Bio f4 Chap 3 Movement of Substances Across the Plasma Membrane
Transcript of Bio f4 Chap 3 Movement of Substances Across the Plasma Membrane
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MOVEMENT OF SUBSTANCES ACROSS
THE PLASMA MEMBRANE
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The necessity for movement of
substances across the plasma
membraneAll movement of substances go through the cellmembrane , which is also known as the plasmamembrane .The necessity for the movement of substancesacross a plasma membrane are:
a) Cells need nutrients and oxygen.b) Cells produce waste product which exit through the
plasma membrane.c) The plasma membrane control the types and the
amounts of substances needed by the cell at anyone time.
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Cytoplasm
Nucleus
Plasmamembrane
Ext ernal environmen t
C ell
Movement of substancesout of the cell
Movement of substancesinto the cell
Figure 3.1: Movement
of substances in and ou
tof
the cell
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The structure of theplasma membrane
Singer and Nicholson proposed the fluid mosaic model in1972 to explain the structure of the plasma membraneThe fluid mosaic model is the currently accepted model of thecell membrane.The basic unit of the plasma membrane is the phospholipidmolecule.The phospholipid molecule consists of:
a) A polar molecule carries an unequal distribution of electriccharge. This unequal distribution of electric charge produce apolar molecule which can attract other polar molecule such as
water molecules.b) Hydrop h ilic means water-loving or attracted to water molecule
c) Hydrop h obic water-hating , or repelling water molecules.
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Phospholipid units (polar head) attract each other.One layer of phospholipid form over another to produce aphospholipid bilayer
In this phospholipid bilayer the:a) Hydrophilic heads points outwards facing water molecule onboth sides.
b) Hydrophobic tails points inwards , away from water molecules.
Other molecule present in the plasma membrane are:a) Ch oles t erol molecules which fit in between the phospholipid
molecules , making the plasma membrane more rigid ands t able .
b) C arrier pro t ein and c h annel pro t ein which assist and control
the movement of water-soluble ions and certain moleculesacross the membrane.c) Glycolipids which are combination of lipids and
polysacc h arides , help cells to recognise each other.d) Glycopro t ein which are combination of pro t ein and
polysacch
arides , also help cells to recognise each other.
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Permeability of the plasma membranePermeability of the phospholipid bilayer.
1. Permeable means allowing something to pass through.2. The plasma membrane is selective permeable or semi-permeable as
it allows only certain substances to pass through it but not others.3. The phospholipid bilayer is permeable to:a) Small non-polar (hydrophobic) molecules that are lipid-soluble, such
as fatty acids, glycerol, steroid, vitamin A, D, E and K.b) Small unchanged molecules, such as water, oxygen and carbon
dioxide. These molecules are small enough to squeeze throughbetween the phospholipid gaps by simple diffusion or osmosisdown their respective concentration gradients.
4. The phospholipid bilayer is not permeable to:a) Large polar molecule, that are not soluble in lipid, such as glucose,
amino acids, nucleic acids and polysaccharides.b) Ions (charged), regardless of size, such as: H +, Na +, HCO 3-, K +, Ca +,
and Mg +
3
3
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Types of transport across the plasma
membrane.1. Solute move across the plasma membrane by two mainprocess:
a) Passive transport- which does not require a cell to useenergy, and
b) Active transport- which requires a cell to use energyto move molecules through its cell membrane.
2. Passive transport consists of:a) Simple diffusion and
b) Facilitated diffusion (aided by carrier protein orchannel protein of the plasma protein).3. Osmosis is the special name given to simple diffusion
of water molecules through a semi-permeablemembrane.
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SIMPLE DIFFUSION
1. Simple diffusion is the random movement of ions or moleculesfrom a region of their high concentration to a region of theirlow concentration down a concentration gradient until anequilibrium is achieved.
2. Molecules have kinetic energy, move randomly, and collidewith each other.3. There are more collision in a region of high concentration than
in a region of low concentration.4. Random collision of molecules spread the molecules out, down
the concentration gradient.
Concentration gradient = difference inconcentration of a particular substance inone region compared to another region
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F actors affecting the rate of
diffusion
Factor Effect on the rate of simplediffusion
Diffusion gradient The steeper, the higher the rate
Size of moleculesor ions
The smaller the size, the higher the rate
Temperature The higher the temperature, the
higher the rateDiffusion medium Rate in gas > rate in liquid > rate
in solid
Surface area The larger the surface area, thehigher the rate
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D istilled
water Potassiummanganate(vii) crystals
a) T h e crys t als s t ar t s t odissolve, forming a region of h ig h concen t ra t ion of solu t emolecules.
b) T h e moleculesdiffuse ou t alongth e concen t ra t iongradien t in alldirec t ion.
c) E ven t ually th emolecules spreadth roug h ou t th e wa t er uniformly.
