52015)C
16H
18ClN
3S•3H
2O
Formula Weight: 373.90PRODUCT SPECIFICATIONSForm: Dark green crystalline powderAssay: 98.0 - 103.0% (dry basis)Identity: By IRλ
max: 664 nm ± 1
Solubility (1%, H2O): Passes test
pH (1%, H2O): 3.6 - 4.5
Moisture (KF): 8.0 - 18.0%Application:•Used in staining RNA that is bound to nylon
and nitrocellulose membranes. Methylene blue is the stain of choice in visualizing filter bound RNA over a pre-transfer gel stain with ethidium bromide. Ethidium bromide saturates the nucleic acids and reduces the efficiency of RNA transfer.
Typical Procedure:1. Soak the dried filter in 5% acetic acid at room
temperature for 15 minutes.2. Place the filter in 0.5M sodium acetate (pH
5.2) and 0.04% methylene blue for 5-10 minutes at room temperature.
3. Rinse the filter in water for 5-10 minutes.CAS #61-73-4
19220 25gm 100gm 500gm
N,N'-Methylene-bis-Acrylamide(bis-Acrylamide)
ULTRAPUREMB Grade(CH
2=CHCONH)
2CH
2
Formula Weight: 154.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.9%Identity: By IRA (290 nm, 1%, 1 cm): ≤ 0.2pH (1%, H
2O): ≥ 5.5
Acrylic acid: ≤ 0.02%Lead (Pb): ≤ 1 ppmGel Performance: Passes testApplication:•Cross-linking agent used in forming
polyacrylamide gels. The porosity of the gel is determined by the chain length and the degree of cross-linking. Most sequencing gels use a ratio of 1 molecule of cross-linker for every 19 monomers of acrylamide.
CAS #110-26-9
75821 25gm 50gm 100gm 1kg
Bioc
hem
ical
sBiochemicals
9� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Methyl- -D-Glucopyranoside(α-Methyl-D-Glucoside)
C7H
14O
6
Formula Weight: 194.18PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : +156° to +160° (c=10 in H
2O)
Loss on Drying: ≤ 1%CAS #97-30-3
19065 100gm 500gm
Methyl-β-D-Glucopyranoside(Methyl-β-D-Glucoside)
C7H
14O
6
Formula Weight: 194.18PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : -33.7° to -34.7° (c=10 in H
2O, dry basis)
Moisture (KF): ≤ 5.0%CAS #709-50-2
19066 5gm 10gm 50gm
Methyl Green, Zinc Chloride Salt(C.I.42590)C
27H
35Cl
2N
3•ZnCl
2
Formula Weight: 608.81Applications:•For differentiation of DNA and RNA(1)
•A DNA stain in electrophoresis(2)
References:1. Kurnick, N. B., (1952) Stain Technol., 27,
233-242.2. Cutting, J. A. and Roth, T. F., (1973) Anal.
Biochem., 54, 386-394.CAS #7114-03-6
32818 25gm
Methyl-α-D-MannopyranosideC
7H
14O
6
Formula Weight: 194.18CAS #617-04-9
19115 10gm 25gm 100gm
2-Methyl-1,4-NapthoquinoneSee Menadione, page 254
Methyl OrangeACS Reagent GradeC
14H
14N
3NaO
3S
Formula Weight: 327.33PRODUCT SPECIFICATIONSForm: Dark orange powderClarity of Solution: Passes testVisual Transition Interval: pH 3.2 - Pink pH 4.4 - YellowCAS #547-58-0
19150 25gm 100gm
Methyl Paraben(p-Hydroxybenzoic Acid Methyl Ester)(Methyl-p-Hydroxybenzoate)
C8H
8O
3
Formula Weight: 152.15PRODUCT SPECIFICATIONSForm: White powderIdentity: By IRMelting Point: 125 - 128°CMoisture (KF): ≤ 0.5%CAS #99-76-3
19160 100gm 1kg
Methyl Red, Sodium SaltWater solublePRODUCT SPECIFICATIONSForm: Dark orange powderInsoluble Matter: Passes testVisual Transition Interval: pH 4.2 - Pink pH 6.2 - YellowCAS #845-10-3
19165 25gm 100gm
5-Methyl-2'-Deoxycytidine-5'-Monophosphate, Disodium Salt(5-Methyl dCMP)
C10
H14
N3O
7PNa
2
Formula Weight: 365.19 (anhydrous)PRODUCT SPECIFICATIONSForm: White powderAssay (HPLC, as nucleotide): ≥ 98%Assay (HPLC, as nucleoside): ≥ 98%Moisture (KF): ≤ 16%Phosphorylated Nucleosides:≤ 0.20% dCMP≤ 0.049% for any other phosphorylated
nucleosideStorage: -20°C, protect from moisture
19184 100mg
4-Methylumbelliferone(7-Hydroxy-4-Methylcoumarin, Hymecromone)
C10
H8O
3
Formula Weight: 176.17Storage: -20°CCAS #90-33-5
19211 5gm
4-Methyl Umbelliferyl AcetateC
12H
10O
4
Formula Weight: 218.21PRODUCT SPECIFICATIONSForm: White crystalline flakesIdentity: By IRChromatography (TLC): HomogeneousMoisture: ≤ 1.0%Storage: -20°C
19203 5gm 10gm
4-Methylumbelliferyl-α-L-Iduronide, Sodium Salt
C16
H15
NaO9
Formula Weight: 374.27Storage: -20°C
33230 5mg
Biochemicals
Biochemicals
93w w w . u s b w e b . c o m
4-Methylumbelliferyl-N-Acetyl- -D-Galactosaminide(4-Methylumbelliferyl-2-Acetamido-2-Deoxy-β-D-Galactopyranoside)
C18
H21
NO8
Formula Weight: 379.36Storage: -20°CCAS #36476-29-6
33216 25mg 100mg 250mg 1gm
4-Methylumbelliferyl-N-Acetyl-β-D-Glucosaminide(4-Methylumbelliferyl-2-Acetamido-2-Deoxy-β-D-Glucopyranoside)
C18
H21
NO8
Formula Weight: 379.36Storage: -20°CCAS #37067-30-4
33200 25mg 100mg 250mg 1gm
2'-(4-Methylumbelliferyl)-α-D-N-Acetylneuraminic Acid, Sodium Salt
C21
H24
NNaO11
Formula Weight: 489.41Storage: -20°CCAS #76204-02-9
33202
4-Methylumbelliferyl-α-L-Arabinopyranoside
C15
H16
O7
Formula Weight: 308.28Storage: -20°CCAS #69414-26-2
33204 25mg 100mg 1gm
4-Methylumbelliferyl Caprylate(Octanoic Acid 4-Methylumbelliferyl Ester)
C18
H22
O4
Formula Weight: 302.36Storage: -20°CCAS #20671-66-3
19204
4-Methylumbelliferyl- -D-CellobiosideC
22H
28O
13
Formula Weight: 500.45Storage: -20°CCAS #72626-61-0
33206 100mg 500mg
4-Methylumbelliferyl-α-L-Fucopyranoside
C16
H18
O7
Formula Weight: 322.31Storage: -20°CCAS #54322-38-2
33218 5mg 10mg 25mg
4-Methylumbelliferyl-β-D-Fucopyranoside
C16
H18
O7
Formula Weight: 322.31Storage: -20°CCAS #55487-93-9
33222 10mg 25mg
4-Methylumbelliferyl-β-L-Fucopyranoside
C16
H18
O7
Formula Weight: 322.31Storage: -20°CCAS #72601-82-2
33224 10mg
4-Methylumbelliferyl-α-D-Galactopyranoside
C16
H18
O8
Formula Weight: 338.31Storage: -20°CCAS #38597-12-5
33209 25mg 100mg 250mg
4-Methylumbelliferyl- -D-Galactopyranoside
C16
H18
O8
Formula Weight: 338.31Storage: -20°CCAS #6160-78-7
19209 250mg 1gm 5gm
4-Methylumbelliferyl-α-D-Glucopyranoside
C16
H18
O8
Formula Weight: 338.31Storage: -20°CCAS #17833-43-1
33212 25mg 100mg 250mg 1gm
4-Methylumbelliferyl-β-D-Glucopyranoside
C16
H18
O8
Formula Weight: 338.31Storage: -20°CCAS #18997-57-4
33214 250mg 500mg 1gm 5gm
4-Methylumbelliferyl-β-D-Glucuronide Dihydrate(MUG)
C16
H16
O9•2H
2O
Formula Weight: 388.32PRODUCT SPECIFICATIONSForm: White to off-white powderIdentity: By IRChromatography (TLC): HomogeneousMelting Point (Fisher-Johns): 142-150°CMoisture (KF): 9.6-14.8%Storage: -20°C, desiccated, protected from lightCAS #6160-80-1
19208 100mg 500mg 1gm
Bioc
hem
ical
sBiochemicals
9� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
4-Methylumbelliferyl Myo-Inositol-1-Phosphate, N-Methyl-Morpholine Salt
C16
H19
O11
P•C5H
11NO
Formula Weight: 519.44Storage: -20°C
33290
4-Methylumbelliferyl-α-D-Mannopyranoside
C16
H18
O8
Formula Weight: 338.31Storage: -20°CCAS #28541-83-5
33226 10mg 25mg 100mg
4-Methylumbelliferyl-β-D-Mannopyranoside
C16
H18
O8
Formula Weight: 338.31Storage: -20°CCAS #67909-30-2
33220 10mg 25mg 100mg 250mg
4-Methylumbelliferyl Nonanoate(Nonanoic Acid 4-Methylumbelliferyl Ester)
C19
H24
O4
Formula Weight: 316.39Storage: -20°CCAS #18319-93-2
33291
4-Methylumbelliferyl Palmitate(Palmitic Acid 4-Methylumbelliferyl Ester)
C26
H38
O4
Formula Weight: 414.58Storage: -20°CCAS #17695-48-6
33292
4-Methylumbelliferyl Phosphate, Free Acid
C10
H9O
6P
Formula Weight: 256.15Storage: -20°CCAS #3368-04-5
23006
4-Methylumbelliferyl Phosphate, di(2-Amino-2-Methyl-1,3-Propanediol) Salt
C10
H9O
6P•2 C
4H
11NO
2
Formula Weight: 466.42Storage: -20°CCAS #107475-10-5
33293
4-Methylumbelliferyl Phosphate, Dicyclohexylammonium Salt
C10
H9O
6P•2C
6H
13N
Formula Weight: 454.50Storage: -20°CCAS #128218-53-1
33294
4-Methylumbelliferyl Phosphate, Dilithium Salt
C10
H7O
6PLi
2
Formula Weight: 268.01Storage: -20°CCAS #125328-83-8
33295
4-Methylumbelliferyl Phosphate, Disodium Salt
C10
H7O
6PNa
2•nH
2O
Formula Weight: 300.12•nH2O
Storage: -20°CCAS #22919-26-2
19202 250mg 500mg 1gm
4-Methylumbelliferyl- -D-Xylopyranoside
C15
H16
O7
Formula Weight: 308.28Storage: -20°CCAS #6734-33-4
33228 25mg 100mg 250mg 1gm
Methyl VioletPRODUCT SPECIFICATIONSForm: Green crystalline powderInsoluble Matter: Passes testVisual Transition Interval: pH 1.40 - Blue pH 0.80 - Blue-green pH 0.20 - Yellow-greenCAS #8004-87-3
19210 100gm 500gm
DL-Mevalonic Acid LactoneC
6H
10O
3
Formula Weight: 130.14Storage: -20°CCAS #674-26-0
19230 1gm 5gm 10gm
Micrococcus LysodeikticusForm: Sprayed dried cells suitable as lysozyme
substrate
19240 5gm
Biochemicals
Biochemicals
95w w w . u s b w e b . c o m
B2 Microglobin, Recombinant(r-B2M)
Source: Human recombinantFormula Weight: 11,762, determined by amino
acid sequencePRODUCT SPECIFICATIONSForm: Suspension in phosphate-buffered saline
(pH 7.4) containing 0.05% NaN3
Concentration: 1 mg/mlPurity: Single band at about 12k, as determined
by SDS-PAGE under reducing conditionsStorage: 4°C for frequent use, -20°C for long
term storage
26000 500µg 1mg
Milk Powder-Whole
19242 5lb 25lb
Mineral Oil
ULTRAPUREMB GradePRODUCT SPECIFICATIONSForm: Clear, colorless oilIdentity: By IRSpecific Gravity (25°C): 0.818 to 0.880Viscosity (40°C): ≤ 33.5 centistokesNeutrality: Passes testReadily Carbonizable Substances: Passes testLimit of Polynuclear Compounds: Passes testSolid Paraffin: Passes testUse Test: Functionally tested for use in PCRCAS #8042-47-5
71600 10ml 25ml 1L
r-hMMP-7See Matrilysin, Mature, Recombinant, page 253
Monosodium GlutamateSee Glutamic Acid, Monosodium Salt, page 232
MOPS(3-[N-Morpholino]propanesulfonic Acid)
ULTRAPUREHO
3S(CH
2)3(C
4H
8NO)
Formula Weight: 209.26PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 7.20 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.1Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.01%Sulfate (SO
4): ≤ 0.02%
Iron (Fe): ≤ 5 ppmLead (Pb): ≤ 10 ppmCAS #1132-61-2
19256 50gm 100gm 1kg
MOPS Sodium SaltC
7H
14NO
4SNa
Formula Weight: 231.25PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 7.20 ± 0.15A (260 nm, 1M, 1 cm): ≤ 0.1Loss on Drying: ≤ 0.5%Chloride (Cl): ≤ 0.01%Sulfate (SO
4): ≤ 0.02%
Iron (Fe): ≤ 5 ppmLead (Pb): ≤ 10 ppm
19268 100gm 250gm 1kg
MTT(3-[4,5-Dimethyl-2-Thiazolyl]-2,5-Diphenyl Tetrazolium Bromide; Thiazolyl Blue Tetrazolium Bromide)
ULTRAPUREC
18H
16BrN
5S
Formula Weight: 414.32PRODUCT SPECIFICATIONSForm: Yellow powderChromatography (TLC): HomogeneousIdentity: By IRSolubility: Passes testSensitivity: Passes testMelting Point: ≥ 192°CApplications:•Used in the quantitation of DNA synthesis•Detects living and growing cells. MTT is cleaved
by active mitochondria to form a dark blue formazan product.
CAS #298-93-1
19265 250mg 500mg 1gm 5gm 10gm
MTT Formazan(3-[4,5-Dimethyl Thiazolyl-2]-2,5-Diphenyl Formazan)
C18
H17
N5S
Formula Weight: 335.43CAS #57360-69-7
19260 100mg 1gm
MucinSource: Bovine Submaxillary GlandsForm: Dry powder by the method of Nisizawa and
Pigman, (1959) Arch. Ora. Biol., 1, 161.Substrate for neuraminidase.Storage: 4°C
19275 100mg 500mg
MyoglobinSource: Equine HeartPRODUCT SPECIFICATIONSForm: Red-brown lyophilized powderAssay: (SDS Electrophoresis): ≥ 90%Moisture: ≤ 10%Iron: ≥ 0.2%Storage: -20°C, desiccatedCAS #100684-32-0
19285 250mg 1gm
Bioc
hem
ical
sBiochemicals
96 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Myoglobin, Recombinant(r-Mb)
Source: Human Recombinant from E. coliFormula Weight: 11,670 for mature form and
17,801 for immature form, determined by amino acid sequence
PRODUCT SPECIFICATIONSForm: Suspension in phosphate-buffered saline
(pH 7.4) containing 0.05% NaN3.
Concentration: 2 mg/mlPurity: Single band at about 17.5k, as determined
by SDS-PAGE under reducing conditions; the mature and immature forms rMb are indistinguishable from each other
Storage: 4°C, Do not freeze
26003 1mg 5mg
Myo-InositolSee Inositol, page 242
Myristic Acid(Tetradecanoic Acid)
CH3(CH
2)12
COOHFormula Weight: 228.38CAS #544-63-8
19310 100gm 500gm
β-NADSee Nicotinamide Adenine Dinucleotide, page 262
β-NADH Inhibitor(1,6-Dihydronicotinamide-Adenine Dinucleotide)
Source: YeastForm: This product is an isomer of NADH which
greatly depresses the reduction rates of dehydrogenases.
PRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 80 units/mg powderUnit Definition: One inhibitory unit is the activity
of 1 ml of reaction mixture required to reduce LDH isoenzyme-4H activity to 50%.
Storage: -20°C
19321 1mg 10mg
NADHSee β-Nicotinamide Adenine Dinucleotide,
Reduced, page 262
NADPSee Nicotinamide Adenine Dinucleotide
Phosphate, page 263
NADPHSee Nicotinamide Adenine Dinucleotide
Phosphate, Reduced, page 263
Nalidixic AcidC
12H
12N
2O
3
Formula Weight: 232.24PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: 98.0 - 102.0% (dry basis)Assay: 97.5 - 102.0% (as is)Identity: By IRMelting Point: 225 - 231°CLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.2%Mode of Action: Inhibits DNA synthesis by inhibiting DNA
gyraseCAS #389-08-2
19436 5gm 25gm 100gm
α-Naphthalene Acetic AcidC
12H
10O
2
Formula Weight: 186.21CAS #86-87-3
19326 25gm 100gm 1kg
Naphthol AS-MX-AcetateC
21H
19NO
3
Formula Weight: 333.39PRODUCT SPECIFICATIONSForm: Tan powderAssay (HPLC): ≥ 97%Identity: By IRSolubility (1%, Ethylacetate): Clear solutionStorage: 4°C
19378 200mg 1gm 5gm
Naphthol AS-MX-PhosphateC
19H
18NO
5P
Formula Weight: 371.33PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98%Identity: By IRMoisture (KF): ≤ 1.0%Storage: 4°C
19835 500mg 5gm
Naphthol Blue BlackSee Amido Black 10B, page 189
β-Naphthoxyacetic Acid, Free AcidC
12H
10O
3
Formula Weight: 202.21CAS #120-23-0
19400 100gm 1kg
α-Naphthyl AcetateC
12H
10O
2
Formula Weight: 186.21Essentially α-Naphthol freeStorage: -20°CCAS #830-81-9
19331 5gm 10gm 25gm
β-Naphthyl AcetateEssentially β-Naphthol freeC
12H
10O
2
Formula Weight: 186.21Storage: -20°CCAS #1523-11-1
19419 5gm 10gm
α-Naphthyl ButyrateC
14H
14O
2
Formula Weight: 214.26Storage: 4°CCAS #3121-70-8
19332 1gm 5gm 10gm
Biochemicals
Biochemicals
97w w w . u s b w e b . c o m
N-1-Naphthylethylene-Diamine, Dihydrochloride
C12
H14
N2•2HCl
Formula Weight: 259.17Colorimetric determination of Leucine
AminopeptidaseCAS #1465-25-4
19415 10gm 25gm 100gm
α-Naphthyl Phosphate, Monosodium Salt
C10
H8NaO
4P•H
2O
Formula Weight: 264.15 (monohydrate)PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97% (as is monohydrate)Chromatography (TLC): ≥ 99%Identity: By IRMoisture (KF): ≤ 10.0%Storage: 4°CCAS #1136-89-6
19391 5gm 25gm
NBTSee p-Nitro Blue Tetrazolium Chloride, page 263
NDSB-195(Dimethyl Ethyl Ammonium Propane Sulfonate)
C7H
17NO
3S
Formula Weight: 195.28PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98%Identity: By IRSolubility (10%, H
2O): Clear and complete
Moisture (KF): ≤ 1.0%
26216 5gm 25gm 100gm
NDSB-201(3-(1-Pyridino)-1-Propanesulfonate)
C8H
11NO
3S
Formula Weight: 201.25PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98%Identity: By IRSolubility (10%, H
2O): Clear and complete
Melting Point: 275 - 279°CMoisture (KF): ≤ 1.0%pH (50%, H
2O): 2.5 - 6.0
26217 5gm 25gm 100gm
Neocuproine, Hydrochloride(2,9-Dimethyl-1,10-Phenanthroline HCl)
C14
H12
N2•HCl•nH
2O
Formula Weight: 244.72•nH2O
PRODUCT SPECIFICATIONSForm: Off-white to pale greenish-tan powderAssay: ≥ 98.0% (dry basis)Identity: By IRChromatography (TLC): HomogeneousMoisture (KF): ≤ 10%Storage: 4°CCAS #7296-20-0
19430 5gm 10gm 25gm 100gm 1kg
Neomycin Sulfate
ULTRAPUREUSBioAnalyzedC
23H
46N
6O
13•3H
2SO
4
Formula Weight: 908.88PRODUCT SPECIFICATIONSForm: White powderActivity: ≥ 600 µg neomycin/mg powder
(dry basis)Activity: ≥ 552 µg neomycin/mg powder (as is)Identity (A, B, C): Passes testspH (33 mg/ml): 5.0 - 7.5Loss on Drying: ≤ 8.0%Mode of Action: Binds to ribosomal components
and inhibits protein synthesis.Stock Solution: 10 mg/ml in waterWorking Concentration: 10 µg/mlStorage: Ambient, in tight, light resistant
containers.CAS #1405-10-3
19435 5gm 10gm 25gm
Neuraminidase(E.C.3.2.1.18)Source: Clostridium perfringensPRODUCT SPECIFICATIONSForm: Lyophilized, partially purifiedActivity: ≥ 0.5 units/mg materialUnit Definition: One unit releases 1 µmol of sialic
acid/min from bovine submaxillary mucin at 37°C, pH 5.0.
Storage: 4°CCAS #9001-67-6
19455 10mg
Neutral RedC
15H
17N
4Cl
Formula Weight: 288.78PRODUCT SPECIFICATIONSForm: Green-black crystalline powderIdentity: By IRVisual Transition Interval: pH 6.8 - Red pH 8.0 - AmberCAS #553-24-2
19465 25gm 100gm 1kg
NiacinSee Nicotinic Acid, page 263
Niacinamide(Nicotinic Acid Amide; Nicotinamide)
USBioAnalyzedC
6H
6N
2O
Formula Weight: 122.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity (A, B): Passes testsMelting Point: 128 - 131°CLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals: ≤ 0.003%Readily Carbonizable Substances: Passes testResidual Solvents: Meets requirementsCAS #98-92-0
19480 200gm 500gm 1kg 10kg
Bioc
hem
ical
sBiochemicals
98 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Nicacinamide, HydrochlorideC
6H
6N
2O•HCl
Formula Weight: 158.59
19485 500gm
NicotinamideSee Niacinamide, page 261
β-Nicotinamide Adenine Dinucleotide(β-NAD; Coenzyme I; Diphosphopyridine Nucleotide; DPN)
C21
H27
N7O
14P
2•3H
2O
Formula Weight: 717.47 (trihydrate) 663.43 (anhydrous)PRODUCT SPECIFICATIONSForm: White powderAssay (Enzymatic): 99 ± 4%Assay: 100 ± 3% (UV, 260 nm, pH 7.5)Identity: By IRε
340 (pH 10.0): [6.3 ± 0.2] x 103
ε260
(pH 7.5): [18.0 ± 0.5] x 103
Spectral Ratios (pH 7.5): A
250/A
260: 0.83 ± 0.03
A280
/A260
: 0.21 ± 0.02Spectral Ratios (pH 10, reduced with ADH): A
340/A
260 (red): 0.43 ± 0.01
Moisture: ≤ 8%Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°C, protect from moisture.CAS #53-84-9
15335 500mg 1gm 5gm 10gm 25gm 100gm
-Nicotinamide Adenine Dinucleotide, Low Alcohol Content(βNAD)
C21
H27
N7O
14P
2•3H
2O
Formula Weight: 717.47 (trihydrate) 663.43 (anhydrous)PRODUCT SPECIFICATIONSForm: White powderAssay: 99 ± 4% (dry basis) (calc. from A
340,
enzymatic, pH 10.0) 99 ± 3% (dry basis) (calc. from ε
260,
pH 7.5)Identity: By IRε
260 (pH 7.5): [18.0 ± 0.5] x 103
ε340
(pH 10.0): [6.3 ± 0.2] x 103
Spectral Ratios (pH 7.5): A
250/A
260: 0.83 ± 0.03
A280
/A260
: 0.21 ± 0.02Spectral Ratios (pH 10, reduced with LDH): A
340/A
260: 0.43 ± 0.01
Moisture (KF): ≤ 8%Alcohol (EtOH): ≤ 0.05%Iron (Fe): ≤ 25 ppmStorage: -20°C, protect from moistureCAS #53-84-9
15339 1gm 5gm
β-Nicotinamide Adenine Dinucleotide, Lithium Salt, Dihydrate(β-NAD)
C21
H26
N7O
14P
2Li•2H
2O
Formula Weight: 705.39 (dihydrate) 663.43 (free acid)Free of LDH. Stable.Storage: -20°C
19438 250mg 1gm 5gm
-Nicotinamide Adenine Dinucleotide, Monosodium Salt(β-NAD)
C21
H26
N7O
14P
2Na•2H
2O
Formula Weight: 721.44 (dihydrate) 685.41 (anhydrous monosodium)PRODUCT SPECIFICATIONSForm: White to pale yellow powderAssay (Enzymatic): ≥ 95%Assay (UV 260 nm, pH 7.5): 100 ± 3%Spectral Ratios (pH 7.5): A
250/A
260: 0.83 ± 0.03
A280
/A260
: 0.21 ± 0.02Spectral Ratios (pH 10): A
340/A
260: 0.43 ± 0.01
A260
/A340
: 2.32 ± 0.06Moisture: ≤ 8%pH (0.4%, H
2O): 5.35 - 6.35
Sodium (Na): 3.0% ± 0.5%Storage: -20°CCAS #20111-18-6
19439 1gm 5gm
β-Nicotinamide Adenine Dinucleotide-Reduced, Disodium, Trihydrate(β-NADH; Coenzyme I-Reduced; Triphosphopyridine Nucleotide-Reduced; DPNH)
C21
H27
N7O
14P
2Na
2•3H
2O
Formula Weight: 763.45 (disodium, trihydrate) 709.40 (anhydrous, disodium)PRODUCT SPECIFICATIONSForm: White to cream colored powderAssay (Enzymatic): 99 ± 4%Assay (UV, 260 nm): 99 ± 4%ε
260 (10mM Tris): [14.4 ± 0.5] x 103
ε340
(10mM Tris): [6.3 ± 0.2] x 103
Spectral Ratios (10mM Tris): A
250/A
260 (red): 0.82 ± 0.03
A280
/A260
(red): 0.23 ± 0.02 A
340/A
260 (red): 0.43 ± 0.01
Moisture (KF): ≤ 8%Sodium (Na): 6.5 ± 1.5%Note: Do not use unbuffered water for dissolution
since it may cause decomposition of β-NADH.CAS #606-68-8
15345 100mg 250mg 500mg 1gm 5gm 10gm 25gm
Biochemicals
Biochemicals
99w w w . u s b w e b . c o m
-Nicotinamide Adenine Dinucleotide Phosphate, Monosodium, Tetrahydrate(β-NADP; Coenzyme II-Oxidized; Triphosphopyridine Nucleotide-Oxidized; TPN)
C21
H27
N7O
17P
3Na•4H
2O
Formula Weight: 837.45 (monosodium tetrahydrate) 765.39 (anhydrous, NADP•Na)PRODUCT SPECIFICATIONSAssay (Enzymatic): ≥ 93%Spectral Ratios (pH 7.5): A
250/A
260: 0.83 ± 0.03
A280
/A260
: 0.21 ± 0.02Spectral Ratios (pH 7.5, reduced with G6PDH): A
340/A
260: 0.43 ± 0.02
Moisture: ≤ 8.0%Sodium (Na): 3.0 ± 1.5%Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°C, protect from moisture.CAS #1184-16-3
22655 50mg 100mg 250mg 500mg 1gm 5gm
β-Nicotinamide Adenine Dinucleotide Phosphate-Reduced, Tetrasodium, Tetrahydrate(β-NADPH; Coenzyme II-Reduced; Triphosphopyridine Nucleotide-Reduced; TPNH)
C21
H26
N7O
17P
3•Na
4•4H
2O
Formula Weight: 905.41 (tetrasodium, tetrahydrate) 833.35 (anhydrous, tetrasodium)PRODUCT SPECIFICATIONSForm: White to slightly off-white powderAssay (Enzymatic): ≥ 93%Assay (UV, 260 nm): 100 ± 5%Assay (UV, 340 nm): 100 ± 5%Spectral Ratios (10mM Tris): A
340/A
260: 0.43 ± 0.01
Moisture: ≤ 8.0%Sodium (Na): 10.0 ± 2.0%Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°C, protect from moisture.CAS #2646-71-1
22650 25mg 50mg 100mg 250mg 500mg 1gm
Nicotinic AcidUSBioAnalyzedC
6H
5NO
2
Formula Weight: 123.11PRODUCT SPECIFICATIONSForm: White powderAssay: 99.0 - 101.0% (dry basis)Identity (A, B): Passes testsLoss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.02%Sulfate (SO
4): ≤ 0.02%
Heavy Metals (Pb): ≤ 0.002%Ordinary Impurities: Meets requirementsResidual Solvents: Meets requirementsCAS #59-67-6
19500 500gm 1kg 5kg
Ninhydrin, Monohydrate(1,2,3-Indantrione Hydrate; Triketohydrindene Hydrate)
ACS Reagent GradeC
9H
6O
4
Formula Weight: 178.14PRODUCT SPECIFICATIONSForm: White to brownish white crystalsIdentity and Melting Point: Passes testSolubility: Passes testSensitivity to Amino Acids: Passes testCAS #485-47-2
19515 10gm 25gm 50gm 100gm 500gm 1kg
p-Nitro Blue Tetrazolium Chloride(NBT)
ULTRAPUREC
40H
30Cl
2N
10O
6
Formula Weight: 817.64PRODUCT SPECIFICATIONSForm: Yellow crystalline powderIdentity: By IRSolubility (0.5% in H
2O): Passes test
Solubility (1% in MeOH): Passes testSensitivity: Passes testApplication:•Used with 5-bromo-4-chloro-3-indolyl
phosphate (BCIP) to detect antigen-antibody alkaline phosphatase complexes. The NBT/BCIP mix is converted to a dense blue compound and will develop at the site of antigen-antibody complexes.
