Bio-imprinting Bio-imprinting The advance of recent work Mu yangyang The ENZYME ENGINEERING.
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Bio-imprinting The advance of recent work Mu yangyang The ENZYME ENGINEERING
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Transcript of Bio-imprinting Bio-imprinting The advance of recent work Mu yangyang The ENZYME ENGINEERING.
Bio-imprinting
The advance of recent work
Mu yangyang
The ENZYME ENGINEERING
The ENZYME ENGINEERING
Contents
Recent developments1
Further plan2
Paper sharing3
Abstract
• Based on Vinyl Acetate
• Based on (+)-Ethyl Mandelate
Template : vinyl acetate + R-(-)-mandelic acid
a. Template : (+)-Ethyl Mandelate b. Template : (+)-Ethyl Mandelate + 1-phenylethanol
The ENZYME ENGINEERING
Project
Template : Vinyl Acetate + R-(-)-Mandelic Acid
Conditions: Detecting per 2h
Results: No products were detected after 12h.
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Abs
orpt
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Time (min)
0 h 12 h
a. Template : (+)-Ethyl Mandelate
Conditions: Detecting per 2h
Results: The promising results were abserved.
The ENZYME ENGINEERING
Project
a. Template : (+)-Ethyl Mandelate
Conditions: Detecting per 2h
Results: The promising results were abserved.
The ENZYME ENGINEERING
Project
Hot Tip
The ENZYME ENGINEERING
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Abso
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Time (min)
0 h 12 h
Results: R-(+)-1-phenylethanol reduced obviously, while the S-(-)-1-phenylethanol barely reacted.
The conversion of the substrate was 17.2% and the eep>99%.
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Project
b. Template : (+)-Ethyl Mandelate + (R,S)-1-phenylethanol
Results: The results were also inspiring. The conversion of the substrate was 22.7% and the eep>99%.
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rptio
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12 h 0 h
Comparison
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Template substrate
eep (%) Conversion (%)
EP
None 85 2.3 13
(+)-ethyl mandelate
>99 17.2 1372
Imprinting with (+)-ethyl mandelate and 1-
Phenylethanol>99 22.7 5049
The ENZYME ENGINEERING
Contents
Recent developments1
Further plan2
Paper sharing3
The ENZYME ENGINEERING
Further work
To verify the hypothesis of bio-imprinting process in accordance with compulsory-order mechanism, the following work should be done.
Imprinted with R-(+)-1-phenylethanol
Imprinted with S-(-)-1-phenylethanol
Imprinted with Vinyl Acetate and R-(+)-1-phenylethanol / S-(-)-1-phenylethanol
The ENZYME ENGINEERING
Contents
Recent developments1
Further plan2
Paper sharing3
The ENZYME ENGINEERING
Paper
The STYLE of your expression in the paper was quite essential.
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Highlight
The E values didn’t depend on the catalytic constants involved in the acylation step.
The sensitivity of the enantioselectivity was related to the enantiomeric form of the leaving alcohol, indicate that the imprinting effect involves modifications near the active site.
The ENZYME ENGINEERING
Paper
The ENZYME ENGINEERING
Asp and Glu with acidic residues were modified with EDC.
Chemical modification did not have a direct effect on chemical groups related to the enzyme activity, but may be altering the percentage or the shape of the open form of the lipase.
Combining the immobilization and amination seems to be a very useful tool in the combinatorial biocatalysis, which can change the characteristics of the enzyme like specific activity and enantioselectivity.
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