Purplesolution
A sample e x perimen t t o illus t ra t e th e p h ysical process of diffusion
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F acilitated diffusion
Facilitated diffusion is the movement of specific molecules (or ions) across theplasma membrane.Facilitated diffusion is assisted either by pore proteins or by carrier protein, and
the direction of movement is down the
concentration gradient of the moleculesconcerned. No energy required.
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The function of pore protein and carrier protein:
a) Pore protein (channel protein)i. Charge ions (such as Na +, K +, Ca +, and Mg +) cannot
diffuse across the non-polar center of thephospholipids bilayer.
ii. Pore proteins open up pores or channel across themembrane to allow entry or exit.
iii. Each pore or channel is specific and will only allow oneparticular type of ion through
b) Carrier proteinsi. They allow larger polar molecules (such as sugar and
amino acids) to pass through.ii. A particular protein attaches itself to the binding site of
a carrier protein.iii. Then the carrier protein changes shape and delivers the
molecule across the plasma membrane.
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OSMOSISO smosis is the movement of water
molecules from region of high water
concentration to low water
concentration through a semi-
permeable membrane.
O smosis is the diffusion of water only
and not of the substances that dissolved in water.
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ACTIVE TRANSPORTActive transport is the movement of particles across
the plasma membrane against the concentrationgradient, that is from a region of low concentration toa region of high concentration.Energy is provided by adenosine triphosphate (ATP)
molecules.Active transport also required a specific carrier
protein to carries molecules in or out of the cell.Active transport only take place in living organisms.Examples of active transport in biology:
a) Absorption of dissolved mineral salt by root hairs.b) Absorption of glucose and amino acids by cell in the
small intestine.
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HYPOTONIC, HYPERTONIC ANDISOTONIC SOLUTION
1 . ISO means the same as and tonicity refers to thestrength (concentration of solute) of the solution.Two solution are isotonic if they have the same
solute concentrations.2. HYPER means more than. Solution A is hypertonic
to solution B if solution A has a higher soluteconcentration than solution B.
3. HYPO means less than. Solution A is hypotonic tosolution B if solution A has a lo wer soluteconcentration than solution B
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Hypertonic solution Isotonic solution Hypotonic solution Remains of cellsurface membrane
Crenation : Redblood cell shrinksand turns spiky
Red blood cell Swells up Haemolysis : Redblood cell finally burst
CRENATION AND HAEMOLYSIS OF RED BLOOD CELL
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G ASEOUS EXCHAN G E IN THE ALVEOLI ANDBLOOD CAPILLARIES BY SIMPLE DIFFUSION
1. In the lungs , capillary blood takes in oxygen from thealveolar air space.
2. Alveolar oxygen diffused across the alveolar wallsand the capillary walls into the capillary blood , downthe oxygen concentration gradient.
3. The blood circulation system takes the oxygen richblood away and replaces it with blood low in oxygen ,
but high in carbon dioxide.4. Carbon dioxide from the capillary blood diffusedacross the capillary and the alveolar walls into thealveolar air spaces , down the carbon dioxide
concentration gradient.
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Inhaled and exhaled air
O2 CO 2
Oxygenated bloodDeoxygenatedblood
Red blood cell
Capillary wall
Alveolus wall
Air sac
GASEOUS EXHANGE BY SIMPLE DIFFUSION IN THE ALVEOLUS
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THE EFFECT OF
HYPOTONIC,HYPERTONIC ANDISOTONIC SOLUTION ON
PLANT CELL ANDANIMAL CELL
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PRESERVA TI ON OF FOOD U S I NG SAL T O R S U G AR
1 . Food goes bad due to bacterial and fungal activities .
2. To make food last for a long time, we preserve them.3. Here, we shall look at how osmosis help us preserve our food
4. A concentrate salt solution has a high concentration of salute (Na+ and Cl - ions) and very low concentration of
water molecules.5. When we leave food in a concentrated salt solution or thewater molecules within the food cells a drawn out by osmosis, making the food really dry .
6. Without water bacteria and fungus cannot survive7. The same reason goes for why we use sugar to preserve
our food, too.8. The only different is in our concentrate sugar solution,
sugar molecules from the high concentration of solute, witha very low concentration of water molecules.