NBT Stock Solution: 50 mg/ml in 70% dimethylformamideBCIP/NBT Developing Solution: 33 ml NBT (50 mg/ml) 5 ml AP Buffer (100mM Tris-HCl, pH 9.5,
100mM NaCl, 5mM MgCl2)
Add 16.8 ml BCIP stock (50 mg/ml in 100% dimethylformamide)
Protect from light and use within 1 hr.CAS #298-83-9
19535 250mg 500mg 1gm 5gm 10gm
4-Nitrophenyl-N-Acetyl-β-D-Galactosaminide(p-Nitrophenyl-2-Acetamido-2-Deoxy-β-D-Galactoside)
C14
H18
N2O
8
Formula Weight: 342.30Storage: -20°CCAS #14948-96-0
34128 100mg 1gm
2-Nitrophenyl-N-Acetyl-β-D-Glucosaminide(o-Nitrophenyl-2-Acetamido-2-Deoxy-β-D-Glucoside)
C14
H18
N2O
8
Formula Weight: 342.30Storage: -20°CCAS #13264-92-1
34129
Bioc
hem
ical
sBiochemicals
100 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
4-Nitrophenyl-N-Acetyl- -D-Glucosaminide(p-Nitrophenyl-2-Acetamido-2-Deoxy-α-D-Glucoside)
C14
H18
N2O
8
Formula Weight: 342.30Storage: -20°CCAS #10139-02-3
33248 100mg
4-Nitrophenyl-N-Acetyl-β-D-Glucosaminide(p-Nitrophenyl-2-Acetamido-2-Deoxy-β-D-Glucopyranoside)
C14
H18
N2O
8
Formula Weight: 342.30Storage: -20°CCAS #3459-18-5
34130 250mg 1gm
4-Nitrophenyl-β-D-Cellobioside(p-Nitrophenyl-β-D-Cellobioside)
C18
H25
NO13
Formula Weight: 463.39Storage: -20°CCAS #3482-57-3
33239 100mg 500mg
4-Nitrophenyl-β-D-Fucopyranoside(p-Nitrophenyl-β-D-Fucoside)
C12
H15
NO7
Formula Weight: 285.25Storage: -20°CCAS #1226-39-7
33240 100mg 1gm
4-Nitrophenyl-α-L-FucopyranosideC
12H
15NO
7
Formula Weight: 285.25PRODUCT SPECIFICATIONSForm: White powderIdentity: By IRMelting Point: 196 ± 2°CStorage: 4°C
34131 50mg 250mg 1gm
4-Nitrophenyl- -L-Fucopyranoside(p-Nitrophenyl-β-L-Fucoside)
C12
H15
NO7
Formula Weight: 285.25Storage: -20°CCAS #22153-71-5
34132 100mg 500mg
2-Nitrophenyl-β-D-GalactopyranosideSubstrate for β-GalactosidaseC
12H
15NO
8
Formula Weight: 301.25PRODUCT SPECIFICATIONSForm: White crystalline powderChromatography (TLC): HomogeneousIdentity: By IR[α]20
D : -66° ± 7° (c=1 in H
2O)
Storage: -20°C, desiccatedCAS #369-07-3
19593 500mg 1gm 5gm 25gm
4-Nitrophenyl-α-D-Galactopyranoside(p-Nitrophenyl-α-D-Galactoside)
C12
H15
NO8
Formula Weight: 301.25Storage: -20°CCAS #7493-95-0
34133 250mg 500mg 1gm 5gm
4-Nitrophenyl-β-D-Galactopyranoside(PNPG)
C12
H15
NO8
Formula Weight: 301.25PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderIdentity: By IR[α]20
D : -83° ± 3° (dry basis, c=1 in H
2O)
A (425 nm, 8 mg/ml, 1 cm): ≤ 0.07 (in 0.15M AMP buffer, pH 10.25)
Moisture (KF): ≤ 10%Free p-Nitrophenol: ≤ 0.01%Storage: -20°C, protect from moisture.CAS #3150-24-1
34134 1gm 5gm
2-Nitrophenyl- -D-Glucopyranoside(o-Nitrophenyl-β-D-Glucoside)
C12
H15
NO8
Formula Weight: 301.25Storage: -20°CCAS #2816-24-2
33250 250mg 1gm 5gm
4-Nitrophenyl-α-D-GlucopyranosideC
12H
15NO
8
Formula Weight: 301.25Storage: -20°CCAS #3767-28-0
34135 1gm 5gm
4-Nitrophenyl-β-D-GlucopyranosideC
12H
15NO
8
Formula Weight: 301.25PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : -106° ± 5° (dry basis, c=1 in H
2O)
A (425 nm, 8 mg/ml, 1 cm): ≤ 0.07 (in 0.15M AMP buffer, pH 10.25)
Moisture (KF): ≤ 10%Free p-Nitrophenol: ≤ 0.01%Storage: -20°C, protect from moisture.CAS #2492-87-7
19594 250mg 500mg 1gm 5gm
4-Nitrophenyl-β-D-GlucuronideC
12H
13NO
9
Formula Weight: 315.23Storage: 4°CCAS #10344-94-2
34136 100mg 250mg 1gm
Biochemicals
Biochemicals
101w w w . u s b w e b . c o m
4-Nitrophenyl- -D-Lactopyranoside(p-Nitrophenyl-β-D-Lactoside)
C18
H25
NO13
Formula Weight: 463.39Storage: -20°CCAS #4419-94-7
34137 100mg 500mg
4-Nitrophenyl-α-D-MaltosideC
18H
25NO
13
Formula Weight: 463.39Storage: 4°CCAS #17400-77-0
34138 10mg 50mg 100mg
4-Nitrophenyl-α-D-Mannopyranoside(p-Nitrophenyl-α-D-Mannoside)
C12
H15
NO8
Formula Weight: 301.25Storage: -20°CCAS #10357-27-4
34139 250mg 1gm 5gm
4-Nitrophenyl-β-D-Mannopyranoside(p-Nitrophenyl-β-D-Mannoside)
C12
H15
NO8
Formula Weight: 301.25Storage: -20°CCAS #35599-02-1
34140 100mg 500mg
4-Nitrophenyl Phosphate, Dicyclohexylammonium Salt
NO2C
6H
4OPO(OH)
2(C
6H
11NH
2)2•H
2O
Formula Weight: 435.45This is a particularly stable salt form of
p-Nitrophenyl Phosphate.Storage: 4°CCAS #14545-82-5
19600 5gm 25gm 100gm
4-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate
C6H
4NO
6PNa
2•6H
2O
Formula Weight: 371.14PRODUCT SPECIFICATIONSForm: Light yellow crystalline powderMoisture: ≤ 32%p-Nitrophenyl: ≤ 0.005%Free Inorganic Phosphate: ≤ 1%Storage: 4°CCAS #4264-83-9
19587 1gm 10gm 25gm 50gm
4-Nitrophenyl Phosphate, Ditris SaltC
14H
28N
3O
12P
Formula Weight: 461.36PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderIdentity: By IRSpectral Ratios (425 nm, 8 mg/ml, 1 cm): ≤ 0.070
λmax
: 309 ± 1 nmFree p-nitrophenol: ≤ 0.010%Moisture (KF): ≤ 1.5%Storage: 4°CCAS #68189-42-4
19603 1gm 5gm 25gm 100gm
4-Nitrophenyl ThioacetateC
8H
7NO
3S
Formula Weight: 197.27Storage: 4°C
19605 5gm 25gm
2-Nitrophenyl-β-D-Thio-Galactopyranoside
C12
H15
NO7S
Formula Weight: 317.32Storage: -20°CCAS #1158-17-4
19599 25mg 100mg 500mg
4-Nitrophenyl- -D-XylopyranosideC
11H
13NO
7
Formula Weight: 271.22Storage: -20°C
34142 100mg 500mg 1gm
Nonidet P40 SubstituteIgepal CA-630, ([Octylphenoxy]polyethoxyethanol; NP-40)
ULTRAPUREPRODUCT SPECIFICATIONSForm: Clear, slightly yellow-green viscous liquid(n20
d) : 1.06 ± 0.005
Chemically indistinguishable from Nonidet P40, which is no longer commercially available.
19628 500ml 1L
Norit®
See Charcoal-Activated Norit, page 205
DL-NorleucineCH
3(CH
2)3CH(NH
2)COOH
Formula Weight: 131.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IRChromatography (TLC): HomogeneousMoisture: ≤ 1.0%CAS #616-06-8
19635 25gm 100gm 1kg
DL-Norvaline(DL-2-Aminopentanoic Acid)
CH3(CH
2)2CH(NH
2)COOH
Formula Weight: 117.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRCAS #760-78-1
19660 25gm 100gm
NP-40See Nonidet P40 Substitute, page 265
Bioc
hem
ical
sBiochemicals
10� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Nucleoside-5'-Triphosphates (ATP, CTP, GTP, UTP), Set of Four, 100mM Solutions(NTPs)
ULTRAPURE100mM aqueous solutions, pH 7.5Storage: -20°C
4x25µmol(250µl)eachNTPperpack.77245 1pk
Nucleotide SolutionsSee Adenosine-5'-Triphosphate (ATP), page 174See Cytidine-5'-Triphosphate (CTP), page 213See 2'-Deoxyadenosine-5'-Triphosphate (dATP),
page 214See 2'-Deoxycytidine-5'-Triphosphate (dCTP),
page 215See 2'-Deoxyguanosine-5'-Triphosphate (dGTP),
page 216See 2'-Deoxyinosine-5'-Triphosphate (dITP),
page 217See 2'-Deoxynucleoside-5'-Triphosphates
(dNTP), Set of Four, page 217See 2'-Deoxythymidine-5'-Triphosphate (dTTP),
page 217See 2'-Deoxyuridine-5'-Triphosphate (dUTP),
page 218See 2',3'-Dideoxyadenosine-5'-Triphosphate
(ddATP), page 220See 2',3'-Dideoxycytidine-5'-Triphosphate
(ddCTP), page 220See 2',3'-Dideoxyguanosine-5'-Triphosphate
(ddGTP), page 220See 2',3'-Dideoxythymidine-5'-Triphosphate
(ddTTP), page 220See Guanosine-5'-Triphosphate (GTP), page 238See PCR-Nucleotide Mixes, page 268See Uridine-5'-Triphosphate (UTP), page 306
Nystatin(Mycostatin)
USBioAnalyzedPRODUCT SPECIFICATIONSForm: Yellow to light tan powderActivity: ≥ 4,400 USP Nystatin units/mg (as is) ≥ 4,631 USP Nystatin units/mg (dry basis)Identity: By UV, A
230/A
279 = 0.90 - 1.25
pH (3%, H2O): 6.0 - 8.0
Loss on Drying: ≤ 5.0%Storage: -20°C in tightly closed light resistant
containersCAS #1400-61-9
19770 1mu 5mu 10mu
NZCYM Broth, Ready-Made PowderForm: Off-white free flowing powderpH (2.2%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 10 gm/L Casamino Acids: 1 gm/L Magnesium Sulfate: 0.98 gm/L Yeast Extract: 5 gm/L Sodium Chloride: 5 gm/LTo use: Dissolve 22 gm powder per liter of
purified water. Autoclave.Application: •For the propagation and maintenance of lambda
bacteriophage
75855 250gm 1kg
n-Octadecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate
See Sulfobetaine 18 (SB3-18), page 293
n-Octyl-β-D-Glucopyranoside
ULTRAPUREC
14H
28O
6
Formula Weight: 292.37Storage: -20°C, protect from moisture.CAS #29836-26-8
19775 1gm 5gm 10gm
n-Octyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate
See Sulfobetaine 8 (SB3-8), page 292
n-Octyl-β-D-ThioglucopyranosideC
14H
28O
5S
Formula Weight: 308.43PRODUCT SPECIFICATIONSForm: White powderAssay (HPLC): ≥ 99.0%Identity: By IRSolubility (50 mg/ml in Ethanol): Clear, colorless
solutionCarbon: 54.0 - 55.0% (dry basis)Storage: -20°C
19669 1gm 5gm
Oleic Acid(9-Octadecanoic Acid)
CH3(CH
2)7CH=CH(CH
2)7COOH
Formula Weight: 282.46PRODUCT SPECIFICATIONSForm: Clear, colorless oilAssay: ≥ 99.0% (by GLC)Storage: 4°C, vacuum sealed ampules or under
inert gas.CAS #112-80-1
19785 1gm 5gm 25gm
Oligo (dT)12-18 Primer, MN ≅ 4500 MB GradeForm: 25µM in dH
2O
2.5 nmol per tube.Storage: -20°C
77405 100µl(2.5nmol)
Oligo p(dT) Cellulose(Oligo dT Cellulose)
ULTRAPUREBinding Capacity: 40 - 100 A
260 units/gm of
celluloseAssayed for quantitative poly A and 3H mRNA
(HeLa cells) bindingStorage: -20°C
71547 1gm
Oligo p(dT)12-18 Sodium Salt(Oligo dT)
LyophilizedForm: This oligodeoxythymidylate mixture
is synthesized using O-cyanoethyl phosphoramidite chemistry. After the removal of the protecting groups, the product is converted to the sodium salt and the excess salt removed by gel filtration.
Storage: -20°C
19817 5units 25units 100units
Oligomycin APRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 90%Storage: -20°CCAS #579-13-5
19803 10mg 50mg
Biochemicals
Biochemicals
103w w w . u s b w e b . c o m
Olive OilReagent GradeCAS #8001-25-0
19805 1lb 25lb
ONPGSee 2-Nitrophenyl-β-D-Galactopyranoside,
page 264
Orcinol, Monohydrate(5-Methylresorcinol)
C7H
8O
2•H
2O
Formula Weight: 142.15PRODUCT SPECIFICATIONSForm: White to beige crystalline powderAssay: ≥ 99.0% (dry basis)Chromatography (TLC): HomogeneousIdentity: By IRMelting Point: 56 - 61°CMoisture (KF): ≤ 13.7%CAS #6153-39-5
19815 5gm 10gm 25gm 100gm
D-Ornithine, HydrochlorideNH
2(CH
2)3CH(NH
2)COOH•HCl
Formula Weight: 168.62CAS #16682-12-5
19820 1gm 5gm 10gm
DL-Ornithine, HydrochlorideNH
2(CH
2)3CH(NH
2)COOH•HCl
Formula Weight: 168.62PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRChromatography (TLC): HomogeneousMelting Point: 230 - 240°CLoss on Drying: ≤ 1.5%CAS #1069-31-4
19825 10gm 25gm 100gm
L-Ornithine, HydrochlorideNH
2(CH
2)3CH(NH
2)COOH•HCl
Formula Weight: 168.62PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IR[α]20
D : +23.0° to +25.0° (c=4 in 6N HCl)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 20 ppmAmmonia (NH
4): ≤ 0.02%
Arsenic (As): ≤ 1 ppmIron (Fe): ≤ 20 ppmSulfate (SO
4): ≤ 0.03%
CAS #3184-13-2
19830 25gm 100gm 1kg 5kg
Ouabain(Strophanthin-G)
C29
H44
O12
•8H2O
Formula Weight: 728.77CAS #11018-89-6
19833 250mg 1gm 5gm
OvalbuminSee Albumin, Egg, page 188
Oxalacetic Acid(Hydroxymaleic Acid)
C4H
4O
4
Formula Weight: 132.07PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0% Identity: By IRStorage: -20°CCAS #328-42-7
19837 1gm 5gm 10gm 25gm 100gm
Oxamic Acid, Free AcidNH
2COCOOH
Formula Weight: 89.05CAS #471-47-6
19843 5gm 10gm 25gm 100gm
Oxamic Acid, Sodium Salt(Sodium Oxamate)
NH2COCOONa
Formula Weight: 111.03PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0%Identity: By IRLoss on Drying: ≤ 1.0%pH (1%, H
2O): 5.4 - 6.5
Solubility (2%, H2O): Clear, colorless
CAS #595-73-1
19829 5gm 25gm 100gm
Ox Bile Extract, Bile Salts
19845 100gm
Oxytetracycline, HydrochlorideC
22H
24N
2O
9•HCl
Formula Weight: 496.89PRODUCT SPECIFICATIONSForm: Light yellow crystalline powderActivity: ≥ 835 µg of C
22H
24N
2O
9/mg (dry basis)
≥ 818 µg of C22
H24
N2O
9/mg (as is)
Identity: By UVpH (10 mg/ml): 2.0 - 3.0Loss on Drying: ≤ 2.0%Storage: Store protected from light.CAS #2058-46-0
23659 50gm 100gm
PABASee p-Amino Benzoic Acid, page 189
Bioc
hem
ical
sBiochemicals
10� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Pancreatin 1XSource: Porcine PancreasForm: Powder stabilized with lactose.Unit Definition: Pancreatin will convert at least 25
times its weight of potato starch into soluble carbohydrates in 5 min at 40°C and at least 25 times its weight of casein into proteases in
1 hr at 40°C.
19880 200gm 1kg
Pancreatin 5XSource: Porcine PancreasPRODUCT SPECIFICATIONSForm: Off-white to light tan powderIdentity: By IRProtease: ≥ 125 USP units/mgAmylase: ≥ 125 USP units/mgLipase: ≥ 10 USP units/mgE. coli : NegativeSalmonella: NegativeLoss on Drying: ≤ 6.8%
19890 50gm 100gm 500gm
Pantothenic Acid, Calcium SaltSee Calcium Pantothenate, page 202
D-Pantothenyl AlcoholC
9H
19NO
4
Formula Weight: 205.25CAS #81-13-0
19905 25gm
Papain(E.C.3.4.22.2)Source: Papaya LatexPRODUCT SPECIFICATIONSForm: Off-white powderActivity: Approx. 70 Milk Clotting Units/mgLoss on Drying: ≤ 7.0%CAS #9001-73-4
19930 100gm 500gm 1kg
Papain(E.C.3.4.22.2)Source: Papaya LatexPRODUCT SPECIFICATIONSForm: 2X crystallized, suspension in 0.05 NaOAc
(pH 4.5) with 0.02% sodium azide. Prepared by method of Kimmel and Smith, (1954) J. Biol. Chem. 207, 515.
Activity: ≥ 15 units/mg proteinUnit Definition: One unit hydrolyzes 1 µmol of
BAEE (PN 11960)/min at 25°C, pH 6.2.Storage: 4°C
19925 100mg 500mg 1gm
Paraformaldehyde Solution, 4% in PBSDescription: Paraformaldehyde solution 4% in
phosphate buffered salinePRODUCT SPECIFICATIONSForm: Colorless, clear solutionPurity (Formaldehyde): 91 - 93%pH (25°C): 7.0 - 7.6Storage: 4°C
19943 1L
PararosanilineSee Basic Fuchsin, page 196
PBS, 10X Solution, pH 7.4
ULTRAPUREMB GradeForm: 10X PBS (Phosphate Buffered Saline)
solution consisting of 80.6mM sodium phosphate, 19.4mM potassium phosphate, 27mM KCl and 1.37M NaCl in high purity dH
2O, pH 7.4. Solution is 0.2 µm filtered
and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity dH
2O for a solution
consisting of 10mM phosphate, pH 7.4 ± 1 2.7mM potassium chloride and 137mM NaCl.
pH (25°C, 10X): 7.4 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
75889 100ml 1L 5L
PCR Nucleotide Mix, 10mM Solution
ULTRAPUREForm: Premixed dNTPs at 10mM each dATP,
dCTP, dGTP, dTTP.Functionally tested in PCR with USB Taq DNA
Polymerase (PN 71160).
77212 500µl
PCR Nucleotide Mix, 25mM Solution
ULTRAPUREForm: Premixed dNTPs at 25mM each dATP,
dCTP, dGTP, dTTP.Functionally tested in PCR with USB Taq DNA
Polymerase (PN 71160).
77119 500µl
PCR Nucleotide Mix with dUTP, 10mM Solution
ULTRAPUREForm: Premixed dNTPs at 10mM each dATP,
dCTP, dGTP, dUTPFunctionally tested in PCR with USB Taq DNA
Polymerase (PN 71160).
77330 500µl
PDT Disulfonate, Disodium SaltC
20H
12N
4O
6S
2Na
2
Formula Weight: 514.44 (anhydrous) 550.47 (dihydrate)PRODUCT SPECIFICATIONSForm: Yellow powderAssay (UV): ≥ 95.0% (Fe complex, pH 4.75) as is,
dihydrateIdentity: By IRλ
max: 562 ± 2 nm
εmax
: ≥ 26.5 x 103
Moisture (KF): ≤ 10%Note: The assay value is calculated using
MW=1707.26 for the Fe (PDT•Na2•2H
2O)
3
complex and theoretical εmax
=27.9 x 103.CAS #28048-33-1
20061 1gm 5gm 25gm
Pectin(Polygalacturonic Acid Methyl Ester)
Source: Citrus FruitsCAS #9000-69-5
19955 100gm 500gm
Biochemicals
Biochemicals
105w w w . u s b w e b . c o m
Pectinase(E.C.3.2.1.15)Source: Aspergillus nigerPRODUCT SPECIFICATIONSForm: Beige powderActivity: ≥ 1.0 units/mgUnit Definition: One unit releases 1 µmol of
galacturonic acid from polygalacturonic acid per minute at 25°C, pH 4.0.
Storage: 4°CCAS #9032-75-1
19962 50gm 100gm 500gm
PEGSee Polyethylene Glycol 400, page 274See Polyethylene Glycol 6000, page 274See Polyethylene Glycol 8000, page 274
Pelargonic Acid(Nonanoic Acid)
C9H
18O
2
Formula Weight: 158.24PRODUCT SPECIFICATIONSForm: Clear liquidAssay (GC): ≥ 99%CAS #112-05-0
19970 1gm 5gm
Penicillin G, PotassiumUSBioAnalyzedC
16H
17KN
2O
4S
Formula Weight: 372.48PRODUCT SPECIFICATIONSForm: White powderAssay: 1440 - 1680 Penicillin G units/mg (as is)Identity A: By IRIdentity B: Passes testpH (60 mg/ml): 5.0 - 7.5Loss on Drying: ≤ 1.5%Crystallinity: Passes testMode of Action:•Interferes with bacterial cell wall synthesis and
cell division.•Inactivated by oxidizing and reducing agents,
glycerols and alcohols.CAS #113-98-4
19985 100mu 1kg
Penicillin G, Sodium
ULTRAPUREUSBioAnalyzedC
16H
17N
2NaO
4S
Formula Weight: 356.37PRODUCT SPECIFICATIONSForm: White powderActivity: 1500 - 1750 Penicillin G units/mg (as is)Crystallinity: Passes testIdentity (A, B): Passes testspH (60 mg/ml): 5.0 - 7.5Loss on Drying: ≤ 1.5%Mode of Action:•Interferes with bacterial cell wall synthesis and
cell division.•Inactivated by oxidizing and reducing agents,
glycerols and alcohols.CAS #69-57-8
19995 100mu 1kg
Pentaethyleneglycol MonooctyletherC
18H
38O
6
Formula Weight: 350.49
15722
PEPSee Phosphoenol Pyruvic Acid, page 272
Pepsin(E.C.3.4.23.1)Source: Porcine StomachPRODUCT SPECIFICATIONSForm: Lyophilized, salt free, 2X crystallizedActivity: ≥ 2500 units/mgUnit Definition: One unit causes an increase in
absorbance at 280 nm of 0.001/min at 37°C, pH 2.0.
Storage: -20°CCAS #9001-75-6
20010 1gm 5gm 10gm 25gm
Pepsin 1:10,000(E.C.3.4.23.1)Source: Porcine StomachPRODUCT SPECIFICATIONSForm: Stabilized powderActivity: 1 gm will digest approximately
10,000 gm of egg albumin under standard assay conditions.
20015 50gm 100gm 500gm 1kg
Pepstatin A(Isovaleryl-Val-Val-4-Amino-3-Hydroxy-6-Methylheptanoyl-Ala-4-Amino-3-Hydroxy-6-Methylheptanoic Acid)
ULTRAPUREC
34H
63N
5O
9
Formula Weight: 685.89PRODUCT SPECIFICATIONSForm: White powderChromatography (TLC): HomogeneousPeptide Content: ≥ 98%Biological Activity: ≥ 850 µg/mgApplication:•Pepstatin A, a protease inhibitor, inhibits
Pepsin, Renin and Cathepsin D. Effective concentrations are 10-6 to 10-9M. It does not inhibit Trypsin, Chymotrypsin, Papain or Plasmin.
Note: Dissolves in methanol.Storage: 4°CCAS #26305-03-6
20037 5mg 25mg
Peptic Peptone B(Peptones)
PRODUCT SPECIFICATIONSForm: Peptic hydrolysate of beef tissueNitrogen: 10.5 - 12.5%Moisture: ≤ 5.0%pH (10%, H
2O): 7.0 ± 0.3
Ash: 12.5 - 16.5%Salmonella: NegativeAn excellent media for bacteriological work with
many aerobic and anaerobic bacteria.
20040 4oz 1lb 5lb 25lb
Bioc
hem
ical
sBiochemicals
106 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
PeptonesSee Bacteriological Peptone, page 196See Casein Hydrolysate, page 203See Lactalbumin Hydrolysate, page 245See Peptic Peptone, page 269See Soy Hydrolysate, page 289See Yeast Hydrolysate and Extract, page 310
Peroxidase(E.C.1.11.1.7)Source: HorseradishPRODUCT SPECIFICATIONSForm: Lyophilized, salt free, chromatographically
pureActivity: RZ greater than 3.0; 250 guaiacol units/
mg (3500 IUB units/mg).Unit Definition: One unit decomposes 1 µmol
of substrate/min at 25°C, pH 6.0. One guaiacol unit at 25°C is approx. equal to one purpurogallin unit at 20°C.
Storage: -20°CCAS #9003-99-0
20055 25mg 100mg 500mg
Peroxidase(E.C.1.11.1.7)Source: HorseradishPRODUCT SPECIFICATIONSForm: Lyophilized, salt freeActivity: RZ approx. 0.6; 60 purpurogallin
units/mg (800 IUB units/mg).Unit Definition: One unit produces 1 mg of
purpurogallin in 20 sec. at 20°C.Storage: -20°C
20057 250mg 500mg 1gm
Peroxidase(E.C.1.11.1.7)Source: HorseradishPRODUCT SPECIFICATIONSForm: Lyophilized, salt freeActivity: RZ: ≥ 1.0; 100 purpurogallin units/mg
(1250 IUB units/mg)Unit Definition: One unit produces 1 mg of
purpurogallin in 20 sec. at 20°C.Storage: -20°CCAS #9003-99-0
20058 50,000units 125,000units 500,000units
Peroxidase ConjugateSee Canavalia ensiformas Agglutinin-Peroxidase
Conjugate, page 202
r-hPGM-B, r-hPGM-MSee Phosphoglycerate Mutase-B, -M, page 273
Phaseolus Vulgaris Agglutinin-Pure(Phytohemagglutinin with Mucoprotein)
Source: Red Kidney BeanForm: LyophilizedStorage: -20°C
34490 10mg
Phenol(Carbolic Acid)
ULTRAPUREMB GradeC
6H
5OH
Formula Weight: 94.11PRODUCT SPECIFICATIONSForm: Translucent crystalline solidAssay: ≥ 99%Clarity of Solution (5%, H
2O): Passes test
pH (5%, H2O): 4.5 - 6.0
Freezing Point: ≥ 40.5°CWater: ≤ 0.1%Res. on Evaporation: ≤ 0.05%Buffer Equilibration Suitability: Passes testIron (Fe): ≤ 1 ppmLead (Pb): ≤ 2 ppmMagnesium (Mg): ≤ 1 ppmDNase (endo): Passes testPreservatives/Inhibitors: Passes testApplication:•Used primarily for DNA/RNA extraction and
purificationPreparation of buffered phenol:1. Allow phenol to warm to room temperature and
then melt at 68°C.2. Add an equal volume of buffer (0.5M Tris-HCl,
pH 8.0).3. Stir for about 15 minutes on a magnetic stirrer.4. When the two phases have separated, aspirate
the top (aqueous) phase.5. Add an equal volume of 0.1M Tris-HCl, pH 8.0
and repeat stirring.6. Remove the aqueous phase and repeat
extractions until the pH of the phenol is 7.8. (Note: Measure with pH paper).
7. After the phenol is equilibrated, remove the final aqueous phase. Top with 0.1 volumes of 0.1M Tris-HCl, pH 8.0.
8. Store in a light-tight bottle at 4°C.Note: For convenience, USB provides phenol
which is already equilibrated (Product Code 75829 or 77510).
Storage: -20°CPacked under inert atmosphereCAS #108-95-2
75830 100gm 500gm
Biochemicals
Biochemicals
107w w w . u s b w e b . c o m
Phenol, pH 4.5, Equilibrated
ULTRAPUREMB GradeForm: Liquid phenol equilibrated with citrate
bufferpH (5%, H
2O solution of phenol layer, 20°C): 4.5
± 0.1 (by pH meter). Topped with citrate buffer and packed under inert atmosphere.
Contaminant Testing: DNase: Passes testStorage: 4°CApplication: •More suited for RNA purification procedures
77510 100ml 400ml
Phenol, pH 8.0, Equilibrated
ULTRAPUREMB GradeForm: This product is supplied as water-saturated
phenol with a separate bottle of Phenol Equilibration Buffer (PN 75833). At the time of product use, simply add the entire contents of Phenol Equilibration Buffer to the water-saturated phenol.
pH (5%, after equilibration with PN 75833, 20°C): ≥ 8.0
Contaminant Testing: DNase: Passes testStorage: 4°CApplication: •Used in nucleic acid purification procedures and
dissolution of protein
75829 100ml 400ml
Phenol: Chloroform: Isoamyl Alcohol (25:24:1)
ULTRAPUREMB GradeForm: A liquid mixture of equilibrated phenol, ACS
Reagent Grade chloroform and ACS Reagent Grade isoamyl alcohol in a ratio of 25:24:1.
Contaminant Testing: DNase: Passes testApplication: •Nucleic acid extraction and purificationPhenol/Chloroform RNA Isolation MethodA. Guanidine HCl Homogenization Buffer for RNA
Isolation: 8.0M Guanidine HCl, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate.
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25 ml of phenol is required for every 10 ml of sample.
Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Product Code 75829 or 77510).
C. RNA Resuspension Buffer: TE, pH 7.5.Storage: 4°C
75831 100ml 400ml
PhenolphthaleinACS Reagent GradeC
20H
14O
4
Formula Weight: 318.32PRODUCT SPECIFICATIONSForm: White to off-white powderClarity of Alcohol Solution (1%, EtOH): Passes testVisual Transition: From pH 8.0: Colorless to pH 10.0: RedCAS #77-09-8
20075 250gm 500gm 1kg
Phenolphthalein Diphosphate, Tetrasodium Salt
C20
H12
Na4O
10P
2•nH
2O
Formula Weight: 566.21•nH2O
PRODUCT SPECIFICATIONSForm: Pale beige powderAssay: ≥ 90% (dry basis)Identity: By IRMoisture (KF): ≤ 20%CAS #6880-90-9
20074 5gm 10gm
Phenol Red, Free Acid(Phenolsulfonphthalein)
ACS Reagent GradeC
19H
14O
5S
Formula Weight: 354.38PRODUCT SPECIFICATIONSForm: Rust/red crystalline powderClarity of Solution: Passes testVisual Transition Interval: pH 6.8 - Yellow pH 8.2 - RedCAS #143-74-8
20086 10gm 25gm 50gm
Phenol Red, Sodium Salt(Phenolsulfonphthalein)
ACS Reagent GradeC
19H
13O
5SNa
Formula Weight: 376.36PRODUCT SPECIFICATIONSForm: Rust/red crystalline powderClarity of Solution: Passes testVisual Transition Interval: pH 6.8 - Yellow pH 8.2 - RedCAS #34487-61-1
20085 5gm 10gm 25gm 50gm
PhenolsulfonphthaleinSee Phenol Red, page 271
Bioc
hem
ical
sBiochemicals
108 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
DL-PhenylalanineC
6H
5CH
2CH(NH
2)COOH
Formula Weight: 165.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Chromatagraphy (TLC): HomogeneousIdentity: By IRLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.3%Heavy Metals (Pb): ≤ 10 ppmAmmonia (NH
3): ≤ 0.03%
Arsenic (As): ≤ 3 ppmChloride (Cl): ≤ 0.02%Iron (Fe): ≤ 50 ppmSulfate (SO
4): ≤ 0.04%
CAS #150-30-1
20100 25gm 100gm 1kg
L-PhenylalanineUSBioAnalyzed(C
6H
5)CH
2CH(NH
2)COOH
Formula Weight: 165.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Chromatographic Purity: Passes testIdentity: By IRLoss on Drying: ≤ 0.3%[α]25
D : -32.7° to -34.7° (c=2 in H
2O)
pH (1%, H2O): 5.4 - 6.0
Residue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 0.0015%Arsenic (As): ≤ 1.5 ppmChloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%O.V.I.: Meets requirementsCAS #63-91-2
20105 10gm 25gm 100gm 250gm 1kg 5kg
Phenyl- -D-GlucopyranosideC
12H
16O
6•nH
2O
Formula Weight: 256.25PRODUCT SPECIFICATIONSForm: White powderIdentity: By IR[α]20
D : -71.5° to -73.5° (c=2 in H
2O, dry basis)
Moisture (KF): ≤ 12.5%CAS #1454-44-4
20185 5gm 25gm
DL-α-Phenyl GlycineC
8H
9NO
2
Formula Weight: 151.16CAS #2835-06-5
20120 10gm 25gm 100gm
Phenylmethyl Sulfonyl Fluoride(PMSF)
ULTRAPUREC
7H
7FO
2S
Formula Weight: 174.19PRODUCT SPECIFICATIONSForm: Off-white/clear crystalsAssay: ≥ 99.0%Identity: By IRApplication: •PMSF is a protease inhibitor active against
trypsin and chymotrypsinCAS #329-98-6
20203 1gm 5gm 25gm 100gm
Phloroglucinol(1,3,5-Trihydroxybenzene)
C6H
6O
3
Formula Weight: 120.13CAS #108-73-6
20220 25gm 100gm
PhosphocreatineSee Creatine Phosphate, page 210
Phosphoenol Pyruvic Acid, Monocyclohexylammonium Salt
C9H
18NO
6P
Formula Weight: 267.22Storage: -20°C
20250 100mg 1gm 5gm 25gm
Phosphoenol Pyruvic Acid, Trisodium Salt, Heptahydrate(PEP)
C3H
2O
6PNa
3•7H
2O
Formula Weight: 360.09 (heptahydrate)PRODUCT SPECIFICATIONSForm: White to slightly off-white powderAssay: ≥ 98.0%Assay (calc. from P organic): ≥ 45.0%Identity: By IRSolubility (5%, H
2O): Clear and colorless
Ptotal
: 8.3 - 8.9%P
inorg: ≤ 0.2%
Porganic
: ≥ 8.2%Moisture (KF): 35.0 ± 2.0%
20253 250mg 1gm 5gm
Phosphoenol Pyruvic Acid, Tricyclohexylammonium Salt, Monohydrate
[CH2=C(OPO
3)COO](C
6H
11NH
3)3•H
2O
Formula Weight: 483.58PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV): ≥ 98.0% (dry basis)Identity: By IRChromatography (TLC): HomogeneousSolubility (1%, H
2O): Clear and colorless
pH (1%, H2O): 7.5 - 8.5
Moisture (KF): ≤ 4.5%Storage: -20°CCAS #35556-70-8
20255 100mg 1gm 5gm 25gm
Biochemicals
Biochemicals
109w w w . u s b w e b . c o m
Phosphoglycerate Mutase - B, Recombinant(r-hPGM-B)
Source: Human Recombinant from E. coliPRODUCT SPECIFICATIONSForm: Solution in 10mM phosphate buffer
(pH 7.0)Concentration: Approx. 1 mg/mlSpecific Activity: ≥ 500 units/mg proteinPurity: Single band at about 29 kDa, as
determined by SDS-PAGEUnit Definition: One unit defines one µmole
of NAD+ production per minute at 30°C determined according to the method described by Bergmeyer, et. al.(1)
Reference:1. Bergmeyer, et. al., Method of Enzymatic
Analysis, 3rd Ed., (1983), 282-283.Storage: -20°C
26118 100µg
Phosphoglycerate Mutase - M, Recombinant(r-hPGM-M)
Source: Human Recombinant from E. coliPRODUCT SPECIFICATIONSForm: Solution in 50mM phosphate buffer,
pH 6.5, 1mM β-MEConcentration: Approx. 1 mg/mlSpecific Activity: ≥ 500 units/mg proteinPurity: Single band at about 29 kDa, as
determined by SDS-PAGEUnit Definition: One unit defines one µmole
of NAD+ production per minute at 30°C determined according to the method described by Bergmeyer, et. al.(1)
Reference:1. Bergmeyer, et. al., Method of Enzymatic
Analysis, 3rd Ed., (1983), 282-283.Storage: -20°C
26119 100µg
PhytohemagglutininSee Phaseolus Vulgaris Agglutinin, page 270
PIPES(Piperazine-N,N'-bis-[2-Ethanesulfonic Acid])
C8H
18N
2O
6S
2
Formula Weight: 302.37PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 6.80 ± 0.15A (250 nm, 5% in 1N NaOH, 1 cm): ≤ 0.20Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #5625-37-6
20426 25gm 100gm 1kg
PIPES, Dipotassium Salt(Piperazine-N,N'-bis-[2-Ethanesulfonic Acid], Dipotassium Salt)
C8H
16N
2O
6S
2K
2
Formula Weight: 378.55PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRA (260 nm, 5%, 1 cm): ≤ 0.1Moisture (KF): ≤ 10.0%
20429 10gm 25gm 100gm 1kg
PIPES, Disodium SaltC
8H
16N
2O
6S
2Na
2
Formula Weight: 346.33PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0% (dry basis)Identity: By IRpKa (20°C): 6.80 ± 0.15A (250 nm, 0.1M, 1 cm): ≤ 0.1Loss on Drying: ≤ 10.0%CAS #76836-02-7
20428 10gm 25gm 100gm 1kg
PIPES, Sesquisodium Salt, Monohydrate
C8H
16.5O
6N
2S
2•1.5Na•H
2O
Formula Weight: 353.36PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0% (dry basis)pKa (20°C): 6.80 ± 0.15Identity: By IRA (260 nm, 0.1M, 1 cm): ≤ 0.05pH (0.1M, H
2O): 6.5 - 6.9
Loss on Drying: ≤ 7.0%Chloride (Cl): ≤ 0.1%Sodium (Na): ≤ 11.0%Note: This product contains approximately 1.5
equivalents of sodium per molecule of PIPES as well as approximately 1 molecule of water of crystallization. Variation may be as much as ±10% from exactly 1.5 equivalents of sodium per mole.
CAS #100037-69-2
20427 25gm 100gm 1kg
PMSFSee Phenylmethyl Sulfonyl Fluoride, page 272
PNPGSee 4-Nitrophenyl-β-D-Galactopyranoside,
page 264
Polyadenylic Acid, Potassium SaltSee Molecular Biology Products & Kits Section,
page 92
Polyanetholesulfonic Acid, Sodium Salt
(C10
H11
NaO4S)
n
Formula Weight: (250.25)n
CAS #55963-78-5
20450 5gm 25gm 100gm
Bioc
hem
ical
sBiochemicals
110 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Polydeoxy (Inosinate-Cytidylate) Acid, Sodium Salt
PRODUCT SPECIFICATIONSDescription: This double-stranded polymer was
prepared by the action of highly purified DNA polymerase upon a mixture of deoxyinosine triphosphate and deoxycytidine triphosphate using a primer of strictly alternating dI and dC. After enzymatic synthesis the polymer was extracted with chloroform/isoamyl alcohol to remove protein, dialyzed against aqueous sodium chloride, then exhaustively dialyzed against deionized water to remove salt. The product was then lyophilized.
Form: Lyophilized powderAbsorbance per unit weight found:
13.0 - 20.8 A260
units/mgUV Absorption Spectrum: λ
max: 248 - 253 nm
λmin
: 224 - 229 nmSpectral Ratios: A
290/A
260: 0.30 - 0.41
A280
/A260
: 0.70 - 0.72 A
270/A
260: 0.82 - 0.87
A250
/A260
: 1.19 - 1.26All UV absorbance data were obtained in 0.02M
phosphate buffer, pH 7.0.Storage:-20°C
20539 5units 25units 100units
Polyethylene Glycol 400(PEG 400)
Formula Weight: 380 - 420PRODUCT SPECIFICATIONSForm: Clear colorless viscous liquidDensity (d20
4): 1.12 - 1.13
Refractive Index (n20d): 1.465 - 1.469
Viscosity (20°C): 102 - 120 mpasCAS #25322-68-3
19957 500ml 1L
Polyethylene Glycol 6000, Flakes(PEG 6000)
Formula Weight: 5400 - 6600PRODUCT SPECIFICATIONSForm: White or slightly yellowish flakesSolubility (50%, Hot H
2O): Clear and colorless
Melting Point: Approx. 60°CApplications:•Differential precipitation of DNA•Enhances hybridization rate of nucleic acids•Improves efficiency of end-labeling with T4
Polynucleotide Kinase(1,2)
•Used in ligation of blunt ended DNA•Precipitates bacteriophage ± particles and
plasmid DNA•Used in preparing single-stranded M13 DNA•Promotes cell fusionPEG, with magnesium, causes DNA to undergo
a “psi” transition and it collapses into a highly condensed state(1). This results in macromolecular crowding(2) and increased efficiency in end-labeling.
References:1. Lerman, L.S. (1971) Proc Natl. Acad Sci, 68,
1886.2. Harrison, B. and Zimmerman, S. B., (1986)
Anal Biochem, 158, 307.CAS #25322-68-2
19972 500gm 1kg 5kg
Polyethylene Glycol 8000, Flakes(PEG 8000)
ULTRAPUREApprox. M.W.=8,000PRODUCT SPECIFICATIONSForm: White flakesApplications:•Differential precipitation of DNA•Enhances hybridization rate of nucleic acids•Improves efficiency of end-labeling with T4
Polynucleotide Kinase(1,2)
•Used in ligation of blunt ended DNA•Precipitates bacteriophage ± particles and
plasmid DNA•Used in preparing single-stranded M13 DNA•Promotes cell fusionPEG, with magnesium, causes DNA to undergo
a “psi” transition and it collapses into a highly condensed state(1). This results in macromolecular crowding(2) and increased efficiency in end-labeling and blunt ligation.
References:1. Lerman, L. S. (1971) Proc Natl. Acad Sci, 68,
1886.2. Harrison, B. and Zimmerman, S. B., (1986)
Anal Biochem, 158, 307-315.CAS #25322-68-3
19959 500gm 1kg 5kg
Polyethylene Glycol 8000, Powder(PEG 8000)
Molecular Weight Range: 7,000 - 9,000PRODUCT SPECIFICATIONSForm: White to off-white powderSolubility (50%, hot H
2O): Clear and colorless
Melting Point: 60 - 64°CApplications:•Differential precipitation of DNA•Enhances hybridization rate of nucleic acids•Improves efficiency of end-labeling with T4
Polynucleotide Kinase(1,2)
•Used in ligation of blunt ended DNA•Precipitates bacteriophage ± particles and
plasmid DNA•Used in preparing single-stranded M13 DNA•Promotes cell fusionPEG, with magnesium, causes DNA to undergo
a “psi” transition and it collapses into a highly condensed state(1). This results in macromolecular crowding(2) and increased efficiency in end-labeling and blunt ligation.
References:1. Lerman, L.S. (1971) Proc Natl. Acad Sci, 68,
1886.2. Harrison, B. and Zimmerman, S. B., (1986)
Anal Biochem, 158, 307.CAS #25322-68-3
19966 500gm 1kg 5kg
Biochemicals
Biochemicals
111w w w . u s b w e b . c o m
Polygalacturonic AcidCAS #25990-10-7
20595 25gm 100gm 500gm
Polymixin B SulfateUSBioAnalyzedPRODUCT SPECIFICATIONSForm: White to buff colored powderActivity: ≥ 6000 Polymixin B units/mg (dry basis) ≥ 5580 Polymixin B units/mg (as is)Identity (A, B, C): Passes testspH (5 mg/ml): 5.0 - 7.5Loss on Drying: ≤ 7.0%Content of Phenylalanine: 9%-12%CAS #1405-20-5
20551 5mu 10mu 100mu
Polyoxyethylene SorbitanSee Tween® 20, page 304See Tween 40, page 304See Tween 80, page 304
Polyvinyl Pyrrolidone (PVP)See PVP-K-15, page 279See PVP-K-30, page 279See PVP-K-90, page 280
Ponceau S, Sodium Salt
ULTRAPURE(C.I.27195)C
22H
12N
4O
13S
4Na
4
Formula Weight: 760.57Application: •As a protein stain for serum proteins separated
by agarose gel and cellulose acetate electrophoresis
CAS #6226-79-5
32819 10gm 50gm
Potassium AcetateACS Reagent GradeCH
3COOK
Formula Weight: 98.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0% (as is)pH (5%, H
2O, 25°C): 6.5 - 9.0
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.003%Phosphate (PO
4): ≤ 0.001%
Sulfate (SO4): ≤ 0.002%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmCalcium (Ca): ≤ 0.005%Magnesium (Mg): ≤ 0.002%Sodium (Na): ≤ 0.03%
20602 100gm 500gm 2.5kg
Potassium Acetate (KOAc), 3M Solution, pH 5.5
ULTRAPUREMB GradeForm: 3M solution of potassium acetate (KOAc)
in high purity dH2O, pH 5.5. Solution is 0.2 µm
filtered and dispensed into durable, square Nalgene bottles.
pH: 5.5 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75898 100ml 500ml
Potassium AspartateSee L-Aspartic Acid, Monopotassium Salt,
page 194
Potassium Chloride
ULTRAPUREACS Reagent GradeKClFormula Weight: 74.55PRODUCT SPECIFICATIONSAssay: 99.0 - 100.5%pH (5%, H
2O, 25°C): 5.4 - 8.6
Insoluble Matter: ≤ 0.005%Iodide (I): ≤ 0.002%Bromide (Br): ≤ 0.01%Chlorate & Nitrate (NO
3): ≤ 0.003%
Nitrogen Cmpds. (N): ≤ 0.001%Phosphate (PO
4): ≤ 5 ppm
Sulfate (SO4): ≤ 0.001%
Barium (Ba):Passes testHeavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 3 ppmCalcium (Ca): ≤ 0.002%Magnesium (Mg): ≤ 0.001%Sodium (Na): ≤ 0.005%CAS #7447-40-7
20598 500gm 1kg 5kg
Potassium ChlorideUSBioAnalyzedKCIFormula Weight: 74.55PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5%Identity: Passes testsAcidity or Alkalinity: Passes testLoss on Drying: ≤ 1.0%Iodate or Bromide: Passes testCalcium and Magnesium: Passes testSodium: Passes testHeavy Metals: ≤ 0.001%O.V.I.: Meets the requirementsCAS #7447-40-7
20639 500gm 2.5kg
Bioc
hem
ical
sBiochemicals
11� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Potassium Chloride (KCl), 2M Solution
ULTRAPUREMB GradeForm: 2M solution of potassium chloride (KCl) in
high purity dH2O. Solution is 0.2 µm filtered
and dispensed into durable, square Nalgene bottles.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75896 100ml
Potassium GlutamateSee L-Glutamic Acid, Potassium Salt, page 232
Potassium IodideACS Reagent GradeKIFormula Weight: 166.00PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 6.0 - 9.2
Insoluble Matter: ≤ 0.005%Loss on Drying: ≤ 0.2%Chloride and Bromide (Cl): ≤ 0.01%Iodate (IO
3): ≤ 3 pp
Nitrogen Compounds (N): ≤ 0.001%Phosphate (PO
4): ≤ 0.001%
Sulfate (SO4): ≤ 0.005%
Barium (Ba): ≤ 0.002%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 3 ppmCalcium (Ca): ≤ 0.002%Magnesium (Mg): ≤ 0.001%Sodium (Na): ≤ 0.005%
20633 100gm 500gm
Potassium IodideUSBioAnalyzedKIFormula Weight: 166.00PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.5% (dry basis)Identity: Passes testAlkalinity: Passes testLoss on Drying (105°C): ≤ 1.0%Iodate (IO
3): ≤ 4 ppm
Nitrate, Nitrite, and Ammonia: Passes testThiosulfate and Barium (Ba): Passes testHeavy Metals (Pb): ≤ 0.001%Residual Solvents: Meets the requirementsCAS #7681-11-0
20641 100gm 500gm 2.5kg
Potassium NitrateACS Reagent GradeKNO
3
Formula Weight: 101.10PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 4.5 - 8.5
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.002%Iodate (IO
3): ≤ 5 ppm
Nitrite (NO2): ≤ 0.001%
Phosphate (PO4): ≤ 5 ppm
Sulfate (SO4): ≤ 0.003%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 3 ppmCalcium (Ca): ≤ 0.005%Magnesium (Mg): ≤ 0.002%Sodium (Na): ≤ 0.005%
20271 500gm 2.5kg
Potassium Phosphate, Dibasic
ULTRAPUREACS Reagent GradeK
2HPO
4
Formula Weight: 174.18PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%pH (5%, H
2O, 25°C): 8.5 - 9.6
Insoluble Matter: ≤ 0.01%Loss on Drying: ≤ 1.0%Chloride (Cl): ≤ 0.003%Nitrogen Compds. (N): ≤ 0.001%Sulfate (SO
4): ≤ 0.005%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 0.001%Sodium (Na): ≤ 0.05%Storage: Protect from moisture (product is
deliquescent).CAS #7758-11-4
20274 100gm 500gm 1kg 2.5kg
Potassium Phosphate, Dibasic(Dipotassium Phosphate)
CP GradeK
2HPO
4
Formula Weight: 174.18PRODUCT SPECIFICATIONSForm: White deliquescent powderAssay: ≥ 98.0% K
2HPO
4 (dry basis)
Arsenic (As): ≤ 3 ppmFluoride (F): ≤ 10 ppmInsol. Substances: ≤ 0.2%Lead (Pb): ≤ 2 ppmLoss on Drying: ≤ 2.0%Note: This product is a general laboratory reagent
suitable for most laboratory animal diets where trace element deficiencies are not required. One molar stock solutions of this product may be slightly hazy. Where use of a high quality research buffer is required, USB recommends the use of our ACS Reagent Grade phosphate buffers.
Storage: Dessicate (product is deliquescent).
20276 500gm 1kg 10kg
Biochemicals
Biochemicals
113w w w . u s b w e b . c o m
Potassium Phosphate, Dibasic, Anhydrous
USBioAnalyzedK
2HPO
4
Formula Weight: 174.18PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 100.5% K
2HPO
4 (dry basis)
Identity: Passes testspH (5%, H
2O): 8.5 - 9.6
Loss on Drying: ≤ 1.0%Insoluble Substances: ≤ 0.2%Carbonate: Passes test Chloride: ≤ 0.03% Sulfate: ≤ 0.1% Arsenic: ≤ 3 ppm Iron: ≤ 0.003% Sodium: Passes test Heavy Metals: ≤ 0.001% Limit of Fluoride: ≤ 0.001% Limit of Monobasic and Tribasic Salt: Passes testStorage: Ambient, protect from moisture
32144
Potassium Phosphate, Monobasic, Anhydrous
ULTRAPUREACS Reagent GradeKH
2PO
4
Formula Weight: 136.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 4.1 - 4.5
Insoluble Matter: ≤ 0.01%Loss on Drying: ≤ 0.2%Chloride (Cl): ≤ 0.001%Nitrogen Compds. (N): ≤ 0.001%Sulfate (SO
4): ≤ 0.003%
Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.002%Sodium (Na): ≤ 0.005%CAS #7778-77-0
20227 100gm 500gm 1kg
Potassium Phosphate, MonobasicCP GradeKH
2PO
4
Formula Weight: 136.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% (dry basis)Arsenic (As): ≤ 3 ppmFluoride (F): ≤ 10 ppmInsoluble Substances: ≤ 0.2%Lead (Pb): ≤ 2 ppmLoss on Drying: ≤ 1%Note: This product is a general laboratory reagent
suitable for most laboratory animal diets where trace element deficiencies are not required. One molar stock solutions of this product may be slightly hazy. Where use of a high quality research buffer is required, USB recommends the use of our ACS Reagent Grade phosphate buffers.
20228 100gm 500gm 1kg 10kg
Potassium Phosphate Monobasic Anhydrous
USBioAnalyzedKH
2PO
4
Formula Weight: 136.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 100.5% KH
2PO
4 (dry basis)
Identity: Passes testsLoss on Drying: ≤ 1.0%Insoluble Substances: ≤ 0.2%Arsenic (As): ≤ 3 ppmLead (Pb): ≤ 0.002%Heavy Metals (as Pb): ≤ 0.002%Limit of Fluoride: ≤ 0.001%O.V.I.: Meets requirements
32147
Potassium PyruvateSee Pyruvic Acid, Potassium Salt, page 280
Potassium TelluriteK
2TeO
3
Formula Weight: 253.79PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 96.0%Identity: By IRSolubility (1%, H
2O): Completely soluble
CAS #7790-58-1
20635 10gm 25gm 100gm
Potassium ThiocyanateACS Reagent GradeKSCNFormula Weight: 97.18PRODUCT SPECIFICATIONSForm: Colorless or white crystalsAssay: ≥ 98.5%pH (5%, H
2O, 25°C): 5.3 - 8.7
Insoluble in Water: ≤ 0.005%Chloride (Cl): ≤ 0.005%Sulfate (SO
4): ≤ 0.005%
Ammonium (NH4): ≤ 0.003%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 2 ppmSodium (Na): ≤ 0.005%Iodine-Consuming Substances: Passes test
20273 500gm 2.5kg
PPO(2,5-Diphenyloxazole)
C15
H11
NOFormula Weight: 221.25PRODUCT SPECIFICATIONSForm: Fine, white powderAssay: ≥ 99% Identity: By IRMelting Point: 70 - 72°CLoss On Drying: ≤ 0.3% Residue On Ignition: ≤ 0.01%CAS #92-71-7
20642 25gm 50gm 100gm 500gm 1kg
Bioc
hem
ical
sBiochemicals
11� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
PrednisoloneUSBioAnalyzedC
21H
28O
5
Formula Weight: 360.47PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderIdentity: (A, B): Passes testsAssay: 97.0 - 102.0% (dry basis)[α]25
D : + 97° to +103° (c=1 in dioxane)
Loss on Drying: ≤ 1.0%Residue on Ignition: NegligibleSelenium: ≤ 0.003%Ordinary Impurities: Passes test
20650 1gm 10gm 25gm
ProgesteroneUSBioAnalyzedC
21H
30O
2
Formula Weight: 314.46PRODUCT SPECIFICATIONSForm: White to cream colored powderAssay: 97.0 - 103.0% (dry basis)Identity: By IR[α]25
D : +175° to +183° (c=2 in dioxane,
dry basis)Melting Point: 126 - 131°CLoss on Drying: ≤ 0.5%CAS #57-83-0
20665 5gm 25gm 100gm
D-ProlineC
5H
9NO
2
Formula Weight: 115.13CAS #344-25-2
20723 1gm 5gm 10gm
DL-ProlineC
5H
9NO
2
Formula Weight: 115.13CAS #609-36-9
20725 5gm 25gm 100gm
L-ProlineUSBioAnalyzed(C
4H
8N)COOH
Formula Weight: 115.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IRChromotographic Purity: Passes test[α]20
D : -84.3° to -86.3° (c=4 in H
2O)
Loss on Drying: ≤ 0.4%Residue on Ignition: ≤ 0.4%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 0.0015%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Chromatographic Purity: Meets requirementsO.V.I.: Meets requirementsCAS #147-85-3
20730 10gm 25gm 100gm 1kg
L-Proline-p-NitroanilideC
11H
12N
3O
3
Formula Weight: 234.23Storage: -20°CCAS #7369-91-7
20740 500mg 1gm 5gm
r-hProMMP-7(Promatrilysin)
See Human Promatrilysin, Recombinant, page 241
Propionic Acid, Calcium Salt(CH
3CH
2COO)
2Ca
Formula Weight: 186.22CAS #4075-81-4
20765 500gm 1kg
Propionic Acid, Sodium Salt(Sodium Propionate)
CH3CH
2COONa
Formula Weight: 96.06PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5%Identity: By IR Moisture (KF): ≤ 1%Arsenic (As): ≤ 2 ppmIron (Fe) ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #137-40-6
20766 1kg
n-Propyl Gallate(HO)
3C
6H
2COOCH
2CH
2CH
3
Formula Weight: 212.20PRODUCT SPECIFICATIONSForm: Beige powderAssay (UV): 98 - 102% (as is)Identity: By IR Melting Point: 146° - 150°CCAS #121-79-9
20770 100gm 500gm
Protamine SulfateSource: SalmonPRODUCT SPECIFICATIONSForm: White to off-white powderAssay: Each mg neutralizes not less than 100
USP Heparin Units (dry basis)Loss on Drying: ≤ 5.0%Nitrogen (N): 22.5 - 25.5% (dry basis)Sulfate (SO
4): 16.0 - 22.0% (dry basis)
CAS #9009-65-8
20795 5gm 25gm 100gm
Biochemicals
Biochemicals
115w w w . u s b w e b . c o m
Protein Determination Reagent
ULTRAPUREForm: One bottle of Protein Determination
Reagent is sufficient for 500 standard assays or 2500 microassays
Application: •This protein dye reagent allows rapid and
simple determination of the protein content of various solutions. This reagent is based on the Bradford(1,2) protein assay method and is supplied as a five-fold concentrate. It can be used either in a standard assay procedure for protein concentrations between 100 µg/ml and 1 mg/ml or in a microassay procedure for concentrations between 1 µg/ml and 10 µg/ml. The assay procedure consists of mixing the reagent with sample and blank, incubating (usually 5 - 20 min), and measuring the absorbance of the sample and blank at 595 nm. The assay procedure is supplied with every bottle.
References:1. Bradford, M. M. (1976) Anal. Biochem. 72,
248-254.2. Bradford, M. M. and Williams, W. L. (1977) U.S.
Patent No. 4,023,933.
30098 500ml
ProteinaseSource: Bacillus subtilisPRODUCT SPECIFICATIONSForm: White crystalline powderUnit Definition: One unit is that amount of enzyme
which produces in one minute, under the conditions of the test, a hydrolysate whose absorbance at 275 nm is the same as that of a tyrosine solution containing 1.50 µg tyrosine per ml.
20815 50gm 100gm
Proteinase KSource: Tritirachium albumPRODUCT SPECIFICATIONSForm: Lyophilized powderConcentration: 20 - 50 units/mgMolecular Weight: 28.9 kDaUnit Definition: One unit is the amount of enzyme
that liberates folin positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 min at 37°C using hemoglobin as substrate.
Application: •Proteinase K inactivates DNase and RNase from
most mammalian cells and microorganisms in the presence of 0.5 - 1% SDS. It can also be used for research involving protein structure because of its cleavage specificity.
Storage: 4°C
76230Y 100mg76230Z 500mg76230 1gm(2x500mg)
pUC 18 DNASee Molecular Biology Products & Kits Section,
page 92
pUC 19 DNASee Molecular Biology Products & Kits Section,
page 92
Purification SolutionsPhenol, pH 4.5, Equilibrated, page 271Phenol, pH 8.0, Equilibrated, page 271Phenol:Chloroform:Isoamyl Alcohol (25:24:1),
page 271
Purine, Free BaseC
5H
4N
4
Formula Weight: 120.11CAS #120-73-0
20835 250mg 1gm
Putrescine, Dihydrochloride(1,4-Diamino-Butane Dihydrochloride)
NH2(CH
2)4NH
2•2HCl
Formula Weight: 161.07PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 99.0%Identity: By IRChromatography (TLC): HomogeneousStorage: -20°C, protect from moisture.CAS #333-93-7
20850 5gm 25gm
PVP-K-15(Polyvinylpyrrolidone)
Formula Weight: 10,000 (average)PRODUCT SPECIFICATIONSForm: Off-white powderActive content: ≥ 95%K-value (viscosity of 1% solids w/v solution):
13 - 19Moisture: ≤ 5.0%% Unsaturation (calculated as % vinyl
pyrrolidone): ≤ 0.5%
20610 100gm 500gm 1kg
PVP-K-30(Polyvinylpyrrolidone)
Formula Weight: 40,000 (average)PRODUCT SPECIFICATIONSForm: Off-white powderActive content: ≥ 95%K-value (viscosity of 1% solids w/v aqueous
solution): 26 - 34Moisture: ≤ 5.0%% Unsaturation (calculated as %
vinylpyrrolidone): ≤ 0.5%CAS #9003-39-8
20611 1kg
Bioc
hem
ical
sBiochemicals
116 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
PVP-K-90(Polyvinylpyrrolidone)
ULTRAPUREFormula Weight: 360,000 (average)PRODUCT SPECIFICATIONSForm: Off-white powderActive content: ≥ 95%K-value (viscosity of 1% solids w/v aqueous
solution): 80 - 100Moisture (KF): ≤ 5.0%% Unsaturation (calculated as %
vinylpyrrolidone): ≤ 0.5%Application:•Used in nucleic acid hybridization buffer
20612 100gm 1kg
Pyridoxal-5'-Phosphate, Monohydrate(Pyridoxyl-5'-Phosphoric Acid; Codecarboxylase)
C8H
10NO
6P•H
2O
Formula Weight: 265.16PRODUCT SPECIFICATIONSForm: White to pale yellow powderAssay: ≥ 98.0%Identity: By IRMoisture: 6 - 9%Free Phosphate (PO
4): ≤ 1.0%
Pyridoxal: ≤ 0.5%CAS #41468-25-1
20895 1gm 5gm 25gm
Pyridoxamine, Dihydrochloride, Monohydrate
C8H
12N
2O
2•2HCl•H
2O
Formula Weight: 259.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97%Storage: -20°C, protect from moisture.CAS #524-36-7
20925 1gm
Pyridoxine, Hydrochloride(Vitamin B
6 ; Pyridoxyl HCl)
USBioAnalyzedC
8H
11NO
3•HCl
Formula Weight: 205.64PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 102.0% (dry basis)Identity (A, B): Passes testsLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.003%Chloride (Cl): 16.9 - 17.6% (dry basis)O.V.I.: Meets requirementsCAS #58-56-0
20900 10gm 25gm 50gm 100gm 500gm
Pyrimidine, Free BaseC
4H
4N
2
Formula Weight: 80.09Liquid-AmpulesCAS #289-95-2
20940 500mg
Pyruvate Kinase(E.C.2.7.1.40)Source: Rabbit MusclePRODUCT SPECIFICATIONSForm: Lyophilized, salt freeActivity: ≥ 250 units/mgUnit Definition: One unit oxidizes 1 µmol NADH/min at 25°C, pH 7.4.Contaminating Activities: CK: < 0.005% LDH: < 0.005% GK: < 0.005%Storage: -20°CCAS #9001-59-6
20975 300units 1,500units 15,000units
Pyruvate Kinase(E.C.2.7.1.40)Source: Rabbit MusclePRODUCT SPECIFICATIONSForm: Ammonium sulfate suspensionActivity: ≥ 200 units/mg proteinContaminating Activities: MK: ≤ 0.01% α-GDH: ≤ 0.01% Enolase: ≤ 0.01% GAPDH: ≤ 0.01% PGM: ≤ 0.01% LDH: ≤ 0.02%Unit Definition: One unit reduces 1 µmol of
phosphoenolpyruvate/min at 25°C, pH 7.5.Note: The above product is available in
manufacturing quantities. Please inquire for bulk pricing.
Storage: 4°C
20977 1,000units 5,000units 10,000units
Pyruvic Acid, Free AcidCH
3COCOOH
Formula Weight: 88.06Storage: 4°CCAS #127-17-3
20985 10gm 25gm 100gm
Pyruvic Acid, Potassium Salt(Potassium Pyruvate)
Reagent GradeC
3H
3O
3K
Formula Weight: 126.15PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IRAssay: ≥ 98%Solubility (10%, H
2O): Passes test
20990 10gm 25gm 100gm
Biochemicals
Biochemicals
117w w w . u s b w e b . c o m
Pyruvic Acid, Sodium Salt(Sodium Pyruvate)
CH3COCOONa
Formula Weight: 110.04PRODUCT SPECIFICATIONSForm: White to slightly yellow crystalline powderAssay: ≥ 98.5%Identity: By IRMoisture (KF): ≤ 1.0%Chloride (Cl): ≤ 30 ppmSulfate (SO
4): ≤ 0.01%
Heavy Metals (Pb): ≤ 10 ppmCAS #113-24-6
20995 5gm 10gm 25gm 100gm 500gm
Quercetin, DihydrateC
15H
10O
7•2H
2O
Formula Weight: 338.27PRODUCT SPECIFICATIONSForm: Bright yellow powderIdentity: By IRMoisture (KF): ≤ 14.0%CAS #6151-25-3
21000 25gm 100gm
D-(+)-Raffinose, PentahydrateC
18H
32O
16•5H
2O
Formula Weight: 594.51PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : +104.9° ± 1.3° (c=4 in H
2O & NH
3)
(corrected to a theoretical pentahydrate)Loss on Drying: ≤ 15.3%Residue on Ignition: ≤ 0.05%Iron (Fe): ≤ 5 ppmHeavy Metals (Cu): ≤ 10 ppmArsenic (As): ≤ 0.5 ppmCAS #17629-30-0
21060 25gm 100gm 500gm 1kg
RapidGelTM DNA Sequencing Gel Solutions
ULTRAPUREMB GradeForm: Pre-mixed, liquid acrylamide in ready-to-
use form for Ultrapure DNA sequencing gels. Each formulation contains 7M urea and either TBE buffer.
Testing: Functionally tested in sequencing gel electrophoresis.
To use: De-gas solution by placing under gentle vacuum for 10 min at room temperature. Add 600 µl 10% ammonium persulfate and 60 µl TEMED per 100 ml gel solution to polymerize. Allow to polymerize at room temperature from one hour to overnight. For polymerization within 30-45 min, add 1 ml 10% ammonium persulfate and 100 µl TEMED per 100 ml gel solution. Pour gel immediately. Be sure to match gel with TBE running buffer, PN 75891
Note: Urea may crystallize out of solution when the gel mix is stored as recommended at 4°C. This is normal and will not affect performance. If this occurs, warm the gel mix at 37°C for 10 min or until the urea redissolves. Mix gently. Cool to room temperature before adding the ammonium persulfate and TEMED.
Storage: 4°CRapidGel Formulations:
RapidGel4%LiquidAcrylamide,TBE75841 500mlRapidGel6%LiquidAcrylamide,TBE75843 500mlRapidGel8%LiquidAcrylamide,TBE75844 500ml
RapidGelTM 40% Liquid Acrylamide Stock Solution
ULTRAPUREMB GradeForm: Pre-mixed, 40% liquid acrylamide
(19:1 acrylamide:bis-acrylamide ratio) in ready-to-use form for Polyacrylamide Gel Electrophoresis (PAGE). Stock solution provides flexibility for different acrylamide percentages in a wide variety of gel applications. This formulation does not contain urea.
Testing: Functionally tested in gel electrophoresis.
To use: Dilute 40% stock solution to desired concentration with appropriate running buffer for PAGE. To make 100 ml of a sequencing gel solution containing 7-8.3M urea, add an appropriate volume of 40% RapidGel solution, 10 ml of 10X (or 5 ml of 20X) running buffer, 42-50 gm of urea and high purity dH
2O to
100 ml in a clean, graduated cylinder. Stir and warm solution at 35-45°C to dissolve urea. (Do not microwave.) Cool to room temperature. Filter through a 0.2 µm filter and leave under gentle vacuum for 10 min to de-gas. Add 600 µl 10% ammonium persulfate and 60 µl TEMED per 100 ml gel solution to polymerize. Allow to polymerize at room temperature from one hour to overnight. For polymerization within 30-45 min, add 1 ml 10% ammonium persulfate and 100 µl TEMED per 100 ml gel solution. Pour gel immediately.
Storage: 4°C
75848 500ml
Bioc
hem
ical
sBiochemicals
118 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
RapidGelTM-XL 6% Liquid Acrylamide DNA Sequencing Gel, TBE Formulation
ULTRAPUREMB GradeForm: Pre-mixed, liquid acrylamide in ready-
to-use form for Ultrapure DNA sequencing gels. Contains TBE Buffer and 7M urea. The most commonly requested gel formulation, RapidGel-XL, 6%, is a high resolution matrix for DNA sequencing, yielding up to 40% more readable sequence per gel when using either radioactive or fluorescent labeling methods. No gel fixing is necessary.
Testing: Functionally tested in sequencing gel electrophoresis.
To use: Intended for use with 1X TBE Buffer. De-gas solution by placing under gentle vacuum for 10 min at room temperature. Add 600 µl 10% ammonium persulfate and 60 µl TEMED per 100 ml gel solution to polymerize. Allow to polymerize at room temperature from one hour to overnight. For polymerization within 30-45 min, add 1 ml 10% ammonium persulfate and 100 µl TEMED per 100 ml gel solution. Pour gel immediately. Do not fix gel.
Note: Urea may crystallize out of solution when the gel mix is stored as recommended at 4°C. This is normal and will not affect performance. If this occurs, warm the gel mix at 37°C for 10 min or until the urea redissolves. Mix gently. Cool to room temperature before adding the ammonium persulfate and TEMED.
Use with corresponding TBE Running Buffer: 10X Ready Mixed Powder, PN 70454, packaged in 6 x 200 ml bottles or TBE Buffer, 5X Solution, PN 75891, packaged in 1 and 5 liter sizes.
Storage: 4°C
75861 500ml
RapidGelTM-XL 40% Liquid Acrylamide Stock Solution
ULTRAPUREMB GradeForm: Pre-mixed, 40% liquid acrylamide in
ready-to-use form for Polyacrylamide Gel Electrophoresis (PAGE). Stock solution provides flexibility for different acrylamide percentages in a wide variety of gel applications. RapidGel-XL is a high resolution matrix for gel electrophoresis. When used for sequencing gels, up to 40% more readable sequence per gel may be achieved. No gel fixing is necessary. This formulation does not contain urea.
Testing: Functionally tested in sequencing gel electrophoresis.
To use: RapidGel-XL is used in the same way as regular RapidGel 40% Liquid Acrylamide Stock Solution except there is no need to fix the gel.
Storage: 4°C
75863 100ml 500ml
RapidRunTM Agarose Buffer, 20X Solution
ULTRAPUREMB GradeForm: RapidRun Agarose Buffer allows for gels
to be run in one-fourth the time required for standard buffers, such as TAE and TBE. The buffer is a non-borate weak acid that enables high voltage and more rapid electrophoretic runs. Temperature seldom exceeds 38°C during high voltage runs, minimizing the band diffusion and gel degradation commonly associated with TAE and TBE under the same high voltage conditions.
20X RapidRun Agarose Buffer consists of 1.78M Tris, 0.57M Taurine, and 0.01M EDTA. The solution is 0.2 µm filtered.
pH (1:20, 25°C): 8.9 + 0.1Conductivity (1X): 830 + 100 µmhos/cmTesting: Meets or exceeds functional test criteria
for high voltage (300v, 15 min) mini-agarose gel electrophoresis.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testApplications:1. High voltage, fast agarose gel electrophoresis
(20-30 volts/cm).2. This buffer is compatible with commercial
gel extraction kits. However, the pH should be adjusted with sodium acetate, pH 5.0, as recommended by the manufacturer.
Note: 1 ml of RapidRun Loading Dye (PN 77524) is included with each pack size. The dye front migrates lower than 50 bases.
Storage: Ambient. Crystals which form at low temperatures can be re-dissolved by warming briefly.
77523 1L 5L
RapidRunTM Loading Dye
ULTRAPUREMB GradeForm: 5X loading dye (0.05% dye in 50%
glycerol).Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testApplication: •Used as a loading dye designed for high voltage
agarose gel electrophoresis with buffers such as RapidRun Agarose Buffer (PN 77523). It may also be used for standard voltage gel electrophoresis. RapidRun Loading Dye develops an intense orange color in the presence of RapidRun Agarose Buffer. The dye front migrates lower than 50 bases.
77524 1ml 5ml
ReserpineC
33H
40N
2O
9
Formula Weight: 608.68CAS #50-55-5
21110 1gm
ResorcinolC
6H
4(OH)
2
Formula Weight: 110.11CAS #108-46-3
21115 500gm
L-Rhamnose Monohydrate(6-Deoxy-L-Mannose)
C6H
12O
5•H
2O
Formula Weight: 182.17PRODUCT SPECIFICATIONSForm: White powderIdentity: By IR[α]20
D : +8.5° - +9.5°
Moisture (KF): ≤ 11.5%Residue on Ignition: ≤ 0.1%Solubility: Passes testIron (Fe): ≤ 5 ppmArsenic (As): ≤ 0.5 ppmHeavy Metals (Cu): ≤ 10 ppmCAS #10030-85-0
21121 5gm 10gm 25gm 500gm
Biochemicals
Biochemicals
119w w w . u s b w e b . c o m
Rhodamine-B-IsothiocyanateC
29H
30N
3O
3SCl
Formula Weight: 536.08Storage: -20°CCAS #36877-69-7
21125 100mg 1gm 5gm
Riboflavin(Vitamin B
2 ; Vitamin G)
USBioAnalyzedC
17H
20N
4O
6
Formula Weight: 376.36PRODUCT SPECIFICATIONSForm: Yellow to orange/yellow crystalline powderAssay: 98.0 - 102.0% (dry basis)Identity: By IR[α]25
D : -115° to -135° (c=0.5 in 0.05M NaOH)
Loss on Drying: ≤ 1.5%Residue on Ignition: ≤ 0.3%Limit of Lumiflavin: Passes testO.V.I.: Meets requirementsCAS #83-88-5
21145 25gm 100gm 500gm 1kg
Riboflavin-5'-Phosphate, Sodium Salt(Flavin Mononucleotide; FMN)
USBioAnalyzedC
17H
20N
4O
9PNa•2H
2O
Formula Weight: 514.36 (dihydrate)PRODUCT SPECIFICATIONSForm: Yellow crystalline powderAssay (fluorometric): 73.0 - 79.0% C
17H
20N
4O
6
(dry basis)Identity (A, B): Passes testspH (1%, H
2O): 5.0 - 6.5
[α]25D : +37.0° to +42.0° (c=1.5 in 5N HCl)
Loss on Drying: ≤ 7.5%Residue on Ignition: ≤ 25.0%Lumiflavin: Passes testFree Phosphate: Passes test (≤ 1.0%)Free Riboflavin and Riboflavin Diphosphates:
≤ 6.0% eachO.V.I.: Meets requirementsCAS #130-40-5
21150 10gm 25gm 100gm 500gm
D-(–)-RiboseC
5H
10O
5
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 98.0% (dry basis)[α]20
D : -20.4° ± 0.4° (c=2 in H
2O, NH
3)
Loss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Arsenic (As): ≤ 0.5 ppmIron (Fe): ≤ 5 ppmHeavy Metals (Cu): ≤ 10 ppmCAS #50-69-1
21220 5gm 10gm 25gm 100gm
D-(–)-Ribose-5-Phosphate, Disodium Salt
C5H
9O
8PNa
2
Formula Weight: 274.07Storage: -20°CCAS #18265-46-8
21235 250mg 500mg 1gm 5gm
RifampinC
43H
58N
4O
12
Formula Weight: 822.94PRODUCT SPECIFICATIONSForm: Reddish brown crystalline powderAssay: 95.0 - 103.0% of C
43H
58N
4O
12 (dry basis)
93.0 - 103.0% of C43
H58
N4O
12 (as is)
Identity: By IRpH (1%, H
2O): 4.5 - 6.5
Loss on Drying: ≤ 2.0%Related Substances: Passes testMode of Action: Inhibits RNA synthesis by
binding to and inhibiting the β subunit of RNA polymerase.
CAS #13292-46-1
21246 1gm 5gm
RNA, Free AcidSource: Torula YeastPRODUCT SPECIFICATIONSForm: White powderMoisture: ≤ 8.0%Nitrogen: ≥ 15.3% (dry basis)Phosphorous: ≥ 9.0% (dry basis)
21185 25gm 100gm 200gm 1kg
RNA, Sodium SaltSource: YeastPRODUCT SPECIFICATIONSForm: White powderMoisture: 4.0 - 8.0%Phosphorous: ≥ 8.0%Nitrogen: ≥ 14.5%pH (1% H
2O): 6.5 ± 0.5
Biuret test: NegativeCAS #73049-77-1
21190 25gm 100gm 1kg
RNA Solutions Kit
ULTRAPUREMB GradeForm: A selection of 8 Convenience Reagent
Solutions, 100 ml each. 0.5M EDTA, PN 15694 2M KCl, PN 75896 1M MgCl
2, PN 78641
5M NaCl, PN 75888 5M NH
4OAc, PN 75901
1M Tris, pH 7.0, PN 22637 1M Tris, pH 8.0, PN 22638 Water, RNase-Free, DEPC Treated, PN 70783
75903 1Kit
RNase A, Lyophilized(E.C. 3.1.4.22)Source: Bovine pancreasForm: Lyophilized powderUnit Definition:One unit is that amount of enzyme
causing the hydrolysis of RNA at a rate such that K equals unity at 25°C and pH 5.0.
Storage: -20°C, desiccated
21195 100mg 1gm 5gm
Bioc
hem
ical
sBiochemicals
1�0 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Safflower Oil
21310 500ml
SalicinC
13H
18O
7
Formula Weight: 286.28PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IR[α]20
D : -61° ± 2° (c=5 in H
2O)
Melting Point: 195 - 205°CLoss on Drying: ≤ 0.3%CAS #138-52-3
21315 5gm 10gm 25gm 100gm 250gm
SAMe-PTSSee S-Adenosyl-L-Methionine Sulfate
p-Toluenesulfonate, page 175
SaponinPRODUCT SPECIFICATIONSForm: Tan powderIdentity: By IRSolubility (20% in H
2O): Clear to hazy solution
pH (20% in H2O): 4.50 ± 0.3
Residue on Ignition: ≤ 10%
21435 100gm
SDSSee Sodium Dodecyl Sulfate, page 286
Sequencing GelsSee DNA Sequencing Gel Mix, page 222See RapidGel Solutions, page 281
D-SerineHOCH
2CH(NH
2)COOH
Formula Weight: 105.09CAS #312-84-5
21490 5gm 25gm
DL-SerineCH
2OHCHNH
2COOH
Formula Weight: 105.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 10 ppmChloride (Cl): ≤ 0.021%Sulfate (SO
4): ≤ 0.05%
Lead (Pb): ≤ 5 ppmCAS #302-84-1
21485 50gm 100gm 1kg 5kg
DL-Serine (Non-Animal)CH
2OHCHNH
2COOH
Formula Weight: 105.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRLoss On Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.021%Sulfate (SO
4): ≤ 0.05%
Heavy Metals (Pb): ≤ 20 ppm
21486 50gm 100gm 1kg
L-SerineUSBioAnalyzedHOCH
2CH(NH
2)COOH
Formula Weight: 105.09PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IR[α]25
D : +14.0° to +15.6° (c=10 in 2N HCl)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 0.0015%Chromographic Purity: Passes testO.V.I.: Meets the requirementCAS #56-45-1
21505 25gm 100gm 1kg
-SitosterolC
29H
50O
Formula Weight: 414.71PRODUCT SPECIFICATIONSForm: White to pale yellow fine powderIdentity: By IR[α]25
D : -24° to -38° (c=2 in CHCl
3)
Loss on Drying: ≤ 2%Melting Point: 135 - 142°Cβ-Sitosterol-1 Content: ≥ 50%CAS #83-46-5
21600 25gm 100gm 500gm
Sodium Acetate, Anhydrous
ULTRAPUREACS Reagent GradeCH
3COONa
Formula Weight: 82.03PRODUCT SPECIFICATIONSForm: White powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 7.0 - 9.2
Insoluble Matter: ≤ 0.01%Loss on Drying: ≤ 1.0%Chloride (Cl): ≤ 0.002%Phosphate (PO
4): ≤ 0.001%
Sulfate (SO4): ≤ 0.003%
Calcium (Ca): ≤ 0.005%Magnesium (Mg): ≤ 0.002%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%Application: •Used in the precipitation of DNACAS #127-09-3
21608 500gm 1kg
Sodium Acetate (NaOAc), 3M Solution, pH 5.5
ULTRAPUREMB GradeForm: 3M solution of sodium acetate (NaOAc) in
high purity dH2O, pH 5.5. Solution is 0.2 µm
filtered and dispensed into durable, square Nalgene bottles.
pH: 5.5 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75897 100ml 500ml
Sodium AspartateSee Aspartic Acid, Sodium Salt, page 195
Biochemicals
Biochemicals
1�1w w w . u s b w e b . c o m
Sodium Azide
ULTRAPURENaN
3
Formula Weight: 65.01PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Loss on Drying: ≤ 0.5%CAS #26628-22-8
21610 100gm 500gm
Sodium BenzoateSee Benzoic Acid, Sodium Salt, page 196
Sodium BicarbonateACS Reagent GradeNaHCO
3
Formula Weight: 84.01PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.7 - 100.3%Insoluble Matter: ≤ 0.015%Chloride (Cl): ≤ 0.003%Phosphate (PO
4): ≤ 0.001%
Sulfur Compounds (SO4): ≤ 0.003%
Ammonium (NH4): ≤ 5 ppm
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 0.001%Calcium (Ca): ≤ 0.02%Magnesium (Mg): ≤ 0.005%Potassium (K): ≤ 0.005%
21602 500gm 2.5kg
Sodium Bisulfite(Mixture of Sodium Bisulfite and Sodium Metabisulfite)
ACS Reagent GradeNaHSO
3 and Na
2S
2O
5
PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 58.5% (SO
2)
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.02%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.002%
21603 500gm 2.5kg
Sodium BorateSee Boric Acid, Sodium Salt, page 198
Sodium BorohydrideNaBH
4
Formula Weight: 37.83PRODUCT SPECIFICATIONSPurity: ≥ 98%Reducing agent for cytochrome and many other
biochemicals.Contains 0.5% magnesium carbonate to prevent
cakingCAS #16940-66-2
21615 25gm 100gm
Sodium CacodylateSee Cacodylic Acid, Sodium Salt, page 201
Sodium Carbonate, AnhydrousACS Reagent GradeNa
2CO
3
Formula Weight: 105.99PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.5%Insoluble Matter: ≤ 0.01%Loss on Drying (285°C): ≤ 1.0%Chloride (Cl): ≤ 0.001%Nitrogen Compounds (N): ≤ 0.001%Phosphate (PO
4): ≤ 0.001%
Silica (SiO4): ≤ 0.005%
Sulfur Compounds (SO4): ≤ 0.003%
Ammonium Hydroxide Precipitate: ≤ 0.01%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmCalcium (Ca): ≤ 0.03%Magnesium (Mg): ≤ 0.005%Potassium (K): ≤ 0.005%
21616 500gm 2.5kg
Sodium CaseinateSee Casein, Sodium, page 203
Sodium Chloride (NaCl), 5M Solution
ULTRAPUREMB GradeForm: 5M solution of sodium chloride (NaCl) in
high purity dH2O. Solution is 0.2 µm filtered
and dispensed into durable, square Nalgene bottles.
Contaminant Testing: Heavy Metals: ≤ 5 ppm DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
75888 100ml 500ml 1L
Sodium Chloride
ULTRAPUREACS Reagent GradeNaClFormula Weight: 58.44PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 5.0 - 9.0
Insoluble Matter: ≤ 0.005%Iodide (I): ≤ 0.002%Bromide (Br): ≤ 0.01%Chlorate & Nitrate (NO
3): ≤ 0.003%
Nitrogen Cmpds. (N): ≤ 0.001%Phosphate (PO
4): ≤ 5 ppm
Sulfate (SO4): ≤ 0.004%
Barium (Ba): Passes testHeavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 2 ppmCalcium (Ca): ≤ 0.002%Magnesium (Mg): ≤ 0.001%Potassium (K): ≤ 0.005%Application: •Used for the precipitation of DNACAS #7647-14-5
21618 500gm 1kg 5kg 10kg
Bioc
hem
ical
sBiochemicals
1�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Sodium ChlorideUSBioAnalyzedNaClFormula Weight: 58.44PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: 99.0 - 100.5% (dry basis)Appearance of Solution: Clear and colorlessIdentity: Passes testAcidity or Alkalinity: Passes testLoss on Drying: ≤ 0.5%Iodides: Passes testMagnesium and Alkaline-Earth Metals: ≤ 0.01%Arsenic (As): ≤ 1 ppmBarium (Ba): Passes testFerrocyanides: Passes testSulfate (SO
4): ≤ 0.020%
Iron (Fe): ≤ 2 ppmNitrites: Passes testHeavy Metals (Pb): ≤ 5 ppmLimit of Bromides: ≤ 0.010%Limit of Phosphates: ≤ 0.0025%
21629 500gm 2.5kg 10kg
Sodium CholateSee Cholic Acid, Sodium Salt, page 206
Sodium CitrateSee Citric Acid, Trisodium Salt, page 207
Sodium DeoxycholateSee Deoxycholic Acid, Sodium Salt, page 214
Sodium DiatrizoateC
11H
8I3N
2NaO
4
Formula Weight: 635.88For density gradient studies.CAS #737-31-5
21645 50gm 100gm 1kg
Sodium Dodecyl Sulfate (SDS)(Sodium Lauryl Sulfate)
ULTRAPURECH
3(CH
2)11
OSO3Na
Formula Weight: 288.38PRODUCT SPECIFICATIONSForm: White crystalline flakesAssay: ≥ 99% (acidity)Assay: ≥ 98% (GC)A (280 nm, 3%, 1 cm): ≤ 0.1A (230 nm, 3%, 1 cm): ≤ 0.2Loss on Drying: ≤ 1%Insoluble Matter: ≤ 0.003%Chloride (Cl): ≤ 0.01%Phosphate (PO
4): ≤ 1 ppm
Copper (Cu): ≤ 5 ppmIron (Fe): ≤ 1 ppmLead (Pb): ≤ 5 ppmProtocol for 10% SDS Solution: SDS: 100 gm H
2O: 900 ml
Heat to 68° to dissolve. Adjust to pH 7.2 and bring to 1 liter. There is no need to sterilize 10% SDS.
CAS #151-21-3
75819 100gm 1kg
Sodium Dodecyl Sulfate (SDS)(Sodium Lauryl Sulfate)
CH3(CH
2)11
OSO3Na
Formula Weight: 288.38PRODUCT SPECIFICATIONSForm: White crystalline powderIdentity: By IRAssay: ≥ 95% (dry basis)Solubility (20%, H
2O): Clear, faintly yellow solution
CAS #151-21-3
18220 100gm 500gm 1kg 5kg
Sodium Dodecyl Sulfate (SDS), 10% Solution
ULTRAPUREForm: 10% (w/v) solution of sodium dodecyl
sulfate (SDS) in high purity dH2O. Solution is
0.2 µm filtered and dispensed into durable, square Nalgene bottles.
A (280 nm, 3%, 1 cm): ≤ 0.1A (230 nm, 3%, 1 cm): ≤ 0.2Note: Precipitation of SDS is not unusual. Warm
gently to redissolve.
77504 100ml 500ml 1L
Sodium Dodecyl Sulfate (SDS), 20% Solution
ULTRAPUREForm: 20% (w/v) solution of sodium dodecyl
sulfate (SDS) in high purity dH2O. Solution is
0.2 µm filtered and dispensed into durable, square Nalgene bottles.
Note: Precipitation of SDS is not unusual. Warm gently to redissolve.
75832 100ml 500ml
Sodium GlutamateSee Glutamic Acid, Monosodium Salt, page 232
Sodium LactateSee DL-Lactic Acid, Sodium Salt, page 246
Sodium-N-Lauryl SarcosineSee N-Lauryl Sarcosine, Sodium Salt, page 246
Sodium Lauryl SulfateSee Sodium Dodecyl Sulfate, page 286
Sodium NitrateACS Reagent GradeNa
2NO
3
Formula Weight: 84.99PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 5.5 - 8.3
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.001%Iodate (IO
3): ≤ 5 ppm
Nitrate (NO2): ≤ 0.001%
Phosphate (PO4): ≤ 5 ppm
Sulfate (SO4): ≤ 0.003%
Calcium (Ca): ≤ 0.005%Magnesium (Mg): ≤ 0.002%Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 3 ppm
21606 500gm 2.5kg
Biochemicals
Biochemicals
1�3w w w . u s b w e b . c o m
Sodium OxamateSee Oxamic Acid, Sodium Salt, page 267
Sodium Phosphate, Dibasic AnhydrousACS Reagent GradeHygroscopicNa
2HPO
4
Formula Weight: 141.96PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 8.7 - 9.3
Insoluble Matter: ≤ 0.01%Loss on Drying : ≤ 0.2%Chloride (Cl): ≤ 0.002%Sulfate (SO
4): ≤ 0.005%
Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.002%Storage: Store ambient, protect from moisture
(product is hygroscopic).
21660 500gm 2.5kg
Sodium Phosphate, DibasicUSBioAnalyzedNa
2HPO
4
Formula Weight: 141.96PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 100.5% (dry basis)Identity: Passes testsLoss on Drying (130°C): ≤ 5.0%Insoluble Substances: ≤ 0.4%Chloride: ≤ 0.06%Sulfate: ≤ 0.2%Arsenic: ≤ 16 ppmHeavy Metals (Pb): ≤ 0.002%Solubility (1%): Completely soluble in 15 minutes;
clear and colorless solutionpH (1.0%, 25°C): 8.4 - 9.2Identity: By IRLoss on Drying (105°C): ≤ 5.0%Storage: Store ambient, protect from moisture
(product is hygroscopic).
32125 100gm 500gm 2.5kg
Sodium Phosphate, Dibasic(Disodium Phosphate)
CP GradeNa
2HPO
4
Formula Weight: 141.96PRODUCT SPECIFICATIONSForm: White, hygroscopic, crystalline powderAssay: ≥ 98.0% (dry basis)Arsenic (As): ≤ 3 ppmFluoride (F): ≤ 0.005%Heavy Metals (Pb): ≤ 10 ppmInsoluble Substances: ≤ 0.2%Loss on Drying: ≤ 5%Note: This product is a general laboratory reagent
suitable for most laboratory animal diets where trace element deficiencies are not required. One molar stock solutions of this product may be slightly hazy. Where use of a high quality research buffer is required,USB recommends the use of our ACS reagent grade phosphate buffers.
Storage: Store ambient, protect from moisture (product is hygroscopic).
20226 500gm 1kg 5kg
Sodium Phosphate, Dibasic, Heptahydrate
ULTRAPUREACS Reagent GradeEffloresces in warm dry airNa
2HPO
4•7H
2O
Formula Weight: 268.07PRODUCT SPECIFICATIONSForm: White powderAssay: 98.0 - 102.0%pH (5%, H
2O, 25°C): 8.7 - 9.3
Insoluble Matter: ≤ 0.005%Chloride (Cl): ≤ 0.001%Nitrogen Compounds (N): ≤ 0.001%Sulfate (SO
4): ≤ 0.005%
Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%Note: While this product is a high quality,
analytical reagent grade buffer, as a good general laboratory practice we recommend filtering solutions before use.
CAS #7782-85-6
20232 100gm 500gm 1kg
Sodium Phosphate, Monobasic(Monosodium Phosphate)
CP GradeNaH
2PO
4
Formula Weight: 119.98PRODUCT SPECIFICATIONSForm: White, slightly hygroscopic crystalline
powderAssay: 98.0 - 103.0% (NaH
2PO
4, dry basis)
Identity: Passes testsArsenic (As): ≤ 3 ppmFluoride (F): ≤ 0.005%Insoluble Substances: ≤ 0.2%Lead (Pb): ≤ 4 ppmLoss on Drying: ≤ 2.0%Note: This product is a general laboratory reagent
suitable for most laboratory animal diets where trace element deficiencies are not required. One molar stock solutions of this product may be slightly hazy. Where use of a high quality research buffer is required, USB recommends the use of our ACS Reagent Grade phosphate buffers.
Storage: Ambient, desiccate (product is slightly hygroscopic).
20229 500gm 1kg 5kg
Sodium Phosphate, Monobasic, Anhydrous
USBioAnalyzedNaH
2PO
4
Formula Weight: 119.98PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 103.0% NaH
2PO
4 (dry basis)
Identity: Passes testpH (5%, H
2O): 4.1 - 4.5
Moisture (KF): ≤ 2.0%Insoluble Substances: ≤ 0.2%Chloride (Cl): ≤ 0.014%Sulfate (SO
4): ≤ 0.15%
Aluminum, Calcium, and Related Elements: Passes test
Arsenic (As): ≤ 8 ppmHeavy Metals (as Pb): ≤ 0.002%O.V.I.: Meets requirementsStorage: Ambient, protect from moisture
32130
Bioc
hem
ical
sBiochemicals
1�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Sodium Phosphate, Monobasic, Monohydrate
CP GradeNaH
2PO
4•H
2O
Formula Weight: 137.98PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 103.0% NaH
2PO
4 (dry basis)
Identity: Passes testsLoss on Drying: 10.0 - 15.0%Arsenic (As): ≤ 3 ppmFluoride (F): ≤ 0.005%Heavy Metals (Pb): ≤ 10 ppmInsoluble Substances: ≤ 0.2%
21664 100gm 500gm 2.5kg
Sodium Phosphate, Monobasic, Monohydrate
ULTRAPUREACS Reagent GradeSlightly HygroscopicNaH
2PO
4•H
2O
Formula Weight: 137.99PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 102.0%pH (5%, H
2O, 25°C): 4.1 - 4.5
Insoluble Matter: ≤ 0.01%Chloride (Cl): ≤ 5 ppmSulfate (SO
4): ≤ 0.003%
Calcium (Ca): ≤ 0.005%Potassium (K): ≤ 0.01%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%CAS #10049-21-5
20233 100gm 500gm 1kg
Sodium Phosphate, TribasicCP GradeNa
3PO
4
Formula Weight: 163.94PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97.0% Na
3PO
4 (ignited basis)
Identification: Passes testsArsenic (As): ≤ 3 ppmFluoride (F): ≤ 0.005%Heavy Metals (as Pb): ≤ 10 ppmInsoluble Substances: ≤ 0.2%Loss on Ignition: ≤ 2.0%
32100
Sodium PropionateSee Propionic Acid, Sodium Salt, page 278
Sodium Pyrophosphate, DecahydrateACS Reagent GradeNa
4P
2O
7•10H
2O
Formula Weight: 446.06PRODUCT SPECIFICATIONSForm: CrystallineAssay: 99.0 - 103.0% (as is)pH (5%, H
2O, 25°C): 9.5 - 10.5
Insoluble Matter: ≤ 0.01%Chloride (Cl): ≤ 0.002%Sulfate (SO
4): ≤ 0.005%
Nitrogen Compounds (N): ≤ 0.001%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%
21601 500gm 2.5kg
Sodium PyruvateSee Pyruvic Acid, Sodium Salt, page 281
Sodium SeleniteNa
2SeO
3
Formula Weight: 172.94CAS #10102-18-8
21665 100gm 500gm
Sodium SuccinateSee Succinic Acid, Disodium Salt, page 291
Sodium Sulfate, AnhydrousACS Reagent GradeNa
2SO
4
Formula Weight: 142.04PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%pH (5%, H
2O, 25°C): 5.2 - 9.2
Insoluble Matter: ≤ 0.01%Loss on Ignition: ≤ 0.5%Chloride (Cl): ≤ 0.001%Nitrogen Compounds (N): ≤ 5 ppmPhosphate (PO
4): ≤ 0.001%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 0.001%Calcium (Ca): ≤ 0.01%Magnesium (Mg): ≤ 0.005%Potassium (K): ≤ 0.01%Extraction-Concentration Suitability: Passes test
21622 500gm 2.5kg
Sodium Sulfite, AnhydrousACS Reagent GradeNa
2SO
3
Formula Weight: 126.04PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0% Insoluble Matter: ≤ 0.005%Free Acid: Passes testTitratable Free Base: ≤ 0.03 meq/gChloride (Cl): ≤ 0.02%Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%
21624 500gm 2.5kg
Sodium TaurocholateSee Taurocholic Acid Sodium Salt, page 294
Sodium ThioglycolateSee Thioglycolic Acid, Sodium Salt, page 297
Sodium Thiosulfate, AnhydrousNa
2S
2O
3
Formula Weight: 158.11PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98% (dry basis)pH (1%, H
2O, 25°C): 6.0 - 8.0
Solubility (1%, H2O): Completely soluble after
15 minutes; clear, colorless solutionInsoluble Matter: ≤ 0.01% Sodium Sulfite (Na
2SO
4): ≤ 0.5%
Sulfide (S): Passes testIron (Fe): ≤ 0.002%Heavy Metals (Pb): ≤ 0.001%Acidity (H
3SO
4): ≤ 0.002%
Alkalinity (NaOH): ≤ 0.01%
21632 100gm 500gm 2.5kg
Biochemicals
Biochemicals
1�5w w w . u s b w e b . c o m
Sodium Thiosulfate, PentahydrateACS Reagent GradeNa
2S
2O
3•5H
2O
Formula Weight: 248.18PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.5 - 101.0% pH (5%, H
2O, 25°C): 6.0 - 8.4
Insoluble Matter: ≤ 0.005%Nitrogen Compounds (N): ≤ 0.002%Sulfate and Sulfite (SO
4): ≤ 0.1%
Sulfide (S): Passes test
21627 500gm 2.5kg
Soluble StarchSee Starch-Soluble, page 290
Sorbic AcidC
6H
8O
2
Formula Weight: 112.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0% (dry basis)Identity: By IRMelting Point: 132 - 135°CMoisture (KF): ≤ 0.5%Arsenic (As): ≤ 3 ppmHeavy Metals (Pb): ≤ 10 ppmResidue on Ignition: ≤ 0.2%CAS #110-44-1
21705 200gm 500gm
D-Sorbitol
ULTRAPUREUSBioAnalyzedC
6H
14O
6
Formula Weight: 182.17PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (dry basis): 91.0 - 100.5%pH (10%, H
2O): 3.5 - 7.0
Identity: By IRIdentity (A, B): Passes testsMicrobial Limits: Passes testMoisture (KF): ≤ 1.5%Residue on Ignition: ≤ 0.1%Reducing Sugars: Passes testLimit of Nickel: Passes testStorage: In tight containers, material is
hygroscopicCAS #50-70-4
21710 500gm 1kg 5kg
Soy Hydrolysate, Animal FreePRODUCT SPECIFICATIONSForm: Tan powderAmino Nitrogen: 2%Total Nitrogen: 7%Loss on Drying: 6%Ash: 15%pH (2%, H
2O): 6.5 – 7.5
Microbial Count: Standard plate count: 5,000 cfu/gm Coliform: 10 cfu/gm
21736 250gm 1kg 5kg
Soy Hydrolysate, Enzymatic(Peptones)
ULTRAPUREPRODUCT SPECIFICATIONSForm: Tan powderProtein (N x 6.25): 55.5 - 59.0%Amino Nitrogen 2.3 - 2.5%Total Nitrogen 8.9 - 9.4%Moisture: 4.0 - 4.9%Ash: 8.0 - 11.0%pH: 6.5 - 7.5Microbial Count: Standard plate count: ≤ 5000/gm Coliform: ≤ 10/gm
21720 1lb 5lb
Soy Hydrolysate, Enzymatic IIPRODUCT SPECIFICATIONSForm: Tan powderAmino Nitrogen: 2.2%Total Nitrogen: 7%Loss on Drying: 6%Ash: 15%pH: 6.5 - 7.5Microbial Count: Standard plate count: 5,000 cfu/gm Coliform: 10 cfu/gm
21718 250gm 1kg 5kg
Soybean Protein(Grade II)
21726 5lb 25lb
Soybean Trypsin Inhibitor(Trypsin Inhibitor)
Source: SoybeanPRODUCT SPECIFICATIONSForm: Tan, lyophilized, salt-free powderActivity: ≥ 7,000 BAEE inhibitor units/mg powderUnit Definition: One unit is that amount which will
inhibit one unit of trypsin activity using N-α-benzoyl-L-arginine ethyl ester (BAEE) as a substrate at 25°C and pH 7.6.
Storage: -20°C, protect from moisture.CAS #9035-81-8
21730 500mg 1gm 5gm
Soybean Trypsin Inhibitor(Trypsin Inhibitor)
Source: SoybeanPRODUCT SPECIFICATIONSForm: Tan, lyophilized, salt-free,
chromatographically purified powderActivity: ≥ 10,000 BAEE inhibitor units/mg
powderUnit Definition: One unit is that amount which
will inhibit one unit of trypsin activity using N-α-benzoyl-L-arginine ethyl ester (BAEE) as a substrate at 25°C and pH 7.6.
Storage: -20°C, protect from moisture.CAS #9035-81-8
21735 100mg 500mg 1gm
Spermidine, Triphosphate[NH
2(CH
2)3NH(CH
2)4NH
2]2•3H
3PO
4•6H
2O
Formula Weight: 692.57Storage: -20°CCAS #49721-50-8
21745 1gm 5gm
Bioc
hem
ical
sBiochemicals
1�6 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Spermidine, Trihydrochloride
ULTRAPUREC
7H
19N
3•3HCl
Formula Weight: 254.63Applications:•Causes a 2-fold increase in full length cDNA with
or without pyrophosphate or Actinomycin D using AMV Reverse Transcriptase
•Used in cosmid cloning•Often included in T4 Polynucleotide Kinase
stocksStorage: -20°CCAS #334-50-9
21760 1gm 5gm
Spermine, Diphosphate SaltNH
2(CH
2)3NH(CH
2)4NH(CH
2)3NH
2•2H
3PO
4
Formula Weight: 398.33Storage: -20°C
21750 1gm
Spermine, TetrahydrochlorideC
10H
26N
4•4HCl
Formula Weight: 348.18Storage: -20°CCAS #306-67-2
21755 1gm 5gm
SSC, 20X Solution
ULTRAPUREMB GradeForm: 20X SSC solution consisting of 0.3M
trisodium citrate and 3.0M sodium chloride in high purity dH
2O, pH 7.0. Solution is 0.2 µm
filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout.
pH (25°C, 20X): 7.0 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testApplication: •Used in nucleic acid blotting and hybridization
techniques
19629 100ml 500ml 1L 5L
SSPE, 20X Solution
ULTRAPUREMB GradeForm: 20X SSPE solution consisting of 3M NaCl,
200mM sodium phosphate and 20mM EDTA in high purity dH
2O, pH 7.4. Solution is 0.2 µm
filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout.
pH (25°C, 20X): 7.4 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75890 1L 5L
Stains-AllC
30H
27N
2S
2Br
Formula Weight: 559.58PRODUCT SPECIFICATIONSForm: Dark blue-green crystalline powderIdentity: By IRDye content: ≥93%Application:•A versatile stain for proteins (red), DNA (blue),
RNA (bluish-purple) and acid polysaccharidesCAS #7423-31-6
32822 1gm 5gm
Starch, Hydrolyzed
ULTRAPURESource: PotatoPRODUCT SPECIFICATIONSForm: White to off-white powderResidue on Ignition: ≤ 1%Loss on Drying: ≤ 20%Gel Strength: Passes testNote: Typically a 12-14% gel is recommended for
electrophoresis. Gel consistency is lot specific and depends on the percentage of amylose and amylopectin in starch, which varies naturally. Optimum concentration must be determined for each lot by the user.
CAS #9005-25-8
32823 100gm 500gm 1kg
Starch-CornCAS #9005-25-8
21805 25lb 100lb
Starch-SolubleACS Reagent GradeSource: PotatoPRODUCT SPECIFICATIONSForm: White powderSolubility: Passes testpH (2%, H
2O, 25°C): 5.0 - 7.0
Loss on Drying: ≤ 12%Sensitivity: Passes testResidue on Ignition: ≤ 0.4%CAS #9005-84-9
21695 500gm 1kg
StaurosporineC
28H
26N
4O
3
Formula Weight: 466.53Form: Non-selective inhibitor of Protein Kinase C.Storage: 4°, in tight containers.CAS #62996-74-1
25115 100µg
Stearic Acid(Octadecanoic Acid)
CH3(CH
2)16
COOHFormula Weight: 284.48PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (GLC): ≥ 99%Storage: -20°CCAS #57-11-4
21835 5gm
Stearic AcidCH
3(CH
2)16
COOHFormula Weight: 284.48CAS #57-11-4
21836 500gm
Biochemicals
Biochemicals
1�7w w w . u s b w e b . c o m
Stock SolutionsAmmonium Acetate (NH
4OAc), 5M Solution,
page 190Betaine, 5M Solution, page 197Dithiothreitol (DTT) 0.1M Solution, page 222DNA Solutions Kit, page 222EDTA, 0.5M Solution, page 225Lithium Chloride (LiCl), 7.5M Solution, page 248Magnesium Chloride (MgCl
2), 1M Solution,
page 250Manganese Chloride (MnCl
2), 1M Solution,
page 253Potassium Acetate (KOAc), 3M Solution, pH 5.5,
page 275Potassium Chloride (KCl), 2M Solution, page 276Protein Determination Reagent, page 279RNA Solutions Kit, page 283Sodium Acetate (NaOAc), 3M Solution, pH 5.5,
page 284Sodium Chloride (NaCl), 5M Solution, page 285Sodium Dodecyl Sulfate (SDS), 10% Solution,
page 286Sodium Dodecyl Sulfate (SDS), 20% Solution,
page 286Water, Nuclease-Free, page 308Water, Nuclease-Free, Endotoxin-Tested,
page 308Water, RNase-Free, DEPC Treated, page 309
Stop Solution
ULTRAPUREContents: 95% Formamide 20mM EDTA 0.05% Bromophenol Blue 0.05% Xylene Cyanol FFApplication: •Used to stop and load sequencing reaction
products onto denaturing acrylamide gelsStorage: -20°C
70724 1200µl
StreptavidinSee Avidin-Streptavidin, page 195
Streptavidin-Alkaline Phosphatase Conjugate
Storage: 4°C
11687 250µl 1,500µl
Streptomycin Sulfate
ULTRAPUREUSBioAnalyzed(C
21H
39N
7O
12)2•3H
2SO
4
Formula Weight: 1457.38PRODUCT SPECIFICATIONSForm: White to off-white powderActivity: 650 µg - 850 µg of C
21H
39N
7O
12/mg (as
is)Identity (A, B): Passes testspH (200 mg streptomycin/ml): 4.5 - 7.0Loss on Drying: ≤ 5.0%Mode of Action:Inhibits protein synthesis by binding to the S12
protein of the 30S ribosomal subunit.CAS #3810-74-0
21865 10gm 25gm 100gm 1kg
StreptozotocinC
8H
15N
3O
7
Formula Weight: 265.22PRODUCT SPECIFICATIONSForm: Off-white to pale yellow crystalline powderAssay: 95.0 - 105.0% (dry basis) 92.0 - 105.0% (as is)Loss on Drying: ≤ 3.0%Residue on Ignition: ≤ 0.3%Heavy Metals (Pb): ≤ 20 ppmStorage: 4°C, in tight containersCAS #18883-66-4
21870 100mg 250mg 500mg 1gm
Succinic Acid, Free AcidHOOCCH
2CH
2COOH
Formula Weight: 118.09PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Identity: By IRMoisture (KF): ≤ 1.0%Iron (Fe): ≤ 5 ppmCAS #110-15-6
21900 250gm 500gm 1kg 5kg
Succinic Acid, Disodium Salt, Hexahydrate(Sodium Succinate)
C4H
4Na
2O
4•6H
2O
Formula Weight: 270.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 97.0 - 102.0% (as is)Identity: By IRLoss on Drying: ≤ 40.0%CAS #6106-21-4
21910 100gm 500gm
Sucrose
ULTRAPUREMB GradeC
12H
22O
11
Formula Weight: 342.30PRODUCT SPECIFICATIONSForm: White/clear crystalline powder[α]25
D : +66.3° to +66.8° (c=26 in H
2O)
A (280 nm, 1M, 1 cm): ≤ 0.125A (260 nm, 1M, 1 cm): ≤ 0.150Insoluble Matter: ≤ 0.005%Loss on Drying: ≤ 0.03%Residue on Ignition: ≤ 0.01%Titratable Acid: ≤ 0.0008 meq/gmInvert Sugar: ≤ 0.05%Chloride (Cl): ≤ 0.005%Sulfate and Sulfite (SO
4): ≤ 0.005%
Arsenic (As): ≤ 1 ppmBarium (Ba): ≤ 1 ppmCadmium (Cd): ≤ 1 ppmCalcium (Ca): ≤ 5 ppmCopper (Cu): ≤ 1 ppmIron (Fe): ≤ 1 ppmLead (Pb): ≤ 1 ppmMagnesium (Mg): ≤ 1 ppmManganese (Mn): ≤ 1 ppmZinc (Zn): ≤ 1 ppmContaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes testCAS #57-50-1
21938 1kg 5kg
Bioc
hem
ical
sBiochemicals
1�8 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
SucroseACS Reagent GradeC
12H
22O
11
Formula Weight: 342.30PRODUCT SPECIFICATIONSForm: White/clear crystalline powder[α]25
D : +66.3° to +66.8° (c=26, H
2O)
Insoluble Matter: ≤ 0.005%Loss on Drying: ≤ 0.03%Residue on Ignition: ≤ 0.01%Titratable Acid ≤ 0.0008 meq/gmChloride (Cl): ≤ 0.005%Sulfate and Sulfite (SO
4): ≤ 0.005%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppmInvert Sugar: ≤ 0.05%
21931 500gm 2.5kg
Sucrose, CrystallineC
12H
22O
11
Formula Weight: 342.30PRODUCT SPECIFICATIONSForm: White/clear crystalsIdentity: By IR[α]20
D : +65.9° to +67.1° (c=26 in H
2O)
Solubility (26%, H2O): Clear and colorless
CAS #57-50-1
21930 1kg 5lb 25lb 100lb
Sucrose OctaacetateUSBioAnalyzedC
28H
38O
19
Formula Weight: 678.59PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 98.0 - 100.5% (dry basis)Melting Point: ≥ 78°CAcidity: Passes testMoisture: ≤ 1.0%Residue on Ignition: ≤ 0.1%CAS #126-14-7
21935 100gm 1kg
Sudan Black BC
29H
24N
6
Formula Weight: 456.54Application•For lipoprotein staining in electrophoresisCAS #4197-25-5
32824 25gm
Sulfadiazine(2-Sulfanilamidopyrimidine)
USBioAnalyzedC
10H
10N
4O
2S
Formula Weight: 250.28PRODUCT SPECIFICATIONSForm: White to slightly yellow crystalline powderAssay: 98.0 - 102.0% (dry basis)Clarity and Color of Solution: Passes testIdentity (A, B, C): Passes testsAcidity: Passes testLoss on Drying: ≤ 0.5%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.002%Selenium (Se): ≤ 0.003%Ordinary Impurities: Passes testCAS #68-35-9
21950 100gm 500gm
Sulfamerazine(2-Sulfanilamido-4-Methyl-Pyrimidine)
C11
H12
N4O
2S
Formula Weight: 264.30PRODUCT SPECIFICATIONSForm: White to faint yellow crystalline powderAssay: 99.0 - 100.5% (dry basis)Identity: By IRMelting Point: 234 - 239°CLoss on Drying: ≤ 0.5%CAS #127-79-7
21960 100gm 1kg
Sulfanilamide(p-Aminobenzene Sulphonamide)
NH2C
6H
4SO
2NH
2
Formula Weight: 172.20CAS #63-74-1
21980 100gm 200gm 1kg
Sulfanilic AcidNH
2C
6H
4SO
3H
Formula Weight: 173.19PRODUCT SPECIFICATIONSForm: White to pale cream colored powderAssay: 98.0 - 102.0% (as is)Identity: By IRCAS #121-57-3
21985 100gm 500gm
Sulfathiazole, Free AcidC
9H
9N
3O
2S
2
Formula Weight: 255.32CAS #72-14-0
21995 100gm 500gm 1kg
Sulfisoxazole(N'-[3,4-Dimethyl-5-Isoxazolyl] Sulfonamide)
C11
H13
N3O
3S
Formula Weight: 267.30PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 99.0 - 101.0% (dry basis)Identity: By IRLoss on Drying: ≤ 0.5%Melting Point: 194 - 199°CCAS #127-69-5
22010 25gm 100gm
Sulfobetaine 8 (SB3-8)(Zwittergent® 3-8; n-Octyl-N,N-Dimethyl-3-Ammonio-1-Propane Sulfonate)
C13
H29
NO3S
Formula Weight: 279.44PRODUCT SPECIFICATIONSForm: White powderAssay (from C): ≥ 98%Identity: By IRSolubility (10%, H
2O): Clear and complete
Conductivity (0.1M, H2O): ≤ 50 µmhos/cm
Moisture (KF): ≤ 2.0%Residue on Ignition: ≤ 0.1%
26210 5gm 25gm 100gm
Sulfobetaine 10 (SB3-10)(Zwittergent® 3-10; n-Decyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate)
C15
H33
NO3S
Formula Weight: 307.49PRODUCT SPECIFICATIONSForm: White powderAssay (from C): ≥ 98%Identity: By IRSolubility (10%, H
2O): Clear and complete
Conductivity (0.1M, H2O): ≤ 50 µmhos/cm
Moisture (KF): ≤ 2.0%Residue on Ignition: ≤ 0.1%
26211 5gm 25gm 100gm
Biochemicals
Biochemicals
1�9w w w . u s b w e b . c o m
Sulfobetaine 12 (SB3-12)(Zwittergent® 3-12; n-Dodecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate)
C17
H37
NO3S
Formula Weight: 335.55PRODUCT SPECIFICATIONSForm: White powderAssay (from C): ≥ 98%Identity: By IRSolubility (10%, H
2O): Clear and complete
Conductivity (0.1M, H2O): ≤ 50 µmhos/cm
Moisture (KF): ≤ 2.0%Residue on Ignition: ≤ 0.1%
26212 5gm 25gm 100gm
Sulfobetaine 14 (SB3-14)(Zwittergent® 3-14; n-Tetradecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate)
C19
H41
NO3S
Formula Weight: 363.60PRODUCT SPECIFICATIONSForm: White powderAssay (from C): ≥ 98%Identity: By IRSolubility (10%, H
2O): Clear and complete
Conductivity (0.1M, H2O): ≤ 50 µmhos/cm
Moisture (KF): ≤ 2.0%Residue on Ignition: ≤ 0.1%
26213 5gm 25gm 100gm
Sulfobetaine 16 (SB3-16)(Zwittergent® 3-16; n-Hexadecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate)
C21
H45
NO3S
Formula Weight: 391.65PRODUCT SPECIFICATIONSForm: White powderAssay (from C): ≥ 98%Identity: By IRSolubility (0.05M, H
2O): Clear and complete
Conductivity (0.05M, H2O): ≤ 50 µmhos/cm
Moisture (KF): ≤ 2.0%Residue on Ignition: ≤ 0.1%
26214 5gm 25gm 100gm
Sulfobetaine 18 (SB3-18)(Zwittergent® 3-18; n-Octadecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate)
C23
H49
NO3S
Formula Weight: 419.71PRODUCT SPECIFICATIONSForm: White powderAssay (from C): ≥ 98%Identity: By IRConductivity (0.05M, H
2O): ≤ 50 µmhos/cm
Moisture (KF): ≤ 2.0%Residue on Ignition: ≤ 0.1%
26215 5gm 25gm 100gm
TAE Buffer, 10X Solution
ULTRAPUREMB GradeForm: 10X TAE solution consisting of 400mM
Tris and 0.01M EDTA in high purity dH2O
adjusted to pH 8.3. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity dH
2O for a solution
consisting of 40mM Tris Acetate, pH 8.3 and 1.0mM EDTA.
pH (25°C, 10X): 8.3 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test
75904 1L 5L
TAE Buffer, 50X Solution
ULTRAPUREForm: 50X TAE solution consisting of 2.0M Tris
and 0.05M EDTA in high purity dH2O and
adjusted to pH 8.3. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity dH
2O for
a solution consisting of 40mM Tris Acetate, pH 8.3 and 1.0mM EDTA.
pH (25°C, 50X): 8.3 ± 0.1
74015 100ml
Tannic Acid(Tannin)
C76
H52
O46
Formula Weight: 1701.20CAS #1401-55-4
22040 200gm 500gm 1kg
TAPS(Tris [Hydroxymethyl] Methyl Aminopropane Sulfonic Acid)
C7H
17NO
6S
Formula Weight: 243.28PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 8.40 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.3Loss on Drying: ≤ 2.0%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #29915-38-6
22043 25gm 100gm 250gm
D-(–)-Tartaric AcidHOOCH(OH)CH(OH)COOHFormula Weight: 150.09CAS #147-71-7
22055 5gm
L-(+)-Tartaric AcidACS Reagent GradeHOOCH(OH)CH(OH)COOHFormula Weight: 150.09PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: ≥ 99.0%Insoluble Matter: ≤ 0.005%Residue on Ignition: ≤ 0.02%Chloride (Cl): ≤ 0.001%Oxalate (C
2O
4): Passes test
Phosphate (PO4): ≤ 0.001%
Sulfur Compounds (SO4): ≤ 0.002%
Heavy Metals (Pb): ≤ 5 ppmIron (Fe): ≤ 5 ppm
22056 500gm 2.5kg
Bioc
hem
ical
sBiochemicals
130 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Taurine(2-Amino Ethane Sulfonic Acid)
C2H
7NO
3S
Formula Weight: 125.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Solubility (2.5%, H
2O): Practically clear and
colorlesspH (5%, H
2O): 4.0 - 7.0
Residue on Ignition: ≤ 0.1%Heavy Metals: ≤ 10 ppmAmmonia (NH
3): ≤ 0.02%
Arsenic (As): ≤ 2 ppmChloride (Cl): ≤ 0.01%Sulfate (SO
4): ≤ 0.01%
CAS #107-35-7
22070 100gm 1kg 5kg
Taurine(2-Amino Ethane Sulfonic Acid)
ULTRAPUREMB GradeC
2H
7NO
3S
Formula Weight: 125.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IRSolubility (0.5M, H
2O): Clear and colorless
pH (5%, H2O): 4.0 - 7.0
A (260 nm, 0.5M, 1 cm): ≤ 0.2A (280 nm, 0.5M, 1 cm): ≤ 0.2Residue on Ignition: ≤ 0.1%Heavy Metals: ≤ 10 ppmAmmonia (NH
3): ≤ 0.02%
Arsenic (As): ≤ 2 ppmChloride (Cl): ≤ 0.01%Sulfate (SO
4): ≤ 0.01%
Use Test: Functionally tested for electrophoresisCAS #107-35-7
75824 50gm 100gm 1kg 5kg
Taurocholic Acid, Sodium Salt(Sodium Taurocholate)
C26
H44
NNaO7S
Formula Weight: 537.68Contains small amounts of Cholic, Glycocholic
and Deoxycholic Acid.CAS #145-42-6
21670 25gm 100gm 500gm
TaxolC
47H
51NO
14
Formula Weight: 853.91Source: Yew BushesPRODUCT SPECIFICATIONSForm: White powderAssay (HPLC): ≥ 97%Storage: -20°C
10119 5mg 10mg
TBE Buffer, 5X Solution
ULTRAPUREMB GradeForm: 5X TBE (Tris-Borate-EDTA) solution
consisting of 0.445M Tris, 0.445M boric acid and 0.01M EDTA. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity water for a solution consisting of 0.089M Tris, 0.089M boric acid and 0.002M EDTA.
Testing: Meets or exceeds functional test criteria for high quality gel electrophoresis.
Application: •Primarily used as a buffer in gel electrophoresis
procedures
75891 1L 5L
TBE Buffer, 10X Ready-Mixed Powder
ULTRAPUREMB GradeForm: Pre-weighed, pre-mixed powdered buffer
packaged in 200 ml bottles for reconstitution to a 10X solution. Reconstitute with high purity dH
2O to 200 ml. After reconstitution, the 10X
solution consists of 0.89M Tris, 0.89M boric acid and 0.02M EDTA. Each pack contains 6 x 200 ml bottles. Total volume is 1.2 liters of 10X buffer or 12 liters of 1X buffer.
Testing: Meets or exceeds functional test criteria for high quality gel electrophoresis.
Application: •Primarily used as a buffer in gel electrophoresis
proceduresStorage: Ambient. The reconstituted buffer
is stable for several days at 4°C if 0.2 µm filtered.
70454 6x200ml
TBS, 20X Solution, pH 7.4
ULTRAPUREMB GradeForm: 20X TBS (Tris Buffered Saline) solution
consisting of 500mM Tris, 60mM KCl and 2.8M NaCl in high purity dH
2O, pH 7.4. Solution is
0.2 µm filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity water for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl and 140mM NaCl.
pH (25°C, 20X): 7.4 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes testApplication: •Used in Western blotting protocols
75892 100ml 1L 5L
Biochemicals
Biochemicals
131w w w . u s b w e b . c o m
TBS with Tween (TBST), 20X Solution, pH 7.4
ULTRAPUREMB GradeForm: 20X TBS (Tris Buffered Saline) solution
consisting of 500mM Tris, 60mM KCl, 2.8M NaCl and 1.0% Tween 20 in high purity dH
2O
adjusted to pH 7.4. Solution is 0.2 µm filtered and is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity water for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween 20.
pH (25°C, 20X): 7.4 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test Protease: Passes testApplication: •Used in Western blotting protocols
77500 1L 5L
TCEP-HCI, 0.5M Solution, pH 6.6 ± 0.1(Tris [Carboxyethyl] Phosphine Hydrochloride)
Form: TCEP-HCI solution consisting of 0.5M TCEP-HCI (Tris(2-Carboxyethyl)Phosphine Hydrochloride) in high purity dH
2O.
pH (25°C): 6.6 ± 0.1Concentration (TCEP): 0.5 - 0.55M
77531 5ml 50ml
TE Buffer, 1X Solution
ULTRAPUREMB GradeForm: 1X TE solution consisting of 10mM Tris, pH
7.5, and 1mM EDTA. Solution is 0.2 µm filtered and dispensed into vials and/or durable, square Nalgene bottles.
pH (25°C): 7.5 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes testApplication: •Use directly to resuspend and/or dilute purified
DNA or RNA
75893 10x1ml 100ml 500ml
TE Buffer, 50X Solution
ULTRAPUREMB GradeForm: 50X TE solution consisting of 500mM Tris,
50mM EDTA in high purity dH2O, adjusted
to pH 7.5. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. Dilute to 1X with high purity dH
2O for a
solution consisting of 10mM Tris, pH 7.5, and 1mM EDTA.
pH (25°C, 50X): 7.5 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
75834 100ml 500ml
TEMEDSee Tetramethylethylenediamine, page 296
Terrific Broth, Ready-Made PowderForm: Off-white free flowing powder.pH (5.08%, H
2O): 7.2 ± 0.2
Contents: Casein Peptone: 12 gm/L Yeast Extract: 24 gm/L Potassium Phosphate Dibasic: 12.5 gm/L Potassium Phosphate Monobasic: 2.3 gm/LApplication: •Provides greater yields of plasmid DNA and
recombinant proteins than LB media by allowing for greater cell density
To use: Add 50.8 gm powder, 4 ml glycerol and 996 ml water to make 1 liter medium. Dissolve and autoclave.
75856 250gm 1kg
TES(N-Tris[Hydroxymethyl]methyl-2-Aminoethanesulfonic Acid)
ULTRAPUREC
6H
15NO
6S
Formula Wt.: 229.25PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity A: KBr pellet matches referenceIdentity B: Passes testpKa (20°C): 7.50 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.2Loss on Drying: ≤ 1.0%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #7365-44-8
22086 25gm 50gm 100gm 500gm 1kg
Tetracycline, Hydrochloride
ULTRAPUREUSBioAnalyzedC
22H
24N
2O
8•HCl
Formula Weight: 480.90PRODUCT SPECIFICATIONSForm: Yellow crystalline powderActivity: ≥ 900 µg of C
22H
24N
2O
8•HCI/mg (as is)
Identity (A, B, C, D, E, F): Passes testspH (10 mg/ml): 1.8 - 2.8[α]25
D : -240° to -255° (c=0.5, 0.1N HCI, dry
basis)Loss on Drying (60°C, 3 hr, vacuum): ≤ 2.0%Limit of 4-Epianhydrotetracycline: ≤ 2.0%Heavy Metals (Pb): ≤ 0.005%Storage: Ambient in tight, light resistant
containers.CAS #64-75-5
22105 25gm 100gm 1kg
n-Tetradecyl-N,N-Dimethyl-3-Ammonio-1-Propane-Sulfonate
See Sulfobetaine 14 (SB3-14), page 293
Bioc
hem
ical
sBiochemicals
13� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
3,3',5,5'-Tetraiodo-L-ThyronineSee L-Thyroxine, page 299
Tetramethylammonium Chloride(TMAC)
ULTRAPUREC
4H
12ClN
Formula Weight: 109.60PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98% (dry basis)Identity: By IRpH (10%, H
2O): ≥ 4.5
Loss on Drying: ≤ 1.5%Solubility (10%, H
2O): Clear and colorless
Applications:•Used in hybridization solutions of mixed
oligonucleotides. In solutions with TMAC, the T
m (melting temperature) of a hybrid
is dependent on its length, not its base composition
•Used in probing PCR products for the analysis of polymorphisms with allele-specific oligonucleotides
Storage: Store at room temperature. This material is extremely hygroscopic.
CAS #75-57-0
22124 500gm 1kg
3,3',5,5'-Tetramethylbenzidine(TMB)
C16
H20
N2
Formula Weight: 240.35PRODUCT SPECIFICATIONSForm: Off-white powderIdentity: By IRMelting Point: 168.3 - 169.9°C
22126 1gm 5gm
3,3',5,5'- Tetramethylbenzidine (TMB) Substrate Solution
ULTRAPUREStorage: 4°C
22128 250ml 1L
N,N,N',N'-Tetramethylethylenediamine(TEMED)
ULTRAPURE(CH
3)2NCH
2CH
2N(CH
3)2
Formula Weight: 116.20PRODUCT SPECIFICATIONSForm: Colorless to pale yellow liquidAssay: ≥ 98%A (290 nm, 1%, 1 cm): ≤ 0.1Density (20°C): 0.77 ± 0.01Refractive Index (20°C): 1.419 ± 0.001Application: •Catalyzes the formation of free radicals by
ammonium persulfate and accelerates the polymerization of acrylamide and bisacrylamide
CAS #110-18-9
76320 100ml 100gm 500gm
Tetranitroblue Tetrazolium Chloride(2,2',5,5'-Tetra-p-Nitropheny1-3,3'- [3,3'-Dimethoxy-4, 4'-Diphenylene]-Ditetrazolium Chloride) ( TNBT )
Completely soluble.C
40H
28N
12O
10Cl
2
Formula Weight: 907.63PRODUCT SPECIFICATIONSForm: Gold powderIdentity: By IRChromatography (TLC): HomogeneousCAS #42798-91-1
22130 250mg 500mg 1gm
Tetrazolium Blue[3,3'- (3,3'-Dimethoxy-4,4'-Biphenylene)-bis- (2,5-Diphenyl)-2H-Tetrazolium Chloride] (BT )
C40
H32
Cl2N
8O
2
Formula Weight: 727.64CAS #1871-22-3
22140 1gm 5gm 10gm 25gm
Tetrazolium RedSee 2,3,5-Triphenyl Tetrazolium Chloride,
page 300
TheophyllineC
7H
8N
4O
2
Formula Weight: 180.16CAS #58-55-9
22165 100gm 1kg
Thiamine, Hydrochloride(Vitamin B
1 ; Aneurine)
USBioAnalyzedC
12H
17CIN
4OS•HCl
Formula Weight: 337.27PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 102.0% (dry basis)Identity (A, B): Passes testsChromatographic Purity: Passes testpH (1%, H
2O): 2.7 - 3.4
Spectral Ratios (400 nm, 10%, 1 cm): ≤ 0.025Moisture (KF): ≤ 5.0%Residue on Ignition: ≤ 0.2%Nitrate: Passes testO.V.I.: Meets the requirementsCAS #67-03-8
22170 25gm 100gm 500gm 1kg
Thiamine, MononitrateC
12H
17N
5O
4S
Formula Weight: 327.36PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.0 - 102.0% (dry basis)Identity: Passes testpH (2%, H
2O): 6.0 - 7.5
Solubility (2%, H2O): Clear and complete
Residue On Ignition: ≤ 0.2%CAS #532-43-4
22175 100gm 1kg
Thiamine Pyrophosphate ChlorideSee Cocarboxylase, page 208
Biochemicals
Biochemicals
133w w w . u s b w e b . c o m
Thiazolyl BlueSee MTT, page 259
ThimerosalUSBioAnalyzedC
9H
9HgNaO
2S
Formula Weight: 404.81PRODUCT SPECIFICATIONSForm: White powderAssay: 97.0 - 101.0% (dry basis)Identity (A, B): Passes testsLoss on Drying: ≤ 0.5%Ether-Soluble Substances: Passes testMercury Ions: ≤ 7%Readily Carbonizable Substances: Passes testCAS #54-64-8
22215 25gm 100gm
Thio-NAD(Thio-Nicotinamide-Adenine-Dinucleotide, Oxidized)
C21
H27
N7O
13SP
2•3H
2O
Formula Weight: 733.5 (trihydrate) 679.5 (anhydrous)Storage: -20°CCAS #4090-29-3
22250 100mg
Thio-NADH(Thio-Nicotinamide-Adenine-Dinucleotide, Reduced)
C21
H27
N7O
13SP
2•Na
2•3H
2O
Formula Weight: 779.52 (trihydrate) 681.51 (anhydrous free acid)PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 90% (enzymatic with ADH, pH 7.5)Spectral Ratios: A
250/A
260: 0.86 ± 0.05 (pH 10)
A280
/A260
: 0.39 ± 0.03 (pH 10)Moisture (KF): ≤ 10%Sodium (Na): 6.0 ± 3.0%Storage: -20°C, desiccated
22252 100mg
Thio-NADP(Thio-Nicotinamide-Adenine-Dinucleotide Phosphate, Oxidized)
C21
H27
N7O
16SP
3•K•4H
2O
Formula Weight: 869.62 (tetrahydrate) 797.56 (anhydrous)PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 93% (enzymatic, pH 7.5)Spectral Ratios: A
250/A
260: 0.89 ± 0.03 (oxidized, pH 7.5)
A280
/A260
: 0.35 ± 0.02 (oxidized, pH 7.5)Moisture (KF): ≤ 8%Potassium: 4.0 ± 2.0%Storage: -20°C, protect from moisture
22253 100mg
ThioacetamideCH
3CSNH
2
Formula Weight: 75.13CAS #62-55-5
22220 25gm 50gm 100gm
Thioglycolic Acid, Sodium Salt(Sodium Thioglycolate; Mercaptoacetic Acid)
HSCH2COONa
Formula Weight: 114.10PRODUCT SPECIFICATIONSForm: White to off-white amorphous powderAssay: ≥ 95.0%Identity: By IRStorage: -20°CCAS #367-51-1
22240 25gm 100gm 500gm
Thioredoxin, Human, Recombinant(r-hTRX)
Formula Weight: 11,734 determined by amino acid sequence
PRODUCT SPECIFICATIONSForm: Lyophilized powder from phosphate bufferSpecific Activity: ≥ 3 units/mg proteinUnit Definition: TRX activity is assayed by
measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5).
Storage: -20°C, protect from moisture
26004 100µg
Thioredoxin, Yeast, Recombinant(r-yTRX)
Formula Weight: 12,600 determined by amino acid sequence
PRODUCT SPECIFICATIONSForm: Lyophilized powder from phosphate bufferSpecific Activity: ≥ 3 units/mg proteinUnit Definition: TRX activity is assayed by
measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5).
Storage: -20°C, protect from moisture
26006 1mg
Thiourea(Thiocarbamide)
NH2CSNH
2
Formula Weight: 76.12PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderIdentity: By IRMelting Point: 171 - 184°CLoss on Drying: ≤ 0.5%CAS #62-56-6
22270 500gm 1kg
D-ThreonineCH
3CH(OH)CH(NH
2)COOH
Formula Weight: 119.12CAS #682-20-2
22280 5gm 25gm 100gm
DL-Threonine(DL-α-Amino-β-Hydroxybutyric Acid)
CH3CH(OH)CH(NH
2)COOH
Formula Weight: 119.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Ammonium (NH
4): ≤ 0.02%
Arsenic (As): ≤ 1 ppmChloride (Cl): ≤ 0.021%Sulfate (SO
4): ≤ 0.028%
CAS #80-68-2
22285 25gm 100gm 1kg
Bioc
hem
ical
sBiochemicals
13� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
L-ThreonineCH
3CH(OH)CH(NH
2)COOH
Formula Weight: 119.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.5%Identity: By IRpH (5%, H
2O): 5.0 - 6.5
[α]25D : -26.7° to -29.1° (c=6 in H
2O)
Loss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.4%Heavy Metals (Pb): ≤ 15 ppmChloride (Cl): ≤ 0.05%Iron (Fe): ≤ 30 ppmSulfate (SO
4): ≤ 0.03%
O.V.I.: Meets requirementsCAS #72-19-5
22290 10gm 25gm 100gm 1kg
Thrombin HumanSource: Human1,000 NIH units/vialPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 1,000 NIH units/vialSpecific Activity: ≥ 2,000 NIH units/mg proteinReconstitution: Reconstitute with 1.0 ml ddH
2O
Buffer: After reconstitution the solution contains: 5mM Sodium Citrate 0.01% PEG 0.02M NaCl pH 6.5All source material tested and found non-reactive
to HTLV-III and HBsAg using FDA approved assay methods.
Storage: -20°C
22292 1000units
Thrombin(E.C.3.4.21.5)Source: Bovine BloodPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity:≥ 40 units/mg powderMoisture: ≤ 5.0%Note: The activity is determined by comparison
to the activity of Standard Thrombin from the Bureau of Biological Standards.
Storage: 4°C, in tight containersCAS #9002-04-4
22293 25,000units 100,000units
Thymidine(Thymidine-2-Deoxyriboside; 2'-Deoxythymidine)
C10
H14
N2O
5
Formula Weight: 242.23PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (UV): 99.0% ± 3Identity: By IRλ
max (pH 7.0): 267 nm ± 1
εmax
(pH 7.0): [9.65 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.65 ± 0.03
A280
/A260
: 0.72 ± 0.03Loss on Drying: ≤ 1.0%Residue on Ignition: ≤ 1.0%Reference Constants:λ
max (pH 7, NRC ): 267 nm
εmax
(pH 7, NRC ): 9.65 x 103
ε260
(pH 7, DBR ): 8.8 x 103
CAS #50-89-5
22305 1gm 5gm 10gm 25gm 100gm
Thymidine-5'-Diphosphate, Sodium Salt
See 2'-Deoxythymidine-5'-Diphosphate, Sodium Salt, page 217
Thymidine-5'-Monophosphate, Disodium Salt
See 2'-Deoxythymidine-5'-Monophosphate, Disodium Salt, page 217
Thymidine-5'-Triphosphate, Trisodium Salt
See 2'-Deoxythymidine-5'-Triphosphate, Trisodium Salt, page 217
Thymidylic AcidSee 2'-Deoxythymidine-5'-Monophosphate,
Disodium Salt, page 217
Thymine(5-Methyluridine)
C5H
6N
2O
2
Formula Weight: 126.11PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Total UV): 98.0% ± 3Chromatography (TLC): HomogeneousIdentity: By IRλ
max (pH 7.0): 264 nm ± 1
εmax
(pH 7.0): [7.9 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.67 ± 0.03
A280
/A260
: 0.53 ± 0.03Reference Constants:λ
max (pH 7, NRC ): 264.5 nm
εmax
(pH 7, NRC ): 7.9 x 103
ε260
(pH 7, DBR ): 7.4 x 103
CAS #65-71-4
22330 10gm 25gm 100gm 1kg
Thymol Blue, Sodium SaltPRODUCT SPECIFICATIONSForm: Dark orange to rust colored powderSolubility (100 mg/100 ml): Clear solutionVisual Transition Interval: Acid Range: pH 2.2 - Pink pH 2.8 - Orange Alkaline Range: pH 8.0 - Yellow pH 8.4 - Green pH 9.2 - BlueCAS #62625-21-2
22340 5gm 10gm
ThymolphthaleinC
28H
30O
4
Formula Weight: 430.54pH range: 8.8 (colorless) - 10.5 (blue)CAS #125-20-2
22350 25gm 100gm
ThyronineSee Triiodo-L-Thyronine, page 300
Biochemicals
Biochemicals
135w w w . u s b w e b . c o m
DL-Thyroxine, Sodium SaltC
15H
10I4NNaO
4
Formula Weight: 798.83CAS #1491-91-4
22390 500mg 1gm 5gm
L-ThyroxineC
15H
11I4NO
4
Formula Weight: 776.88CAS #51-48-9
22394 250mg 1gm
L-Thyroxine, Sodium Salt(3,3',5,5'-Tetraiodo-L-Thyronine)
C15
H10
I4NNaO
4•5H
2O
Formula Weight: 888.88PRODUCT SPECIFICATIONSForm: White to slightly brownish-yellow powderAssay: 97.0 - 101.0% (dry basis)Identity: By IR[α]25
D : -5.0° to -6.0° (c=3 in 1N NaOH:ETOH 1:2)
Loss on Drying: ≤ 11.0%Tri-iodothyronine (HPLC): ≤ 2.0% (dry basis)CAS #6106-07-6
22395 500mg 1gm 5gm
TMACSee Tetramethylammonium Chloride, page 296
TMBSee 3,3',5,5'-Tetramethylbenzidine, page 296
TNBTSee Tetranitroblue Tetrazolium Chloride, page 296
D-α-Tocopherol(Vitamin E)
C29
H50
O2
Formula Weight: 430.71CAS #59-02-9
22410 25gm 100gm
DL- -Tocopherol(Vitamin E)
C29
H50
O2
Formula Weight: 430.71PRODUCT SPECIFICATIONSForm: Yellow to amber, clear viscous oilAssay: 96.0 - 102.0%Identity: Passes testAcidity: Passes testHeavy Metals (Pb): ≤ 0.004%Storage: Ambient, protect from lightCAS #10191-41-0
22415 100gm 1kg
DL-α-Tocopherol Acetate(Vitamin E Acetate)
C31
H52
O3
Formula Weight: 472.74CAS #7695-91-2
22425 25gm 100gm 1kg
DL-α-Tocopheryl AcetateC
31H
52O
3
Formula Weight: 472.74PRODUCT SPECIFICATIONSForm: White to off-white free-flowing powder
standardized with cornstarchPotency: 250 IU/gm powderCAS #7695-91-2
22420 25gm 100gm 500gm 1kg
α-Tocopherol QuinoneC
29H
50O
3
Formula Weight: 446.71Storage: 4°CCAS #7559-04-8
22405 1gm
p-Tosylamido-2-Phenylethyl Chloromethyl Ketone(TPCK)
C17
H18
ClNO3S
Formula Weight: 351.85CAS #402-71-1
22486 250mg 1gm
N -p-Tosyl-L-Lysine Chloromethyl Ketone, Hydrochloride(TLCK)
C14
H21
ClN2O
3S•HCl
Formula Weight: 369.31A Papain and Trypsin inhibitorStorage: -20°CCAS #4272-74-6
22480 100mg 500mg 1gm
Toxillic AcidSee Maleic Acid, page 252
Transferrin, HoloIron saturatedSource: Human BloodPRODUCT SPECIFICATIONSForm: Pink lyophilized powderAssay: ≥ 98.0%Protein: ≥ 98.0%Moisture: ≤ 5.0%Iron content: 1.2 - 1.7 µg/mgStorage: 4°C
22508 100mg
Transferrin, APO HumanSource: Human PlasmaPRODUCT SPECIFICATIONSForm: White lyophilized powderpH (1%:): 6.5 - 8.0Protein (Biuret): ≥ 95.0%Assay (electrophoresis): ≥ 98.0%Iron Content: ≤ 0.03 mg/gm proteinIron Binding Capacity: ≥ 1.0 mg/gm proteinPurity: ≥ 98%Storage: 4°CCAS #11096-37-0
22517 100mg 500mg 1gm
Transferrin, Pooled PlasmaSource: Bovine BloodStorage: 4°C
22511 100mg 500mg
Bioc
hem
ical
sBiochemicals
136 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
, -Trehalose, Dihydrate(α-D-Glucopyranosyl-α-D-Glucopyranoside)
C12
H22
O11
•2H2O
Formula Weight: 378.33PRODUCT SPECIFICATIONSForm: White crystalline powderMoisture (KF): 9.3 - 9.7%[α]20
D : +179.0° ± 1.5° (c=7, H
2O)
Arsenic (As): ≤ 0.5 ppmIron (Fe): ≤ 5 ppmHeavy Metals (Cu): ≤ 10 ppmCAS #6138-23-4
22515 10gm 100gm
Tricine[N-Tris (Hydroxymethyl)methylglycine]
ULTRAPURE(CH
2OH)
3CNHCH
2COOH
Formula Weight: 179.17PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 8.15 ± 0.15A (250 nm, 5%, 1 cm): ≤ 0.1Loss on Drying: ≤ 0.5%Iron (Fe): ≤ 30 ppmLead (Pb): ≤ 10 ppmCAS #5704-04-1
22561 25gm 100gm 500gm 1kg
TriethanolamineN(CH
2CH
2OH)
3
Formula Weight: 149.19PRODUCT SPECIFICATIONSForm: Clear, viscous liquidAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 7.85 ± 0.15Moisture: ≤ 0.5%Diethanolamine: ≤ 0.9%Ethanolamine: ≤ 0.1%Iron (Fe): ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmStorage: Ambient, in tight, light-resistant
containers, under inert gas.CAS #102-71-6
22564 500ml 1L
Triethanolamine, HydrochlorideN(CH
2CH
2OH)
3•HCl
Formula Weight: 185.65PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: ≥ 98.5% (dry basis)Identity: By IRpKa (20°C): 7.85 ± 0.15Melting Point: 178 - 182°CLoss on Drying: ≤ 1%Iron (Fe): ≤ 30 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #637-39-8
22565 100gm 250gm 1kg
1,3,5-TrihydroxybenzeneSee Phloroglucinol, page 272
3,3',5-Triiodo-L-Thyronine, Free Acid(Thyronine)
C15
H12
I3NO
4
Formula Weight: 650.96CAS #6893-02-3
22590 100mg 500mg 1gm 5gm 10gm
3,3',5-Triiodo-L-Thyronine, Sodium Salt
C15
H11
NNaO4I3
Formula Weight: 672.96PRODUCT SPECIFICATIONSForm: Tan powderAssay (HPLC): ≥ 95%Identity: By IRMoisture (LOD): ≤ 5%CAS #55-06-1
22591 100mg 500mg 1gm
1,3,7-TrimethylxanthineSee Caffeine, page 201
2,6,8-TrioxypurineSee Uric Acid, page 306
Tripalmitin(Glyceryl Tripalmitate)
C51
H98
O6
Formula Weight: 807.32
22625 50gm 100gm
2,3,5-Triphenyl Tetrazolium Chloride(TTC; Tetrazolium Red)
C19
H15
ClN4
Formula Weight: 334.80PRODUCT SPECIFICATIONSForm: Cream to beige colored powderIdentity: By IRSolubility (1%, H
2O): Clear solution
Moisture: ≤ 5% (By KF)Sensitivity for Reducing Sugars: Passes testCAS #298-96-4
22631 5gm 10gm 25gm 50gm 100gm 500gm 1kg
Triphosphopyridine Nucleotide Dinucleotide
See Nicotinamide Adenine Dinucleotide, page 262See Nicotinamide Adenine Dinucleotide Reduced,
page 262
2,4,6-Tripyridyl-s-Triazine (TPTZ)C
18H
12N
6
Formula Weight: 312.33CAS #3682-35-7
22660 1gm 5gm 25gm 100gm
Biochemicals
Biochemicals
137w w w . u s b w e b . c o m
Tris(Tris[hydroxymethyl]aminomethane)
ACS Reagent GradeNH
2C(CH
2OH)
3
Formula Weight: 121.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.8 - 100.1% (dry basis)A (290 nm, 40%, 1 cm): ≤ 0.2Moisture (KF): ≤ 2%Insoluble Matter: ≤ 0.005%Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 5 ppm
22664 500gm 2.5kg
Tris(Tris[hydroxymethyl]aminomethane)
ULTRAPUREMB GradeNH
2C(CH
2OH)
3
Formula Weight: 121.14PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.8 - 100.1% (dry basis)Identity: By IRpKa (20°C): 8.30 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.1A (290 nm, 40%, 1 cm): ≤ 0.2Solubility (1M, 500 ml): Clear and colorlesspH (5%, H
2O): 10.0 - 11.5
Melting Point: 168 - 172°CLoss on Drying: ≤ 1%Moisture (KF): ≤ 1%Residue on Ignition: ≤ 0.1%Insoluble Matter: ≤ 0.005%Arsenic (As): ≤ 1 ppmBarium (Ba): ≤ 1 ppmCadmium (Cd): ≤ 1 ppmCalcium (Ca): ≤ 1 ppmCopper (Cu): ≤ 1 ppmIron (Fe): ≤ 1 ppmLead (Pb): ≤ 1 ppmMagnesium (Mg): ≤ 1 ppmManganese (Mn): ≤ 1 ppmZinc (Zn): ≤ 1 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testProtease: Passes testCAS #77-86-1
75825 500gm 1kg 5kg 10kg
Tris[Tris(hydroxymethyl)aminomethane]
High Purity GradeNH
2C(CH
2OH)
3
Formula Weight: 121.14PRODUCT SPECIFICATIONSForm: White/clear crystalline powderAssay: 99.0 - 101.0% (dry basis)Identity: By IRpKa (20°C): 8.30 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.1A (290 nm, 40%, 1 cm): ≤ 0.2pH (5%, H
2O): 10.0 - 11.5
Melting Point: 168 - 178°CLoss on Drying: ≤ 1%Residue on Ignition: ≤ 0.1%Insoluble Matter: ≤ 0.005%Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 5 ppmCAS #77-86-1
22674 250gm 500gm 1kg 5kg
Tris[Tris(hydroxymethyl)aminomethane]
Reagent GradeNH
2C(CH
2OH)
3
Formula Weight: 121.14PRODUCT SPECIFICATIONSForm: White to pale yellow crystalline powderAssay: ≥ 98.0% (dry basis)Identity: By IRpH (5%, H
2O): 10.0 - 11.5
Melting Point: ≥ 160°CLoss on Drying: ≤ 1.5%CAS #77-86-1
22675 250gm 500gm 1kg
Tris, Acetate(Tris (Hydroxymethyl) Amino Methane Acetate)
Reagent GradeNH
2C(CH
2OH)
3CH
3COOH
Formula Weight: 181.19PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRA (250 nm, 5%, 1 cm): ≤ 0.3Moisture (KF): ≤ 1.0%CAS #6850-28-8
22671 25gm 100gm 250gm
Tris (Carboxyethyl) Phosphine, Hydrochloride(TCEP)
C9H
15O
6P•HCl
Formula Weight: 286.65Storage: 4°C
22636 5gm 10gm
Tris-Glycine (TG) Buffer, 10X Solution
ULTRAPUREMB GradeForm: 10X Tris-Glycine (TG) Buffer consisting
of 0.25M Ultrapure Tris, pH 8.3, and 1.92M glycine. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity water and methanol for a solution consisting of 25mM Tris, pH 8.3, 192mM glycine, 20% methanol.
pH (25°C, 10X): 8.3 ± 0.1Contaminant Testing: Protease: Passes test.Application: •For Western blotting and gel electrophoresis
75894 1L 5L
Tris-Glycine-SDS (TGS) Buffer, 10X Solution
ULTRAPUREMB GradeForm: 10X Tris-Glycine-SDS (TGS) Buffer,
consisting of 0.25M Ultrapure Tris, pH 8.6, 1.92M glycine and 1.0% SDS. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout. Dilute to 1X with high purity dH
2O for a solution consisting of 25mM Tris,
pH 8.6, 192mM glycine and 0.1% SDS.pH (25°C, 10X): 8.3 ± 0.1Contaminant Testing: Protease: Passes testApplication: •TGS is the most commonly used buffer for SDS-
PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) of proteins
75895 1L 5L
Bioc
hem
ical
sBiochemicals
138 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Tris, Hydrochloride, GranularMB GradeC
4H
11NO
3•HCl
Formula Weight: 157.60PRODUCT SPECIFICATIONSForm: White granulesAssay: 99 - 101.0% (as is)Identity: By IRpKa (20°C): 8.30 ± 0.15Spectral Ratios (250 nm, 1 M, 1 cm): ≤ 0.2Spectral Ratios (290 nm, 40%, 1 cm): ≤ 0.2pH (5%, H
2O): 4.7 ± 0.5
Melting Point: 148 - 157°CLoss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.1%Insoluble Matter: ≤ 0.005%Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 5 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testProtease: Passes test
22665 1kg
Tris, Hydrochloride(Tris[hydroxymethyl]aminomethane Hydrochloride)
ULTRAPUREMB GradeC
4H
11NO
3 HCl
Formula Weight: 157.60PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 101.0% (as is)Identity: By IRpKa (20°C): 8.30 ± 0.15A (250 nm, 1M, 1 cm): ≤ 0.20A (290 nm, 40%, 1 cm): ≤ 0.20pH (5%, H
2O): 4.7 ± 0.5
Melting Point: 148 - 157°CLoss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.1%Insoluble Matter: ≤ 0.005%Iron (Fe): ≤ 5 ppmHeavy Metals (Pb): ≤ 5 ppmDNase (endo): Passes testDNase (exo): Passes testRNase: Passes testProtease: Passes testCAS #1185-53-1
22676 100gm 250gm 500gm 1kg 5kg
Tris/HCl, 1M Solution, pH 7.0
ULTRAPUREMB GradeForm: 1M solution of Tris in high purity dH
2O,
adjusted to pH 7.0. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles.
pH (25°C): 7.0 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
22637 100ml 500ml 1L
Tris/HCl, 1M Solution, pH 7.5
ULTRAPUREMB GradeForm: 1M solution of Tris in high purity dH
2O,
adjusted to pH 7.5. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles.
pH (25°C): 7.5 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
22639 100ml 500ml 1L
Tris/HCl, 1M Solution, pH 8.0
ULTRAPUREMB GradeForm: 1M solution of Tris, Ultrapure in high purity
dH2O, adjusted to pH 8.0. Solution is 0.2 µm
filtered and dispensed into durable, square Nalgene bottles.
pH (25°C): 8.0 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes testStorage: Prior to opening may be stored at
ambient temperature. After opening, store at 4°C.
22638 100ml 500ml 1L
Tris, 2M Solution
ULTRAPUREMB GradeForm: 2M solution of Tris in high purity dH
2O.
Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles.
Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
22678 100ml 500ml
Tris, 2M Solution, pH 7.5
ULTRAPUREMB GradeForm: 2M solution of Tris, Ultrapure (MW 121.14)
in deionized water adjusted to pH 7.5 with HCl. Solution is 0.2 µm filtered and dispensed into durable, square Nalgene bottles.
pH (25°C): 7.5 ± 0.1Contaminant Testing: DNase (endo): Passes test DNase (exo): Passes test RNase: Passes test Protease: Passes test
77514 100ml 1L
Tris, Phosphate, MonobasicReagent GradeNH
2C(CH
2OH)
3•H
3PO
4
Formula Weight: 219.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98%Identity: By IRA (280 nm, 5%, 1 cm): ≤ 0.05Solubility (5%, H
2O): Clear and colorless
Loss on Drying: ≤ 3.0%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 5 ppmCAS #6992-39-8
22684 25gm 100gm 250gm
Biochemicals
Biochemicals
139w w w . u s b w e b . c o m
Tris, Sulfate(NH
2C(CH
2OH)
3)2•H
2SO
4
Formula Weight: 340.35CAS #6992-38-7
22688 25gm 100gm 250gm
Triticum Vulgaris Agglutinin(Wheat Germ Agglutinin)
PRODUCT SPECIFICATIONSForm: White to slightly off-white lyophilized
powderAssay: Single band by disc gel electrophoresis;
7.5% poly-acrylamide gel, Tris-glycine buffer pH 8.8, 50 µg sample.
Specificity: N-acetylglucosamine, Sialic acid.Activity: Less than 4 mg/ml will agglutinate
human type 0 erythrocytes. Less than 1 µg/ml will agglutimate neuraminidase treated erythrocytes.
Other Data: Salt and carbohydrate free; Non-mitogenic
Reconstitute with 0.01M Phosphate buffer. Do not inhale lectin or allow skin contactStorage: -20°C, desiccated
23453 5mg 25mg 100mg
Triton® X-100
ULTRAPUREPRODUCT SPECIFICATIONSForm: Clear liquidpH (5%, H
2O): 6.0 - 8.0
Cloud point: 63 - 69°C for 1% aqueous solutionColor, APHA: ≤ 100CAS #9002-93-1
22686 500ml 1ga
r-hTRX, r-yTRXSee Thioredoxin, Recombinant Human, Yeast,
page 297
Trypsin
ULTRAPURE(E.C.3.4.21.4)USBioAnalyzedSource: Bovine PancreasPRODUCT SPECIFICATIONSForm: White to pale yellow powderActivity: ≥ 2,500 USP units/mg powderContaminating Activity: Chymotrypsin: ≤ 50 USP units/mg powderLoss on Drying: ≤ 5.0%Solubility: Passes testResidue on Ignition: ≤ 2.5%Microbial Limits Test per USP: Passes testStorage: -20°C, protect from moisture.CAS #9002-07-7
22720 1gm 5gm 10gm
Trypsin (TPCK Treated)(E.C.3.4.21.4)Source: Bovine PancreasPRODUCT SPECIFICATIONSForm: Lyophilized powder, treated with TPCK to
inhibit chymotryptic activityActivity: ≥ 180 units/mg proteinUnit Definition: One unit hydrolyzes 1 µmol of
TAME/min at 25°C, pH 8.2, in the presence of 0.01M Ca2+.
Storage: 4°C
22725 100mg 250mg
Trypsin 1:100(E.C.3.4.21.4)Source: Porcine PancreasPRODUCT SPECIFICATIONSForm: Powder stabilized with lactoseActivity: Trypsin: ≥ 100,000 USP units/gm Chymotrypsin ≥ 30,000 USP units/gmUnit Definition: One unit causes a change in
absorbance of 0.003/min with BAEE as a substrate at 25°C, pH 7.6.
Storage: 4°CCAS #9002-07-7
22705 100gm 500gm 1kg
Trypsin 1:250(E.C.3.4.21.4)Source: Porcine PancreasPRODUCT SPECIFICATIONSForm: Powder stabilized with lactose.Activity: ≥ 250,000 USP units/gmChymotrypsin: ≥ 75,000 USP units/gmUnit Definition: One unit causes a change in
absorbance of 0.003/min with BAEE as a substrate at 25°C, pH 7.6.
Storage: 4°C
22710 25gm 100gm 1kg
Trypsin 1:300(E.C.3.4.21.4)Source: Porcine PancreasPRODUCT SPECIFICATIONSForm: Powder stabilized with lactoseTrypsin Activity: ≥ 300,000 USP units/gm (as is)Chymotrypsin Activity: ≥ 90,000 USP units/gm
(as is)Loss on Drying: ≤ 5.0%Unit Definition: One unit causes a change in
absorbance of 0.003/min with BAEE as a substrate at 25°C, pH 7.6.
Storage: 4°C
22715 25gm 100gm 1kg
Trypsin InhibitorsSee Lima Bean Trypsin Inhibitor, page 248See Soybean Trypsin Inhibitor, page 289
TrypsinogenSource: Bovine PancreasPRODUCT SPECIFICATIONSForm: Crystallized, contains minimal tryptic
activityActivity: Activates to 150 to 200 units/mgUnit Definition: One unit hydrolyzes 1 µmol of
TAME/min at 25°C, pH 8.2, in the presence of ionized calcium.
Storage: 4°C
22745 100mg 500mg 1gm
Bioc
hem
ical
sBiochemicals
1�0 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
D-Tryptophan(C
8H
6N)CH
2CH(NH
2)COOH
Formula Weight: 204.23CAS #153-94-6
22755 25gm 100gm
L-Tryptophan(L-2-Amino-3-Indole Propionic Acid)
USBioAnalyzedC
11H
12N
2O
2
Formula Weight: 204.23PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 98.5 - 101.5% C
11H
12N
2O
2 (dry basis)
Identity: By IRpH (1%, H
2O): 5.5 - 7.0
Chromatography (TLC): Homogeneous[α]25
D : -29.4° to -32.8° (c=1 in H
2O)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Heavy Metals (Pb): ≤ 0.0015%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Sulfate (SO
4): ≤ 0.03%
O.V.I.: Meets the requirementsCAS #73-22-3
22765 25gm 100gm 1kg
L-Tryptophan-β-NapthylamideC
21H
19N
3O
Formula Weight: 329.40PRODUCT SPECIFICATIONSForm: Off-white powderChromatography (TLC): HomogeneousIdentity: By IRStorage: 4°C
22791 100mg 500mg 1gm
Tween® 20(Polyoxyethylene Sorbitan Monolaurate)
ULTRAPUREPRODUCT SPECIFICATIONSForm: Yellow liquidIdentity: Passes testSaponification Number: 40 - 50 mg KOH/gHydroxyl Number: 96 - 108 mg KOH/gAcid Number: ≤ 2.2 mg KOH/gWater: ≤ 3.0%Specific Gravity: 1.1 g/ml (approx.) (25°C)Viscosity: 370 cps (approx.) (25°C)Solubility (10%, H
2O): Soluble
CAS #9005-64-5
20605 500ml 1ga
Tween® 40(Polyoxyethylene Sorbitan Monolaurate)
PRODUCT SPECIFICATIONSForm: Yellow liquidIdentity: Passes testsSaponification Number: 41 - 52 mg KOH/gHydroxyl Number: 89 - 105 mg KOH/gAcid Number: ≤ 2.2 mg KOH/gWater: ≤ 3.0%CAS #9005-66-7
20606 1ga
Tween® 80(Polyoxyethylene Sorbitan Monooleate)
USBioAnalyzedPRODUCT SPECIFICATIONSForm: Yellow viscous liquidIdentity (A, B, C): Passes testsSpecific Gravity (25°C): 1.06 - 1.09Viscosity (25°C): 300 - 500 cpsWater: ≤ 3.0%Residue on Ignition: ≤ 0.25%Heavy Metals (Pb): ≤ 0.001%Acid Value: ≤ 2.2Hydroxy Value: 65 - 80Saponification Value: 45 - 55Residual Solvents: Passes testCAS #9005-65-6
20608 500ml 1ga
Tyramine, Hydrochloride(Tyrosamine)
HO(C6H
4)CH
2CH
2NH
2•HCl
Formula Weight: 173.64CAS #60-19-5
22875 2gm 5gm 25gm 100gm
TyrosamineSee Tyramine, page 304
Tyrosinase(Polyphenol Oxidase)
(E.C.1.14.18.1)Source: MushroomStorage: -20°C
22885 100,000units 500,000units
D-TyrosineHO(C
6H
4)CH
2CH(NH
2)COOH
Formula Weight: 181.19CAS #556-02-5
22890 1gm 5gm
DL-TyrosineHO(C
6H
4)CH
2CH(NH
2)COOH
Formula Weight: 181.19CAS #556-03-6
22895 25gm 100gm
Biochemicals
Biochemicals
1�1w w w . u s b w e b . c o m
L-TyrosineUSBioAnalyzedHO(C
6H
4)CH
2CH(NH
2)COOH
Formula Weight: 181.19PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IR[α]25
D : -9.8° to -11.2° (dry basis, c=5 in 1N HCl)
Loss on Drying (105°C, 3 hr): ≤ 0.3%Residue on Ignition: ≤ 0.4%Chloride (Cl): ≤ 0.04%Iron (Fe): ≤ 30 ppmSulfate (SO
4): ≤ 0.04%
Heavy Metals (Pb): ≤ 15 ppmChromotographic Purity: Passes testO.V.I.: Meets the requirementsCAS #60-18-4
22910 100gm 200gm 1kg 5kg
DL-o-TyrosineC
9H
11NO
3
Formula Weight: 181.19CAS #2370-61-8
22905 500mg 1gm
L-Tyrosine, Disodium SaltC
9H
9NO
3Na
2•nH
2O
Formula Weight: 225.15•nH2O
PRODUCT SPECIFICATIONSForm: Pale beige to light tan powderAssay: ≥ 98.0% (dry basis)Identity: By IRMoisture (KF): ≤ 20%pH (10%, H
2O): 11.5 ± 0.5
Storage: Ambient, in tightly closed containers (material is very hygroscopic)
CAS #122666-87-9
22927 25gm 100gm 1kg 5kg
L-Tyrosine, HydrochlorideHO(C
6H
4)CH
2CH(NH
2)COOH•HCl
Formula Weight: 217.65PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 98.0%Identity: By IRSolubility (4%, 1N HCl): Clear and complete[α]20
D : -9.4° to -10.2° (c=5 in 1N HCl)
Loss on Drying (110°C): ≤ 1.0%Heavy Metals (Pb): ≤ 20 ppmCAS #16870-43-2
22935 1gm 5gm 10gm
UracilC
4H
4N
2O
2
Formula Weight: 112.09PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (Total UV, pH 7.0): ≥ 98.0% (dry basis)Identity: By IRSpectral Ratios (pH 7.0): A
280/A
260: 0.18 ± 0.02
A250
/A260
: 0.84 ± 0.03Loss on Drying (105°C, 2 hr): ≤ 5.0%Residue on Ignition: ≤ 0.1%Reference Constants:λ
max (pH 7, NRC ): 260 nm
εmax
(pH 7, NRC ): 8.2 x 103
ε260
(pH 7, DBR ): 8.2 x 103
CAS #66-22-8
23020 100gm 1kg
UreaACS Reagent GradeNH
2CONH
2
Formula Weight: 60.06PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5%Melting Point: 132 - 135°CInsoluble Matter: ≤ 0.01%Residue on Ignition: ≤ 0.01%Chloride (Cl): ≤ 5 ppmSulfate (SO
4): ≤ 0.001%
Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%
23041 500gm 2.5kg
Urea
ULTRAPUREMB GradeNH
2CONH
2
Formula Weight: 60.06PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.5%Identity: By IRSpectral Ratios (260 nm, 6M, 1 cm): ≤ 0.055Spectral Ratios (280 nm, 6M, 1 cm): ≤ 0.044Melting Point: 132 - 135°CInsoluble Matter ≤ 0.01%Residue on Ignition: ≤ 0.01%Conductivity: ≤ 15 µmhos/cmCyanate: None detectedChloride (Cl): ≤ 0.0005%Sulfate (SO
4): ≤ 0.001%
Copper (Cu): ≤ 0.5 ppmIron (Fe): ≤ 0.5 ppmLead (Pb): ≤ 0.5 ppmDNase (endo): Passes testRNase: Passes testProtease: Passes testApplications:•Chaotropic agent used in denaturing
polyacrylamide gel electrophoresis•When 8M urea is not sufficiently denaturing,
40% formamide may be includedCAS #57-13-6
75826 1kg 5kg
UreaHigh Purity GradeNH
2CONH
2
Formula Weight: 60.06PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 100.5%Loss on Drying: ≤ 1.0%Melting Point: 132 - 135°CInsoluble Matter: ≤ 0.01%Residue on Ignition: ≤ 0.01%Chloride (Cl): ≤ 5 ppmSulfate (SO
4): ≤ 0.001%
Heavy Metals (Pb): ≤ 0.001%Iron (Fe): ≤ 0.001%CAS #57-13-6
23036 500gm 1kg 5kg
Bioc
hem
ical
sBiochemicals
1�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
r-Urease, Recombinant(3.5.1.5)Source: E.coliPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity: ≥ 150 units/mg proteinProtein (per mg of powder): 500 ± 300 µgPotassium Phosphate (per mg of powder): 470
± 300 µgEDTA Na
2 (per mg of powder): 30 ± 10 µg
Contaminating Activity: NADPH Oxidase: ≤ 0.001%Storage: -20°C, protect from moisture
26117 1,000units
Uric Acid(2,6,8-Trioxypurine)
C5H
4N
4O
3
Formula Weight: 168.11PRODUCT SPECIFICATIONSForm: White to off-white powderAssay: ≥ 98.5%Identity: By IRSolubility: Clear solutionLoss on Drying: ≤ 0.2%Residue on Ignition: ≤ 0.2%Heavy Metals: ≤ 20 ppmCAS #69-93-2
23075 10gm 25gm 100gm 500gm 1kg
Uricase(E.C.1.7.3.3)Source: Yeast, Candida utilisPRODUCT SPECIFICATIONSForm: Lyophilized powderActivity ≥ 6 units/mg proteinUnit Definition: One unit hydrolyzes 1 µmole of
uric acid/min at 25°C, pH 8.5.Buffer: Na
2B
4O
7•10H
2O
Note: The above product is available in manufacturing quantities. Please inquire for bulk pricing.
Storage: -20°CCAS #9002-12-4
23089 10units 50units 100units 500units 1,000units
UridineC
9H
12N
2O
6
Formula Weight: 244.20PRODUCT SPECIFICATIONSForm: White to slightly off-white powderAssay (UV): 100% ± 2%Identity: By IRλ
max (pH 7.0): 262 nm ± 1 nm
εmax
(pH 7.0): [10.1 ± 2%] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.74 ± 0.03
A280
/A260
: 0.36 ± 0.02Loss on Drying: ≤ 1.0%Heavy Metals (Pb): ≤ 10 ppmReference Constants:λ
max (pH 7, NRC ): 262 nm
εmax
(pH 7, NRC ): 10.1 x 103
ε260
(pH 7, DBR ): 10.0 x 103
CAS #58-96-8
23130 1gm 5gm 10gm 100gm
Uridine-5'-Diphosphate, Disodium SaltC
9H
12N
2O
12P
2Na
2•nH
2O
Formula Weight: 448.12•nH2O
Reference Constants:λ
max (pH 7, NRC ): 262 nm
εmax
(pH 7, NRC ): 10.0 x 103
ε260
(pH 7, DBR ): 9.9 x 103
Storage: -20°C, protect from moistureCAS #2781-45-0
23135 25mg 100mg 500mg 1gm
Uridine-5'-Diphospho-Glucose, Disodium Salt
C15
H22
N2O
17P
2Na
2•2H
2O
Formula Weight: 610.27 (anhydrous) 646.30 (dihydrate) Storage: 4°CCAS #28053-08-9
23140 500mg 1gm
Uridine-5'-Monophosphate, Disodium Salt(UMP; Uridine Monophosphate)
C9H
11N
2O
9PNa
2•nH
2O
Formula Weight: 368.14•nH2O
PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: (Total UV): ≥ 96.0% (dry basis)λ
max (pH 7.0): 262 nm ± 2 nm
εmax
(pH 7.0): [10.0 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.73 ± 0.03
A280
/A260
: 0.39 ± 0.02Reference Constants:λ
max (pH 7, NRC ): 262 nm
εmax
(pH 7, NRC ): 10.0 x 103
ε260
(pH 7, DBR ): 9.9 x 103
Storage: 4°CCAS #3387-36-8
23155 5gm
Uridine-5'-Triphosphate, Sodium Salt, 100mM Solution(UTP)
ULTRAPUREC
9H
11N
2O
15P
3Na
4
Formula Weight: 572.07Form: 100mM aqueous solution, pH 7.5Storage: -20°C
77244 25µmol(250µl)
Uridine-5'-Triphosphate, Trisodium Salt(UTP)
C9H
12N
2O
15P
3Na
3•2H
2O
Formula Weight: 586.12PRODUCT SPECIFICATIONSForm: White crystalline powderAssay (Total UV): 100% ± 3% (dry basis)λ
max (pH 7.0): 262 nm ± 1 nm
εmax
(pH 7.0): [10.0 ± 0.3] x 103
Spectral Ratios (pH 7.0): A
250/A
260: 0.75 ± 0.03
A280
/A260
: 0.38 ± 0.02Moisture: ≤ 15.0%Reference Constants:λ
max (pH 7, NRC ): 262 nm
εmax
(pH 7, NRC ): 10.0 x 103
ε260
(pH 7, DBR ): 9.9 x 103
Storage: -20°CCAS #19817-92-6
23160 100mg 500mg 1gm
Biochemicals
Biochemicals
1�3w w w . u s b w e b . c o m
Uridylic AcidSee Uridine Monophosphate, page 306
Urocanic AcidC
6H
6N
2O
2
Formula Weight: 138.12PRODUCT SPECIFICATIONSForm: White powderAssay: 98.0 - 101.0%Chromatography (TLC): HomogeneousLoss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.2%Chloride (Cl): ≤ 0.10%Arsenic (As
2O
3): ≤ 2 ppm
Heavy Metals (Pb): ≤ 10 ppmCAS #104-98-3
23170 1gm 5gm
Urokinase(E.C.3.4.99.26)Source: Human UrineStorage: -20°CCAS #9039-53-6
23715 10,000units
UTPSee Uridine-5'-Triphosphate, page 306
DL-Valine(CH
3)2CHCH(NH
2)COOH
Formula Weight: 117.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%Chromatography (TLC): Homogeneous[α]20
D : 0° ± 2° (c=8, 6N HCl)
Identity: By IRLoss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Ammonium (NH
4): ≤ 0.02%
Chloride (Cl): ≤ 0.02%Sulfate (SO
4): ≤ 0.05%
Iron (Fe): ≤ 10 ppmHeavy Metals (Pb): ≤ 10 ppmCAS #516-06-3
23195 100gm 1kg 5kg
L-ValineUSBioAnalyzed(CH
3)2CHCH(NH
2)COOH
Formula Weight: 117.15PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 98.5 - 101.5% (dry basis)Identity: By IRpH (5%, H
2O): 5.5 - 7.0
[α]25D : +26.6° to +28.8° (c=8 in 6N HCl)
Loss on Drying: ≤ 0.3%Residue on Ignition: ≤ 0.1%Chloride (Cl): ≤ 0.05%Iron (Fe): ≤ 0.003%Sulfate (SO
4): ≤ 0.03%
Heavy Metals (Pb): ≤ 0.0015%Chromatographic Purity: Passes testResidual Solvents: Meets requirementsCAS #72-18-4
23205 10gm 25gm 100gm 500gm 1kg
ValinomycinC
54H
90O
18N
6
Formula Weight: 1111.32PRODUCT SPECIFICATIONSForm: White to off-white powderAssay (HPLC): ≥ 93.0% (as is)Chromatography (TLC): HomogeneousIdentity: By IR[α]20
D : +31° (c=1.6 in benzene)
Note: This is a highly toxic material; use extreme care when handling.
Storage: 4°CCAS #2001-95-8
23219 5mg 10mg 25mg
Vancomycin, HydrochlorideUSBioAnalyzedC
66H
75Cl
2N
9O
24•HCl
Formula Weight: 1485.71PRODUCT SPECIFICATIONSForm: Tan to brown powderActivity: ≥ 900 µg of vancomycin/mg (dry basis) ≥ 855 µg of vancomycin/mg (as is)Identity: By IRChromatographic Purity: Vancomycin B: ≥ 80.0% Other Peaks: ≤ 9%pH (50 mg/ml, H
2O): 2.5 - 4.5
Moisture (KF): ≤ 5.0%Storage: 4°C, in tight containers.CAS #1404-93-9
23261 100mg 250mg
VitafreeTM CaseinSee Casein Vitafree, page 203
Vitamin A AcetateC
22H
32O
2
Formula Weight: 328.5PRODUCT SPECIFICATIONSForm: Light yellow, fine granular powder.
Individual particles contain all-trans-Vitamin A Acetate finely dispersed in a starch coated matrix of gelatin, sucrose and corn starch. BHT is added as an antioxidant.
Assay: ≥ 500,000 IU/gmLoss on Drying: ≤ 8.0%Storage: 4°CCAS #127-47-9
23310 25gm 100gm
Vitamin B1
See Thiamine HCl, page 296
Vitamin B2
See Riboflavin, page 283
Bioc
hem
ical
sBiochemicals
1�� P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Vitamin B5
See D, DL Calcium Pantothenate, page 202
Vitamin B6
See Pyridoxine, HCl, page 280
Vitamin B7
See DL-Carnitine HCl, page 203
Vitamin B12
0.1% trituration in gelatinPRODUCT SPECIFICATIONSForm: Tan/slight pink powderComposition: Vitamin B
12: 0.1%
Gelatin: 99.9%
23365 10gm 25gm 100gm
Vitamin B12
(Cyanocobalamin)
USBioAnalyzedC
63H
88CoN
14O
14P
Formula Weight: 1355.37PRODUCT SPECIFICATIONSForm: Dark red crystalline powderAssay: 96.0 - 100.5% C
63H
88CoN
14O
14P
(dry basis)Identity (A, B, C): Passes testsLoss on Drying: ≤ 12.0%Psuedocyanocobalamin: Passes testStorage: In tight, light-resistant containersCAS #68-19-9
23360 250mg 500mg 1gm
Vitamin B12
0.1% trituration in mannitolPRODUCT SPECIFICATIONSForm: Tan/slight pink powderComposition: Vitamin B
12: 0.1%
Mannitol: 99.9%
23370 10gm 25gm 100gm
Vitamin CSee L-Ascorbic Acid, page 193
Vitamin D3
See Cholecalciferol, page 206
Vitamin Free CaseinSee Casein-Vitafree, page 203
Vitamin GSee Riboflavin, page 283
Vitamin HSee D-Biotin, page 197
Vitamin K1
(Phytonadione; 2-methyl-3-Phytyl-3-1; 4-Napthoquinone)
USBioAnalyzedC
31H
46O
2
Formula Weight: 450.70PRODUCT SPECIFICATIONSForm: Clear yellow to amber viscous liquidAssay: 97.0 - 103.0%Identity (A, B): Passes testsRefractive Index (25°C): 1.523 to 1.526Reaction: Neutral to litmusLimit of Menadione: Passes testZ-isomer content: ≤ 21.0%CAS #84-80-0
23445 250mg 1gm 5gm 10gm
Vitamin K3
See Menadione, page 254
Vitamin K5
(2-Methyl-4-Amino-1-Napthol HCl; 2-Methyl-3-Phytyl-3-1, 4-Napthoquinone)
C11
H11
NO•HClFormula Weight: 209.67Storage: -20°CCAS #83-70-5
23446 1gm 5gm
Water, Nuclease-Free
ULTRAPUREMB GradeForm: High purity dH
2O tested for contaminating
nuclease activity. Product is 0.2 µm filtered and dispensed into vials and/or durable, square Nalgene bottles. The 5 L size is supplied in an upright carton with a convenient dispensing spout.
Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes test Protease: Passes test Enzyme Inhibition: Passes test PCR Test: Passes test
71786 10x1ml 100ml 500ml 1L 5L
Water, Nuclease-Free, Endotoxin-Tested
ULTRAPUREMB GradeForm: High purity dH
2O tested for contaminating
nuclease and endotoxin activity. Product is 0.2 µm filtered and dispensed into durable, square Nalgene bottles.
Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes test Endotoxin: ≤ 0.25 EU/ml
71788 100ml 1L
Biochemicals
Biochemicals
1�5w w w . u s b w e b . c o m
Water, RNase-Free, DEPC Treated
ULTRAPUREMB GradeForm: High purity dH
2O treated overnight with
0.1% Diethylpyrocarbonate. Product is 0.2 µm filtered and dispensed into vials and/or durable, square Nalgene bottles, then autoclaved.
Contaminant Testing: RNase: Passes test DNase (endo): Passes test DNase (exo): Passes test Protease: Passes test Enzyme Inhibition: Passes test PCR Test: Passes test
70783 25ml 10x1ml 100ml 500ml 1L
WGASee Triticum Vulgaris Agglutinin, page 303
Wheat GermPRODUCT SPECIFICATIONSForm: Tan granular powderProtein: 25 - 30%Fat: ≥ 7%Free Fatty Acids: ≤ 14%Moisture: ≤ 13.5%Fines: ≤ 12% (% through 80 mesh)Storage: Ambient, in tight containers
23451 5lb 25lb 100lb
Wheat Germ AgglutininSee Triticarium Vulgaris Agglutinin, page 303
Wright’s StainPrepared for use in blood stain work.CAS #68988-92-1
23457 5gm 10gm 25gm 100gm
XanthineC
5H
4N
4O
2
Formula Weight: 152.11PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (Total UV): ≥ 98% (dry basis)Chromatography (TLC): HomogeneousIdentity: By IRλ
max: 284 nm ± 1 nm (1N NaOH)
εmax
:[9.4 ± 0.2] x 103 (1N NaOH)Spectral Ratios (1N NaOH): A
250/A
260: 1.11 ± 0.04
A280
/A260
: 2.39 ± 0.04Loss on Drying: ≤ 1.0%Heavy Metals: ≤ 10 ppmReference Constants:λ
max (pH 10, NRC ): 277 nm
εmax
(pH 10, NRC ): 9.3 x 103
ε260
(pH 10, DBR ): 5.2 x 103
CAS #69-89-6
23460 5gm 10gm 25gm 100gm 1kg
Xanthine Monosodium Salt, Monohydrate
C5H
3N
4O
2Na•H
2O
Formula Weight: 192.11PRODUCT SPECIFICATIONSForm: White to off-white crystalline powderAssay (Total UV): ≥ 96.0% (dry basis)Identity: By IRMoisture (KF): ≤ 12.0%Spectral Ratios (pH 10.0): A
250/A
260: 1.29 ± 0.06
A280
/A260
: 1.71 ± 0.05Reference Constants:λ
max (pH 10, NRC ): 277 nm
εmax
(pH 10, NRC ): 9.3 x 103
ε260
(pH 10, DBR ): 5.2 x 103
CAS #1196-43-6
23465 1gm 5gm 10gm 25gm 100gm
Xanthine Oxidase(E.C.1.2.3.2)Source: ButtermilkForm: Ammonium sulfate suspensionStorage: 4°C, do not freeze.
23470 40units 200units
Xanthosine, DihydrateC
10H
12N
4O
6•2H
2O
Formula Weight: 320.26Reference Constants:λ
max (pH 8, NRC ): 278 nm
εmax
(pH 8, NRC ): 8.9 x 103
ε260
(pH 8, DBR ): 7.7 x 103
CAS #5968-90-1
23485 2gm 5gm 10gm
X-α-GALSee 5-Bromo-4-Chloro-3-Indolyl-α-D-
Galactopyranoside, page 199
X-GalSee 5-Bromo-4-Chloro-3-Indolyl-β-D-
Galactopyranoside, page 199
X-GlucSee 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronic
Acid, page 200
Bioc
hem
ical
sBiochemicals
1�6 P h o n e : 1 - 8 0 0 - 3 � 1 - 9 3 � � | � 1 6 - 7 6 5 - 5 0 0 0
Xylene Cyanol FF
ULTRAPURE(C.I.43535)C
25H
27N
2NaO
7S
2
Formula Weight: 554.61PRODUCT SPECIFICATIONSForm: Dark burgundy metallic powderIdentity: By IRε
max (615 nm, H
2O, 1 cm): ≥ 60 x 103
Loss on Drying: ≤ 10.0%λ
max (H
2O solution): 615 nm ± 2 nm
Application:•Useful as a tracking dye during the separation of
nucleic acids (in particular, in DNA sequencing electrophoresis)
Sequencing Stop Solution: 95% Formamide 20mM EDTA 0.05% Bromophenol Blue 0.05% Xylene Cyanol FF Store solution at -20°C.10X Agarose Gel Loading Solution: 50% Glycerol 60mM EDTA 0.05% Bromophenol Blue 0.05% Xylene Cyanol 1.0% SDSStorage: Store solution at room temperature.CAS #4463-44-9
23513 25gm
Xylitol(Xylite)
C5H
12O
5
Formula Weight: 152.15PRODUCT SPECIFICATIONSForm: White powderAssay: 98.5 - 101.0% (dry basis)Identity: By IRMoisture (KF): ≤ 0.5%Residue on Ignition: ≤ 0.5%Arsenic (As): ≤ 3 ppmHeavy Metals (Pb): ≤ 10 ppmReducing Sugars: ≤ 0.2%, as dextroseOther Polyols: ≤ 2.0%CAS #87-99-0
23515 100gm 1kg
D-(+)-XyloseC
5H
10O
5
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 99.0%[α]20
D : +18.9° to +19.7° (c=4 in H
2O)
Loss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.05%Heavy Metals (Pb): ≤ 5 ppmChloride (Cl): ≤ 50 ppmSulfate (SO
4): ≤ 50 pm
CAS #58-86-6
23520 100gm 500gm 1kg
D-(+)-XyloseC
5H
10O
5
Formula Weight: 150.13PRODUCT SPECIFICATIONSForm: Off white to slightly yellow crystalline
powder[α]20
D : +18.0° ± 2.0° (c=4 in H
2O)
Loss on Drying: ≤ 1.0%Residue on Ignition: ≤ 0.05%CAS #58-86-6
23522 500gm 1kg
L-XyloseC
5H
10O
5
Formula Weight: 150.13CAS #609-06-3
23530 5gm 10gm
Yeast, BakersSource: Baker’s YeastStorage: 4°C
23540 1lb 5lb 25lb
Yeast, BrewersCAS #68876-77-7
23546 1lb 5lb 25lb 100lb
Yeast Extract IISource: Baker’s YeastPRODUCT SPECIFICATIONSForm: Off-white to tan powderAmino Nitrogen: ≥ 4.5%Total Nitrogen: ≥ 10%Loss on Drying: ≤ 5% (at time of packaging)Ash: ≤ 15%Standard Plate Count: ≤5,000/gmSalmonella: NegativeThis product is hygroscopic.
23542
Yeast Extract Powder(Peptones)
ULTRAPURESource: Baker’s YeastPRODUCT SPECIFICATIONSForm: Off-white powderProtein (TN x 6.25): 57-72%Moisture: ≤ 6% at time of packagingSalmonella: NegativeStandard Plate Count: ≤ 15,000/gmThis product is hygroscopic.Storage: Ambient, in tightly closed containers.CAS #8013-01-2
23547 500gm 1kg
Yeast Extract Powder, Low Dust (Peptones)
Source: Baker’s YeastPRODUCT SPECIFICATIONSForm: Off-white powder, low dustProtein (TN x 6.25): 57 - 72%Moisture: ≤ 6% at time of packagingSalmonella: NegativeStandard Plate Count: ≤ 15,000/gmThis product is hygroscopic.Storage: Ambient, in tightly closed containerCAS #8013-01-2
23545 1kg 5kg
Yeast Extract Ultra Filtered
23549 250gm 1kg 5kg
Biochemicals
Biochemicals
1�7w w w . u s b w e b . c o m
Yeast Hydrolysate, Enzymatic(Peptones)
ULTRAPUREPRODUCT SPECIFICATIONSForm: Light tan powderIdentity: By IRSolubility (5%, H
2O): Passes test
pH (5% H2O): 5.4 - 6.2
CAS #100684-36-4
23550 1lb 5lb 25lb 50lb 100lb
Yeast Hydrolysate, Enzymatic, Low Dust(Peptones)
PRODUCT SPECIFICATIONSForm: Light tan powder, low dustIdentity: By IRSolubility (5%, H
2O): Passes test
pH (5% H2O): 5.4 - 6.2
CAS #100684-36-4
23558 1kg 5kg
Yeast Thioredoxin Reductase (NAPH), Recombinant(r-yNTR)
Source: Yeast recombinant in E. coliPRODUCT SPECIFICATIONSForm: Lyophilized powder from phosphate buffer
(20mM NaH2PO
4 pH 7.4 - 0.15M NaCl)
Activity: ≥ 5 units/mg proteinPurity: ≥ 98% as determined by SDS-PAGEFormula Weight: 36,000Unit Definition: One unit equals the change in
absorbance at 412 nm per minute at 25°C using 0.2mM NADPH containing 5mM DTNB (pH 7.0)
Storage: -20°C, protect from moisture
26116 100µg
Yeast-Torula
23551 5lb 25lb 100lb
YPD Agar, Ready-Made PowderForm: Light beige, free flowing powderpH (6.5%, H
2O): 6.5 ± 0.2
Contents: Casein Peptone: 20 gm/L Yeast Extract: 10 gm/L D-Glucose: 20 gm/L Agar: 15 gm/LTo use: Dissolve 65 gm powder per liter of
purified water. Autoclave, cool and make plates.
Application: •For the propagation of yeast
75857 250gm 1kg
YPD Broth, Ready-Made PowderForm: Off-white free flowing powderpH (5.0%, H
2O): 6.5 ± 0.2
Contents: Casein Peptone: 20 gm/L Yeast Extract: 10 gm/L D-Glucose: 20 gm/LTo use: Dissolve 50 gm powder per liter of
purified water. Autoclave.Application: •For the propagation of yeast
75858 250gm 1kg
2X YT AgarForm: Off-white, free-flowing powderpH (4.6%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 16 gm/L Yeast Extract: 10 gm/L Sodium Chloride: 5 gm/L Agar: 15 gm/LTo Use: Dissolve 46 gm powder per liter of
purified water.
75865 1kg
2X YT BrothForm: Off-white, free-flowing powderpH (3.1%, H
2O): 7.0 ± 0.2
Contents: Casein Peptone: 16 gm/L Yeast Extract: 10 gm/L Sodium Chloride: 5 gm/LTo Use: Dissolve 31 gm powder per liter of
purified water.
75864 1kg
ZeinCAS #9010-66-6
23560 1lb 5lb 25lb
Zinc ChlorideACS Reagent GradeZnCl
2
Formula Weight: 136.32PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: ≥ 97.0%Oxychloride: Passes testInsoluble Matter: ≤ 0.005%Nitrate (NO
3): ≤ 0.003%
Sulfate (SO4): ≤ 0.01%
Ammonium (NH4): ≤ 0.005%
Calcium (Ca): ≤ 0.06%Iron (Fe): ≤ 0.001%Lead (Pb): ≤ 0.005%Magnesium (Mg): ≤ 0.01%Potassium (K): ≤ 0.02%Sodium (Na): ≤ 0.05%
23562 500gm 2.5kg
Zinc Sulfate, HeptahydrateACS Reagent GradeZnSO
4•7H
2O
Formula Weight: 287.58PRODUCT SPECIFICATIONSForm: White crystalline powderAssay: 99.0 - 103.0%pH (5%, H
2O, 25°C): 4.4 - 6.0
Insoluble Matter: ≤ 0.01%Chloride (Cl): ≤ 5 ppmNitrate (NO
3): ≤ 0.002%
Ammonium (NH4): ≤ 0.001%
Calcium (Ca): ≤ 0.005%Iron (Fe): ≤ 0.001%Lead (Pb): ≤ 0.003%Magnesium (Mg): ≤ 0.005%Manganese (Mn): ≤ 3 ppmPotassium (K): ≤ 0.01%Sodium (Na): ≤ 0.05%Storage: Ambient, in tight containers.
25024 500gm 2.5kg
Zwittergent® DetergentsSee Sulfobetaine 8 (SB3-8), page 292See Sulfobetaine 10 (SB3-10), page 292See Sulfobetaine 12 (SB3-12), page 293See Sulfobetaine 14 (SB3-14), page 293See Sulfobetaine 16 (SB3-16), page 293See Sulfobetaine 18 (SB3-18), page 293
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